This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_5TB-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1851.677 CPU sec, 70.309 real sec [M::process] read 701948 sequences (100000093 bp) in 1.308 CPU sec, 1.315 real sec... [M::process] read 701658 sequences (100000047 bp) in 1.833 CPU sec, 1.850 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286670, 12, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 245, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296) [M::mem_pestat] mean and std.dev: (402.50, 311.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 230, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (253.80, 124.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (297, 1149, 2931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8199) [M::mem_pestat] mean and std.dev: (1334.50, 1198.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 245, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (262.00, 127.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700198 sequences (100000065 bp) in 1.987 CPU sec, 2.034 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 286594, 23, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 332, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (513.64, 465.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 232, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (255.55, 125.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (189, 413, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1527) [M::mem_pestat] mean and std.dev: (403.14, 358.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 288, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862) [M::mem_pestat] mean and std.dev: (533.66, 527.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2644) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699480 sequences (100000089 bp) in 1.925 CPU sec, 1.969 real sec... [M::mem_process_seqs] Processed 701948 reads in 196.201 CPU sec, 6.271 real sec [M::mem_process_seqs] Processed 701658 reads in 131.414 CPU sec, 4.244 real sec sorting block 0, #reads = 1085046 mem = 536883893 peak = 598847488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286433, 22, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 220, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (269.57, 159.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 235, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (258.95, 127.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 653, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3754) [M::mem_pestat] mean and std.dev: (508.11, 498.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 307, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (330.15, 204.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699100 sequences (100000031 bp) in 2.802 CPU sec, 3.002 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286673, 28, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 544, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3911) [M::mem_pestat] mean and std.dev: (820.85, 916.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (262.21, 129.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 1070, 2898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7940) [M::mem_pestat] mean and std.dev: (1816.93, 1864.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 377, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (403.47, 312.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698626 sequences (100000098 bp) in 2.268 CPU sec, 2.274 real sec... [M::mem_process_seqs] Processed 700198 reads in 188.988 CPU sec, 6.038 real sec [M::mem_process_seqs] Processed 699480 reads in 129.782 CPU sec, 4.176 real sec sorting block 1, #reads = 1083865 mem = 536878308 peak = 724676608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 286121, 17, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 338, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (328.80, 162.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (263.14, 129.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (302, 494, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4979) [M::mem_pestat] mean and std.dev: (503.50, 427.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 273, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (246.04, 134.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698904 sequences (100000248 bp) in 2.810 CPU sec, 3.148 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 286119, 16, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 306, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (331.02, 185.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (265.58, 131.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (480, 798, 1598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3834) [M::mem_pestat] mean and std.dev: (862.40, 722.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4952) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 320, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (331.63, 195.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698450 sequences (100000138 bp) in 2.096 CPU sec, 2.112 real sec... [M::mem_process_seqs] Processed 699100 reads in 185.418 CPU sec, 5.936 real sec [M::mem_process_seqs] Processed 698626 reads in 126.879 CPU sec, 4.046 real sec sorting block 2, #reads = 1083657 mem = 536877488 peak = 860971008 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 286206, 29, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 236, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774) [M::mem_pestat] mean and std.dev: (460.89, 432.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (265.71, 131.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (685, 922, 8281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23473) [M::mem_pestat] mean and std.dev: (3190.66, 3480.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 31069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 366, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1490) [M::mem_pestat] mean and std.dev: (343.54, 221.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698198 sequences (100000190 bp) in 3.072 CPU sec, 3.392 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 285990, 16, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 304, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1451) [M::mem_pestat] mean and std.dev: (349.45, 289.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (267.00, 132.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 906, 2386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6934) [M::mem_pestat] mean and std.dev: (1454.94, 1989.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 277, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (291.54, 161.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698904 reads in 187.356 CPU sec, 6.018 real sec [M::process] read 698612 sequences (100000008 bp) in 2.509 CPU sec, 2.563 real sec... sorting block 3, #reads = 1084448 mem = 536877210 peak = 860971008 [M::mem_process_seqs] Processed 698450 reads in 130.592 CPU sec, 4.151 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 286172, 23, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 510, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861) [M::mem_pestat] mean and std.dev: (473.91, 352.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (266.87, 131.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (783, 1156, 2727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6615) [M::mem_pestat] mean and std.dev: (1559.04, 1249.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8559) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 516, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2401) [M::mem_pestat] mean and std.dev: (507.07, 414.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 4, #reads = 1083478 mem = 536878523 peak = 894054400 [M::process] read 697586 sequences (100000098 bp) in 2.924 CPU sec, 3.450 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 286765, 17, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 352, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1536) [M::mem_pestat] mean and std.dev: (374.45, 265.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (267.65, 133.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 559, 1204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3026) [M::mem_pestat] mean and std.dev: (663.19, 494.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 365, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832) [M::mem_pestat] mean and std.dev: (424.39, 292.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698198 reads in 185.397 CPU sec, 5.993 real sec [M::mem_process_seqs] Processed 698612 reads in 128.752 CPU sec, 4.110 real sec [M::process] read 697262 sequences (100000200 bp) in 2.910 CPU sec, 3.049 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285981, 11, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 326, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (322.44, 159.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (270.41, 134.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 716, 3268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9062) [M::mem_pestat] mean and std.dev: (1850.00, 2199.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 315, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (408.98, 304.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 5, #reads = 1084465 mem = 536878830 peak = 894054400 [M::process] read 697206 sequences (100000206 bp) in 3.076 CPU sec, 3.531 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285495, 22, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 385, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2069) [M::mem_pestat] mean and std.dev: (445.13, 369.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (270.93, 134.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (326, 1409, 1847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4889) [M::mem_pestat] mean and std.dev: (1174.59, 645.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 358, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483) [M::mem_pestat] mean and std.dev: (536.97, 453.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3238) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697586 reads in 184.924 CPU sec, 6.011 real sec [M::mem_process_seqs] Processed 697262 reads in 130.780 CPU sec, 4.220 real sec [M::process] read 696662 sequences (100000199 bp) in 3.160 CPU sec, 3.401 real sec... sorting block 6, #reads = 1085013 mem = 536878924 peak = 894054400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285754, 9, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 214, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (250.22, 121.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (271.41, 134.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 362, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (366.72, 250.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285707, 3, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 245, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (245.39, 82.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.08, 135.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 323, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1636) [M::mem_pestat] mean and std.dev: (426.35, 390.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696246 sequences (100000181 bp) in 3.072 CPU sec, 3.584 real sec... [M::mem_process_seqs] Processed 697206 reads in 184.379 CPU sec, 5.990 real sec [M::mem_process_seqs] Processed 696662 reads in 129.248 CPU sec, 4.170 real sec sorting block 7, #reads = 1084061 mem = 536878201 peak = 894054400 [M::process] read 696194 sequences (100000057 bp) in 3.099 CPU sec, 3.297 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285695, 16, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 192, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (207.43, 83.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.36, 134.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (365, 807, 1665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4265) [M::mem_pestat] mean and std.dev: (1067.67, 1051.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 265, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685) [M::mem_pestat] mean and std.dev: (717.56, 905.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4844) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285116, 14, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 302, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886) [M::mem_pestat] mean and std.dev: (420.88, 398.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.08, 136.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1194, 4221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11467) [M::mem_pestat] mean and std.dev: (2181.86, 1876.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15090) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 207, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (258.08, 140.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 8, #reads = 1084093 mem = 536879587 peak = 894054400 [M::process] read 696092 sequences (100000190 bp) in 2.965 CPU sec, 3.182 real sec... [M::mem_process_seqs] Processed 696246 reads in 186.710 CPU sec, 6.095 real sec [M::mem_process_seqs] Processed 696194 reads in 131.440 CPU sec, 4.257 real sec [M::process] read 695708 sequences (100000122 bp) in 3.037 CPU sec, 3.274 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 285436, 18, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 296, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156) [M::mem_pestat] mean and std.dev: (320.64, 170.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.27, 135.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (417, 959, 2418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6420) [M::mem_pestat] mean and std.dev: (1382.83, 1164.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 295, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (296.49, 144.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 9, #reads = 1084698 mem = 536877334 peak = 894054400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285610, 28, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 332, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (339.87, 221.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.32, 136.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (304, 635, 2228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6076) [M::mem_pestat] mean and std.dev: (1427.89, 1406.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8000) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 272, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288) [M::mem_pestat] mean and std.dev: (392.11, 361.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695946 sequences (100000221 bp) in 3.210 CPU sec, 3.527 real sec... [M::mem_process_seqs] Processed 696092 reads in 185.250 CPU sec, 6.034 real sec [M::mem_process_seqs] Processed 695708 reads in 129.489 CPU sec, 4.178 real sec sorting block 10, #reads = 1082821 mem = 536878684 peak = 894054400 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285245, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 317, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 654) [M::mem_pestat] mean and std.dev: (300.00, 103.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (275.93, 136.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (329, 329, 2463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6731) [M::mem_pestat] mean and std.dev: (1045.00, 1212.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 288, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2691) [M::mem_pestat] mean and std.dev: (523.10, 483.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695882 sequences (100000113 bp) in 3.309 CPU sec, 3.653 real sec... [M::mem_process_seqs] Processed 695946 reads in 106.521 CPU sec, 3.594 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 285556, 14, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 259, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (328.33, 215.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (275.76, 136.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 567, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1628) [M::mem_pestat] mean and std.dev: (590.64, 387.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 336, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2320) [M::mem_pestat] mean and std.dev: (558.33, 568.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695576 sequences (100000122 bp) in 3.430 CPU sec, 3.803 real sec... [M::mem_process_seqs] Processed 695882 reads in 105.772 CPU sec, 3.558 real sec sorting block 11, #reads = 1083068 mem = 536880800 peak = 894054400 [M::process] read 694994 sequences (100000153 bp) in 2.829 CPU sec, 3.196 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284886, 23, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 276, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1630) [M::mem_pestat] mean and std.dev: (404.78, 317.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (276.72, 136.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 592, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3788) [M::mem_pestat] mean and std.dev: (719.61, 526.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4925) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 326, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (337.97, 175.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284659, 12, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 307, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (279.30, 162.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (278.54, 138.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 208, 2107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6067) [M::mem_pestat] mean and std.dev: (685.36, 1155.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 251, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (234.06, 127.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694714 sequences (100000225 bp) in 2.814 CPU sec, 2.988 real sec... [M::mem_process_seqs] Processed 695576 reads in 185.873 CPU sec, 6.070 real sec sorting block 12, #reads = 1083111 mem = 536878012 peak = 894054400 [M::mem_process_seqs] Processed 694994 reads in 130.241 CPU sec, 4.212 real sec [M::process] read 694342 sequences (100000198 bp) in 2.727 CPU sec, 2.911 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284509, 15, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 214, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983) [M::mem_pestat] mean and std.dev: (391.07, 307.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (277.53, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 395, 1937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5429) [M::mem_pestat] mean and std.dev: (1239.87, 1646.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 260, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (271.95, 143.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 13, #reads = 1082981 mem = 536880381 peak = 918548480 [M::process] read 694084 sequences (100000165 bp) in 2.516 CPU sec, 2.724 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284529, 33, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 347, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (295.87, 157.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (279.31, 137.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (313, 1428, 5284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15226) [M::mem_pestat] mean and std.dev: (2974.06, 3525.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 265, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (275.88, 197.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694714 reads in 185.763 CPU sec, 5.989 real sec [M::mem_process_seqs] Processed 694342 reads in 129.905 CPU sec, 4.136 real sec [M::process] read 693728 sequences (100000170 bp) in 2.331 CPU sec, 2.418 real sec... sorting block 14, #reads = 1082777 mem = 536878298 peak = 918548480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284596, 14, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 215, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (271.91, 196.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (279.86, 136.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 811, 3205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8887) [M::mem_pestat] mean and std.dev: (1196.62, 1158.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 326, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1841) [M::mem_pestat] mean and std.dev: (395.35, 298.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693808 sequences (100000270 bp) in 2.737 CPU sec, 3.012 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 284543, 17, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 237, 866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2226) [M::mem_pestat] mean and std.dev: (408.49, 418.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (280.12, 136.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 461, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4448) [M::mem_pestat] mean and std.dev: (805.00, 916.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5914) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 357, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785) [M::mem_pestat] mean and std.dev: (487.47, 391.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694084 reads in 185.646 CPU sec, 5.976 real sec [M::process] read 693176 sequences (100000226 bp) in 2.242 CPU sec, 2.285 real sec... [M::mem_process_seqs] Processed 693728 reads in 130.341 CPU sec, 4.143 real sec sorting block 15, #reads = 1080550 mem = 536879653 peak = 918548480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 284193, 19, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 366, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331) [M::mem_pestat] mean and std.dev: (348.17, 243.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (278.92, 135.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 694, 1728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4880) [M::mem_pestat] mean and std.dev: (1273.05, 1553.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 307, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (286.33, 154.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692836 sequences (100000271 bp) in 2.767 CPU sec, 3.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 284152, 20, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (256.90, 130.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (279.34, 134.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 1213, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4733) [M::mem_pestat] mean and std.dev: (1012.63, 659.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 389, 1359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3653) [M::mem_pestat] mean and std.dev: (714.17, 750.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693808 reads in 185.503 CPU sec, 5.975 real sec [M::process] read 693112 sequences (100000131 bp) in 2.319 CPU sec, 2.362 real sec... sorting block 16, #reads = 1081769 mem = 536880250 peak = 918548480 [M::mem_process_seqs] Processed 693176 reads in 128.812 CPU sec, 4.107 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283941, 29, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 259, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (264.79, 140.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 259, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (280.90, 135.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (463, 784, 2026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5152) [M::mem_pestat] mean and std.dev: (1273.97, 1164.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6715) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 541, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4041) [M::mem_pestat] mean and std.dev: (990.41, 1204.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 17, #reads = 1081672 mem = 536879210 peak = 918548480 [M::process] read 693342 sequences (100000128 bp) in 2.800 CPU sec, 3.047 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 283694, 28, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 211, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (373.20, 359.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (280.64, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 589, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3099) [M::mem_pestat] mean and std.dev: (692.54, 602.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 299, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2095) [M::mem_pestat] mean and std.dev: (445.58, 382.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692836 reads in 188.135 CPU sec, 6.108 real sec [M::process] read 693238 sequences (100000260 bp) in 2.390 CPU sec, 2.440 real sec... [M::mem_process_seqs] Processed 693112 reads in 133.094 CPU sec, 4.287 real sec sorting block 18, #reads = 1079226 mem = 536881962 peak = 918548480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 284496, 24, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 249, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1759) [M::mem_pestat] mean and std.dev: (341.84, 374.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (280.63, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 831, 2166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6024) [M::mem_pestat] mean and std.dev: (1198.87, 1460.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 283, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (264.88, 110.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693310 sequences (100000008 bp) in 2.783 CPU sec, 3.037 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283656, 21, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 293, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2378) [M::mem_pestat] mean and std.dev: (454.64, 589.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 257, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (279.18, 135.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (131, 562, 3276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9566) [M::mem_pestat] mean and std.dev: (1452.05, 2375.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 337, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665) [M::mem_pestat] mean and std.dev: (441.19, 376.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693342 reads in 190.282 CPU sec, 6.168 real sec [M::process] read 693302 sequences (100000078 bp) in 2.310 CPU sec, 2.324 real sec... [M::mem_process_seqs] Processed 693238 reads in 133.843 CPU sec, 4.315 real sec sorting block 19, #reads = 1079226 mem = 536880669 peak = 918548480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284158, 21, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4341) [M::mem_pestat] mean and std.dev: (748.48, 835.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 257, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (277.15, 132.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 552, 3021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8925) [M::mem_pestat] mean and std.dev: (1307.62, 1644.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11877) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 336, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (355.68, 209.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692906 sequences (100000106 bp) in 2.853 CPU sec, 3.276 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284554, 23, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 230, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (233.20, 77.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 255, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (275.88, 132.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 921, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4065) [M::mem_pestat] mean and std.dev: (863.87, 653.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 299, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2991) [M::mem_pestat] mean and std.dev: (621.65, 541.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3908) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693310 reads in 191.623 CPU sec, 6.180 real sec [M::process] read 693358 sequences (100000088 bp) in 2.384 CPU sec, 2.412 real sec... [M::mem_process_seqs] Processed 693302 reads in 134.707 CPU sec, 4.283 real sec sorting block 20, #reads = 1078845 mem = 536880545 peak = 941379584 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284556, 24, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 296, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974) [M::mem_pestat] mean and std.dev: (317.07, 227.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 256, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (277.41, 133.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (749, 852, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027) [M::mem_pestat] mean and std.dev: (890.83, 452.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2702) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 281, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (379.83, 245.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1420) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692972 sequences (100000137 bp) in 2.768 CPU sec, 3.151 real sec... sorting block 21, #reads = 1079522 mem = 536879193 peak = 990146560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284068, 31, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 260, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (282.86, 144.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 256, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (276.73, 132.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (562, 787, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2698) [M::mem_pestat] mean and std.dev: (753.69, 319.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 294, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (323.55, 190.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692906 reads in 195.332 CPU sec, 6.395 real sec [M::process] read 693200 sequences (100000109 bp) in 2.217 CPU sec, 2.231 real sec... [M::mem_process_seqs] Processed 693358 reads in 137.626 CPU sec, 4.489 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 283961, 20, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 281, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (273.32, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 260, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (280.75, 135.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 945, 1666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4240) [M::mem_pestat] mean and std.dev: (1035.50, 841.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5527) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 243, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998) [M::mem_pestat] mean and std.dev: (274.47, 157.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 22, #reads = 1078518 mem = 536878289 peak = 990146560 [M::process] read 693028 sequences (100000233 bp) in 2.603 CPU sec, 3.053 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 284109, 26, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 291, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262) [M::mem_pestat] mean and std.dev: (339.65, 226.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (280.24, 135.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 292, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2708) [M::mem_pestat] mean and std.dev: (574.54, 543.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 264, 726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1778) [M::mem_pestat] mean and std.dev: (450.00, 376.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692972 reads in 194.185 CPU sec, 6.361 real sec [M::process] read 692936 sequences (100000109 bp) in 2.280 CPU sec, 2.306 real sec... [M::mem_process_seqs] Processed 693200 reads in 136.714 CPU sec, 4.509 real sec sorting block 23, #reads = 1076599 mem = 536881263 peak = 990146560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 283998, 17, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 282, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1864) [M::mem_pestat] mean and std.dev: (333.88, 275.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (280.93, 136.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (359, 611, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2042) [M::mem_pestat] mean and std.dev: (619.56, 486.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2603) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 241, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (292.81, 189.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692744 sequences (100000231 bp) in 2.762 CPU sec, 2.961 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284520, 12, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 221, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3857) [M::mem_pestat] mean and std.dev: (839.88, 1085.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (280.35, 136.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (249, 2227, 4031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11595) [M::mem_pestat] mean and std.dev: (1907.17, 1646.