.command.log - Workdir - DAQ:SENTIEON_BWAMEM (FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1;L002;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/69/e2645ce27331044b2dec43cbba8db8/.command.log
Size: 202.3 KB
Task: DAQ:SENTIEON_BWAMEM (FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1;L002;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/69/e2645ce27331044b2dec43cbba8db8/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5d/adf92f686291b4ec48f3565d0f5723/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/69/e2645ce27331044b2dec43cbba8db8/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5d/adf92f686291b4ec48f3565d0f5723/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1984.837 CPU sec, 74.767 real sec
[M::process] read 678112 sequences (100000169 bp) in 1.054 CPU sec, 1.061 real sec...
[M::process] read 676932 sequences (100000145 bp) in 1.511 CPU sec, 1.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281877, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 352, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (364.11, 164.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676398 sequences (100000185 bp) in 1.515 CPU sec, 1.581 real sec...
[M::process] read 675980 sequences (100000245 bp) in 1.540 CPU sec, 1.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281613, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 360, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (370.72, 166.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678112 reads in 169.912 CPU sec, 5.530 real sec
[M::mem_process_seqs] Processed 676932 reads in 105.770 CPU sec, 3.467 real sec
sorting block 0, #reads = 1066773 mem = 536880125 peak = 596901888
[M::process] read 675926 sequences (100000237 bp) in 1.907 CPU sec, 2.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281021, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 365, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (376.36, 168.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675846 sequences (100000006 bp) in 1.667 CPU sec, 1.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280371, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 372, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (381.47, 169.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280843, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 368, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (378.85, 168.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676398 reads in 262.982 CPU sec, 9.056 real sec
[M::mem_process_seqs] Processed 675926 reads in 198.970 CPU sec, 6.965 real sec
[M::mem_process_seqs] Processed 675980 reads in 213.600 CPU sec, 7.505 real sec
sorting block 1, #reads = 1066072 mem = 536879159 peak = 721158144
[M::process] read 675490 sequences (100000107 bp) in 1.811 CPU sec, 2.161 real sec...
sorting block 2, #reads = 1065445 mem = 536877467 peak = 819830784
[M::process] read 675362 sequences (100000123 bp) in 2.081 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281324, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (386.07, 171.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675490 reads in 105.904 CPU sec, 3.671 real sec
[M::process] read 675102 sequences (100000045 bp) in 1.674 CPU sec, 1.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281073, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (383.12, 170.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280396, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (387.03, 171.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675846 reads in 303.760 CPU sec, 10.612 real sec
[M::process] read 674820 sequences (100000005 bp) in 1.843 CPU sec, 1.908 real sec...
[M::mem_process_seqs] Processed 675362 reads in 141.091 CPU sec, 4.955 real sec
sorting block 3, #reads = 1065836 mem = 536880399 peak = 819830784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281102, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (388.24, 171.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674720 sequences (100000122 bp) in 2.078 CPU sec, 2.413 real sec...
sorting block 4, #reads = 1065095 mem = 536878438 peak = 826138624
[M::process] read 674874 sequences (100000270 bp) in 1.611 CPU sec, 1.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280251, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (389.66, 171.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675102 reads in 196.915 CPU sec, 6.927 real sec
[M::mem_process_seqs] Processed 674820 reads in 123.694 CPU sec, 4.292 real sec
sorting block 5, #reads = 1064819 mem = 536880671 peak = 826138624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280081, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (391.17, 172.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674906 sequences (100000120 bp) in 2.084 CPU sec, 2.568 real sec...
[M::process] read 674884 sequences (100000171 bp) in 1.705 CPU sec, 1.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280245, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 190, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (176.75, 67.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (391.70, 172.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674720 reads in 201.761 CPU sec, 7.220 real sec
[M::mem_process_seqs] Processed 674874 reads in 125.511 CPU sec, 4.475 real sec
sorting block 6, #reads = 1064552 mem = 536878087 peak = 826138624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280831, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (390.52, 172.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 330, 3105)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8853)
[M::mem_pestat] mean and std.dev: (1430.25, 1416.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11727)
[M::mem_pestat] skip orientation RR
[M::process] read 675038 sequences (100000115 bp) in 2.447 CPU sec, 2.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280511, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (389.37, 172.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675108 sequences (100000038 bp) in 1.701 CPU sec, 1.758 real sec...
[M::mem_process_seqs] Processed 674906 reads in 191.942 CPU sec, 6.816 real sec
[M::mem_process_seqs] Processed 674884 reads in 117.576 CPU sec, 4.111 real sec
sorting block 7, #reads = 1065030 mem = 536878524 peak = 826138624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279982, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (387.01, 171.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 192, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (117, 292)
[M::mem_pestat] mean and std.dev: (185.11, 36.20)
[M::mem_pestat] low and high boundaries for proper pairs: (40, 330)
[M::mem_pestat] skip orientation RR
[M::process] read 675180 sequences (100000172 bp) in 2.380 CPU sec, 3.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280406, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 374, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (384.90, 170.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675136 sequences (100000038 bp) in 1.643 CPU sec, 1.665 real sec...
[M::mem_process_seqs] Processed 675038 reads in 190.542 CPU sec, 6.700 real sec
sorting block 8, #reads = 1064435 mem = 536879872 peak = 826138624
[M::mem_process_seqs] Processed 675108 reads in 116.616 CPU sec, 4.114 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280682, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (385.16, 169.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1064999 mem = 536878549 peak = 854745088
[M::process] read 674860 sequences (100000032 bp) in 2.646 CPU sec, 2.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280115, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (385.48, 170.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674928 sequences (100000291 bp) in 1.753 CPU sec, 1.802 real sec...
[M::mem_process_seqs] Processed 675180 reads in 189.973 CPU sec, 6.491 real sec
[M::mem_process_seqs] Processed 675136 reads in 116.279 CPU sec, 3.930 real sec
sorting block 10, #reads = 1064510 mem = 536881614 peak = 854745088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280328, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (387.97, 170.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674822 sequences (100000138 bp) in 2.593 CPU sec, 2.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279930, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (387.77, 171.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674860 reads in 193.514 CPU sec, 6.534 real sec
[M::mem_process_seqs] Processed 674928 reads in 118.101 CPU sec, 4.005 real sec
[M::process] read 674588 sequences (100000035 bp) in 2.090 CPU sec, 2.327 real sec...
sorting block 11, #reads = 1065481 mem = 536878073 peak = 854745088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279725, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (386.55, 170.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673848 sequences (100000187 bp) in 2.388 CPU sec, 2.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279950, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (388.35, 170.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674822 reads in 187.782 CPU sec, 6.484 real sec
[M::process] read 674502 sequences (100000080 bp) in 1.777 CPU sec, 1.826 real sec...
[M::mem_process_seqs] Processed 674588 reads in 118.373 CPU sec, 4.089 real sec
sorting block 12, #reads = 1065952 mem = 536877905 peak = 854745088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279507, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 386, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (394.60, 170.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674312 sequences (100000053 bp) in 2.441 CPU sec, 2.891 real sec...
sorting block 13, #reads = 1065052 mem = 536880475 peak = 854745088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279353, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (387.10, 168.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673848 reads in 182.398 CPU sec, 6.226 real sec
[M::mem_process_seqs] Processed 674502 reads in 116.105 CPU sec, 3.908 real sec
[M::process] read 674308 sequences (100000070 bp) in 1.901 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280031, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 378, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (387.78, 168.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1064304 mem = 536880554 peak = 854745088
[M::process] read 674228 sequences (100000026 bp) in 2.457 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280126, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (303, 303, 812)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1830)
[M::mem_pestat] mean and std.dev: (525.27, 375.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2339)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 377, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (386.98, 168.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 307, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (250.08, 86.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674312 reads in 178.173 CPU sec, 6.106 real sec
[M::process] read 673916 sequences (100000072 bp) in 1.789 CPU sec, 1.836 real sec...
[M::mem_process_seqs] Processed 674308 reads in 112.174 CPU sec, 3.946 real sec
sorting block 15, #reads = 1065292 mem = 536879690 peak = 854745088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279784, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 379, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (388.86, 169.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 293, 809)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1943)
[M::mem_pestat] mean and std.dev: (420.90, 256.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2510)
[M::mem_pestat] skip orientation RR
[M::process] read 673810 sequences (100000114 bp) in 2.313 CPU sec, 2.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279840, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 381, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (390.95, 170.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674228 reads in 177.572 CPU sec, 5.939 real sec
[M::process] read 673572 sequences (100000089 bp) in 1.897 CPU sec, 2.016 real sec...
