cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_4ZB-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1827.678 CPU sec, 63.600 real sec [M::process] read 706966 sequences (100000053 bp) in 1.602 CPU sec, 1.610 real sec... [M::process] read 706780 sequences (100000242 bp) in 1.850 CPU sec, 1.843 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 289476, 15, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 238, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (280.54, 137.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 217, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (240.95, 118.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 402, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (477.80, 300.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2032) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 278, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2266) [M::mem_pestat] mean and std.dev: (352.11, 310.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706236 sequences (100000263 bp) in 1.420 CPU sec, 1.429 real sec... [M::process] read 705422 sequences (100000023 bp) in 1.888 CPU sec, 1.906 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 289297, 30, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 236, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (258.02, 129.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (241.49, 118.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 475, 2464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7326) [M::mem_pestat] mean and std.dev: (1103.72, 1299.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9757) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 224, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1926) [M::mem_pestat] mean and std.dev: (403.52, 369.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706966 reads in 209.508 CPU sec, 6.654 real sec [M::mem_process_seqs] Processed 706780 reads in 138.870 CPU sec, 4.480 real sec sorting block 0, #reads = 1088309 mem = 536877665 peak = 598462464 [M::process] read 704416 sequences (100000286 bp) in 2.493 CPU sec, 2.563 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 288641, 26, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 308, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (293.81, 158.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 219, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (242.67, 119.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 977, 1607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4013) [M::mem_pestat] mean and std.dev: (1054.92, 782.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 366, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (501.06, 438.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703904 sequences (100000117 bp) in 1.910 CPU sec, 1.916 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288511, 38, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 397, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215) [M::mem_pestat] mean and std.dev: (384.98, 234.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 221, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (244.98, 121.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (203, 816, 1680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4634) [M::mem_pestat] mean and std.dev: (1032.43, 975.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 318, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (309.90, 177.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 288559, 19, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 268, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (258.26, 135.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (247.40, 122.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (492, 1380, 2796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7404) [M::mem_pestat] mean and std.dev: (1537.21, 1255.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 281, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (293.10, 145.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706236 reads in 288.113 CPU sec, 9.346 real sec [M::mem_process_seqs] Processed 705422 reads in 226.708 CPU sec, 7.433 real sec [M::mem_process_seqs] Processed 704416 reads in 253.108 CPU sec, 8.317 real sec sorting block 1, #reads = 1086081 mem = 536881318 peak = 725864448 [M::process] read 703854 sequences (100000082 bp) in 2.694 CPU sec, 3.034 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 287934, 20, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 237, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (273.68, 131.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (248.95, 123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (563, 1232, 1864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4466) [M::mem_pestat] mean and std.dev: (1233.68, 856.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 265, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (265.62, 122.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 2, #reads = 1085153 mem = 536878505 peak = 923742208 [M::process] read 703762 sequences (100000060 bp) in 2.436 CPU sec, 2.546 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288191, 22, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 331, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (359.96, 219.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (248.72, 123.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (362, 621, 2067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5477) [M::mem_pestat] mean and std.dev: (1262.77, 1430.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7182) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 238, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (261.09, 108.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703904 reads in 207.137 CPU sec, 6.657 real sec [M::process] read 703486 sequences (100000044 bp) in 2.212 CPU sec, 2.276 real sec... [M::mem_process_seqs] Processed 703854 reads in 149.254 CPU sec, 4.741 real sec sorting block 3, #reads = 1085387 mem = 536882633 peak = 923742208 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 287876, 36, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 211, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (283.93, 207.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (249.32, 124.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 620, 2279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6247) [M::mem_pestat] mean and std.dev: (973.34, 1236.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8231) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 309, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (484.21, 487.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703362 sequences (100000058 bp) in 2.622 CPU sec, 2.785 real sec... [M::process] read 703410 sequences (100000095 bp) in 1.926 CPU sec, 1.989 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 287750, 29, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 258, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (295.17, 181.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (248.67, 122.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 739, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (260, 1210) [M::mem_pestat] mean and std.dev: (738.74, 89.13) [M::mem_pestat] low and high boundaries for proper pairs: (70, 1400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 260, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254) [M::mem_pestat] mean and std.dev: (299.72, 164.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703762 reads in 205.089 CPU sec, 6.585 real sec [M::mem_process_seqs] Processed 703486 reads in 142.594 CPU sec, 4.574 real sec sorting block 4, #reads = 1084249 mem = 536879820 peak = 923742208 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 287653, 39, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 236, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (266.61, 126.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (247.33, 121.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 417, 967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2381) [M::mem_pestat] mean and std.dev: (567.14, 568.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 274, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (306.98, 176.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703592 sequences (100000221 bp) in 2.458 CPU sec, 2.714 real sec... [M::process] read 703178 sequences (100000293 bp) in 2.094 CPU sec, 2.101 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 287490, 28, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 228, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1664) [M::mem_pestat] mean and std.dev: (385.38, 319.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 223, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (247.10, 121.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (843, 990, 2239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5031) [M::mem_pestat] mean and std.dev: (1069.88, 834.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6427) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (256.45, 101.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703362 reads in 210.577 CPU sec, 7.027 real sec sorting block 5, #reads = 1084474 mem = 536880416 peak = 937795584 [M::mem_process_seqs] Processed 703410 reads in 142.388 CPU sec, 4.669 real sec sorting block 6, #reads = 1083930 mem = 536877360 peak = 937795584 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 287838, 30, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 270, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742) [M::mem_pestat] mean and std.dev: (356.80, 291.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (247.61, 122.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (384, 1314, 1372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3348) [M::mem_pestat] mean and std.dev: (1010.29, 761.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 430, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2187) [M::mem_pestat] mean and std.dev: (514.12, 449.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702382 sequences (100000047 bp) in 2.760 CPU sec, 3.165 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 287965, 15, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 253, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (246.04, 144.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (249.28, 123.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (503, 568, 1020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2054) [M::mem_pestat] mean and std.dev: (531.58, 379.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 267, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1710) [M::mem_pestat] mean and std.dev: (450.14, 418.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701762 sequences (100000206 bp) in 2.229 CPU sec, 2.241 real sec... [M::mem_process_seqs] Processed 703592 reads in 210.742 CPU sec, 6.857 real sec [M::mem_process_seqs] Processed 703178 reads in 143.204 CPU sec, 4.666 real sec sorting block 7, #reads = 1083336 mem = 536878431 peak = 937795584 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 287371, 26, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 200, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (279.34, 237.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 226, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (249.46, 122.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 823, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3913) [M::mem_pestat] mean and std.dev: (594.10, 533.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5146) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 253, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416) [M::mem_pestat] mean and std.dev: (314.70, 275.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701588 sequences (100000076 bp) in 2.866 CPU sec, 3.227 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 287146, 31, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 280, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (350.22, 266.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 228, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (251.33, 123.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (131, 325, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2351) [M::mem_pestat] mean and std.dev: (658.23, 726.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 253, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (250.91, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701480 sequences (100000151 bp) in 2.128 CPU sec, 2.130 real sec... [M::mem_process_seqs] Processed 702382 reads in 211.335 CPU sec, 6.869 real sec [M::mem_process_seqs] Processed 701762 reads in 141.464 CPU sec, 4.637 real sec sorting block 8, #reads = 1083684 mem = 536880687 peak = 937795584 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 287424, 22, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 237, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1955) [M::mem_pestat] mean and std.dev: (392.29, 373.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (252.66, 123.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 1055, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4789) [M::mem_pestat] mean and std.dev: (878.37, 706.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 320, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1610) [M::mem_pestat] mean and std.dev: (392.76, 300.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701246 sequences (100000290 bp) in 2.934 CPU sec, 3.287 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 287084, 23, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 266, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (326.39, 194.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 230, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (254.21, 125.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (421, 566, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3190) [M::mem_pestat] mean and std.dev: (723.62, 551.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 330, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376) [M::mem_pestat] mean and std.dev: (356.08, 199.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702882 sequences (100000191 bp) in 2.293 CPU sec, 2.302 real sec... [M::mem_process_seqs] Processed 701588 reads in 208.277 CPU sec, 6.786 real sec sorting block 9, #reads = 1083099 mem = 536879699 peak = 937795584 [M::mem_process_seqs] Processed 701480 reads in 142.776 CPU sec, 4.568 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 287222, 23, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 211, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (247.85, 135.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (255.20, 126.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (366, 518, 1404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3480) [M::mem_pestat] mean and std.dev: (927.96, 810.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 397, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (380.32, 202.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 10, #reads = 1082545 mem = 536880914 peak = 937795584 [M::process] read 706958 sequences (100000059 bp) in 2.935 CPU sec, 3.460 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 287584, 24, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 262, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (242.67, 107.