.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L005;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b5/e4bb904f67dbe9f6951db49869051e/.command.log
Size: 129.6 KB
Task: DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L005;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b5/e4bb904f67dbe9f6951db49869051e/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/78284c27febfea84d0b5bd20dc25cc/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b5/e4bb904f67dbe9f6951db49869051e/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cf/78284c27febfea84d0b5bd20dc25cc/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1570.721 CPU sec, 55.259 real sec
[M::process] read 703478 sequences (100000237 bp) in 0.951 CPU sec, 0.952 real sec...
[M::process] read 701970 sequences (100000298 bp) in 1.150 CPU sec, 1.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292548, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (234.29, 109.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701732 sequences (100000064 bp) in 1.171 CPU sec, 1.179 real sec...
[M::process] read 700888 sequences (100000037 bp) in 1.159 CPU sec, 1.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292004, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (239.97, 113.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (310, 450, 751)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1633)
[M::mem_pestat] mean and std.dev: (477.36, 341.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2074)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 703478 reads in 129.918 CPU sec, 4.129 real sec
[M::mem_process_seqs] Processed 701970 reads in 78.773 CPU sec, 2.506 real sec
sorting block 0, #reads = 1095208 mem = 536877809 peak = 612462592
[M::process] read 700608 sequences (100000273 bp) in 1.530 CPU sec, 1.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292188, 6, 20)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.55, 115.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 429, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (297.56, 134.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RR
[M::process] read 700190 sequences (100000278 bp) in 1.190 CPU sec, 1.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291289, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (244.92, 117.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291749, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (246.66, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701732 reads in 181.394 CPU sec, 5.842 real sec
[M::mem_process_seqs] Processed 700888 reads in 135.248 CPU sec, 4.382 real sec
[M::mem_process_seqs] Processed 700608 reads in 146.518 CPU sec, 4.761 real sec
sorting block 1, #reads = 1095515 mem = 536878605 peak = 738291712
[M::process] read 699146 sequences (100000214 bp) in 1.749 CPU sec, 1.889 real sec...
sorting block 2, #reads = 1094934 mem = 536878130 peak = 838283264
[M::process] read 698978 sequences (100000119 bp) in 1.434 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291569, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.54, 120.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699146 reads in 70.649 CPU sec, 2.223 real sec
[M::process] read 698772 sequences (100000196 bp) in 1.496 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291637, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 1920, 2697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7731)
[M::mem_pestat] mean and std.dev: (1228.11, 1162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10248)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.83, 119.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291130, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (251.82, 121.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700190 reads in 200.245 CPU sec, 6.368 real sec
[M::mem_process_seqs] Processed 698978 reads in 94.095 CPU sec, 2.992 real sec
sorting block 3, #reads = 1094516 mem = 536880533 peak = 838283264
[M::process] read 698068 sequences (100000287 bp) in 1.509 CPU sec, 1.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290853, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.14, 121.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 346, 2660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7640)
[M::mem_pestat] mean and std.dev: (1186.50, 1204.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10130)
[M::mem_pestat] skip orientation RR
sorting block 4, #reads = 1095617 mem = 536878698 peak = 871182336
[M::process] read 697268 sequences (100000048 bp) in 1.934 CPU sec, 2.061 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290841, 12, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.80, 122.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (303, 388, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 558)
[M::mem_pestat] mean and std.dev: (370.64, 103.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 696240 sequences (100000195 bp) in 1.290 CPU sec, 1.288 real sec...
[M::mem_process_seqs] Processed 698772 reads in 132.585 CPU sec, 4.235 real sec
[M::mem_process_seqs] Processed 698068 reads in 83.114 CPU sec, 2.643 real sec
sorting block 5, #reads = 1093749 mem = 536879560 peak = 871182336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291043, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (254.64, 121.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696514 sequences (100000211 bp) in 2.087 CPU sec, 2.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290139, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 183, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (202.44, 64.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.29, 120.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 364, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (320.30, 70.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 696322 sequences (100000079 bp) in 1.313 CPU sec, 1.326 real sec...
[M::mem_process_seqs] Processed 697268 reads in 131.743 CPU sec, 4.202 real sec
[M::mem_process_seqs] Processed 696240 reads in 82.254 CPU sec, 2.614 real sec
sorting block 6, #reads = 1093116 mem = 536880611 peak = 871182336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290767, 9, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.21, 120.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 236, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (217.82, 68.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] skip orientation RR
[M::process] read 696140 sequences (100000105 bp) in 1.995 CPU sec, 2.111 real sec...
[M::process] read 695898 sequences (100000051 bp) in 1.340 CPU sec, 1.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289981, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.00, 119.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696514 reads in 132.965 CPU sec, 4.244 real sec
sorting block 7, #reads = 1092464 mem = 536879809 peak = 871182336
[M::mem_process_seqs] Processed 696322 reads in 83.623 CPU sec, 2.665 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290007, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (252.54, 117.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696524 sequences (100000020 bp) in 2.055 CPU sec, 2.160 real sec...