[M::mem_process_seqs] Processed 673916 reads in 112.547 CPU sec, 3.761 real sec
sorting block 16, #reads = 1065145 mem = 536880650 peak = 854986752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280103, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 385, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (395.00, 172.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673658 sequences (100000168 bp) in 2.679 CPU sec, 3.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280159, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 390, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (399.43, 174.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673810 reads in 171.653 CPU sec, 5.956 real sec
[M::mem_process_seqs] Processed 673572 reads in 110.237 CPU sec, 3.945 real sec
sorting block 17, #reads = 1065113 mem = 536881167 peak = 854986752
[M::process] read 676090 sequences (100000189 bp) in 2.123 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280488, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 389, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (398.34, 173.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676678 sequences (100000030 bp) in 2.375 CPU sec, 2.921 real sec...
sorting block 18, #reads = 1064420 mem = 536878308 peak = 865959936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281402, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 368, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (379.23, 170.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673658 reads in 172.894 CPU sec, 5.886 real sec
[M::mem_process_seqs] Processed 676090 reads in 114.149 CPU sec, 3.934 real sec
[M::process] read 676300 sequences (100000298 bp) in 1.884 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281135, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 364, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (376.23, 170.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1064122 mem = 536879569 peak = 865959936
[M::process] read 675840 sequences (100000238 bp) in 2.415 CPU sec, 2.962 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280766, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 372, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (382.19, 171.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676678 reads in 175.246 CPU sec, 5.909 real sec
[M::mem_process_seqs] Processed 676300 reads in 112.155 CPU sec, 3.730 real sec
[M::process] read 675854 sequences (100000240 bp) in 1.960 CPU sec, 2.129 real sec...
sorting block 20, #reads = 1067316 mem = 536877113 peak = 865959936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 279876, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (332, 408, 1503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3845)
[M::mem_pestat] mean and std.dev: (711.90, 523.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5016)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 374, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (384.19, 172.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675516 sequences (100000059 bp) in 2.190 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279961, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (385.12, 173.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675840 reads in 175.125 CPU sec, 5.839 real sec
[M::mem_process_seqs] Processed 675854 reads in 110.129 CPU sec, 3.762 real sec
[M::process] read 675220 sequences (100000121 bp) in 2.096 CPU sec, 2.357 real sec...
sorting block 21, #reads = 1066701 mem = 536879626 peak = 865959936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280509, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (387.71, 172.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675318 sequences (100000132 bp) in 2.522 CPU sec, 2.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280684, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (389.11, 172.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675516 reads in 176.939 CPU sec, 5.972 real sec
[M::mem_process_seqs] Processed 675220 reads in 109.500 CPU sec, 3.745 real sec
sorting block 22, #reads = 1066150 mem = 536877201 peak = 866983936
[M::process] read 675216 sequences (100000061 bp) in 2.246 CPU sec, 2.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280658, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 381, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (390.26, 173.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 23, #reads = 1066204 mem = 536879270 peak = 884707328
[M::process] read 674974 sequences (100000069 bp) in 2.192 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280868, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (390.42, 172.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675318 reads in 172.683 CPU sec, 5.897 real sec
[M::mem_process_seqs] Processed 675216 reads in 108.173 CPU sec, 3.831 real sec
[M::process] read 674668 sequences (100000230 bp) in 2.113 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279977, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (392.27, 173.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1065340 mem = 536878760 peak = 884707328
[M::process] read 674510 sequences (100000099 bp) in 2.200 CPU sec, 2.774 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280139, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (268, 453, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (349.80, 109.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (393.19, 173.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674974 reads in 175.475 CPU sec, 5.977 real sec
[M::process] read 674608 sequences (100000151 bp) in 1.780 CPU sec, 1.993 real sec...
sorting block 25, #reads = 1065971 mem = 536882049 peak = 884707328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 279921, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (396.03, 175.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674510 reads in 98.093 CPU sec, 3.253 real sec
[M::process] read 674626 sequences (100000196 bp) in 1.952 CPU sec, 1.970 real sec...
[M::mem_process_seqs] Processed 674668 reads in 261.343 CPU sec, 8.908 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280405, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (396.49, 175.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1066257 mem = 536878161 peak = 884707328
[M::process] read 674998 sequences (100000279 bp) in 2.487 CPU sec, 3.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280544, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (394.43, 175.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674608 reads in 190.160 CPU sec, 6.407 real sec
[M::mem_process_seqs] Processed 674626 reads in 111.030 CPU sec, 3.773 real sec
[M::process] read 674992 sequences (100000297 bp) in 2.097 CPU sec, 2.362 real sec...
sorting block 27, #reads = 1065350 mem = 536876551 peak = 884707328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281265, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (390.83, 173.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674942 sequences (100000209 bp) in 2.137 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280099, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 274, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1765)
[M::mem_pestat] mean and std.dev: (362.90, 260.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2310)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (390.08, 173.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674998 reads in 176.999 CPU sec, 5.921 real sec
[M::process] read 675122 sequences (100000160 bp) in 2.021 CPU sec, 2.240 real sec...
sorting block 28, #reads = 1066195 mem = 536878551 peak = 884707328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280911, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (391.33, 174.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674942 reads in 99.558 CPU sec, 3.328 real sec
[M::process] read 675142 sequences (100000155 bp) in 2.524 CPU sec, 3.183 real sec...
[M::mem_process_seqs] Processed 674992 reads in 285.345 CPU sec, 9.479 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280423, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (390.50, 174.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1065607 mem = 536877106 peak = 884707328
[M::process] read 674952 sequences (100000149 bp) in 2.255 CPU sec, 2.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280917, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (388.92, 173.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675122 reads in 190.509 CPU sec, 6.273 real sec
[M::mem_process_seqs] Processed 675142 reads in 114.073 CPU sec, 3.663 real sec
sorting block 30, #reads = 1065523 mem = 536878992 peak = 884707328
[M::process] read 675074 sequences (100000002 bp) in 2.277 CPU sec, 2.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280643, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (388.62, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674844 sequences (100000037 bp) in 2.359 CPU sec, 2.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280431, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (389.05, 173.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674952 reads in 174.458 CPU sec, 5.801 real sec
[M::mem_process_seqs] Processed 675074 reads in 108.589 CPU sec, 3.583 real sec
sorting block 31, #reads = 1065136 mem = 536879027 peak = 910000128
[M::process] read 674840 sequences (100000063 bp) in 2.538 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280655, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (389.32, 172.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 32, #reads = 1065331 mem = 536880882 peak = 910000128
[M::process] read 674614 sequences (100000271 bp) in 2.226 CPU sec, 2.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280250, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 377, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (388.72, 172.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674844 reads in 177.817 CPU sec, 5.892 real sec
[M::mem_process_seqs] Processed 674840 reads in 108.918 CPU sec, 3.660 real sec
[M::process] read 674678 sequences (100000140 bp) in 2.012 CPU sec, 2.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279880, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (392.53, 173.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1065647 mem = 536878224 peak = 910000128
[M::process] read 674422 sequences (100000117 bp) in 2.359 CPU sec, 2.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281046, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (391.72, 173.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674614 reads in 180.990 CPU sec, 6.037 real sec
[M::mem_process_seqs] Processed 674678 reads in 109.656 CPU sec, 3.694 real sec
[M::process] read 674518 sequences (100000122 bp) in 1.816 CPU sec, 1.911 real sec...
sorting block 34, #reads = 1065335 mem = 536878772 peak = 910000128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280914, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (393.81, 174.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674236 sequences (100000149 bp) in 2.534 CPU sec, 2.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280412, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (392.18, 173.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674422 reads in 175.659 CPU sec, 5.693 real sec
[M::mem_process_seqs] Processed 674518 reads in 111.614 CPU sec, 3.573 real sec
[M::process] read 674328 sequences (100000094 bp) in 2.036 CPU sec, 2.122 real sec...
sorting block 35, #reads = 1065183 mem = 536884058 peak = 910000128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280689, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (392.18, 172.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673856 sequences (100000168 bp) in 2.669 CPU sec, 3.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280923, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (393.85, 173.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674236 reads in 174.834 CPU sec, 5.672 real sec
sorting block 36, #reads = 1064449 mem = 536877293 peak = 920068096
[M::process] read 673728 sequences (100000268 bp) in 1.942 CPU sec, 1.973 real sec...
