This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cVw-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1630.464 CPU sec, 57.151 real sec
[M::process] read 680910 sequences (100000182 bp) in 0.944 CPU sec, 0.954 real sec...
[M::process] read 680960 sequences (100000277 bp) in 1.207 CPU sec, 1.253 real sec...
[M::process] read 680968 sequences (100000252 bp) in 1.189 CPU sec, 1.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283789, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (329.83, 156.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680796 sequences (100000252 bp) in 1.159 CPU sec, 1.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283926, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 313, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (332.60, 157.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283941, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 312, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (331.17, 156.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680910 reads in 206.100 CPU sec, 6.612 real sec
[M::mem_process_seqs] Processed 680960 reads in 136.687 CPU sec, 4.421 real sec
[M::mem_process_seqs] Processed 680968 reads in 147.501 CPU sec, 4.793 real sec
sorting block 0, #reads = 1085101 mem = 536879274 peak = 586219520
[M::process] read 680384 sequences (100000182 bp) in 1.796 CPU sec, 1.962 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283634, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 312, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (330.70, 155.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680306 sequences (100000015 bp) in 1.249 CPU sec, 1.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283286, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 314, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (332.79, 156.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680796 reads in 132.748 CPU sec, 4.231 real sec
[M::process] read 680058 sequences (100000153 bp) in 1.226 CPU sec, 1.239 real sec...
sorting block 1, #reads = 1085676 mem = 536878975 peak = 892981248
[M::mem_process_seqs] Processed 680384 reads in 82.016 CPU sec, 2.590 real sec
sorting block 2, #reads = 1084900 mem = 536879411 peak = 907370496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283167, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (336.02, 158.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679910 sequences (100000119 bp) in 1.620 CPU sec, 1.740 real sec...
[M::process] read 679488 sequences (100000094 bp) in 1.273 CPU sec, 1.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283223, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 318, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (337.20, 159.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680306 reads in 133.664 CPU sec, 4.272 real sec
[M::mem_process_seqs] Processed 680058 reads in 82.724 CPU sec, 2.648 real sec
sorting block 3, #reads = 1084746 mem = 536881139 peak = 907370496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282974, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 320, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (339.10, 159.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681104 sequences (100000104 bp) in 1.835 CPU sec, 1.935 real sec...
[M::process] read 679208 sequences (100000095 bp) in 1.248 CPU sec, 1.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283502, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (341.96, 160.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679910 reads in 135.581 CPU sec, 4.331 real sec
sorting block 4, #reads = 1084138 mem = 536878983 peak = 907370496
[M::process] read 678676 sequences (100000246 bp) in 1.279 CPU sec, 1.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284452, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 311, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (332.45, 159.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681104 reads in 79.275 CPU sec, 2.678 real sec
[M::mem_process_seqs] Processed 679488 reads in 180.214 CPU sec, 5.918 real sec
sorting block 5, #reads = 1083623 mem = 536879126 peak = 907370496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283218, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (343.51, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678450 sequences (100000131 bp) in 1.834 CPU sec, 1.981 real sec...
[M::process] read 678060 sequences (100000145 bp) in 1.260 CPU sec, 1.253 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282382, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (348.37, 162.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679208 reads in 147.249 CPU sec, 4.706 real sec
[M::mem_process_seqs] Processed 678676 reads in 84.736 CPU sec, 2.697 real sec
sorting block 6, #reads = 1083772 mem = 536878456 peak = 907370496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282397, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (349.85, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677746 sequences (100000094 bp) in 1.780 CPU sec, 1.886 real sec...
[M::process] read 677778 sequences (100000054 bp) in 1.300 CPU sec, 1.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282915, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (352.55, 163.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678450 reads in 135.644 CPU sec, 4.346 real sec
[M::mem_process_seqs] Processed 678060 reads in 82.780 CPU sec, 2.644 real sec
sorting block 7, #reads = 1083591 mem = 536882466 peak = 907370496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282564, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (354.78, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678114 sequences (100000193 bp) in 1.953 CPU sec, 2.075 real sec...
[M::process] read 677512 sequences (100000047 bp) in 1.330 CPU sec, 1.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281712, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (352.72, 162.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677746 reads in 136.657 CPU sec, 4.370 real sec
sorting block 8, #reads = 1083216 mem = 536876976 peak = 907370496
[M::mem_process_seqs] Processed 677778 reads in 84.493 CPU sec, 2.692 real sec
sorting block 9, #reads = 1082605 mem = 536884374 peak = 907370496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282511, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (351.02, 162.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677632 sequences (100000004 bp) in 2.094 CPU sec, 2.255 real sec...
[M::process] read 678586 sequences (100000187 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282199, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (353.48, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678114 reads in 137.272 CPU sec, 4.385 real sec
[M::mem_process_seqs] Processed 677512 reads in 84.647 CPU sec, 2.695 real sec
sorting block 10, #reads = 1082975 mem = 536878235 peak = 907370496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282315, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (351.40, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677168 sequences (100000178 bp) in 2.216 CPU sec, 2.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283067, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (344.43, 161.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676880 sequences (100000248 bp) in 1.370 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 677632 reads in 139.191 CPU sec, 4.443 real sec
[M::mem_process_seqs] Processed 678586 reads in 86.150 CPU sec, 2.739 real sec
sorting block 11, #reads = 1083508 mem = 536878464 peak = 907370496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281986, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.02, 161.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676824 sequences (100000014 bp) in 2.249 CPU sec, 2.430 real sec...
