cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1708.757 CPU sec, 1137.704 real sec
[M::process] read 687940 sequences (100000134 bp) in 1.009 CPU sec, 1.014 real sec...
[M::process] read 685718 sequences (100000007 bp) in 1.240 CPU sec, 1.237 real sec...
[M::process] read 684304 sequences (100000127 bp) in 1.170 CPU sec, 1.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284150, 9, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (285, 413, 960)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2310)
[M::mem_pestat] mean and std.dev: (361.43, 195.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2985)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 296, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (318.33, 160.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 305, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (278.75, 109.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683792 sequences (100000147 bp) in 1.129 CPU sec, 1.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283385, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 287, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (293.76, 142.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (334.16, 163.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 523, 827)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985)
[M::mem_pestat] mean and std.dev: (509.38, 388.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2564)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283783, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 230, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (265.10, 141.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 309, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (327.68, 162.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 332, 772)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1760)
[M::mem_pestat] mean and std.dev: (446.69, 306.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2254)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687940 reads in 231.124 CPU sec, 7.361 real sec
[M::mem_process_seqs] Processed 684304 reads in 148.083 CPU sec, 4.776 real sec
[M::mem_process_seqs] Processed 685718 reads in 160.149 CPU sec, 5.184 real sec
sorting block 0, #reads = 1081480 mem = 536882774 peak = 593481728
[M::process] read 683002 sequences (100000118 bp) in 1.783 CPU sec, 1.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283431, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (261, 337, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (318.23, 123.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (337.29, 165.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 214, 1191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3225)
[M::mem_pestat] mean and std.dev: (535.21, 750.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4242)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682618 sequences (100000054 bp) in 1.253 CPU sec, 1.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282490, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 328, 624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1316)
[M::mem_pestat] mean and std.dev: (417.64, 205.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1662)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (342.11, 167.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 353, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (275.89, 104.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682262 sequences (100000271 bp) in 1.110 CPU sec, 1.113 real sec...
[M::mem_process_seqs] Processed 683792 reads in 137.749 CPU sec, 4.378 real sec
sorting block 1, #reads = 1081358 mem = 536878346 peak = 755798016
[M::mem_process_seqs] Processed 683002 reads in 85.874 CPU sec, 2.715 real sec
sorting block 2, #reads = 1080348 mem = 536878299 peak = 917565440
[M::process] read 682790 sequences (100000047 bp) in 1.485 CPU sec, 1.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282385, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 209, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (242.79, 118.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (343.69, 167.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 159, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (208.19, 135.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682678 sequences (100000048 bp) in 1.220 CPU sec, 1.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282258, 6, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 310, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (88, 458)
[M::mem_pestat] mean and std.dev: (259.11, 87.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 610)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (341.42, 164.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556)
[M::mem_pestat] mean and std.dev: (224.17, 120.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282809, 4, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 199, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (220.31, 80.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 320, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (336.48, 162.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 501, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221)
[M::mem_pestat] mean and std.dev: (405.12, 194.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1554)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682618 reads in 200.383 CPU sec, 6.410 real sec
[M::mem_process_seqs] Processed 682262 reads in 148.317 CPU sec, 4.768 real sec
[M::mem_process_seqs] Processed 682790 reads in 160.453 CPU sec, 5.174 real sec
sorting block 3, #reads = 1079023 mem = 536881700 peak = 917565440
[M::process] read 682464 sequences (100000184 bp) in 1.848 CPU sec, 1.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282417, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 367, 2325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6487)
[M::mem_pestat] mean and std.dev: (878.15, 910.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 318, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (334.05, 160.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 226, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (272.70, 155.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 4, #reads = 1078434 mem = 536878857 peak = 917565440
[M::process] read 681846 sequences (100000266 bp) in 1.378 CPU sec, 1.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282927, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 288, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611)
[M::mem_pestat] mean and std.dev: (368.65, 263.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2096)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 320, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (336.17, 161.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 197, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (204.32, 109.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685902 sequences (100000062 bp) in 1.174 CPU sec, 1.182 real sec...