[M::process] read 699250 sequences (100000261 bp) in 1.400 CPU sec, 1.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290138, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.57, 120.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696140 reads in 135.682 CPU sec, 4.331 real sec
sorting block 8, #reads = 1091403 mem = 536880626 peak = 871182336
[M::mem_process_seqs] Processed 695898 reads in 85.234 CPU sec, 2.715 real sec
sorting block 9, #reads = 1090026 mem = 536883178 peak = 904212480
[M::process] read 702538 sequences (100000062 bp) in 2.106 CPU sec, 2.214 real sec...
[M::process] read 701454 sequences (100000043 bp) in 1.360 CPU sec, 1.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291591, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.30, 118.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699250 reads in 74.177 CPU sec, 2.343 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290158, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.43, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292263, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (238.11, 112.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696524 reads in 208.416 CPU sec, 6.629 real sec
[M::process] read 701014 sequences (100000097 bp) in 1.693 CPU sec, 1.701 real sec...
sorting block 10, #reads = 1090821 mem = 536877413 peak = 904212480
[M::mem_process_seqs] Processed 702538 reads in 96.055 CPU sec, 3.070 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291791, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.93, 115.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1091873 mem = 536881236 peak = 908779520
[M::process] read 700528 sequences (100000174 bp) in 2.378 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291485, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.08, 116.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701454 reads in 131.710 CPU sec, 4.201 real sec
[M::process] read 700344 sequences (100000229 bp) in 1.461 CPU sec, 1.470 real sec...
[M::mem_process_seqs] Processed 701014 reads in 82.828 CPU sec, 2.623 real sec
sorting block 12, #reads = 1096213 mem = 536878067 peak = 908779520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291618, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.63, 116.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 622, 696)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1826)
[M::mem_pestat] mean and std.dev: (444.50, 304.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2391)
[M::mem_pestat] skip orientation RR
[M::process] read 699878 sequences (100000051 bp) in 2.097 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291885, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.03, 118.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699484 sequences (100000003 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 700528 reads in 133.993 CPU sec, 4.280 real sec
[M::mem_process_seqs] Processed 700344 reads in 84.752 CPU sec, 2.693 real sec
sorting block 13, #reads = 1095012 mem = 536877709 peak = 908779520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291498, 0, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (250.27, 126.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.88, 119.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 170, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (186.60, 90.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698882 sequences (100000203 bp) in 2.241 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291376, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.78, 118.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698654 sequences (100000118 bp) in 1.420 CPU sec, 1.423 real sec...
[M::mem_process_seqs] Processed 699878 reads in 135.779 CPU sec, 4.330 real sec
sorting block 14, #reads = 1095805 mem = 536879031 peak = 908779520
[M::mem_process_seqs] Processed 699484 reads in 83.381 CPU sec, 2.653 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291257, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.40, 119.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1094953 mem = 536879138 peak = 908779520
[M::process] read 698498 sequences (100000112 bp) in 1.982 CPU sec, 2.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291506, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.39, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698882 reads in 127.779 CPU sec, 4.080 real sec
[M::process] read 697686 sequences (100000011 bp) in 1.541 CPU sec, 1.563 real sec...
[M::mem_process_seqs] Processed 698654 reads in 80.536 CPU sec, 2.541 real sec
sorting block 16, #reads = 1094456 mem = 536879974 peak = 908779520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291256, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.09, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696820 sequences (100000238 bp) in 2.229 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291102, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.53, 120.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698498 reads in 129.225 CPU sec, 4.112 real sec
[M::process] read 697162 sequences (100000144 bp) in 1.509 CPU sec, 1.512 real sec...
[M::mem_process_seqs] Processed 697686 reads in 82.887 CPU sec, 2.616 real sec
sorting block 17, #reads = 1094059 mem = 536878257 peak = 908779520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290268, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.25, 119.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697352 sequences (100000003 bp) in 2.004 CPU sec, 2.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290397, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.96, 119.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696798 sequences (100000164 bp) in 1.400 CPU sec, 1.408 real sec...
[M::mem_process_seqs] Processed 696820 reads in 130.618 CPU sec, 4.161 real sec
[M::mem_process_seqs] Processed 697162 reads in 82.505 CPU sec, 2.610 real sec
sorting block 18, #reads = 1093056 mem = 536878574 peak = 908779520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290590, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.77, 119.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696722 sequences (100000253 bp) in 2.333 CPU sec, 2.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290502, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.52, 118.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697352 reads in 131.112 CPU sec, 4.174 real sec
sorting block 19, #reads = 1091997 mem = 536879012 peak = 908779520
[M::process] read 696410 sequences (100000099 bp) in 1.542 CPU sec, 1.559 real sec...
[M::mem_process_seqs] Processed 696798 reads in 83.026 CPU sec, 2.621 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289983, 13, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.70, 120.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (62, 90, 121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239)
[M::mem_pestat] mean and std.dev: (79.73, 28.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 886, 915)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2327)
[M::mem_pestat] mean and std.dev: (711.83, 497.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3033)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 20, #reads = 1091900 mem = 536878036 peak = 927195136
[M::process] read 700670 sequences (100000253 bp) in 1.922 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290249, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.09, 120.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696722 reads in 132.116 CPU sec, 4.215 real sec
[M::process] read 702844 sequences (100000032 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_process_seqs] Processed 696410 reads in 84.156 CPU sec, 2.663 real sec
sorting block 21, #reads = 1091472 mem = 536880227 peak = 927195136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291719, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (243.03, 115.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700912 sequences (100000161 bp) in 2.308 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291985, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (238.37, 112.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700590 sequences (100000120 bp) in 1.420 CPU sec, 1.415 real sec...