[M::process] read 676858 sequences (100000287 bp) in 1.350 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281899, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (353.96, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677168 reads in 140.407 CPU sec, 4.504 real sec
[M::mem_process_seqs] Processed 676880 reads in 84.056 CPU sec, 2.693 real sec
sorting block 12, #reads = 1083629 mem = 536877756 peak = 907370496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281504, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (355.51, 162.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676608 sequences (100000140 bp) in 2.249 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281497, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (356.39, 162.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676616 sequences (100000214 bp) in 1.340 CPU sec, 1.348 real sec...
[M::mem_process_seqs] Processed 676824 reads in 134.733 CPU sec, 4.291 real sec
sorting block 13, #reads = 1083873 mem = 536879504 peak = 907370496
[M::mem_process_seqs] Processed 676858 reads in 84.188 CPU sec, 2.671 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282548, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 342, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (356.88, 162.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1083177 mem = 536878857 peak = 948420608
[M::process] read 676508 sequences (100000075 bp) in 2.142 CPU sec, 2.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281669, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 339, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (354.49, 160.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676670 sequences (100000266 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 676608 reads in 134.011 CPU sec, 4.272 real sec
[M::mem_process_seqs] Processed 676616 reads in 83.697 CPU sec, 2.655 real sec
sorting block 15, #reads = 1082872 mem = 536878657 peak = 948420608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282264, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 339, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (353.98, 160.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676724 sequences (100000180 bp) in 2.191 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281714, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (353.13, 160.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676928 sequences (100000128 bp) in 1.370 CPU sec, 1.362 real sec...
[M::mem_process_seqs] Processed 676508 reads in 134.210 CPU sec, 4.271 real sec
[M::mem_process_seqs] Processed 676670 reads in 84.664 CPU sec, 2.684 real sec
sorting block 16, #reads = 1081916 mem = 536877854 peak = 948420608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281565, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (352.86, 160.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676728 sequences (100000168 bp) in 2.198 CPU sec, 2.324 real sec...
[M::process] read 677066 sequences (100000216 bp) in 1.310 CPU sec, 1.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282024, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (352.34, 160.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676724 reads in 134.828 CPU sec, 4.291 real sec
[M::mem_process_seqs] Processed 676928 reads in 83.490 CPU sec, 2.652 real sec
sorting block 17, #reads = 1081283 mem = 536879995 peak = 948420608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281747, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (350.49, 159.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676728 sequences (100000188 bp) in 2.204 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281750, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.85, 158.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676492 sequences (100000236 bp) in 1.350 CPU sec, 1.350 real sec...
[M::mem_process_seqs] Processed 676728 reads in 133.233 CPU sec, 4.239 real sec
sorting block 18, #reads = 1082078 mem = 536880335 peak = 948420608
[M::mem_process_seqs] Processed 677066 reads in 83.017 CPU sec, 2.634 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282040, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.09, 158.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1081732 mem = 536878753 peak = 1008013312
[M::process] read 676420 sequences (100000060 bp) in 2.186 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282241, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (354.29, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676360 sequences (100000143 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_process_seqs] Processed 676728 reads in 132.361 CPU sec, 4.215 real sec
[M::mem_process_seqs] Processed 676492 reads in 81.981 CPU sec, 2.603 real sec
sorting block 20, #reads = 1083068 mem = 536878511 peak = 1008013312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281606, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (354.65, 161.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676284 sequences (100000227 bp) in 2.207 CPU sec, 2.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281585, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 339, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (354.78, 161.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675910 sequences (100000095 bp) in 1.320 CPU sec, 1.317 real sec...
[M::mem_process_seqs] Processed 676420 reads in 131.243 CPU sec, 4.180 real sec
[M::mem_process_seqs] Processed 676360 reads in 82.198 CPU sec, 2.604 real sec
sorting block 21, #reads = 1083577 mem = 536877886 peak = 1008013312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281502, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (355.46, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675900 sequences (100000184 bp) in 2.219 CPU sec, 2.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282441, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (358.17, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680360 sequences (100000138 bp) in 1.310 CPU sec, 1.320 real sec...
[M::mem_process_seqs] Processed 676284 reads in 130.043 CPU sec, 4.137 real sec
[M::mem_process_seqs] Processed 675910 reads in 80.841 CPU sec, 2.564 real sec
sorting block 22, #reads = 1083867 mem = 536877514 peak = 1008013312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281410, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (359.23, 163.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680940 sequences (100000045 bp) in 2.188 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283474, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 315, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (335.34, 158.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680316 sequences (100000292 bp) in 1.320 CPU sec, 1.315 real sec...