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 227, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (251.48, 124.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (187, 537, 2049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5773) [M::mem_pestat] mean and std.dev: (716.43, 732.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 254, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (239.26, 94.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706936 sequences (100000044 bp) in 2.390 CPU sec, 2.395 real sec... [M::mem_process_seqs] Processed 701246 reads in 209.035 CPU sec, 6.860 real sec [M::mem_process_seqs] Processed 702882 reads in 146.564 CPU sec, 4.740 real sec sorting block 11, #reads = 1083001 mem = 536880929 peak = 937795584 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 288719, 19, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 270, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (275.60, 151.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 217, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (241.76, 119.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (812, 1579, 4766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12674) [M::mem_pestat] mean and std.dev: (3477.47, 3282.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16628) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 217, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (231.09, 124.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706476 sequences (100000266 bp) in 2.912 CPU sec, 3.430 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 289022, 29, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 290, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3956) [M::mem_pestat] mean and std.dev: (585.38, 644.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 218, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (243.00, 121.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (370, 1052, 5338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15274) [M::mem_pestat] mean and std.dev: (2505.83, 2927.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 341, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1799) [M::mem_pestat] mean and std.dev: (419.24, 341.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706958 reads in 204.010 CPU sec, 6.645 real sec [M::process] read 705902 sequences (100000169 bp) in 2.534 CPU sec, 2.580 real sec... [M::mem_process_seqs] Processed 706936 reads in 142.929 CPU sec, 4.724 real sec sorting block 12, #reads = 1086560 mem = 536879130 peak = 937795584 [M::process] read 705000 sequences (100000066 bp) in 2.648 CPU sec, 3.109 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 288972, 14, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 339, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (299.61, 165.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 221, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (245.53, 122.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 606, 1527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4523) [M::mem_pestat] mean and std.dev: (720.92, 840.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6021) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 299, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2001) [M::mem_pestat] mean and std.dev: (430.51, 365.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704576 sequences (100000039 bp) in 2.321 CPU sec, 2.340 real sec... [M::mem_process_seqs] Processed 705902 reads in 121.128 CPU sec, 3.873 real sec sorting block 13, #reads = 1087040 mem = 536884987 peak = 937795584 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 289524, 16, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 278, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528) [M::mem_pestat] mean and std.dev: (334.72, 243.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 219, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (244.64, 122.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 450, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1493) [M::mem_pestat] mean and std.dev: (491.62, 274.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 468, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (391.12, 210.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288854, 21, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 218, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1534) [M::mem_pestat] mean and std.dev: (323.84, 303.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (247.36, 123.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (428, 547, 1783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4493) [M::mem_pestat] mean and std.dev: (794.78, 695.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 463, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907) [M::mem_pestat] mean and std.dev: (453.16, 302.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706476 reads in 318.217 CPU sec, 10.443 real sec [M::process] read 704776 sequences (100000060 bp) in 2.385 CPU sec, 2.446 real sec... [M::mem_process_seqs] Processed 705000 reads in 168.981 CPU sec, 5.621 real sec sorting block 14, #reads = 1086627 mem = 536876951 peak = 937795584 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 288361, 23, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 248, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (292.51, 189.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (249.99, 125.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 680, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2867) [M::mem_pestat] mean and std.dev: (610.81, 447.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3739) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 315, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027) [M::mem_pestat] mean and std.dev: (486.93, 420.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704324 sequences (100000297 bp) in 3.080 CPU sec, 3.424 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 288417, 22, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 276, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (300.82, 192.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (249.57, 125.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 871, 1496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4172) [M::mem_pestat] mean and std.dev: (747.90, 659.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 310, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (323.04, 158.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704576 reads in 205.640 CPU sec, 6.768 real sec [M::process] read 703906 sequences (100000072 bp) in 2.372 CPU sec, 2.443 real sec... sorting block 15, #reads = 1086872 mem = 536877226 peak = 937795584 [M::mem_process_seqs] Processed 704776 reads in 143.080 CPU sec, 4.751 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 288698, 30, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 222, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (238.81, 119.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (249.74, 125.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (290, 600, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934) [M::mem_pestat] mean and std.dev: (569.61, 354.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 321, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1921) [M::mem_pestat] mean and std.dev: (394.18, 318.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703796 sequences (100000223 bp) in 2.902 CPU sec, 3.243 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 288244, 16, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 261, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (268.67, 136.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (249.42, 124.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 923, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4357) [M::mem_pestat] mean and std.dev: (766.93, 599.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (326.09, 253.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704132 sequences (100000240 bp) in 2.293 CPU sec, 2.305 real sec... [M::mem_process_seqs] Processed 704324 reads in 208.566 CPU sec, 6.870 real sec sorting block 16, #reads = 1085907 mem = 536881032 peak = 937795584 [M::mem_process_seqs] Processed 703906 reads in 143.066 CPU sec, 4.566 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 288217, 30, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 278, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1630) [M::mem_pestat] mean and std.dev: (387.33, 340.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (247.20, 121.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (511, 991, 1727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4159) [M::mem_pestat] mean and std.dev: (951.08, 732.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5375) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 223, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326) [M::mem_pestat] mean and std.dev: (284.69, 176.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 17, #reads = 1086041 mem = 536880891 peak = 944082944 [M::process] read 704300 sequences (100000074 bp) in 2.967 CPU sec, 3.504 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 288530, 18, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 287, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504) [M::mem_pestat] mean and std.dev: (392.53, 367.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (246.50, 121.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 806, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3395) [M::mem_pestat] mean and std.dev: (793.59, 576.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 209, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (263.98, 153.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703796 reads in 210.477 CPU sec, 6.782 real sec [M::process] read 703100 sequences (100000056 bp) in 2.498 CPU sec, 2.518 real sec... [M::mem_process_seqs] Processed 704132 reads in 148.754 CPU sec, 4.957 real sec sorting block 18, #reads = 1083811 mem = 536881234 peak = 944082944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 288404, 18, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 252, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (280.30, 162.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (246.57, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (132, 1502, 1790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5106) [M::mem_pestat] mean and std.dev: (1049.94, 792.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 341, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (483.79, 414.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702980 sequences (100000147 bp) in 3.016 CPU sec, 3.330 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 287963, 18, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 357, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334) [M::mem_pestat] mean and std.dev: (370.38, 277.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (248.82, 122.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (360, 649, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3273) [M::mem_pestat] mean and std.dev: (639.62, 447.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4244) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 299, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (282.33, 150.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702596 sequences (100000280 bp) in 2.248 CPU sec, 2.252 real sec... [M::mem_process_seqs] Processed 704300 reads in 211.361 CPU sec, 7.001 real sec [M::mem_process_seqs] Processed 703100 reads in 142.369 CPU sec, 4.555 real sec sorting block 19, #reads = 1084456 mem = 536878124 peak = 944082944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 288019, 22, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 320, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1678) [M::mem_pestat] mean and std.dev: (415.67, 296.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (248.50, 121.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 497, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3347) [M::mem_pestat] mean and std.dev: (744.73, 687.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 317, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1897) [M::mem_pestat] mean and std.dev: (427.73, 322.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702756 sequences (100000270 bp) in 3.214 CPU sec, 3.544 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 287731, 29, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 275, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1858) [M::mem_pestat] mean and std.dev: (450.11, 431.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 226, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (250.69, 124.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 869, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5114) [M::mem_pestat] mean and std.dev: (1280.38, 1234.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 322, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551) [M::mem_pestat] mean and std.dev: (355.12, 214.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702980 reads in 203.809 CPU sec, 6.535 real sec [M::process] read 702234 sequences (100000068 bp) in 2.409 CPU sec, 2.413 real sec... sorting block 20, #reads = 1084569 mem = 536878811 peak = 944082944 [M::mem_process_seqs] Processed 702596 reads in 145.709 CPU sec, 4.692 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 287750, 24, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 260, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862) [M::mem_pestat] mean and std.dev: (398.79, 384.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 226, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (250.60, 123.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (180, 555, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2301) [M::mem_pestat] mean and std.dev: (473.55, 350.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 307, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2227) [M::mem_pestat] mean and std.dev: (487.67, 438.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 21, #reads = 1085508 mem = 536878138 peak = 944082944 [M::process] read 702808 sequences (100000201 bp) in 2.960 CPU sec, 3.402 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 287963, 22, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 231, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (219.48, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 227, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (252.18, 125.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (176, 977, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3926) [M::mem_pestat] mean and std.dev: (847.05, 633.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 300, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (285.95, 157.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705028 sequences (100000051 bp) in 2.245 CPU sec, 2.294 real sec... [M::mem_process_seqs] Processed 702756 reads in 208.452 CPU sec, 6.751 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 288115, 23, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 231, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (260.08, 161.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 226, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (251.58, 125.