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 238, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (309.07, 184.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693028 reads in 192.894 CPU sec, 6.276 real sec [M::process] read 693636 sequences (100000265 bp) in 2.463 CPU sec, 2.558 real sec... [M::mem_process_seqs] Processed 692936 reads in 135.504 CPU sec, 4.391 real sec sorting block 24, #reads = 1077184 mem = 536887389 peak = 990146560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 283696, 20, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 234, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (316.73, 288.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 259, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (281.68, 137.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (384, 1348, 1935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5037) [M::mem_pestat] mean and std.dev: (1457.60, 1399.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7055) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 353, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (316.53, 175.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702348 sequences (100000179 bp) in 2.762 CPU sec, 3.272 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 284011, 18, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 269, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3103) [M::mem_pestat] mean and std.dev: (538.94, 577.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (280.40, 138.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 636, 1487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3935) [M::mem_pestat] mean and std.dev: (763.41, 590.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5159) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 318, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (368.12, 266.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692744 reads in 195.033 CPU sec, 6.326 real sec [M::process] read 700480 sequences (100000116 bp) in 2.400 CPU sec, 2.456 real sec... sorting block 25, #reads = 1077925 mem = 536877672 peak = 990146560 [M::mem_process_seqs] Processed 693636 reads in 139.843 CPU sec, 4.476 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 287100, 30, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 358, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (419.81, 340.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 230, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (253.80, 125.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (346, 1072, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5152) [M::mem_pestat] mean and std.dev: (1192.38, 1033.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 302, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (280.62, 93.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 26, #reads = 1076330 mem = 536880996 peak = 1004904448 [M::process] read 699906 sequences (100000157 bp) in 2.823 CPU sec, 3.037 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 286297, 20, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 605, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3031) [M::mem_pestat] mean and std.dev: (731.28, 595.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 234, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (258.62, 128.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (315, 636, 2449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6717) [M::mem_pestat] mean and std.dev: (795.53, 799.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 249, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (241.13, 118.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702348 reads in 188.979 CPU sec, 6.133 real sec [M::process] read 699200 sequences (100000217 bp) in 2.547 CPU sec, 2.565 real sec... [M::mem_process_seqs] Processed 700480 reads in 130.627 CPU sec, 4.188 real sec sorting block 27, #reads = 1084572 mem = 536879061 peak = 1004904448 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 286209, 20, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 359, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2088) [M::mem_pestat] mean and std.dev: (461.66, 422.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 236, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (260.66, 129.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (129, 501, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2868) [M::mem_pestat] mean and std.dev: (412.24, 385.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3781) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 303, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1508) [M::mem_pestat] mean and std.dev: (410.60, 344.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698730 sequences (100000114 bp) in 2.659 CPU sec, 2.963 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 286008, 20, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 268, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2121) [M::mem_pestat] mean and std.dev: (390.31, 314.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (264.78, 132.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (436, 741, 2561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6811) [M::mem_pestat] mean and std.dev: (1228.35, 1297.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 319, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3052) [M::mem_pestat] mean and std.dev: (577.72, 511.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699906 reads in 184.776 CPU sec, 5.964 real sec [M::process] read 698012 sequences (100000074 bp) in 2.222 CPU sec, 2.261 real sec... [M::mem_process_seqs] Processed 699200 reads in 128.685 CPU sec, 4.139 real sec sorting block 28, #reads = 1085771 mem = 536877972 peak = 1004904448 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286376, 8, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 234, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (370.15, 316.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (265.73, 131.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 310, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2476) [M::mem_pestat] mean and std.dev: (502.46, 607.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3253) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 698228 sequences (100000038 bp) in 2.817 CPU sec, 3.090 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 286159, 14, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 222, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (287.68, 210.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (266.65, 131.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1484, 1729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3875) [M::mem_pestat] mean and std.dev: (1424.21, 914.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 380, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3135) [M::mem_pestat] mean and std.dev: (555.49, 620.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698730 reads in 182.751 CPU sec, 5.887 real sec [M::process] read 698304 sequences (100000051 bp) in 2.283 CPU sec, 2.321 real sec... sorting block 29, #reads = 1085937 mem = 536884776 peak = 1004904448 [M::mem_process_seqs] Processed 698012 reads in 126.599 CPU sec, 4.070 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286125, 15, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 438, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (587.33, 501.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (266.88, 131.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (216, 330, 1613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4407) [M::mem_pestat] mean and std.dev: (726.60, 734.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 432, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3296) [M::mem_pestat] mean and std.dev: (571.92, 456.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 30, #reads = 1086675 mem = 536883094 peak = 1019580416 [M::process] read 698182 sequences (100000147 bp) in 2.699 CPU sec, 3.183 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 286215, 9, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 347, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1686) [M::mem_pestat] mean and std.dev: (418.38, 289.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (267.13, 131.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 309, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690) [M::mem_pestat] mean and std.dev: (346.14, 225.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698228 reads in 181.645 CPU sec, 5.971 real sec [M::process] read 697912 sequences (100000145 bp) in 2.314 CPU sec, 2.389 real sec... [M::mem_process_seqs] Processed 698304 reads in 127.562 CPU sec, 4.156 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 286159, 13, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 208, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (253.51, 162.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (268.08, 133.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (487, 656, 1152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2482) [M::mem_pestat] mean and std.dev: (758.00, 447.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3147) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 300, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1700) [M::mem_pestat] mean and std.dev: (394.27, 309.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2201) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 31, #reads = 1085432 mem = 536877214 peak = 1019580416 [M::process] read 697450 sequences (100000203 bp) in 3.072 CPU sec, 3.332 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286251, 17, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 397, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2895) [M::mem_pestat] mean and std.dev: (630.45, 625.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (268.60, 132.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 382, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1537) [M::mem_pestat] mean and std.dev: (363.41, 279.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 292, 1190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3276) [M::mem_pestat] mean and std.dev: (440.28, 400.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4319) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698182 reads in 185.186 CPU sec, 6.014 real sec [M::process] read 697102 sequences (100000075 bp) in 2.340 CPU sec, 2.405 real sec... [M::mem_process_seqs] Processed 697912 reads in 129.097 CPU sec, 4.178 real sec sorting block 32, #reads = 1085451 mem = 536879117 peak = 1019580416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285866, 12, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 288, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285) [M::mem_pestat] mean and std.dev: (312.47, 187.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (270.07, 133.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1112, 2596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6496) [M::mem_pestat] mean and std.dev: (1390.08, 1168.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 284, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1938) [M::mem_pestat] mean and std.dev: (344.81, 265.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2524) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697444 sequences (100000178 bp) in 2.725 CPU sec, 3.221 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 285837, 11, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 367, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (370.65, 208.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.48, 135.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1574, 1933, 2721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5015) [M::mem_pestat] mean and std.dev: (1493.22, 843.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6162) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 296, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (308.42, 156.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697450 reads in 180.664 CPU sec, 5.872 real sec [M::mem_process_seqs] Processed 697102 reads in 127.687 CPU sec, 4.110 real sec [M::process] read 696776 sequences (100000122 bp) in 2.358 CPU sec, 2.495 real sec... sorting block 33, #reads = 1085260 mem = 536881284 peak = 1019580416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286281, 26, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 207, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (208.11, 83.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (271.77, 135.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (289, 764, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4699) [M::mem_pestat] mean and std.dev: (940.72, 797.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 245, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (287.05, 143.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696522 sequences (100000144 bp) in 2.925 CPU sec, 3.336 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 285352, 18, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 275, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2058) [M::mem_pestat] mean and std.dev: (381.89, 334.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.21, 135.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (243, 467, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1560) [M::mem_pestat] mean and std.dev: (426.07, 199.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1999) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 241, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (296.97, 194.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697444 reads in 180.701 CPU sec, 5.853 real sec sorting block 34, #reads = 1084622 mem = 536878683 peak = 1019580416 [M::mem_process_seqs] Processed 696776 reads in 126.562 CPU sec, 4.094 real sec [M::process] read 695974 sequences (100000113 bp) in 2.429 CPU sec, 2.654 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285443, 22, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 289, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283) [M::mem_pestat] mean and std.dev: (372.16, 222.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.53, 134.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 506, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1375) [M::mem_pestat] mean and std.dev: (367.60, 212.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1783) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 346, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (355.38, 228.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1642) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 35, #reads = 1084990 mem = 536879755 peak = 1036849152 [M::process] read 695986 sequences (100000096 bp) in 2.841 CPU sec, 3.237 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284921, 12, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 256, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (239.44, 105.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (273.17, 133.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (88, 277, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3070) [M::mem_pestat] mean and std.dev: (509.73, 715.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4064) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 310, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (334.89, 180.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696522 reads in 183.208 CPU sec, 6.010 real sec [M::mem_process_seqs] Processed 695974 reads in 129.155 CPU sec, 4.207 real sec [M::process] read 695938 sequences (100000256 bp) in 2.574 CPU sec, 2.698 real sec... sorting block 36, #reads = 1084022 mem = 536878720 peak = 1036849152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 284916, 12, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 275, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853) [M::mem_pestat] mean and std.dev: (372.00, 339.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.64, 134.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (542, 576, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2171) [M::mem_pestat] mean and std.dev: (589.09, 320.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 316, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831) [M::mem_pestat] mean and std.dev: (457.64, 441.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695632 sequences (100000033 bp) in 2.707 CPU sec, 3.243 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285279, 23, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 211, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (215.91, 105.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (274.87, 135.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (149, 512, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3287) [M::mem_pestat] mean and std.dev: (595.71, 500.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4333) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 232, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (235.23, 88.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695986 reads in 182.800 CPU sec, 5.926 real sec [M::mem_process_seqs] Processed 695938 reads in 128.841 CPU sec, 4.126 real sec [M::process] read 695184 sequences (100000152 bp) in 2.706 CPU sec, 2.798 real sec... sorting block 37, #reads = 1083335 mem = 536879778 peak = 1036849152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 285004, 26, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 224, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2073) [M::mem_pestat] mean and std.dev: (428.69, 458.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (275.18, 135.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (332, 1362, 1616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4184) [M::mem_pestat] mean and std.dev: (1364.19, 1245.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 446, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1811) [M::mem_pestat] mean and std.dev: (563.13, 401.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695434 sequences (100000060 bp) in 2.946 CPU sec, 3.409 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 285222, 19, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 185, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (182.20, 69.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (276.71, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 319, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2197) [M::mem_pestat] mean and std.dev: (508.12, 517.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 297, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (339.97, 253.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695632 reads in 183.943 CPU sec, 5.972 real sec sorting block 38, #reads = 1082494 mem = 536880569 peak = 1036849152 [M::mem_process_seqs] Processed 695184 reads in 129.035 CPU sec, 4.115 real sec [M::process] read 695060 sequences (100000148 bp) in 2.477 CPU sec, 2.521 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285343, 16, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 367, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2178) [M::mem_pestat] mean and std.dev: (434.43, 399.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (276.09, 136.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1216, 1880, 2197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4159) [M::mem_pestat] mean and std.dev: (1899.75, 1218.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (250.06, 103.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 39, #reads = 1082935 mem = 536879783 peak = 1039011840 [M::process] read 695142 sequences (100000133 bp) in 2.748 CPU sec, 3.000 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284660, 16, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (200.28, 104.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (277.23, 136.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 451, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3158) [M::mem_pestat] mean and std.dev: (671.50, 658.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4151) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 364, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (343.61, 170.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695434 reads in 185.803 CPU sec, 6.088 real sec [M::process] read 695032 sequences (100000232 bp) in 2.165 CPU sec, 2.190 real sec... [M::mem_process_seqs] Processed 695060 reads in 129.688 CPU sec, 4.265 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285297, 18, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2226) [M::mem_pestat] mean and std.dev: (355.55, 366.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2921) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 40, #reads = 1082951 mem = 536880429 peak = 1039011840 [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (276.84, 136.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 507, 3246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9644) [M::mem_pestat] mean and std.dev: (1605.83, 2168.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12843) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 371, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (357.49, 199.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694844 sequences (100000259 bp) in 2.982 CPU sec, 3.409 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 284669, 21, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 372, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376) [M::mem_pestat] mean and std.dev: (422.38, 275.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (276.39, 136.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (870, 1459, 2388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5424) [M::mem_pestat] mean and std.dev: (1507.65, 837.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6942) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 304, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (258.24, 87.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695142 reads in 184.299 CPU sec, 6.007 real sec [M::mem_process_seqs] Processed 695032 reads in 129.705 CPU sec, 4.180 real sec [M::process] read 694822 sequences (100000150 bp) in 2.463 CPU sec, 2.544 real sec... sorting block 41, #reads = 1081931 mem = 536881315 peak = 1039011840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 285163, 26, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 262, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2479) [M::mem_pestat] mean and std.dev: (486.79, 478.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (277.61, 136.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (384, 872, 1961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5115) [M::mem_pestat] mean and std.dev: (1257.58, 1146.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 286, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (266.79, 105.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694754 sequences (100000206 bp) in 2.661 CPU sec, 2.864 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284476, 25, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 213, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (258.11, 175.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (277.51, 136.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (977, 3515, 6028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16130) [M::mem_pestat] mean and std.dev: (3520.88, 2584.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 326, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3169) [M::mem_pestat] mean and std.dev: (716.77, 700.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694844 reads in 186.057 CPU sec, 6.058 real sec [M::process] read 693778 sequences (100000298 bp) in 2.220 CPU sec, 2.231 real sec... [M::mem_process_seqs] Processed 694822 reads in 131.351 CPU sec, 4.292 real sec sorting block 42, #reads = 1081733 mem = 536879249 peak = 1039011840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284525, 34, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 289, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (365.18, 293.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (277.30, 135.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (674, 2053, 3340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8672) [M::mem_pestat] mean and std.dev: (2359.81, 2354.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 285, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723) [M::mem_pestat] mean and std.dev: (399.42, 307.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 43, #reads = 1080858 mem = 536878042 peak = 1039011840 [M::process] read 693806 sequences (100000067 bp) in 3.068 CPU sec, 3.398 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 284493, 18, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 320, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1314) [M::mem_pestat] mean and std.dev: (382.78, 273.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (278.36, 135.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 337, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2374) [M::mem_pestat] mean and std.dev: (301.07, 340.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 263, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (280.61, 143.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694754 reads in 187.069 CPU sec, 6.151 real sec [M::process] read 693468 sequences (100000007 bp) in 2.353 CPU sec, 2.447 real sec... [M::mem_process_seqs] Processed 693778 reads in 130.699 CPU sec, 4.332 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284000, 20, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 234, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (236.40, 119.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (277.82, 134.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (247, 718, 2142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5932) [M::mem_pestat] mean and std.dev: (1398.05, 1546.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 254, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (297.49, 143.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 44, #reads = 1081203 mem = 536878606 peak = 1039011840 [M::process] read 694152 sequences (100000101 bp) in 2.716 CPU sec, 3.049 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283843, 23, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 400, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4047) [M::mem_pestat] mean and std.dev: (716.80, 774.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (278.49, 135.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1178, 1526, 3006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6662) [M::mem_pestat] mean and std.dev: (1739.70, 1224.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8490) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 283, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (283.86, 116.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693806 reads in 188.597 CPU sec, 6.153 real sec [M::process] read 694028 sequences (100000121 bp) in 2.387 CPU sec, 2.505 real sec... [M::mem_process_seqs] Processed 693468 reads in 132.720 CPU sec, 4.320 real sec sorting block 45, #reads = 1080680 mem = 536879435 peak = 1039011840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284163, 20, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 257, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1786) [M::mem_pestat] mean and std.dev: (362.61, 294.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (277.02, 134.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 1133, 3534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10342) [M::mem_pestat] mean and std.dev: (1538.15, 1700.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 504, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166) [M::mem_pestat] mean and std.dev: (563.83, 413.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694378 sequences (100000189 bp) in 3.132 CPU sec, 3.476 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 284236, 15, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 229, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1642) [M::mem_pestat] mean and std.dev: (305.06, 267.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (276.47, 134.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (643, 861, 7767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22015) [M::mem_pestat] mean and std.dev: (2909.40, 3830.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29139) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 312, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3271) [M::mem_pestat] mean and std.dev: (666.62, 683.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694152 reads in 190.834 CPU sec, 6.210 real sec [M::process] read 694158 sequences (100000024 bp) in 2.370 CPU sec, 2.459 real sec... [M::mem_process_seqs] Processed 694028 reads in 132.588 CPU sec, 4.298 real sec sorting block 46, #reads = 1079830 mem = 536877510 peak = 1039011840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284610, 18, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 358, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (359.84, 211.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (276.13, 134.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 580, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2778) [M::mem_pestat] mean and std.dev: (537.27, 507.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 259, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (261.06, 114.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693742 sequences (100000297 bp) in 2.842 CPU sec, 3.292 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284092, 14, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 304, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (282.18, 114.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 253, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (275.33, 133.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 873, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3011) [M::mem_pestat] mean and std.dev: (730.08, 516.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 279, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (362.15, 244.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694378 reads in 185.396 CPU sec, 6.044 real sec sorting block 47, #reads = 1080012 mem = 536880651 peak = 1039011840 [M::mem_process_seqs] Processed 694158 reads in 130.947 CPU sec, 4.227 real sec [M::process] read 693446 sequences (100000120 bp) in 2.454 CPU sec, 2.639 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284459, 21, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 356, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2558) [M::mem_pestat] mean and std.dev: (568.33, 528.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 254, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (275.54, 132.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (580, 1199, 2720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7000) [M::mem_pestat] mean and std.dev: (1979.86, 2062.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 431, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2771) [M::mem_pestat] mean and std.dev: (633.30, 589.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3618) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 48, #reads = 1081296 mem = 536876952 peak = 1039011840 [M::process] read 693618 sequences (100000006 bp) in 2.816 CPU sec, 3.247 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284200, 16, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 242, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (317.39, 254.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (277.41, 134.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 395, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3550) [M::mem_pestat] mean and std.dev: (684.50, 722.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (279, 404, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1734) [M::mem_pestat] mean and std.dev: (475.40, 367.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693742 reads in 185.889 CPU sec, 6.141 real sec [M::mem_process_seqs] Processed 693446 reads in 131.343 CPU sec, 4.319 real sec [M::process] read 693586 sequences (100000156 bp) in 2.796 CPU sec, 3.016 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284681, 13, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 198, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (227.68, 159.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (277.28, 134.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1598, 3562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9442) [M::mem_pestat] mean and std.dev: (1958.77, 1635.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 282, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1695) [M::mem_pestat] mean and std.dev: (281.44, 210.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 49, #reads = 1080859 mem = 536878304 peak = 1039011840 [M::process] read 693404 sequences (100000154 bp) in 2.581 CPU sec, 3.096 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284294, 15, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 230, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (270.30, 133.