[M::mem_process_seqs] Processed 675900 reads in 132.885 CPU sec, 4.229 real sec
sorting block 23, #reads = 1082610 mem = 536879740 peak = 1008013312
[M::mem_process_seqs] Processed 680360 reads in 84.280 CPU sec, 2.674 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283429, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (334.21, 159.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1081923 mem = 536878762 peak = 1008013312
[M::process] read 680250 sequences (100000023 bp) in 2.234 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283528, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (335.40, 158.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680406 sequences (100000046 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_process_seqs] Processed 680940 reads in 131.877 CPU sec, 4.196 real sec
[M::mem_process_seqs] Processed 680316 reads in 81.893 CPU sec, 2.595 real sec
sorting block 25, #reads = 1086485 mem = 536885424 peak = 1008013312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283375, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (338.35, 160.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680194 sequences (100000214 bp) in 2.179 CPU sec, 2.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282866, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 319, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (337.63, 159.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680014 sequences (100000207 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 680250 reads in 132.166 CPU sec, 4.208 real sec
[M::mem_process_seqs] Processed 680406 reads in 82.766 CPU sec, 2.624 real sec
sorting block 26, #reads = 1087085 mem = 536878146 peak = 1008013312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282909, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (337.43, 159.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679666 sequences (100000000 bp) in 2.242 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282739, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (340.09, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679488 sequences (100000293 bp) in 1.320 CPU sec, 1.320 real sec...
[M::mem_process_seqs] Processed 680194 reads in 133.001 CPU sec, 4.234 real sec
[M::mem_process_seqs] Processed 680014 reads in 83.446 CPU sec, 2.643 real sec
sorting block 27, #reads = 1086048 mem = 536879337 peak = 1008013312
[M::process] read 679020 sequences (100000183 bp) in 2.345 CPU sec, 2.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283107, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (343.34, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678998 sequences (100000041 bp) in 1.350 CPU sec, 1.346 real sec...
[M::mem_process_seqs] Processed 679488 reads in 77.940 CPU sec, 2.469 real sec
sorting block 28, #reads = 1084706 mem = 536879834 peak = 1008013312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282909, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (341.13, 160.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282885, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (345.14, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679666 reads in 203.408 CPU sec, 6.476 real sec
[M::process] read 678748 sequences (100000240 bp) in 1.505 CPU sec, 1.534 real sec...
[M::mem_process_seqs] Processed 679020 reads in 94.199 CPU sec, 3.013 real sec
sorting block 29, #reads = 1085181 mem = 536884355 peak = 1017790464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283305, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (348.77, 163.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678108 sequences (100000202 bp) in 2.298 CPU sec, 2.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283187, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (351.54, 164.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678998 reads in 132.320 CPU sec, 4.224 real sec
[M::process] read 677998 sequences (100000254 bp) in 1.366 CPU sec, 1.370 real sec...
sorting block 30, #reads = 1084727 mem = 536881409 peak = 1017790464
[M::mem_process_seqs] Processed 678748 reads in 82.369 CPU sec, 2.606 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282447, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (352.99, 163.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1084765 mem = 536878446 peak = 1017790464
[M::process] read 677888 sequences (100000150 bp) in 2.137 CPU sec, 2.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282222, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (355.37, 164.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677400 sequences (100000158 bp) in 1.360 CPU sec, 1.364 real sec...
[M::mem_process_seqs] Processed 678108 reads in 133.000 CPU sec, 4.232 real sec
[M::mem_process_seqs] Processed 677998 reads in 83.297 CPU sec, 2.641 real sec
sorting block 32, #reads = 1083485 mem = 536877039 peak = 1017790464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282468, 1, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (356.59, 165.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1668, 1668, 1668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1668, 1668)
[M::mem_pestat] mean and std.dev: (1668.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1668, 1668)
[M::mem_pestat] skip orientation RR
[M::process] read 677482 sequences (100000018 bp) in 2.047 CPU sec, 2.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282332, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (357.23, 164.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677352 sequences (100000225 bp) in 1.340 CPU sec, 1.343 real sec...
[M::mem_process_seqs] Processed 677888 reads in 133.814 CPU sec, 4.264 real sec
[M::mem_process_seqs] Processed 677400 reads in 83.832 CPU sec, 2.657 real sec
sorting block 33, #reads = 1083839 mem = 536879124 peak = 1017790464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282079, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (356.13, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677550 sequences (100000133 bp) in 2.189 CPU sec, 2.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282220, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (355.84, 163.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677364 sequences (100000300 bp) in 1.320 CPU sec, 1.317 real sec...
[M::mem_process_seqs] Processed 677482 reads in 131.186 CPU sec, 4.168 real sec
[M::mem_process_seqs] Processed 677352 reads in 81.498 CPU sec, 2.581 real sec
sorting block 34, #reads = 1083125 mem = 536879888 peak = 1017790464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282852, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 343, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (357.19, 163.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677242 sequences (100000242 bp) in 2.137 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282429, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.19, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676942 sequences (100000150 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_process_seqs] Processed 677550 reads in 129.210 CPU sec, 4.103 real sec
sorting block 35, #reads = 1083292 mem = 536878411 peak = 1017790464
[M::mem_process_seqs] Processed 677364 reads in 80.355 CPU sec, 2.546 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282200, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (355.28, 162.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1083941 mem = 536882791 peak = 1068261376
[M::process] read 676494 sequences (100000108 bp) in 2.254 CPU sec, 2.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281858, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 341, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (355.41, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676752 sequences (100000291 bp) in 1.330 CPU sec, 1.335 real sec...