[M::mem_process_seqs] Processed 674328 reads in 110.531 CPU sec, 3.530 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280838, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (396.98, 174.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 259, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (249.11, 54.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 688)
[M::mem_pestat] skip orientation RR
sorting block 37, #reads = 1064240 mem = 536877528 peak = 982855680
[M::process] read 674578 sequences (100000127 bp) in 2.379 CPU sec, 2.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280524, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 392, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (401.48, 177.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673856 reads in 169.505 CPU sec, 5.620 real sec
[M::mem_process_seqs] Processed 673728 reads in 110.227 CPU sec, 3.647 real sec
[M::process] read 677462 sequences (100000164 bp) in 2.067 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281314, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (397.50, 177.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1064651 mem = 536879118 peak = 982855680
[M::process] read 676670 sequences (100000116 bp) in 2.388 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281913, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 359, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (370.13, 166.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674578 reads in 174.475 CPU sec, 5.818 real sec
[M::mem_process_seqs] Processed 677462 reads in 114.234 CPU sec, 3.833 real sec
[M::process] read 676412 sequences (100000127 bp) in 2.263 CPU sec, 2.511 real sec...
sorting block 39, #reads = 1064699 mem = 536877634 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281324, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 363, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (373.41, 166.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676446 sequences (100000159 bp) in 2.039 CPU sec, 2.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280787, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 364, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (374.46, 166.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676670 reads in 177.319 CPU sec, 5.988 real sec
[M::mem_process_seqs] Processed 676412 reads in 113.124 CPU sec, 3.824 real sec
sorting block 40, #reads = 1066493 mem = 536877351 peak = 982855680
[M::process] read 675716 sequences (100000174 bp) in 2.256 CPU sec, 2.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280789, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 369, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (378.10, 168.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675812 sequences (100000224 bp) in 2.574 CPU sec, 3.089 real sec...
sorting block 41, #reads = 1066047 mem = 536879924 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280573, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 371, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (379.66, 167.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676446 reads in 180.012 CPU sec, 5.953 real sec
[M::mem_process_seqs] Processed 675716 reads in 114.783 CPU sec, 3.913 real sec
[M::process] read 675338 sequences (100000218 bp) in 2.015 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280990, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 372, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (380.59, 167.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1065852 mem = 536881832 peak = 982855680
[M::process] read 675330 sequences (100000048 bp) in 2.287 CPU sec, 2.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281025, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 374, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (381.44, 166.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675812 reads in 173.718 CPU sec, 5.896 real sec
[M::mem_process_seqs] Processed 675338 reads in 109.616 CPU sec, 3.833 real sec
[M::process] read 674914 sequences (100000027 bp) in 1.954 CPU sec, 2.199 real sec...
sorting block 43, #reads = 1065582 mem = 536883026 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280741, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 374, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (382.50, 166.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674840 sequences (100000043 bp) in 2.471 CPU sec, 2.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280276, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 379, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (386.77, 168.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675330 reads in 177.741 CPU sec, 6.022 real sec
[M::mem_process_seqs] Processed 674914 reads in 111.366 CPU sec, 3.873 real sec
[M::process] read 674696 sequences (100000038 bp) in 1.870 CPU sec, 2.014 real sec...
sorting block 44, #reads = 1064442 mem = 536883382 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279541, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 379, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (386.97, 169.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674384 sequences (100000298 bp) in 2.335 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279910, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (389.40, 169.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674840 reads in 182.834 CPU sec, 6.136 real sec
[M::mem_process_seqs] Processed 674696 reads in 113.124 CPU sec, 3.852 real sec
sorting block 45, #reads = 1064925 mem = 536876820 peak = 982855680
[M::process] read 674726 sequences (100000142 bp) in 1.965 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280663, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (391.84, 170.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1064498 mem = 536879986 peak = 982855680
[M::process] read 674576 sequences (100000155 bp) in 2.923 CPU sec, 3.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 279913, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (389.46, 169.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674384 reads in 187.439 CPU sec, 6.159 real sec
[M::mem_process_seqs] Processed 674726 reads in 120.415 CPU sec, 3.935 real sec
[M::process] read 674770 sequences (100000100 bp) in 1.816 CPU sec, 1.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279753, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (392.05, 171.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1064202 mem = 536879634 peak = 982855680
[M::process] read 674712 sequences (100000185 bp) in 2.567 CPU sec, 3.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280407, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 381, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (389.23, 170.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674576 reads in 185.303 CPU sec, 6.145 real sec
[M::mem_process_seqs] Processed 674770 reads in 119.339 CPU sec, 3.926 real sec
[M::process] read 674872 sequences (100000065 bp) in 1.766 CPU sec, 1.847 real sec...
sorting block 48, #reads = 1064771 mem = 536879986 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280003, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (388.09, 170.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674884 sequences (100000093 bp) in 2.878 CPU sec, 3.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280446, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (387.47, 170.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674712 reads in 179.605 CPU sec, 5.760 real sec
[M::mem_process_seqs] Processed 674872 reads in 110.919 CPU sec, 3.494 real sec
[M::process] read 674812 sequences (100000263 bp) in 2.176 CPU sec, 2.219 real sec...
sorting block 49, #reads = 1064162 mem = 536877953 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280207, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (388.86, 171.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674852 sequences (100000133 bp) in 2.430 CPU sec, 2.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280143, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (387.89, 171.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674566 sequences (100000081 bp) in 2.011 CPU sec, 2.025 real sec...
[M::mem_process_seqs] Processed 674884 reads in 177.328 CPU sec, 5.690 real sec
sorting block 50, #reads = 1064958 mem = 536880851 peak = 982855680
[M::mem_process_seqs] Processed 674812 reads in 114.720 CPU sec, 3.711 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279723, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (386.54, 170.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1064946 mem = 536881396 peak = 982855680
[M::process] read 674630 sequences (100000218 bp) in 2.858 CPU sec, 3.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279901, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (389.34, 171.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674852 reads in 188.448 CPU sec, 6.201 real sec
[M::process] read 674558 sequences (100000212 bp) in 1.935 CPU sec, 1.948 real sec...
[M::mem_process_seqs] Processed 674566 reads in 116.804 CPU sec, 3.704 real sec
sorting block 52, #reads = 1063869 mem = 536878782 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279426, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (390.05, 171.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 319, 906)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2274)
[M::mem_pestat] mean and std.dev: (491.60, 341.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2958)
[M::mem_pestat] skip orientation RR
[M::process] read 674496 sequences (100000245 bp) in 2.585 CPU sec, 2.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279629, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (391.06, 172.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674630 reads in 163.900 CPU sec, 5.231 real sec
[M::mem_process_seqs] Processed 674558 reads in 93.829 CPU sec, 2.954 real sec
[M::process] read 674474 sequences (100000104 bp) in 1.831 CPU sec, 1.842 real sec...
sorting block 53, #reads = 1064316 mem = 536877653 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280433, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (391.63, 172.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674406 sequences (100000265 bp) in 2.778 CPU sec, 2.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279786, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (392.45, 172.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674496 reads in 167.009 CPU sec, 5.303 real sec
[M::process] read 674244 sequences (100000288 bp) in 1.925 CPU sec, 1.923 real sec...
[M::mem_process_seqs] Processed 674474 reads in 107.592 CPU sec, 3.396 real sec
sorting block 54, #reads = 1064932 mem = 536878110 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279573, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (393.43, 172.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1065652 mem = 536879407 peak = 982855680
[M::process] read 674028 sequences (100000116 bp) in 2.403 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 279986, 5, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 166, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (207.92, 74.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 554)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (395.29, 173.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 673666 sequences (100000268 bp) in 1.778 CPU sec, 1.784 real sec...