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (294, 494, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2265) [M::mem_pestat] mean and std.dev: (604.57, 419.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 299, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800) [M::mem_pestat] mean and std.dev: (574.87, 585.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708328 sequences (100000204 bp) in 2.493 CPU sec, 2.521 real sec... [M::mem_process_seqs] Processed 702808 reads in 121.788 CPU sec, 3.980 real sec sorting block 22, #reads = 1084694 mem = 536877703 peak = 944082944 [M::mem_process_seqs] Processed 702234 reads in 316.459 CPU sec, 10.242 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 288837, 28, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 234, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1374) [M::mem_pestat] mean and std.dev: (320.11, 222.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (246.93, 123.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (344, 541, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (451.12, 305.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 330, 716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1722) [M::mem_pestat] mean and std.dev: (407.15, 291.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 23, #reads = 1084183 mem = 536878883 peak = 944082944 [M::process] read 707988 sequences (100000069 bp) in 2.933 CPU sec, 3.319 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 289556, 17, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 302, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3766) [M::mem_pestat] mean and std.dev: (621.29, 684.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 214, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (239.58, 119.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (102, 283, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (294.33, 233.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 250, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (341.97, 245.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705028 reads in 235.287 CPU sec, 7.566 real sec [M::mem_process_seqs] Processed 708328 reads in 145.650 CPU sec, 4.688 real sec [M::process] read 707194 sequences (100000101 bp) in 2.634 CPU sec, 2.681 real sec... sorting block 24, #reads = 1084729 mem = 536885666 peak = 944082944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 290284, 21, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (356.36, 245.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 215, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (239.72, 118.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 630, 3157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8903) [M::mem_pestat] mean and std.dev: (1839.33, 2258.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 280, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3312) [M::mem_pestat] mean and std.dev: (701.19, 883.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706252 sequences (100000272 bp) in 2.820 CPU sec, 3.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (103, 289482, 22, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 292, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (306.66, 200.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 218, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (242.35, 120.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 763, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2208) [M::mem_pestat] mean and std.dev: (645.75, 451.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 219, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (264.84, 140.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707988 reads in 209.630 CPU sec, 6.772 real sec [M::process] read 705170 sequences (100000038 bp) in 2.363 CPU sec, 2.389 real sec... [M::mem_process_seqs] Processed 707194 reads in 144.098 CPU sec, 4.796 real sec sorting block 25, #reads = 1086579 mem = 536879465 peak = 944082944 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 288952, 15, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 317, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (543.80, 554.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 220, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (244.54, 121.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (442, 1121, 1863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4705) [M::mem_pestat] mean and std.dev: (1110.15, 854.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 262, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (307.92, 172.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704732 sequences (100000133 bp) in 2.928 CPU sec, 3.335 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 288468, 21, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 283, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (258.10, 102.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (246.82, 122.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 726, 3079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8653) [M::mem_pestat] mean and std.dev: (1305.67, 1208.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 282, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191) [M::mem_pestat] mean and std.dev: (300.06, 148.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704166 sequences (100000164 bp) in 2.424 CPU sec, 2.463 real sec... [M::mem_process_seqs] Processed 706252 reads in 212.354 CPU sec, 7.032 real sec sorting block 26, #reads = 1087240 mem = 536877902 peak = 944082944 [M::mem_process_seqs] Processed 705170 reads in 144.917 CPU sec, 4.736 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 288093, 24, 93) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 225, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (277.59, 170.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 223, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (247.56, 122.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (313, 713, 2014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5416) [M::mem_pestat] mean and std.dev: (967.57, 763.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7117) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 306, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (308.23, 164.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704086 sequences (100000072 bp) in 2.742 CPU sec, 3.121 real sec... sorting block 27, #reads = 1086590 mem = 536879243 peak = 955187200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 287923, 28, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 309, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3003) [M::mem_pestat] mean and std.dev: (581.57, 766.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 225, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (249.16, 123.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 915, 1730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4994) [M::mem_pestat] mean and std.dev: (882.04, 904.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 254, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (266.89, 145.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704200 sequences (100000180 bp) in 2.426 CPU sec, 2.600 real sec... [M::mem_process_seqs] Processed 704732 reads in 206.541 CPU sec, 6.800 real sec [M::mem_process_seqs] Processed 704166 reads in 143.712 CPU sec, 4.772 real sec sorting block 28, #reads = 1084591 mem = 536880895 peak = 955187200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 288284, 26, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 297, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771) [M::mem_pestat] mean and std.dev: (360.81, 304.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 225, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (249.74, 124.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 509, 1726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4624) [M::mem_pestat] mean and std.dev: (956.56, 1119.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 272, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (239.57, 75.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703360 sequences (100000080 bp) in 3.043 CPU sec, 3.295 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 288614, 24, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 211, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (213.88, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (248.52, 123.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (383, 728, 2216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5882) [M::mem_pestat] mean and std.dev: (909.86, 911.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7715) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 301, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1366) [M::mem_pestat] mean and std.dev: (372.13, 254.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703308 sequences (100000103 bp) in 2.360 CPU sec, 2.471 real sec... [M::mem_process_seqs] Processed 704086 reads in 210.165 CPU sec, 6.894 real sec sorting block 29, #reads = 1085075 mem = 536880086 peak = 955187200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 288034, 25, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 342, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412) [M::mem_pestat] mean and std.dev: (358.71, 233.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 223, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (247.56, 121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (136, 684, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2407) [M::mem_pestat] mean and std.dev: (587.70, 528.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 251, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (277.21, 142.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703436 sequences (100000132 bp) in 2.625 CPU sec, 2.707 real sec... [M::mem_process_seqs] Processed 703360 reads in 122.492 CPU sec, 3.989 real sec [M::mem_process_seqs] Processed 704200 reads in 309.929 CPU sec, 10.045 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 287956, 17, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 311, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (314.55, 228.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (247.61, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (442, 654, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2995) [M::mem_pestat] mean and std.dev: (821.12, 611.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 402, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2713) [M::mem_pestat] mean and std.dev: (456.52, 359.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 30, #reads = 1084919 mem = 536879714 peak = 955187200 [M::process] read 703276 sequences (100000009 bp) in 2.839 CPU sec, 3.286 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 288075, 20, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (241.38, 88.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (247.67, 121.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (324, 739, 1261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3135) [M::mem_pestat] mean and std.dev: (672.71, 704.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 212, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (268.48, 155.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703308 reads in 236.771 CPU sec, 7.582 real sec [M::process] read 702860 sequences (100000195 bp) in 2.565 CPU sec, 2.601 real sec... [M::mem_process_seqs] Processed 703436 reads in 148.691 CPU sec, 4.742 real sec sorting block 31, #reads = 1084197 mem = 536884457 peak = 955187200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 287733, 27, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 256, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1237) [M::mem_pestat] mean and std.dev: (311.04, 213.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (248.86, 122.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 480, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3791) [M::mem_pestat] mean and std.dev: (947.58, 1000.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1720) [M::mem_pestat] mean and std.dev: (394.89, 373.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702978 sequences (100000189 bp) in 2.806 CPU sec, 3.386 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 288291, 19, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 287, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (305.50, 165.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (249.09, 122.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 260, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (313.38, 277.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2729) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 291, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (311.10, 153.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703230 sequences (100000070 bp) in 2.176 CPU sec, 2.190 real sec... [M::mem_process_seqs] Processed 703276 reads in 211.655 CPU sec, 7.028 real sec sorting block 32, #reads = 1082516 mem = 536881661 peak = 955187200 [M::mem_process_seqs] Processed 702860 reads in 147.747 CPU sec, 4.756 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 287761, 21, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 280, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (296.38, 178.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (249.96, 123.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (419, 832, 2140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5582) [M::mem_pestat] mean and std.dev: (1499.81, 1574.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 278, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240) [M::mem_pestat] mean and std.dev: (329.54, 224.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703234 sequences (100000238 bp) in 2.942 CPU sec, 3.201 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 288115, 25, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 248, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177) [M::mem_pestat] mean and std.dev: (275.45, 184.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (249.75, 123.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 502, 2647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7607) [M::mem_pestat] mean and std.dev: (1105.21, 1472.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10087) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 239, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (304.83, 202.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703230 sequences (100000004 bp) in 2.388 CPU sec, 2.389 real sec... [M::mem_process_seqs] Processed 702978 reads in 210.649 CPU sec, 6.788 real sec sorting block 33, #reads = 1082995 mem = 536879348 peak = 955187200 [M::mem_process_seqs] Processed 703230 reads in 145.202 CPU sec, 4.853 real sec sorting block 34, #reads = 1083573 mem = 536881565 peak = 955187200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 287911, 36, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 240, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1335) [M::mem_pestat] mean and std.dev: (360.22, 295.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (249.42, 123.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 961, 2440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6804) [M::mem_pestat] mean and std.dev: (1282.50, 1373.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 350, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1632) [M::mem_pestat] mean and std.dev: (423.59, 359.