[M::mem_process_seqs] Processed 677242 reads in 130.244 CPU sec, 4.135 real sec
[M::mem_process_seqs] Processed 676942 reads in 82.155 CPU sec, 2.598 real sec
sorting block 37, #reads = 1082897 mem = 536880182 peak = 1068261376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281585, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (357.45, 162.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676800 sequences (100000010 bp) in 2.196 CPU sec, 2.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282086, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 340, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (355.04, 160.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676696 sequences (100000119 bp) in 1.360 CPU sec, 1.350 real sec...
[M::mem_process_seqs] Processed 676494 reads in 130.565 CPU sec, 4.148 real sec
[M::mem_process_seqs] Processed 676752 reads in 82.006 CPU sec, 2.594 real sec
sorting block 38, #reads = 1082567 mem = 536881321 peak = 1068261376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282252, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 341, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (355.09, 160.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676420 sequences (100000238 bp) in 2.127 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281759, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 344, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (358.96, 163.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676300 sequences (100000055 bp) in 1.320 CPU sec, 1.321 real sec...
[M::mem_process_seqs] Processed 676800 reads in 130.058 CPU sec, 4.134 real sec
[M::mem_process_seqs] Processed 676696 reads in 82.082 CPU sec, 2.595 real sec
sorting block 39, #reads = 1082563 mem = 536879141 peak = 1068261376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281698, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 340, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (355.42, 160.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676368 sequences (100000246 bp) in 2.302 CPU sec, 2.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281669, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 341, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (355.20, 159.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676748 sequences (100000292 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 676420 reads in 131.594 CPU sec, 4.178 real sec
sorting block 40, #reads = 1082813 mem = 536877586 peak = 1068261376
[M::mem_process_seqs] Processed 676300 reads in 82.179 CPU sec, 2.603 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281995, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (352.65, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1082020 mem = 536879758 peak = 1068261376
[M::process] read 676850 sequences (100000226 bp) in 2.173 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281982, 4, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (352.42, 159.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1382, 1382, 1382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1382, 1382)
[M::mem_pestat] mean and std.dev: (1382.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1382, 1382)
[M::mem_pestat] skip orientation RR
[M::process] read 676704 sequences (100000063 bp) in 1.310 CPU sec, 1.312 real sec...
[M::mem_process_seqs] Processed 676368 reads in 132.207 CPU sec, 4.203 real sec
[M::mem_process_seqs] Processed 676748 reads in 82.749 CPU sec, 2.620 real sec
sorting block 42, #reads = 1081776 mem = 536878874 peak = 1068261376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281460, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (351.73, 159.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676712 sequences (100000006 bp) in 2.225 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282423, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (353.25, 160.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676962 sequences (100000234 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_process_seqs] Processed 676850 reads in 131.776 CPU sec, 4.185 real sec
[M::mem_process_seqs] Processed 676704 reads in 81.520 CPU sec, 2.585 real sec
sorting block 43, #reads = 1081695 mem = 536878432 peak = 1068261376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282375, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 336, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (352.14, 160.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676770 sequences (100000136 bp) in 2.224 CPU sec, 2.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281922, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (350.52, 159.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676654 sequences (100000274 bp) in 1.300 CPU sec, 1.298 real sec...
[M::mem_process_seqs] Processed 676712 reads in 129.195 CPU sec, 4.102 real sec
[M::mem_process_seqs] Processed 676962 reads in 81.303 CPU sec, 2.573 real sec
sorting block 44, #reads = 1082026 mem = 536880042 peak = 1068261376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282360, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (351.54, 159.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676806 sequences (100000040 bp) in 2.047 CPU sec, 2.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282184, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.97, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676586 sequences (100000020 bp) in 1.330 CPU sec, 1.332 real sec...
[M::mem_process_seqs] Processed 676770 reads in 128.710 CPU sec, 4.090 real sec
[M::mem_process_seqs] Processed 676654 reads in 81.164 CPU sec, 2.567 real sec
sorting block 45, #reads = 1082051 mem = 536878191 peak = 1068261376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281995, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (351.30, 160.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1082775 mem = 536878569 peak = 1089208320
[M::process] read 676490 sequences (100000231 bp) in 2.053 CPU sec, 2.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282843, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 340, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (356.53, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678220 sequences (100000204 bp) in 1.310 CPU sec, 1.311 real sec...
[M::mem_process_seqs] Processed 676806 reads in 129.499 CPU sec, 4.118 real sec
[M::mem_process_seqs] Processed 676586 reads in 81.574 CPU sec, 2.583 real sec
sorting block 47, #reads = 1082353 mem = 536878847 peak = 1089208320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282191, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 342, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (358.52, 164.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681354 sequences (100000126 bp) in 2.320 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282953, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (349.06, 162.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676490 reads in 129.435 CPU sec, 4.108 real sec
[M::process] read 681188 sequences (100000294 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_process_seqs] Processed 678220 reads in 81.869 CPU sec, 2.589 real sec
sorting block 48, #reads = 1081288 mem = 536880870 peak = 1089208320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283817, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (332.54, 158.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680658 sequences (100000196 bp) in 2.213 CPU sec, 2.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284049, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (333.42, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680558 sequences (100000075 bp) in 1.320 CPU sec, 1.322 real sec...
