Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/47/a5c0aaabf5db47f9acc641be75db03/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/53/5dedb335f63aaa51af226d5f1f79b3/659_Kh-T1-TDNA-1_A23MHWWLT4_1/659_Kh-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/47/a5c0aaabf5db47f9acc641be75db03/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/53/5dedb335f63aaa51af226d5f1f79b3/659_Kh-T1-TDNA-1_A23MHWWLT4_1/659_Kh-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_Kh-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2426.994 CPU sec, 97.982 real sec
[M::process] read 670948 sequences (100000107 bp) in 1.260 CPU sec, 1.269 real sec...
[M::process] read 669264 sequences (100000050 bp) in 1.561 CPU sec, 1.590 real sec...
[M::process] read 669660 sequences (100000181 bp) in 1.721 CPU sec, 1.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278443, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (398.36, 169.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670184 sequences (100000146 bp) in 1.601 CPU sec, 1.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278180, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (288, 408, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (419.85, 172.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (5290, 7730, 7730)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (410, 12610)
[M::mem_pestat] mean and std.dev: (5580.18, 2680.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16303)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278217, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (284, 405, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (417.32, 173.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670948 reads in 304.876 CPU sec, 9.839 real sec
[M::mem_process_seqs] Processed 669264 reads in 188.388 CPU sec, 6.147 real sec
[M::mem_process_seqs] Processed 669660 reads in 204.686 CPU sec, 6.694 real sec
sorting block 0, #reads = 1075751 mem = 536878563 peak = 563130368
[M::process] read 670694 sequences (100000200 bp) in 2.304 CPU sec, 2.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278342, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 399, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (411.16, 173.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670682 sequences (100000026 bp) in 1.507 CPU sec, 1.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278419, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 391, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (405.21, 173.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670184 reads in 174.760 CPU sec, 5.573 real sec
[M::process] read 670956 sequences (100000093 bp) in 1.586 CPU sec, 1.586 real sec...
sorting block 1, #reads = 1075218 mem = 536881186 peak = 788193280
[M::mem_process_seqs] Processed 670694 reads in 110.320 CPU sec, 3.490 real sec
sorting block 2, #reads = 1074916 mem = 536878946 peak = 822071296
[M::process] read 669882 sequences (100000252 bp) in 2.200 CPU sec, 2.306 real sec...
[M::process] read 669338 sequences (100000073 bp) in 1.692 CPU sec, 1.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278457, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (400.28, 171.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670956 reads in 97.479 CPU sec, 3.101 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279067, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (404.05, 171.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 277159, 1, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (5735, 5735, 5822)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5561, 5996)
[M::mem_pestat] mean and std.dev: (5766.64, 41.85)
[M::mem_pestat] low and high boundaries for proper pairs: (5474, 6083)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 404, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (416.63, 174.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 670672 sequences (100000201 bp) in 1.765 CPU sec, 1.785 real sec...
[M::mem_process_seqs] Processed 670682 reads in 275.975 CPU sec, 8.827 real sec
sorting block 3, #reads = 1075374 mem = 536882994 peak = 822071296
[M::mem_process_seqs] Processed 669882 reads in 133.398 CPU sec, 4.309 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277352, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (287, 411, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (422.48, 175.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1074299 mem = 536882098 peak = 822071296
[M::process] read 670660 sequences (100000207 bp) in 3.008 CPU sec, 3.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277971, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 390, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (405.36, 173.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669338 reads in 184.220 CPU sec, 5.887 real sec
[M::process] read 670412 sequences (100000024 bp) in 1.869 CPU sec, 1.877 real sec...
[M::mem_process_seqs] Processed 670672 reads in 121.130 CPU sec, 3.846 real sec
sorting block 5, #reads = 1074431 mem = 536878821 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277457, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 392, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (406.51, 173.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670636 sequences (100000257 bp) in 2.679 CPU sec, 2.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277380, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 392, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (407.22, 172.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670512 sequences (100000281 bp) in 1.911 CPU sec, 1.930 real sec...
[M::mem_process_seqs] Processed 670660 reads in 190.661 CPU sec, 6.109 real sec
[M::mem_process_seqs] Processed 670412 reads in 122.928 CPU sec, 3.933 real sec
sorting block 6, #reads = 1075914 mem = 536877804 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278132, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 390, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (405.33, 173.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669510 sequences (100000176 bp) in 3.018 CPU sec, 3.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277960, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (402.33, 171.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (743, 1544, 1544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3146)
[M::mem_pestat] mean and std.dev: (1137.77, 529.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3947)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 670140 sequences (100000149 bp) in 1.999 CPU sec, 2.002 real sec...
[M::mem_process_seqs] Processed 670636 reads in 197.233 CPU sec, 6.310 real sec
[M::mem_process_seqs] Processed 670512 reads in 124.390 CPU sec, 3.953 real sec
sorting block 7, #reads = 1075472 mem = 536877774 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 276603, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (281, 401, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (414.79, 173.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670664 sequences (100000292 bp) in 2.872 CPU sec, 3.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277091, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (405.05, 171.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669378 sequences (100000013 bp) in 1.823 CPU sec, 1.838 real sec...
[M::mem_process_seqs] Processed 669510 reads in 197.145 CPU sec, 6.356 real sec
[M::mem_process_seqs] Processed 670140 reads in 124.674 CPU sec, 4.018 real sec
sorting block 8, #reads = 1075444 mem = 536879531 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277137, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (398.67, 170.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670254 sequences (100000219 bp) in 3.022 CPU sec, 3.339 real sec...
sorting block 9, #reads = 1074555 mem = 536878219 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277120, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 399, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (413.35, 172.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670256 sequences (100000128 bp) in 1.810 CPU sec, 1.813 real sec...
[M::mem_process_seqs] Processed 670664 reads in 198.063 CPU sec, 6.375 real sec
[M::mem_process_seqs] Processed 669378 reads in 123.884 CPU sec, 3.975 real sec
sorting block 10, #reads = 1075767 mem = 536879957 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277622, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (401.14, 169.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670358 sequences (100000218 bp) in 2.871 CPU sec, 3.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277604, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (399.95, 169.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669510 sequences (100000287 bp) in 1.731 CPU sec, 1.732 real sec...
[M::mem_process_seqs] Processed 670254 reads in 192.120 CPU sec, 6.166 real sec
[M::mem_process_seqs] Processed 670256 reads in 121.473 CPU sec, 3.866 real sec
sorting block 11, #reads = 1075288 mem = 536881935 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277894, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (398.84, 169.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669560 sequences (100000221 bp) in 3.273 CPU sec, 3.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277194, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 396, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (410.15, 171.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670358 reads in 193.080 CPU sec, 6.217 real sec
[M::mem_process_seqs] Processed 669510 reads in 124.573 CPU sec, 3.992 real sec
[M::process] read 670542 sequences (100000129 bp) in 2.353 CPU sec, 2.539 real sec...
sorting block 12, #reads = 1074659 mem = 536879566 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277254, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 392, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (406.92, 169.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670612 sequences (100000087 bp) in 3.073 CPU sec, 3.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277770, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (398.26, 169.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669560 reads in 190.014 CPU sec, 6.102 real sec
[M::mem_process_seqs] Processed 670542 reads in 122.315 CPU sec, 3.874 real sec
sorting block 13, #reads = 1075069 mem = 536876942 peak = 822071296
[M::process] read 669754 sequences (100000090 bp) in 2.307 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278053, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (397.56, 169.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1075777 mem = 536878048 peak = 822071296
[M::process] read 670386 sequences (100000118 bp) in 3.301 CPU sec, 3.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277129, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 389, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (406.00, 171.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670612 reads in 180.923 CPU sec, 5.802 real sec
[M::mem_process_seqs] Processed 669754 reads in 117.053 CPU sec, 3.716 real sec
[M::process] read 670212 sequences (100000279 bp) in 2.539 CPU sec, 2.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277572, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (403.00, 172.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1075844 mem = 536880151 peak = 822071296
[M::process] read 670280 sequences (100000114 bp) in 2.915 CPU sec, 3.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277962, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 390, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (407.10, 174.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670386 reads in 183.055 CPU sec, 5.872 real sec
[M::mem_process_seqs] Processed 670212 reads in 117.123 CPU sec, 3.707 real sec
[M::process] read 669300 sequences (100000213 bp) in 2.647 CPU sec, 2.845 real sec...
