File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7a/7ff863e6f3e9243abe4c0b90a84524/.command.log
Size
195.5 KB
Task
DAQ:SENTIEON_BWAMEM (659_cAd-T1-TDNA-1_A23MHWWLT4_1;L004;0001)
Attempt

Content

View in new tab 
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7a/7ff863e6f3e9243abe4c0b90a84524/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/dc/c8056f8ef47537e7b5d0d51d714244/659_cAd-T1-TDNA-1_A23MHWWLT4_1/659_cAd-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7a/7ff863e6f3e9243abe4c0b90a84524/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/dc/c8056f8ef47537e7b5d0d51d714244/659_cAd-T1-TDNA-1_A23MHWWLT4_1/659_cAd-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cAd-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1516.883 CPU sec, 53.718 real sec
[M::process] read 685226 sequences (100000164 bp) in 1.053 CPU sec, 1.056 real sec...
[M::process] read 684572 sequences (100000130 bp) in 1.554 CPU sec, 1.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287714, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (305.82, 141.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684632 sequences (100000222 bp) in 1.555 CPU sec, 1.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287818, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (307.23, 141.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684276 sequences (100000155 bp) in 1.449 CPU sec, 1.451 real sec...
[M::mem_process_seqs] Processed 685226 reads in 159.236 CPU sec, 5.046 real sec
[M::mem_process_seqs] Processed 684572 reads in 95.598 CPU sec, 3.029 real sec
sorting block 0, #reads = 1087954 mem = 536878978 peak = 591130624
[M::process] read 683802 sequences (100000238 bp) in 1.979 CPU sec, 2.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287959, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 292, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (307.42, 141.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683298 sequences (100000093 bp) in 1.590 CPU sec, 1.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 288146, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 124, 124)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 124)
[M::mem_pestat] mean and std.dev: (124.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (124, 124)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (309.72, 142.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 684632 reads in 149.522 CPU sec, 4.738 real sec
[M::mem_process_seqs] Processed 684276 reads in 96.708 CPU sec, 3.088 real sec
sorting block 1, #reads = 1087123 mem = 536879111 peak = 694628352
[M::process] read 683176 sequences (100000012 bp) in 1.752 CPU sec, 1.793 real sec...
[M::process] read 682678 sequences (100000262 bp) in 1.600 CPU sec, 1.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287479, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (313.83, 144.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (325, 367, 813)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789)
[M::mem_pestat] mean and std.dev: (597.40, 456.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2423)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683298 reads in 84.329 CPU sec, 2.691 real sec
sorting block 2, #reads = 1086496 mem = 536879797 peak = 838488064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287227, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (311.58, 143.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287363, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 346, 686)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1680)
[M::mem_pestat] mean and std.dev: (347.22, 196.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2177)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (315.95, 145.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682586 sequences (100000278 bp) in 1.672 CPU sec, 1.692 real sec...
[M::mem_process_seqs] Processed 683802 reads in 224.147 CPU sec, 7.125 real sec
[M::mem_process_seqs] Processed 683176 reads in 174.920 CPU sec, 5.588 real sec
sorting block 3, #reads = 1085546 mem = 536879385 peak = 838488064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287326, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (319.79, 147.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681990 sequences (100000003 bp) in 2.606 CPU sec, 2.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286968, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (320.31, 148.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681978 sequences (100000212 bp) in 1.625 CPU sec, 1.632 real sec...
[M::mem_process_seqs] Processed 682678 reads in 147.319 CPU sec, 4.689 real sec
[M::mem_process_seqs] Processed 682586 reads in 98.762 CPU sec, 3.158 real sec
sorting block 4, #reads = 1085621 mem = 536878652 peak = 838488064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287158, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (322.50, 148.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681770 sequences (100000260 bp) in 2.058 CPU sec, 2.110 real sec...
[M::process] read 681622 sequences (100000208 bp) in 1.463 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287065, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (322.51, 147.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (372, 466, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (184, 654)
[M::mem_pestat] mean and std.dev: (399.00, 97.02)
[M::mem_pestat] low and high boundaries for proper pairs: (11, 787)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681990 reads in 140.610 CPU sec, 4.482 real sec
[M::mem_process_seqs] Processed 681978 reads in 88.390 CPU sec, 2.830 real sec
sorting block 5, #reads = 1085683 mem = 536882036 peak = 838488064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286952, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (322.18, 148.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 7666, 7666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22666)
[M::mem_pestat] mean and std.dev: (4677.50, 3660.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 30166)
[M::mem_pestat] skip orientation RR
[M::process] read 681856 sequences (100000290 bp) in 2.147 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287441, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (321.13, 147.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681942 sequences (100000261 bp) in 1.739 CPU sec, 1.748 real sec...
[M::mem_process_seqs] Processed 681770 reads in 144.097 CPU sec, 4.600 real sec
sorting block 6, #reads = 1087079 mem = 536878766 peak = 838488064
[M::mem_process_seqs] Processed 681622 reads in 99.609 CPU sec, 3.208 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286907, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (320.50, 147.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1085936 mem = 536878430 peak = 904962048
[M::process] read 681802 sequences (100000204 bp) in 3.419 CPU sec, 3.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286627, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (319.19, 145.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681856 reads in 181.371 CPU sec, 5.981 real sec
[M::mem_process_seqs] Processed 681942 reads in 126.167 CPU sec, 4.116 real sec
[M::process] read 682034 sequences (100000214 bp) in 2.674 CPU sec, 2.785 real sec...
sorting block 8, #reads = 1085076 mem = 536878659 peak = 904962048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286768, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 125, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (146.78, 50.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 534)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (319.10, 146.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682060 sequences (100000240 bp) in 3.473 CPU sec, 3.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286925, 11, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 302, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (318.22, 145.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 112, 159)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 253)
[M::mem_pestat] mean and std.dev: (111.33, 39.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 300)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 681802 reads in 200.249 CPU sec, 6.692 real sec
[M::mem_process_seqs] Processed 682034 reads in 133.122 CPU sec, 4.398 real sec
[M::process] read 681798 sequences (100000025 bp) in 2.475 CPU sec, 2.551 real sec...
sorting block 9, #reads = 1085620 mem = 536878937 peak = 904962048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286863, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 302, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (318.74, 146.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681646 sequences (100000055 bp) in 3.531 CPU sec, 3.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287192, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (320.48, 147.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682060 reads in 189.723 CPU sec, 6.200 real sec
[M::mem_process_seqs] Processed 681798 reads in 120.310 CPU sec, 3.872 real sec
sorting block 10, #reads = 1085365 mem = 536882296 peak = 904962048
[M::process] read 681188 sequences (100000250 bp) in 2.985 CPU sec, 3.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286853, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (319.97, 146.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681382 sequences (100000299 bp) in 2.927 CPU sec, 3.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285927, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (321.95, 147.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681646 reads in 180.138 CPU sec, 5.887 real sec
sorting block 11, #reads = 1086609 mem = 536882294 peak = 904962048
[M::mem_process_seqs] Processed 681188 reads in 123.745 CPU sec, 4.020 real sec
[M::process] read 680978 sequences (100000135 bp) in 2.792 CPU sec, 3.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286096, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (320.42, 145.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 12, #reads = 1085194 mem = 536878473 peak = 904962048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286197, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (323.03, 146.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680780 sequences (100000194 bp) in 2.773 CPU sec, 3.199 real sec...
[M::mem_process_seqs] Processed 681382 reads in 179.234 CPU sec, 5.942 real sec
[M::mem_process_seqs] Processed 680978 reads in 121.959 CPU sec, 3.999 real sec
[M::process] read 680706 sequences (100000086 bp) in 2.762 CPU sec, 3.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286133, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (323.18, 145.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1084591 mem = 536886620 peak = 904962048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286900, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (323.02, 145.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680264 sequences (100000167 bp) in 2.677 CPU sec, 3.021 real sec...
[M::mem_process_seqs] Processed 680780 reads in 176.545 CPU sec, 5.968 real sec
[M::mem_process_seqs] Processed 680706 reads in 119.650 CPU sec, 4.151 real sec
sorting block 14, #reads = 1085741 mem = 536879738 peak = 904962048
[M::process] read 680112 sequences (100000105 bp) in 2.712 CPU sec, 3.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286289, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (325.59, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286666, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (327.59, 148.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680070 sequences (100000068 bp) in 2.654 CPU sec, 3.099 real sec...