[M::mem_process_seqs] Processed 681354 reads in 130.667 CPU sec, 4.148 real sec
[M::mem_process_seqs] Processed 681188 reads in 81.765 CPU sec, 2.588 real sec
sorting block 49, #reads = 1084024 mem = 536879760 peak = 1089208320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283853, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 315, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (334.82, 158.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680086 sequences (100000292 bp) in 2.295 CPU sec, 2.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283576, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (335.73, 158.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679936 sequences (100000234 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_process_seqs] Processed 680658 reads in 129.374 CPU sec, 4.106 real sec
sorting block 50, #reads = 1084363 mem = 536877383 peak = 1089208320
[M::mem_process_seqs] Processed 680558 reads in 81.013 CPU sec, 2.563 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283563, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 51, #reads = 1085529 mem = 536881277 peak = 1100996608
[M::mem_pestat] (25, 50, 75) percentile: (211, 320, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (337.77, 158.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680046 sequences (100000087 bp) in 2.247 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283377, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 321, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (338.70, 158.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679394 sequences (100000079 bp) in 1.330 CPU sec, 1.333 real sec...
[M::mem_process_seqs] Processed 680086 reads in 130.385 CPU sec, 4.140 real sec
[M::mem_process_seqs] Processed 679936 reads in 82.196 CPU sec, 2.599 real sec
sorting block 52, #reads = 1084302 mem = 536881680 peak = 1100996608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283245, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (339.59, 159.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679116 sequences (100000054 bp) in 2.254 CPU sec, 2.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282944, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (342.13, 159.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678980 sequences (100000256 bp) in 1.360 CPU sec, 1.359 real sec...
[M::mem_process_seqs] Processed 680046 reads in 129.962 CPU sec, 4.126 real sec
[M::mem_process_seqs] Processed 679394 reads in 81.678 CPU sec, 2.590 real sec
sorting block 53, #reads = 1083967 mem = 536879778 peak = 1100996608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283374, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (343.97, 159.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678628 sequences (100000174 bp) in 2.233 CPU sec, 2.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283291, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (346.27, 161.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678452 sequences (100000061 bp) in 1.310 CPU sec, 1.318 real sec...
[M::mem_process_seqs] Processed 679116 reads in 129.290 CPU sec, 4.108 real sec
[M::mem_process_seqs] Processed 678980 reads in 80.930 CPU sec, 2.561 real sec
sorting block 54, #reads = 1083492 mem = 536878456 peak = 1100996608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282956, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (348.87, 162.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678202 sequences (100000120 bp) in 2.034 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283049, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (352.54, 165.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677804 sequences (100000144 bp) in 1.350 CPU sec, 1.350 real sec...
[M::mem_process_seqs] Processed 678628 reads in 128.658 CPU sec, 4.089 real sec
sorting block 55, #reads = 1083895 mem = 536881216 peak = 1100996608
[M::mem_process_seqs] Processed 678452 reads in 80.509 CPU sec, 2.545 real sec
sorting block 56, #reads = 1083604 mem = 536879763 peak = 1146486784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282453, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (353.77, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677714 sequences (100000217 bp) in 2.183 CPU sec, 2.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282470, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (356.30, 165.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677530 sequences (100000013 bp) in 1.330 CPU sec, 1.329 real sec...
[M::mem_process_seqs] Processed 678202 reads in 130.373 CPU sec, 4.144 real sec
[M::mem_process_seqs] Processed 677804 reads in 82.761 CPU sec, 2.621 real sec
sorting block 57, #reads = 1083885 mem = 536886093 peak = 1146486784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282654, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (356.95, 165.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677682 sequences (100000055 bp) in 2.348 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282875, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (356.34, 164.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677714 reads in 130.094 CPU sec, 4.131 real sec
[M::process] read 677584 sequences (100000094 bp) in 1.360 CPU sec, 1.358 real sec...
[M::mem_process_seqs] Processed 677530 reads in 81.268 CPU sec, 2.566 real sec
sorting block 58, #reads = 1082901 mem = 536880874 peak = 1146486784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282948, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (355.54, 164.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677318 sequences (100000292 bp) in 2.102 CPU sec, 2.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282674, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 340, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (356.36, 165.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677372 sequences (100000145 bp) in 1.320 CPU sec, 1.320 real sec...
[M::mem_process_seqs] Processed 677682 reads in 130.178 CPU sec, 4.139 real sec
[M::mem_process_seqs] Processed 677584 reads in 82.314 CPU sec, 2.606 real sec
sorting block 59, #reads = 1082914 mem = 536879739 peak = 1146486784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282828, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (356.46, 164.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677498 sequences (100000067 bp) in 2.242 CPU sec, 2.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282175, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 340, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (356.42, 164.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677374 sequences (100000015 bp) in 1.350 CPU sec, 1.347 real sec...
[M::mem_process_seqs] Processed 677318 reads in 130.622 CPU sec, 4.150 real sec
sorting block 60, #reads = 1082254 mem = 536879094 peak = 1146486784
[M::mem_process_seqs] Processed 677372 reads in 82.337 CPU sec, 2.609 real sec
sorting block 61, #reads = 1082709 mem = 536876346 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282169, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (354.56, 164.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677342 sequences (100000122 bp) in 2.244 CPU sec, 2.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282472, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.21, 163.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677454 sequences (100000275 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 677498 reads in 131.539 CPU sec, 4.181 real sec
[M::mem_process_seqs] Processed 677374 reads in 82.955 CPU sec, 2.623 real sec
sorting block 62, #reads = 1082947 mem = 536878536 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282372, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.64, 163.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677544 sequences (100000258 bp) in 2.341 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282281, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (354.80, 164.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677576 sequences (100000247 bp) in 1.350 CPU sec, 1.346 real sec...