[M::mem_process_seqs] Processed 682678 reads in 142.628 CPU sec, 4.532 real sec
[M::mem_process_seqs] Processed 682464 reads in 88.209 CPU sec, 2.784 real sec
sorting block 5, #reads = 1079377 mem = 536877249 peak = 917565440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282177, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 243, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (249.07, 96.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 685726 sequences (100000099 bp) in 1.578 CPU sec, 1.676 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (341.87, 164.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 308, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546)
[M::mem_pestat] mean and std.dev: (263.61, 72.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684518 sequences (100000126 bp) in 1.220 CPU sec, 1.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283823, 3, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 272, 780)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882)
[M::mem_pestat] mean and std.dev: (443.07, 306.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2433)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 307, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (326.77, 163.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 1034)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2596)
[M::mem_pestat] mean and std.dev: (699.95, 622.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3377)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681846 reads in 139.595 CPU sec, 4.449 real sec
[M::mem_process_seqs] Processed 685902 reads in 86.136 CPU sec, 2.759 real sec
sorting block 6, #reads = 1077287 mem = 536878692 peak = 917565440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283666, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 377, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (318.33, 156.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (329.47, 163.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 242, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134)
[M::mem_pestat] mean and std.dev: (334.12, 238.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1457)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683858 sequences (100000294 bp) in 1.583 CPU sec, 1.677 real sec...
[M::process] read 683400 sequences (100000278 bp) in 1.220 CPU sec, 1.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283715, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 484, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117)
[M::mem_pestat] mean and std.dev: (435.18, 279.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1552)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (333.69, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 219, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (304.04, 139.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685726 reads in 137.635 CPU sec, 4.386 real sec
[M::mem_process_seqs] Processed 684518 reads in 85.624 CPU sec, 2.728 real sec
sorting block 7, #reads = 1082460 mem = 536878985 peak = 917565440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282843, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 212, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (243.64, 139.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (338.06, 165.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 345, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (361.22, 218.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682486 sequences (100000078 bp) in 1.897 CPU sec, 1.985 real sec...
[M::process] read 682194 sequences (100000093 bp) in 1.260 CPU sec, 1.269 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282833, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 231, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (231.47, 81.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (340.21, 165.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 230, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (255.00, 101.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683858 reads in 137.537 CPU sec, 4.374 real sec
sorting block 8, #reads = 1081020 mem = 536878335 peak = 917565440
[M::mem_process_seqs] Processed 683400 reads in 85.156 CPU sec, 2.710 real sec
sorting block 9, #reads = 1081220 mem = 536878587 peak = 917565440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282520, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (252, 264, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (326.45, 154.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (345.11, 167.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 323, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (342.28, 97.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682390 sequences (100000266 bp) in 1.727 CPU sec, 1.838 real sec...
[M::process] read 682438 sequences (100000239 bp) in 1.310 CPU sec, 1.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282604, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 226, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (241.58, 96.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (342.77, 164.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (310, 343, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546)
[M::mem_pestat] mean and std.dev: (455.07, 237.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1958)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682486 reads in 138.978 CPU sec, 4.428 real sec
[M::mem_process_seqs] Processed 682194 reads in 86.995 CPU sec, 2.760 real sec
sorting block 10, #reads = 1079493 mem = 536880969 peak = 917565440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282704, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 384, 1509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4161)
[M::mem_pestat] mean and std.dev: (599.75, 550.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5487)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (338.15, 161.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 251, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1817)
[M::mem_pestat] mean and std.dev: (400.50, 336.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2357)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682380 sequences (100000242 bp) in 2.054 CPU sec, 2.190 real sec...
[M::process] read 682470 sequences (100000283 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282727, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (396, 467, 709)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1335)
[M::mem_pestat] mean and std.dev: (551.17, 278.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (336.55, 161.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 352, 687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671)
[M::mem_pestat] mean and std.dev: (431.27, 297.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2163)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682390 reads in 140.381 CPU sec, 4.464 real sec
[M::mem_process_seqs] Processed 682438 reads in 87.650 CPU sec, 2.783 real sec
sorting block 11, #reads = 1078760 mem = 536878424 peak = 917565440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282997, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 546, 712)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1750)
[M::mem_pestat] mean and std.dev: (395.92, 249.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2269)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (338.48, 162.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 320, 949)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449)
[M::mem_pestat] mean and std.dev: (500.35, 429.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3199)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684958 sequences (100000171 bp) in 1.880 CPU sec, 1.996 real sec...
[M::process] read 686848 sequences (100000070 bp) in 1.310 CPU sec, 1.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282872, 8, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 258, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (320.20, 163.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (340.57, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 274, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (297.28, 141.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682380 reads in 139.606 CPU sec, 4.447 real sec
sorting block 12, #reads = 1079343 mem = 536879517 peak = 917565440
[M::mem_process_seqs] Processed 682470 reads in 87.631 CPU sec, 2.783 real sec
sorting block 13, #reads = 1077759 mem = 536877354 peak = 917565440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283626, 2, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 187, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (194.05, 87.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 313, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (331.27, 163.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (293, 321, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 572)
[M::mem_pestat] mean and std.dev: (332.27, 101.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686068 sequences (100000081 bp) in 2.075 CPU sec, 2.187 real sec...