[M::mem_process_seqs] Processed 680264 reads in 174.050 CPU sec, 6.039 real sec
[M::mem_process_seqs] Processed 680112 reads in 116.598 CPU sec, 4.200 real sec
sorting block 15, #reads = 1084576 mem = 536880198 peak = 904962048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286452, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (328.87, 148.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679558 sequences (100000234 bp) in 2.938 CPU sec, 3.298 real sec...
[M::mem_process_seqs] Processed 680070 reads in 100.078 CPU sec, 3.477 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285834, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 16, #reads = 1084989 mem = 536877840 peak = 904962048
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (330.19, 148.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682596 sequences (100000221 bp) in 2.861 CPU sec, 3.254 real sec...
[M::mem_process_seqs] Processed 679558 reads in 99.900 CPU sec, 3.597 real sec
[M::process] read 685298 sequences (100000041 bp) in 3.181 CPU sec, 3.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287140, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (317.99, 145.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1084915 mem = 536879938 peak = 904962048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287457, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (308.84, 145.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684568 sequences (100000260 bp) in 2.577 CPU sec, 2.828 real sec...
[M::mem_process_seqs] Processed 682596 reads in 179.310 CPU sec, 6.039 real sec
[M::mem_process_seqs] Processed 685298 reads in 119.941 CPU sec, 4.431 real sec
[M::process] read 684458 sequences (100000185 bp) in 2.523 CPU sec, 2.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287750, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 296, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (312.29, 145.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 18, #reads = 1085209 mem = 536877806 peak = 904962048
[M::process] read 684156 sequences (100000184 bp) in 2.399 CPU sec, 2.653 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286840, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 296, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (311.34, 144.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684568 reads in 174.488 CPU sec, 5.680 real sec
[M::mem_process_seqs] Processed 684458 reads in 116.754 CPU sec, 3.748 real sec
sorting block 19, #reads = 1089109 mem = 536878204 peak = 904962048
[M::process] read 683906 sequences (100000112 bp) in 2.240 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287852, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (313.45, 146.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683316 sequences (100000017 bp) in 1.991 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287041, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (313.94, 145.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683080 sequences (100000205 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 684156 reads in 142.361 CPU sec, 4.514 real sec
sorting block 20, #reads = 1088284 mem = 536879288 peak = 904962048
[M::mem_process_seqs] Processed 683906 reads in 91.463 CPU sec, 2.885 real sec
sorting block 21, #reads = 1087589 mem = 536879774 peak = 904962048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287152, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (270, 344, 785)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815)
[M::mem_pestat] mean and std.dev: (449.90, 221.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2330)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (317.17, 146.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682632 sequences (100000027 bp) in 2.264 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287335, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (318.62, 147.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682380 sequences (100000114 bp) in 1.490 CPU sec, 1.489 real sec...
[M::mem_process_seqs] Processed 683316 reads in 137.986 CPU sec, 4.365 real sec
[M::mem_process_seqs] Processed 683080 reads in 86.628 CPU sec, 2.738 real sec
sorting block 22, #reads = 1087560 mem = 536875925 peak = 904962048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287098, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 306, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (320.94, 148.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (295, 529, 1186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2968)
[M::mem_pestat] mean and std.dev: (660.33, 562.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3859)
[M::mem_pestat] skip orientation RR
[M::process] read 682312 sequences (100000042 bp) in 2.341 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287263, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (322.96, 149.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682096 sequences (100000092 bp) in 1.640 CPU sec, 1.638 real sec...
[M::mem_process_seqs] Processed 682632 reads in 140.185 CPU sec, 4.425 real sec
[M::mem_process_seqs] Processed 682380 reads in 91.880 CPU sec, 2.903 real sec
sorting block 23, #reads = 1087082 mem = 536878123 peak = 904962048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286828, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 309, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (323.72, 149.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682034 sequences (100000017 bp) in 2.243 CPU sec, 2.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286731, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682130 sequences (100000183 bp) in 1.560 CPU sec, 1.568 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (324.10, 150.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682312 reads in 142.176 CPU sec, 4.499 real sec
[M::mem_process_seqs] Processed 682096 reads in 92.068 CPU sec, 2.911 real sec
sorting block 24, #reads = 1086558 mem = 536881849 peak = 904962048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287354, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 309, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (324.58, 150.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682060 sequences (100000171 bp) in 2.600 CPU sec, 2.652 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286945, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (322.60, 149.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682034 reads in 141.128 CPU sec, 4.464 real sec
[M::process] read 682024 sequences (100000220 bp) in 1.745 CPU sec, 1.748 real sec...
sorting block 25, #reads = 1086163 mem = 536878136 peak = 904962048
[M::mem_process_seqs] Processed 682130 reads in 91.242 CPU sec, 2.864 real sec
sorting block 26, #reads = 1086696 mem = 536877756 peak = 919982080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287501, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (323.02, 149.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682224 sequences (100000006 bp) in 2.152 CPU sec, 2.202 real sec...
[M::process] read 682416 sequences (100000213 bp) in 1.550 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286811, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (320.87, 148.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682060 reads in 144.440 CPU sec, 4.575 real sec
[M::mem_process_seqs] Processed 682024 reads in 96.191 CPU sec, 3.051 real sec
sorting block 27, #reads = 1086413 mem = 536880039 peak = 919982080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287331, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (317.71, 145.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682476 sequences (100000092 bp) in 2.928 CPU sec, 2.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286653, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 300, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (315.76, 144.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682224 reads in 167.336 CPU sec, 5.280 real sec
[M::process] read 682058 sequences (100000068 bp) in 2.246 CPU sec, 2.256 real sec...
[M::mem_process_seqs] Processed 682416 reads in 110.356 CPU sec, 3.468 real sec
sorting block 28, #reads = 1085697 mem = 536877650 peak = 919982080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286484, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (316.35, 145.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681592 sequences (100000004 bp) in 2.860 CPU sec, 2.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286937, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (317.74, 145.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682476 reads in 169.875 CPU sec, 5.363 real sec
[M::process] read 681624 sequences (100000191 bp) in 2.188 CPU sec, 2.196 real sec...
sorting block 29, #reads = 1085948 mem = 536882927 peak = 919982080
[M::mem_process_seqs] Processed 682058 reads in 111.278 CPU sec, 3.501 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286776, 0, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (318.98, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 310, 1591)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4389)
[M::mem_pestat] mean and std.dev: (644.22, 665.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5788)
[M::mem_pestat] skip orientation RR
[M::process] read 681716 sequences (100000036 bp) in 2.872 CPU sec, 2.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286566, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (308, 317, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (400.70, 212.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 681218 sequences (100000140 bp) in 1.770 CPU sec, 1.770 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (318.68, 145.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 681592 reads in 170.044 CPU sec, 5.375 real sec
sorting block 30, #reads = 1085449 mem = 536879759 peak = 919982080
[M::mem_process_seqs] Processed 681624 reads in 110.233 CPU sec, 3.489 real sec
sorting block 31, #reads = 1085634 mem = 536880345 peak = 972701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287363, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (319.26, 145.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681056 sequences (100000027 bp) in 2.465 CPU sec, 2.533 real sec...
[M::process] read 680766 sequences (100000112 bp) in 1.620 CPU sec, 1.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286871, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (321.55, 146.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681716 reads in 165.217 CPU sec, 5.234 real sec
[M::mem_process_seqs] Processed 681218 reads in 109.215 CPU sec, 3.474 real sec
sorting block 32, #reads = 1085607 mem = 536882534 peak = 972701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286954, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (323.77, 147.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681042 sequences (100000150 bp) in 2.983 CPU sec, 3.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286751, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 309, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (323.63, 147.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681056 reads in 164.158 CPU sec, 5.169 real sec
[M::process] read 680748 sequences (100000262 bp) in 2.445 CPU sec, 2.449 real sec...