[M::mem_process_seqs] Processed 674406 reads in 174.089 CPU sec, 5.560 real sec
[M::mem_process_seqs] Processed 674244 reads in 107.977 CPU sec, 3.414 real sec
sorting block 56, #reads = 1065050 mem = 536880488 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 280110, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 432, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (349.00, 174.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 388, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (397.52, 174.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 673766 sequences (100000039 bp) in 2.615 CPU sec, 2.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280701, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 390, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (399.46, 174.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673690 sequences (100000299 bp) in 1.820 CPU sec, 1.815 real sec...
[M::mem_process_seqs] Processed 674028 reads in 162.917 CPU sec, 5.174 real sec
[M::mem_process_seqs] Processed 673666 reads in 100.384 CPU sec, 3.168 real sec
sorting block 57, #reads = 1064594 mem = 536881164 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280326, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 388, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (397.74, 174.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677046 sequences (100000163 bp) in 2.350 CPU sec, 2.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280568, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 255, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346)
[M::mem_pestat] mean and std.dev: (324.09, 194.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1752)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 390, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (399.64, 174.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 673766 reads in 158.810 CPU sec, 5.060 real sec
[M::process] read 676744 sequences (100000126 bp) in 1.882 CPU sec, 1.903 real sec...
[M::mem_process_seqs] Processed 673690 reads in 101.496 CPU sec, 3.192 real sec
sorting block 58, #reads = 1065470 mem = 536879684 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280265, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 363, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (375.86, 171.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676586 sequences (100000026 bp) in 2.362 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 280346, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (355, 481, 696)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378)
[M::mem_pestat] mean and std.dev: (448.17, 176.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1719)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 368, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (380.17, 173.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675946 sequences (100000170 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 677046 reads in 156.739 CPU sec, 5.011 real sec
sorting block 59, #reads = 1064605 mem = 536879535 peak = 982855680
[M::mem_process_seqs] Processed 676744 reads in 92.274 CPU sec, 2.927 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 280534, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (290, 1131, 1131)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2813)
[M::mem_pestat] mean and std.dev: (791.54, 453.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 371, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (382.44, 173.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 60, #reads = 1067247 mem = 536879475 peak = 982855680
[M::process] read 676066 sequences (100000036 bp) in 2.324 CPU sec, 2.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280352, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 375, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (384.84, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675506 sequences (100000001 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_process_seqs] Processed 676586 reads in 139.747 CPU sec, 4.436 real sec
[M::mem_process_seqs] Processed 675946 reads in 84.984 CPU sec, 2.681 real sec
sorting block 61, #reads = 1067153 mem = 536882424 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280206, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 375, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (385.99, 173.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675464 sequences (100000218 bp) in 2.489 CPU sec, 2.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280797, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (388.10, 173.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675408 sequences (100000157 bp) in 1.379 CPU sec, 1.384 real sec...
[M::mem_process_seqs] Processed 676066 reads in 144.559 CPU sec, 4.590 real sec
[M::mem_process_seqs] Processed 675506 reads in 90.883 CPU sec, 2.871 real sec
sorting block 62, #reads = 1067053 mem = 536881668 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279896, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (389.65, 174.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675344 sequences (100000011 bp) in 2.230 CPU sec, 2.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279520, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (389.85, 173.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675236 sequences (100000102 bp) in 1.750 CPU sec, 1.755 real sec...
[M::mem_process_seqs] Processed 675464 reads in 157.577 CPU sec, 5.010 real sec
[M::mem_process_seqs] Processed 675408 reads in 99.281 CPU sec, 3.134 real sec
sorting block 63, #reads = 1066059 mem = 536878343 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279840, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 381, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (389.47, 172.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675666 sequences (100000183 bp) in 2.525 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279679, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (388.75, 171.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675344 reads in 157.371 CPU sec, 4.994 real sec
[M::process] read 674786 sequences (100000158 bp) in 1.800 CPU sec, 1.804 real sec...
sorting block 64, #reads = 1065910 mem = 536878805 peak = 982855680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280220, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674644 sequences (100000124 bp) in 1.631 CPU sec, 1.640 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (249, 375, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (385.57, 172.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675666 reads in 81.399 CPU sec, 2.581 real sec
sorting block 65, #reads = 1066484 mem = 536879158 peak = 997847040
[M::mem_process_seqs] Processed 675236 reads in 200.306 CPU sec, 6.336 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279994, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (392.24, 171.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674904 sequences (100000204 bp) in 2.782 CPU sec, 2.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279236, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (392.14, 171.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674786 reads in 181.511 CPU sec, 5.759 real sec
[M::process] read 674806 sequences (100000221 bp) in 1.861 CPU sec, 1.884 real sec...
sorting block 66, #reads = 1065741 mem = 536880296 peak = 997847040
[M::mem_process_seqs] Processed 674644 reads in 110.547 CPU sec, 3.485 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280141, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (391.20, 172.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 224, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 467)
[M::mem_pestat] mean and std.dev: (228.11, 38.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 550)
[M::mem_pestat] skip orientation RR
sorting block 67, #reads = 1065457 mem = 536879553 peak = 1007616000
[M::process] read 674842 sequences (100000119 bp) in 2.728 CPU sec, 2.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279835, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (389.88, 171.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675030 sequences (100000023 bp) in 1.837 CPU sec, 1.839 real sec...
[M::mem_process_seqs] Processed 674904 reads in 178.589 CPU sec, 5.688 real sec
[M::mem_process_seqs] Processed 674806 reads in 110.633 CPU sec, 3.498 real sec
sorting block 68, #reads = 1064367 mem = 536878214 peak = 1007616000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279711, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (388.35, 170.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674804 sequences (100000137 bp) in 2.840 CPU sec, 2.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 279753, 5, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (273, 299, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1464)
[M::mem_pestat] mean and std.dev: (433.59, 293.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1861)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (386.89, 170.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674892 sequences (100000170 bp) in 1.901 CPU sec, 1.907 real sec...
[M::mem_process_seqs] Processed 674842 reads in 176.779 CPU sec, 5.617 real sec
[M::mem_process_seqs] Processed 675030 reads in 110.019 CPU sec, 3.478 real sec
sorting block 69, #reads = 1065433 mem = 536877695 peak = 1007616000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280072, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (388.75, 171.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674984 sequences (100000076 bp) in 2.847 CPU sec, 2.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279536, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (388.21, 170.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674930 sequences (100000052 bp) in 1.866 CPU sec, 1.873 real sec...
[M::mem_process_seqs] Processed 674804 reads in 177.716 CPU sec, 5.655 real sec
[M::mem_process_seqs] Processed 674892 reads in 111.654 CPU sec, 3.523 real sec
sorting block 70, #reads = 1065410 mem = 536880470 peak = 1007616000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279575, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (386.31, 169.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674924 sequences (100000050 bp) in 2.796 CPU sec, 2.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279657, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (386.51, 169.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674776 sequences (100000035 bp) in 1.844 CPU sec, 1.845 real sec...
[M::mem_process_seqs] Processed 674984 reads in 179.071 CPU sec, 5.699 real sec
sorting block 71, #reads = 1064861 mem = 536878657 peak = 1007616000
[M::mem_process_seqs] Processed 674930 reads in 110.657 CPU sec, 3.501 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280192, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (387.42, 170.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 72, #reads = 1064943 mem = 536879541 peak = 1068068864
[M::process] read 674524 sequences (100000043 bp) in 2.893 CPU sec, 3.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280197, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 561, 561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173)
[M::mem_pestat] mean and std.dev: (406.00, 174.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (385.57, 167.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674626 sequences (100000160 bp) in 1.823 CPU sec, 1.834 real sec...
[M::mem_process_seqs] Processed 674924 reads in 176.272 CPU sec, 5.606 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279989, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (387.94, 169.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674456 sequences (100000166 bp) in 2.172 CPU sec, 2.173 real sec...