[M::mem_process_seqs] Processed 700670 reads in 132.478 CPU sec, 4.208 real sec
[M::mem_process_seqs] Processed 702844 reads in 83.397 CPU sec, 2.642 real sec
sorting block 22, #reads = 1091298 mem = 536878596 peak = 927195136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291432, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (243.89, 116.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 283, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (132, 487)
[M::mem_pestat] mean and std.dev: (265.25, 69.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 558)
[M::mem_pestat] skip orientation RR
[M::process] read 700102 sequences (100000175 bp) in 1.944 CPU sec, 2.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292236, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.68, 117.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699936 sequences (100000206 bp) in 1.390 CPU sec, 1.395 real sec...
[M::mem_process_seqs] Processed 700912 reads in 129.551 CPU sec, 4.140 real sec
sorting block 23, #reads = 1094837 mem = 536879169 peak = 927195136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291424, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 244, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (251.14, 73.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.67, 119.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699202 sequences (100000248 bp) in 1.840 CPU sec, 1.846 real sec...
[M::mem_process_seqs] Processed 700102 reads in 71.972 CPU sec, 2.276 real sec
sorting block 24, #reads = 1094422 mem = 536889921 peak = 937463808
[M::mem_process_seqs] Processed 700590 reads in 183.120 CPU sec, 5.819 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291770, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.81, 120.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698482 sequences (100000006 bp) in 1.994 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291273, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (97, 233, 983)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2755)
[M::mem_pestat] mean and std.dev: (477.50, 430.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3641)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.37, 121.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699936 reads in 140.380 CPU sec, 4.464 real sec
[M::mem_process_seqs] Processed 699202 reads in 81.691 CPU sec, 2.583 real sec
sorting block 25, #reads = 1094330 mem = 536889999 peak = 937463808
[M::process] read 699340 sequences (100000199 bp) in 1.731 CPU sec, 1.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291092, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.62, 121.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698066 sequences (100000118 bp) in 2.137 CPU sec, 2.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290961, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.76, 119.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698482 reads in 129.099 CPU sec, 4.113 real sec
sorting block 26, #reads = 1094743 mem = 536883278 peak = 937463808
[M::mem_process_seqs] Processed 699340 reads in 81.739 CPU sec, 2.580 real sec
[M::process] read 697204 sequences (100000094 bp) in 1.566 CPU sec, 1.589 real sec...
sorting block 27, #reads = 1093886 mem = 536877930 peak = 1004204032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290898, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.63, 121.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696956 sequences (100000088 bp) in 2.017 CPU sec, 2.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290763, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.48, 121.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698066 reads in 130.534 CPU sec, 4.171 real sec
[M::process] read 696836 sequences (100000141 bp) in 1.485 CPU sec, 1.502 real sec...
[M::mem_process_seqs] Processed 697204 reads in 82.737 CPU sec, 2.620 real sec
sorting block 28, #reads = 1094194 mem = 536879246 peak = 1004204032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290385, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.37, 120.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697474 sequences (100000220 bp) in 2.083 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290482, 11, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.43, 119.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 82, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (187.00, 253.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 696956 reads in 130.669 CPU sec, 4.167 real sec
[M::process] read 697462 sequences (100000296 bp) in 1.492 CPU sec, 1.494 real sec...
[M::mem_process_seqs] Processed 696836 reads in 82.358 CPU sec, 2.602 real sec
sorting block 29, #reads = 1092824 mem = 536879709 peak = 1004204032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290768, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.94, 119.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696530 sequences (100000048 bp) in 1.955 CPU sec, 2.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290622, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.56, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696650 sequences (100000204 bp) in 1.420 CPU sec, 1.418 real sec...
[M::mem_process_seqs] Processed 697474 reads in 132.127 CPU sec, 4.209 real sec
sorting block 30, #reads = 1093399 mem = 536879793 peak = 1004204032
[M::mem_process_seqs] Processed 697462 reads in 82.813 CPU sec, 2.622 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290754, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 235, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (206.40, 69.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.35, 119.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 31, #reads = 1092057 mem = 536877529 peak = 1004204032
[M::process] read 697266 sequences (100000189 bp) in 2.330 CPU sec, 2.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290099, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.20, 120.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 1183, 1847)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5143)
[M::mem_pestat] mean and std.dev: (820.56, 716.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6791)
[M::mem_pestat] skip orientation RR
[M::process] read 704158 sequences (100000074 bp) in 1.429 CPU sec, 1.426 real sec...