sorting block 16, #reads = 1076182 mem = 536879992 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277951, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 393, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (410.30, 175.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670762 sequences (100000135 bp) in 2.914 CPU sec, 3.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278289, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (285, 408, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (422.47, 178.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670280 reads in 181.571 CPU sec, 5.846 real sec
[M::mem_process_seqs] Processed 669300 reads in 115.553 CPU sec, 3.673 real sec
sorting block 17, #reads = 1074769 mem = 536880392 peak = 822071296
[M::process] read 670418 sequences (100000022 bp) in 2.706 CPU sec, 2.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278987, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (401.33, 172.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669844 sequences (100000002 bp) in 2.963 CPU sec, 3.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278505, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 387, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (403.40, 172.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670762 reads in 172.134 CPU sec, 5.521 real sec
[M::mem_process_seqs] Processed 670418 reads in 112.725 CPU sec, 3.571 real sec
sorting block 18, #reads = 1075699 mem = 536879174 peak = 822071296
[M::process] read 670342 sequences (100000133 bp) in 2.541 CPU sec, 2.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277628, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 401, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (415.60, 175.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1077112 mem = 536879130 peak = 822071296
[M::process] read 670230 sequences (100000036 bp) in 2.725 CPU sec, 3.009 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277792, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 395, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (409.85, 174.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669844 reads in 176.568 CPU sec, 5.674 real sec
[M::mem_process_seqs] Processed 670342 reads in 113.742 CPU sec, 3.609 real sec
[M::process] read 670104 sequences (100000025 bp) in 2.645 CPU sec, 2.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277758, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 397, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (411.72, 175.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1075627 mem = 536879882 peak = 822071296
[M::process] read 670336 sequences (100000287 bp) in 2.935 CPU sec, 3.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277859, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 399, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (413.66, 176.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670230 reads in 173.764 CPU sec, 5.581 real sec
[M::mem_process_seqs] Processed 670104 reads in 112.512 CPU sec, 3.574 real sec
[M::process] read 670428 sequences (100000091 bp) in 2.601 CPU sec, 2.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277500, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 21, #reads = 1076932 mem = 536879378 peak = 822071296
[M::mem_pestat] (25, 50, 75) percentile: (275, 392, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (408.15, 174.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669420 sequences (100000059 bp) in 2.884 CPU sec, 3.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278312, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 392, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (408.32, 176.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670336 reads in 177.623 CPU sec, 5.733 real sec
[M::mem_process_seqs] Processed 670428 reads in 115.243 CPU sec, 3.678 real sec
sorting block 22, #reads = 1076242 mem = 536880540 peak = 822071296
[M::process] read 670244 sequences (100000088 bp) in 2.843 CPU sec, 3.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278042, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (285, 405, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (418.76, 175.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278513, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 396, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (411.89, 175.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670426 sequences (100000239 bp) in 2.685 CPU sec, 3.064 real sec...
[M::mem_process_seqs] Processed 669420 reads in 171.838 CPU sec, 5.559 real sec
[M::mem_process_seqs] Processed 670244 reads in 112.460 CPU sec, 3.600 real sec
sorting block 23, #reads = 1077061 mem = 536877633 peak = 822071296
[M::process] read 670502 sequences (100000076 bp) in 2.783 CPU sec, 3.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278183, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 391, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (407.72, 174.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1076765 mem = 536879186 peak = 822071296
[M::process] read 670324 sequences (100000066 bp) in 2.611 CPU sec, 2.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278428, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 392, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (407.54, 174.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670426 reads in 175.397 CPU sec, 5.637 real sec
[M::mem_process_seqs] Processed 670502 reads in 113.679 CPU sec, 3.610 real sec
[M::process] read 670132 sequences (100000172 bp) in 3.440 CPU sec, 3.728 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278608, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 394, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (408.38, 174.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1077793 mem = 536880828 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277874, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 395, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (410.88, 176.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669698 sequences (100000197 bp) in 2.866 CPU sec, 3.127 real sec...
[M::mem_process_seqs] Processed 670324 reads in 177.646 CPU sec, 5.735 real sec
[M::mem_process_seqs] Processed 670132 reads in 115.177 CPU sec, 3.688 real sec
[M::process] read 668870 sequences (100000199 bp) in 2.619 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277678, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 396, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (411.54, 173.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1076521 mem = 536882582 peak = 822071296
[M::process] read 670204 sequences (100000258 bp) in 2.577 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277997, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 410, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (423.71, 176.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669698 reads in 173.663 CPU sec, 5.575 real sec
[M::mem_process_seqs] Processed 668870 reads in 110.912 CPU sec, 3.521 real sec
sorting block 27, #reads = 1076470 mem = 536885476 peak = 822071296
[M::process] read 670264 sequences (100000153 bp) in 2.544 CPU sec, 2.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278074, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 388, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (405.41, 173.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670082 sequences (100000081 bp) in 2.633 CPU sec, 2.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278012, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (403.19, 174.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670204 reads in 173.433 CPU sec, 5.539 real sec
[M::mem_process_seqs] Processed 670264 reads in 110.764 CPU sec, 3.507 real sec
sorting block 28, #reads = 1075088 mem = 536880046 peak = 822071296
[M::process] read 670402 sequences (100000291 bp) in 2.484 CPU sec, 2.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278302, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 395, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (411.92, 177.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1076285 mem = 536878390 peak = 822071296
[M::process] read 670382 sequences (100000113 bp) in 2.614 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278107, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 390, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (407.42, 176.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670082 reads in 178.049 CPU sec, 5.716 real sec
[M::mem_process_seqs] Processed 670402 reads in 115.064 CPU sec, 3.650 real sec
[M::process] read 670278 sequences (100000128 bp) in 2.281 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277833, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 387, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (404.79, 175.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 30, #reads = 1076849 mem = 536878128 peak = 822071296
[M::process] read 669422 sequences (100000133 bp) in 3.051 CPU sec, 3.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278042, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (403.01, 173.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670382 reads in 176.196 CPU sec, 5.637 real sec
[M::mem_process_seqs] Processed 670278 reads in 113.137 CPU sec, 3.583 real sec
[M::process] read 670294 sequences (100000177 bp) in 2.180 CPU sec, 2.285 real sec...
sorting block 31, #reads = 1075365 mem = 536879266 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277775, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 400, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (415.90, 175.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670598 sequences (100000244 bp) in 2.822 CPU sec, 3.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278780, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (403.17, 173.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669422 reads in 175.468 CPU sec, 5.648 real sec
[M::mem_process_seqs] Processed 670294 reads in 115.823 CPU sec, 3.674 real sec
sorting block 32, #reads = 1075729 mem = 536876531 peak = 822071296
[M::process] read 670518 sequences (100000261 bp) in 2.730 CPU sec, 2.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278450, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 385, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (403.27, 174.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670454 sequences (100000079 bp) in 2.538 CPU sec, 2.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278064, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (402.79, 174.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670598 reads in 177.719 CPU sec, 5.698 real sec
[M::mem_process_seqs] Processed 670518 reads in 115.585 CPU sec, 3.666 real sec
sorting block 33, #reads = 1076024 mem = 536877332 peak = 822071296
[M::process] read 670470 sequences (100000047 bp) in 2.212 CPU sec, 2.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277965, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (402.27, 174.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670372 sequences (100000146 bp) in 2.932 CPU sec, 3.172 real sec...