[M::mem_process_seqs] Processed 680766 reads in 107.683 CPU sec, 3.378 real sec
sorting block 33, #reads = 1086077 mem = 536883771 peak = 972701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287124, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (324.93, 149.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681158 sequences (100000202 bp) in 2.734 CPU sec, 2.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286337, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 232, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (232, 232)
[M::mem_pestat] mean and std.dev: (232.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (232, 232)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (325.18, 148.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681338 sequences (100000292 bp) in 1.750 CPU sec, 1.743 real sec...
[M::mem_process_seqs] Processed 681042 reads in 166.665 CPU sec, 5.275 real sec
sorting block 34, #reads = 1085597 mem = 536884440 peak = 972701696
[M::mem_process_seqs] Processed 680748 reads in 108.595 CPU sec, 3.429 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286782, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 309, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (325.42, 150.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686458 sequences (100000101 bp) in 3.002 CPU sec, 3.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286521, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 311, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (326.81, 150.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681158 reads in 171.650 CPU sec, 5.414 real sec
sorting block 35, #reads = 1085297 mem = 536879491 peak = 972701696
[M::process] read 685416 sequences (100000153 bp) in 2.435 CPU sec, 2.442 real sec...
[M::mem_process_seqs] Processed 681338 reads in 114.012 CPU sec, 3.579 real sec
sorting block 36, #reads = 1083580 mem = 536878195 peak = 1019658240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288024, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (307, 394, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 568)
[M::mem_pestat] mean and std.dev: (369.50, 64.55)
[M::mem_pestat] low and high boundaries for proper pairs: (46, 655)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (304.25, 142.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685604 sequences (100000066 bp) in 2.887 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287374, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (307.63, 142.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686458 reads in 165.701 CPU sec, 5.232 real sec
[M::process] read 685170 sequences (100000109 bp) in 2.253 CPU sec, 2.258 real sec...
[M::mem_process_seqs] Processed 685416 reads in 104.078 CPU sec, 3.265 real sec
sorting block 37, #reads = 1087923 mem = 536876281 peak = 1019658240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288410, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.36, 141.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684016 sequences (100000278 bp) in 2.886 CPU sec, 2.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288240, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 295, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (309.19, 142.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685604 reads in 174.980 CPU sec, 5.530 real sec
[M::process] read 683936 sequences (100000181 bp) in 2.299 CPU sec, 2.300 real sec...
[M::mem_process_seqs] Processed 685170 reads in 114.622 CPU sec, 3.609 real sec
sorting block 38, #reads = 1087849 mem = 536880210 peak = 1019658240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286847, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 297, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (311.13, 142.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 157, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (144.22, 53.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 535)
[M::mem_pestat] skip orientation RR
[M::process] read 683272 sequences (100000011 bp) in 2.847 CPU sec, 2.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287390, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (312.58, 142.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684016 reads in 172.444 CPU sec, 5.449 real sec
[M::process] read 682962 sequences (100000273 bp) in 2.359 CPU sec, 2.377 real sec...
sorting block 39, #reads = 1086972 mem = 536879808 peak = 1019658240
[M::mem_process_seqs] Processed 683936 reads in 112.579 CPU sec, 3.534 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287460, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 303, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (315.57, 143.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682582 sequences (100000275 bp) in 2.500 CPU sec, 2.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287096, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (316.44, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682278 sequences (100000270 bp) in 1.890 CPU sec, 1.883 real sec...
[M::mem_process_seqs] Processed 683272 reads in 165.933 CPU sec, 5.263 real sec
sorting block 40, #reads = 1086454 mem = 536879625 peak = 1019658240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287158, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (316.95, 143.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682204 sequences (100000131 bp) in 2.650 CPU sec, 2.656 real sec...
[M::mem_process_seqs] Processed 682582 reads in 97.019 CPU sec, 3.050 real sec
sorting block 41, #reads = 1085866 mem = 536878280 peak = 1065394176
[M::mem_process_seqs] Processed 682962 reads in 224.639 CPU sec, 7.112 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287303, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (319.55, 144.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682250 sequences (100000058 bp) in 2.987 CPU sec, 3.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287263, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 306, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (319.29, 144.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682278 reads in 188.965 CPU sec, 5.959 real sec
sorting block 42, #reads = 1086851 mem = 536878123 peak = 1065394176
[M::mem_process_seqs] Processed 682204 reads in 114.096 CPU sec, 3.586 real sec
[M::process] read 681964 sequences (100000144 bp) in 2.554 CPU sec, 2.592 real sec...
sorting block 43, #reads = 1086989 mem = 536880007 peak = 1065394176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286558, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (319.61, 145.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681850 sequences (100000270 bp) in 2.528 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286952, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (321.64, 146.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682250 reads in 174.707 CPU sec, 5.527 real sec
[M::process] read 682220 sequences (100000012 bp) in 2.230 CPU sec, 2.224 real sec...
[M::mem_process_seqs] Processed 681964 reads in 114.598 CPU sec, 3.615 real sec
sorting block 44, #reads = 1086457 mem = 536880323 peak = 1065394176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286581, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (321.56, 146.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682074 sequences (100000003 bp) in 3.074 CPU sec, 3.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287548, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (317.19, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681850 reads in 171.612 CPU sec, 5.413 real sec
[M::process] read 681924 sequences (100000270 bp) in 2.304 CPU sec, 2.311 real sec...
[M::mem_process_seqs] Processed 682220 reads in 112.918 CPU sec, 3.550 real sec
sorting block 45, #reads = 1086144 mem = 536879560 peak = 1065394176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286978, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (318.26, 145.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681822 sequences (100000185 bp) in 2.783 CPU sec, 2.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286751, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (318.56, 145.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681780 sequences (100000256 bp) in 2.300 CPU sec, 2.295 real sec...
[M::mem_process_seqs] Processed 682074 reads in 172.080 CPU sec, 5.430 real sec
sorting block 46, #reads = 1086458 mem = 536883301 peak = 1065394176
[M::mem_process_seqs] Processed 681924 reads in 111.979 CPU sec, 3.524 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286903, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (317.36, 144.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681774 sequences (100000072 bp) in 3.325 CPU sec, 3.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287386, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (318.85, 145.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 411, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (394.80, 246.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1380)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681822 reads in 175.075 CPU sec, 5.526 real sec
sorting block 47, #reads = 1084747 mem = 536879068 peak = 1065394176
[M::process] read 682050 sequences (100000293 bp) in 2.469 CPU sec, 2.477 real sec...
[M::mem_process_seqs] Processed 681780 reads in 114.742 CPU sec, 3.613 real sec
sorting block 48, #reads = 1085116 mem = 536879742 peak = 1065394176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287066, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (319.58, 146.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681644 sequences (100000200 bp) in 2.828 CPU sec, 2.881 real sec...
[M::process] read 681562 sequences (100000079 bp) in 1.720 CPU sec, 1.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287263, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (318.17, 145.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681774 reads in 177.392 CPU sec, 5.620 real sec
[M::mem_process_seqs] Processed 682050 reads in 116.726 CPU sec, 3.692 real sec
sorting block 49, #reads = 1086022 mem = 536880780 peak = 1065394176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286688, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 303, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (317.26, 143.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681560 sequences (100000140 bp) in 2.701 CPU sec, 2.783 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287015, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (338, 338, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 407)
[M::mem_pestat] mean and std.dev: (344.57, 10.39)
[M::mem_pestat] low and high boundaries for proper pairs: (269, 430)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 306, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (319.50, 145.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681446 sequences (100000295 bp) in 2.170 CPU sec, 2.171 real sec...
[M::mem_process_seqs] Processed 681644 reads in 174.249 CPU sec, 5.500 real sec
[M::mem_process_seqs] Processed 681562 reads in 110.798 CPU sec, 3.508 real sec
sorting block 50, #reads = 1084523 mem = 536879309 peak = 1065394176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286653, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (321.24, 146.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681126 sequences (100000248 bp) in 2.511 CPU sec, 2.580 real sec...