[M::mem_process_seqs] Processed 696530 reads in 134.728 CPU sec, 4.284 real sec
[M::mem_process_seqs] Processed 696650 reads in 85.045 CPU sec, 2.693 real sec
sorting block 32, #reads = 1090776 mem = 536880098 peak = 1004204032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289999, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (253.65, 121.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701370 sequences (100000276 bp) in 1.960 CPU sec, 2.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292676, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (233.22, 109.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700376 sequences (100000226 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_process_seqs] Processed 697266 reads in 138.430 CPU sec, 4.415 real sec
[M::mem_process_seqs] Processed 704158 reads in 84.609 CPU sec, 2.682 real sec
sorting block 33, #reads = 1088789 mem = 536883405 peak = 1004204032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291901, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (241.97, 115.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700196 sequences (100000286 bp) in 2.105 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290930, 6, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.94, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 187, 1086)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2976)
[M::mem_pestat] mean and std.dev: (578.77, 755.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3921)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701370 reads in 129.551 CPU sec, 4.125 real sec
[M::process] read 699516 sequences (100000070 bp) in 1.429 CPU sec, 1.424 real sec...
[M::mem_process_seqs] Processed 700376 reads in 80.622 CPU sec, 2.547 real sec
sorting block 34, #reads = 1097141 mem = 536877596 peak = 1004204032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291524, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.28, 119.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699292 sequences (100000030 bp) in 1.989 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291262, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 332, 1320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3538)
[M::mem_pestat] mean and std.dev: (426.75, 367.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4647)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.57, 120.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 495, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657)
[M::mem_pestat] mean and std.dev: (448.00, 355.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2145)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700196 reads in 126.485 CPU sec, 4.035 real sec
sorting block 35, #reads = 1094223 mem = 536886999 peak = 1004204032
[M::mem_process_seqs] Processed 699516 reads in 80.567 CPU sec, 2.539 real sec
[M::process] read 698504 sequences (100000067 bp) in 1.531 CPU sec, 1.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291520, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.30, 121.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1094683 mem = 536879885 peak = 1056669696
[M::process] read 698714 sequences (100000083 bp) in 2.276 CPU sec, 2.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291402, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.58, 122.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699292 reads in 127.552 CPU sec, 4.059 real sec
[M::mem_process_seqs] Processed 698504 reads in 80.918 CPU sec, 2.548 real sec
[M::process] read 698320 sequences (100000145 bp) in 1.561 CPU sec, 1.589 real sec...
sorting block 37, #reads = 1095506 mem = 536883497 peak = 1056669696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291333, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.67, 121.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696848 sequences (100000072 bp) in 1.939 CPU sec, 2.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290867, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.82, 121.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698714 reads in 128.777 CPU sec, 4.103 real sec
[M::process] read 696502 sequences (100000051 bp) in 1.508 CPU sec, 1.516 real sec...
[M::mem_process_seqs] Processed 698320 reads in 82.196 CPU sec, 2.589 real sec
sorting block 38, #reads = 1093976 mem = 536878510 peak = 1056669696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290233, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.41, 121.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696476 sequences (100000003 bp) in 2.264 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290694, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.46, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696848 reads in 129.811 CPU sec, 4.126 real sec
[M::process] read 696864 sequences (100000198 bp) in 1.462 CPU sec, 1.475 real sec...
sorting block 39, #reads = 1093401 mem = 536877427 peak = 1056669696
[M::mem_process_seqs] Processed 696502 reads in 82.655 CPU sec, 2.606 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290569, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (252.01, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1092375 mem = 536877974 peak = 1056669696
[M::process] read 696568 sequences (100000273 bp) in 1.966 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290010, 10, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (324, 498, 1600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4152)
[M::mem_pestat] mean and std.dev: (660.27, 611.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5428)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.10, 118.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 527, 782)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1892)
[M::mem_pestat] mean and std.dev: (549.89, 459.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2447)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (286, 638, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738)
[M::mem_pestat] mean and std.dev: (447.60, 240.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2222)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696476 reads in 130.532 CPU sec, 4.159 real sec
[M::process] read 696856 sequences (100000238 bp) in 1.462 CPU sec, 1.472 real sec...
[M::mem_process_seqs] Processed 696864 reads in 82.217 CPU sec, 2.596 real sec
sorting block 41, #reads = 1091157 mem = 536878036 peak = 1056669696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290331, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.03, 118.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696518 sequences (100000298 bp) in 2.232 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290645, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (287, 395, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 611)
[M::mem_pestat] mean and std.dev: (320.64, 80.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 719)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.09, 119.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696568 reads in 130.408 CPU sec, 4.141 real sec
[M::process] read 696144 sequences (100000281 bp) in 1.420 CPU sec, 1.423 real sec...
[M::mem_process_seqs] Processed 696856 reads in 82.539 CPU sec, 2.604 real sec
sorting block 42, #reads = 1092233 mem = 536876904 peak = 1056669696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290480, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 494, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138)
[M::mem_pestat] mean and std.dev: (332.88, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.20, 121.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 184, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (258.13, 143.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 700762 sequences (100000091 bp) in 1.968 CPU sec, 2.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290366, 11, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (255.53, 121.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 220, 750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2046)
[M::mem_pestat] mean and std.dev: (459.91, 459.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2694)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 696518 reads in 129.661 CPU sec, 4.124 real sec
[M::process] read 705568 sequences (100000224 bp) in 1.489 CPU sec, 1.495 real sec...