[M::mem_process_seqs] Processed 677342 reads in 132.613 CPU sec, 4.211 real sec
[M::mem_process_seqs] Processed 677454 reads in 82.768 CPU sec, 2.620 real sec
sorting block 63, #reads = 1082200 mem = 536878728 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282435, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (352.85, 163.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677536 sequences (100000067 bp) in 2.210 CPU sec, 2.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281876, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (349.52, 162.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677478 sequences (100000113 bp) in 1.330 CPU sec, 1.328 real sec...
[M::mem_process_seqs] Processed 677544 reads in 132.033 CPU sec, 4.197 real sec
sorting block 64, #reads = 1082335 mem = 536879185 peak = 1152811008
[M::mem_process_seqs] Processed 677576 reads in 82.396 CPU sec, 2.610 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282157, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (348.02, 161.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 65, #reads = 1082626 mem = 536879397 peak = 1152811008
[M::process] read 677320 sequences (100000290 bp) in 2.343 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282740, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (350.92, 162.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677484 sequences (100000052 bp) in 1.350 CPU sec, 1.354 real sec...
[M::mem_process_seqs] Processed 677536 reads in 131.748 CPU sec, 4.183 real sec
[M::mem_process_seqs] Processed 677478 reads in 82.786 CPU sec, 2.617 real sec
sorting block 66, #reads = 1082693 mem = 536877830 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282295, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (351.05, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677192 sequences (100000120 bp) in 2.348 CPU sec, 2.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282484, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (349.50, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677080 sequences (100000177 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 677320 reads in 132.277 CPU sec, 4.200 real sec
[M::mem_process_seqs] Processed 677484 reads in 83.154 CPU sec, 2.630 real sec
sorting block 67, #reads = 1081822 mem = 536879269 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282039, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (349.03, 159.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676974 sequences (100000212 bp) in 2.206 CPU sec, 2.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282472, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (351.11, 160.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676974 sequences (100000199 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 677192 reads in 132.826 CPU sec, 4.221 real sec
[M::mem_process_seqs] Processed 677080 reads in 82.168 CPU sec, 2.606 real sec
sorting block 68, #reads = 1082493 mem = 536878530 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282365, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.34, 161.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676632 sequences (100000105 bp) in 2.066 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282201, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (353.94, 162.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676974 reads in 130.030 CPU sec, 4.146 real sec
[M::process] read 676714 sequences (100000095 bp) in 1.354 CPU sec, 1.362 real sec...
[M::mem_process_seqs] Processed 676974 reads in 81.274 CPU sec, 2.569 real sec
sorting block 69, #reads = 1082499 mem = 536879133 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282310, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (355.03, 163.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 70, #reads = 1081647 mem = 536880252 peak = 1152811008
[M::process] read 676862 sequences (100000158 bp) in 2.175 CPU sec, 2.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282713, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (354.83, 162.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676632 reads in 129.446 CPU sec, 4.125 real sec
[M::process] read 676822 sequences (100000217 bp) in 1.427 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 676714 reads in 81.631 CPU sec, 2.579 real sec
sorting block 71, #reads = 1082245 mem = 536880536 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282340, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (352.18, 162.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677788 sequences (100000203 bp) in 2.371 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282286, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (353.88, 162.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676862 reads in 128.686 CPU sec, 4.091 real sec
[M::process] read 681292 sequences (100000063 bp) in 1.406 CPU sec, 1.422 real sec...
[M::mem_process_seqs] Processed 676822 reads in 81.222 CPU sec, 2.567 real sec
sorting block 72, #reads = 1082391 mem = 536885358 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282540, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (350.45, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680722 sequences (100000077 bp) in 2.275 CPU sec, 2.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284026, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 313, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (334.93, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680238 sequences (100000070 bp) in 1.330 CPU sec, 1.325 real sec...
[M::mem_process_seqs] Processed 677788 reads in 133.001 CPU sec, 4.229 real sec
[M::mem_process_seqs] Processed 681292 reads in 81.494 CPU sec, 2.584 real sec
sorting block 73, #reads = 1082047 mem = 536879474 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283555, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (338.90, 163.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680078 sequences (100000012 bp) in 2.230 CPU sec, 2.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283444, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (341.33, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679736 sequences (100000035 bp) in 1.300 CPU sec, 1.297 real sec...
[M::mem_process_seqs] Processed 680722 reads in 128.130 CPU sec, 4.072 real sec
sorting block 74, #reads = 1085633 mem = 536879067 peak = 1152811008
[M::mem_process_seqs] Processed 680238 reads in 79.923 CPU sec, 2.530 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283349, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (342.32, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 75, #reads = 1086599 mem = 536879065 peak = 1152811008
[M::process] read 679650 sequences (100000271 bp) in 1.946 CPU sec, 2.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282732, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (343.40, 163.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679522 sequences (100000053 bp) in 1.330 CPU sec, 1.326 real sec...