[M::process] read 681508 sequences (100000000 bp) in 1.800 CPU sec, 1.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287016, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 306, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (322.01, 147.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681560 reads in 177.114 CPU sec, 5.615 real sec
sorting block 51, #reads = 1083974 mem = 536880170 peak = 1065394176
[M::mem_process_seqs] Processed 681446 reads in 116.495 CPU sec, 3.697 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286794, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (322.37, 147.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681296 sequences (100000060 bp) in 2.974 CPU sec, 3.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286968, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (322.25, 148.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681126 reads in 170.234 CPU sec, 5.374 real sec
sorting block 52, #reads = 1083173 mem = 536876838 peak = 1065394176
[M::process] read 682408 sequences (100000066 bp) in 2.432 CPU sec, 2.436 real sec...
[M::mem_process_seqs] Processed 681508 reads in 110.043 CPU sec, 3.453 real sec
sorting block 53, #reads = 1084775 mem = 536878268 peak = 1070403584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286414, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (322.80, 148.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1792, 1792, 4129)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8803)
[M::mem_pestat] mean and std.dev: (1953.77, 1360.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11140)
[M::mem_pestat] skip orientation RR
[M::process] read 685454 sequences (100000252 bp) in 2.878 CPU sec, 2.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286829, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (318.25, 146.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685102 sequences (100000122 bp) in 2.220 CPU sec, 2.221 real sec...
[M::mem_process_seqs] Processed 681296 reads in 180.016 CPU sec, 5.691 real sec
[M::mem_process_seqs] Processed 682408 reads in 121.657 CPU sec, 3.832 real sec
sorting block 54, #reads = 1083326 mem = 536877799 peak = 1070403584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287300, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (306.45, 142.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 418, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (341.82, 106.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RR
[M::process] read 684652 sequences (100000054 bp) in 2.936 CPU sec, 3.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287621, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 295, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (309.42, 143.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684574 sequences (100000116 bp) in 2.190 CPU sec, 2.184 real sec...
[M::mem_process_seqs] Processed 685454 reads in 172.969 CPU sec, 5.455 real sec
[M::mem_process_seqs] Processed 685102 reads in 113.716 CPU sec, 3.586 real sec
sorting block 55, #reads = 1084332 mem = 536878343 peak = 1070403584
[M::process] read 683808 sequences (100000154 bp) in 2.689 CPU sec, 2.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287739, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (311.83, 144.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683866 sequences (100000200 bp) in 1.830 CPU sec, 1.831 real sec...
[M::mem_process_seqs] Processed 684574 reads in 96.087 CPU sec, 3.018 real sec
sorting block 56, #reads = 1088327 mem = 536877059 peak = 1070403584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287443, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 296, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (310.82, 143.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287576, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (313.62, 143.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (758, 931, 2255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5249)
[M::mem_pestat] mean and std.dev: (1373.69, 1166.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6746)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684652 reads in 270.876 CPU sec, 8.579 real sec
[M::process] read 683430 sequences (100000013 bp) in 2.285 CPU sec, 2.299 real sec...
[M::mem_process_seqs] Processed 683808 reads in 135.995 CPU sec, 4.316 real sec
sorting block 57, #reads = 1088110 mem = 536876233 peak = 1070403584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287485, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 300, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (313.90, 144.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 358, 9646)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28516)
[M::mem_pestat] mean and std.dev: (2836.45, 4171.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 37951)
[M::mem_pestat] skip orientation RR
[M::process] read 683068 sequences (100000202 bp) in 2.542 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287306, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (315.94, 144.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682580 sequences (100000232 bp) in 1.820 CPU sec, 1.822 real sec...
[M::mem_process_seqs] Processed 683866 reads in 169.220 CPU sec, 5.367 real sec
sorting block 58, #reads = 1087122 mem = 536883063 peak = 1070403584
[M::mem_process_seqs] Processed 683430 reads in 109.545 CPU sec, 3.467 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287768, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (318.85, 146.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682692 sequences (100000093 bp) in 2.700 CPU sec, 2.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286247, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 306, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (319.29, 145.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683068 reads in 165.136 CPU sec, 5.227 real sec
sorting block 59, #reads = 1086709 mem = 536878207 peak = 1070403584
[M::process] read 682384 sequences (100000101 bp) in 2.446 CPU sec, 2.456 real sec...
[M::mem_process_seqs] Processed 682580 reads in 108.856 CPU sec, 3.418 real sec
sorting block 60, #reads = 1087338 mem = 536881619 peak = 1070403584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286826, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (318.36, 145.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682078 sequences (100000126 bp) in 2.997 CPU sec, 3.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286702, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (320.02, 145.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682692 reads in 170.379 CPU sec, 5.397 real sec
[M::process] read 682352 sequences (100000107 bp) in 2.257 CPU sec, 2.274 real sec...
[M::mem_process_seqs] Processed 682384 reads in 112.155 CPU sec, 3.522 real sec
sorting block 61, #reads = 1085189 mem = 536880305 peak = 1070403584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286905, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (319.11, 144.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682362 sequences (100000020 bp) in 2.699 CPU sec, 2.767 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286890, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (320.71, 146.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682078 reads in 171.793 CPU sec, 5.431 real sec
[M::process] read 683794 sequences (100000174 bp) in 2.419 CPU sec, 2.428 real sec...
[M::mem_process_seqs] Processed 682352 reads in 112.661 CPU sec, 3.539 real sec
sorting block 62, #reads = 1085802 mem = 536878686 peak = 1070403584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287175, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (321.69, 147.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683182 sequences (100000158 bp) in 2.515 CPU sec, 2.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287857, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (312.81, 145.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682586 sequences (100000062 bp) in 1.910 CPU sec, 1.909 real sec...
[M::mem_process_seqs] Processed 682362 reads in 173.724 CPU sec, 5.511 real sec
sorting block 63, #reads = 1085471 mem = 536878616 peak = 1070403584
[M::mem_process_seqs] Processed 683794 reads in 113.872 CPU sec, 3.603 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287352, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 296, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (311.95, 143.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682274 sequences (100000180 bp) in 2.903 CPU sec, 2.962 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287079, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 299, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (314.38, 143.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682224 sequences (100000161 bp) in 2.180 CPU sec, 2.185 real sec...
[M::mem_process_seqs] Processed 683182 reads in 173.834 CPU sec, 5.479 real sec
sorting block 64, #reads = 1086094 mem = 536882140 peak = 1070403584
[M::mem_process_seqs] Processed 682586 reads in 114.374 CPU sec, 3.606 real sec
sorting block 65, #reads = 1086229 mem = 536880312 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287531, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 300, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (314.63, 142.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681986 sequences (100000126 bp) in 2.465 CPU sec, 2.539 real sec...
[M::process] read 681886 sequences (100000201 bp) in 1.660 CPU sec, 1.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287073, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 299, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (313.26, 141.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682274 reads in 169.690 CPU sec, 5.380 real sec
[M::mem_process_seqs] Processed 682224 reads in 109.702 CPU sec, 3.482 real sec
sorting block 66, #reads = 1085302 mem = 536878501 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286513, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 300, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (314.34, 141.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 315, 2598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7478)
[M::mem_pestat] mean and std.dev: (1158.90, 1176.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9918)
[M::mem_pestat] skip orientation RR
[M::process] read 682028 sequences (100000065 bp) in 3.096 CPU sec, 3.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286409, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 299, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (313.84, 141.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681986 reads in 177.055 CPU sec, 5.580 real sec
[M::process] read 681900 sequences (100000162 bp) in 2.290 CPU sec, 2.283 real sec...
[M::mem_process_seqs] Processed 681886 reads in 118.106 CPU sec, 3.719 real sec
sorting block 67, #reads = 1085065 mem = 536877353 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286762, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 299, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (313.64, 141.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 247, 797)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1967)
[M::mem_pestat] mean and std.dev: (511.00, 498.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2552)
[M::mem_pestat] skip orientation RR
[M::process] read 681554 sequences (100000183 bp) in 2.670 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286713, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (480, 1000, 1310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2970)
[M::mem_pestat] mean and std.dev: (858.25, 476.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 299, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (312.44, 139.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681730 sequences (100000277 bp) in 2.210 CPU sec, 2.211 real sec...