sorting block 34, #reads = 1075266 mem = 536877544 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278645, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (405.26, 175.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670454 reads in 173.411 CPU sec, 5.575 real sec
[M::mem_process_seqs] Processed 670470 reads in 112.458 CPU sec, 3.562 real sec
[M::process] read 670356 sequences (100000190 bp) in 2.638 CPU sec, 2.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278369, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 392, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (409.34, 178.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1075133 mem = 536886376 peak = 822071296
[M::process] read 670938 sequences (100000050 bp) in 2.700 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278336, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 390, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (408.88, 178.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670372 reads in 174.478 CPU sec, 5.600 real sec
[M::mem_process_seqs] Processed 670356 reads in 113.673 CPU sec, 3.603 real sec
[M::process] read 670810 sequences (100000182 bp) in 2.562 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279107, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 36, #reads = 1074709 mem = 536881282 peak = 822071296
[M::mem_pestat] (25, 50, 75) percentile: (267, 381, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (396.90, 169.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671124 sequences (100000282 bp) in 3.117 CPU sec, 3.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278804, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 378, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (392.97, 164.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670938 reads in 181.128 CPU sec, 5.853 real sec
[M::mem_process_seqs] Processed 670810 reads in 117.260 CPU sec, 3.747 real sec
sorting block 37, #reads = 1074603 mem = 536877287 peak = 822071296
[M::process] read 670718 sequences (100000105 bp) in 2.706 CPU sec, 2.926 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278885, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 380, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (393.23, 165.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670460 sequences (100000285 bp) in 2.853 CPU sec, 3.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278652, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 382, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (395.42, 165.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671124 reads in 180.612 CPU sec, 5.808 real sec
[M::mem_process_seqs] Processed 670718 reads in 116.646 CPU sec, 3.713 real sec
sorting block 38, #reads = 1073723 mem = 536879661 peak = 822071296
[M::process] read 670306 sequences (100000100 bp) in 2.778 CPU sec, 2.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278225, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 383, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (395.96, 164.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278046, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 393, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (406.14, 169.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1075029 mem = 536877453 peak = 822071296
[M::process] read 669184 sequences (100000234 bp) in 3.232 CPU sec, 3.598 real sec...
[M::mem_process_seqs] Processed 670460 reads in 176.590 CPU sec, 5.695 real sec
[M::mem_process_seqs] Processed 670306 reads in 115.506 CPU sec, 3.691 real sec
[M::process] read 670058 sequences (100000240 bp) in 3.039 CPU sec, 3.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277634, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (292, 411, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (421.29, 171.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1074481 mem = 536879778 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278175, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 392, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (406.41, 169.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670738 sequences (100000289 bp) in 2.901 CPU sec, 3.108 real sec...
[M::mem_process_seqs] Processed 669184 reads in 179.179 CPU sec, 5.805 real sec
[M::mem_process_seqs] Processed 670058 reads in 116.510 CPU sec, 3.738 real sec
sorting block 41, #reads = 1074376 mem = 536878425 peak = 822071296
[M::process] read 670636 sequences (100000105 bp) in 2.870 CPU sec, 3.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278364, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (398.73, 168.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278519, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (402.45, 170.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670494 sequences (100000098 bp) in 2.813 CPU sec, 3.047 real sec...
[M::mem_process_seqs] Processed 670738 reads in 179.410 CPU sec, 5.764 real sec
[M::mem_process_seqs] Processed 670636 reads in 117.721 CPU sec, 3.749 real sec
sorting block 42, #reads = 1074716 mem = 536879062 peak = 822071296
[M::process] read 670536 sequences (100000168 bp) in 2.755 CPU sec, 2.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278188, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 387, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (401.92, 169.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (5484, 5663, 5663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5126, 6021)
[M::mem_pestat] mean and std.dev: (5677.00, 119.98)
[M::mem_pestat] low and high boundaries for proper pairs: (4947, 6200)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 277627, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (400.02, 168.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670572 sequences (100000212 bp) in 3.030 CPU sec, 3.236 real sec...
[M::mem_process_seqs] Processed 670494 reads in 184.949 CPU sec, 5.934 real sec
[M::mem_process_seqs] Processed 670536 reads in 119.099 CPU sec, 3.781 real sec
sorting block 43, #reads = 1076371 mem = 536877429 peak = 822071296
[M::process] read 670290 sequences (100000263 bp) in 2.881 CPU sec, 3.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277798, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (402.50, 170.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1075230 mem = 536880640 peak = 822071296
[M::process] read 669920 sequences (100000070 bp) in 2.900 CPU sec, 3.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277750, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 391, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (406.24, 172.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670572 reads in 182.789 CPU sec, 5.862 real sec
[M::mem_process_seqs] Processed 670290 reads in 118.157 CPU sec, 3.753 real sec
[M::process] read 669076 sequences (100000188 bp) in 2.527 CPU sec, 2.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277720, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 396, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (409.48, 170.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1074644 mem = 536881400 peak = 822071296
[M::process] read 670158 sequences (100000290 bp) in 2.996 CPU sec, 3.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277201, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (285, 402, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (414.52, 169.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669920 reads in 185.396 CPU sec, 5.940 real sec
[M::mem_process_seqs] Processed 669076 reads in 119.561 CPU sec, 3.791 real sec
[M::process] read 670240 sequences (100000285 bp) in 2.516 CPU sec, 2.686 real sec...
sorting block 46, #reads = 1075243 mem = 536878696 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277575, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 390, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (405.41, 170.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670250 sequences (100000099 bp) in 3.216 CPU sec, 3.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277174, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 389, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (404.41, 171.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670158 reads in 185.764 CPU sec, 5.949 real sec
[M::mem_process_seqs] Processed 670240 reads in 121.918 CPU sec, 3.874 real sec
[M::process] read 670360 sequences (100000119 bp) in 2.392 CPU sec, 2.545 real sec...
sorting block 47, #reads = 1075208 mem = 536878804 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277568, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (404.90, 171.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670344 sequences (100000205 bp) in 3.189 CPU sec, 3.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277148, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 389, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (404.45, 172.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670250 reads in 189.950 CPU sec, 6.081 real sec
[M::mem_process_seqs] Processed 670360 reads in 118.639 CPU sec, 3.767 real sec
sorting block 48, #reads = 1075085 mem = 536879250 peak = 822071296
[M::process] read 670454 sequences (100000125 bp) in 1.981 CPU sec, 2.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 276893, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (403.87, 172.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1075685 mem = 536880001 peak = 822071296
[M::process] read 670328 sequences (100000087 bp) in 2.980 CPU sec, 3.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277157, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (398.11, 169.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670344 reads in 194.687 CPU sec, 6.253 real sec
[M::process] read 670510 sequences (100000260 bp) in 2.069 CPU sec, 2.149 real sec...
[M::process] read 670544 sequences (100000096 bp) in 2.177 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 276818, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (400.85, 172.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670510 reads in 100.868 CPU sec, 3.282 real sec
sorting block 50, #reads = 1076234 mem = 536882068 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277170, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 385, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (402.47, 173.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670328 reads in 205.049 CPU sec, 7.333 real sec
[M::process] read 670380 sequences (100000235 bp) in 2.567 CPU sec, 2.775 real sec...