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (277.61, 134.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 630, 1863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5101) [M::mem_pestat] mean and std.dev: (1055.33, 1056.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 325, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (359.93, 213.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693618 reads in 187.315 CPU sec, 6.178 real sec [M::mem_process_seqs] Processed 693586 reads in 130.948 CPU sec, 4.325 real sec [M::process] read 693150 sequences (100000210 bp) in 2.678 CPU sec, 2.864 real sec... sorting block 50, #reads = 1079790 mem = 536878657 peak = 1039011840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284321, 17, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 500, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (551.79, 532.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (279.53, 136.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 698, 2197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6413) [M::mem_pestat] mean and std.dev: (1012.24, 999.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8521) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 342, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1726) [M::mem_pestat] mean and std.dev: (427.26, 345.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693062 sequences (100000243 bp) in 2.766 CPU sec, 3.225 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284335, 16, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 283, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (256.53, 155.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (281.23, 137.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1160, 1840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4256) [M::mem_pestat] mean and std.dev: (1173.80, 860.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312) [M::mem_pestat] mean and std.dev: (276.62, 233.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693404 reads in 186.665 CPU sec, 6.078 real sec [M::mem_process_seqs] Processed 693150 reads in 132.436 CPU sec, 4.272 real sec sorting block 51, #reads = 1081065 mem = 536880363 peak = 1039011840 [M::process] read 692872 sequences (100000226 bp) in 2.579 CPU sec, 2.664 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284198, 20, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 259, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (316.75, 201.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (281.88, 138.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (303, 830, 1287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3255) [M::mem_pestat] mean and std.dev: (852.75, 686.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 234, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (245.46, 86.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 52, #reads = 1079233 mem = 536878213 peak = 1070731264 [M::process] read 700472 sequences (100000247 bp) in 2.817 CPU sec, 3.214 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283741, 17, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 209, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1731) [M::mem_pestat] mean and std.dev: (380.23, 347.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2252) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (281.77, 137.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (88, 629, 1866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5422) [M::mem_pestat] mean and std.dev: (672.67, 646.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7200) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 338, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (332.53, 148.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693062 reads in 190.788 CPU sec, 6.289 real sec [M::process] read 701266 sequences (100000160 bp) in 2.567 CPU sec, 2.677 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 287019, 12, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 442, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (461.96, 367.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (258.16, 127.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (724, 1846, 3709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9679) [M::mem_pestat] mean and std.dev: (2733.33, 2690.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 271, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (296.93, 157.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700960 sequences (100000247 bp) in 2.423 CPU sec, 2.467 real sec... [M::mem_process_seqs] Processed 700472 reads in 113.230 CPU sec, 3.679 real sec sorting block 53, #reads = 1080426 mem = 536878422 peak = 1070731264 [M::mem_process_seqs] Processed 692872 reads in 294.683 CPU sec, 9.591 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 287033, 23, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 266, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (339.33, 257.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 231, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (255.56, 126.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 810, 2776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7718) [M::mem_pestat] mean and std.dev: (1346.26, 1370.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 267, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (301.28, 168.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699102 sequences (100000034 bp) in 2.704 CPU sec, 3.143 real sec... sorting block 54, #reads = 1081010 mem = 536880757 peak = 1080008704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286436, 15, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 223, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (239.85, 165.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 233, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (257.71, 127.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (517, 751, 1648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3910) [M::mem_pestat] mean and std.dev: (969.57, 788.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 240, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (239.98, 126.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701266 reads in 215.615 CPU sec, 7.034 real sec [M::mem_process_seqs] Processed 700960 reads in 132.964 CPU sec, 4.397 real sec [M::process] read 698480 sequences (100000168 bp) in 2.430 CPU sec, 2.582 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286335, 26, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 343, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3117) [M::mem_pestat] mean and std.dev: (775.88, 886.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (263.75, 130.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (783, 1172, 3506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8952) [M::mem_pestat] mean and std.dev: (2233.19, 2127.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 468, 1677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4599) [M::mem_pestat] mean and std.dev: (958.95, 1118.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 55, #reads = 1086000 mem = 536878779 peak = 1080008704 [M::process] read 698042 sequences (100000087 bp) in 2.737 CPU sec, 3.010 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285536, 22, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (305.12, 197.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (265.16, 130.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 797, 1612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4506) [M::mem_pestat] mean and std.dev: (893.40, 865.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 316, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1647) [M::mem_pestat] mean and std.dev: (365.34, 256.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699102 reads in 187.322 CPU sec, 6.104 real sec [M::process] read 697706 sequences (100000172 bp) in 2.347 CPU sec, 2.470 real sec... [M::mem_process_seqs] Processed 698480 reads in 132.687 CPU sec, 4.378 real sec sorting block 56, #reads = 1085021 mem = 536880665 peak = 1080008704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285932, 15, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 224, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2877) [M::mem_pestat] mean and std.dev: (580.02, 716.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (267.94, 132.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 976, 2373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6683) [M::mem_pestat] mean and std.dev: (1509.27, 1598.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8838) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 332, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (300.89, 152.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697816 sequences (100000077 bp) in 2.878 CPU sec, 3.303 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285916, 8, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 275, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1446) [M::mem_pestat] mean and std.dev: (327.10, 305.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (268.26, 132.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 279, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1635) [M::mem_pestat] mean and std.dev: (385.32, 365.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698042 reads in 183.270 CPU sec, 6.006 real sec [M::mem_process_seqs] Processed 697706 reads in 128.931 CPU sec, 4.227 real sec [M::process] read 697114 sequences (100000072 bp) in 2.443 CPU sec, 2.545 real sec... sorting block 57, #reads = 1084179 mem = 536880163 peak = 1080008704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 285829, 26, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 279, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (328.97, 253.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (268.82, 133.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (294, 798, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3045) [M::mem_pestat] mean and std.dev: (894.79, 827.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 409, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227) [M::mem_pestat] mean and std.dev: (364.81, 209.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697018 sequences (100000259 bp) in 2.732 CPU sec, 3.295 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 285197, 22, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 263, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152) [M::mem_pestat] mean and std.dev: (479.60, 389.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (270.17, 133.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (482, 994, 3512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9572) [M::mem_pestat] mean and std.dev: (1738.73, 1880.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 345, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (422.95, 308.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697816 reads in 187.218 CPU sec, 6.116 real sec sorting block 58, #reads = 1084069 mem = 536880724 peak = 1080008704 [M::mem_process_seqs] Processed 697114 reads in 131.710 CPU sec, 4.267 real sec [M::process] read 696636 sequences (100000102 bp) in 2.291 CPU sec, 2.523 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 285912, 26, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 315, 1869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5231) [M::mem_pestat] mean and std.dev: (1029.90, 1317.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.41, 135.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (443, 762, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3755) [M::mem_pestat] mean and std.dev: (699.23, 548.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 297, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (336.53, 207.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 59, #reads = 1084020 mem = 536879906 peak = 1080008704 [M::process] read 696712 sequences (100000089 bp) in 2.738 CPU sec, 3.290 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285632, 26, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 316, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (384.42, 292.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (273.17, 135.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (428, 672, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3122) [M::mem_pestat] mean and std.dev: (857.35, 652.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 268, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (313.43, 209.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697018 reads in 182.549 CPU sec, 5.974 real sec [M::mem_process_seqs] Processed 696636 reads in 129.082 CPU sec, 4.200 real sec [M::process] read 696752 sequences (100000178 bp) in 2.539 CPU sec, 2.725 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285619, 26, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 423, 1347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3619) [M::mem_pestat] mean and std.dev: (643.48, 666.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (273.74, 136.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (113, 551, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427) [M::mem_pestat] mean and std.dev: (398.76, 239.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 447, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1960) [M::mem_pestat] mean and std.dev: (514.45, 353.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 60, #reads = 1083989 mem = 536877773 peak = 1080008704 [M::process] read 696276 sequences (100000203 bp) in 2.871 CPU sec, 3.291 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285378, 18, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205) [M::mem_pestat] mean and std.dev: (390.45, 249.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.89, 136.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 842, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2712) [M::mem_pestat] mean and std.dev: (966.06, 756.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3992) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 298, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (319.82, 220.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696712 reads in 181.246 CPU sec, 5.905 real sec [M::mem_process_seqs] Processed 696752 reads in 127.988 CPU sec, 4.098 real sec [M::process] read 695992 sequences (100000250 bp) in 2.731 CPU sec, 2.885 real sec... sorting block 61, #reads = 1084047 mem = 536880206 peak = 1080008704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285607, 13, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 242, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (391.81, 360.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (275.48, 137.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (323, 1181, 5154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14816) [M::mem_pestat] mean and std.dev: (2876.92, 3437.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19647) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 354, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (334.97, 190.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695830 sequences (100000296 bp) in 2.681 CPU sec, 3.379 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285787, 7, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 303, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (318.27, 163.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (277.32, 139.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 255, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (254.97, 114.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696276 reads in 181.570 CPU sec, 5.954 real sec [M::mem_process_seqs] Processed 695992 reads in 127.293 CPU sec, 4.137 real sec sorting block 62, #reads = 1083556 mem = 536881066 peak = 1080008704 [M::process] read 695504 sequences (100000077 bp) in 2.466 CPU sec, 2.637 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285471, 24, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 289, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (302.00, 169.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.34, 137.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 1075, 4016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11608) [M::mem_pestat] mean and std.dev: (2447.88, 2916.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 354, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2858) [M::mem_pestat] mean and std.dev: (493.48, 464.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3753) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 63, #reads = 1083441 mem = 536879695 peak = 1080008704 [M::process] read 694822 sequences (100000269 bp) in 2.737 CPU sec, 3.169 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284801, 17, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 246, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (271.76, 140.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (277.52, 138.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 1112, 1802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5198) [M::mem_pestat] mean and std.dev: (988.38, 1018.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 277, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (287.40, 131.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695830 reads in 182.673 CPU sec, 5.997 real sec [M::mem_process_seqs] Processed 695504 reads in 128.655 CPU sec, 4.182 real sec [M::process] read 695576 sequences (100000076 bp) in 2.407 CPU sec, 2.523 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285354, 24, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 274, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (317.08, 194.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (278.76, 138.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 455, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4897) [M::mem_pestat] mean and std.dev: (1049.77, 958.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 381, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2441) [M::mem_pestat] mean and std.dev: (548.38, 447.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3175) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 64, #reads = 1083582 mem = 536879618 peak = 1080008704 [M::process] read 696884 sequences (100000212 bp) in 2.636 CPU sec, 3.233 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 285214, 18, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 593, 1487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3793) [M::mem_pestat] mean and std.dev: (921.45, 1022.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.04, 138.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (280, 724, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4054) [M::mem_pestat] mean and std.dev: (904.39, 667.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 354, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2626) [M::mem_pestat] mean and std.dev: (614.46, 660.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694822 reads in 180.710 CPU sec, 5.934 real sec [M::mem_process_seqs] Processed 695576 reads in 127.172 CPU sec, 4.135 real sec [M::process] read 695148 sequences (100000236 bp) in 2.483 CPU sec, 2.537 real sec... sorting block 65, #reads = 1082403 mem = 536883860 peak = 1080008704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285367, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 286, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2529) [M::mem_pestat] mean and std.dev: (447.56, 479.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (271.21, 134.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (141, 2334, 6005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17733) [M::mem_pestat] mean and std.dev: (2749.67, 2657.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (269.74, 129.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694720 sequences (100000285 bp) in 2.817 CPU sec, 3.361 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284607, 18, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 220, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (241.17, 130.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (277.16, 137.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (403, 879, 2996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8182) [M::mem_pestat] mean and std.dev: (1374.33, 1193.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 319, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472) [M::mem_pestat] mean and std.dev: (623.90, 632.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696884 reads in 183.210 CPU sec, 5.953 real sec [M::mem_process_seqs] Processed 695148 reads in 128.628 CPU sec, 4.192 real sec [M::process] read 694334 sequences (100000185 bp) in 2.350 CPU sec, 2.523 real sec... sorting block 66, #reads = 1083456 mem = 536881884 peak = 1080008704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 284897, 22, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 363, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1948) [M::mem_pestat] mean and std.dev: (478.25, 400.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (279.48, 138.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (216, 808, 1386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3726) [M::mem_pestat] mean and std.dev: (910.10, 1028.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5024) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 381, 1040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2670) [M::mem_pestat] mean and std.dev: (510.87, 492.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694142 sequences (100000062 bp) in 2.575 CPU sec, 3.083 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284933, 39, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 318, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204) [M::mem_pestat] mean and std.dev: (647.86, 625.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (280.07, 138.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 843, 2710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7486) [M::mem_pestat] mean and std.dev: (1363.66, 1566.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9874) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 369, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (409.66, 253.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1424) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694720 reads in 183.581 CPU sec, 5.951 real sec sorting block 67, #reads = 1082553 mem = 536878873 peak = 1095512064 [M::mem_process_seqs] Processed 694334 reads in 129.075 CPU sec, 4.147 real sec [M::process] read 694018 sequences (100000043 bp) in 2.395 CPU sec, 2.529 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 284625, 19, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 234, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567) [M::mem_pestat] mean and std.dev: (299.16, 247.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (278.08, 135.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 422, 1311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3453) [M::mem_pestat] mean and std.dev: (777.11, 738.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 323, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (322.92, 145.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 68, #reads = 1082318 mem = 536879124 peak = 1098866688 [M::process] read 693448 sequences (100000190 bp) in 2.953 CPU sec, 3.340 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284050, 20, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 230, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (208.57, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (279.70, 137.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 818, 3707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10791) [M::mem_pestat] mean and std.dev: (1525.80, 1625.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14333) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 280, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527) [M::mem_pestat] mean and std.dev: (482.12, 503.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694142 reads in 183.574 CPU sec, 5.928 real sec [M::mem_process_seqs] Processed 694018 reads in 129.910 CPU sec, 4.174 real sec [M::process] read 693476 sequences (100000150 bp) in 2.431 CPU sec, 2.558 real sec... sorting block 69, #reads = 1081260 mem = 536876760 peak = 1098866688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 284564, 21, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 259, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412) [M::mem_pestat] mean and std.dev: (319.88, 228.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (280.71, 137.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 1008, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4550) [M::mem_pestat] mean and std.dev: (858.85, 769.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6024) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 445, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1993) [M::mem_pestat] mean and std.dev: (565.87, 451.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2574) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693506 sequences (100000292 bp) in 2.697 CPU sec, 3.183 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284382, 16, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 245, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1241) [M::mem_pestat] mean and std.dev: (329.35, 252.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (280.45, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (712, 1273, 2922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7342) [M::mem_pestat] mean and std.dev: (1807.00, 1541.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 306, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (378.49, 228.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693448 reads in 181.604 CPU sec, 5.907 real sec [M::mem_process_seqs] Processed 693476 reads in 128.020 CPU sec, 4.147 real sec [M::process] read 694060 sequences (100000174 bp) in 2.425 CPU sec, 2.475 real sec... sorting block 70, #reads = 1081398 mem = 536878890 peak = 1098866688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284584, 14, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 248, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (294.57, 198.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (278.90, 136.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (301, 1011, 1798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4792) [M::mem_pestat] mean and std.dev: (1146.92, 1337.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 367, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108) [M::mem_pestat] mean and std.dev: (535.19, 468.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693804 sequences (100000198 bp) in 2.776 CPU sec, 3.361 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284156, 9, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 366, 883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2213) [M::mem_pestat] mean and std.dev: (498.74, 429.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (277.22, 136.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 415, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (426.91, 273.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693506 reads in 185.805 CPU sec, 6.038 real sec [M::process] read 693878 sequences (100000159 bp) in 2.296 CPU sec, 2.352 real sec... [M::mem_process_seqs] Processed 694060 reads in 131.243 CPU sec, 4.192 real sec sorting block 71, #reads = 1082240 mem = 536879010 peak = 1098866688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 284373, 13, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 297, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998) [M::mem_pestat] mean and std.dev: (282.27, 151.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (277.56, 135.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (471, 617, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1929) [M::mem_pestat] mean and std.dev: (504.64, 282.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 231, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (276.63, 164.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 72, #reads = 1080731 mem = 536879201 peak = 1105801216 [M::process] read 694110 sequences (100000088 bp) in 2.705 CPU sec, 3.199 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284647, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 394, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2976) [M::mem_pestat] mean and std.dev: (652.74, 722.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 253, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (276.45, 135.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 294, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312) [M::mem_pestat] mean and std.dev: (311.54, 181.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693804 reads in 184.308 CPU sec, 6.030 real sec [M::mem_process_seqs] Processed 693878 reads in 132.121 CPU sec, 4.290 real sec [M::process] read 694782 sequences (100000261 bp) in 2.416 CPU sec, 2.606 real sec... sorting block 73, #reads = 1080823 mem = 536882371 peak = 1105801216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 284867, 12, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 267, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288) [M::mem_pestat] mean and std.dev: (334.70, 263.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 253, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (276.42, 135.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (215, 1127, 3182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9116) [M::mem_pestat] mean and std.dev: (1660.42, 1941.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 252, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1323) [M::mem_pestat] mean and std.dev: (407.27, 307.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694156 sequences (100000037 bp) in 2.692 CPU sec, 3.316 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 285005, 20, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (253.32, 142.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (275.08, 135.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (141, 546, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1740) [M::mem_pestat] mean and std.dev: (456.89, 368.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2273) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 353, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (334.76, 169.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694110 reads in 188.573 CPU sec, 6.187 real sec [M::process] read 693760 sequences (100000287 bp) in 2.359 CPU sec, 2.522 real sec... [M::mem_process_seqs] Processed 694782 reads in 132.884 CPU sec, 4.346 real sec sorting block 74, #reads = 1080206 mem = 536882745 peak = 1105801216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284472, 13, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 242, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2717) [M::mem_pestat] mean and std.dev: (354.65, 384.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (277.11, 136.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (278, 1107, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7262) [M::mem_pestat] mean and std.dev: (1310.83, 1060.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 333, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611) [M::mem_pestat] mean and std.dev: (415.38, 333.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693638 sequences (100000258 bp) in 2.949 CPU sec, 3.441 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 284397, 23, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 260, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (264.54, 140.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (277.64, 135.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 319, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2667) [M::mem_pestat] mean and std.dev: (469.10, 348.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3462) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 292, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1489) [M::mem_pestat] mean and std.dev: (402.25, 315.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694156 reads in 183.882 CPU sec, 5.952 real sec [M::mem_process_seqs] Processed 693760 reads in 131.201 CPU sec, 4.208 real sec sorting block 75, #reads = 1080833 mem = 536878531 peak = 1105801216 [M::process] read 693338 sequences (100000219 bp) in 2.626 CPU sec, 2.724 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284374, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 221, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (256.32, 143.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (279.27, 135.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 237, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (299.64, 214.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693180 sequences (100000001 bp) in 2.806 CPU sec, 3.141 real sec... sorting block 76, #reads = 1079235 mem = 536879653 peak = 1105801216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284033, 26, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 275, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (252.52, 111.