[M::mem_process_seqs] Processed 682028 reads in 173.188 CPU sec, 5.469 real sec
sorting block 68, #reads = 1083988 mem = 536878382 peak = 1093652480
[M::mem_process_seqs] Processed 681900 reads in 114.340 CPU sec, 3.601 real sec
sorting block 69, #reads = 1084007 mem = 536877563 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286853, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 303, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (316.53, 142.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681472 sequences (100000211 bp) in 3.050 CPU sec, 3.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286637, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (318.31, 144.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681300 sequences (100000207 bp) in 2.260 CPU sec, 2.266 real sec...
[M::mem_process_seqs] Processed 681554 reads in 176.583 CPU sec, 5.569 real sec
[M::mem_process_seqs] Processed 681730 reads in 115.489 CPU sec, 3.641 real sec
sorting block 70, #reads = 1084519 mem = 536881195 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287039, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 623, 993)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2665)
[M::mem_pestat] mean and std.dev: (444.38, 288.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3501)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (319.86, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681160 sequences (100000126 bp) in 3.004 CPU sec, 3.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286724, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 305, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (320.61, 146.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683626 sequences (100000295 bp) in 1.750 CPU sec, 1.743 real sec...
[M::mem_process_seqs] Processed 681472 reads in 169.959 CPU sec, 5.371 real sec
[M::mem_process_seqs] Processed 681300 reads in 110.675 CPU sec, 3.503 real sec
sorting block 71, #reads = 1084421 mem = 536879112 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286344, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 569, 705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1875)
[M::mem_pestat] mean and std.dev: (397.40, 259.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (323.42, 147.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (985, 1863, 2814)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6472)
[M::mem_pestat] mean and std.dev: (1784.20, 814.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8301)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690182 sequences (100000092 bp) in 2.729 CPU sec, 2.784 real sec...
[M::process] read 688740 sequences (100000295 bp) in 1.780 CPU sec, 1.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287694, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (313.95, 146.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681160 reads in 175.420 CPU sec, 5.561 real sec
sorting block 72, #reads = 1083721 mem = 536881522 peak = 1093652480
[M::mem_process_seqs] Processed 683626 reads in 117.720 CPU sec, 3.724 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289730, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 264, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (287.71, 140.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686900 sequences (100000114 bp) in 2.963 CPU sec, 3.005 real sec...
[M::process] read 685898 sequences (100000059 bp) in 2.200 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288978, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 273, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (294.88, 142.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690182 reads in 193.771 CPU sec, 6.121 real sec
sorting block 73, #reads = 1083169 mem = 536877269 peak = 1093652480
[M::mem_process_seqs] Processed 688740 reads in 127.975 CPU sec, 4.056 real sec
sorting block 74, #reads = 1085915 mem = 536881959 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288749, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (303.20, 146.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685222 sequences (100000074 bp) in 2.943 CPU sec, 2.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 288619, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 468, 647)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625)
[M::mem_pestat] mean and std.dev: (366.33, 213.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2114)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (307.39, 147.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684886 sequences (100000096 bp) in 2.220 CPU sec, 2.217 real sec...
[M::mem_process_seqs] Processed 686900 reads in 182.956 CPU sec, 5.780 real sec
[M::mem_process_seqs] Processed 685898 reads in 118.952 CPU sec, 3.763 real sec
sorting block 75, #reads = 1087008 mem = 536879521 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288134, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (310.54, 148.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684346 sequences (100000031 bp) in 3.050 CPU sec, 3.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288236, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (312.21, 148.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684166 sequences (100000186 bp) in 2.340 CPU sec, 2.339 real sec...
[M::mem_process_seqs] Processed 685222 reads in 184.700 CPU sec, 5.831 real sec
[M::mem_process_seqs] Processed 684886 reads in 119.493 CPU sec, 3.772 real sec
sorting block 76, #reads = 1086841 mem = 536879751 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288201, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (313.92, 148.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683732 sequences (100000110 bp) in 2.839 CPU sec, 2.910 real sec...
[M::process] read 683408 sequences (100000094 bp) in 1.950 CPU sec, 1.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287739, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (770, 1212, 5802)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15866)
[M::mem_pestat] mean and std.dev: (2248.27, 2432.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20898)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (315.32, 148.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 684346 reads in 187.046 CPU sec, 5.924 real sec
sorting block 77, #reads = 1086228 mem = 536883846 peak = 1093652480
[M::mem_process_seqs] Processed 684166 reads in 120.579 CPU sec, 3.832 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287733, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (317.17, 149.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683006 sequences (100000248 bp) in 3.161 CPU sec, 3.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287381, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 302, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (319.17, 149.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683732 reads in 181.771 CPU sec, 5.736 real sec
[M::process] read 682898 sequences (100000189 bp) in 2.485 CPU sec, 2.487 real sec...
sorting block 78, #reads = 1085098 mem = 536881057 peak = 1093652480
[M::mem_process_seqs] Processed 683408 reads in 117.106 CPU sec, 3.683 real sec
sorting block 79, #reads = 1085227 mem = 536878943 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287484, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (317.93, 148.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682518 sequences (100000075 bp) in 3.342 CPU sec, 3.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287347, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (319.26, 148.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682442 sequences (100000178 bp) in 1.806 CPU sec, 1.804 real sec...
[M::mem_process_seqs] Processed 683006 reads in 189.508 CPU sec, 6.002 real sec
[M::mem_process_seqs] Processed 682898 reads in 123.275 CPU sec, 3.899 real sec
sorting block 80, #reads = 1084291 mem = 536878761 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287502, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (319.81, 147.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682626 sequences (100000149 bp) in 2.994 CPU sec, 3.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287469, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (319.91, 148.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682624 sequences (100000003 bp) in 2.210 CPU sec, 2.211 real sec...
[M::mem_process_seqs] Processed 682518 reads in 189.189 CPU sec, 5.985 real sec
[M::mem_process_seqs] Processed 682442 reads in 123.816 CPU sec, 3.916 real sec
sorting block 81, #reads = 1084275 mem = 536877620 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287470, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (318.72, 147.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682388 sequences (100000276 bp) in 2.753 CPU sec, 2.813 real sec...
[M::process] read 682294 sequences (100000130 bp) in 1.758 CPU sec, 1.766 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287095, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (317.86, 147.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682626 reads in 185.000 CPU sec, 5.871 real sec
sorting block 82, #reads = 1084195 mem = 536883927 peak = 1093652480
[M::mem_process_seqs] Processed 682624 reads in 122.454 CPU sec, 3.885 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287459, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 300, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (317.12, 145.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682190 sequences (100000106 bp) in 2.893 CPU sec, 2.939 real sec...
[M::process] read 682126 sequences (100000213 bp) in 1.850 CPU sec, 1.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286014, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (317.52, 146.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682388 reads in 187.620 CPU sec, 5.942 real sec
sorting block 83, #reads = 1084216 mem = 536884154 peak = 1093652480
[M::mem_process_seqs] Processed 682294 reads in 123.038 CPU sec, 3.902 real sec
sorting block 84, #reads = 1083283 mem = 536879103 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287340, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (319.14, 147.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682052 sequences (100000276 bp) in 2.705 CPU sec, 2.779 real sec...
[M::process] read 682174 sequences (100000054 bp) in 1.815 CPU sec, 1.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287547, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (317.39, 146.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682190 reads in 189.174 CPU sec, 6.005 real sec
[M::mem_process_seqs] Processed 682126 reads in 121.388 CPU sec, 3.867 real sec
sorting block 85, #reads = 1082955 mem = 536880540 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286962, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (318.15, 146.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681752 sequences (100000051 bp) in 3.110 CPU sec, 3.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286981, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (317.95, 145.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682082 sequences (100000210 bp) in 2.440 CPU sec, 2.449 real sec...
[M::mem_process_seqs] Processed 682052 reads in 193.616 CPU sec, 6.117 real sec
[M::mem_process_seqs] Processed 682174 reads in 126.756 CPU sec, 4.007 real sec
sorting block 86, #reads = 1081800 mem = 536879902 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287071, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (318.79, 145.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681746 sequences (100000089 bp) in 3.109 CPU sec, 3.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287360, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (318.82, 146.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681492 sequences (100000263 bp) in 2.270 CPU sec, 2.269 real sec...