[M::mem_process_seqs] Processed 670454 reads in 401.079 CPU sec, 13.606 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277168, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (400.57, 172.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1075094 mem = 536880789 peak = 822071296
[M::process] read 670342 sequences (100000100 bp) in 2.728 CPU sec, 2.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 276542, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (400.69, 171.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670544 reads in 208.626 CPU sec, 6.705 real sec
[M::mem_process_seqs] Processed 670380 reads in 122.063 CPU sec, 3.876 real sec
sorting block 52, #reads = 1076486 mem = 536880354 peak = 822071296
[M::process] read 670496 sequences (100000018 bp) in 2.751 CPU sec, 2.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277735, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (404.36, 173.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670356 sequences (100000208 bp) in 2.710 CPU sec, 2.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278331, 10, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 385, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (403.85, 175.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1063, 1723, 1723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3043)
[M::mem_pestat] mean and std.dev: (1382.40, 367.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3703)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 670342 reads in 180.745 CPU sec, 5.822 real sec
[M::mem_process_seqs] Processed 670496 reads in 112.541 CPU sec, 3.574 real sec
sorting block 53, #reads = 1074549 mem = 536879144 peak = 822071296
[M::process] read 670494 sequences (100000176 bp) in 2.585 CPU sec, 2.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277915, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 389, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (406.85, 176.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1076016 mem = 536878330 peak = 822071296
[M::process] read 670732 sequences (100000297 bp) in 2.848 CPU sec, 3.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278108, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 389, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (408.89, 180.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670356 reads in 187.140 CPU sec, 6.002 real sec
[M::mem_process_seqs] Processed 670494 reads in 119.413 CPU sec, 3.786 real sec
[M::process] read 670060 sequences (100000083 bp) in 2.318 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277179, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (396.52, 166.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1075567 mem = 536878793 peak = 822071296
[M::process] read 669858 sequences (100000163 bp) in 3.144 CPU sec, 3.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 276512, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 386, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (399.94, 164.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1448, 5495, 5495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13589)
[M::mem_pestat] mean and std.dev: (4019.50, 2309.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17636)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 670732 reads in 182.210 CPU sec, 5.829 real sec
[M::mem_process_seqs] Processed 670060 reads in 116.330 CPU sec, 3.701 real sec
[M::process] read 669894 sequences (100000206 bp) in 2.307 CPU sec, 2.410 real sec...
sorting block 56, #reads = 1073975 mem = 536879013 peak = 822071296
[M::process] read 670354 sequences (100000203 bp) in 3.264 CPU sec, 3.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 276297, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 390, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (403.79, 167.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669894 reads in 104.644 CPU sec, 3.313 real sec
sorting block 57, #reads = 1074819 mem = 536879719 peak = 822071296
[M::process] read 670154 sequences (100000126 bp) in 3.024 CPU sec, 3.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 276994, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 277297, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 381, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (395.59, 164.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] (25, 50, 75) percentile: (277, 390, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (403.44, 166.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669372 sequences (100000128 bp) in 1.593 CPU sec, 1.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277283, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 382, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (397.23, 165.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670354 reads in 192.437 CPU sec, 6.121 real sec
[M::mem_process_seqs] Processed 669858 reads in 374.038 CPU sec, 11.975 real sec
[M::mem_process_seqs] Processed 670154 reads in 125.536 CPU sec, 4.064 real sec
sorting block 58, #reads = 1076138 mem = 536880013 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 277457, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (5604, 5604, 5604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5604, 5604)
[M::mem_pestat] mean and std.dev: (5604.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (5604, 5604)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (284, 399, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (411.76, 168.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1196, 5410, 5410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13838)
[M::mem_pestat] mean and std.dev: (3499.77, 2136.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18052)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 669372 reads in 97.916 CPU sec, 3.317 real sec
[M::process] read 669106 sequences (100000284 bp) in 3.094 CPU sec, 3.398 real sec...
sorting block 59, #reads = 1076072 mem = 536879578 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 276751, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (291, 407, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (419.26, 170.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670586 sequences (100000086 bp) in 2.872 CPU sec, 3.162 real sec...
[M::process] read 670476 sequences (100000133 bp) in 2.524 CPU sec, 2.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277512, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 387, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (401.47, 169.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670586 reads in 105.422 CPU sec, 3.419 real sec
sorting block 60, #reads = 1075684 mem = 536878936 peak = 822071296
[M::process] read 670816 sequences (100000162 bp) in 2.754 CPU sec, 2.970 real sec...
[M::mem_process_seqs] Processed 669106 reads in 279.959 CPU sec, 9.109 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277061, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 389, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (402.11, 168.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277609, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (395.78, 166.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670704 sequences (100000262 bp) in 3.028 CPU sec, 3.318 real sec...
[M::mem_process_seqs] Processed 670476 reads in 208.409 CPU sec, 6.631 real sec
sorting block 61, #reads = 1074497 mem = 536878062 peak = 822071296
[M::mem_process_seqs] Processed 670816 reads in 122.124 CPU sec, 3.880 real sec
[M::process] read 670892 sequences (100000216 bp) in 2.983 CPU sec, 3.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277861, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (395.53, 166.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 62, #reads = 1075244 mem = 536877414 peak = 822071296
[M::process] read 670662 sequences (100000190 bp) in 2.567 CPU sec, 2.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277352, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (399.38, 168.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670704 reads in 188.672 CPU sec, 6.039 real sec
[M::mem_process_seqs] Processed 670892 reads in 120.915 CPU sec, 3.828 real sec
[M::process] read 670782 sequences (100000297 bp) in 2.553 CPU sec, 2.728 real sec...
sorting block 63, #reads = 1075461 mem = 536877026 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277017, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (399.40, 168.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669564 sequences (100000069 bp) in 2.794 CPU sec, 3.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 276626, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (400.14, 169.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670662 reads in 188.381 CPU sec, 6.010 real sec
[M::process] read 669900 sequences (100000042 bp) in 1.776 CPU sec, 1.814 real sec...
[M::mem_process_seqs] Processed 670782 reads in 123.634 CPU sec, 3.931 real sec
sorting block 64, #reads = 1075867 mem = 536878671 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 277002, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 400, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (413.06, 171.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670560 sequences (100000294 bp) in 3.204 CPU sec, 3.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 276545, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 395, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (408.41, 169.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670664 sequences (100000057 bp) in 1.919 CPU sec, 1.948 real sec...
[M::mem_process_seqs] Processed 669564 reads in 192.417 CPU sec, 6.131 real sec
[M::mem_process_seqs] Processed 669900 reads in 121.489 CPU sec, 3.848 real sec
sorting block 65, #reads = 1074790 mem = 536887580 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277141, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (401.57, 169.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 66, #reads = 1076542 mem = 536879769 peak = 822071296
[M::process] read 669724 sequences (100000074 bp) in 2.973 CPU sec, 3.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277494, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (401.84, 169.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670178 sequences (100000078 bp) in 2.096 CPU sec, 2.097 real sec...
[M::mem_process_seqs] Processed 670560 reads in 194.527 CPU sec, 6.194 real sec
[M::mem_process_seqs] Processed 670664 reads in 123.412 CPU sec, 3.898 real sec
sorting block 67, #reads = 1075669 mem = 536876602 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 276467, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 397, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (409.99, 170.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670378 sequences (100000215 bp) in 3.204 CPU sec, 3.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 276837, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 392, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (406.41, 170.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670480 sequences (100000145 bp) in 2.084 CPU sec, 2.104 real sec...
[M::mem_process_seqs] Processed 669724 reads in 196.324 CPU sec, 6.242 real sec
[M::mem_process_seqs] Processed 670178 reads in 122.127 CPU sec, 3.873 real sec
sorting block 68, #reads = 1076069 mem = 536878293 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 276120, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (400.63, 168.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670822 sequences (100000225 bp) in 3.252 CPU sec, 3.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277182, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (399.13, 168.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670378 reads in 196.591 CPU sec, 6.293 real sec
[M::process] read 670812 sequences (100000274 bp) in 2.111 CPU sec, 2.119 real sec...