[M::process] read 685050 sequences (100000238 bp) in 1.340 CPU sec, 1.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284286, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 203, 602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454)
[M::mem_pestat] mean and std.dev: (224.50, 121.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1880)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 301, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (321.49, 161.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (506, 859, 1553)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3647)
[M::mem_pestat] mean and std.dev: (1000.00, 742.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4694)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684958 reads in 142.704 CPU sec, 4.536 real sec
[M::mem_process_seqs] Processed 686848 reads in 87.614 CPU sec, 2.791 real sec
sorting block 14, #reads = 1080000 mem = 536884102 peak = 917565440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283648, 4, 22)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 307, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (326.44, 162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 325, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1414)
[M::mem_pestat] mean and std.dev: (377.45, 285.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1816)
[M::mem_pestat] skip orientation RR
[M::process] read 683730 sequences (100000072 bp) in 1.821 CPU sec, 1.920 real sec...
[M::process] read 683472 sequences (100000226 bp) in 1.320 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283590, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 248, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (132, 422)
[M::mem_pestat] mean and std.dev: (265.45, 44.44)
[M::mem_pestat] low and high boundaries for proper pairs: (74, 480)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 315, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (332.38, 164.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 444, 750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1978)
[M::mem_pestat] mean and std.dev: (412.70, 282.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2592)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686068 reads in 139.393 CPU sec, 4.442 real sec
[M::mem_process_seqs] Processed 685050 reads in 86.961 CPU sec, 2.757 real sec
sorting block 15, #reads = 1081969 mem = 536878550 peak = 917565440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283517, 6, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 356, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1820)
[M::mem_pestat] mean and std.dev: (506.43, 432.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2363)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (337.62, 165.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 416, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1818)
[M::mem_pestat] mean and std.dev: (548.90, 446.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2347)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683398 sequences (100000063 bp) in 2.184 CPU sec, 2.286 real sec...
[M::process] read 683856 sequences (100000253 bp) in 1.320 CPU sec, 1.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282782, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 288, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (275.88, 149.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (341.01, 168.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 407, 686)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1492)
[M::mem_pestat] mean and std.dev: (460.86, 269.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1895)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683730 reads in 140.427 CPU sec, 4.471 real sec
[M::mem_process_seqs] Processed 683472 reads in 88.015 CPU sec, 2.795 real sec
sorting block 16, #reads = 1080192 mem = 536879713 peak = 917565440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282799, 1, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 234, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 493)
[M::mem_pestat] mean and std.dev: (243.11, 56.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 587)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (340.04, 166.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 226, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (201.68, 83.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682222 sequences (100000171 bp) in 1.974 CPU sec, 2.115 real sec...
[M::process] read 682286 sequences (100000073 bp) in 1.330 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283178, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 245, 895)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2207)
[M::mem_pestat] mean and std.dev: (482.29, 312.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2863)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (333.17, 162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 214, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204)
[M::mem_pestat] mean and std.dev: (304.69, 201.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1552)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683398 reads in 143.154 CPU sec, 4.565 real sec
sorting block 17, #reads = 1080526 mem = 536877974 peak = 917565440
[M::mem_process_seqs] Processed 683856 reads in 88.974 CPU sec, 2.823 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281952, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 375, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133)
[M::mem_pestat] mean and std.dev: (337.50, 164.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1447)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (342.75, 165.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 303, 1841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5203)
[M::mem_pestat] mean and std.dev: (663.25, 691.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6884)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1079509 mem = 536877357 peak = 924602368
[M::process] read 682684 sequences (100000239 bp) in 1.986 CPU sec, 2.113 real sec...
[M::process] read 683654 sequences (100000032 bp) in 1.350 CPU sec, 1.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282487, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 269, 833)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2139)
[M::mem_pestat] mean and std.dev: (419.53, 329.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2792)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (342.90, 166.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 307, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 604)
[M::mem_pestat] mean and std.dev: (299.40, 72.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682222 reads in 145.477 CPU sec, 4.643 real sec
[M::mem_process_seqs] Processed 682286 reads in 90.442 CPU sec, 2.871 real sec
sorting block 19, #reads = 1078855 mem = 536877102 peak = 924602368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282623, 3, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 295, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (279.33, 105.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (341.38, 166.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 279, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1305)
[M::mem_pestat] mean and std.dev: (334.57, 198.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1685)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687252 sequences (100000150 bp) in 1.894 CPU sec, 1.995 real sec...