[M::mem_process_seqs] Processed 681752 reads in 192.200 CPU sec, 6.077 real sec
sorting block 87, #reads = 1082591 mem = 536879223 peak = 1093652480
[M::mem_process_seqs] Processed 682082 reads in 124.736 CPU sec, 3.934 real sec
sorting block 88, #reads = 1083198 mem = 536878874 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286948, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (321.23, 147.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681642 sequences (100000090 bp) in 2.879 CPU sec, 2.926 real sec...
[M::process] read 690728 sequences (100000204 bp) in 2.060 CPU sec, 2.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286563, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (322.95, 149.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681746 reads in 193.808 CPU sec, 6.140 real sec
[M::mem_process_seqs] Processed 681492 reads in 126.505 CPU sec, 4.002 real sec
sorting block 89, #reads = 1081770 mem = 536881172 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286861, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (322.25, 148.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691388 sequences (100000171 bp) in 3.561 CPU sec, 3.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289715, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 263, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (285.28, 137.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691034 sequences (100000004 bp) in 2.470 CPU sec, 2.473 real sec...
[M::mem_process_seqs] Processed 681642 reads in 205.476 CPU sec, 6.488 real sec
[M::mem_process_seqs] Processed 690728 reads in 137.403 CPU sec, 4.334 real sec
sorting block 90, #reads = 1082155 mem = 536875842 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289606, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 260, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (282.12, 135.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689326 sequences (100000020 bp) in 3.157 CPU sec, 3.197 real sec...
[M::process] read 688368 sequences (100000042 bp) in 2.310 CPU sec, 2.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290004, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 261, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (282.88, 135.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691388 reads in 212.479 CPU sec, 6.727 real sec
[M::mem_process_seqs] Processed 691034 reads in 138.681 CPU sec, 4.397 real sec
sorting block 91, #reads = 1085388 mem = 536880008 peak = 1093652480
[M::process] read 687456 sequences (100000195 bp) in 2.692 CPU sec, 2.767 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289371, 4, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (608, 608, 2154)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5246)
[M::mem_pestat] mean and std.dev: (987.50, 715.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6792)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 269, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (288.84, 137.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687394 sequences (100000190 bp) in 1.810 CPU sec, 1.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289017, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 277, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (296.46, 139.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289510, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 275, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (293.75, 138.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689326 reads in 296.268 CPU sec, 9.461 real sec
sorting block 92, #reads = 1082348 mem = 536879064 peak = 1093652480
[M::mem_process_seqs] Processed 687456 reads in 222.614 CPU sec, 7.139 real sec
[M::mem_process_seqs] Processed 688368 reads in 244.645 CPU sec, 7.866 real sec
sorting block 93, #reads = 1081834 mem = 536879925 peak = 1093652480
[M::process] read 686394 sequences (100000234 bp) in 3.181 CPU sec, 3.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289186, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (299.07, 141.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686294 sequences (100000158 bp) in 2.572 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288563, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 286, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (303.79, 143.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687394 reads in 202.381 CPU sec, 6.438 real sec
[M::mem_process_seqs] Processed 686394 reads in 130.993 CPU sec, 4.122 real sec
sorting block 94, #reads = 1084438 mem = 536877089 peak = 1093652480
[M::process] read 686064 sequences (100000275 bp) in 2.961 CPU sec, 3.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288494, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (303.22, 142.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 95, #reads = 1083970 mem = 536881436 peak = 1093652480
[M::process] read 685182 sequences (100000241 bp) in 2.695 CPU sec, 2.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288214, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (304.39, 142.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686294 reads in 204.241 CPU sec, 6.457 real sec
[M::process] read 684664 sequences (100000118 bp) in 2.280 CPU sec, 2.280 real sec...
[M::mem_process_seqs] Processed 686064 reads in 134.834 CPU sec, 4.243 real sec
sorting block 96, #reads = 1082296 mem = 536880222 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 288013, 5, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (320, 388, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 680)
[M::mem_pestat] mean and std.dev: (346.67, 107.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (309.04, 144.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683846 sequences (100000102 bp) in 3.000 CPU sec, 3.105 real sec...
[M::process] read 683334 sequences (100000058 bp) in 2.030 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287830, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (311.79, 145.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685182 reads in 198.956 CPU sec, 6.300 real sec
[M::mem_process_seqs] Processed 684664 reads in 130.735 CPU sec, 4.150 real sec
sorting block 97, #reads = 1082632 mem = 536883488 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287858, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (315.27, 146.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683182 sequences (100000109 bp) in 2.676 CPU sec, 2.741 real sec...
[M::process] read 682922 sequences (100000242 bp) in 2.220 CPU sec, 2.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287730, 13, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (315.51, 146.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (527, 1662, 1967)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4847)
[M::mem_pestat] mean and std.dev: (1243.38, 748.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6287)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 683846 reads in 198.213 CPU sec, 6.283 real sec
[M::mem_process_seqs] Processed 683334 reads in 129.252 CPU sec, 4.096 real sec
sorting block 98, #reads = 1082163 mem = 536877920 peak = 1093652480
[M::process] read 682672 sequences (100000032 bp) in 3.308 CPU sec, 3.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287201, 11, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (315.30, 145.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1756, 1756, 1833)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1602, 1987)
[M::mem_pestat] mean and std.dev: (1781.67, 36.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1525, 2064)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 200, 983)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2549)
[M::mem_pestat] mean and std.dev: (431.90, 354.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3332)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682922 reads in 101.490 CPU sec, 3.190 real sec
[M::process] read 682520 sequences (100000167 bp) in 2.326 CPU sec, 2.335 real sec...
sorting block 99, #reads = 1082144 mem = 536879975 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287701, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (252, 400, 735)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1701)
[M::mem_pestat] mean and std.dev: (414.40, 238.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2184)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (315.73, 146.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287398, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (317.33, 146.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682318 sequences (100000105 bp) in 2.186 CPU sec, 2.188 real sec...
[M::mem_process_seqs] Processed 683182 reads in 289.470 CPU sec, 9.161 real sec
[M::mem_process_seqs] Processed 682672 reads in 143.785 CPU sec, 4.563 real sec
sorting block 100, #reads = 1082212 mem = 536882768 peak = 1093652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287154, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (317.94, 147.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682692 sequences (100000048 bp) in 2.758 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287035, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (317.87, 147.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682350 sequences (100000112 bp) in 2.340 CPU sec, 2.338 real sec...
[M::mem_process_seqs] Processed 682520 reads in 182.775 CPU sec, 5.788 real sec
sorting block 101, #reads = 1082768 mem = 536880084 peak = 1093652480
[M::mem_process_seqs] Processed 682318 reads in 117.548 CPU sec, 3.713 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287362, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 300, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (316.93, 146.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681668 sequences (100000009 bp) in 3.058 CPU sec, 3.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287243, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (317.45, 146.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682692 reads in 174.862 CPU sec, 5.510 real sec
[M::process] read 681698 sequences (100000269 bp) in 2.360 CPU sec, 2.351 real sec...
sorting block 102, #reads = 1083095 mem = 536878801 peak = 1093652480
[M::mem_process_seqs] Processed 682350 reads in 116.025 CPU sec, 3.658 real sec
sorting block 103, #reads = 1082328 mem = 536881461 peak = 1127424000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286494, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (320.48, 147.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681630 sequences (100000190 bp) in 2.591 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286922, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (320.95, 147.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 195, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (243.73, 121.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681668 reads in 168.409 CPU sec, 5.335 real sec
[M::process] read 681852 sequences (100000199 bp) in 2.514 CPU sec, 2.532 real sec...
[M::mem_process_seqs] Processed 681698 reads in 112.113 CPU sec, 3.524 real sec
sorting block 104, #reads = 1082277 mem = 536881850 peak = 1127424000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286896, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (321.80, 148.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682684 sequences (100000051 bp) in 2.650 CPU sec, 2.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287305, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (321.65, 149.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691178 sequences (100000267 bp) in 1.810 CPU sec, 1.811 real sec...