[M::mem_process_seqs] Processed 670480 reads in 125.505 CPU sec, 4.007 real sec
sorting block 69, #reads = 1075935 mem = 536880193 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277490, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (396.82, 168.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670754 sequences (100000082 bp) in 3.305 CPU sec, 3.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277409, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (395.35, 167.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670822 reads in 195.664 CPU sec, 6.250 real sec
[M::process] read 670612 sequences (100000244 bp) in 2.233 CPU sec, 2.271 real sec...
[M::mem_process_seqs] Processed 670812 reads in 125.459 CPU sec, 3.972 real sec
sorting block 70, #reads = 1074543 mem = 536880503 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 277562, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 219, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (239.44, 44.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (397.17, 169.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 71, #reads = 1075300 mem = 536882152 peak = 822071296
[M::process] read 670618 sequences (100000094 bp) in 3.348 CPU sec, 3.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277792, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (399.47, 170.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670754 reads in 196.881 CPU sec, 6.294 real sec
[M::mem_process_seqs] Processed 670612 reads in 127.359 CPU sec, 4.027 real sec
[M::process] read 670422 sequences (100000151 bp) in 2.443 CPU sec, 2.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277589, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (401.02, 171.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 72, #reads = 1073469 mem = 536884802 peak = 822071296
[M::process] read 670724 sequences (100000082 bp) in 3.255 CPU sec, 3.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277683, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 385, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (402.27, 172.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670618 reads in 191.978 CPU sec, 6.155 real sec
[M::mem_process_seqs] Processed 670422 reads in 124.565 CPU sec, 3.959 real sec
[M::process] read 672344 sequences (100000033 bp) in 2.212 CPU sec, 2.333 real sec...
sorting block 73, #reads = 1074078 mem = 536880247 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278426, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 381, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (400.06, 174.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (5437, 5437, 5437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5437, 5437)
[M::mem_pestat] mean and std.dev: (5437.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (5437, 5437)
[M::mem_pestat] skip orientation RR
[M::process] read 670788 sequences (100000231 bp) in 3.718 CPU sec, 4.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279076, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 371, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (391.50, 175.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670724 reads in 194.788 CPU sec, 6.277 real sec
[M::mem_process_seqs] Processed 672344 reads in 128.661 CPU sec, 4.102 real sec
sorting block 74, #reads = 1074347 mem = 536880894 peak = 822071296
[M::process] read 670628 sequences (100000097 bp) in 2.897 CPU sec, 3.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278729, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 402, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082)
[M::mem_pestat] mean and std.dev: (415.29, 180.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1352)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671828 sequences (100000016 bp) in 3.064 CPU sec, 3.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277834, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 402, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073)
[M::mem_pestat] mean and std.dev: (413.43, 177.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (597, 597, 1101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2109)
[M::mem_pestat] mean and std.dev: (639.89, 201.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2613)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 670788 reads in 198.095 CPU sec, 6.335 real sec
[M::mem_process_seqs] Processed 670628 reads in 126.119 CPU sec, 3.988 real sec
sorting block 75, #reads = 1071937 mem = 536883488 peak = 822071296
[M::process] read 671734 sequences (100000177 bp) in 2.490 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278480, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (396.45, 173.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 76, #reads = 1070443 mem = 536877618 peak = 822071296
[M::process] read 671982 sequences (100000258 bp) in 3.094 CPU sec, 3.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278488, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 383, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (398.86, 174.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671828 reads in 196.509 CPU sec, 6.285 real sec
[M::mem_process_seqs] Processed 671734 reads in 126.237 CPU sec, 3.997 real sec
[M::process] read 670974 sequences (100000148 bp) in 2.391 CPU sec, 2.470 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279129, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 376, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (392.86, 172.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 77, #reads = 1070815 mem = 536880293 peak = 822071296
[M::process] read 670408 sequences (100000056 bp) in 2.964 CPU sec, 3.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278739, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 393, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (406.53, 174.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671982 reads in 197.144 CPU sec, 6.334 real sec
[M::process] read 672174 sequences (100000177 bp) in 1.705 CPU sec, 1.746 real sec...
[M::mem_process_seqs] Processed 670974 reads in 129.224 CPU sec, 4.115 real sec
sorting block 78, #reads = 1071940 mem = 536878439 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278038, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 401, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (411.49, 173.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672170 sequences (100000030 bp) in 3.037 CPU sec, 3.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278384, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 377, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (393.39, 171.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670408 reads in 197.441 CPU sec, 6.327 real sec
[M::process] read 671616 sequences (100000213 bp) in 2.101 CPU sec, 2.141 real sec...
[M::mem_process_seqs] Processed 672174 reads in 129.429 CPU sec, 4.098 real sec
sorting block 79, #reads = 1070477 mem = 536879651 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279409, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 372, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (387.62, 167.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671220 sequences (100000102 bp) in 3.165 CPU sec, 3.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278642, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 384, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (397.63, 171.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672170 reads in 200.656 CPU sec, 6.425 real sec
[M::process] read 669936 sequences (100000119 bp) in 2.171 CPU sec, 2.180 real sec...
sorting block 80, #reads = 1070433 mem = 536878696 peak = 822071296
[M::mem_process_seqs] Processed 671616 reads in 127.462 CPU sec, 4.055 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278415, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 384, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (399.00, 171.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 81, #reads = 1070928 mem = 536876938 peak = 822071296
[M::process] read 670380 sequences (100000289 bp) in 3.145 CPU sec, 3.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278424, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 406, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (417.00, 174.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671222 sequences (100000059 bp) in 2.264 CPU sec, 2.275 real sec...
[M::mem_process_seqs] Processed 671220 reads in 206.287 CPU sec, 6.625 real sec
[M::mem_process_seqs] Processed 669936 reads in 130.141 CPU sec, 4.136 real sec
sorting block 82, #reads = 1071240 mem = 536880221 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279050, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 398, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (410.11, 172.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669868 sequences (100000055 bp) in 3.212 CPU sec, 3.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278653, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (399.93, 170.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670836 sequences (100000091 bp) in 2.119 CPU sec, 2.127 real sec...
[M::mem_process_seqs] Processed 670380 reads in 202.314 CPU sec, 6.457 real sec
[M::mem_process_seqs] Processed 671222 reads in 129.376 CPU sec, 4.128 real sec
sorting block 83, #reads = 1070844 mem = 536879273 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277715, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 400, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (411.53, 172.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670494 sequences (100000282 bp) in 3.531 CPU sec, 3.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278685, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (396.45, 166.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669868 reads in 204.533 CPU sec, 6.585 real sec
[M::process] read 670368 sequences (100000073 bp) in 2.287 CPU sec, 2.326 real sec...
[M::mem_process_seqs] Processed 670836 reads in 130.736 CPU sec, 4.215 real sec
sorting block 84, #reads = 1071346 mem = 536877677 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278556, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 388, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (400.67, 168.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669676 sequences (100000082 bp) in 3.079 CPU sec, 3.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278586, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 393, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (405.51, 170.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670494 reads in 202.616 CPU sec, 6.495 real sec
[M::process] read 671046 sequences (100000127 bp) in 2.349 CPU sec, 2.375 real sec...
sorting block 85, #reads = 1071871 mem = 536882179 peak = 822071296
[M::mem_process_seqs] Processed 670368 reads in 128.986 CPU sec, 4.074 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278459, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 406, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (414.94, 171.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 86, #reads = 1071281 mem = 536881514 peak = 822071296
[M::process] read 670838 sequences (100000211 bp) in 3.167 CPU sec, 3.603 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278450, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (396.11, 168.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669676 reads in 206.457 CPU sec, 6.633 real sec
[M::process] read 670138 sequences (100000219 bp) in 2.328 CPU sec, 2.349 real sec...