[M::mem_process_seqs] Processed 696144 reads in 82.245 CPU sec, 2.595 real sec
sorting block 43, #reads = 1090801 mem = 536880721 peak = 1056669696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291804, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (243.09, 116.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705384 sequences (100000024 bp) in 2.345 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293343, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.94, 108.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704330 sequences (100000022 bp) in 1.420 CPU sec, 1.418 real sec...
[M::mem_process_seqs] Processed 700762 reads in 138.988 CPU sec, 4.410 real sec
sorting block 44, #reads = 1091281 mem = 536881048 peak = 1056669696
[M::mem_process_seqs] Processed 705568 reads in 87.475 CPU sec, 2.765 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293236, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 45, #reads = 1091994 mem = 536879791 peak = 1065603072
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (233.03, 111.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702838 sequences (100000245 bp) in 2.047 CPU sec, 2.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293179, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 191, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1514)
[M::mem_pestat] mean and std.dev: (347.00, 345.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1967)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.30, 113.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702584 sequences (100000250 bp) in 1.430 CPU sec, 1.429 real sec...
[M::mem_process_seqs] Processed 705384 reads in 135.871 CPU sec, 4.327 real sec
[M::mem_process_seqs] Processed 704330 reads in 85.299 CPU sec, 2.694 real sec
sorting block 46, #reads = 1094574 mem = 536877271 peak = 1065603072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292272, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.74, 116.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (103, 221, 2056)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5962)
[M::mem_pestat] mean and std.dev: (794.10, 921.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7915)
[M::mem_pestat] skip orientation RR
[M::process] read 701844 sequences (100000219 bp) in 2.379 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292080, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (241.87, 117.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701510 sequences (100000112 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_process_seqs] Processed 702838 reads in 133.472 CPU sec, 4.234 real sec
[M::mem_process_seqs] Processed 702584 reads in 84.044 CPU sec, 2.656 real sec
sorting block 47, #reads = 1095453 mem = 536879199 peak = 1065603072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292645, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 157, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361)
[M::mem_pestat] mean and std.dev: (147.44, 37.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.74, 118.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701594 sequences (100000225 bp) in 2.012 CPU sec, 2.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291977, 10, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.44, 118.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 364, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240)
[M::mem_pestat] mean and std.dev: (364.67, 317.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1634)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 297, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (72, 552)
[M::mem_pestat] mean and std.dev: (266.60, 78.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701844 reads in 132.224 CPU sec, 4.208 real sec
[M::process] read 701166 sequences (100000046 bp) in 1.440 CPU sec, 1.436 real sec...
sorting block 48, #reads = 1094941 mem = 536884592 peak = 1065603072
[M::mem_process_seqs] Processed 701510 reads in 83.887 CPU sec, 2.648 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 292412, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (245.13, 119.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1094271 mem = 536883984 peak = 1065603072
[M::process] read 701038 sequences (100000275 bp) in 2.314 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292157, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.73, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 220, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (252.90, 99.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RR
[M::process] read 700630 sequences (100000067 bp) in 1.430 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 701594 reads in 134.596 CPU sec, 4.271 real sec
[M::mem_process_seqs] Processed 701166 reads in 85.194 CPU sec, 2.695 real sec
sorting block 50, #reads = 1094036 mem = 536882096 peak = 1065603072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291641, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.60, 119.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699332 sequences (100000042 bp) in 2.020 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291516, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.22, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698288 sequences (100000212 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_process_seqs] Processed 701038 reads in 135.726 CPU sec, 4.319 real sec
[M::mem_process_seqs] Processed 700630 reads in 86.269 CPU sec, 2.724 real sec
sorting block 51, #reads = 1092659 mem = 536877798 peak = 1065603072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291363, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 340, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (290.22, 154.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.04, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 1231, 1861)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5315)
[M::mem_pestat] mean and std.dev: (915.56, 758.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7042)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698112 sequences (100000185 bp) in 2.384 CPU sec, 2.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 291056, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.51, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697506 sequences (100000141 bp) in 1.430 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 699332 reads in 137.337 CPU sec, 4.358 real sec
[M::mem_process_seqs] Processed 698288 reads in 86.483 CPU sec, 2.739 real sec
sorting block 52, #reads = 1093062 mem = 536878492 peak = 1065603072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290764, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 203, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (179.60, 67.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.85, 119.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697846 sequences (100000003 bp) in 2.170 CPU sec, 2.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291082, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (253.00, 121.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698112 reads in 138.221 CPU sec, 4.398 real sec
sorting block 53, #reads = 1091234 mem = 536882626 peak = 1065603072
[M::process] read 706504 sequences (100000207 bp) in 1.557 CPU sec, 1.563 real sec...
[M::mem_process_seqs] Processed 697506 reads in 87.935 CPU sec, 2.774 real sec
sorting block 54, #reads = 1090680 mem = 536877601 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290923, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.46, 121.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 444, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (329.38, 131.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RR
[M::process] read 709960 sequences (100000214 bp) in 2.505 CPU sec, 2.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293446, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (228.89, 107.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 736)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708564 sequences (100000161 bp) in 1.500 CPU sec, 1.501 real sec...