[M::mem_process_seqs] Processed 681630 reads in 170.810 CPU sec, 5.420 real sec
[M::mem_process_seqs] Processed 681852 reads in 114.863 CPU sec, 3.636 real sec
sorting block 105, #reads = 1084069 mem = 536877959 peak = 1127424000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286978, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (318.03, 149.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689034 sequences (100000022 bp) in 2.843 CPU sec, 2.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289517, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 262, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (283.12, 135.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688244 sequences (100000036 bp) in 2.340 CPU sec, 2.336 real sec...
[M::mem_process_seqs] Processed 682684 reads in 190.434 CPU sec, 6.023 real sec
sorting block 106, #reads = 1081095 mem = 536877717 peak = 1127424000
[M::mem_process_seqs] Processed 691178 reads in 123.214 CPU sec, 3.890 real sec
sorting block 107, #reads = 1081399 mem = 536878690 peak = 1127424000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288719, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 273, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (292.70, 139.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687424 sequences (100000173 bp) in 3.050 CPU sec, 3.111 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289036, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 275, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (294.47, 139.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689034 reads in 169.959 CPU sec, 5.366 real sec
[M::process] read 686716 sequences (100000263 bp) in 2.415 CPU sec, 2.433 real sec...
[M::mem_process_seqs] Processed 688244 reads in 111.379 CPU sec, 3.505 real sec
sorting block 108, #reads = 1088476 mem = 536877163 peak = 1127424000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288388, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 279, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (297.43, 140.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685998 sequences (100000031 bp) in 2.485 CPU sec, 2.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288209, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (300.17, 140.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687424 reads in 162.861 CPU sec, 5.164 real sec
[M::process] read 685658 sequences (100000191 bp) in 2.396 CPU sec, 2.405 real sec...
[M::mem_process_seqs] Processed 686716 reads in 109.223 CPU sec, 3.430 real sec
sorting block 109, #reads = 1087914 mem = 536877780 peak = 1127424000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288644, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.62, 140.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685194 sequences (100000261 bp) in 2.810 CPU sec, 2.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288476, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (304.40, 141.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685998 reads in 155.943 CPU sec, 4.921 real sec
[M::process] read 684858 sequences (100000193 bp) in 2.068 CPU sec, 2.078 real sec...
sorting block 110, #reads = 1087507 mem = 536880543 peak = 1127424000
[M::mem_process_seqs] Processed 685658 reads in 104.357 CPU sec, 3.281 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288373, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (307.41, 142.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685496 sequences (100000207 bp) in 2.717 CPU sec, 2.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287797, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (308.97, 143.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685194 reads in 166.346 CPU sec, 5.248 real sec
[M::process] read 684920 sequences (100000043 bp) in 2.278 CPU sec, 2.283 real sec...
sorting block 111, #reads = 1086772 mem = 536878630 peak = 1127424000
[M::mem_process_seqs] Processed 684858 reads in 109.679 CPU sec, 3.455 real sec
sorting block 112, #reads = 1084634 mem = 536879517 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288310, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (306.78, 142.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684758 sequences (100000288 bp) in 2.893 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288055, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (308.43, 142.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685496 reads in 158.435 CPU sec, 4.993 real sec
[M::process] read 684152 sequences (100000113 bp) in 2.162 CPU sec, 2.168 real sec...
[M::mem_process_seqs] Processed 684920 reads in 106.712 CPU sec, 3.378 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287907, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 330, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (257.60, 112.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 293, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (308.26, 141.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 113, #reads = 1086795 mem = 536879984 peak = 1132150784
[M::process] read 684948 sequences (100000258 bp) in 3.075 CPU sec, 3.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287653, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (309.85, 142.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684758 reads in 161.018 CPU sec, 5.139 real sec
[M::mem_process_seqs] Processed 684152 reads in 112.508 CPU sec, 3.557 real sec
[M::process] read 684114 sequences (100000241 bp) in 3.132 CPU sec, 3.376 real sec...
sorting block 114, #reads = 1086528 mem = 536879372 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288530, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 739, 3576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10382)
[M::mem_pestat] mean and std.dev: (1345.60, 1490.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (305.84, 140.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683174 sequences (100000097 bp) in 3.234 CPU sec, 3.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287613, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (311.16, 143.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684948 reads in 191.301 CPU sec, 6.115 real sec
[M::mem_process_seqs] Processed 684114 reads in 127.980 CPU sec, 4.052 real sec
sorting block 115, #reads = 1085255 mem = 536880044 peak = 1132150784
[M::process] read 683132 sequences (100000287 bp) in 3.349 CPU sec, 3.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287609, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (316.65, 145.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287209, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (315.68, 144.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682804 sequences (100000223 bp) in 3.715 CPU sec, 4.071 real sec...
[M::mem_process_seqs] Processed 683174 reads in 214.111 CPU sec, 6.927 real sec
[M::mem_process_seqs] Processed 683132 reads in 142.048 CPU sec, 4.566 real sec
sorting block 116, #reads = 1084454 mem = 536882796 peak = 1132150784
[M::process] read 682644 sequences (100000283 bp) in 3.857 CPU sec, 4.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287227, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (317.73, 146.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 117, #reads = 1083751 mem = 536878729 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287477, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 300, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (317.08, 146.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682804 reads in 221.449 CPU sec, 7.156 real sec
[M::process] read 683094 sequences (100000268 bp) in 3.843 CPU sec, 4.143 real sec...
[M::mem_process_seqs] Processed 682644 reads in 147.833 CPU sec, 4.738 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287748, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 296, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (313.45, 144.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 118, #reads = 1082435 mem = 536879079 peak = 1132150784
[M::process] read 682846 sequences (100000091 bp) in 3.841 CPU sec, 4.109 real sec...
[M::mem_process_seqs] Processed 683094 reads in 117.320 CPU sec, 3.864 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287379, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 297, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (313.25, 143.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 739, 943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413)
[M::mem_pestat] mean and std.dev: (597.80, 309.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3148)
[M::mem_pestat] skip orientation RR
[M::process] read 682490 sequences (100000202 bp) in 3.777 CPU sec, 4.060 real sec...
[M::mem_process_seqs] Processed 682846 reads in 117.365 CPU sec, 3.833 real sec
sorting block 119, #reads = 1081762 mem = 536877961 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286352, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 300, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (316.42, 145.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682490 reads in 115.962 CPU sec, 3.783 real sec
[M::process] read 682622 sequences (100000147 bp) in 3.868 CPU sec, 4.198 real sec...
sorting block 120, #reads = 1081695 mem = 536879743 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286785, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 299, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (316.28, 146.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682622 reads in 113.535 CPU sec, 3.675 real sec
[M::process] read 682474 sequences (100000136 bp) in 3.447 CPU sec, 3.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286930, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (317.48, 146.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682376 sequences (100000173 bp) in 3.027 CPU sec, 3.159 real sec...
[M::mem_process_seqs] Processed 682474 reads in 118.690 CPU sec, 3.822 real sec
sorting block 121, #reads = 1082354 mem = 536881730 peak = 1132150784
[M::process] read 682646 sequences (100000121 bp) in 3.380 CPU sec, 3.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287448, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (317.64, 146.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685220 sequences (100000278 bp) in 2.160 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287087, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 300, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (317.18, 146.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682376 reads in 211.873 CPU sec, 6.666 real sec
[M::mem_process_seqs] Processed 682646 reads in 143.059 CPU sec, 4.512 real sec
[M::process] read 693750 sequences (100000259 bp) in 2.636 CPU sec, 2.718 real sec...
sorting block 122, #reads = 1079157 mem = 536880837 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287550, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (306.98, 145.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691880 sequences (100000277 bp) in 3.246 CPU sec, 3.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 290718, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 252, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (274.40, 132.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690186 sequences (100000288 bp) in 2.302 CPU sec, 2.308 real sec...
[M::mem_process_seqs] Processed 685220 reads in 232.078 CPU sec, 7.389 real sec
sorting block 123, #reads = 1078276 mem = 536881622 peak = 1132150784
[M::mem_process_seqs] Processed 693750 reads in 149.328 CPU sec, 4.755 real sec
sorting block 124, #reads = 1076708 mem = 536879837 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290297, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 260, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (281.59, 134.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689460 sequences (100000019 bp) in 3.217 CPU sec, 3.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289819, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 267, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (286.79, 135.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688352 sequences (100000147 bp) in 2.500 CPU sec, 2.504 real sec...