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (279.17, 135.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (278, 491, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (370.77, 202.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 292, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1414) [M::mem_pestat] mean and std.dev: (406.50, 277.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693638 reads in 188.351 CPU sec, 6.089 real sec [M::mem_process_seqs] Processed 693338 reads in 134.165 CPU sec, 4.312 real sec [M::process] read 693378 sequences (100000071 bp) in 2.409 CPU sec, 2.583 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283948, 12, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 356, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653) [M::mem_pestat] mean and std.dev: (419.20, 314.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (279.88, 136.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (440, 667, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3524) [M::mem_pestat] mean and std.dev: (592.90, 383.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 253, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (289.85, 114.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 77, #reads = 1079244 mem = 536878127 peak = 1105801216 [M::process] read 693172 sequences (100000106 bp) in 2.706 CPU sec, 3.269 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 283629, 38, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 285, 1171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3185) [M::mem_pestat] mean and std.dev: (644.52, 777.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (281.32, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 676, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3332) [M::mem_pestat] mean and std.dev: (617.45, 518.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 280, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (298.11, 161.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693180 reads in 186.507 CPU sec, 6.080 real sec [M::process] read 693334 sequences (100000033 bp) in 2.247 CPU sec, 2.343 real sec... [M::mem_process_seqs] Processed 693378 reads in 131.051 CPU sec, 4.272 real sec sorting block 78, #reads = 1078151 mem = 536880965 peak = 1105801216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 284475, 16, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 283, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (266.23, 116.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (281.97, 138.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 1617, 2525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7193) [M::mem_pestat] mean and std.dev: (1319.06, 1007.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9527) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 256, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (341.42, 228.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697052 sequences (100000094 bp) in 2.806 CPU sec, 3.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 284297, 12, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 460, 1205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3171) [M::mem_pestat] mean and std.dev: (586.08, 581.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 259, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (281.90, 138.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (437, 1085, 1950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4976) [M::mem_pestat] mean and std.dev: (1075.25, 717.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6489) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 300, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2050) [M::mem_pestat] mean and std.dev: (442.88, 421.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693172 reads in 190.322 CPU sec, 6.191 real sec [M::process] read 701328 sequences (100000137 bp) in 2.353 CPU sec, 2.452 real sec... [M::mem_process_seqs] Processed 693334 reads in 135.852 CPU sec, 4.373 real sec sorting block 79, #reads = 1078750 mem = 536880444 peak = 1105801216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 284669, 19, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 193, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (228.51, 156.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.31, 135.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (773, 1247, 1616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302) [M::mem_pestat] mean and std.dev: (1013.53, 545.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4145) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 358, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1783) [M::mem_pestat] mean and std.dev: (441.21, 445.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701380 sequences (100000068 bp) in 3.005 CPU sec, 3.552 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 287030, 16, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 249, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (349.58, 287.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (255.08, 126.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 324, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (304.31, 126.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 290, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1624) [M::mem_pestat] mean and std.dev: (380.47, 262.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697052 reads in 196.317 CPU sec, 6.344 real sec [M::process] read 700460 sequences (100000108 bp) in 2.320 CPU sec, 2.376 real sec... sorting block 80, #reads = 1077102 mem = 536882587 peak = 1105801216 [M::mem_process_seqs] Processed 701328 reads in 132.996 CPU sec, 4.266 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 287179, 21, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 230, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2107) [M::mem_pestat] mean and std.dev: (433.84, 465.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 231, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (254.71, 125.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 274, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4340) [M::mem_pestat] mean and std.dev: (928.76, 1310.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6170) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 311, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (296.40, 128.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 81, #reads = 1081413 mem = 536881914 peak = 1107607552 [M::process] read 698104 sequences (100000028 bp) in 3.044 CPU sec, 3.337 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 287109, 30, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 282, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (330.52, 178.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (260.60, 130.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (548, 773, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4400) [M::mem_pestat] mean and std.dev: (1026.21, 699.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 281, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (277.90, 120.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701380 reads in 182.604 CPU sec, 5.954 real sec [M::mem_process_seqs] Processed 700460 reads in 127.185 CPU sec, 4.082 real sec [M::process] read 697642 sequences (100000064 bp) in 2.420 CPU sec, 2.484 real sec... sorting block 82, #reads = 1086561 mem = 536880490 peak = 1107607552 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286004, 23, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 219, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (252.08, 134.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (267.43, 133.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 617, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3365) [M::mem_pestat] mean and std.dev: (642.57, 616.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 284, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983) [M::mem_pestat] mean and std.dev: (506.72, 512.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697716 sequences (100000069 bp) in 2.632 CPU sec, 3.352 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285872, 27, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 306, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (310.43, 182.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (269.83, 134.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 487, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3345) [M::mem_pestat] mean and std.dev: (662.56, 670.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 274, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (282.74, 146.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698104 reads in 179.431 CPU sec, 5.892 real sec [M::mem_process_seqs] Processed 697642 reads in 127.027 CPU sec, 4.139 real sec [M::process] read 697400 sequences (100000118 bp) in 2.589 CPU sec, 2.832 real sec... sorting block 83, #reads = 1086092 mem = 536884176 peak = 1107607552 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 286126, 25, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 349, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (291.94, 136.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (269.79, 135.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (209, 467, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3626) [M::mem_pestat] mean and std.dev: (732.36, 685.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 298, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547) [M::mem_pestat] mean and std.dev: (427.37, 311.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696846 sequences (100000101 bp) in 2.989 CPU sec, 3.292 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285291, 15, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339) [M::mem_pestat] mean and std.dev: (316.29, 314.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.91, 136.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (469, 541, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381) [M::mem_pestat] mean and std.dev: (497.69, 217.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 372, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (387.03, 214.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697716 reads in 181.467 CPU sec, 5.849 real sec [M::mem_process_seqs] Processed 697400 reads in 127.356 CPU sec, 4.095 real sec sorting block 84, #reads = 1085227 mem = 536881024 peak = 1107607552 [M::process] read 696952 sequences (100000078 bp) in 2.353 CPU sec, 2.418 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285501, 14, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 322, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (303.29, 149.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (272.88, 136.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (548, 1289, 2800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7304) [M::mem_pestat] mean and std.dev: (1402.23, 1370.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9556) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 248, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (369.62, 275.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 85, #reads = 1085058 mem = 536879434 peak = 1107607552 [M::process] read 696806 sequences (100000196 bp) in 2.648 CPU sec, 3.347 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285889, 17, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 315, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (273.59, 153.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (272.47, 135.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (131, 233, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4214) [M::mem_pestat] mean and std.dev: (402.07, 631.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5575) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 279, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (295.68, 167.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696846 reads in 179.555 CPU sec, 5.877 real sec [M::mem_process_seqs] Processed 696952 reads in 127.435 CPU sec, 4.084 real sec [M::process] read 696044 sequences (100000263 bp) in 2.860 CPU sec, 2.958 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 285627, 12, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 307, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285) [M::mem_pestat] mean and std.dev: (414.92, 317.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (274.11, 137.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1465, 2402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6006) [M::mem_pestat] mean and std.dev: (1247.55, 1037.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7808) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 349, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340) [M::mem_pestat] mean and std.dev: (371.63, 282.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 86, #reads = 1084097 mem = 536878803 peak = 1107607552 [M::process] read 695592 sequences (100000162 bp) in 2.688 CPU sec, 3.311 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285162, 22, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 294, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (299.79, 160.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (275.91, 137.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (203, 505, 1716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4742) [M::mem_pestat] mean and std.dev: (1140.55, 1332.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 276, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1749) [M::mem_pestat] mean and std.dev: (377.65, 312.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696806 reads in 179.801 CPU sec, 5.887 real sec [M::mem_process_seqs] Processed 696044 reads in 126.271 CPU sec, 4.093 real sec [M::process] read 695782 sequences (100000089 bp) in 2.706 CPU sec, 2.840 real sec... sorting block 87, #reads = 1083712 mem = 536878131 peak = 1107607552 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285455, 18, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 282, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1992) [M::mem_pestat] mean and std.dev: (379.81, 309.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (277.58, 138.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (549, 958, 3992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10878) [M::mem_pestat] mean and std.dev: (2616.50, 3270.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 308, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (322.63, 153.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695734 sequences (100000095 bp) in 2.562 CPU sec, 2.985 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285044, 9, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 264, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346) [M::mem_pestat] mean and std.dev: (344.68, 310.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (276.37, 138.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 250, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (230.95, 71.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695592 reads in 182.986 CPU sec, 5.931 real sec [M::mem_process_seqs] Processed 695782 reads in 128.929 CPU sec, 4.122 real sec sorting block 88, #reads = 1083456 mem = 536879379 peak = 1107607552 [M::process] read 695568 sequences (100000093 bp) in 2.575 CPU sec, 2.767 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 284733, 13, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 286, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (300.33, 170.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.06, 138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (694, 1026, 1679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3649) [M::mem_pestat] mean and std.dev: (1171.38, 773.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (324, 518, 1530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3942) [M::mem_pestat] mean and std.dev: (739.70, 693.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694902 sequences (100000177 bp) in 2.878 CPU sec, 3.237 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285547, 16, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 354, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2277) [M::mem_pestat] mean and std.dev: (543.33, 402.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (278.44, 139.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 1454, 4139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11943) [M::mem_pestat] mean and std.dev: (2101.25, 2178.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 420, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1903) [M::mem_pestat] mean and std.dev: (537.65, 414.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695734 reads in 180.438 CPU sec, 5.865 real sec sorting block 89, #reads = 1084810 mem = 536877528 peak = 1107607552 [M::mem_process_seqs] Processed 695568 reads in 128.417 CPU sec, 4.110 real sec [M::process] read 695060 sequences (100000044 bp) in 2.330 CPU sec, 2.519 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285088, 24, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2420) [M::mem_pestat] mean and std.dev: (357.50, 295.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (278.61, 138.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (573, 1138, 1858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4428) [M::mem_pestat] mean and std.dev: (1225.46, 971.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 311, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (288.61, 126.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 90, #reads = 1083257 mem = 536878290 peak = 1125826560 [M::process] read 695062 sequences (100000227 bp) in 2.686 CPU sec, 3.105 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285399, 25, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 369, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437) [M::mem_pestat] mean and std.dev: (422.09, 324.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (279.42, 139.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (499, 784, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828) [M::mem_pestat] mean and std.dev: (682.33, 337.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 286, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (285.82, 152.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694902 reads in 180.982 CPU sec, 5.979 real sec [M::process] read 695902 sequences (100000233 bp) in 2.289 CPU sec, 2.420 real sec... [M::mem_process_seqs] Processed 695060 reads in 128.780 CPU sec, 4.303 real sec sorting block 91, #reads = 1082229 mem = 536882554 peak = 1125826560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285498, 11, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 373, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2436) [M::mem_pestat] mean and std.dev: (392.31, 302.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (279.68, 139.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 224, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (172, 302) [M::mem_pestat] mean and std.dev: (234.43, 17.42) [M::mem_pestat] low and high boundaries for proper pairs: (146, 328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 311, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (360.76, 214.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695862 sequences (100000201 bp) in 2.877 CPU sec, 3.623 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 285120, 22, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 423, 2089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5747) [M::mem_pestat] mean and std.dev: (1168.91, 1560.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.59, 136.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (106, 1216, 3364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9880) [M::mem_pestat] mean and std.dev: (1638.59, 1790.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 448, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2067) [M::mem_pestat] mean and std.dev: (552.94, 512.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695062 reads in 181.045 CPU sec, 5.900 real sec [M::mem_process_seqs] Processed 695902 reads in 128.296 CPU sec, 4.157 real sec [M::process] read 694366 sequences (100000097 bp) in 2.439 CPU sec, 2.627 real sec... sorting block 92, #reads = 1083002 mem = 536879305 peak = 1125826560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284829, 18, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 248, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (339.24, 286.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.64, 136.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (438, 1061, 2661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7107) [M::mem_pestat] mean and std.dev: (1437.33, 1128.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 292, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (372.72, 235.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1568) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693772 sequences (100000210 bp) in 2.459 CPU sec, 3.172 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285169, 15, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 291, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (304.67, 165.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (280.21, 138.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (542, 1040, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4556) [M::mem_pestat] mean and std.dev: (1019.53, 655.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 333, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (265.53, 157.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695862 reads in 181.337 CPU sec, 5.895 real sec sorting block 93, #reads = 1083390 mem = 536878539 peak = 1129381888 [M::mem_process_seqs] Processed 694366 reads in 126.348 CPU sec, 4.059 real sec [M::process] read 693952 sequences (100000149 bp) in 2.549 CPU sec, 2.615 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 284326, 20, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 379, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2427) [M::mem_pestat] mean and std.dev: (475.50, 400.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (281.77, 139.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (526, 1148, 2548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6592) [M::mem_pestat] mean and std.dev: (1324.45, 918.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8614) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 271, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3289) [M::mem_pestat] mean and std.dev: (510.38, 521.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 94, #reads = 1082638 mem = 536877595 peak = 1129381888 [M::process] read 693556 sequences (100000225 bp) in 2.843 CPU sec, 3.434 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284648, 14, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 237, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (232.04, 88.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (281.26, 138.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (380, 421, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3164) [M::mem_pestat] mean and std.dev: (933.29, 787.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 304, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (279.75, 121.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693772 reads in 181.024 CPU sec, 5.986 real sec [M::mem_process_seqs] Processed 693952 reads in 127.595 CPU sec, 4.297 real sec [M::process] read 693232 sequences (100000025 bp) in 2.394 CPU sec, 2.512 real sec... sorting block 95, #reads = 1082217 mem = 536876899 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 283897, 14, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 315, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (315.36, 176.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (279.10, 135.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 738, 2336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6866) [M::mem_pestat] mean and std.dev: (1390.21, 1610.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 245, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (278.09, 166.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692912 sequences (100000046 bp) in 2.727 CPU sec, 2.954 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 283850, 26, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 323, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1616) [M::mem_pestat] mean and std.dev: (350.52, 267.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 257, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (279.27, 135.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 839, 2914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8108) [M::mem_pestat] mean and std.dev: (1797.00, 1871.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2670) [M::mem_pestat] mean and std.dev: (532.94, 582.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693556 reads in 185.701 CPU sec, 6.072 real sec [M::process] read 693236 sequences (100000232 bp) in 2.327 CPU sec, 2.477 real sec... [M::mem_process_seqs] Processed 693232 reads in 130.440 CPU sec, 4.256 real sec sorting block 96, #reads = 1081210 mem = 536880582 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 283890, 22, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 282, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (290.57, 148.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (279.28, 135.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 492, 1558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3964) [M::mem_pestat] mean and std.dev: (767.58, 810.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 315, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1704) [M::mem_pestat] mean and std.dev: (376.13, 288.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693556 sequences (100000071 bp) in 2.793 CPU sec, 3.075 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 283942, 21, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 342, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3499) [M::mem_pestat] mean and std.dev: (773.06, 894.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (277.84, 134.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (815, 988, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2978) [M::mem_pestat] mean and std.dev: (933.56, 415.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3699) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 345, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432) [M::mem_pestat] mean and std.dev: (440.48, 349.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692912 reads in 185.346 CPU sec, 6.006 real sec [M::process] read 693256 sequences (100000286 bp) in 2.322 CPU sec, 2.368 real sec... [M::mem_process_seqs] Processed 693236 reads in 131.337 CPU sec, 4.259 real sec sorting block 97, #reads = 1080345 mem = 536878642 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 284494, 13, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 243, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (239.70, 118.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (276.30, 133.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 311, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3382) [M::mem_pestat] mean and std.dev: (723.00, 801.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 379, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (322.22, 199.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693446 sequences (100000242 bp) in 2.737 CPU sec, 3.110 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284161, 16, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 270, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (307.61, 207.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (277.71, 134.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (303, 1407, 3117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8745) [M::mem_pestat] mean and std.dev: (1443.60, 1228.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11559) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 343, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (286.48, 142.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693556 reads in 184.086 CPU sec, 6.055 real sec sorting block 98, #reads = 1079217 mem = 536879365 peak = 1129381888 [M::process] read 693262 sequences (100000136 bp) in 2.290 CPU sec, 2.416 real sec... [M::mem_process_seqs] Processed 693256 reads in 128.728 CPU sec, 4.221 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284270, 12, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 285, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (304.34, 218.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (278.14, 135.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (159, 1104, 3246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9420) [M::mem_pestat] mean and std.dev: (1547.00, 2380.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 278, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623) [M::mem_pestat] mean and std.dev: (361.98, 308.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 99, #reads = 1080974 mem = 536885634 peak = 1129381888 [M::process] read 693458 sequences (100000284 bp) in 2.719 CPU sec, 3.187 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 284128, 20, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 216, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570) [M::mem_pestat] mean and std.dev: (274.52, 240.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (278.23, 135.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (142, 386, 1111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3049) [M::mem_pestat] mean and std.dev: (684.05, 690.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 250, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (269.39, 153.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693446 reads in 183.711 CPU sec, 5.991 real sec [M::mem_process_seqs] Processed 693262 reads in 129.510 CPU sec, 4.220 real sec [M::process] read 693258 sequences (100000071 bp) in 2.317 CPU sec, 2.459 real sec... sorting block 100, #reads = 1079561 mem = 536878784 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284286, 22, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 359, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1333) [M::mem_pestat] mean and std.dev: (296.12, 191.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (277.07, 134.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 483, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1666) [M::mem_pestat] mean and std.dev: (420.95, 300.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2148) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 326, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3624) [M::mem_pestat] mean and std.dev: (717.91, 803.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693654 sequences (100000095 bp) in 2.645 CPU sec, 3.124 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284394, 13, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 252, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (376.71, 391.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (279.61, 136.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 949, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5315) [M::mem_pestat] mean and std.dev: (1128.46, 898.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 209, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1451) [M::mem_pestat] mean and std.dev: (357.11, 329.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693458 reads in 183.334 CPU sec, 6.002 real sec [M::mem_process_seqs] Processed 693258 reads in 130.117 CPU sec, 4.194 real sec [M::process] read 693282 sequences (100000208 bp) in 2.482 CPU sec, 2.610 real sec... sorting block 101, #reads = 1080594 mem = 536879285 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 284632, 20, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 331, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2678) [M::mem_pestat] mean and std.dev: (601.56, 495.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (278.07, 135.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (435, 764, 2306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6048) [M::mem_pestat] mean and std.dev: (952.56, 754.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7919) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 345, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034) [M::mem_pestat] mean and std.dev: (526.20, 469.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693160 sequences (100000204 bp) in 2.846 CPU sec, 3.212 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 284171, 21, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 387, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2578) [M::mem_pestat] mean and std.dev: (624.12, 567.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 257, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (278.80, 135.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (326, 1462, 2228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6032) [M::mem_pestat] mean and std.dev: (1302.45, 938.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 405, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3040) [M::mem_pestat] mean and std.dev: (616.51, 615.