[M::mem_process_seqs] Processed 674524 reads in 101.199 CPU sec, 3.410 real sec
sorting block 73, #reads = 1064338 mem = 536881329 peak = 1068068864
[M::mem_process_seqs] Processed 674776 reads in 242.874 CPU sec, 7.909 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279360, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (388.07, 169.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1064363 mem = 536877106 peak = 1068068864
[M::process] read 674426 sequences (100000043 bp) in 2.595 CPU sec, 2.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280144, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (385.72, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674626 reads in 187.898 CPU sec, 5.970 real sec
[M::mem_process_seqs] Processed 674456 reads in 107.638 CPU sec, 3.389 real sec
[M::process] read 674228 sequences (100000250 bp) in 1.956 CPU sec, 1.992 real sec...
sorting block 75, #reads = 1064978 mem = 536880873 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280220, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 377, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (386.41, 168.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673954 sequences (100000267 bp) in 2.386 CPU sec, 2.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280539, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (390.03, 169.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673962 sequences (100000197 bp) in 1.849 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 674426 reads in 170.012 CPU sec, 5.416 real sec
[M::mem_process_seqs] Processed 674228 reads in 105.337 CPU sec, 3.329 real sec
sorting block 76, #reads = 1064411 mem = 536879026 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280185, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 385, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (393.79, 170.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 311, 747)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1865)
[M::mem_pestat] mean and std.dev: (437.80, 255.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2424)
[M::mem_pestat] skip orientation RR
[M::process] read 673680 sequences (100000100 bp) in 2.737 CPU sec, 2.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280152, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 388, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (397.28, 173.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673954 reads in 165.717 CPU sec, 5.278 real sec
[M::process] read 678894 sequences (100000133 bp) in 1.965 CPU sec, 1.975 real sec...
[M::mem_process_seqs] Processed 673962 reads in 103.168 CPU sec, 3.257 real sec
sorting block 77, #reads = 1064815 mem = 536878423 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280148, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 391, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (400.13, 175.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678644 sequences (100000267 bp) in 2.526 CPU sec, 2.672 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282376, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 352, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (368.27, 174.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677644 sequences (100000191 bp) in 1.874 CPU sec, 1.879 real sec...
[M::mem_process_seqs] Processed 673680 reads in 171.703 CPU sec, 5.466 real sec
sorting block 78, #reads = 1064462 mem = 536880313 peak = 1068068864
[M::mem_process_seqs] Processed 678894 reads in 112.481 CPU sec, 3.562 real sec
sorting block 79, #reads = 1063997 mem = 536878452 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281621, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 357, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (372.69, 176.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677376 sequences (100000052 bp) in 2.815 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281857, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 362, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (376.41, 175.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 512, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (388.58, 155.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RR
[M::process] read 677254 sequences (100000117 bp) in 1.913 CPU sec, 1.916 real sec...
[M::mem_process_seqs] Processed 678644 reads in 177.802 CPU sec, 5.643 real sec
[M::mem_process_seqs] Processed 677644 reads in 111.357 CPU sec, 3.521 real sec
sorting block 80, #reads = 1066201 mem = 536877666 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281330, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 366, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (378.21, 174.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676702 sequences (100000001 bp) in 2.403 CPU sec, 2.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281518, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 368, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (379.42, 174.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676676 sequences (100000074 bp) in 1.810 CPU sec, 1.817 real sec...
[M::mem_process_seqs] Processed 677376 reads in 181.840 CPU sec, 5.817 real sec
[M::mem_process_seqs] Processed 677254 reads in 114.558 CPU sec, 3.639 real sec
sorting block 81, #reads = 1065251 mem = 536877670 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281102, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 374, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (383.92, 175.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676234 sequences (100000264 bp) in 2.901 CPU sec, 3.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280921, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 374, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (384.64, 175.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675880 sequences (100000288 bp) in 1.908 CPU sec, 1.924 real sec...
[M::mem_process_seqs] Processed 676702 reads in 183.868 CPU sec, 5.867 real sec
sorting block 82, #reads = 1063815 mem = 536879011 peak = 1068068864
[M::mem_process_seqs] Processed 676676 reads in 120.668 CPU sec, 3.828 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281474, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 379, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (388.38, 176.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 83, #reads = 1063967 mem = 536880877 peak = 1068068864
[M::process] read 675746 sequences (100000237 bp) in 3.093 CPU sec, 3.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281149, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (390.13, 176.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676234 reads in 181.442 CPU sec, 5.886 real sec
[M::mem_process_seqs] Processed 675880 reads in 116.471 CPU sec, 3.821 real sec
[M::process] read 675532 sequences (100000022 bp) in 1.962 CPU sec, 2.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280802, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (390.93, 175.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 84, #reads = 1064037 mem = 536879938 peak = 1068068864
[M::process] read 675698 sequences (100000051 bp) in 2.608 CPU sec, 3.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281424, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (391.62, 175.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675746 reads in 182.880 CPU sec, 6.075 real sec
[M::mem_process_seqs] Processed 675532 reads in 117.450 CPU sec, 3.832 real sec
[M::process] read 675512 sequences (100000011 bp) in 2.209 CPU sec, 2.458 real sec...
sorting block 85, #reads = 1063991 mem = 536878576 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280997, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (391.29, 174.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675176 sequences (100000020 bp) in 2.452 CPU sec, 2.728 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281081, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (393.05, 175.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675698 reads in 186.597 CPU sec, 6.051 real sec
[M::mem_process_seqs] Processed 675512 reads in 118.935 CPU sec, 3.807 real sec
[M::process] read 675180 sequences (100000187 bp) in 2.219 CPU sec, 2.316 real sec...
sorting block 86, #reads = 1062437 mem = 536879527 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280697, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 385, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (393.64, 175.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675220 sequences (100000261 bp) in 2.585 CPU sec, 2.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280326, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (391.22, 173.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675180 sequences (100000188 bp) in 1.751 CPU sec, 1.757 real sec...
[M::mem_process_seqs] Processed 675176 reads in 189.998 CPU sec, 6.080 real sec
sorting block 87, #reads = 1062145 mem = 536881819 peak = 1068068864
[M::mem_process_seqs] Processed 675180 reads in 118.703 CPU sec, 3.774 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280777, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (390.98, 173.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 88, #reads = 1062330 mem = 536878307 peak = 1068068864
[M::process] read 675076 sequences (100000083 bp) in 2.659 CPU sec, 2.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280551, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (391.98, 173.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675052 sequences (100000082 bp) in 1.891 CPU sec, 1.897 real sec...
[M::mem_process_seqs] Processed 675220 reads in 190.228 CPU sec, 6.077 real sec
[M::mem_process_seqs] Processed 675180 reads in 117.486 CPU sec, 3.735 real sec
sorting block 89, #reads = 1062756 mem = 536881042 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280499, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (390.93, 173.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675200 sequences (100000040 bp) in 2.749 CPU sec, 2.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280764, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (390.31, 172.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674964 sequences (100000117 bp) in 1.909 CPU sec, 1.911 real sec...
[M::mem_process_seqs] Processed 675076 reads in 185.969 CPU sec, 5.933 real sec
[M::mem_process_seqs] Processed 675052 reads in 117.173 CPU sec, 3.721 real sec
sorting block 90, #reads = 1062554 mem = 536880326 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280646, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (389.45, 172.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675058 sequences (100000043 bp) in 2.603 CPU sec, 2.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281090, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (390.52, 172.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 546, 899)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2227)
[M::mem_pestat] mean and std.dev: (564.10, 298.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2891)
[M::mem_pestat] skip orientation RR
[M::process] read 675034 sequences (100000221 bp) in 1.910 CPU sec, 1.915 real sec...
[M::mem_process_seqs] Processed 675200 reads in 191.619 CPU sec, 6.130 real sec
[M::mem_process_seqs] Processed 674964 reads in 121.689 CPU sec, 3.867 real sec
sorting block 91, #reads = 1062143 mem = 536880283 peak = 1068068864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281132, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 381, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (388.58, 170.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675120 sequences (100000127 bp) in 2.930 CPU sec, 3.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280428, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (386.64, 170.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675114 sequences (100000133 bp) in 1.941 CPU sec, 1.945 real sec...
[M::mem_process_seqs] Processed 675058 reads in 190.476 CPU sec, 6.068 real sec
sorting block 92, #reads = 1061760 mem = 536876669 peak = 1068068864
[M::mem_process_seqs] Processed 675034 reads in 119.002 CPU sec, 3.776 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281256, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (384.67, 168.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 93, #reads = 1061736 mem = 536886337 peak = 1069322240
[M::process] read 674992 sequences (100000193 bp) in 2.588 CPU sec, 2.811 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280821, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (383.72, 168.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674984 sequences (100000040 bp) in 1.951 CPU sec, 1.954 real sec...