[M::process] read 686092 sequences (100000261 bp) in 1.310 CPU sec, 1.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283187, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 196, 1183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3183)
[M::mem_pestat] mean and std.dev: (550.38, 460.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4183)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (340.05, 168.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (295.94, 132.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682684 reads in 144.849 CPU sec, 4.625 real sec
[M::mem_process_seqs] Processed 683654 reads in 90.448 CPU sec, 2.876 real sec
sorting block 20, #reads = 1077364 mem = 536878891 peak = 924602368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283997, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 250, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1304)
[M::mem_pestat] mean and std.dev: (287.25, 180.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1691)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 301, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (322.11, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 304, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164)
[M::mem_pestat] mean and std.dev: (309.13, 134.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1474)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685640 sequences (100000085 bp) in 2.145 CPU sec, 2.245 real sec...
[M::process] read 685152 sequences (100000045 bp) in 1.310 CPU sec, 1.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284002, 1, 21)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 305, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (325.40, 162.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 308, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (376.86, 223.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687252 reads in 142.070 CPU sec, 4.526 real sec
[M::mem_process_seqs] Processed 686092 reads in 87.048 CPU sec, 2.767 real sec
sorting block 21, #reads = 1080601 mem = 536879667 peak = 924602368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283864, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 293, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443)
[M::mem_pestat] mean and std.dev: (314.48, 199.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1864)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 311, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (329.51, 164.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 374, 1163)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2947)
[M::mem_pestat] mean and std.dev: (549.47, 404.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3839)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684100 sequences (100000196 bp) in 1.937 CPU sec, 2.072 real sec...
[M::process] read 683800 sequences (100000055 bp) in 1.320 CPU sec, 1.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283437, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 264, 796)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024)
[M::mem_pestat] mean and std.dev: (285.09, 174.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2638)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (332.57, 164.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 456, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132)
[M::mem_pestat] mean and std.dev: (390.90, 150.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1431)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685640 reads in 142.832 CPU sec, 4.558 real sec
sorting block 22, #reads = 1083460 mem = 536880468 peak = 924602368
[M::process] read 683134 sequences (100000212 bp) in 1.623 CPU sec, 1.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283286, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (327, 385, 838)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1860)
[M::mem_pestat] mean and std.dev: (387.79, 204.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2371)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (338.19, 167.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 478, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1948)
[M::mem_pestat] mean and std.dev: (391.58, 285.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2538)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684100 reads in 83.050 CPU sec, 2.868 real sec
sorting block 23, #reads = 1080830 mem = 536879737 peak = 995983360
[M::mem_process_seqs] Processed 685152 reads in 197.152 CPU sec, 6.506 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282588, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 239, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (249.78, 126.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (340.77, 168.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 464, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2139)
[M::mem_pestat] mean and std.dev: (579.81, 413.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2787)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682584 sequences (100000008 bp) in 1.956 CPU sec, 2.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282716, 5, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 277, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (237.62, 102.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (340.59, 165.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 271, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (306.69, 138.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681680 sequences (100000252 bp) in 1.320 CPU sec, 1.319 real sec...
[M::mem_process_seqs] Processed 683800 reads in 157.108 CPU sec, 4.999 real sec
sorting block 24, #reads = 1080924 mem = 536878754 peak = 995983360
[M::mem_process_seqs] Processed 683134 reads in 90.354 CPU sec, 2.866 real sec
sorting block 25, #reads = 1080850 mem = 536881766 peak = 995983360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282506, 8, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 203, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (203.43, 49.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 595)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (342.75, 165.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 387, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165)
[M::mem_pestat] mean and std.dev: (342.65, 137.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1473)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681576 sequences (100000161 bp) in 2.171 CPU sec, 2.298 real sec...
[M::process] read 681672 sequences (100000213 bp) in 1.350 CPU sec, 1.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282043, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (276, 335, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1359)
[M::mem_pestat] mean and std.dev: (446.75, 354.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1867)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (344.62, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 371, 592)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296)
[M::mem_pestat] mean and std.dev: (429.45, 247.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1648)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682584 reads in 144.972 CPU sec, 4.616 real sec
[M::mem_process_seqs] Processed 681680 reads in 89.930 CPU sec, 2.857 real sec
sorting block 26, #reads = 1078582 mem = 536880902 peak = 995983360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282165, 8, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (395, 515, 1079)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2447)
[M::mem_pestat] mean and std.dev: (576.69, 311.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3131)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (343.02, 163.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 358, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (304.90, 128.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690822 sequences (100000296 bp) in 1.897 CPU sec, 1.992 real sec...