[M::mem_process_seqs] Processed 671046 reads in 132.651 CPU sec, 4.212 real sec
sorting block 87, #reads = 1070206 mem = 536879948 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278987, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (396.60, 168.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670584 sequences (100000247 bp) in 3.279 CPU sec, 3.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278222, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 397, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (409.63, 172.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670838 reads in 204.404 CPU sec, 6.570 real sec
[M::process] read 670822 sequences (100000100 bp) in 2.029 CPU sec, 2.070 real sec...
[M::mem_process_seqs] Processed 670138 reads in 131.116 CPU sec, 4.176 real sec
sorting block 88, #reads = 1070874 mem = 536879142 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278754, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 388, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (403.36, 172.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670788 sequences (100000225 bp) in 3.250 CPU sec, 3.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278430, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 389, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (404.33, 173.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670584 reads in 199.990 CPU sec, 6.403 real sec
[M::process] read 671260 sequences (100000003 bp) in 2.365 CPU sec, 2.400 real sec...
[M::mem_process_seqs] Processed 670822 reads in 128.405 CPU sec, 4.067 real sec
sorting block 89, #reads = 1070552 mem = 536877338 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278486, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 391, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (407.64, 176.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673862 sequences (100000200 bp) in 2.844 CPU sec, 3.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279363, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 389, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (404.69, 175.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672624 sequences (100000265 bp) in 1.986 CPU sec, 2.007 real sec...
[M::mem_process_seqs] Processed 670788 reads in 205.436 CPU sec, 6.598 real sec
sorting block 90, #reads = 1071829 mem = 536877813 peak = 822071296
[M::mem_process_seqs] Processed 671260 reads in 132.613 CPU sec, 4.225 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280294, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 356, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (372.13, 162.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 91, #reads = 1070526 mem = 536882435 peak = 822071296
[M::process] read 672540 sequences (100000204 bp) in 3.376 CPU sec, 3.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280347, 6, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 371, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (383.09, 163.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (4506, 4506, 6823)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11457)
[M::mem_pestat] mean and std.dev: (4271.23, 2563.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14524)
[M::mem_pestat] skip orientation RR
[M::process] read 672424 sequences (100000096 bp) in 2.265 CPU sec, 2.266 real sec...
[M::mem_process_seqs] Processed 673862 reads in 222.907 CPU sec, 7.158 real sec
[M::mem_process_seqs] Processed 672624 reads in 141.418 CPU sec, 4.500 real sec
sorting block 92, #reads = 1064778 mem = 536878051 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279413, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 366, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (379.85, 163.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672510 sequences (100000061 bp) in 3.740 CPU sec, 4.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 279879, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (1367, 2654, 2654)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5228)
[M::mem_pestat] mean and std.dev: (1816.83, 922.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 370, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (383.82, 164.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 672540 reads in 214.597 CPU sec, 6.876 real sec
[M::mem_process_seqs] Processed 672424 reads in 133.819 CPU sec, 4.242 real sec
[M::process] read 671480 sequences (100000027 bp) in 2.526 CPU sec, 2.654 real sec...
sorting block 93, #reads = 1064412 mem = 536878247 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280005, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 364, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (379.75, 163.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671974 sequences (100000106 bp) in 3.138 CPU sec, 3.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279607, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (393.24, 167.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672510 reads in 205.545 CPU sec, 6.636 real sec
[M::mem_process_seqs] Processed 671480 reads in 130.422 CPU sec, 4.150 real sec
[M::process] read 672192 sequences (100000196 bp) in 2.434 CPU sec, 2.491 real sec...
sorting block 94, #reads = 1067650 mem = 536877828 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279508, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (388.10, 166.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672374 sequences (100000155 bp) in 3.209 CPU sec, 3.723 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280035, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 370, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (385.02, 166.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671974 reads in 204.566 CPU sec, 6.604 real sec
sorting block 95, #reads = 1068249 mem = 536878895 peak = 822071296
[M::mem_process_seqs] Processed 672192 reads in 133.653 CPU sec, 4.260 real sec
[M::process] read 671796 sequences (100000145 bp) in 3.100 CPU sec, 3.418 real sec...
sorting block 96, #reads = 1069927 mem = 536878001 peak = 822071296
[M::process] read 670768 sequences (100000188 bp) in 3.182 CPU sec, 3.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279834, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 376, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (391.95, 170.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671796 reads in 115.639 CPU sec, 3.677 real sec
[M::process] read 670330 sequences (100000160 bp) in 2.376 CPU sec, 2.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280094, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 370, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (385.21, 166.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279225, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 392, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (403.79, 171.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672374 reads in 317.899 CPU sec, 10.259 real sec
[M::mem_process_seqs] Processed 670768 reads in 150.473 CPU sec, 4.840 real sec
sorting block 97, #reads = 1069360 mem = 536878281 peak = 822071296
[M::process] read 671228 sequences (100000093 bp) in 2.906 CPU sec, 3.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279234, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 403, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (414.41, 175.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 98, #reads = 1070128 mem = 536877958 peak = 822071296
[M::process] read 670496 sequences (100000078 bp) in 3.559 CPU sec, 3.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279594, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (400.02, 172.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670330 reads in 204.952 CPU sec, 6.631 real sec
[M::mem_process_seqs] Processed 671228 reads in 132.730 CPU sec, 4.245 real sec
[M::process] read 670852 sequences (100000298 bp) in 3.209 CPU sec, 3.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278579, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 395, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (410.27, 177.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 99, #reads = 1069320 mem = 536879493 peak = 822071296
[M::process] read 670598 sequences (100000261 bp) in 2.937 CPU sec, 3.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278923, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 390, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (403.79, 173.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670496 reads in 206.749 CPU sec, 6.737 real sec
[M::mem_process_seqs] Processed 670852 reads in 133.995 CPU sec, 4.347 real sec
sorting block 100, #reads = 1069888 mem = 536879727 peak = 822071296
[M::process] read 670362 sequences (100000229 bp) in 3.058 CPU sec, 3.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278694, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 392, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (406.34, 174.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669550 sequences (100000057 bp) in 2.744 CPU sec, 3.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278769, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 395, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (409.68, 175.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670598 reads in 205.062 CPU sec, 6.644 real sec
[M::mem_process_seqs] Processed 670362 reads in 133.084 CPU sec, 4.420 real sec
sorting block 101, #reads = 1071130 mem = 536880381 peak = 822071296
[M::process] read 670604 sequences (100000082 bp) in 3.142 CPU sec, 3.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278519, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 409, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (421.88, 177.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670802 sequences (100000111 bp) in 2.976 CPU sec, 3.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278763, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 392, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (407.49, 175.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669550 reads in 201.273 CPU sec, 6.537 real sec
[M::mem_process_seqs] Processed 670604 reads in 132.279 CPU sec, 4.234 real sec
sorting block 102, #reads = 1071402 mem = 536878895 peak = 822071296
[M::process] read 670536 sequences (100000107 bp) in 2.606 CPU sec, 2.835 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278621, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 388, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (404.44, 175.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 103, #reads = 1072439 mem = 536879202 peak = 822071296
[M::process] read 670598 sequences (100000027 bp) in 3.179 CPU sec, 3.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278953, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 394, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (410.10, 178.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1325)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670802 reads in 203.669 CPU sec, 6.568 real sec
[M::mem_process_seqs] Processed 670536 reads in 131.865 CPU sec, 4.218 real sec
[M::process] read 670770 sequences (100000058 bp) in 2.336 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278933, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 396, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (412.29, 180.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1334)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 104, #reads = 1071348 mem = 536879265 peak = 822071296
[M::process] read 670710 sequences (100000183 bp) in 3.270 CPU sec, 3.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278970, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 393, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (411.38, 182.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1343)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670598 reads in 202.318 CPU sec, 6.534 real sec
[M::mem_process_seqs] Processed 670770 reads in 131.148 CPU sec, 4.185 real sec
[M::process] read 672014 sequences (100000125 bp) in 2.433 CPU sec, 2.550 real sec...
sorting block 105, #reads = 1071568 mem = 536880553 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278894, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 393, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (410.30, 180.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1332)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671942 sequences (100000077 bp) in 3.203 CPU sec, 3.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278886, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 374, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (390.75, 170.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671620 sequences (100000288 bp) in 2.003 CPU sec, 2.012 real sec...