[M::mem_process_seqs] Processed 675120 reads in 186.204 CPU sec, 5.953 real sec
[M::mem_process_seqs] Processed 675114 reads in 117.808 CPU sec, 3.738 real sec
sorting block 94, #reads = 1063040 mem = 536881854 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280369, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (386.63, 170.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674980 sequences (100000153 bp) in 2.686 CPU sec, 2.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281137, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (388.81, 172.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674994 sequences (100000178 bp) in 1.792 CPU sec, 1.793 real sec...
[M::mem_process_seqs] Processed 674992 reads in 186.139 CPU sec, 5.957 real sec
[M::mem_process_seqs] Processed 674984 reads in 118.784 CPU sec, 3.786 real sec
sorting block 95, #reads = 1062643 mem = 536878052 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281840, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (391.00, 173.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679968 sequences (100000182 bp) in 2.622 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281651, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (395.40, 177.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679630 sequences (100000236 bp) in 1.989 CPU sec, 1.992 real sec...
[M::mem_process_seqs] Processed 674980 reads in 184.010 CPU sec, 5.894 real sec
sorting block 96, #reads = 1062130 mem = 536878943 peak = 1069322240
[M::mem_process_seqs] Processed 674994 reads in 118.473 CPU sec, 3.754 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283282, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (360.60, 171.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 97, #reads = 1062320 mem = 536877068 peak = 1069322240
[M::process] read 678536 sequences (100000270 bp) in 2.932 CPU sec, 3.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283661, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 337, 623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437)
[M::mem_pestat] mean and std.dev: (375.18, 180.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1844)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (360.10, 169.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 678330 sequences (100000052 bp) in 1.931 CPU sec, 1.934 real sec...
[M::mem_process_seqs] Processed 679968 reads in 192.920 CPU sec, 6.172 real sec
[M::mem_process_seqs] Processed 679630 reads in 122.376 CPU sec, 3.890 real sec
sorting block 98, #reads = 1062207 mem = 536880947 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282803, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 351, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (364.67, 168.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678098 sequences (100000152 bp) in 2.655 CPU sec, 2.866 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282801, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 357, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (369.29, 170.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678150 sequences (100000164 bp) in 1.988 CPU sec, 1.995 real sec...
[M::mem_process_seqs] Processed 678536 reads in 186.445 CPU sec, 5.969 real sec
[M::mem_process_seqs] Processed 678330 reads in 119.133 CPU sec, 3.788 real sec
sorting block 99, #reads = 1062826 mem = 536876429 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282228, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 359, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (371.38, 170.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678022 sequences (100000171 bp) in 2.936 CPU sec, 3.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282838, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 354, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (366.62, 167.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677354 sequences (100000236 bp) in 2.015 CPU sec, 2.018 real sec...
[M::mem_process_seqs] Processed 678098 reads in 189.713 CPU sec, 6.065 real sec
[M::mem_process_seqs] Processed 678150 reads in 124.411 CPU sec, 3.940 real sec
sorting block 100, #reads = 1062888 mem = 536878094 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283510, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 359, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (370.43, 169.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676804 sequences (100000035 bp) in 2.524 CPU sec, 2.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282573, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 366, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (377.04, 172.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676738 sequences (100000167 bp) in 1.918 CPU sec, 1.928 real sec...
[M::mem_process_seqs] Processed 678022 reads in 185.612 CPU sec, 5.940 real sec
sorting block 101, #reads = 1060165 mem = 536880875 peak = 1069322240
[M::mem_process_seqs] Processed 677354 reads in 113.959 CPU sec, 3.601 real sec
sorting block 102, #reads = 1060790 mem = 536880691 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282051, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 370, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (379.65, 171.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676650 sequences (100000254 bp) in 2.796 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281652, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 373, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (381.53, 171.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676292 sequences (100000081 bp) in 1.850 CPU sec, 1.857 real sec...
[M::mem_process_seqs] Processed 676804 reads in 174.139 CPU sec, 5.528 real sec
[M::mem_process_seqs] Processed 676738 reads in 109.506 CPU sec, 3.462 real sec
sorting block 103, #reads = 1061528 mem = 536878538 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281663, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 374, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (382.42, 172.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676092 sequences (100000017 bp) in 2.514 CPU sec, 2.632 real sec...
[M::process] read 675666 sequences (100000254 bp) in 1.750 CPU sec, 1.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281797, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 377, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (385.93, 173.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676650 reads in 178.951 CPU sec, 5.711 real sec
[M::mem_process_seqs] Processed 676292 reads in 114.844 CPU sec, 3.645 real sec
sorting block 104, #reads = 1061934 mem = 536878529 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282113, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (386.48, 172.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675578 sequences (100000078 bp) in 2.478 CPU sec, 2.593 real sec...
[M::process] read 675434 sequences (100000081 bp) in 1.839 CPU sec, 1.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281255, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (389.18, 173.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676092 reads in 185.509 CPU sec, 5.931 real sec
sorting block 105, #reads = 1060976 mem = 536880252 peak = 1069322240
[M::mem_process_seqs] Processed 675666 reads in 115.856 CPU sec, 3.689 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281531, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (390.03, 174.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 106, #reads = 1061177 mem = 536878858 peak = 1069322240
[M::process] read 675496 sequences (100000103 bp) in 2.624 CPU sec, 2.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281227, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (389.98, 174.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675194 sequences (100000006 bp) in 2.046 CPU sec, 2.048 real sec...
[M::mem_process_seqs] Processed 675578 reads in 182.719 CPU sec, 5.808 real sec
[M::mem_process_seqs] Processed 675434 reads in 112.312 CPU sec, 3.545 real sec
sorting block 107, #reads = 1061247 mem = 536879193 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280958, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (390.65, 174.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675074 sequences (100000049 bp) in 2.666 CPU sec, 2.794 real sec...
[M::process] read 675348 sequences (100000178 bp) in 1.480 CPU sec, 1.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280928, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (393.16, 175.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675496 reads in 176.985 CPU sec, 5.648 real sec
[M::mem_process_seqs] Processed 675194 reads in 110.194 CPU sec, 3.499 real sec
sorting block 108, #reads = 1061825 mem = 536882848 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280853, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (391.82, 174.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675014 sequences (100000011 bp) in 2.699 CPU sec, 2.811 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281065, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (392.78, 174.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675074 reads in 170.133 CPU sec, 5.430 real sec
[M::mem_process_seqs] Processed 675348 reads in 106.100 CPU sec, 3.336 real sec
[M::process] read 674856 sequences (100000234 bp) in 2.236 CPU sec, 2.267 real sec...
sorting block 109, #reads = 1061963 mem = 536880844 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281190, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 386, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (395.81, 177.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 618, 851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2113)
[M::mem_pestat] mean and std.dev: (610.60, 327.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2744)
[M::mem_pestat] skip orientation RR
[M::process] read 674894 sequences (100000282 bp) in 2.245 CPU sec, 2.372 real sec...
[M::process] read 674644 sequences (100000255 bp) in 1.480 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281264, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 387, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (396.57, 177.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1320)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675014 reads in 169.465 CPU sec, 5.411 real sec
sorting block 110, #reads = 1062254 mem = 536881080 peak = 1069322240
[M::mem_process_seqs] Processed 674856 reads in 105.607 CPU sec, 3.355 real sec
sorting block 111, #reads = 1062844 mem = 536878461 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281317, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 390, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064)
[M::mem_pestat] mean and std.dev: (399.73, 179.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1333)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674396 sequences (100000112 bp) in 2.615 CPU sec, 2.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280901, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 394, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (403.58, 181.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1347)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674506 sequences (100000239 bp) in 1.833 CPU sec, 1.842 real sec...
[M::mem_process_seqs] Processed 674894 reads in 169.276 CPU sec, 5.373 real sec
[M::mem_process_seqs] Processed 674644 reads in 106.727 CPU sec, 3.364 real sec
sorting block 112, #reads = 1063178 mem = 536879765 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280777, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 394, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079)
[M::mem_pestat] mean and std.dev: (404.87, 183.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1352)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675022 sequences (100000166 bp) in 2.520 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280903, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 396, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (407.04, 185.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1364)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678686 sequences (100000102 bp) in 1.862 CPU sec, 1.864 real sec...