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693654 reads in 185.133 CPU sec, 6.037 real sec [M::process] read 692980 sequences (100000103 bp) in 2.297 CPU sec, 2.364 real sec... [M::mem_process_seqs] Processed 693282 reads in 129.882 CPU sec, 4.211 real sec sorting block 102, #reads = 1081222 mem = 536878895 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 284249, 18, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 264, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1222) [M::mem_pestat] mean and std.dev: (352.48, 277.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (279.86, 136.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 685, 1340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3500) [M::mem_pestat] mean and std.dev: (830.53, 805.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 375, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449) [M::mem_pestat] mean and std.dev: (434.48, 299.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 103, #reads = 1079883 mem = 536877723 peak = 1129381888 [M::process] read 692714 sequences (100000088 bp) in 2.737 CPU sec, 3.142 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284324, 17, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 289, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2233) [M::mem_pestat] mean and std.dev: (426.07, 463.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (280.89, 137.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (564, 1829, 2910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7602) [M::mem_pestat] mean and std.dev: (1864.82, 1468.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9948) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 295, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (302.16, 148.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693160 reads in 183.951 CPU sec, 6.031 real sec [M::mem_process_seqs] Processed 692980 reads in 130.183 CPU sec, 4.225 real sec [M::process] read 692902 sequences (100000257 bp) in 2.514 CPU sec, 2.623 real sec... sorting block 104, #reads = 1079586 mem = 536878512 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284013, 23, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 247, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2067) [M::mem_pestat] mean and std.dev: (437.20, 429.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 260, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (282.33, 137.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 980, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3050) [M::mem_pestat] mean and std.dev: (1066.22, 740.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4028) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 354, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (549.44, 497.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3909) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693026 sequences (100000202 bp) in 2.706 CPU sec, 3.270 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284494, 15, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 361, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144) [M::mem_pestat] mean and std.dev: (370.61, 224.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (282.34, 138.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1259, 1961, 3306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7400) [M::mem_pestat] mean and std.dev: (1550.83, 1129.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 298, 1380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3678) [M::mem_pestat] mean and std.dev: (706.10, 741.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692714 reads in 184.560 CPU sec, 6.007 real sec [M::mem_process_seqs] Processed 692902 reads in 130.750 CPU sec, 4.201 real sec [M::process] read 692474 sequences (100000106 bp) in 2.567 CPU sec, 2.643 real sec... sorting block 105, #reads = 1079539 mem = 536881746 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 283888, 18, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 402, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1822) [M::mem_pestat] mean and std.dev: (468.30, 318.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (281.85, 137.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (467, 736, 2344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6098) [M::mem_pestat] mean and std.dev: (1379.33, 1023.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 360, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (307.63, 159.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699572 sequences (100000176 bp) in 2.859 CPU sec, 3.259 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284083, 26, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 349, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309) [M::mem_pestat] mean and std.dev: (340.79, 204.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 261, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (283.44, 138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1648, 2423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6079) [M::mem_pestat] mean and std.dev: (1473.04, 1124.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 267, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (279.83, 135.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693026 reads in 187.964 CPU sec, 6.100 real sec [M::mem_process_seqs] Processed 692474 reads in 133.986 CPU sec, 4.324 real sec sorting block 106, #reads = 1079790 mem = 536881017 peak = 1129381888 [M::process] read 704604 sequences (100000071 bp) in 2.716 CPU sec, 2.978 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 286415, 15, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 282, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370) [M::mem_pestat] mean and std.dev: (342.19, 268.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 237, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (262.67, 131.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (771, 1099, 8389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23625) [M::mem_pestat] mean and std.dev: (3495.93, 3604.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 31243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 256, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (236.19, 100.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 107, #reads = 1079066 mem = 536879995 peak = 1129381888 [M::process] read 703624 sequences (100000115 bp) in 2.994 CPU sec, 3.437 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 287677, 21, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 260, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (257.48, 145.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (246.68, 123.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (668, 1595, 3316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8612) [M::mem_pestat] mean and std.dev: (1942.14, 1374.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 262, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (323.52, 215.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699572 reads in 212.178 CPU sec, 6.871 real sec [M::process] read 702224 sequences (100000295 bp) in 2.266 CPU sec, 2.398 real sec... [M::mem_process_seqs] Processed 704604 reads in 148.204 CPU sec, 4.788 real sec sorting block 108, #reads = 1078643 mem = 536888331 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 287538, 24, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 233, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (284.11, 225.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (251.28, 126.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (283, 945, 2171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5947) [M::mem_pestat] mean and std.dev: (1295.70, 1514.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7835) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 261, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2054) [M::mem_pestat] mean and std.dev: (354.78, 317.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701958 sequences (100000255 bp) in 2.861 CPU sec, 3.256 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 287410, 19, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 329, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1466) [M::mem_pestat] mean and std.dev: (390.57, 269.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (255.77, 128.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (348, 559, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4620) [M::mem_pestat] mean and std.dev: (845.82, 1000.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 259, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477) [M::mem_pestat] mean and std.dev: (360.72, 260.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703624 reads in 205.151 CPU sec, 6.615 real sec [M::process] read 702636 sequences (100000255 bp) in 2.362 CPU sec, 2.520 real sec... [M::mem_process_seqs] Processed 702224 reads in 142.656 CPU sec, 4.565 real sec sorting block 109, #reads = 1082350 mem = 536881529 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 287637, 22, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 344, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (327.27, 183.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 230, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (256.79, 129.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (452, 1085, 2694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7178) [M::mem_pestat] mean and std.dev: (1797.23, 1909.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 327, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412) [M::mem_pestat] mean and std.dev: (435.92, 302.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701438 sequences (100000233 bp) in 2.899 CPU sec, 3.533 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 287775, 9, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 407, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (376.87, 190.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (255.88, 128.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 312, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2801) [M::mem_pestat] mean and std.dev: (495.73, 463.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 700792 sequences (100000115 bp) in 2.317 CPU sec, 2.333 real sec... [M::mem_process_seqs] Processed 701958 reads in 195.969 CPU sec, 6.356 real sec sorting block 110, #reads = 1083709 mem = 536881098 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 287275, 22, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 356, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234) [M::mem_pestat] mean and std.dev: (424.79, 270.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (259.10, 130.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 461, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1783) [M::mem_pestat] mean and std.dev: (442.85, 263.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 395, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (403.15, 251.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700166 sequences (100000045 bp) in 2.374 CPU sec, 2.378 real sec... [M::mem_process_seqs] Processed 701438 reads in 108.818 CPU sec, 3.634 real sec [M::mem_process_seqs] Processed 702636 reads in 280.783 CPU sec, 9.141 real sec sorting block 111, #reads = 1084464 mem = 536880397 peak = 1129381888 [M::process] read 699174 sequences (100000009 bp) in 3.509 CPU sec, 3.774 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 286985, 16, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 455, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3646) [M::mem_pestat] mean and std.dev: (682.05, 629.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 236, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (262.36, 131.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (415, 445, 2226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5848) [M::mem_pestat] mean and std.dev: (1027.56, 1263.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 263, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1928) [M::mem_pestat] mean and std.dev: (450.53, 431.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700166 reads in 105.290 CPU sec, 3.328 real sec sorting block 112, #reads = 1084099 mem = 536879988 peak = 1129381888 [M::process] read 698784 sequences (100000176 bp) in 2.846 CPU sec, 2.983 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 287300, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 279, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2073) [M::mem_pestat] mean and std.dev: (395.67, 321.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 234, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (260.35, 130.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (75, 1141, 3076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9078) [M::mem_pestat] mean and std.dev: (1933.93, 2642.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 377, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4629) [M::mem_pestat] mean and std.dev: (803.48, 898.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 286809, 19, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 243, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (225.59, 105.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (265.27, 133.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (131, 504, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2801) [M::mem_pestat] mean and std.dev: (759.26, 782.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3890) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 256, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (308.51, 200.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700792 reads in 314.396 CPU sec, 10.035 real sec [M::mem_process_seqs] Processed 699174 reads in 153.958 CPU sec, 4.951 real sec [M::process] read 699154 sequences (100000038 bp) in 2.490 CPU sec, 2.626 real sec... sorting block 113, #reads = 1084115 mem = 536880765 peak = 1129381888 [M::process] read 698338 sequences (100000020 bp) in 2.578 CPU sec, 3.401 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 286881, 12, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 213, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2062) [M::mem_pestat] mean and std.dev: (300.27, 292.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (266.88, 134.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (579, 579, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1467) [M::mem_pestat] mean and std.dev: (686.75, 351.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 270, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (466.84, 377.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699154 reads in 108.546 CPU sec, 3.524 real sec [M::process] read 698354 sequences (100000017 bp) in 2.634 CPU sec, 2.724 real sec... sorting block 114, #reads = 1085129 mem = 536880109 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 286569, 28, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 280, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (312.48, 167.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (266.95, 134.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 427, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2551) [M::mem_pestat] mean and std.dev: (497.76, 389.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 335, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2465) [M::mem_pestat] mean and std.dev: (519.23, 461.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286632, 17, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 228, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (251.75, 161.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.45, 134.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 622, 1926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5156) [M::mem_pestat] mean and std.dev: (1177.29, 1329.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6771) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 309, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1654) [M::mem_pestat] mean and std.dev: (393.26, 308.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698784 reads in 300.200 CPU sec, 9.725 real sec [M::mem_process_seqs] Processed 698338 reads in 157.327 CPU sec, 5.002 real sec [M::process] read 698100 sequences (100000290 bp) in 2.246 CPU sec, 2.291 real sec... sorting block 115, #reads = 1083465 mem = 536877918 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 286783, 17, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 184, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (217.61, 130.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.60, 135.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (481, 1148, 3242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8764) [M::mem_pestat] mean and std.dev: (1811.00, 1767.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 373, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173) [M::mem_pestat] mean and std.dev: (351.32, 190.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698178 sequences (100000112 bp) in 3.017 CPU sec, 3.233 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286885, 35, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 225, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (258.42, 147.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (269.70, 135.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (467, 1124, 3202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8672) [M::mem_pestat] mean and std.dev: (1987.00, 2082.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 279, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1754) [M::mem_pestat] mean and std.dev: (425.57, 346.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697352 sequences (100000033 bp) in 1.631 CPU sec, 1.634 real sec... [M::mem_process_seqs] Processed 698354 reads in 186.532 CPU sec, 5.933 real sec [M::mem_process_seqs] Processed 698100 reads in 127.293 CPU sec, 4.042 real sec sorting block 116, #reads = 1083426 mem = 536879288 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286311, 19, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 279, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177) [M::mem_pestat] mean and std.dev: (314.19, 195.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (269.26, 134.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (483, 1142, 2035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5139) [M::mem_pestat] mean and std.dev: (1241.39, 746.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 225, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418) [M::mem_pestat] mean and std.dev: (295.05, 220.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698404 sequences (100000093 bp) in 3.514 CPU sec, 3.763 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 286196, 6, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 292, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2398) [M::mem_pestat] mean and std.dev: (501.37, 540.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.16, 135.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 383, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1530) [M::mem_pestat] mean and std.dev: (403.43, 251.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698178 reads in 190.450 CPU sec, 6.058 real sec [M::mem_process_seqs] Processed 697352 reads in 132.145 CPU sec, 4.188 real sec [M::process] read 697534 sequences (100000110 bp) in 2.631 CPU sec, 2.734 real sec... sorting block 117, #reads = 1083212 mem = 536880958 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 285959, 13, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 239, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (281.13, 192.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.85, 135.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 730, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3193) [M::mem_pestat] mean and std.dev: (618.92, 428.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 347, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1698) [M::mem_pestat] mean and std.dev: (484.66, 420.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 118, #reads = 1083276 mem = 536876130 peak = 1129381888 [M::process] read 697866 sequences (100000272 bp) in 3.102 CPU sec, 3.410 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 286378, 15, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 260, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (212.84, 107.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (269.84, 134.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (646, 669, 2996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7696) [M::mem_pestat] mean and std.dev: (1366.20, 1257.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10046) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 301, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (282.08, 132.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698404 reads in 192.108 CPU sec, 6.263 real sec [M::mem_process_seqs] Processed 697534 reads in 134.495 CPU sec, 4.340 real sec [M::process] read 697938 sequences (100000143 bp) in 2.549 CPU sec, 2.746 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 286185, 13, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 320, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (363.26, 270.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (270.17, 136.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (294, 473, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1875) [M::mem_pestat] mean and std.dev: (395.64, 222.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2402) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 343, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1610) [M::mem_pestat] mean and std.dev: (417.20, 276.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 119, #reads = 1082051 mem = 536878167 peak = 1129381888 [M::process] read 697662 sequences (100000047 bp) in 2.869 CPU sec, 3.046 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 286240, 12, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 366, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (336.98, 161.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (270.03, 135.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 689, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3163) [M::mem_pestat] mean and std.dev: (633.09, 487.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 234, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191) [M::mem_pestat] mean and std.dev: (281.85, 172.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1530) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697866 reads in 184.716 CPU sec, 5.926 real sec [M::mem_process_seqs] Processed 697938 reads in 123.007 CPU sec, 3.876 real sec [M::process] read 697728 sequences (100000081 bp) in 2.292 CPU sec, 2.334 real sec... sorting block 120, #reads = 1083378 mem = 536878656 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 286142, 18, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 377, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2715) [M::mem_pestat] mean and std.dev: (366.52, 355.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (271.02, 135.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 561, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3443) [M::mem_pestat] mean and std.dev: (807.82, 730.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 332, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235) [M::mem_pestat] mean and std.dev: (307.77, 213.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697520 sequences (100000057 bp) in 2.387 CPU sec, 2.478 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 286122, 17, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 267, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172) [M::mem_pestat] mean and std.dev: (318.21, 247.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (269.37, 134.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 478, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2692) [M::mem_pestat] mean and std.dev: (577.33, 548.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 261, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (297.72, 145.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697472 sequences (100000265 bp) in 2.130 CPU sec, 2.128 real sec... [M::mem_process_seqs] Processed 697662 reads in 184.570 CPU sec, 5.853 real sec [M::mem_process_seqs] Processed 697728 reads in 130.562 CPU sec, 4.146 real sec sorting block 121, #reads = 1080989 mem = 536878326 peak = 1129381888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286306, 18, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 276, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (323.71, 208.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (271.18, 135.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 686, 1728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4408) [M::mem_pestat] mean and std.dev: (957.00, 684.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 325, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (486.78, 444.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697466 sequences (100000222 bp) in 2.914 CPU sec, 3.198 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 285844, 16, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 230, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (285.79, 203.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (270.52, 135.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (450, 1055, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6903) [M::mem_pestat] mean and std.dev: (1485.19, 1284.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 323, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593) [M::mem_pestat] mean and std.dev: (638.91, 763.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697520 reads in 193.222 CPU sec, 6.177 real sec sorting block 122, #reads = 1082250 mem = 536881067 peak = 1129381888 [M::process] read 697006 sequences (100000075 bp) in 2.404 CPU sec, 2.416 real sec... [M::mem_process_seqs] Processed 697472 reads in 133.419 CPU sec, 4.236 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 286385, 23, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 315, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (313.67, 184.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.77, 136.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 461, 1103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2785) [M::mem_pestat] mean and std.dev: (606.25, 554.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 317, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (349.12, 223.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 123, #reads = 1081696 mem = 536880818 peak = 1149726720 [M::process] read 696612 sequences (100000174 bp) in 3.172 CPU sec, 3.457 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285910, 13, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 361, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1932) [M::mem_pestat] mean and std.dev: (536.27, 472.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.67, 135.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (576, 1136, 2136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5256) [M::mem_pestat] mean and std.dev: (1517.77, 1078.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 295, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247) [M::mem_pestat] mean and std.dev: (415.49, 335.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697466 reads in 189.161 CPU sec, 6.046 real sec [M::mem_process_seqs] Processed 697006 reads in 128.825 CPU sec, 4.056 real sec [M::process] read 696280 sequences (100000245 bp) in 2.616 CPU sec, 2.666 real sec... sorting block 124, #reads = 1083362 mem = 536884541 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285686, 15, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 210, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (212.97, 92.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.25, 136.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1040, 1199, 1745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3155) [M::mem_pestat] mean and std.dev: (1217.15, 497.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 335, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1360) [M::mem_pestat] mean and std.dev: (400.95, 279.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696280 sequences (100000065 bp) in 3.094 CPU sec, 3.269 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285667, 24, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 306, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413) [M::mem_pestat] mean and std.dev: (475.74, 550.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.53, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (603, 1050, 2981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7737) [M::mem_pestat] mean and std.dev: (1788.00, 1941.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (328.76, 201.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696612 reads in 191.261 CPU sec, 6.090 real sec [M::mem_process_seqs] Processed 696280 reads in 132.193 CPU sec, 4.161 real sec sorting block 125, #reads = 1082133 mem = 536883103 peak = 1149726720 [M::process] read 695642 sequences (100000296 bp) in 3.146 CPU sec, 3.229 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 285096, 30, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 259, 967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2611) [M::mem_pestat] mean and std.dev: (419.28, 425.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (272.90, 134.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 1175, 2473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6663) [M::mem_pestat] mean and std.dev: (1372.39, 1062.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 318, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (278.66, 147.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695094 sequences (100000041 bp) in 2.856 CPU sec, 2.937 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 284932, 18, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 305, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (320.63, 186.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (274.22, 134.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1195, 1518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3296) [M::mem_pestat] mean and std.dev: (962.38, 584.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 289, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1604) [M::mem_pestat] mean and std.dev: (391.80, 291.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2072) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696280 reads in 193.986 CPU sec, 6.146 real sec [M::process] read 695068 sequences (100000062 bp) in 2.180 CPU sec, 2.176 real sec... sorting block 126, #reads = 1079812 mem = 536878803 peak = 1149726720 [M::mem_process_seqs] Processed 695642 reads in 134.721 CPU sec, 4.251 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284775, 18, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 325, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3273) [M::mem_pestat] mean and std.dev: (612.22, 625.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (274.07, 133.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 345, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (275.88, 198.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 362, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (312.10, 171.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 127, #reads = 1077652 mem = 536879639 peak = 1149726720 [M::process] read 694710 sequences (100000068 bp) in 3.376 CPU sec, 3.642 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284666, 32, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 497, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617) [M::mem_pestat] mean and std.dev: (507.25, 426.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (274.73, 134.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (334, 581, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2878) [M::mem_pestat] mean and std.dev: (723.55, 555.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (280.14, 148.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1414) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695094 reads in 190.696 CPU sec, 6.036 real sec [M::process] read 694262 sequences (100000263 bp) in 2.388 CPU sec, 2.399 real sec... [M::mem_process_seqs] Processed 695068 reads in 132.036 CPU sec, 4.168 real sec sorting block 128, #reads = 1078556 mem = 536877848 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284625, 26, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 322, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550) [M::mem_pestat] mean and std.dev: (331.00, 221.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (276.64, 135.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 918, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3049) [M::mem_pestat] mean and std.dev: (555.73, 547.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4051) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 321, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2066) [M::mem_pestat] mean and std.dev: (575.66, 501.