[M::mem_process_seqs] Processed 697846 reads in 147.417 CPU sec, 4.678 real sec
[M::mem_process_seqs] Processed 706504 reads in 98.324 CPU sec, 3.114 real sec
sorting block 55, #reads = 1090704 mem = 536878623 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 294641, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 199, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (221.01, 102.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 700)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706774 sequences (100000084 bp) in 2.164 CPU sec, 2.246 real sec...
[M::process] read 705110 sequences (100000240 bp) in 1.500 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293866, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.46, 105.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 317, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 585)
[M::mem_pestat] mean and std.dev: (301.70, 98.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 709960 reads in 156.826 CPU sec, 4.990 real sec
[M::mem_process_seqs] Processed 708564 reads in 98.114 CPU sec, 3.108 real sec
sorting block 56, #reads = 1087996 mem = 536882270 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 294069, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 299, 1567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4353)
[M::mem_pestat] mean and std.dev: (769.10, 1128.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5746)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.50, 108.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703780 sequences (100000147 bp) in 2.463 CPU sec, 2.565 real sec...
[M::process] read 703350 sequences (100000068 bp) in 1.500 CPU sec, 1.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293228, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 324, 883)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2231)
[M::mem_pestat] mean and std.dev: (513.00, 344.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2905)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.75, 111.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 706774 reads in 151.003 CPU sec, 4.794 real sec
sorting block 57, #reads = 1090550 mem = 536881428 peak = 1137012736
[M::mem_process_seqs] Processed 705110 reads in 94.769 CPU sec, 3.004 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293034, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (236.39, 112.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1090755 mem = 536877963 peak = 1137012736
[M::process] read 702658 sequences (100000231 bp) in 2.177 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292834, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 247, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105)
[M::mem_pestat] mean and std.dev: (295.92, 148.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1416)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.64, 114.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701964 sequences (100000186 bp) in 1.500 CPU sec, 1.497 real sec...
[M::mem_process_seqs] Processed 703780 reads in 147.899 CPU sec, 4.710 real sec
[M::mem_process_seqs] Processed 703350 reads in 92.478 CPU sec, 2.927 real sec
sorting block 59, #reads = 1090745 mem = 536877063 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293003, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.39, 115.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701578 sequences (100000124 bp) in 2.631 CPU sec, 2.695 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292295, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (240.98, 115.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700862 sequences (100000283 bp) in 1.490 CPU sec, 1.495 real sec...
[M::mem_process_seqs] Processed 702658 reads in 144.917 CPU sec, 4.594 real sec
[M::mem_process_seqs] Processed 701964 reads in 90.504 CPU sec, 2.859 real sec
sorting block 60, #reads = 1092809 mem = 536877268 peak = 1137012736
[M::process] read 699642 sequences (100000124 bp) in 2.391 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292062, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.77, 119.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700862 reads in 77.262 CPU sec, 2.428 real sec
[M::process] read 698600 sequences (100000025 bp) in 1.660 CPU sec, 1.688 real sec...
sorting block 61, #reads = 1091898 mem = 536879284 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291710, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (242.27, 116.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291418, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 203, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (191.78, 84.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.94, 120.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701578 reads in 219.276 CPU sec, 6.969 real sec
[M::mem_process_seqs] Processed 699642 reads in 103.757 CPU sec, 3.282 real sec
[M::process] read 698656 sequences (100000064 bp) in 2.006 CPU sec, 2.047 real sec...
sorting block 62, #reads = 1092167 mem = 536878808 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291132, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.45, 120.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698146 sequences (100000003 bp) in 2.418 CPU sec, 2.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290759, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.97, 120.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698600 reads in 140.492 CPU sec, 4.467 real sec
[M::process] read 699616 sequences (100000024 bp) in 1.640 CPU sec, 1.658 real sec...
[M::mem_process_seqs] Processed 698656 reads in 90.037 CPU sec, 2.840 real sec
sorting block 63, #reads = 1090987 mem = 536880688 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290772, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.33, 121.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705974 sequences (100000280 bp) in 2.325 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290745, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.54, 119.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 280, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198)
[M::mem_pestat] mean and std.dev: (316.92, 151.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1529)
[M::mem_pestat] skip orientation RR
[M::process] read 703610 sequences (100000068 bp) in 1.650 CPU sec, 1.653 real sec...
[M::mem_process_seqs] Processed 698146 reads in 150.798 CPU sec, 4.794 real sec
sorting block 64, #reads = 1089433 mem = 536879030 peak = 1137012736
[M::mem_process_seqs] Processed 699616 reads in 102.148 CPU sec, 3.231 real sec
sorting block 65, #reads = 1089262 mem = 536876180 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293141, 10, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.57, 108.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 343, 1071)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2939)
[M::mem_pestat] mean and std.dev: (583.50, 453.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3873)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 702952 sequences (100000249 bp) in 2.665 CPU sec, 2.783 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292737, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (236.40, 112.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705974 reads in 141.909 CPU sec, 4.505 real sec
[M::process] read 702428 sequences (100000256 bp) in 1.523 CPU sec, 1.539 real sec...