[M::mem_process_seqs] Processed 674396 reads in 171.853 CPU sec, 5.460 real sec
[M::mem_process_seqs] Processed 674506 reads in 109.634 CPU sec, 3.456 real sec
sorting block 113, #reads = 1061440 mem = 536878333 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281283, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 393, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (405.07, 187.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1380)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 301, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (300.18, 109.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RR
[M::process] read 678254 sequences (100000133 bp) in 2.524 CPU sec, 2.623 real sec...
[M::process] read 677578 sequences (100000081 bp) in 1.803 CPU sec, 1.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282409, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 353, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (368.30, 173.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675022 reads in 186.151 CPU sec, 5.921 real sec
sorting block 114, #reads = 1059228 mem = 536880757 peak = 1069322240
[M::mem_process_seqs] Processed 678686 reads in 117.988 CPU sec, 3.732 real sec
sorting block 115, #reads = 1055690 mem = 536878494 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281904, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 356, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (370.06, 172.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677156 sequences (100000234 bp) in 2.464 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281420, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 361, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (372.83, 170.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677208 sequences (100000017 bp) in 1.910 CPU sec, 1.913 real sec...
[M::mem_process_seqs] Processed 678254 reads in 168.306 CPU sec, 5.336 real sec
[M::mem_process_seqs] Processed 677578 reads in 100.891 CPU sec, 3.178 real sec
sorting block 116, #reads = 1066037 mem = 536880397 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281952, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 363, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (373.99, 169.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 196, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565)
[M::mem_pestat] mean and std.dev: (269.50, 149.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] skip orientation RR
[M::process] read 676992 sequences (100000046 bp) in 2.108 CPU sec, 2.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281408, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 364, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (374.29, 169.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676916 sequences (100000257 bp) in 1.390 CPU sec, 1.386 real sec...
[M::mem_process_seqs] Processed 677156 reads in 136.575 CPU sec, 4.343 real sec
[M::mem_process_seqs] Processed 677208 reads in 84.124 CPU sec, 2.650 real sec
sorting block 117, #reads = 1063601 mem = 536879976 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281833, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 366, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (375.39, 168.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676158 sequences (100000054 bp) in 2.356 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281897, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 370, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (379.85, 171.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 308, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (261.64, 63.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 679)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676992 reads in 146.589 CPU sec, 4.643 real sec
[M::process] read 675988 sequences (100000266 bp) in 1.902 CPU sec, 1.910 real sec...
[M::mem_process_seqs] Processed 676916 reads in 93.513 CPU sec, 2.936 real sec
sorting block 118, #reads = 1063749 mem = 536887970 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281114, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 375, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (384.26, 172.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676164 sequences (100000168 bp) in 2.517 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280818, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (384.80, 172.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676340 sequences (100000290 bp) in 1.878 CPU sec, 1.875 real sec...
[M::mem_process_seqs] Processed 676158 reads in 162.151 CPU sec, 5.139 real sec
[M::mem_process_seqs] Processed 675988 reads in 102.173 CPU sec, 3.216 real sec
sorting block 119, #reads = 1063253 mem = 536878850 peak = 1069322240
sorting block 120, #reads = 1064065 mem = 536878593 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281026, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 379, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (386.90, 174.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (336, 375, 893)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2007)
[M::mem_pestat] mean and std.dev: (496.91, 266.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2564)
[M::mem_pestat] skip orientation RR
[M::process] read 676280 sequences (100000015 bp) in 2.507 CPU sec, 2.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281206, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (384.87, 171.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676164 reads in 161.618 CPU sec, 5.132 real sec
[M::process] read 675886 sequences (100000010 bp) in 1.810 CPU sec, 1.812 real sec...
[M::mem_process_seqs] Processed 676340 reads in 94.314 CPU sec, 2.970 real sec
sorting block 121, #reads = 1062492 mem = 536879229 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281245, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (383.80, 171.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675594 sequences (100000121 bp) in 2.401 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280499, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (388.70, 173.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676280 reads in 144.226 CPU sec, 4.567 real sec
[M::process] read 675654 sequences (100000134 bp) in 1.850 CPU sec, 1.865 real sec...
[M::mem_process_seqs] Processed 675886 reads in 87.270 CPU sec, 2.737 real sec
sorting block 122, #reads = 1062295 mem = 536880361 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280218, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (389.75, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675246 sequences (100000213 bp) in 2.273 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280700, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (388.71, 171.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675594 reads in 145.779 CPU sec, 4.632 real sec
[M::mem_process_seqs] Processed 675654 reads in 91.857 CPU sec, 2.880 real sec
[M::process] read 675340 sequences (100000015 bp) in 1.914 CPU sec, 1.922 real sec...
sorting block 123, #reads = 1062360 mem = 536878400 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280357, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (389.80, 171.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675036 sequences (100000055 bp) in 2.303 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280461, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (389.71, 171.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675246 reads in 147.262 CPU sec, 4.674 real sec
sorting block 124, #reads = 1060219 mem = 536878895 peak = 1069322240
[M::mem_process_seqs] Processed 675340 reads in 94.259 CPU sec, 2.960 real sec
[M::process] read 675132 sequences (100000163 bp) in 1.961 CPU sec, 1.975 real sec...
sorting block 125, #reads = 1061730 mem = 536879190 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280907, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (389.25, 171.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675310 sequences (100000099 bp) in 2.403 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281168, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (390.70, 173.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675036 reads in 156.576 CPU sec, 4.975 real sec
[M::process] read 675232 sequences (100000083 bp) in 1.640 CPU sec, 1.642 real sec...
[M::mem_process_seqs] Processed 675132 reads in 93.842 CPU sec, 2.953 real sec
sorting block 126, #reads = 1061197 mem = 536877366 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280714, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (388.30, 172.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675226 sequences (100000050 bp) in 2.284 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281416, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (389.54, 173.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675206 sequences (100000292 bp) in 1.389 CPU sec, 1.389 real sec...
[M::mem_process_seqs] Processed 675310 reads in 142.819 CPU sec, 4.532 real sec
[M::mem_process_seqs] Processed 675232 reads in 86.891 CPU sec, 2.738 real sec
sorting block 127, #reads = 1061621 mem = 536880035 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280929, 2, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (388.94, 173.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 409, 2737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7647)
[M::mem_pestat] mean and std.dev: (954.31, 1045.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10102)
[M::mem_pestat] skip orientation RR
[M::process] read 675090 sequences (100000269 bp) in 2.330 CPU sec, 2.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280996, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (388.79, 172.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675134 sequences (100000272 bp) in 1.860 CPU sec, 1.865 real sec...
[M::mem_process_seqs] Processed 675226 reads in 159.428 CPU sec, 5.048 real sec
sorting block 128, #reads = 1060951 mem = 536882066 peak = 1069322240
[M::mem_process_seqs] Processed 675206 reads in 103.028 CPU sec, 3.246 real sec
sorting block 129, #reads = 1059290 mem = 536878876 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280961, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (389.62, 172.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675054 sequences (100000089 bp) in 2.348 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281395, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 675194 sequences (100000142 bp) in 1.890 CPU sec, 1.881 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (252, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (390.91, 174.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675090 reads in 162.760 CPU sec, 5.151 real sec
[M::mem_process_seqs] Processed 675134 reads in 100.051 CPU sec, 3.152 real sec
sorting block 130, #reads = 1060270 mem = 536879910 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280929, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 382, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (391.92, 175.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675294 sequences (100000158 bp) in 2.366 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281072, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 383, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (393.31, 176.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680164 sequences (100000034 bp) in 1.910 CPU sec, 1.909 real sec...