[M::mem_process_seqs] Processed 670710 reads in 225.676 CPU sec, 7.238 real sec
[M::mem_process_seqs] Processed 672014 reads in 150.599 CPU sec, 4.797 real sec
sorting block 106, #reads = 1069669 mem = 536880283 peak = 822071296
[M::process] read 671462 sequences (100000004 bp) in 3.200 CPU sec, 3.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278892, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (392.42, 168.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671620 reads in 109.121 CPU sec, 3.512 real sec
sorting block 107, #reads = 1063362 mem = 536880777 peak = 822071296
[M::process] read 671332 sequences (100000051 bp) in 2.084 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279184, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (389.52, 166.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278638, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (393.27, 167.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671942 reads in 303.918 CPU sec, 9.813 real sec
[M::process] read 670876 sequences (100000202 bp) in 2.234 CPU sec, 2.364 real sec...
[M::mem_process_seqs] Processed 671462 reads in 142.462 CPU sec, 4.598 real sec
sorting block 108, #reads = 1071850 mem = 536880138 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278250, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (394.92, 168.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669936 sequences (100000079 bp) in 3.354 CPU sec, 4.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278464, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 390, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (401.20, 169.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671332 reads in 195.004 CPU sec, 6.314 real sec
[M::mem_process_seqs] Processed 670876 reads in 122.828 CPU sec, 3.939 real sec
sorting block 109, #reads = 1071990 mem = 536879955 peak = 822071296
[M::process] read 671810 sequences (100000241 bp) in 2.632 CPU sec, 2.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278551, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 400, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (409.97, 167.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 110, #reads = 1072405 mem = 536878323 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279606, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (389.20, 165.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671952 sequences (100000147 bp) in 3.475 CPU sec, 3.999 real sec...
[M::mem_process_seqs] Processed 669936 reads in 197.702 CPU sec, 6.436 real sec
[M::mem_process_seqs] Processed 671810 reads in 130.115 CPU sec, 4.165 real sec
[M::process] read 672314 sequences (100000214 bp) in 2.894 CPU sec, 3.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278983, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 367, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (381.78, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 111, #reads = 1071024 mem = 536877984 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278965, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 366, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (380.27, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672060 sequences (100000167 bp) in 3.233 CPU sec, 3.616 real sec...
[M::mem_process_seqs] Processed 671952 reads in 203.169 CPU sec, 6.606 real sec
[M::mem_process_seqs] Processed 672314 reads in 131.849 CPU sec, 4.214 real sec
[M::process] read 671600 sequences (100000101 bp) in 3.070 CPU sec, 3.462 real sec...
sorting block 112, #reads = 1073126 mem = 536880566 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279305, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 368, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (382.53, 162.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671610 sequences (100000025 bp) in 2.923 CPU sec, 3.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278988, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 375, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (389.05, 166.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672060 reads in 198.126 CPU sec, 6.395 real sec
[M::mem_process_seqs] Processed 671600 reads in 121.586 CPU sec, 3.868 real sec
sorting block 113, #reads = 1070616 mem = 536882837 peak = 822071296
[M::process] read 670858 sequences (100000184 bp) in 2.695 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278718, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (391.17, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670662 sequences (100000170 bp) in 2.802 CPU sec, 3.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278435, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 389, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (401.07, 168.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671610 reads in 192.841 CPU sec, 6.144 real sec
[M::mem_process_seqs] Processed 670858 reads in 123.912 CPU sec, 3.916 real sec
sorting block 114, #reads = 1072626 mem = 536889458 peak = 822071296
[M::process] read 671556 sequences (100000069 bp) in 2.481 CPU sec, 2.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279141, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 389, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (401.08, 167.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 115, #reads = 1072374 mem = 536877976 peak = 822071296
[M::process] read 671480 sequences (100000219 bp) in 2.868 CPU sec, 3.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278644, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (390.21, 165.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670662 reads in 192.934 CPU sec, 6.167 real sec
[M::process] read 671298 sequences (100000247 bp) in 2.285 CPU sec, 2.340 real sec...
[M::mem_process_seqs] Processed 671556 reads in 124.779 CPU sec, 3.951 real sec
sorting block 116, #reads = 1073339 mem = 536879082 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279191, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (392.12, 166.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671148 sequences (100000154 bp) in 2.951 CPU sec, 3.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278810, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (393.24, 166.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671030 sequences (100000197 bp) in 2.023 CPU sec, 2.031 real sec...
[M::mem_process_seqs] Processed 671480 reads in 198.352 CPU sec, 6.329 real sec
[M::mem_process_seqs] Processed 671298 reads in 124.840 CPU sec, 3.958 real sec
sorting block 117, #reads = 1072073 mem = 536878281 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278295, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (397.84, 168.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671096 sequences (100000097 bp) in 2.450 CPU sec, 2.680 real sec...
[M::process] read 671186 sequences (100000043 bp) in 1.630 CPU sec, 1.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278646, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 386, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (399.11, 168.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671148 reads in 186.050 CPU sec, 5.930 real sec
sorting block 118, #reads = 1071792 mem = 536880458 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278576, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (399.89, 170.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671224 sequences (100000119 bp) in 2.434 CPU sec, 2.443 real sec...
[M::mem_process_seqs] Processed 671096 reads in 108.574 CPU sec, 3.433 real sec
[M::mem_process_seqs] Processed 671030 reads in 271.974 CPU sec, 8.660 real sec
sorting block 119, #reads = 1070174 mem = 536878857 peak = 822071296
[M::process] read 671030 sequences (100000198 bp) in 2.717 CPU sec, 2.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278695, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 385, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (400.06, 172.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671224 reads in 113.220 CPU sec, 3.566 real sec
[M::process] read 671402 sequences (100000187 bp) in 2.283 CPU sec, 2.303 real sec...
sorting block 120, #reads = 1070879 mem = 536879527 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278998, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 388, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (401.77, 173.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278698, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 390, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (403.22, 173.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671186 reads in 330.777 CPU sec, 10.509 real sec
[M::process] read 671618 sequences (100000186 bp) in 2.733 CPU sec, 2.784 real sec...
[M::mem_process_seqs] Processed 671030 reads in 152.890 CPU sec, 4.847 real sec
sorting block 121, #reads = 1069502 mem = 536882536 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279532, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 381, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (398.75, 174.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672232 sequences (100000125 bp) in 2.929 CPU sec, 3.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279463, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 378, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (396.78, 175.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673320 sequences (100000009 bp) in 2.188 CPU sec, 2.188 real sec...
[M::mem_process_seqs] Processed 671402 reads in 207.041 CPU sec, 6.603 real sec
[M::mem_process_seqs] Processed 671618 reads in 136.289 CPU sec, 4.305 real sec
sorting block 122, #reads = 1069180 mem = 536878997 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279582, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 371, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (388.99, 171.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (335, 4944, 6166)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17828)
[M::mem_pestat] mean and std.dev: (3926.90, 2405.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 23659)
[M::mem_pestat] skip orientation RR
[M::process] read 673140 sequences (100000070 bp) in 3.463 CPU sec, 3.746 real sec...