[M::mem_process_seqs] Processed 680078 reads in 128.178 CPU sec, 4.085 real sec
[M::mem_process_seqs] Processed 679736 reads in 80.412 CPU sec, 2.549 real sec
sorting block 76, #reads = 1085552 mem = 536880730 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282982, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (343.76, 163.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678960 sequences (100000123 bp) in 2.318 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282722, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (345.40, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679650 reads in 127.596 CPU sec, 4.052 real sec
[M::process] read 678804 sequences (100000104 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 679522 reads in 80.712 CPU sec, 2.554 real sec
sorting block 77, #reads = 1085335 mem = 536880773 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282868, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (346.60, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678498 sequences (100000007 bp) in 2.244 CPU sec, 2.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282820, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (347.95, 164.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678452 sequences (100000179 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_process_seqs] Processed 678960 reads in 128.419 CPU sec, 4.078 real sec
[M::mem_process_seqs] Processed 678804 reads in 81.133 CPU sec, 2.564 real sec
sorting block 78, #reads = 1084882 mem = 536879738 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283043, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (349.28, 163.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678392 sequences (100000219 bp) in 2.276 CPU sec, 2.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282963, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.84, 164.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678498 reads in 127.654 CPU sec, 4.060 real sec
[M::process] read 678160 sequences (100000274 bp) in 1.355 CPU sec, 1.358 real sec...
sorting block 79, #reads = 1083692 mem = 536878433 peak = 1152811008
[M::mem_process_seqs] Processed 678452 reads in 80.796 CPU sec, 2.551 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282871, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (352.11, 165.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1084199 mem = 536878237 peak = 1152811008
[M::process] read 678028 sequences (100000292 bp) in 2.235 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283373, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (353.41, 165.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678392 reads in 127.752 CPU sec, 4.061 real sec
[M::process] read 677684 sequences (100000269 bp) in 1.350 CPU sec, 1.350 real sec...
[M::mem_process_seqs] Processed 678160 reads in 79.861 CPU sec, 2.525 real sec
sorting block 81, #reads = 1084644 mem = 536882073 peak = 1152811008
[M::process] read 677616 sequences (100000194 bp) in 2.115 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282295, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 339, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (356.94, 167.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677866 sequences (100000107 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 677684 reads in 75.083 CPU sec, 2.372 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283028, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (355.72, 166.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282559, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 340, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (357.79, 167.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678028 reads in 196.682 CPU sec, 6.250 real sec
sorting block 82, #reads = 1083034 mem = 536879600 peak = 1152811008
[M::process] read 677730 sequences (100000163 bp) in 1.607 CPU sec, 1.622 real sec...
[M::mem_process_seqs] Processed 677616 reads in 92.114 CPU sec, 2.928 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282545, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (358.83, 168.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 83, #reads = 1085001 mem = 536881385 peak = 1152811008
[M::process] read 677418 sequences (100000017 bp) in 2.144 CPU sec, 2.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282563, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (358.51, 168.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677866 reads in 128.724 CPU sec, 4.101 real sec
[M::process] read 677630 sequences (100000227 bp) in 1.462 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 677730 reads in 82.007 CPU sec, 2.585 real sec
sorting block 84, #reads = 1084363 mem = 536879171 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282410, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (358.79, 168.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677800 sequences (100000132 bp) in 1.869 CPU sec, 1.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282740, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (355.75, 166.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677972 sequences (100000083 bp) in 1.340 CPU sec, 1.348 real sec...
[M::mem_process_seqs] Processed 677418 reads in 128.902 CPU sec, 4.104 real sec
[M::mem_process_seqs] Processed 677630 reads in 81.214 CPU sec, 2.570 real sec
sorting block 85, #reads = 1083610 mem = 536880552 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282990, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (354.85, 167.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678082 sequences (100000081 bp) in 2.285 CPU sec, 2.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282785, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (353.21, 166.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677800 reads in 128.575 CPU sec, 4.086 real sec
[M::process] read 678276 sequences (100000199 bp) in 1.447 CPU sec, 1.455 real sec...
[M::mem_process_seqs] Processed 677972 reads in 81.007 CPU sec, 2.555 real sec
sorting block 86, #reads = 1084211 mem = 536884960 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282757, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (351.56, 165.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678176 sequences (100000058 bp) in 1.962 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282896, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (350.95, 165.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678082 reads in 128.949 CPU sec, 4.104 real sec
[M::process] read 678162 sequences (100000110 bp) in 1.380 CPU sec, 1.380 real sec...
sorting block 87, #reads = 1083753 mem = 536881454 peak = 1152811008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282679, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (349.35, 165.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678196 sequences (100000185 bp) in 1.574 CPU sec, 1.584 real sec...
[M::mem_process_seqs] Processed 678176 reads in 75.067 CPU sec, 2.541 real sec
sorting block 88, #reads = 1082826 mem = 536877147 peak = 1183358976
[M::mem_process_seqs] Processed 678276 reads in 178.066 CPU sec, 5.813 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282415, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (348.94, 163.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678304 sequences (100000169 bp) in 1.870 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282513, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (348.37, 163.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678162 reads in 140.689 CPU sec, 4.461 real sec
[M::process] read 678050 sequences (100000004 bp) in 1.454 CPU sec, 1.471 real sec...
sorting block 89, #reads = 1083696 mem = 536878519 peak = 1183358976
[M::mem_process_seqs] Processed 678196 reads in 81.563 CPU sec, 2.580 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283673, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (349.38, 164.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 90, #reads = 1083817 mem = 536881313 peak = 1183358976
[M::process] read 677926 sequences (100000275 bp) in 2.199 CPU sec, 2.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283215, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (348.79, 164.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677920 sequences (100000095 bp) in 1.360 CPU sec, 1.360 real sec...