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706944 sequences (100000115 bp) in 3.158 CPU sec, 3.466 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 287913, 15, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 225, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (364.68, 359.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (248.94, 123.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (183, 462, 2221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6297) [M::mem_pestat] mean and std.dev: (870.40, 915.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 255, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (276.82, 133.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707236 sequences (100000106 bp) in 2.341 CPU sec, 2.441 real sec... [M::mem_process_seqs] Processed 703234 reads in 212.952 CPU sec, 7.128 real sec [M::mem_process_seqs] Processed 703230 reads in 146.853 CPU sec, 4.848 real sec sorting block 35, #reads = 1082935 mem = 536885537 peak = 955187200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 288734, 19, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 236, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (284.83, 193.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 218, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (242.05, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 397, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4203) [M::mem_pestat] mean and std.dev: (691.24, 766.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 291, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3312) [M::mem_pestat] mean and std.dev: (649.76, 791.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706628 sequences (100000257 bp) in 3.029 CPU sec, 3.280 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 289043, 26, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 289, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1255) [M::mem_pestat] mean and std.dev: (385.77, 285.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 218, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (241.98, 119.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (503, 902, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4091) [M::mem_pestat] mean and std.dev: (1109.72, 807.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 220, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (219.52, 112.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706210 sequences (100000227 bp) in 2.323 CPU sec, 2.330 real sec... [M::mem_process_seqs] Processed 706944 reads in 211.874 CPU sec, 6.894 real sec [M::mem_process_seqs] Processed 707236 reads in 141.946 CPU sec, 4.539 real sec sorting block 36, #reads = 1081612 mem = 536879959 peak = 955187200 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 288522, 24, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 310, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570) [M::mem_pestat] mean and std.dev: (279.57, 190.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (242.89, 120.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 97, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (229.13, 244.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 365, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (465.44, 371.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705902 sequences (100000277 bp) in 3.065 CPU sec, 3.404 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 289060, 16, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 257, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (240.84, 95.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 220, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (244.37, 121.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (395, 1355, 1714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4352) [M::mem_pestat] mean and std.dev: (761.38, 628.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 305, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418) [M::mem_pestat] mean and std.dev: (348.84, 194.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706628 reads in 203.239 CPU sec, 6.562 real sec [M::process] read 705396 sequences (100000252 bp) in 2.292 CPU sec, 2.303 real sec... sorting block 37, #reads = 1087552 mem = 536881228 peak = 955187200 [M::mem_process_seqs] Processed 706210 reads in 144.441 CPU sec, 4.611 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 288943, 16, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 298, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1890) [M::mem_pestat] mean and std.dev: (448.14, 432.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 221, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (245.82, 122.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (381, 1372, 2596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7026) [M::mem_pestat] mean and std.dev: (1562.62, 1397.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 347, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2598) [M::mem_pestat] mean and std.dev: (531.53, 502.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 38, #reads = 1087064 mem = 536880766 peak = 981331968 [M::process] read 704830 sequences (100000297 bp) in 3.034 CPU sec, 3.397 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 289163, 35, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 209, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (229.58, 109.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 222, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (247.26, 123.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 863, 1863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5371) [M::mem_pestat] mean and std.dev: (955.71, 1025.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7125) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 224, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (337.64, 251.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705902 reads in 203.863 CPU sec, 6.566 real sec [M::process] read 704456 sequences (100000164 bp) in 2.397 CPU sec, 2.434 real sec... [M::mem_process_seqs] Processed 705396 reads in 142.514 CPU sec, 4.649 real sec sorting block 39, #reads = 1086780 mem = 536879382 peak = 981331968 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 288686, 26, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 287, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1826) [M::mem_pestat] mean and std.dev: (392.59, 352.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 224, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (248.45, 123.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (176, 593, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907) [M::mem_pestat] mean and std.dev: (593.67, 506.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 221, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (312.54, 216.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704670 sequences (100000110 bp) in 2.804 CPU sec, 3.151 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 287983, 17, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 286, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (336.06, 254.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (248.67, 123.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 748, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5273) [M::mem_pestat] mean and std.dev: (988.94, 931.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 265, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348) [M::mem_pestat] mean and std.dev: (293.17, 208.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1738) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705812 sequences (100000031 bp) in 2.371 CPU sec, 2.392 real sec... [M::mem_process_seqs] Processed 704830 reads in 209.468 CPU sec, 6.828 real sec [M::mem_process_seqs] Processed 704456 reads in 147.708 CPU sec, 4.743 real sec sorting block 40, #reads = 1085858 mem = 536878226 peak = 981331968 [M::process] read 704638 sequences (100000111 bp) in 2.875 CPU sec, 3.214 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 288846, 24, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 199, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (218.06, 128.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 217, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (242.21, 119.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 1286, 1967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5125) [M::mem_pestat] mean and std.dev: (1059.04, 798.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 272, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (262.35, 161.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704302 sequences (100000273 bp) in 2.347 CPU sec, 2.351 real sec... [M::mem_process_seqs] Processed 705812 reads in 119.601 CPU sec, 3.775 real sec sorting block 41, #reads = 1083981 mem = 536880365 peak = 981331968 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 288293, 18, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 267, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469) [M::mem_pestat] mean and std.dev: (385.25, 329.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 223, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (248.38, 123.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (334, 544, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1576) [M::mem_pestat] mean and std.dev: (575.94, 354.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1992) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 297, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2121) [M::mem_pestat] mean and std.dev: (457.25, 503.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 288550, 37, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 196, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (240.49, 162.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (244.75, 120.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (681, 1161, 1908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4362) [M::mem_pestat] mean and std.dev: (1215.74, 831.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 355, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2569) [M::mem_pestat] mean and std.dev: (561.84, 534.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704670 reads in 325.304 CPU sec, 10.401 real sec [M::process] read 703568 sequences (100000003 bp) in 2.534 CPU sec, 2.599 real sec... [M::mem_process_seqs] Processed 704638 reads in 174.478 CPU sec, 5.796 real sec sorting block 42, #reads = 1084675 mem = 536877105 peak = 981331968 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 288290, 27, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 209, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (205.78, 78.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (244.96, 119.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (393, 628, 1460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3594) [M::mem_pestat] mean and std.dev: (761.38, 640.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4661) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 295, 757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949) [M::mem_pestat] mean and std.dev: (416.22, 387.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703486 sequences (100000184 bp) in 3.209 CPU sec, 3.677 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 287988, 34, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 301, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419) [M::mem_pestat] mean and std.dev: (302.98, 174.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 223, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (246.53, 120.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (254, 779, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3593) [M::mem_pestat] mean and std.dev: (965.91, 782.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 367, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1506) [M::mem_pestat] mean and std.dev: (357.76, 243.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703090 sequences (100000090 bp) in 2.402 CPU sec, 2.405 real sec... [M::mem_process_seqs] Processed 704302 reads in 214.976 CPU sec, 7.131 real sec sorting block 43, #reads = 1084730 mem = 536878773 peak = 981331968 [M::mem_process_seqs] Processed 703568 reads in 148.214 CPU sec, 4.831 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 288210, 20, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 239, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (257.94, 150.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 223, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (245.70, 119.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 344, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1875) [M::mem_pestat] mean and std.dev: (435.72, 429.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 317, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1607) [M::mem_pestat] mean and std.dev: (372.00, 278.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 44, #reads = 1083868 mem = 536881641 peak = 997990400 [M::process] read 702656 sequences (100000120 bp) in 3.090 CPU sec, 3.458 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 287969, 18, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 255, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (251.82, 117.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (246.55, 119.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 413, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1979) [M::mem_pestat] mean and std.dev: (394.53, 272.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 249, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (323.81, 224.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703180 sequences (100000047 bp) in 2.481 CPU sec, 2.484 real sec... [M::mem_process_seqs] Processed 703486 reads in 211.716 CPU sec, 6.866 real sec [M::mem_process_seqs] Processed 703090 reads in 143.801 CPU sec, 4.568 real sec sorting block 45, #reads = 1084008 mem = 536883040 peak = 997990400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 287790, 27, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 280, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (275.87, 147.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (248.26, 120.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (316, 686, 1965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5263) [M::mem_pestat] mean and std.dev: (807.09, 727.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 293, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (318.00, 186.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702800 sequences (100000171 bp) in 3.071 CPU sec, 3.538 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 287932, 23, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 307, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (340.54, 210.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (248.68, 122.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 524, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1655) [M::mem_pestat] mean and std.dev: (492.10, 336.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 283, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1182) [M::mem_pestat] mean and std.dev: (308.20, 207.