[M::process] read 673426 sequences (100000224 bp) in 2.231 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279450, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 364, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (373.19, 156.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672232 reads in 227.086 CPU sec, 7.219 real sec
[M::mem_process_seqs] Processed 673320 reads in 145.929 CPU sec, 4.628 real sec
sorting block 123, #reads = 1065580 mem = 536880708 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280215, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 366, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (375.06, 156.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672590 sequences (100000032 bp) in 2.817 CPU sec, 2.993 real sec...
[M::process] read 671640 sequences (100000102 bp) in 2.261 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279758, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (368.62, 155.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673140 reads in 219.519 CPU sec, 7.000 real sec
sorting block 124, #reads = 1062536 mem = 536890497 peak = 822071296
[M::mem_process_seqs] Processed 673426 reads in 141.552 CPU sec, 4.500 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279284, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 125, #reads = 1063950 mem = 536880175 peak = 822071296
[M::mem_pestat] (25, 50, 75) percentile: (255, 368, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (377.92, 157.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673064 sequences (100000249 bp) in 3.448 CPU sec, 3.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279501, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 384, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (390.27, 160.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673350 sequences (100000269 bp) in 2.134 CPU sec, 2.139 real sec...
[M::mem_process_seqs] Processed 672590 reads in 209.022 CPU sec, 6.643 real sec
[M::mem_process_seqs] Processed 671640 reads in 133.780 CPU sec, 4.230 real sec
sorting block 126, #reads = 1065354 mem = 536881037 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280517, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 364, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (376.62, 160.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673504 sequences (100000146 bp) in 3.266 CPU sec, 3.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279966, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 359, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (372.75, 159.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672916 sequences (100000284 bp) in 2.101 CPU sec, 2.099 real sec...
[M::mem_process_seqs] Processed 673064 reads in 194.968 CPU sec, 6.181 real sec
[M::mem_process_seqs] Processed 673350 reads in 123.665 CPU sec, 3.905 real sec
sorting block 127, #reads = 1066711 mem = 536878989 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279769, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (363.74, 153.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672638 sequences (100000136 bp) in 3.381 CPU sec, 3.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279802, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (368.92, 155.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671126 sequences (100000268 bp) in 2.028 CPU sec, 2.031 real sec...
[M::mem_process_seqs] Processed 673504 reads in 196.135 CPU sec, 6.222 real sec
sorting block 128, #reads = 1069307 mem = 536879558 peak = 822071296
[M::mem_process_seqs] Processed 672916 reads in 122.630 CPU sec, 3.868 real sec
sorting block 129, #reads = 1068507 mem = 536880561 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279713, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 361, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (373.63, 157.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671830 sequences (100000120 bp) in 2.862 CPU sec, 2.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279017, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 379, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (388.25, 158.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672034 sequences (100000240 bp) in 2.040 CPU sec, 2.038 real sec...
[M::mem_process_seqs] Processed 672638 reads in 182.250 CPU sec, 5.775 real sec
[M::mem_process_seqs] Processed 671126 reads in 114.842 CPU sec, 3.630 real sec
sorting block 130, #reads = 1068764 mem = 536880378 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279215, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 368, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (380.61, 157.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671732 sequences (100000048 bp) in 3.122 CPU sec, 3.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279079, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 367, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (380.25, 159.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671830 reads in 182.317 CPU sec, 5.792 real sec
[M::process] read 671678 sequences (100000249 bp) in 2.079 CPU sec, 2.090 real sec...
[M::mem_process_seqs] Processed 672034 reads in 114.931 CPU sec, 3.622 real sec
sorting block 131, #reads = 1069989 mem = 536886739 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278657, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 372, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (383.50, 159.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671650 sequences (100000144 bp) in 3.179 CPU sec, 3.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278684, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 372, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (384.27, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671418 sequences (100000204 bp) in 2.020 CPU sec, 2.027 real sec...
[M::mem_process_seqs] Processed 671732 reads in 181.309 CPU sec, 5.742 real sec
[M::mem_process_seqs] Processed 671678 reads in 113.745 CPU sec, 3.592 real sec
sorting block 132, #reads = 1069002 mem = 536881205 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278684, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 369, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (382.24, 160.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671434 sequences (100000217 bp) in 2.888 CPU sec, 3.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278359, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (391.74, 165.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671310 sequences (100000159 bp) in 2.020 CPU sec, 2.022 real sec...
[M::mem_process_seqs] Processed 671650 reads in 180.344 CPU sec, 5.722 real sec
sorting block 133, #reads = 1071034 mem = 536879177 peak = 822071296
[M::mem_process_seqs] Processed 671418 reads in 111.747 CPU sec, 3.529 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278413, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (386.65, 162.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 134, #reads = 1070973 mem = 536879748 peak = 822071296
[M::process] read 671188 sequences (100000183 bp) in 3.095 CPU sec, 3.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278061, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (392.30, 166.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671434 reads in 176.642 CPU sec, 5.592 real sec
[M::process] read 671276 sequences (100000229 bp) in 2.070 CPU sec, 2.068 real sec...
[M::mem_process_seqs] Processed 671310 reads in 111.120 CPU sec, 3.506 real sec
sorting block 135, #reads = 1071460 mem = 536880276 peak = 822071296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277691, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (392.46, 166.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671070 sequences (100000021 bp) in 3.107 CPU sec, 3.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278572, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (395.07, 168.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671236 sequences (100000107 bp) in 2.000 CPU sec, 1.998 real sec...
[M::mem_process_seqs] Processed 671188 reads in 179.042 CPU sec, 5.666 real sec
[M::mem_process_seqs] Processed 671276 reads in 111.818 CPU sec, 3.534 real sec
sorting block 136, #reads = 1070807 mem = 536884264 peak = 822071296
[M::process] read 191910 sequences (28555811 bp) in 1.449 CPU sec, 1.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278503, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (398.56, 170.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278871, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 380, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (397.49, 172.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 79475, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 372, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (389.61, 171.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671070 reads in 193.007 CPU sec, 6.232 real sec
[M::mem_process_seqs] Processed 671236 reads in 129.838 CPU sec, 4.192 real sec
[M::mem_process_seqs] Processed 191910 reads in 134.312 CPU sec, 4.347 real sec
sorting block 137, #reads = 1072010 mem = 536880664 peak = 822071296
sorting block 138, #reads = 1071561 mem = 536877598 peak = 822071296
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 148440098
overall: 69607718912 mem 24838.352 user 783.849 sys 852.660 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_Kh-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tLB:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 852.694 sec; CPU: 25622.205 sec
sorting block 139, #reads = 22046 mem = 11126550 peak = 822071296
uniq header count 1
merging 140 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 8358455200
output reads: 149162951
bam_mem_sort: 140 calls 118.020 user 1.794 sys 121.272 real
bam_write: 140 calls 77.592 user 7.461 sys 123.737 real
execute: 1 calls 42.544 user 2.367 sys 906.236 real
merge_files: 1 calls 42.523 user 2.346 sys 53.519 real
parse_chunk: 2225 calls 105.789 user 29.175 sys 151.200 real
read_chunk: 5992 calls 69.493 user 35.583 sys 766.560 real
sort_block: 1 calls 54.490 user 34.831 sys 743.598 real
write_chunk: 3765 calls 42.139 user 2.046 sys 52.582 real
overall: 2820628480 mem 2165.606 user 110.441 sys 906.255 real
ls: cannot access '*.cram': No such file or directory