[M::mem_process_seqs] Processed 675054 reads in 162.996 CPU sec, 5.158 real sec
[M::mem_process_seqs] Processed 675194 reads in 101.485 CPU sec, 3.204 real sec
sorting block 131, #reads = 1060214 mem = 536878727 peak = 1069322240
[M::process] read 680664 sequences (100000041 bp) in 2.117 CPU sec, 2.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281215, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 382, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (393.78, 179.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1331)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679824 sequences (100000164 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283538, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 157, 157)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (31, 241)
[M::mem_pestat] mean and std.dev: (140.33, 20.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 283)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 335, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (350.79, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283471, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (358.34, 170.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675294 reads in 248.714 CPU sec, 7.945 real sec
[M::mem_process_seqs] Processed 680664 reads in 181.257 CPU sec, 5.802 real sec
sorting block 132, #reads = 1057665 mem = 536879407 peak = 1069322240
[M::mem_process_seqs] Processed 680164 reads in 197.211 CPU sec, 6.341 real sec
sorting block 133, #reads = 1057146 mem = 536881805 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282888, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (357.84, 165.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679232 sequences (100000024 bp) in 2.703 CPU sec, 2.891 real sec...
[M::mem_process_seqs] Processed 679824 reads in 89.415 CPU sec, 2.939 real sec
sorting block 134, #reads = 1059658 mem = 536877263 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283314, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (360.87, 165.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678970 sequences (100000059 bp) in 2.256 CPU sec, 2.384 real sec...
[M::mem_process_seqs] Processed 679232 reads in 82.013 CPU sec, 2.651 real sec
[M::process] read 680074 sequences (100000112 bp) in 2.127 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283017, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (362.47, 167.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678948 sequences (100000099 bp) in 1.650 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283589, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 192, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (186.67, 61.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (353.02, 163.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 678970 reads in 156.369 CPU sec, 4.904 real sec
sorting block 135, #reads = 1062049 mem = 536880120 peak = 1069322240
[M::mem_process_seqs] Processed 680074 reads in 101.485 CPU sec, 3.180 real sec
[M::process] read 678348 sequences (100000187 bp) in 2.056 CPU sec, 2.099 real sec...
sorting block 136, #reads = 1060873 mem = 536878635 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283226, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (361.96, 165.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678408 sequences (100000252 bp) in 2.097 CPU sec, 2.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282533, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 356, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (368.03, 169.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677316 sequences (100000294 bp) in 1.830 CPU sec, 1.830 real sec...
[M::mem_process_seqs] Processed 678948 reads in 166.897 CPU sec, 5.301 real sec
[M::mem_process_seqs] Processed 678348 reads in 103.868 CPU sec, 3.278 real sec
sorting block 137, #reads = 1058708 mem = 536879934 peak = 1069322240
[M::process] read 677602 sequences (100000104 bp) in 2.147 CPU sec, 2.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282034, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 354, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (367.56, 169.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676764 sequences (100000171 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282221, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 367, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (376.14, 170.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678408 reads in 164.862 CPU sec, 5.241 real sec
[M::mem_process_seqs] Processed 677316 reads in 100.987 CPU sec, 3.221 real sec
sorting block 138, #reads = 1061647 mem = 536881329 peak = 1069322240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282469, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 220, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (178.45, 55.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 366, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (375.17, 169.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676310 sequences (100000197 bp) in 2.203 CPU sec, 2.301 real sec...
[M::process] read 676148 sequences (100000056 bp) in 1.810 CPU sec, 1.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281979, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 373, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (381.17, 171.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677602 reads in 169.384 CPU sec, 5.378 real sec
sorting block 139, #reads = 1059863 mem = 536880049 peak = 1069322240
[M::mem_process_seqs] Processed 676764 reads in 105.975 CPU sec, 3.352 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281777, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 375, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (383.32, 171.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675632 sequences (100000046 bp) in 2.541 CPU sec, 2.635 real sec...
[M::process] read 675694 sequences (100000099 bp) in 1.450 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281358, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 374, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (382.31, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676310 reads in 162.588 CPU sec, 5.158 real sec
sorting block 140, #reads = 1060048 mem = 536881428 peak = 1069322240
[M::mem_process_seqs] Processed 676148 reads in 99.165 CPU sec, 3.143 real sec
sorting block 141, #reads = 1059938 mem = 536879681 peak = 1105764352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281369, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 377, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (384.84, 169.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675506 sequences (100000178 bp) in 2.264 CPU sec, 2.355 real sec...
[M::process] read 675394 sequences (100000281 bp) in 1.750 CPU sec, 1.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281771, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 376, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (384.84, 170.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675632 reads in 159.902 CPU sec, 5.065 real sec
[M::mem_process_seqs] Processed 675694 reads in 97.082 CPU sec, 3.066 real sec
sorting block 142, #reads = 1060238 mem = 536882405 peak = 1105764352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280886, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (388.92, 173.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675212 sequences (100000009 bp) in 2.443 CPU sec, 2.534 real sec...
[M::process] read 674974 sequences (100000143 bp) in 1.380 CPU sec, 1.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281179, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 379, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (388.90, 174.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675506 reads in 150.574 CPU sec, 4.775 real sec
[M::mem_process_seqs] Processed 675394 reads in 92.490 CPU sec, 2.924 real sec
sorting block 143, #reads = 1061472 mem = 536880683 peak = 1105764352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280148, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (389.79, 172.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675114 sequences (100000271 bp) in 2.289 CPU sec, 2.387 real sec...
[M::process] read 674622 sequences (100000279 bp) in 1.820 CPU sec, 1.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280551, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 379, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (388.09, 170.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675212 reads in 159.414 CPU sec, 5.045 real sec
sorting block 144, #reads = 1061294 mem = 536880317 peak = 1105764352
[M::mem_process_seqs] Processed 674974 reads in 98.313 CPU sec, 3.110 real sec
sorting block 145, #reads = 1061321 mem = 536879426 peak = 1105764352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281112, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (389.48, 172.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674616 sequences (100000171 bp) in 2.196 CPU sec, 2.294 real sec...
[M::process] read 674638 sequences (100000107 bp) in 1.400 CPU sec, 1.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280084, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 257, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (242.00, 69.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (393.13, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675114 reads in 153.881 CPU sec, 4.890 real sec
[M::mem_process_seqs] Processed 674622 reads in 95.877 CPU sec, 3.044 real sec
sorting block 146, #reads = 1062448 mem = 536878133 peak = 1105764352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280509, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 417, 1210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3198)
[M::mem_pestat] mean and std.dev: (627.18, 448.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4192)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (392.93, 172.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674478 sequences (100000135 bp) in 2.313 CPU sec, 2.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280616, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (395.68, 175.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674514 sequences (100000213 bp) in 1.780 CPU sec, 1.774 real sec...
[M::mem_process_seqs] Processed 674616 reads in 151.956 CPU sec, 4.812 real sec
[M::mem_process_seqs] Processed 674638 reads in 94.261 CPU sec, 2.970 real sec
sorting block 147, #reads = 1062025 mem = 536878610 peak = 1105764352
[M::process] read 353632 sequences (52344379 bp) in 1.722 CPU sec, 1.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281204, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 389, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (398.50, 177.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280966, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 390, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (399.69, 178.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674478 reads in 149.022 CPU sec, 4.796 real sec
[M::mem_process_seqs] Processed 674514 reads in 91.232 CPU sec, 2.952 real sec
sorting block 148, #reads = 1062361 mem = 536880775 peak = 1105764352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 146979, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (387.43, 173.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 353632 reads in 46.309 CPU sec, 1.542 real sec
sorting block 149, #reads = 1062608 mem = 536879060 peak = 1105764352
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 159116434
overall: 69888143360 mem 23625.147 user 492.840 sys 804.294 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.L002\tPU:L002\tSM:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tLB:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R1.fastq.gz FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 804.329 sec; CPU: 24117.990 sec
sorting block 150, #reads = 291756 mem = 148357149 peak = 1105764352
uniq header count 1
merging 151 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8906512164
output reads: 159836458
bam_mem_sort: 151 calls 99.281 user 1.534 sys 102.763 real
bam_write: 151 calls 75.355 user 4.217 sys 133.175 real
execute: 1 calls 44.281 user 1.294 sys 861.290 real
merge_files: 1 calls 44.257 user 1.273 sys 56.810 real
parse_chunk: 2405 calls 106.517 user 27.995 sys 156.642 real
read_chunk: 6488 calls 54.215 user 43.940 sys 748.060 real
sort_block: 1 calls 36.107 user 43.336 sys 723.091 real
write_chunk: 4081 calls 43.902 user 0.900 sys 55.766 real
overall: 3104960512 mem 2263.917 user 95.522 sys 861.311 real
ls: cannot access '*.cram': No such file or directory