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702656 reads in 213.692 CPU sec, 6.870 real sec [M::process] read 703764 sequences (100000105 bp) in 2.471 CPU sec, 2.478 real sec... [M::mem_process_seqs] Processed 703180 reads in 149.845 CPU sec, 4.795 real sec sorting block 46, #reads = 1083604 mem = 536880809 peak = 997990400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 287697, 28, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 313, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (299.10, 173.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (249.13, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (359, 740, 2168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5786) [M::mem_pestat] mean and std.dev: (973.76, 913.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7595) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 314, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1793) [M::mem_pestat] mean and std.dev: (357.37, 230.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 712644 sequences (100000145 bp) in 2.951 CPU sec, 3.368 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 288304, 18, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 215, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (234.78, 123.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (247.58, 122.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 559, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2323) [M::mem_pestat] mean and std.dev: (536.53, 348.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 318, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (332.53, 232.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702800 reads in 209.186 CPU sec, 6.814 real sec [M::process] read 710396 sequences (100000105 bp) in 2.366 CPU sec, 2.410 real sec... sorting block 47, #reads = 1082924 mem = 536879101 peak = 997990400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 291466, 16, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 262, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (255.12, 124.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (225.31, 109.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (327, 386, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1911) [M::mem_pestat] mean and std.dev: (507.20, 275.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2439) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 284, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913) [M::mem_pestat] mean and std.dev: (402.49, 342.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708260 sequences (100000076 bp) in 2.589 CPU sec, 2.622 real sec... [M::mem_process_seqs] Processed 712644 reads in 122.219 CPU sec, 3.931 real sec sorting block 48, #reads = 1085132 mem = 536880320 peak = 997990400 [M::mem_process_seqs] Processed 703764 reads in 319.994 CPU sec, 10.320 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 290830, 23, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 207, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (228.88, 132.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (233.03, 115.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (437, 437, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2636) [M::mem_pestat] mean and std.dev: (689.78, 480.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 266, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (278.80, 156.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707340 sequences (100000022 bp) in 3.135 CPU sec, 3.712 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 290248, 28, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 272, 1522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4260) [M::mem_pestat] mean and std.dev: (623.48, 706.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (237.57, 118.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (747, 1609, 3396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8694) [M::mem_pestat] mean and std.dev: (1921.43, 1407.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 228, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (251.00, 164.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 710396 reads in 231.468 CPU sec, 7.476 real sec [M::mem_process_seqs] Processed 708260 reads in 142.615 CPU sec, 4.560 real sec sorting block 49, #reads = 1083510 mem = 536879225 peak = 997990400 [M::process] read 706728 sequences (100000097 bp) in 2.770 CPU sec, 2.897 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 289887, 21, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 286, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (269.62, 117.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 215, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (240.43, 119.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (175, 352, 1927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5431) [M::mem_pestat] mean and std.dev: (841.38, 877.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 259, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (298.37, 160.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706262 sequences (100000205 bp) in 2.790 CPU sec, 3.287 real sec... sorting block 50, #reads = 1087742 mem = 536881462 peak = 997990400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 289492, 22, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 309, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (430.62, 393.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 217, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (242.63, 121.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (328, 666, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4585) [M::mem_pestat] mean and std.dev: (923.76, 881.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6004) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 307, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1911) [M::mem_pestat] mean and std.dev: (330.95, 266.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707340 reads in 203.605 CPU sec, 6.615 real sec [M::mem_process_seqs] Processed 706728 reads in 144.869 CPU sec, 4.668 real sec [M::process] read 705964 sequences (100000060 bp) in 2.567 CPU sec, 2.692 real sec... sorting block 51, #reads = 1085929 mem = 536877978 peak = 997990400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 289145, 27, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 263, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153) [M::mem_pestat] mean and std.dev: (453.34, 378.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (243.54, 121.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (494, 1263, 1950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4862) [M::mem_pestat] mean and std.dev: (1201.83, 1020.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 303, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (358.33, 262.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705282 sequences (100000161 bp) in 2.966 CPU sec, 3.328 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 289163, 29, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 263, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2107) [M::mem_pestat] mean and std.dev: (356.95, 386.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (244.47, 122.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 874, 1440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3572) [M::mem_pestat] mean and std.dev: (943.59, 801.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4638) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 285, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (306.65, 150.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706262 reads in 204.711 CPU sec, 6.600 real sec [M::process] read 704542 sequences (100000265 bp) in 2.288 CPU sec, 2.349 real sec... [M::mem_process_seqs] Processed 705964 reads in 144.287 CPU sec, 4.657 real sec sorting block 52, #reads = 1085891 mem = 536878078 peak = 997990400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288597, 11, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 256, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1587) [M::mem_pestat] mean and std.dev: (367.90, 293.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (245.59, 122.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (716, 932, 4445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11903) [M::mem_pestat] mean and std.dev: (2580.36, 3284.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 224, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (281.50, 187.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704332 sequences (100000053 bp) in 2.842 CPU sec, 3.405 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 288463, 16, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 279, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (298.13, 194.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (246.55, 122.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 277, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3556) [M::mem_pestat] mean and std.dev: (606.53, 630.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4715) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 299, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (326.94, 172.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705282 reads in 209.254 CPU sec, 6.806 real sec [M::process] read 704288 sequences (100000197 bp) in 2.475 CPU sec, 2.491 real sec... [M::mem_process_seqs] Processed 704542 reads in 147.608 CPU sec, 4.810 real sec sorting block 53, #reads = 1084734 mem = 536880373 peak = 997990400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 288374, 20, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 292, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (317.17, 230.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (246.13, 122.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (421, 544, 1440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3478) [M::mem_pestat] mean and std.dev: (715.00, 565.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 282, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (313.11, 170.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704034 sequences (100000178 bp) in 3.160 CPU sec, 3.420 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 288146, 17, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 262, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (312.69, 217.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (246.22, 121.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (425, 910, 2327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6131) [M::mem_pestat] mean and std.dev: (1710.53, 1837.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9061) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 341, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (296.88, 126.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703738 sequences (100000295 bp) in 2.388 CPU sec, 2.395 real sec... [M::mem_process_seqs] Processed 704332 reads in 213.470 CPU sec, 6.911 real sec sorting block 54, #reads = 1083169 mem = 536877189 peak = 997990400 [M::mem_process_seqs] Processed 704288 reads in 147.459 CPU sec, 4.753 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 288123, 25, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 282, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (299.87, 187.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (247.04, 122.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (541, 666, 4818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13372) [M::mem_pestat] mean and std.dev: (2111.52, 2589.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 263, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1962) [M::mem_pestat] mean and std.dev: (442.95, 412.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 55, #reads = 1082913 mem = 536880948 peak = 1009836032 [M::process] read 703706 sequences (100000217 bp) in 3.089 CPU sec, 3.301 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 288278, 30, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (239.42, 147.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (246.84, 121.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (85, 368, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4408) [M::mem_pestat] mean and std.dev: (507.79, 672.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 265, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671) [M::mem_pestat] mean and std.dev: (397.31, 333.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703526 sequences (100000118 bp) in 2.430 CPU sec, 2.442 real sec... [M::mem_process_seqs] Processed 704034 reads in 215.818 CPU sec, 6.977 real sec [M::mem_process_seqs] Processed 703738 reads in 148.137 CPU sec, 4.782 real sec sorting block 56, #reads = 1081815 mem = 536884339 peak = 1009836032 [M::process] read 703828 sequences (100000189 bp) in 3.116 CPU sec, 3.585 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 287877, 24, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 407, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (401.72, 279.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 223, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (247.57, 121.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 1147, 5529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15791) [M::mem_pestat] mean and std.dev: (2943.17, 3268.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 348, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (316.37, 158.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703526 reads in 119.786 CPU sec, 3.821 real sec [M::process] read 703180 sequences (100000292 bp) in 2.530 CPU sec, 2.546 real sec... sorting block 57, #reads = 1082574 mem = 536878013 peak = 1009836032 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 288173, 18, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 320, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (306.80, 158.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (247.08, 122.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 597, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2516) [M::mem_pestat] mean and std.dev: (515.60, 496.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 295, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (312.91, 158.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 288043, 39, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 300, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844) [M::mem_pestat] mean and std.dev: (426.34, 394.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (247.45, 122.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (102, 166, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1530) [M::mem_pestat] mean and std.dev: (308.36, 273.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2006) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 266, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2308) [M::mem_pestat] mean and std.dev: (403.73, 461.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703706 reads in 331.950 CPU sec, 10.784 real sec [M::process] read 706404 sequences (100000071 bp) in 2.839 CPU sec, 2.921 real sec... [M::mem_process_seqs] Processed 703828 reads in 176.915 CPU sec, 5.691 real sec sorting block 58, #reads = 1082597 mem = 536881592 peak = 1009836032 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 288170, 31, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2051) [M::mem_pestat] mean and std.dev: (395.29, 378.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (249.01, 123.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (236, 1165, 2864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8120) [M::mem_pestat] mean and std.dev: (1754.26, 1951.