[M::mem_process_seqs] Processed 703610 reads in 88.837 CPU sec, 2.799 real sec
sorting block 66, #reads = 1087866 mem = 536878638 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292648, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.33, 114.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701658 sequences (100000085 bp) in 2.574 CPU sec, 2.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292694, 10, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.21, 114.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (55, 170, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1714)
[M::mem_pestat] mean and std.dev: (316.80, 288.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2267)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 188, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374)
[M::mem_pestat] mean and std.dev: (177.67, 50.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 452)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702952 reads in 137.545 CPU sec, 4.367 real sec
[M::mem_process_seqs] Processed 702428 reads in 86.000 CPU sec, 2.708 real sec
[M::process] read 700654 sequences (100000189 bp) in 1.747 CPU sec, 1.792 real sec...
sorting block 67, #reads = 1094566 mem = 536880406 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292129, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 185, 1062)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2886)
[M::mem_pestat] mean and std.dev: (549.50, 615.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3798)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.56, 116.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700418 sequences (100000163 bp) in 2.094 CPU sec, 2.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292015, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.72, 117.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701658 reads in 135.413 CPU sec, 4.321 real sec
sorting block 68, #reads = 1095399 mem = 536886020 peak = 1137012736
[M::process] read 701254 sequences (100000025 bp) in 1.599 CPU sec, 1.626 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291417, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.39, 118.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701190 sequences (100000139 bp) in 1.751 CPU sec, 1.754 real sec...
[M::mem_process_seqs] Processed 700418 reads in 78.787 CPU sec, 2.521 real sec
sorting block 69, #reads = 1094625 mem = 536878660 peak = 1137012736
[M::mem_process_seqs] Processed 700654 reads in 183.794 CPU sec, 5.849 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292428, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.05, 115.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699738 sequences (100000004 bp) in 2.648 CPU sec, 2.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291807, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.14, 117.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701254 reads in 149.088 CPU sec, 4.740 real sec
[M::mem_process_seqs] Processed 701190 reads in 88.801 CPU sec, 2.811 real sec
sorting block 70, #reads = 1095203 mem = 536881465 peak = 1137012736
[M::process] read 699320 sequences (100000187 bp) in 1.913 CPU sec, 2.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291298, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.25, 119.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 71, #reads = 1092815 mem = 536880305 peak = 1137012736
[M::process] read 699084 sequences (100000034 bp) in 2.113 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291261, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (314, 905, 967)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273)
[M::mem_pestat] mean and std.dev: (623.50, 344.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2926)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.06, 120.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699738 reads in 138.765 CPU sec, 4.421 real sec
[M::mem_process_seqs] Processed 699320 reads in 87.786 CPU sec, 2.777 real sec
[M::process] read 699094 sequences (100000049 bp) in 1.735 CPU sec, 1.775 real sec...
sorting block 72, #reads = 1093027 mem = 536878367 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291086, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.96, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698244 sequences (100000184 bp) in 2.420 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291309, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 337, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1530)
[M::mem_pestat] mean and std.dev: (368.92, 216.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1960)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.83, 119.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699084 reads in 139.042 CPU sec, 4.418 real sec
[M::process] read 698066 sequences (100000017 bp) in 1.491 CPU sec, 1.504 real sec...
[M::mem_process_seqs] Processed 699094 reads in 89.076 CPU sec, 2.812 real sec
sorting block 73, #reads = 1092130 mem = 536878800 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291083, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.50, 119.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 181, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289)
[M::mem_pestat] mean and std.dev: (266.92, 182.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1673)
[M::mem_pestat] skip orientation RR
[M::process] read 698188 sequences (100000045 bp) in 2.092 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290517, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 267, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (365.58, 252.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1374)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.82, 120.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700866 sequences (100000142 bp) in 1.530 CPU sec, 1.521 real sec...
[M::mem_process_seqs] Processed 698244 reads in 138.905 CPU sec, 4.414 real sec
[M::mem_process_seqs] Processed 698066 reads in 88.544 CPU sec, 2.800 real sec
sorting block 74, #reads = 1090121 mem = 536879729 peak = 1137012736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290157, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.52, 119.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 297, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (240.70, 80.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 700)
[M::mem_pestat] skip orientation RR
[M::process] read 711648 sequences (100000192 bp) in 2.391 CPU sec, 2.504 real sec...