[M::process] read 688644 sequences (100000294 bp) in 1.410 CPU sec, 1.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282717, 10, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 361, 776)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852)
[M::mem_pestat] mean and std.dev: (489.76, 380.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2390)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (342.99, 164.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (293, 7065, 7065)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20609)
[M::mem_pestat] mean and std.dev: (3775.80, 3298.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 27381)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 286, 704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1578)
[M::mem_pestat] mean and std.dev: (421.91, 288.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2015)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681576 reads in 143.748 CPU sec, 4.584 real sec
[M::mem_process_seqs] Processed 681672 reads in 89.867 CPU sec, 2.852 real sec
sorting block 27, #reads = 1077185 mem = 536879694 peak = 995983360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286000, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 239, 952)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2492)
[M::mem_pestat] mean and std.dev: (455.15, 588.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3262)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 277, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (306.09, 158.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 287, 897)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2197)
[M::mem_pestat] mean and std.dev: (730.88, 686.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3475)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686092 sequences (100000233 bp) in 2.119 CPU sec, 2.206 real sec...
[M::process] read 685378 sequences (100000127 bp) in 1.490 CPU sec, 1.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285146, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 189, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (197.36, 104.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 292, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (317.05, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 227, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (237.29, 111.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690822 reads in 149.112 CPU sec, 4.742 real sec
[M::mem_process_seqs] Processed 688644 reads in 91.429 CPU sec, 2.913 real sec
sorting block 28, #reads = 1076907 mem = 536877981 peak = 995983360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285055, 8, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (262, 372, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156)
[M::mem_pestat] mean and std.dev: (361.35, 146.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1454)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 308, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (328.80, 164.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (288, 497, 2091)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5697)
[M::mem_pestat] mean and std.dev: (1014.48, 1162.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7500)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684292 sequences (100000095 bp) in 2.205 CPU sec, 2.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283734, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 236, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (275.17, 121.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 315, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (333.91, 166.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 248, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (256.22, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684144 sequences (100000024 bp) in 1.520 CPU sec, 1.521 real sec...
[M::mem_process_seqs] Processed 686092 reads in 144.524 CPU sec, 4.603 real sec
sorting block 29, #reads = 1080107 mem = 536879065 peak = 995983360
[M::mem_process_seqs] Processed 685378 reads in 91.555 CPU sec, 2.897 real sec
sorting block 30, #reads = 1079859 mem = 536878344 peak = 1056141312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283843, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (294.00, 135.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 322, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (339.99, 168.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 264, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (295.64, 110.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683314 sequences (100000156 bp) in 2.434 CPU sec, 2.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283708, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 420, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (312.12, 133.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 321, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (339.10, 167.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 310, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (362.28, 215.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1389)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683354 sequences (100000131 bp) in 1.510 CPU sec, 1.509 real sec...
[M::mem_process_seqs] Processed 684292 reads in 149.252 CPU sec, 4.746 real sec
[M::mem_process_seqs] Processed 684144 reads in 94.224 CPU sec, 2.986 real sec
sorting block 31, #reads = 1078641 mem = 536878359 peak = 1056141312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283039, 6, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 253, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073)
[M::mem_pestat] mean and std.dev: (363.07, 242.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (339.47, 165.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 437, 629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337)
[M::mem_pestat] mean and std.dev: (429.55, 218.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1691)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683390 sequences (100000068 bp) in 2.250 CPU sec, 2.395 real sec...
[M::process] read 682920 sequences (100000029 bp) in 1.480 CPU sec, 1.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282972, 9, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 492, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1403)
[M::mem_pestat] mean and std.dev: (506.62, 323.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1801)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (339.46, 165.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 341, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191)
[M::mem_pestat] mean and std.dev: (395.63, 267.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1515)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683314 reads in 150.361 CPU sec, 4.784 real sec
[M::mem_process_seqs] Processed 683354 reads in 94.368 CPU sec, 2.990 real sec
sorting block 32, #reads = 1076472 mem = 536879307 peak = 1056141312
[M::process] read 688382 sequences (100000196 bp) in 2.590 CPU sec, 2.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283108, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (272, 374, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (337.58, 139.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (338.98, 164.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (105, 186, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (277.22, 215.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1645)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 691564 sequences (100000092 bp) in 1.530 CPU sec, 1.524 real sec...