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 336, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478) [M::mem_pestat] mean and std.dev: (417.73, 262.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 714860 sequences (100000213 bp) in 2.895 CPU sec, 3.125 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 289360, 13, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 275, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2142) [M::mem_pestat] mean and std.dev: (450.51, 427.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 217, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (241.89, 120.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 613, 1205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2899) [M::mem_pestat] mean and std.dev: (592.73, 365.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 324, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (294.90, 122.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 713678 sequences (100000052 bp) in 2.406 CPU sec, 2.413 real sec... [M::mem_process_seqs] Processed 703180 reads in 217.860 CPU sec, 7.136 real sec [M::mem_process_seqs] Processed 706404 reads in 156.358 CPU sec, 4.998 real sec sorting block 59, #reads = 1080602 mem = 536884375 peak = 1009836032 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 292448, 18, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 205, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (209.52, 117.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 199, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (220.88, 106.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 1121, 1232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3138) [M::mem_pestat] mean and std.dev: (732.69, 535.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4091) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 276, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (313.45, 182.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 711020 sequences (100000017 bp) in 2.788 CPU sec, 3.053 real sec... [M::process] read 710688 sequences (100000074 bp) in 2.468 CPU sec, 2.506 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 291637, 17, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 262, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2182) [M::mem_pestat] mean and std.dev: (473.18, 540.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (223.86, 108.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (480, 682, 1246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2778) [M::mem_pestat] mean and std.dev: (682.07, 389.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 257, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (257.90, 150.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 714860 reads in 226.829 CPU sec, 7.281 real sec sorting block 60, #reads = 1082155 mem = 536888457 peak = 1024360448 [M::mem_process_seqs] Processed 713678 reads in 157.641 CPU sec, 5.060 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 291157, 24, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 233, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (327.98, 233.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (230.74, 113.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 989, 3431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9777) [M::mem_pestat] mean and std.dev: (1854.62, 2099.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 235, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (302.58, 218.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 61, #reads = 1084516 mem = 536884467 peak = 1037783040 [M::process] read 709310 sequences (100000296 bp) in 3.159 CPU sec, 3.558 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 291299, 19, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 236, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (292.72, 210.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (231.89, 114.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 670, 1605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4389) [M::mem_pestat] mean and std.dev: (835.78, 741.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5781) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 258, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (260.71, 115.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 709308 sequences (100000164 bp) in 2.433 CPU sec, 2.453 real sec... [M::mem_process_seqs] Processed 711020 reads in 221.471 CPU sec, 7.179 real sec [M::mem_process_seqs] Processed 710688 reads in 154.134 CPU sec, 5.088 real sec sorting block 62, #reads = 1085198 mem = 536879179 peak = 1037783040 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 291038, 21, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 324, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1930) [M::mem_pestat] mean and std.dev: (490.85, 446.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (235.39, 116.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 494, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2916) [M::mem_pestat] mean and std.dev: (778.10, 671.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 295, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2707) [M::mem_pestat] mean and std.dev: (428.43, 350.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708778 sequences (100000106 bp) in 2.990 CPU sec, 3.270 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 290674, 21, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 368, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (355.56, 241.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (235.82, 116.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (369, 938, 938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2076) [M::mem_pestat] mean and std.dev: (736.15, 380.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 241, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (340.73, 268.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707544 sequences (100000121 bp) in 2.441 CPU sec, 2.452 real sec... [M::mem_process_seqs] Processed 709310 reads in 219.079 CPU sec, 7.177 real sec [M::mem_process_seqs] Processed 709308 reads in 149.584 CPU sec, 4.894 real sec sorting block 63, #reads = 1084172 mem = 536880152 peak = 1037783040 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 290779, 11, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 294, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217) [M::mem_pestat] mean and std.dev: (312.75, 174.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 213, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (238.67, 118.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 455, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2324) [M::mem_pestat] mean and std.dev: (386.67, 253.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 291, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (318.92, 195.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706516 sequences (100000236 bp) in 3.216 CPU sec, 3.622 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 289762, 17, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 287, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2435) [M::mem_pestat] mean and std.dev: (485.54, 594.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 215, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (240.93, 120.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 357, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2651) [M::mem_pestat] mean and std.dev: (608.06, 542.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 290, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (286.25, 138.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 708778 reads in 216.706 CPU sec, 7.086 real sec [M::process] read 705614 sequences (100000080 bp) in 2.501 CPU sec, 2.514 real sec... sorting block 64, #reads = 1084128 mem = 536878899 peak = 1037783040 [M::mem_process_seqs] Processed 707544 reads in 149.251 CPU sec, 4.804 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 289091, 8, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 235, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (276.54, 160.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 217, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (242.43, 120.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 343, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267) [M::mem_pestat] mean and std.dev: (345.83, 216.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 65, #reads = 1083961 mem = 536885071 peak = 1054437376 [M::process] read 705094 sequences (100000293 bp) in 3.274 CPU sec, 3.699 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 288710, 28, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3153) [M::mem_pestat] mean and std.dev: (524.67, 514.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (243.81, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (459, 739, 2878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7716) [M::mem_pestat] mean and std.dev: (1632.41, 2009.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 274, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (294.49, 147.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706516 reads in 220.343 CPU sec, 7.141 real sec [M::process] read 704648 sequences (100000275 bp) in 2.460 CPU sec, 2.512 real sec... [M::mem_process_seqs] Processed 705614 reads in 154.593 CPU sec, 5.152 real sec sorting block 66, #reads = 1083579 mem = 536883986 peak = 1054437376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 288457, 32, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 254, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (294.73, 218.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (244.14, 120.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 539, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3413) [M::mem_pestat] mean and std.dev: (838.55, 861.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 253, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (239.29, 116.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704612 sequences (100000032 bp) in 3.022 CPU sec, 3.601 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 288350, 16, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 307, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1534) [M::mem_pestat] mean and std.dev: (370.51, 313.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (246.22, 122.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (163, 464, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2650) [M::mem_pestat] mean and std.dev: (510.69, 451.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 257, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001) [M::mem_pestat] mean and std.dev: (299.01, 193.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704512 sequences (100000128 bp) in 2.571 CPU sec, 2.573 real sec... [M::mem_process_seqs] Processed 705094 reads in 222.565 CPU sec, 7.375 real sec [M::mem_process_seqs] Processed 704648 reads in 152.665 CPU sec, 5.000 real sec sorting block 67, #reads = 1081607 mem = 536881221 peak = 1054437376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 289094, 18, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 319, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1517) [M::mem_pestat] mean and std.dev: (409.00, 304.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (245.63, 121.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (187, 391, 1893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5305) [M::mem_pestat] mean and std.dev: (876.89, 953.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 252, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (275.49, 129.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702926 sequences (100000186 bp) in 2.892 CPU sec, 3.313 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 288847, 34, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 251, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (220.07, 91.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (245.96, 121.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 459, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2487) [M::mem_pestat] mean and std.dev: (573.45, 515.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (222.30, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703708 sequences (100000103 bp) in 2.480 CPU sec, 2.487 real sec... [M::mem_process_seqs] Processed 704612 reads in 215.837 CPU sec, 7.066 real sec sorting block 68, #reads = 1081150 mem = 536880086 peak = 1054437376 [M::mem_process_seqs] Processed 704512 reads in 149.964 CPU sec, 4.789 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 287841, 17, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 294, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2291) [M::mem_pestat] mean and std.dev: (437.57, 435.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (249.17, 123.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 1366, 3108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9022) [M::mem_pestat] mean and std.dev: (1523.82, 1216.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11979) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 318, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1919) [M::mem_pestat] mean and std.dev: (468.22, 440.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 69, #reads = 1082644 mem = 536879880 peak = 1054437376 [M::process] read 704942 sequences (100000112 bp) in 3.005 CPU sec, 3.372 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 288525, 15, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 203, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (317.94, 282.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (248.32, 123.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 1332, 1593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4031) [M::mem_pestat] mean and std.dev: (985.71, 737.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 244, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1293) [M::mem_pestat] mean and std.dev: (324.85, 227.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 712812 sequences (100000297 bp) in 2.470 CPU sec, 2.500 real sec... [M::mem_process_seqs] Processed 702926 reads in 204.194 CPU sec, 6.590 real sec [M::mem_process_seqs] Processed 703708 reads in 145.461 CPU sec, 4.696 real sec sorting block 70, #reads = 1082378 mem = 536877353 peak = 1054437376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 288169, 19, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 285, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (283.57, 130.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (244.42, 121.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (464, 682, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2738) [M::mem_pestat] mean and std.dev: (723.44, 594.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 314, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204) [M::mem_pestat] mean and std.dev: (352.84, 217.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 710922 sequences (100000012 bp) in 3.051 CPU sec, 3.314 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 292065, 12, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 259, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1856) [M::mem_pestat] mean and std.dev: (366.95, 359.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (226.59, 110.