[M::mem_process_seqs] Processed 691880 reads in 214.638 CPU sec, 6.826 real sec
[M::mem_process_seqs] Processed 690186 reads in 142.450 CPU sec, 4.531 real sec
sorting block 125, #reads = 1083354 mem = 536880355 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 289104, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 271, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (289.38, 135.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 501, 4383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12723)
[M::mem_pestat] mean and std.dev: (1967.75, 2358.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16893)
[M::mem_pestat] skip orientation RR
[M::process] read 687248 sequences (100000052 bp) in 3.549 CPU sec, 3.798 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288715, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 277, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (293.68, 136.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (296, 482, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (436.78, 233.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689460 reads in 220.445 CPU sec, 7.030 real sec
[M::mem_process_seqs] Processed 688352 reads in 145.576 CPU sec, 4.598 real sec
[M::process] read 687004 sequences (100000236 bp) in 2.957 CPU sec, 3.058 real sec...
sorting block 126, #reads = 1082765 mem = 536878028 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288547, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (298.88, 139.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685966 sequences (100000074 bp) in 3.655 CPU sec, 3.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288588, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 284, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (299.94, 139.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 354, 913)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2295)
[M::mem_pestat] mean and std.dev: (510.20, 335.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2986)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687248 reads in 216.073 CPU sec, 6.878 real sec
[M::process] read 686566 sequences (100000031 bp) in 2.395 CPU sec, 2.436 real sec...
[M::mem_process_seqs] Processed 687004 reads in 143.416 CPU sec, 4.534 real sec
sorting block 127, #reads = 1082102 mem = 536879252 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288601, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (305.36, 141.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 128, #reads = 1081439 mem = 536878829 peak = 1132150784
[M::process] read 685756 sequences (100000213 bp) in 3.383 CPU sec, 3.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288722, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 287, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (304.34, 142.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685966 reads in 216.204 CPU sec, 6.921 real sec
[M::mem_process_seqs] Processed 686566 reads in 147.768 CPU sec, 4.669 real sec
[M::process] read 685550 sequences (100000073 bp) in 2.930 CPU sec, 2.993 real sec...
sorting block 129, #reads = 1081929 mem = 536878275 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287909, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (306.05, 142.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685862 sequences (100000129 bp) in 2.690 CPU sec, 2.835 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288113, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 292, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.54, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685088 sequences (100000294 bp) in 2.478 CPU sec, 2.475 real sec...
[M::mem_process_seqs] Processed 685756 reads in 201.596 CPU sec, 6.399 real sec
[M::mem_process_seqs] Processed 685550 reads in 131.081 CPU sec, 4.139 real sec
sorting block 130, #reads = 1080149 mem = 536880242 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 288171, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (362, 1000, 1152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2732)
[M::mem_pestat] mean and std.dev: (816.83, 594.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3522)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (304.20, 142.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683816 sequences (100000269 bp) in 2.858 CPU sec, 2.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287623, 2, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (304.37, 141.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 634, 985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2509)
[M::mem_pestat] mean and std.dev: (614.47, 450.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3271)
[M::mem_pestat] skip orientation RR
[M::process] read 683184 sequences (100000149 bp) in 2.380 CPU sec, 2.385 real sec...
[M::mem_process_seqs] Processed 685862 reads in 190.556 CPU sec, 6.028 real sec
[M::mem_process_seqs] Processed 685088 reads in 130.394 CPU sec, 4.131 real sec
sorting block 131, #reads = 1083200 mem = 536882341 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287826, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (309.47, 141.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682714 sequences (100000126 bp) in 3.350 CPU sec, 3.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287653, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (312.80, 142.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683816 reads in 196.365 CPU sec, 6.223 real sec
[M::process] read 682744 sequences (100000043 bp) in 2.568 CPU sec, 2.573 real sec...
sorting block 132, #reads = 1079911 mem = 536878756 peak = 1132150784
[M::mem_process_seqs] Processed 683184 reads in 130.508 CPU sec, 4.116 real sec
sorting block 133, #reads = 1081323 mem = 536876868 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287270, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 299, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (313.20, 142.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682838 sequences (100000205 bp) in 2.568 CPU sec, 2.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287256, 10, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 298, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (312.66, 141.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1193, 1193, 1193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1193, 1193)
[M::mem_pestat] mean and std.dev: (1193.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1193, 1193)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 682714 reads in 191.232 CPU sec, 6.075 real sec
[M::process] read 682378 sequences (100000230 bp) in 2.475 CPU sec, 2.489 real sec...
[M::mem_process_seqs] Processed 682744 reads in 127.580 CPU sec, 4.027 real sec
sorting block 134, #reads = 1081700 mem = 536880218 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287401, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 296, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (311.72, 141.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682408 sequences (100000106 bp) in 3.196 CPU sec, 3.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286927, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 160, 160)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276)
[M::mem_pestat] mean and std.dev: (137.25, 28.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 334)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 295, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (309.87, 139.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 297, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (270.00, 118.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682838 reads in 187.142 CPU sec, 5.919 real sec
[M::process] read 682124 sequences (100000170 bp) in 2.522 CPU sec, 2.523 real sec...
[M::mem_process_seqs] Processed 682378 reads in 124.734 CPU sec, 3.924 real sec
sorting block 135, #reads = 1082277 mem = 536881975 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287279, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 298, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (312.85, 141.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681714 sequences (100000264 bp) in 2.985 CPU sec, 3.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287090, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 298, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (312.64, 141.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682408 reads in 181.533 CPU sec, 5.787 real sec
[M::mem_process_seqs] Processed 682124 reads in 122.122 CPU sec, 3.892 real sec
[M::process] read 681620 sequences (100000029 bp) in 2.658 CPU sec, 2.703 real sec...
sorting block 136, #reads = 1082014 mem = 536881102 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286929, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (317.72, 144.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681420 sequences (100000301 bp) in 3.269 CPU sec, 3.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287063, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (320.01, 146.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681714 reads in 185.955 CPU sec, 6.023 real sec
sorting block 137, #reads = 1083470 mem = 536879470 peak = 1132150784
[M::mem_process_seqs] Processed 681620 reads in 123.935 CPU sec, 3.977 real sec
[M::process] read 681286 sequences (100000138 bp) in 2.982 CPU sec, 3.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287248, 3, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 305, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (320.44, 146.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (377, 508, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (39, 884)
[M::mem_pestat] mean and std.dev: (430.77, 116.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RR
sorting block 138, #reads = 1083218 mem = 536878804 peak = 1132150784
[M::process] read 463588 sequences (68011811 bp) in 2.263 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286617, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (324.11, 149.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681420 reads in 184.498 CPU sec, 6.013 real sec
[M::mem_process_seqs] Processed 681286 reads in 125.354 CPU sec, 4.083 real sec
sorting block 139, #reads = 1083117 mem = 536880131 peak = 1132150784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 195154, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (320.69, 147.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 463588 reads in 76.905 CPU sec, 2.695 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 152195428
overall: 69971779584 mem 23150.741 user 567.378 sys 775.361 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cAd-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:659_cAd-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cAd-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cAd-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cAd-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 775.400 sec; CPU: 23718.125 sec
sorting block 140, #reads = 940413 mem = 467097875 peak = 1132150784
uniq header count 1
merging 141 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8125749899
output reads: 152786553
bam_mem_sort: 141 calls 82.071 user 2.026 sys 84.451 real
bam_write: 141 calls 47.460 user 3.586 sys 59.852 real
execute: 1 calls 62.346 user 3.936 sys 849.873 real
merge_files: 1 calls 62.304 user 3.912 sys 73.718 real
parse_chunk: 2254 calls 127.469 user 30.757 sys 163.592 real
read_chunk: 6076 calls 75.109 user 47.569 sys 755.868 real
sort_block: 1 calls 45.073 user 46.600 sys 716.294 real
write_chunk: 3820 calls 61.664 user 3.536 sys 72.188 real
overall: 3237044224 mem 2780.428 user 121.203 sys 849.895 real
ls: cannot access '*.cram': No such file or directory