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2685) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694114 sequences (100000232 bp) in 2.509 CPU sec, 2.638 real sec... [M::process] read 694280 sequences (100000155 bp) in 1.980 CPU sec, 1.981 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 284417, 16, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 229, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (287.18, 210.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (277.73, 136.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (537, 1621, 3165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8421) [M::mem_pestat] mean and std.dev: (2177.19, 2305.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 288, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2260) [M::mem_pestat] mean and std.dev: (475.08, 453.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694710 reads in 189.197 CPU sec, 6.008 real sec sorting block 129, #reads = 1078382 mem = 536879426 peak = 1149726720 [M::mem_process_seqs] Processed 694262 reads in 124.612 CPU sec, 3.953 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 284853, 37, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 277, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3275) [M::mem_pestat] mean and std.dev: (775.70, 944.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (278.06, 135.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 506, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2914) [M::mem_pestat] mean and std.dev: (556.62, 418.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 227, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292) [M::mem_pestat] mean and std.dev: (304.39, 235.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694326 sequences (100000038 bp) in 3.170 CPU sec, 3.305 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284838, 33, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 456, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2814) [M::mem_pestat] mean and std.dev: (689.19, 550.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (277.90, 136.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (348, 1397, 1903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5013) [M::mem_pestat] mean and std.dev: (1374.42, 1048.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 266, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (237.27, 75.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694114 reads in 173.825 CPU sec, 5.499 real sec sorting block 130, #reads = 1077631 mem = 536878461 peak = 1149726720 [M::process] read 694314 sequences (100000008 bp) in 2.458 CPU sec, 2.470 real sec... [M::mem_process_seqs] Processed 694280 reads in 121.678 CPU sec, 3.828 real sec sorting block 131, #reads = 1077674 mem = 536878399 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284622, 30, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 316, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (327.69, 161.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (279.11, 137.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 504, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4293) [M::mem_pestat] mean and std.dev: (587.79, 502.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1577) [M::mem_pestat] mean and std.dev: (391.78, 299.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694630 sequences (100000256 bp) in 2.846 CPU sec, 2.899 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284692, 15, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 169, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (183.26, 67.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (277.49, 136.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 736, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1638) [M::mem_pestat] mean and std.dev: (590.42, 315.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 387, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433) [M::mem_pestat] mean and std.dev: (344.11, 194.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708386 sequences (100000262 bp) in 2.290 CPU sec, 2.284 real sec... [M::mem_process_seqs] Processed 694326 reads in 175.831 CPU sec, 5.547 real sec [M::mem_process_seqs] Processed 694314 reads in 119.357 CPU sec, 3.765 real sec sorting block 132, #reads = 1077087 mem = 536877783 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285328, 29, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 267, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (303.22, 202.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (277.61, 137.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (593, 1074, 1739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4031) [M::mem_pestat] mean and std.dev: (1067.38, 785.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 306, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327) [M::mem_pestat] mean and std.dev: (393.58, 271.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 709700 sequences (100000123 bp) in 2.850 CPU sec, 2.909 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 288823, 22, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 217, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (266.64, 166.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 213, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (236.43, 115.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (544, 874, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2416) [M::mem_pestat] mean and std.dev: (731.11, 331.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 305, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1616) [M::mem_pestat] mean and std.dev: (332.18, 252.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708458 sequences (100000207 bp) in 2.370 CPU sec, 2.374 real sec... [M::mem_process_seqs] Processed 694630 reads in 187.412 CPU sec, 5.910 real sec sorting block 133, #reads = 1077496 mem = 536880002 peak = 1149726720 [M::mem_process_seqs] Processed 708386 reads in 134.633 CPU sec, 4.257 real sec [M::process] read 707354 sequences (100000297 bp) in 2.833 CPU sec, 2.906 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 289727, 19, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 250, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (205.75, 85.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 214, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (238.72, 117.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 665, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (681.89, 540.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 295, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (393.19, 337.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706654 sequences (100000258 bp) in 2.020 CPU sec, 2.026 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 289491, 16, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 226, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516) [M::mem_pestat] mean and std.dev: (294.05, 232.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (233.32, 113.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (510, 858, 2171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5493) [M::mem_pestat] mean and std.dev: (1087.73, 938.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7154) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 221, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (237.33, 117.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 708458 reads in 205.154 CPU sec, 6.500 real sec sorting block 134, #reads = 1076861 mem = 536877881 peak = 1149726720 [M::mem_process_seqs] Processed 709700 reads in 231.619 CPU sec, 7.375 real sec sorting block 135, #reads = 1075695 mem = 536879737 peak = 1149726720 [M::process] read 705706 sequences (100000282 bp) in 2.283 CPU sec, 2.360 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 289381, 11, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 266, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143) [M::mem_pestat] mean and std.dev: (414.09, 391.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 216, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (241.06, 119.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1167, 1687, 5914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15408) [M::mem_pestat] mean and std.dev: (3564.55, 3146.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20155) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 293, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (284.86, 182.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705022 sequences (100000129 bp) in 1.770 CPU sec, 1.767 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 288796, 13, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 227, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (215.19, 93.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (242.78, 120.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (382, 580, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3397) [M::mem_pestat] mean and std.dev: (684.69, 502.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4402) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 250, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2101) [M::mem_pestat] mean and std.dev: (366.55, 347.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 288987, 14, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 424, 1261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3237) [M::mem_pestat] mean and std.dev: (699.75, 670.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (245.68, 122.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 1103, 3194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8826) [M::mem_pestat] mean and std.dev: (1516.71, 1378.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11642) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 391, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2214) [M::mem_pestat] mean and std.dev: (541.51, 518.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707354 reads in 271.003 CPU sec, 8.660 real sec [M::mem_process_seqs] Processed 706654 reads in 209.327 CPU sec, 6.712 real sec sorting block 136, #reads = 1077538 mem = 536883233 peak = 1149726720 [M::process] read 703942 sequences (100000182 bp) in 2.924 CPU sec, 2.984 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 288542, 14, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 227, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (264.00, 178.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (248.33, 123.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 557, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3019) [M::mem_pestat] mean and std.dev: (770.85, 581.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (235.47, 116.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705022 reads in 107.214 CPU sec, 3.375 real sec [M::process] read 702032 sequences (100000029 bp) in 2.075 CPU sec, 2.134 real sec... [M::mem_process_seqs] Processed 705706 reads in 385.283 CPU sec, 12.285 real sec sorting block 137, #reads = 1077954 mem = 536879062 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 288276, 38, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 272, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (320.80, 231.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (250.10, 124.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 371, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3538) [M::mem_pestat] mean and std.dev: (421.50, 537.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 315, 761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1843) [M::mem_pestat] mean and std.dev: (513.25, 420.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702478 sequences (100000006 bp) in 2.243 CPU sec, 2.344 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 287426, 10, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 345, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2640) [M::mem_pestat] mean and std.dev: (623.42, 686.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 230, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (255.04, 126.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (414, 805, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3102) [M::mem_pestat] mean and std.dev: (942.00, 834.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 280, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569) [M::mem_pestat] mean and std.dev: (375.60, 263.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701638 sequences (100000136 bp) in 1.740 CPU sec, 1.739 real sec... [M::mem_process_seqs] Processed 703942 reads in 214.052 CPU sec, 6.771 real sec sorting block 138, #reads = 1077957 mem = 536876543 peak = 1149726720 [M::mem_process_seqs] Processed 702032 reads in 130.437 CPU sec, 4.159 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 287885, 29, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (396.93, 406.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 228, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (254.05, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (691, 883, 2519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6175) [M::mem_pestat] mean and std.dev: (1451.93, 1311.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 304, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1350) [M::mem_pestat] mean and std.dev: (332.76, 205.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701186 sequences (100000156 bp) in 2.804 CPU sec, 2.954 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 287808, 18, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 340, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394) [M::mem_pestat] mean and std.dev: (402.71, 333.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (257.15, 128.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (239, 846, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4340) [M::mem_pestat] mean and std.dev: (1044.56, 962.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 543, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3867) [M::mem_pestat] mean and std.dev: (778.19, 784.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700514 sequences (100000179 bp) in 2.010 CPU sec, 2.008 real sec... [M::mem_process_seqs] Processed 702478 reads in 179.785 CPU sec, 5.723 real sec sorting block 139, #reads = 1077351 mem = 536876528 peak = 1149726720 [M::mem_process_seqs] Processed 701638 reads in 118.464 CPU sec, 3.957 real sec sorting block 140, #reads = 1079535 mem = 536883141 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 287051, 17, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 414, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2806) [M::mem_pestat] mean and std.dev: (614.63, 598.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 232, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (258.15, 128.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 643, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2209) [M::mem_pestat] mean and std.dev: (674.71, 497.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 265, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (296.26, 159.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700268 sequences (100000068 bp) in 2.937 CPU sec, 3.096 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 287560, 15, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 322, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1684) [M::mem_pestat] mean and std.dev: (345.93, 306.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (260.00, 128.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1421) [M::mem_pestat] mean and std.dev: (239.58, 142.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 446, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1734) [M::mem_pestat] mean and std.dev: (430.67, 273.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699588 sequences (100000139 bp) in 1.960 CPU sec, 1.967 real sec... [M::mem_process_seqs] Processed 701186 reads in 178.580 CPU sec, 5.866 real sec [M::mem_process_seqs] Processed 700514 reads in 116.227 CPU sec, 3.677 real sec sorting block 141, #reads = 1078987 mem = 536877329 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 286981, 22, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 304, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (288.38, 135.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (260.72, 129.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 775, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2964) [M::mem_pestat] mean and std.dev: (618.60, 471.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3872) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 271, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (319.22, 167.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699546 sequences (100000094 bp) in 2.807 CPU sec, 2.967 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 287240, 23, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 245, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977) [M::mem_pestat] mean and std.dev: (270.90, 159.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (262.87, 130.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (288, 831, 2149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5871) [M::mem_pestat] mean and std.dev: (971.95, 1120.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 384, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2423) [M::mem_pestat] mean and std.dev: (504.55, 463.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699312 sequences (100000067 bp) in 2.000 CPU sec, 1.993 real sec... [M::mem_process_seqs] Processed 700268 reads in 172.730 CPU sec, 5.621 real sec [M::mem_process_seqs] Processed 699588 reads in 109.328 CPU sec, 3.528 real sec sorting block 142, #reads = 1078950 mem = 536885253 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 286858, 16, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 294, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (276.52, 107.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (262.76, 130.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 1252, 3311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8669) [M::mem_pestat] mean and std.dev: (2109.62, 1917.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 390, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (405.24, 259.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698682 sequences (100000036 bp) in 2.667 CPU sec, 2.786 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286613, 23, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 336, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (369.61, 240.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (264.73, 131.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (409, 966, 1643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4111) [M::mem_pestat] mean and std.dev: (1082.32, 1025.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 218, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2297) [M::mem_pestat] mean and std.dev: (501.71, 582.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699546 reads in 166.967 CPU sec, 5.370 real sec [M::process] read 698190 sequences (100000063 bp) in 2.026 CPU sec, 2.030 real sec... sorting block 143, #reads = 1080041 mem = 536880454 peak = 1149726720 [M::mem_process_seqs] Processed 699312 reads in 111.147 CPU sec, 3.691 real sec sorting block 144, #reads = 1079668 mem = 536878417 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 286399, 22, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 267, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2136) [M::mem_pestat] mean and std.dev: (494.36, 524.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (264.58, 131.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 608, 2046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5728) [M::mem_pestat] mean and std.dev: (863.80, 924.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 255, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (263.31, 160.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698654 sequences (100000288 bp) in 2.715 CPU sec, 2.849 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 286756, 10, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 360, 841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2009) [M::mem_pestat] mean and std.dev: (460.53, 389.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 241, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (265.99, 131.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 1271, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3563) [M::mem_pestat] mean and std.dev: (825.33, 579.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 523, 872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112) [M::mem_pestat] mean and std.dev: (510.51, 407.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698556 sequences (100000110 bp) in 1.990 CPU sec, 1.990 real sec... [M::mem_process_seqs] Processed 698682 reads in 175.076 CPU sec, 5.732 real sec [M::mem_process_seqs] Processed 698190 reads in 110.656 CPU sec, 3.564 real sec sorting block 145, #reads = 1079248 mem = 536877714 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 286349, 15, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 340, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2919) [M::mem_pestat] mean and std.dev: (729.97, 846.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (263.75, 130.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (406, 524, 3042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8314) [M::mem_pestat] mean and std.dev: (1887.60, 2454.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 311, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (342.61, 185.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698106 sequences (100000101 bp) in 2.932 CPU sec, 3.094 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 286617, 30, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 317, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712) [M::mem_pestat] mean and std.dev: (432.97, 350.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2215) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (265.61, 132.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 541, 1318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3320) [M::mem_pestat] mean and std.dev: (817.48, 679.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 204, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (224.60, 87.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698654 reads in 165.611 CPU sec, 5.340 real sec [M::mem_process_seqs] Processed 698556 reads in 109.739 CPU sec, 3.472 real sec [M::process] read 697520 sequences (100000101 bp) in 2.471 CPU sec, 2.537 real sec... sorting block 146, #reads = 1079604 mem = 536883081 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 286383, 16, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 322, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (343.04, 212.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (266.89, 132.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (294, 1178, 3342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9438) [M::mem_pestat] mean and std.dev: (1836.94, 2089.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 346, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (349.34, 233.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697212 sequences (100000003 bp) in 3.187 CPU sec, 3.395 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285793, 14, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 304, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688) [M::mem_pestat] mean and std.dev: (470.30, 398.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (269.96, 135.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (525, 569, 2095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5235) [M::mem_pestat] mean and std.dev: (829.25, 719.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 266, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3043) [M::mem_pestat] mean and std.dev: (552.71, 677.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698106 reads in 161.237 CPU sec, 5.141 real sec sorting block 147, #reads = 1078980 mem = 536878934 peak = 1149726720 [M::process] read 696872 sequences (100000276 bp) in 2.709 CPU sec, 2.828 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 285937, 16, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 268, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2125) [M::mem_pestat] mean and std.dev: (405.26, 303.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (270.75, 136.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 557, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3710) [M::mem_pestat] mean and std.dev: (672.00, 561.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 236, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2344) [M::mem_pestat] mean and std.dev: (453.55, 506.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3070) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697212 reads in 91.571 CPU sec, 2.891 real sec [M::process] read 696680 sequences (100000044 bp) in 2.180 CPU sec, 2.234 real sec... sorting block 148, #reads = 1079855 mem = 536882620 peak = 1149726720 [M::mem_process_seqs] Processed 697520 reads in 236.802 CPU sec, 7.494 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 285814, 15, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 291, 1452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3988) [M::mem_pestat] mean and std.dev: (738.52, 886.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (272.84, 137.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 758, 2460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6624) [M::mem_pestat] mean and std.dev: (1048.00, 872.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 314, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (324.16, 156.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696508 sequences (100000204 bp) in 2.694 CPU sec, 2.828 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 285708, 11, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (279.78, 156.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (273.16, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 166, 1593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4699) [M::mem_pestat] mean and std.dev: (672.00, 869.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 267, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283) [M::mem_pestat] mean and std.dev: (354.83, 255.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696872 reads in 180.461 CPU sec, 5.713 real sec sorting block 149, #reads = 1079735 mem = 536880258 peak = 1149726720 [M::process] read 695914 sequences (100000001 bp) in 2.301 CPU sec, 2.386 real sec... [M::process] read 695390 sequences (100000059 bp) in 2.029 CPU sec, 2.031 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285156, 22, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 287, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469) [M::mem_pestat] mean and std.dev: (312.50, 216.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.70, 136.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (288, 754, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2664) [M::mem_pestat] mean and std.dev: (718.50, 518.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 338, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (324.74, 164.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695914 reads in 86.317 CPU sec, 2.807 real sec sorting block 150, #reads = 1078682 mem = 536879010 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285575, 24, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 394, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4246) [M::mem_pestat] mean and std.dev: (638.19, 647.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (273.14, 137.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (511, 1335, 4819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13435) [M::mem_pestat] mean and std.dev: (2852.04, 3552.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 277, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (263.57, 127.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696508 reads in 178.138 CPU sec, 6.587 real sec [M::mem_process_seqs] Processed 696680 reads in 342.148 CPU sec, 11.770 real sec [M::process] read 695690 sequences (100000205 bp) in 2.541 CPU sec, 2.690 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285348, 29, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 234, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1403) [M::mem_pestat] mean and std.dev: (376.00, 317.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (275.61, 137.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (568, 1728, 2467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6265) [M::mem_pestat] mean and std.dev: (1754.59, 1254.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 354, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (353.17, 221.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 151, #reads = 1079187 mem = 536880144 peak = 1149726720 [M::process] read 695188 sequences (100000179 bp) in 2.387 CPU sec, 2.494 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284839, 24, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 492, 2647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7451) [M::mem_pestat] mean and std.dev: (1579.59, 1780.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (275.02, 137.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (526, 1231, 4001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10951) [M::mem_pestat] mean and std.dev: (2263.58, 2392.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 248, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (255.20, 117.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695390 reads in 181.347 CPU sec, 5.740 real sec [M::mem_process_seqs] Processed 695690 reads in 109.411 CPU sec, 3.463 real sec sorting block 152, #reads = 1080897 mem = 536881475 peak = 1149726720 [M::process] read 695040 sequences (100000201 bp) in 2.522 CPU sec, 2.670 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 284774, 28, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 253, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (308.44, 193.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (277.03, 138.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (424, 672, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3157) [M::mem_pestat] mean and std.dev: (716.08, 543.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 308, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (326.85, 157.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694902 sequences (100000016 bp) in 2.397 CPU sec, 2.491 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285307, 17, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 300, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (357.35, 278.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (278.13, 138.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (435, 839, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2928) [M::mem_pestat] mean and std.dev: (740.25, 547.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3759) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 382, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (330.00, 159.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695188 reads in 164.981 CPU sec, 5.251 real sec [M::mem_process_seqs] Processed 695040 reads in 109.716 CPU sec, 3.468 real sec [M::process] read 694732 sequences (100000126 bp) in 2.303 CPU sec, 2.409 real sec... sorting block 153, #reads = 1078538 mem = 536879954 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 284962, 19, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 303, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2513) [M::mem_pestat] mean and std.dev: (474.92, 494.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (277.48, 138.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (466, 513, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2665) [M::mem_pestat] mean and std.dev: (517.60, 334.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 284, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (331.42, 179.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694350 sequences (100000151 bp) in 2.703 CPU sec, 2.832 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285012, 15, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 320, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (306.17, 133.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (277.90, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 391, 1171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2995) [M::mem_pestat] mean and std.dev: (649.60, 623.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 351, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2300) [M::mem_pestat] mean and std.dev: (511.59, 470.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694902 reads in 163.431 CPU sec, 5.202 real sec sorting block 154, #reads = 1079427 mem = 536879846 peak = 1149726720 [M::mem_process_seqs] Processed 694732 reads in 109.143 CPU sec, 3.446 real sec [M::process] read 694626 sequences (100000063 bp) in 2.078 CPU sec, 2.127 real sec... sorting block 155, #reads = 1078663 mem = 536877985 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 285057, 20, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 249, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172) [M::mem_pestat] mean and std.dev: (434.89, 386.