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (288, 461, 4026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11502) [M::mem_pestat] mean and std.dev: (1740.67, 2802.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 273, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (321.22, 207.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 709710 sequences (100000237 bp) in 2.430 CPU sec, 2.445 real sec... [M::mem_process_seqs] Processed 704942 reads in 227.118 CPU sec, 7.388 real sec [M::mem_process_seqs] Processed 712812 reads in 153.238 CPU sec, 5.076 real sec sorting block 71, #reads = 1079019 mem = 536876253 peak = 1054437376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 290823, 23, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 226, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (259.09, 162.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (231.22, 113.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 610, 1328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3196) [M::mem_pestat] mean and std.dev: (540.61, 322.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 358, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1642) [M::mem_pestat] mean and std.dev: (421.29, 297.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708222 sequences (100000267 bp) in 3.061 CPU sec, 3.448 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 291032, 24, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 220, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242) [M::mem_pestat] mean and std.dev: (278.89, 187.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (234.58, 115.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 457, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2714) [M::mem_pestat] mean and std.dev: (598.54, 531.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 365, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1778) [M::mem_pestat] mean and std.dev: (443.19, 390.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2316) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 710922 reads in 206.135 CPU sec, 6.815 real sec [M::process] read 707710 sequences (100000161 bp) in 2.499 CPU sec, 2.587 real sec... sorting block 72, #reads = 1086924 mem = 536880774 peak = 1054437376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 290419, 23, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2007) [M::mem_pestat] mean and std.dev: (447.77, 432.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 213, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (237.74, 117.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 407, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2322) [M::mem_pestat] mean and std.dev: (488.29, 359.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2992) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 208, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (221.84, 126.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 708222 reads in 114.161 CPU sec, 3.698 real sec [M::process] read 707472 sequences (100000291 bp) in 2.808 CPU sec, 2.954 real sec... sorting block 73, #reads = 1087835 mem = 536881354 peak = 1054437376 [M::mem_process_seqs] Processed 709710 reads in 312.235 CPU sec, 10.132 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290229, 17, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 431, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3417) [M::mem_pestat] mean and std.dev: (692.06, 677.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 215, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (239.39, 117.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (133, 532, 4741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13957) [M::mem_pestat] mean and std.dev: (1835.76, 2702.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (407.93, 297.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707856 sequences (100000285 bp) in 3.187 CPU sec, 3.857 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 290641, 22, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 227, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1838) [M::mem_pestat] mean and std.dev: (416.87, 411.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 216, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (240.90, 119.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (369, 512, 1205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2877) [M::mem_pestat] mean and std.dev: (562.61, 317.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 260, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (275.80, 157.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707710 reads in 237.067 CPU sec, 7.658 real sec [M::mem_process_seqs] Processed 707472 reads in 154.223 CPU sec, 4.989 real sec sorting block 74, #reads = 1087949 mem = 536879643 peak = 1054437376 [M::process] read 706880 sequences (100000185 bp) in 2.951 CPU sec, 3.085 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 290431, 18, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 262, 1065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2905) [M::mem_pestat] mean and std.dev: (432.72, 491.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 215, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (240.24, 119.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (123, 472, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3666) [M::mem_pestat] mean and std.dev: (978.78, 1173.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 316, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631) [M::mem_pestat] mean and std.dev: (425.92, 327.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706484 sequences (100000025 bp) in 3.034 CPU sec, 3.474 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 289950, 12, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 212, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (260.24, 197.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 217, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (241.79, 119.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (425, 839, 1312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3086) [M::mem_pestat] mean and std.dev: (703.09, 445.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 258, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001) [M::mem_pestat] mean and std.dev: (289.88, 147.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707856 reads in 203.894 CPU sec, 6.581 real sec sorting block 75, #reads = 1085666 mem = 536879141 peak = 1054437376 [M::mem_process_seqs] Processed 706880 reads in 140.798 CPU sec, 4.535 real sec [M::process] read 707040 sequences (100000027 bp) in 2.692 CPU sec, 2.817 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 289804, 17, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 273, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2942) [M::mem_pestat] mean and std.dev: (523.85, 698.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 219, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (242.85, 119.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 531, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2209) [M::mem_pestat] mean and std.dev: (419.50, 353.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2927) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 294, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (316.89, 155.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 76, #reads = 1087693 mem = 536878100 peak = 1064804352 [M::process] read 705890 sequences (100000110 bp) in 3.169 CPU sec, 3.463 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 289657, 25, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 262, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (319.82, 244.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 216, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (239.81, 117.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 269, 1412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3898) [M::mem_pestat] mean and std.dev: (683.42, 811.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5141) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 231, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (232.47, 103.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706484 reads in 205.712 CPU sec, 6.755 real sec [M::process] read 705904 sequences (100000233 bp) in 2.628 CPU sec, 2.690 real sec... [M::mem_process_seqs] Processed 707040 reads in 148.200 CPU sec, 4.850 real sec sorting block 77, #reads = 1088125 mem = 536882053 peak = 1064804352 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 289102, 22, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 294, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (272.74, 157.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 219, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (244.42, 121.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 260, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2627) [M::mem_pestat] mean and std.dev: (382.67, 377.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 289, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (284.34, 131.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705160 sequences (100000048 bp) in 3.093 CPU sec, 3.515 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 289321, 19, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 240, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (250.26, 136.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (243.81, 120.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (115, 883, 2154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6232) [M::mem_pestat] mean and std.dev: (1702.11, 1945.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 287, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (301.73, 157.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705890 reads in 211.927 CPU sec, 6.911 real sec [M::process] read 705258 sequences (100000153 bp) in 2.414 CPU sec, 2.441 real sec... [M::mem_process_seqs] Processed 705904 reads in 156.879 CPU sec, 5.099 real sec sorting block 78, #reads = 1084271 mem = 536881936 peak = 1064804352 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 289071, 26, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 218, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (265.08, 168.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (244.45, 120.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (216, 676, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1812) [M::mem_pestat] mean and std.dev: (584.29, 384.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 264, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (332.88, 211.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704620 sequences (100000041 bp) in 3.030 CPU sec, 3.450 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 288964, 27, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (302.77, 193.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (242.68, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 168, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1643) [M::mem_pestat] mean and std.dev: (190.14, 192.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 352, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1703) [M::mem_pestat] mean and std.dev: (456.74, 385.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704866 sequences (100000263 bp) in 2.471 CPU sec, 2.475 real sec... [M::mem_process_seqs] Processed 705160 reads in 216.496 CPU sec, 6.969 real sec sorting block 79, #reads = 1083414 mem = 536879176 peak = 1064804352 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288119, 15, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 269, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (263.40, 164.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (244.94, 120.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (756, 1234, 1896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4176) [M::mem_pestat] mean and std.dev: (1459.07, 1260.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 379, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413) [M::mem_pestat] mean and std.dev: (411.78, 299.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704470 sequences (100000141 bp) in 2.622 CPU sec, 2.741 real sec... [M::mem_process_seqs] Processed 704620 reads in 127.368 CPU sec, 4.292 real sec sorting block 80, #reads = 1082150 mem = 536879271 peak = 1064804352 [M::mem_process_seqs] Processed 705258 reads in 330.637 CPU sec, 10.848 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 288410, 18, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 283, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1926) [M::mem_pestat] mean and std.dev: (392.51, 352.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (244.75, 121.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 310, 1714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4716) [M::mem_pestat] mean and std.dev: (770.82, 852.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 255, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (290.02, 187.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705124 sequences (100000298 bp) in 2.781 CPU sec, 3.560 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 288707, 22, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 246, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855) [M::mem_pestat] mean and std.dev: (361.48, 339.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (245.30, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 415, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (364.62, 209.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 279, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1544) [M::mem_pestat] mean and std.dev: (400.12, 331.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704866 reads in 253.697 CPU sec, 8.151 real sec sorting block 81, #reads = 1081562 mem = 536882176 peak = 1064804352 [M::process] read 716960 sequences (100000010 bp) in 2.730 CPU sec, 2.778 real sec... [M::mem_process_seqs] Processed 704470 reads in 159.019 CPU sec, 5.078 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 288820, 28, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 227, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (289.67, 184.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (244.52, 120.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 879, 2633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6703) [M::mem_pestat] mean and std.dev: (1333.37, 1090.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8738) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 341, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832) [M::mem_pestat] mean and std.dev: (418.95, 419.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 82, #reads = 1080172 mem = 536880750 peak = 1079595008 [M::process] read 714354 sequences (100000050 bp) in 3.325 CPU sec, 3.545 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 293104, 15, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 222, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (297.36, 221.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 195, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (217.01, 104.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 380, 1361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (707.87, 707.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 331, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1421) [M::mem_pestat] mean and std.dev: (347.07, 234.