[M::mem_process_seqs] Processed 682920 reads in 86.026 CPU sec, 2.715 real sec
sorting block 33, #reads = 1075623 mem = 536878848 peak = 1056141312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282985, 7, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 287, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (263.00, 106.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (337.14, 164.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 286, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (254.00, 81.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285351, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 339, 887)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2177)
[M::mem_pestat] mean and std.dev: (478.08, 286.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2822)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 291, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (314.74, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 353, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1599)
[M::mem_pestat] mean and std.dev: (396.75, 200.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2049)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689476 sequences (100000208 bp) in 1.726 CPU sec, 1.732 real sec...
[M::mem_process_seqs] Processed 683390 reads in 232.600 CPU sec, 7.387 real sec
[M::mem_process_seqs] Processed 688382 reads in 112.045 CPU sec, 3.568 real sec
sorting block 34, #reads = 1074899 mem = 536880945 peak = 1056141312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286486, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 355, 1017)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2655)
[M::mem_pestat] mean and std.dev: (585.08, 505.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3474)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 275, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (300.42, 151.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 262, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (335.52, 202.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1378)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687178 sequences (100000241 bp) in 2.028 CPU sec, 2.140 real sec...
[M::process] read 685954 sequences (100000296 bp) in 1.340 CPU sec, 1.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 286151, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (292, 382, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (364.71, 111.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 287, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (308.75, 153.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 291, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (303.28, 137.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 691564 reads in 155.483 CPU sec, 4.961 real sec
sorting block 35, #reads = 1074229 mem = 536878794 peak = 1056141312
[M::mem_process_seqs] Processed 689476 reads in 97.143 CPU sec, 3.098 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 285339, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 273, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (331.12, 237.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 299, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (318.69, 157.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 562, 820)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1944)
[M::mem_pestat] mean and std.dev: (638.06, 500.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2639)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685564 sequences (100000093 bp) in 2.234 CPU sec, 2.369 real sec...
[M::process] read 684080 sequences (100000006 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284890, 6, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 341, 568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322)
[M::mem_pestat] mean and std.dev: (404.96, 284.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1699)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 306, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (324.80, 160.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 430, 1134)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2920)
[M::mem_pestat] mean and std.dev: (417.82, 274.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3813)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687178 reads in 149.139 CPU sec, 4.740 real sec
sorting block 36, #reads = 1075383 mem = 536879203 peak = 1056141312
[M::mem_process_seqs] Processed 685954 reads in 92.726 CPU sec, 2.948 real sec
sorting block 37, #reads = 1076541 mem = 536875862 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283666, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 302, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434)
[M::mem_pestat] mean and std.dev: (335.93, 223.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1862)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 309, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (329.03, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 474, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116)
[M::mem_pestat] mean and std.dev: (390.59, 219.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1416)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683602 sequences (100000300 bp) in 1.928 CPU sec, 2.045 real sec...
[M::process] read 683278 sequences (100000272 bp) in 1.370 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284191, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 233, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1182)
[M::mem_pestat] mean and std.dev: (238.59, 127.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 318, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (335.42, 164.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 289, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210)
[M::mem_pestat] mean and std.dev: (360.04, 238.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1552)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685564 reads in 148.881 CPU sec, 4.742 real sec
[M::mem_process_seqs] Processed 684080 reads in 92.617 CPU sec, 2.948 real sec
sorting block 38, #reads = 1076803 mem = 536880594 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283454, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 322, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (285.80, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (339.37, 167.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 322, 665)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1553)
[M::mem_pestat] mean and std.dev: (385.53, 255.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1997)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683160 sequences (100000098 bp) in 2.206 CPU sec, 2.329 real sec...
[M::process] read 688482 sequences (100000074 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283134, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 403, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (306.05, 156.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 323, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (342.33, 169.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 400, 593)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303)
[M::mem_pestat] mean and std.dev: (443.57, 283.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1658)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683602 reads in 146.613 CPU sec, 4.658 real sec
[M::mem_process_seqs] Processed 683278 reads in 91.435 CPU sec, 2.901 real sec
sorting block 39, #reads = 1075612 mem = 536878564 peak = 1102454784
[M::process] read 689482 sequences (100000051 bp) in 1.983 CPU sec, 2.120 real sec...
[M::process] read 687346 sequences (100000007 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284462, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 287, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (286.53, 112.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 291, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (316.87, 162.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 260, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (303.92, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688482 reads in 87.858 CPU sec, 2.793 real sec
sorting block 40, #reads = 1073400 mem = 536882670 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283300, 6, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 223, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (328.73, 192.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 325, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (344.76, 171.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 503, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367)
[M::mem_pestat] mean and std.dev: (414.13, 245.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1765)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285929, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 270, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (289.72, 150.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 287, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (312.84, 160.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 220, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (223.00, 87.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685816 sequences (100000083 bp) in 1.555 CPU sec, 1.556 real sec...