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (281.14, 141.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 747, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020) [M::mem_pestat] mean and std.dev: (641.53, 620.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 353, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (339.69, 161.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702926 sequences (100000124 bp) in 2.761 CPU sec, 2.884 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 284501, 15, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 351, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2164) [M::mem_pestat] mean and std.dev: (537.86, 529.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (279.21, 140.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (227, 547, 1261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329) [M::mem_pestat] mean and std.dev: (751.33, 674.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 279, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (324.21, 184.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703630 sequences (100000128 bp) in 1.820 CPU sec, 1.817 real sec... [M::mem_process_seqs] Processed 694350 reads in 179.479 CPU sec, 5.707 real sec [M::mem_process_seqs] Processed 694626 reads in 121.509 CPU sec, 3.957 real sec sorting block 156, #reads = 1077056 mem = 536886510 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 287016, 16, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 275, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (300.90, 216.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 226, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (251.07, 124.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 499, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2965) [M::mem_pestat] mean and std.dev: (670.20, 612.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 240, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166) [M::mem_pestat] mean and std.dev: (335.06, 342.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702420 sequences (100000117 bp) in 2.819 CPU sec, 2.986 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 287533, 21, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 224, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (284.10, 155.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (250.03, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 883, 1587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4175) [M::mem_pestat] mean and std.dev: (872.11, 749.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 288, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (350.31, 254.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701276 sequences (100000167 bp) in 1.920 CPU sec, 1.923 real sec... [M::mem_process_seqs] Processed 702926 reads in 192.268 CPU sec, 6.219 real sec [M::mem_process_seqs] Processed 703630 reads in 115.873 CPU sec, 3.764 real sec sorting block 157, #reads = 1066374 mem = 536888863 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 287541, 19, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 335, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3072) [M::mem_pestat] mean and std.dev: (538.00, 503.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 228, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (253.65, 126.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (786, 1191, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3807) [M::mem_pestat] mean and std.dev: (1067.82, 645.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4814) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 331, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1487) [M::mem_pestat] mean and std.dev: (409.61, 335.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700298 sequences (100000096 bp) in 2.476 CPU sec, 2.576 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 287606, 23, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 338, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593) [M::mem_pestat] mean and std.dev: (429.91, 398.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (258.16, 128.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (88, 378, 2650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7774) [M::mem_pestat] mean and std.dev: (1199.48, 1517.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 269, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1470) [M::mem_pestat] mean and std.dev: (375.92, 303.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700336 sequences (100000162 bp) in 1.910 CPU sec, 1.909 real sec... [M::mem_process_seqs] Processed 702420 reads in 166.495 CPU sec, 5.370 real sec sorting block 158, #reads = 1082426 mem = 536878700 peak = 1149726720 [M::mem_process_seqs] Processed 701276 reads in 104.669 CPU sec, 3.388 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 287427, 11, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 270, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (262.12, 149.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 236, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (261.87, 131.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 933, 2644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7220) [M::mem_pestat] mean and std.dev: (1375.36, 1295.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 344, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2300) [M::mem_pestat] mean and std.dev: (439.10, 371.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 159, #reads = 1084759 mem = 536876684 peak = 1149726720 [M::process] read 699914 sequences (100000212 bp) in 2.539 CPU sec, 2.641 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 286839, 12, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 270, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (310.32, 188.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (261.41, 130.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 407, 1993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5689) [M::mem_pestat] mean and std.dev: (417.10, 556.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7537) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 267, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156) [M::mem_pestat] mean and std.dev: (310.97, 221.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700298 reads in 158.112 CPU sec, 5.100 real sec [M::process] read 699526 sequences (100000047 bp) in 1.920 CPU sec, 1.922 real sec... [M::mem_process_seqs] Processed 700336 reads in 104.988 CPU sec, 3.404 real sec sorting block 160, #reads = 1084268 mem = 536879286 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 286905, 28, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 277, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3549) [M::mem_pestat] mean and std.dev: (573.66, 577.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (262.93, 131.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (524, 1218, 3412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9188) [M::mem_pestat] mean and std.dev: (1833.71, 2086.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12076) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 425, 2190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6172) [M::mem_pestat] mean and std.dev: (1153.36, 1308.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699150 sequences (100000128 bp) in 2.849 CPU sec, 3.013 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 287165, 25, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 333, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1816) [M::mem_pestat] mean and std.dev: (498.71, 477.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (263.83, 131.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 1135, 2396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6786) [M::mem_pestat] mean and std.dev: (1211.25, 1014.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8981) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 371, 1112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2936) [M::mem_pestat] mean and std.dev: (551.90, 536.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699914 reads in 158.153 CPU sec, 5.110 real sec [M::mem_process_seqs] Processed 699526 reads in 103.764 CPU sec, 3.280 real sec [M::process] read 698624 sequences (100000125 bp) in 2.057 CPU sec, 2.106 real sec... sorting block 161, #reads = 1083429 mem = 536887022 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286840, 22, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 567, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3485) [M::mem_pestat] mean and std.dev: (862.50, 789.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (264.56, 132.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 632, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (475, 820) [M::mem_pestat] mean and std.dev: (643.06, 48.07) [M::mem_pestat] low and high boundaries for proper pairs: (406, 889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 278, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (264.07, 126.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698214 sequences (100000102 bp) in 2.666 CPU sec, 2.782 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 286597, 20, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 313, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (400.40, 358.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (265.59, 131.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (387, 425, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3276) [M::mem_pestat] mean and std.dev: (788.16, 716.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 277, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1237) [M::mem_pestat] mean and std.dev: (305.64, 220.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699150 reads in 156.355 CPU sec, 4.973 real sec [M::process] read 698038 sequences (100000040 bp) in 2.118 CPU sec, 2.158 real sec... [M::mem_process_seqs] Processed 698624 reads in 104.641 CPU sec, 3.463 real sec sorting block 162, #reads = 1083497 mem = 536882014 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 286495, 15, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1929) [M::mem_pestat] mean and std.dev: (508.42, 472.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (266.92, 132.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (376, 665, 1850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4798) [M::mem_pestat] mean and std.dev: (911.86, 723.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 396, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2595) [M::mem_pestat] mean and std.dev: (711.96, 734.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 163, #reads = 1084369 mem = 536877824 peak = 1149726720 [M::process] read 697626 sequences (100000088 bp) in 2.279 CPU sec, 2.386 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 286004, 20, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 260, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2198) [M::mem_pestat] mean and std.dev: (407.06, 343.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (266.75, 132.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (489, 809, 2347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6063) [M::mem_pestat] mean and std.dev: (1410.95, 1286.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 318, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (318.15, 230.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697126 sequences (100000213 bp) in 1.680 CPU sec, 1.673 real sec... [M::mem_process_seqs] Processed 698214 reads in 163.211 CPU sec, 5.373 real sec [M::mem_process_seqs] Processed 698038 reads in 104.788 CPU sec, 3.398 real sec sorting block 164, #reads = 1082220 mem = 536878501 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 286240, 18, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 254, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1989) [M::mem_pestat] mean and std.dev: (407.51, 376.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (268.87, 133.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (391, 1201, 3234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8920) [M::mem_pestat] mean and std.dev: (1537.44, 1254.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 341, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1639) [M::mem_pestat] mean and std.dev: (417.86, 327.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696904 sequences (100000210 bp) in 2.942 CPU sec, 3.148 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 286400, 23, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746) [M::mem_pestat] mean and std.dev: (346.91, 382.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (270.83, 134.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 412, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2932) [M::mem_pestat] mean and std.dev: (683.82, 652.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 260, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976) [M::mem_pestat] mean and std.dev: (347.38, 301.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697626 reads in 156.446 CPU sec, 5.046 real sec [M::mem_process_seqs] Processed 697126 reads in 102.912 CPU sec, 3.258 real sec [M::process] read 697256 sequences (100000196 bp) in 2.093 CPU sec, 2.148 real sec... sorting block 165, #reads = 1082951 mem = 536881902 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 285855, 11, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 321, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2501) [M::mem_pestat] mean and std.dev: (519.69, 544.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (270.29, 133.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (589, 895, 5788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16186) [M::mem_pestat] mean and std.dev: (2724.27, 3082.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 301, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (582.11, 542.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696836 sequences (100000268 bp) in 2.381 CPU sec, 2.501 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286304, 18, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 498, 1385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3637) [M::mem_pestat] mean and std.dev: (593.44, 556.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.20, 134.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 1254, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5476) [M::mem_pestat] mean and std.dev: (1451.06, 1467.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (310.29, 153.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696904 reads in 153.522 CPU sec, 4.895 real sec [M::process] read 697098 sequences (100000155 bp) in 1.974 CPU sec, 1.999 real sec... [M::mem_process_seqs] Processed 697256 reads in 102.458 CPU sec, 3.379 real sec sorting block 166, #reads = 1082993 mem = 536878375 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285903, 11, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 320, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354) [M::mem_pestat] mean and std.dev: (326.10, 234.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.11, 135.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 744, 1632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3634) [M::mem_pestat] mean and std.dev: (1016.09, 751.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 323, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146) [M::mem_pestat] mean and std.dev: (322.91, 188.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698004 sequences (100000099 bp) in 2.535 CPU sec, 2.663 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 286501, 12, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 445, 1788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4916) [M::mem_pestat] mean and std.dev: (904.38, 976.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (271.38, 134.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (338, 958, 9992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 29300) [M::mem_pestat] mean and std.dev: (3176.17, 4030.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 38954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 290, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381) [M::mem_pestat] mean and std.dev: (362.14, 229.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696836 reads in 154.197 CPU sec, 5.038 real sec [M::process] read 697586 sequences (100000123 bp) in 1.941 CPU sec, 1.947 real sec... sorting block 167, #reads = 1082738 mem = 536880189 peak = 1149726720 [M::mem_process_seqs] Processed 697098 reads in 100.966 CPU sec, 3.413 real sec sorting block 168, #reads = 1082755 mem = 536882160 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 286376, 9, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 259, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (282.86, 179.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 241, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (265.63, 131.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 275, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (341.10, 205.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696922 sequences (100000140 bp) in 2.443 CPU sec, 2.554 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 286521, 14, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 267, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (259.91, 137.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 242, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (268.03, 133.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 822, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3718) [M::mem_pestat] mean and std.dev: (700.85, 589.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4885) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 389, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4462) [M::mem_pestat] mean and std.dev: (639.87, 690.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5892) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698004 reads in 160.843 CPU sec, 5.332 real sec [M::process] read 697330 sequences (100000030 bp) in 1.910 CPU sec, 1.910 real sec... [M::mem_process_seqs] Processed 697586 reads in 104.114 CPU sec, 3.290 real sec sorting block 169, #reads = 1082264 mem = 536878399 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 286607, 15, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 385, 928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2444) [M::mem_pestat] mean and std.dev: (408.69, 366.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (270.79, 134.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (495, 538, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2751) [M::mem_pestat] mean and std.dev: (937.40, 871.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 319, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1996) [M::mem_pestat] mean and std.dev: (473.95, 396.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696988 sequences (100000266 bp) in 2.843 CPU sec, 3.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285998, 15, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 256, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1679) [M::mem_pestat] mean and std.dev: (436.48, 393.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (270.02, 134.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (159, 699, 2164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6174) [M::mem_pestat] mean and std.dev: (1044.71, 1160.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 279, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1661) [M::mem_pestat] mean and std.dev: (387.68, 317.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696922 reads in 162.757 CPU sec, 5.390 real sec [M::process] read 696158 sequences (100000062 bp) in 1.967 CPU sec, 1.986 real sec... [M::mem_process_seqs] Processed 697330 reads in 106.900 CPU sec, 3.630 real sec sorting block 170, #reads = 1082510 mem = 536884300 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285320, 13, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 247, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (223.19, 93.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (271.34, 135.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (753, 876, 2344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5526) [M::mem_pestat] mean and std.dev: (1232.75, 934.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7117) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 273, 947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2507) [M::mem_pestat] mean and std.dev: (495.22, 535.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695996 sequences (100000216 bp) in 2.402 CPU sec, 2.524 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 285526, 11, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 325, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618) [M::mem_pestat] mean and std.dev: (419.04, 310.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.56, 135.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (132, 185, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1758) [M::mem_pestat] mean and std.dev: (396.64, 347.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 235, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428) [M::mem_pestat] mean and std.dev: (308.29, 209.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1844) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695754 sequences (100000073 bp) in 1.900 CPU sec, 1.899 real sec... [M::mem_process_seqs] Processed 696988 reads in 167.724 CPU sec, 5.583 real sec sorting block 171, #reads = 1080179 mem = 536880741 peak = 1149726720 [M::mem_process_seqs] Processed 696158 reads in 105.097 CPU sec, 3.423 real sec sorting block 172, #reads = 1080669 mem = 536878193 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 285666, 24, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 328, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (281.07, 129.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (274.16, 136.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 689, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505) [M::mem_pestat] mean and std.dev: (668.95, 440.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 299, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1511) [M::mem_pestat] mean and std.dev: (376.69, 277.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695572 sequences (100000028 bp) in 2.691 CPU sec, 2.837 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 285510, 29, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 374, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2341) [M::mem_pestat] mean and std.dev: (504.44, 488.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.37, 137.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (647, 1129, 3562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9392) [M::mem_pestat] mean and std.dev: (1892.86, 1970.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 327, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507) [M::mem_pestat] mean and std.dev: (384.41, 251.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695996 reads in 160.827 CPU sec, 5.193 real sec [M::process] read 695632 sequences (100000063 bp) in 1.958 CPU sec, 1.953 real sec... [M::mem_process_seqs] Processed 695754 reads in 105.273 CPU sec, 3.594 real sec sorting block 173, #reads = 1080588 mem = 536880841 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 285151, 25, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 304, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (376.95, 275.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.52, 137.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 1133, 1431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3729) [M::mem_pestat] mean and std.dev: (893.05, 746.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 244, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (234.76, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695142 sequences (100000138 bp) in 2.550 CPU sec, 2.659 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 284690, 22, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 420, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2376) [M::mem_pestat] mean and std.dev: (492.92, 376.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3069) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.94, 136.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 1333, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5291) [M::mem_pestat] mean and std.dev: (1106.20, 1081.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 243, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (269.31, 174.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695310 sequences (100000029 bp) in 1.920 CPU sec, 1.926 real sec... [M::mem_process_seqs] Processed 695572 reads in 167.781 CPU sec, 5.593 real sec [M::mem_process_seqs] Processed 695632 reads in 106.281 CPU sec, 3.462 real sec sorting block 174, #reads = 1081272 mem = 536877890 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285076, 20, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 262, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1607) [M::mem_pestat] mean and std.dev: (386.82, 377.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (276.10, 137.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (255, 752, 1545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4125) [M::mem_pestat] mean and std.dev: (918.50, 957.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 247, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (289.10, 162.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696100 sequences (100000087 bp) in 2.536 CPU sec, 2.639 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284910, 18, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 342, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (369.65, 244.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (276.34, 138.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (255, 895, 1770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4800) [M::mem_pestat] mean and std.dev: (1138.65, 1306.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 290, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (292.02, 177.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695742 sequences (100000050 bp) in 1.950 CPU sec, 1.951 real sec... [M::mem_process_seqs] Processed 695142 reads in 170.136 CPU sec, 5.491 real sec [M::mem_process_seqs] Processed 695310 reads in 107.575 CPU sec, 3.406 real sec sorting block 175, #reads = 1079500 mem = 536880156 peak = 1149726720 sorting block 176, #reads = 1079558 mem = 536878974 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 286028, 21, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 264, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (273.35, 148.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.93, 136.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (341, 1150, 1510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3848) [M::mem_pestat] mean and std.dev: (1031.50, 787.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5017) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 282, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (272.53, 115.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694736 sequences (100000114 bp) in 2.558 CPU sec, 2.664 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285059, 9, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 297, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (320.13, 187.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (273.22, 136.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 353, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1588) [M::mem_pestat] mean and std.dev: (402.23, 296.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694924 sequences (100000165 bp) in 1.940 CPU sec, 1.939 real sec... [M::mem_process_seqs] Processed 696100 reads in 172.577 CPU sec, 5.519 real sec [M::mem_process_seqs] Processed 695742 reads in 109.750 CPU sec, 3.558 real sec sorting block 177, #reads = 1079343 mem = 536877098 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284669, 16, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 373, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2736) [M::mem_pestat] mean and std.dev: (561.29, 470.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (276.61, 137.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (106, 602, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4330) [M::mem_pestat] mean and std.dev: (969.50, 1116.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5738) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 303, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (277.44, 144.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694592 sequences (100000075 bp) in 2.597 CPU sec, 2.695 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284609, 24, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 288, 1203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301) [M::mem_pestat] mean and std.dev: (442.49, 581.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (276.10, 136.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (421, 1216, 2105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5473) [M::mem_pestat] mean and std.dev: (1148.43, 1076.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7157) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 288, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629) [M::mem_pestat] mean and std.dev: (334.65, 247.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694760 sequences (100000030 bp) in 1.986 CPU sec, 1.987 real sec... [M::mem_process_seqs] Processed 694736 reads in 173.865 CPU sec, 5.594 real sec [M::mem_process_seqs] Processed 694924 reads in 110.024 CPU sec, 3.481 real sec sorting block 178, #reads = 1078676 mem = 536877290 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 284483, 24, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 226, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142) [M::mem_pestat] mean and std.dev: (304.43, 249.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (276.74, 136.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 460, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3184) [M::mem_pestat] mean and std.dev: (510.95, 375.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4154) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (365.00, 274.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694942 sequences (100000268 bp) in 2.644 CPU sec, 2.730 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284757, 16, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 379, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2332) [M::mem_pestat] mean and std.dev: (479.28, 411.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (276.31, 137.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 1014, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5090) [M::mem_pestat] mean and std.dev: (1264.31, 1332.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 328, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (356.40, 190.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1383) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694210 sequences (100000258 bp) in 1.950 CPU sec, 1.950 real sec... [M::mem_process_seqs] Processed 694592 reads in 170.988 CPU sec, 5.426 real sec [M::mem_process_seqs] Processed 694760 reads in 111.971 CPU sec, 3.542 real sec sorting block 179, #reads = 1077967 mem = 536882208 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284702, 21, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 214, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (271.83, 184.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (275.81, 137.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 887, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4332) [M::mem_pestat] mean and std.dev: (1043.85, 802.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5628) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 257, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (314.13, 269.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694230 sequences (100000165 bp) in 2.557 CPU sec, 2.640 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284656, 18, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2421) [M::mem_pestat] mean and std.dev: (319.78, 377.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (278.03, 138.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (438, 675, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2169) [M::mem_pestat] mean and std.dev: (663.06, 386.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 288, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1664) [M::mem_pestat] mean and std.dev: (393.05, 304.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695448 sequences (100000190 bp) in 2.000 CPU sec, 1.995 real sec... [M::mem_process_seqs] Processed 694942 reads in 178.306 CPU sec, 5.711 real sec sorting block 180, #reads = 1076537 mem = 536876788 peak = 1149726720 [M::mem_process_seqs] Processed 694210 reads in 111.668 CPU sec, 3.535 real sec sorting block 181, #reads = 1076410 mem = 536879676 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 284705, 16, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 361, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550) [M::mem_pestat] mean and std.dev: (428.14, 319.