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 713202 sequences (100000090 bp) in 2.385 CPU sec, 2.391 real sec... [M::mem_process_seqs] Processed 705124 reads in 245.883 CPU sec, 7.916 real sec [M::mem_process_seqs] Processed 716960 reads in 167.071 CPU sec, 5.374 real sec sorting block 83, #reads = 1075033 mem = 536880175 peak = 1079595008 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 291813, 20, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 270, 774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2028) [M::mem_pestat] mean and std.dev: (406.46, 448.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (223.96, 108.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (408, 863, 2723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7353) [M::mem_pestat] mean and std.dev: (1566.05, 1426.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 254, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1644) [M::mem_pestat] mean and std.dev: (349.30, 276.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 711352 sequences (100000064 bp) in 3.221 CPU sec, 3.681 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 291704, 12, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 223, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (230.16, 119.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (226.99, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (541, 589, 2474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6340) [M::mem_pestat] mean and std.dev: (1443.25, 1662.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8273) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 297, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795) [M::mem_pestat] mean and std.dev: (349.12, 299.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 710438 sequences (100000110 bp) in 2.420 CPU sec, 2.418 real sec... [M::mem_process_seqs] Processed 714354 reads in 237.742 CPU sec, 7.888 real sec [M::mem_process_seqs] Processed 713202 reads in 158.114 CPU sec, 5.102 real sec sorting block 84, #reads = 1085262 mem = 536876369 peak = 1079595008 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 291249, 20, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 223, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (317.78, 234.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (231.91, 114.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (451, 481, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558) [M::mem_pestat] mean and std.dev: (453.22, 282.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1927) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 243, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117) [M::mem_pestat] mean and std.dev: (315.71, 228.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 709510 sequences (100000223 bp) in 3.347 CPU sec, 3.665 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 290762, 9, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 289, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (313.23, 206.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (233.97, 115.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 284, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (349.09, 245.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 709508 sequences (100000237 bp) in 2.496 CPU sec, 2.512 real sec... [M::mem_process_seqs] Processed 711352 reads in 234.756 CPU sec, 7.598 real sec sorting block 85, #reads = 1083292 mem = 536881595 peak = 1079595008 [M::mem_process_seqs] Processed 710438 reads in 160.312 CPU sec, 5.206 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 290443, 23, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 245, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (244.87, 143.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 212, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (237.23, 118.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (483, 686, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854) [M::mem_pestat] mean and std.dev: (652.45, 320.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 356, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142) [M::mem_pestat] mean and std.dev: (380.27, 207.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 86, #reads = 1083067 mem = 536878535 peak = 1091354624 [M::process] read 708576 sequences (100000194 bp) in 3.042 CPU sec, 3.565 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 290244, 27, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 285, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192) [M::mem_pestat] mean and std.dev: (288.64, 163.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (236.12, 117.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (257, 1177, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7289) [M::mem_pestat] mean and std.dev: (1320.35, 1165.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 325, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613) [M::mem_pestat] mean and std.dev: (490.95, 427.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2203) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708566 sequences (100000258 bp) in 2.639 CPU sec, 2.644 real sec... [M::mem_process_seqs] Processed 709510 reads in 232.362 CPU sec, 7.578 real sec [M::mem_process_seqs] Processed 709508 reads in 162.296 CPU sec, 5.309 real sec sorting block 87, #reads = 1083249 mem = 536885017 peak = 1091354624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 289918, 25, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 273, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (271.90, 142.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 213, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (238.60, 118.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 580, 1364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3658) [M::mem_pestat] mean and std.dev: (506.35, 448.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 305, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570) [M::mem_pestat] mean and std.dev: (388.95, 268.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706428 sequences (100000089 bp) in 3.465 CPU sec, 4.034 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 289626, 22, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 239, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (308.32, 220.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (234.76, 114.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (313, 594, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2803) [M::mem_pestat] mean and std.dev: (692.14, 570.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 249, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (267.31, 137.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 708576 reads in 220.400 CPU sec, 7.230 real sec [M::process] read 705094 sequences (100000203 bp) in 2.597 CPU sec, 2.614 real sec... [M::mem_process_seqs] Processed 708566 reads in 157.167 CPU sec, 5.076 real sec sorting block 88, #reads = 1083336 mem = 536881220 peak = 1091354624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 288785, 21, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (291.04, 190.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (242.45, 119.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 859, 1541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4013) [M::mem_pestat] mean and std.dev: (698.17, 695.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 289, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (316.25, 155.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704576 sequences (100000139 bp) in 3.126 CPU sec, 3.248 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 288042, 22, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 346, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329) [M::mem_pestat] mean and std.dev: (544.73, 549.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (244.52, 120.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (243, 731, 1863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5103) [M::mem_pestat] mean and std.dev: (1008.50, 1037.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 310, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (336.72, 191.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1266) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704052 sequences (100000201 bp) in 2.357 CPU sec, 2.359 real sec... [M::mem_process_seqs] Processed 706428 reads in 217.570 CPU sec, 6.950 real sec sorting block 89, #reads = 1081286 mem = 536881647 peak = 1091354624 [M::mem_process_seqs] Processed 705094 reads in 153.246 CPU sec, 4.891 real sec sorting block 90, #reads = 1080551 mem = 536881105 peak = 1091354624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 288519, 14, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 340, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (318.02, 185.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (244.07, 119.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 402, 4451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13011) [M::mem_pestat] mean and std.dev: (1852.93, 2436.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17291) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 255, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (281.67, 161.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703306 sequences (100000045 bp) in 3.245 CPU sec, 3.388 real sec... [M::process] read 703444 sequences (100000219 bp) in 2.415 CPU sec, 2.417 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 287740, 29, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 258, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3070) [M::mem_pestat] mean and std.dev: (513.61, 482.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (243.54, 118.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 763, 2455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6931) [M::mem_pestat] mean and std.dev: (1385.31, 1624.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 325, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3154) [M::mem_pestat] mean and std.dev: (508.71, 502.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704576 reads in 218.565 CPU sec, 7.032 real sec [M::mem_process_seqs] Processed 704052 reads in 147.997 CPU sec, 4.775 real sec sorting block 91, #reads = 1081165 mem = 536886199 peak = 1091354624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 288191, 20, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 222, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (259.32, 119.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (245.41, 119.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 561, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3437) [M::mem_pestat] mean and std.dev: (661.75, 543.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 271, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424) [M::mem_pestat] mean and std.dev: (352.54, 304.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702688 sequences (100000154 bp) in 3.250 CPU sec, 3.426 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 287638, 23, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 362, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434) [M::mem_pestat] mean and std.dev: (365.47, 257.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (246.70, 120.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 513, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3676) [M::mem_pestat] mean and std.dev: (683.35, 767.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 294, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (331.86, 227.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702830 sequences (100000000 bp) in 2.369 CPU sec, 2.371 real sec... [M::mem_process_seqs] Processed 703306 reads in 212.828 CPU sec, 6.812 real sec [M::mem_process_seqs] Processed 703444 reads in 145.783 CPU sec, 4.740 real sec sorting block 92, #reads = 1081022 mem = 536880381 peak = 1091354624 [M::process] read 282796 sequences (40155251 bp) in 1.429 CPU sec, 1.660 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 287985, 20, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 309, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1490) [M::mem_pestat] mean and std.dev: (407.90, 339.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (248.66, 121.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (490, 1083, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4933) [M::mem_pestat] mean and std.dev: (1012.61, 796.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 238, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (320.36, 214.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 287679, 18, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327) [M::mem_pestat] mean and std.dev: (334.75, 250.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (249.90, 123.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1706, 5356, 5356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12656) [M::mem_pestat] mean and std.dev: (3530.33, 2007.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16306) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 268, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (347.28, 199.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702688 reads in 201.714 CPU sec, 6.694 real sec sorting block 93, #reads = 1081471 mem = 536883290 peak = 1091354624 [M::mem_process_seqs] Processed 702830 reads in 142.058 CPU sec, 4.615 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 115620, 10, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 307, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (276.41, 117.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (247.50, 123.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (680, 888, 1510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3170) [M::mem_pestat] mean and std.dev: (923.90, 408.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4000) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 192, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (215.05, 125.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 282796 reads in 51.165 CPU sec, 1.761 real sec sorting block 94, #reads = 1082623 mem = 536880625 peak = 1110122496 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 102599180 overall: 69999353856 mem 18761.350 user 423.541 sys 640.027 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_4ZB-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1136_4ZB-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_4ZB-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_4ZB-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_4ZB-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 640.065 sec; CPU: 19184.896 sec sorting block 95, #reads = 341134 mem = 169730719 peak = 1110122496 uniq header count 1 merging 96 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 5701292584 output reads: 103331478 bam_mem_sort: 96 calls 70.872 user 1.702 sys 74.174 real bam_write: 96 calls 75.117 user 5.050 sys 157.981 real execute: 1 calls 36.930 user 1.961 sys 685.452 real merge_files: 1 calls 36.896 user 1.940 sys 45.116 real parse_chunk: 1526 calls 95.115 user 23.818 sys 148.750 real read_chunk: 4128 calls 55.459 user 24.916 sys 589.865 real sort_block: 1 calls 39.133 user 24.251 sys 568.429 real write_chunk: 2600 calls 36.298 user 1.495 sys 43.595 real overall: 3066957824 mem 1777.300 user 78.565 sys 685.472 real ls: cannot access '*.cram': No such file or directory