[M::mem_process_seqs] Processed 683160 reads in 229.541 CPU sec, 7.313 real sec
[M::mem_process_seqs] Processed 689482 reads in 105.423 CPU sec, 3.369 real sec
sorting block 41, #reads = 1076534 mem = 536879277 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284857, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 323, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (384.13, 258.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1418)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 298, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (321.13, 162.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 248, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (239.29, 93.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685640 sequences (100000241 bp) in 2.178 CPU sec, 2.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284392, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 305, 654)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614)
[M::mem_pestat] mean and std.dev: (436.00, 367.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2094)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 306, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (326.79, 163.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 300, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (281.89, 76.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685288 sequences (100000282 bp) in 1.310 CPU sec, 1.311 real sec...
[M::mem_process_seqs] Processed 687346 reads in 138.818 CPU sec, 4.416 real sec
[M::mem_process_seqs] Processed 685816 reads in 86.193 CPU sec, 2.736 real sec
sorting block 42, #reads = 1079247 mem = 536886224 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284040, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 768, 1694)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4674)
[M::mem_pestat] mean and std.dev: (1257.14, 1335.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6601)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 307, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (325.98, 161.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (300.15, 166.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 43, #reads = 1080489 mem = 536879753 peak = 1102454784
[M::process] read 686144 sequences (100000228 bp) in 1.861 CPU sec, 1.988 real sec...
[M::process] read 685168 sequences (100000077 bp) in 1.390 CPU sec, 1.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283868, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 329, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104)
[M::mem_pestat] mean and std.dev: (305.64, 128.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 309, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (328.47, 162.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 351, 690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1706)
[M::mem_pestat] mean and std.dev: (381.75, 226.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2214)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685640 reads in 140.740 CPU sec, 4.482 real sec
[M::mem_process_seqs] Processed 685288 reads in 88.613 CPU sec, 2.812 real sec
sorting block 44, #reads = 1078620 mem = 536878147 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284468, 1, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 221, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290)
[M::mem_pestat] mean and std.dev: (363.50, 257.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1664)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 304, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (323.64, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 245, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (245.63, 102.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684058 sequences (100000226 bp) in 2.180 CPU sec, 2.289 real sec...
[M::process] read 684026 sequences (100000024 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283715, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 205, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (235.58, 111.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 309, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (330.06, 165.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 303, 998)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2608)
[M::mem_pestat] mean and std.dev: (619.90, 615.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3413)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686144 reads in 143.259 CPU sec, 4.553 real sec
[M::mem_process_seqs] Processed 685168 reads in 90.305 CPU sec, 2.863 real sec
sorting block 45, #reads = 1078302 mem = 536881901 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283473, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 538, 1277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3325)
[M::mem_pestat] mean and std.dev: (726.54, 599.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4349)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 318, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (337.84, 168.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 240, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (231.55, 85.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687200 sequences (100000153 bp) in 1.850 CPU sec, 1.952 real sec...
[M::process] read 693332 sequences (100000069 bp) in 1.430 CPU sec, 1.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283633, 2, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 621, 705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663)
[M::mem_pestat] mean and std.dev: (482.15, 323.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2142)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 319, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (338.96, 169.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 209, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (257.00, 138.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684058 reads in 145.447 CPU sec, 4.628 real sec
[M::mem_process_seqs] Processed 684026 reads in 91.728 CPU sec, 2.917 real sec
sorting block 46, #reads = 1076206 mem = 536877266 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284954, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 278, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (311.48, 172.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 300, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (324.54, 166.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 208, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (184.94, 71.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 614)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 691748 sequences (100000242 bp) in 2.286 CPU sec, 2.398 real sec...
[M::process] read 688906 sequences (100000112 bp) in 1.400 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 287079, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 264, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (295.79, 160.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (293.52, 149.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 445, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1988)
[M::mem_pestat] mean and std.dev: (446.14, 388.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2587)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687200 reads in 157.686 CPU sec, 5.015 real sec
sorting block 47, #reads = 1075943 mem = 536880077 peak = 1102454784
[M::mem_process_seqs] Processed 693332 reads in 98.481 CPU sec, 3.135 real sec
sorting block 48, #reads = 1073728 mem = 536879429 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286455, 1, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (280, 286, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207)
[M::mem_pestat] mean and std.dev: (416.93, 265.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1516)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 273, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (297.98, 149.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 424, 572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298)
[M::mem_pestat] mean and std.dev: (347.14, 150.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1661)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687168 sequences (100000199 bp) in 1.868 CPU sec, 1.973 real sec...