[M::process] read 706940 sequences (100000078 bp) in 1.550 CPU sec, 1.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291327, 14, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (265, 283, 1653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4429)
[M::mem_pestat] mean and std.dev: (732.31, 960.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5817)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.93, 116.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (363, 1101, 1501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3777)
[M::mem_pestat] mean and std.dev: (820.00, 569.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4915)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 257, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144)
[M::mem_pestat] mean and std.dev: (275.70, 138.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1455)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698188 reads in 151.520 CPU sec, 4.811 real sec
sorting block 75, #reads = 1089086 mem = 536887855 peak = 1137012736
[M::process] read 705558 sequences (100000236 bp) in 1.972 CPU sec, 1.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 294842, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 196, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (217.50, 100.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 711648 reads in 89.246 CPU sec, 2.824 real sec
sorting block 76, #reads = 1087511 mem = 536878191 peak = 1155338240
[M::mem_process_seqs] Processed 700866 reads in 212.663 CPU sec, 6.749 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293955, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.47, 109.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703680 sequences (100000082 bp) in 2.431 CPU sec, 2.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293129, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (233.11, 111.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702516 sequences (100000031 bp) in 1.490 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 706940 reads in 169.363 CPU sec, 5.384 real sec
sorting block 77, #reads = 1082900 mem = 536884500 peak = 1155338240
[M::mem_process_seqs] Processed 705558 reads in 97.744 CPU sec, 3.089 real sec
sorting block 78, #reads = 1088974 mem = 536882711 peak = 1155338240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 293271, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.96, 115.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700916 sequences (100000035 bp) in 2.158 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292473, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (242.14, 117.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700982 sequences (100000030 bp) in 1.500 CPU sec, 1.498 real sec...
[M::mem_process_seqs] Processed 703680 reads in 148.588 CPU sec, 4.724 real sec
[M::mem_process_seqs] Processed 702516 reads in 93.183 CPU sec, 2.953 real sec
sorting block 79, #reads = 1088811 mem = 536880832 peak = 1155338240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291528, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (246.02, 119.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699306 sequences (100000050 bp) in 2.337 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291747, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (245.06, 118.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699318 sequences (100000046 bp) in 1.470 CPU sec, 1.478 real sec...
[M::mem_process_seqs] Processed 700916 reads in 142.732 CPU sec, 4.529 real sec
sorting block 80, #reads = 1089538 mem = 536879142 peak = 1155338240
[M::process] read 698326 sequences (100000053 bp) in 1.595 CPU sec, 1.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291612, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 193, 988)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2704)
[M::mem_pestat] mean and std.dev: (315.77, 338.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.34, 121.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699306 reads in 80.731 CPU sec, 2.774 real sec
[M::mem_process_seqs] Processed 700982 reads in 196.165 CPU sec, 6.440 real sec
sorting block 81, #reads = 1089770 mem = 536878090 peak = 1155338240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291597, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.83, 120.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698456 sequences (100000283 bp) in 2.274 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290648, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (249.36, 119.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699318 reads in 152.199 CPU sec, 4.834 real sec
[M::process] read 697780 sequences (100000087 bp) in 1.696 CPU sec, 1.772 real sec...
sorting block 82, #reads = 1090185 mem = 536876235 peak = 1155338240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290874, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.52, 120.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 238, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (234.89, 65.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RR
[M::process] read 697334 sequences (100000144 bp) in 1.458 CPU sec, 1.460 real sec...
[M::mem_process_seqs] Processed 698456 reads in 79.492 CPU sec, 2.523 real sec
[M::mem_process_seqs] Processed 698326 reads in 188.511 CPU sec, 5.975 real sec
sorting block 83, #reads = 1090101 mem = 536881801 peak = 1155338240
[M::process] read 697274 sequences (100000013 bp) in 2.276 CPU sec, 2.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290440, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 278, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (265.33, 165.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.31, 120.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697334 reads in 74.306 CPU sec, 2.342 real sec
[M::process] read 697050 sequences (100000010 bp) in 1.700 CPU sec, 1.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290907, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 84, #reads = 1089669 mem = 536880233 peak = 1155338240
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.26, 119.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290415, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (252.00, 120.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 483376 sequences (69290433 bp) in 1.241 CPU sec, 1.246 real sec...
[M::mem_process_seqs] Processed 697780 reads in 220.348 CPU sec, 6.975 real sec
[M::mem_process_seqs] Processed 697274 reads in 97.854 CPU sec, 3.107 real sec
sorting block 85, #reads = 1090774 mem = 536881376 peak = 1155338240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290329, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.58, 121.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 201034, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.75, 121.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697050 reads in 111.321 CPU sec, 3.610 real sec
[M::mem_process_seqs] Processed 483376 reads in 61.813 CPU sec, 1.998 real sec
sorting block 86, #reads = 1090136 mem = 536878215 peak = 1155338240
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 94987248
overall: 70072209408 mem 11323.660 user 317.484 sys 385.498 real
sorting block 87, #reads = 436338 mem = 215079461 peak = 1155338240
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 385.552 sec; CPU: 11641.166 sec
uniq header count 1
merging 88 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5272341581
output reads: 95470252
bam_mem_sort: 88 calls 35.989 user 0.598 sys 36.741 real
bam_write: 88 calls 19.710 user 0.933 sys 20.666 real
execute: 1 calls 18.050 user 1.016 sys 413.404 real
merge_files: 1 calls 18.043 user 0.982 sys 27.350 real
parse_chunk: 1399 calls 51.592 user 13.033 sys 65.534 real
read_chunk: 3779 calls 34.206 user 29.493 sys 338.455 real
sort_block: 1 calls 25.391 user 28.818 sys 325.682 real
write_chunk: 2378 calls 17.718 user 0.760 sys 26.543 real
overall: 3003662336 mem 1083.279 user 52.397 sys 413.429 real
ls: cannot access '*.cram': No such file or directory