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 253, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (278.43, 138.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 356, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2620) [M::mem_pestat] mean and std.dev: (454.73, 442.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 299, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207) [M::mem_pestat] mean and std.dev: (318.56, 178.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 709064 sequences (100000248 bp) in 2.575 CPU sec, 2.643 real sec... [M::process] read 711436 sequences (100000130 bp) in 2.040 CPU sec, 2.041 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 284417, 22, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 272, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (263.33, 128.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (274.66, 137.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 571, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3902) [M::mem_pestat] mean and std.dev: (897.00, 910.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5109) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 257, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (289.05, 156.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694230 reads in 193.675 CPU sec, 6.269 real sec [M::mem_process_seqs] Processed 695448 reads in 130.279 CPU sec, 4.158 real sec sorting block 182, #reads = 1072273 mem = 536880527 peak = 1149726720 [M::process] read 707326 sequences (100000205 bp) in 2.579 CPU sec, 2.662 real sec... [M::process] read 704856 sequences (100000054 bp) in 2.020 CPU sec, 2.022 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 290326, 25, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 234, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (263.07, 176.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (230.93, 113.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 642, 2026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5186) [M::mem_pestat] mean and std.dev: (924.05, 1097.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6766) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 233, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (258.78, 157.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 711436 reads in 107.477 CPU sec, 3.445 real sec sorting block 183, #reads = 1068124 mem = 536883808 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 289397, 21, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 214, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1379) [M::mem_pestat] mean and std.dev: (295.41, 261.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (234.46, 115.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 918, 4011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10787) [M::mem_pestat] mean and std.dev: (2189.10, 2683.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14175) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 294, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (314.53, 172.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 289617, 10, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 238, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (303.74, 228.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (242.22, 120.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (496, 1855, 2215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5653) [M::mem_pestat] mean and std.dev: (1209.88, 777.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 298, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (264.35, 135.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704348 sequences (100000168 bp) in 2.281 CPU sec, 2.284 real sec... [M::mem_process_seqs] Processed 709064 reads in 305.606 CPU sec, 9.734 real sec [M::mem_process_seqs] Processed 707326 reads in 157.790 CPU sec, 5.097 real sec sorting block 184, #reads = 1074720 mem = 536878473 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 288435, 17, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 440, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4362) [M::mem_pestat] mean and std.dev: (725.91, 990.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 223, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (249.40, 125.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 597, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3330) [M::mem_pestat] mean and std.dev: (900.94, 945.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 276, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1366) [M::mem_pestat] mean and std.dev: (410.00, 325.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703166 sequences (100000209 bp) in 2.764 CPU sec, 2.906 real sec... [M::process] read 702434 sequences (100000084 bp) in 1.940 CPU sec, 1.936 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 288123, 21, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 513, 1473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3985) [M::mem_pestat] mean and std.dev: (735.73, 652.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (250.26, 124.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 783, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3397) [M::mem_pestat] mean and std.dev: (819.48, 609.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 317, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (314.10, 183.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704856 reads in 186.670 CPU sec, 5.937 real sec sorting block 185, #reads = 1077696 mem = 536877696 peak = 1149726720 [M::mem_process_seqs] Processed 704348 reads in 122.494 CPU sec, 4.216 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 287718, 15, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 313, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (340.59, 204.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 227, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (253.87, 127.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (638, 864, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3209) [M::mem_pestat] mean and std.dev: (1064.64, 819.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 348, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296) [M::mem_pestat] mean and std.dev: (348.25, 237.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701382 sequences (100000130 bp) in 2.561 CPU sec, 2.685 real sec... [M::process] read 700488 sequences (100000079 bp) in 1.990 CPU sec, 1.990 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 287640, 12, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 294, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1695) [M::mem_pestat] mean and std.dev: (366.02, 237.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (255.20, 127.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (891, 1026, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (433, 1578) [M::mem_pestat] mean and std.dev: (930.20, 215.57) [M::mem_pestat] low and high boundaries for proper pairs: (68, 1807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 310, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (362.83, 252.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703166 reads in 187.798 CPU sec, 6.285 real sec sorting block 186, #reads = 1076015 mem = 536879289 peak = 1149726720 [M::mem_process_seqs] Processed 702434 reads in 118.277 CPU sec, 3.870 real sec sorting block 187, #reads = 1077099 mem = 536880671 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 287491, 25, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 326, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1736) [M::mem_pestat] mean and std.dev: (364.65, 313.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (257.44, 128.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (288, 880, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2892) [M::mem_pestat] mean and std.dev: (608.95, 449.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (295.32, 148.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700410 sequences (100000014 bp) in 2.586 CPU sec, 2.680 real sec... [M::process] read 700074 sequences (100000117 bp) in 1.960 CPU sec, 1.956 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 287259, 10, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 272, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427) [M::mem_pestat] mean and std.dev: (332.50, 282.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (261.60, 130.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 5105, 8581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25555) [M::mem_pestat] mean and std.dev: (4063.10, 4092.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 34042) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 297, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (323.38, 183.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701382 reads in 182.866 CPU sec, 5.911 real sec [M::mem_process_seqs] Processed 700488 reads in 118.139 CPU sec, 3.755 real sec sorting block 188, #reads = 1078805 mem = 536881301 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 287170, 20, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 303, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224) [M::mem_pestat] mean and std.dev: (373.00, 237.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (262.81, 131.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 819, 2321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5701) [M::mem_pestat] mean and std.dev: (1142.45, 907.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 309, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (353.64, 250.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698892 sequences (100000122 bp) in 2.547 CPU sec, 2.637 real sec... [M::process] read 698742 sequences (100000015 bp) in 2.010 CPU sec, 2.004 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 286962, 15, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 403, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327) [M::mem_pestat] mean and std.dev: (429.31, 252.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (263.62, 131.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (320, 948, 1780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4700) [M::mem_pestat] mean and std.dev: (1112.27, 1029.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 377, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2736) [M::mem_pestat] mean and std.dev: (608.27, 557.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700410 reads in 179.309 CPU sec, 5.689 real sec [M::mem_process_seqs] Processed 700074 reads in 116.428 CPU sec, 3.690 real sec sorting block 189, #reads = 1077904 mem = 536881319 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 286584, 12, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 241, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (256.31, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (266.12, 133.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 929, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3319) [M::mem_pestat] mean and std.dev: (558.00, 452.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 289, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (300.74, 128.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697668 sequences (100000087 bp) in 2.560 CPU sec, 2.655 real sec... [M::process] read 697570 sequences (100000170 bp) in 1.970 CPU sec, 1.971 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 286510, 23, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 227, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (313.40, 205.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (267.70, 134.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (301, 327, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2221) [M::mem_pestat] mean and std.dev: (453.53, 445.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 275, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437) [M::mem_pestat] mean and std.dev: (400.02, 372.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698892 reads in 181.718 CPU sec, 6.056 real sec sorting block 190, #reads = 1076477 mem = 536877737 peak = 1149726720 [M::mem_process_seqs] Processed 698742 reads in 115.235 CPU sec, 3.758 real sec sorting block 191, #reads = 1076722 mem = 536879305 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 286457, 17, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 310, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (346.48, 260.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (269.25, 133.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (508, 1520, 9410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27214) [M::mem_pestat] mean and std.dev: (2985.76, 3644.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 36116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 321, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3607) [M::mem_pestat] mean and std.dev: (805.53, 979.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698710 sequences (100000156 bp) in 2.585 CPU sec, 2.686 real sec... [M::process] read 696962 sequences (100000114 bp) in 1.980 CPU sec, 1.976 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 285663, 29, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 353, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (333.96, 230.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.01, 135.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (115, 427, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3157) [M::mem_pestat] mean and std.dev: (661.48, 681.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4171) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 338, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1471) [M::mem_pestat] mean and std.dev: (372.00, 216.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697668 reads in 182.061 CPU sec, 5.891 real sec [M::mem_process_seqs] Processed 697570 reads in 114.864 CPU sec, 3.745 real sec sorting block 192, #reads = 1076199 mem = 536880066 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 286421, 16, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 290, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2997) [M::mem_pestat] mean and std.dev: (609.29, 649.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (265.52, 132.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 240, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4063) [M::mem_pestat] mean and std.dev: (318.46, 380.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 476, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1675) [M::mem_pestat] mean and std.dev: (491.74, 322.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696400 sequences (100000072 bp) in 2.507 CPU sec, 2.581 real sec... [M::process] read 696118 sequences (100000057 bp) in 1.960 CPU sec, 1.960 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 285188, 26, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 220, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (328.73, 297.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.96, 135.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 405, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4376) [M::mem_pestat] mean and std.dev: (936.81, 1009.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 258, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2907) [M::mem_pestat] mean and std.dev: (603.33, 696.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3812) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698710 reads in 178.563 CPU sec, 5.774 real sec [M::mem_process_seqs] Processed 696962 reads in 112.807 CPU sec, 3.578 real sec sorting block 193, #reads = 1077456 mem = 536879828 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285262, 19, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 239, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (294.22, 191.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.80, 136.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (821, 827, 2766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6656) [M::mem_pestat] mean and std.dev: (1429.35, 1301.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 204, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (244.40, 147.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696086 sequences (100000100 bp) in 2.660 CPU sec, 2.768 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 285506, 22, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 230, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (291.93, 203.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (274.88, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (468, 721, 1937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4875) [M::mem_pestat] mean and std.dev: (969.06, 926.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 347, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2253) [M::mem_pestat] mean and std.dev: (358.02, 246.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695576 sequences (100000201 bp) in 1.950 CPU sec, 1.953 real sec... [M::mem_process_seqs] Processed 696400 reads in 166.785 CPU sec, 5.306 real sec sorting block 194, #reads = 1077750 mem = 536877587 peak = 1149726720 [M::mem_process_seqs] Processed 696118 reads in 109.944 CPU sec, 3.739 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285575, 15, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 258, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (229.67, 93.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.47, 136.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (464, 703, 1682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4118) [M::mem_pestat] mean and std.dev: (1017.73, 848.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 225, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416) [M::mem_pestat] mean and std.dev: (309.17, 226.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695628 sequences (100000173 bp) in 2.575 CPU sec, 2.677 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285443, 23, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 294, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1805) [M::mem_pestat] mean and std.dev: (397.82, 391.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (274.72, 136.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (365, 947, 2733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7469) [M::mem_pestat] mean and std.dev: (1133.65, 1332.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 279, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133) [M::mem_pestat] mean and std.dev: (348.82, 233.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695346 sequences (100000199 bp) in 1.940 CPU sec, 1.940 real sec... [M::mem_process_seqs] Processed 696086 reads in 167.678 CPU sec, 5.575 real sec sorting block 195, #reads = 1078330 mem = 536879163 peak = 1149726720 [M::mem_process_seqs] Processed 695576 reads in 109.313 CPU sec, 3.538 real sec sorting block 196, #reads = 1077203 mem = 536877847 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 285218, 10, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 261, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (246.24, 131.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.56, 136.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 1349, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3759) [M::mem_pestat] mean and std.dev: (928.56, 636.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4964) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 351, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (318.32, 188.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694880 sequences (100000277 bp) in 2.843 CPU sec, 3.000 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284913, 12, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 383, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3693) [M::mem_pestat] mean and std.dev: (678.11, 615.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 249, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (273.28, 134.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 1030, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3104) [M::mem_pestat] mean and std.dev: (773.27, 476.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4015) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1733) [M::mem_pestat] mean and std.dev: (356.42, 304.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695628 reads in 161.357 CPU sec, 5.199 real sec [M::process] read 695238 sequences (100000221 bp) in 2.087 CPU sec, 2.112 real sec... [M::mem_process_seqs] Processed 695346 reads in 113.105 CPU sec, 3.589 real sec sorting block 197, #reads = 1077383 mem = 536876128 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284642, 12, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (257.19, 142.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (274.64, 135.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 698, 3018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8628) [M::mem_pestat] mean and std.dev: (1838.58, 2377.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 267, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (275.55, 107.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695038 sequences (100000297 bp) in 2.697 CPU sec, 2.851 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285280, 18, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 267, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (334.30, 240.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 250, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (275.94, 137.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (727, 1427, 3177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8077) [M::mem_pestat] mean and std.dev: (2058.83, 2167.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 271, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (265.67, 160.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694880 reads in 157.899 CPU sec, 5.024 real sec [M::mem_process_seqs] Processed 695238 reads in 106.161 CPU sec, 3.349 real sec [M::process] read 694416 sequences (100000040 bp) in 2.247 CPU sec, 2.287 real sec... sorting block 198, #reads = 1076796 mem = 536878224 peak = 1149726720 [M::process] read 694880 sequences (100000071 bp) in 2.337 CPU sec, 2.415 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 284636, 22, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 264, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2437) [M::mem_pestat] mean and std.dev: (493.20, 514.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 252, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (276.01, 135.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (255, 669, 1630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4380) [M::mem_pestat] mean and std.dev: (1027.76, 1071.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5755) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 256, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1800) [M::mem_pestat] mean and std.dev: (397.38, 338.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694238 sequences (100000301 bp) in 1.850 CPU sec, 1.844 real sec... [M::mem_process_seqs] Processed 694416 reads in 86.657 CPU sec, 2.725 real sec sorting block 199, #reads = 1078036 mem = 536881293 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284840, 12, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 175, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (187.69, 92.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 250, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (274.88, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 369, 1710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4746) [M::mem_pestat] mean and std.dev: (905.25, 1054.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 349, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2491) [M::mem_pestat] mean and std.dev: (561.77, 444.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 285162, 32, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 342, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (342.40, 191.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (274.98, 135.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (410, 771, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2135) [M::mem_pestat] mean and std.dev: (712.37, 465.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 390, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1695) [M::mem_pestat] mean and std.dev: (412.57, 288.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695038 reads in 241.212 CPU sec, 7.654 real sec [M::process] read 693676 sequences (100000243 bp) in 2.462 CPU sec, 2.501 real sec... [M::mem_process_seqs] Processed 694880 reads in 123.040 CPU sec, 4.025 real sec sorting block 200, #reads = 1078722 mem = 536881275 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284715, 21, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 420, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1308) [M::mem_pestat] mean and std.dev: (367.87, 183.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 253, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (277.79, 137.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 861, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2621) [M::mem_pestat] mean and std.dev: (958.84, 452.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 271, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (422.48, 390.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694084 sequences (100000129 bp) in 2.500 CPU sec, 2.658 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284130, 17, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 378, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2987) [M::mem_pestat] mean and std.dev: (593.19, 616.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (278.14, 137.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (655, 743, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2272) [M::mem_pestat] mean and std.dev: (728.88, 398.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 300, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4585) [M::mem_pestat] mean and std.dev: (776.35, 934.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694238 reads in 153.678 CPU sec, 5.005 real sec sorting block 201, #reads = 1077856 mem = 536878648 peak = 1149726720 [M::process] read 694024 sequences (100000056 bp) in 2.109 CPU sec, 2.153 real sec... [M::mem_process_seqs] Processed 693676 reads in 104.462 CPU sec, 3.416 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 284081, 15, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 359, 757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1845) [M::mem_pestat] mean and std.dev: (422.45, 340.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (278.53, 137.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (78, 226, 2661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7827) [M::mem_pestat] mean and std.dev: (749.31, 1038.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2010) [M::mem_pestat] mean and std.dev: (332.43, 318.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 202, #reads = 1077707 mem = 536879133 peak = 1149726720 [M::process] read 693928 sequences (100000130 bp) in 2.473 CPU sec, 2.599 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285191, 8, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 335, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338) [M::mem_pestat] mean and std.dev: (353.25, 194.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (279.04, 138.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 350, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1553) [M::mem_pestat] mean and std.dev: (457.72, 345.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694084 reads in 153.244 CPU sec, 4.983 real sec [M::process] read 693358 sequences (100000102 bp) in 1.895 CPU sec, 1.900 real sec... [M::mem_process_seqs] Processed 694024 reads in 101.932 CPU sec, 3.225 real sec sorting block 203, #reads = 1078532 mem = 536882763 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284879, 14, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 327, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2388) [M::mem_pestat] mean and std.dev: (478.10, 416.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (278.90, 138.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (405, 835, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2979) [M::mem_pestat] mean and std.dev: (904.36, 647.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 316, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4511) [M::mem_pestat] mean and std.dev: (824.61, 917.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693280 sequences (100000081 bp) in 2.447 CPU sec, 2.545 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 284840, 17, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 374, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3058) [M::mem_pestat] mean and std.dev: (692.09, 707.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (279.93, 138.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 482, 883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2347) [M::mem_pestat] mean and std.dev: (453.38, 351.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3079) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 377, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171) [M::mem_pestat] mean and std.dev: (344.13, 209.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693928 reads in 151.392 CPU sec, 4.817 real sec [M::mem_process_seqs] Processed 693358 reads in 101.699 CPU sec, 3.210 real sec [M::process] read 693342 sequences (100000262 bp) in 2.121 CPU sec, 2.155 real sec... sorting block 204, #reads = 1079412 mem = 536880339 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284248, 11, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 298, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2404) [M::mem_pestat] mean and std.dev: (555.04, 530.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (280.23, 138.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 444, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3551) [M::mem_pestat] mean and std.dev: (687.45, 529.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 362, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1413) [M::mem_pestat] mean and std.dev: (357.44, 219.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693610 sequences (99870656 bp) in 2.607 CPU sec, 2.700 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 284788, 21, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 299, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (295.39, 129.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (281.21, 139.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 944, 2039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5793) [M::mem_pestat] mean and std.dev: (1262.38, 1076.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 263, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (266.63, 160.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693280 reads in 153.185 CPU sec, 4.954 real sec [M::mem_process_seqs] Processed 693342 reads in 100.072 CPU sec, 3.215 real sec sorting block 205, #reads = 1079644 mem = 536879021 peak = 1149726720 sorting block 206, #reads = 1079047 mem = 536881011 peak = 1149726720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284352, 27, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 332, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (287.66, 128.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (277.62, 138.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 461, 1615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4097) [M::mem_pestat] mean and std.dev: (873.50, 910.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 371, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1680) [M::mem_pestat] mean and std.dev: (456.35, 348.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693610 reads in 89.924 CPU sec, 2.991 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 222991538 overall: 70154358784 mem 33537.247 user 771.797 sys 1131.421 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_5TB-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:1136_5TB-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_5TB-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_5TB-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_5TB-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 1131.455 sec; CPU: 34309.047 sec sorting block 207, #reads = 709394 mem = 354342214 peak = 1149726720 uniq header count 1 merging 208 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 12217816905 output reads: 224450074 bam_mem_sort: 208 calls 123.533 user 2.217 sys 129.325 real bam_write: 208 calls 105.793 user 7.297 sys 216.232 real execute: 1 calls 47.464 user 3.014 sys 1214.512 real merge_files: 1 calls 47.423 user 2.987 sys 82.725 real parse_chunk: 3323 calls 182.976 user 47.238 sys 281.432 real read_chunk: 8979 calls 110.037 user 54.035 sys 1096.696 real sort_block: 1 calls 75.060 user 53.013 sys 1053.856 real write_chunk: 5654 calls 47.045 user 2.513 sys 81.529 real overall: 3642544128 mem 3341.155 user 141.490 sys 1214.535 real ls: cannot access '*.cram': No such file or directory