[M::process] read 686582 sequences (100000262 bp) in 1.390 CPU sec, 1.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285244, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 297, 796)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112)
[M::mem_pestat] mean and std.dev: (409.96, 393.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2770)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 289, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (310.55, 154.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 245, 678)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744)
[M::mem_pestat] mean and std.dev: (517.53, 511.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2562)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 691748 reads in 153.225 CPU sec, 4.889 real sec
[M::mem_process_seqs] Processed 688906 reads in 94.966 CPU sec, 3.021 real sec
sorting block 49, #reads = 1075451 mem = 536879753 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285006, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 278, 906)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2242)
[M::mem_pestat] mean and std.dev: (490.81, 344.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2910)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 299, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (319.42, 158.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 195, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (228.18, 71.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 634)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685354 sequences (100000143 bp) in 2.287 CPU sec, 2.433 real sec...
[M::process] read 684702 sequences (100000117 bp) in 1.370 CPU sec, 1.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284126, 5, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 431, 599)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1359)
[M::mem_pestat] mean and std.dev: (448.34, 287.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1739)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 303, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (324.62, 161.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 349, 1781)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4765)
[M::mem_pestat] mean and std.dev: (875.08, 1058.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6257)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687168 reads in 147.628 CPU sec, 4.695 real sec
[M::mem_process_seqs] Processed 686582 reads in 93.164 CPU sec, 2.962 real sec
sorting block 50, #reads = 1076803 mem = 536878728 peak = 1102454784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284142, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 239, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (89, 464)
[M::mem_pestat] mean and std.dev: (231.60, 64.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 539)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 311, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (329.93, 163.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 294, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (53, 538)
[M::mem_pestat] mean and std.dev: (257.13, 70.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 635)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683394 sequences (100000194 bp) in 1.895 CPU sec, 1.981 real sec...
[M::process] read 683076 sequences (100000007 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283430, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 253, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133)
[M::mem_pestat] mean and std.dev: (346.00, 218.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1451)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 316, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (332.68, 163.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 421, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300)
[M::mem_pestat] mean and std.dev: (412.83, 203.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1645)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685354 reads in 145.667 CPU sec, 4.643 real sec
[M::mem_process_seqs] Processed 684702 reads in 90.761 CPU sec, 2.881 real sec
sorting block 51, #reads = 1077041 mem = 536880234 peak = 1102454784
[M::process] read 251700 sequences (36835969 bp) in 1.229 CPU sec, 1.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283104, 2, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 307, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (269.36, 129.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1344)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 325, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (340.32, 165.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 326, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151)
[M::mem_pestat] mean and std.dev: (318.00, 150.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1459)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282864, 15, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 313, 1065)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2797)
[M::mem_pestat] mean and std.dev: (599.65, 478.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3663)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (339.51, 165.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 180, 1138)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3072)
[M::mem_pestat] mean and std.dev: (605.14, 717.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4039)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 274, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (288.59, 136.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 104309, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (384, 384, 1200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2832)
[M::mem_pestat] mean and std.dev: (508.88, 281.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3648)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 318, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (336.03, 164.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 683394 reads in 163.473 CPU sec, 5.277 real sec
sorting block 52, #reads = 1076612 mem = 536878256 peak = 1113169920
[M::mem_process_seqs] Processed 683076 reads in 110.503 CPU sec, 3.571 real sec
[M::mem_process_seqs] Processed 251700 reads in 115.446 CPU sec, 3.741 real sec
sorting block 53, #reads = 1076381 mem = 536880254 peak = 1214713856
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 58467284
overall: 69901991936 mem 8142.729 user 296.929 sys 1361.094 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1361.133 sec; CPU: 8439.661 sec
sorting block 54, #reads = 545747 mem = 272309518 peak = 1214713856
uniq header count 1
merging 55 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3563793204
output reads: 58779789
bam_mem_sort: 55 calls 20.724 user 0.171 sys 21.018 real
bam_write: 55 calls 37.464 user 0.648 sys 38.417 real
execute: 1 calls 10.733 user 0.686 sys 1381.551 real
merge_files: 1 calls 10.723 user 0.658 sys 19.705 real
parse_chunk: 873 calls 28.207 user 7.577 sys 36.316 real
read_chunk: 2356 calls 21.174 user 19.727 sys 222.688 real
sort_block: 1 calls 16.793 user 19.207 sys 215.750 real
write_chunk: 1481 calls 10.375 user 0.490 sys 18.985 real
overall: 2723676160 mem 738.432 user 34.850 sys 1381.575 real
ls: cannot access '*.cram': No such file or directory