[M::mem_process_seqs] Processed 678304 reads in 129.029 CPU sec, 4.095 real sec
[M::mem_process_seqs] Processed 678050 reads in 80.422 CPU sec, 2.543 real sec
sorting block 91, #reads = 1083998 mem = 536883195 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282565, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (345.39, 161.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677472 sequences (100000274 bp) in 2.030 CPU sec, 2.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283073, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (347.31, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677926 reads in 128.978 CPU sec, 4.107 real sec
[M::process] read 677742 sequences (100000292 bp) in 1.458 CPU sec, 1.470 real sec...
[M::mem_process_seqs] Processed 677920 reads in 81.719 CPU sec, 2.582 real sec
sorting block 92, #reads = 1083136 mem = 536879575 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282792, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (349.68, 162.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677468 sequences (100000241 bp) in 2.132 CPU sec, 2.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282776, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (349.46, 162.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677442 sequences (100000027 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_process_seqs] Processed 677472 reads in 127.763 CPU sec, 4.058 real sec
[M::mem_process_seqs] Processed 677742 reads in 79.970 CPU sec, 2.528 real sec
sorting block 93, #reads = 1082925 mem = 536881111 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282590, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (350.41, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677482 sequences (100000048 bp) in 2.060 CPU sec, 2.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282083, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (352.54, 163.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677468 reads in 128.619 CPU sec, 4.097 real sec
sorting block 94, #reads = 1083996 mem = 536883440 peak = 1183358976
[M::process] read 677418 sequences (100000184 bp) in 1.509 CPU sec, 1.529 real sec...
[M::mem_process_seqs] Processed 677442 reads in 81.494 CPU sec, 2.573 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282481, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (352.40, 164.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 95, #reads = 1082648 mem = 536879540 peak = 1183358976
[M::process] read 677324 sequences (100000135 bp) in 2.133 CPU sec, 2.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282795, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (352.87, 164.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677482 reads in 127.384 CPU sec, 4.047 real sec
[M::process] read 677230 sequences (100000184 bp) in 1.400 CPU sec, 1.399 real sec...
[M::mem_process_seqs] Processed 677418 reads in 80.347 CPU sec, 2.537 real sec
sorting block 96, #reads = 1082422 mem = 536881971 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282451, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (351.67, 163.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680302 sequences (100000067 bp) in 2.061 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282856, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.86, 164.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677324 reads in 126.958 CPU sec, 4.039 real sec
[M::process] read 682812 sequences (100000069 bp) in 1.548 CPU sec, 1.571 real sec...
[M::mem_process_seqs] Processed 677230 reads in 80.896 CPU sec, 2.553 real sec
sorting block 97, #reads = 1082927 mem = 536883079 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283910, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 316, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (339.35, 164.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683592 sequences (100000297 bp) in 2.165 CPU sec, 2.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285204, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 304, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (331.91, 166.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683012 sequences (100000000 bp) in 1.420 CPU sec, 1.424 real sec...
[M::mem_process_seqs] Processed 680302 reads in 138.493 CPU sec, 4.401 real sec
[M::mem_process_seqs] Processed 682812 reads in 87.040 CPU sec, 2.751 real sec
sorting block 98, #reads = 1082550 mem = 536879873 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285319, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (325.05, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682046 sequences (100000155 bp) in 2.027 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285014, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 302, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (328.99, 163.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681378 sequences (100000278 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 683592 reads in 139.550 CPU sec, 4.448 real sec
sorting block 99, #reads = 1082391 mem = 536879589 peak = 1183358976
[M::mem_process_seqs] Processed 683012 reads in 87.362 CPU sec, 2.761 real sec
sorting block 100, #reads = 1082822 mem = 536879509 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284387, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (334.11, 164.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681482 sequences (100000212 bp) in 2.314 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283895, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 311, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (336.41, 164.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681120 sequences (100000223 bp) in 1.390 CPU sec, 1.386 real sec...
[M::mem_process_seqs] Processed 682046 reads in 137.101 CPU sec, 4.354 real sec
[M::mem_process_seqs] Processed 681378 reads in 85.752 CPU sec, 2.713 real sec
sorting block 101, #reads = 1083819 mem = 536879361 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283751, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 311, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (336.36, 164.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682820 sequences (100000238 bp) in 2.029 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283359, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 314, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (338.64, 165.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680494 sequences (100000000 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 681482 reads in 137.195 CPU sec, 4.367 real sec
[M::mem_process_seqs] Processed 681120 reads in 85.384 CPU sec, 2.704 real sec
sorting block 102, #reads = 1083012 mem = 536881849 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284812, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 296, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (324.74, 161.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680284 sequences (100000273 bp) in 2.215 CPU sec, 2.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283959, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 316, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (339.96, 164.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680220 sequences (100000014 bp) in 1.390 CPU sec, 1.387 real sec...
[M::mem_process_seqs] Processed 682820 reads in 136.357 CPU sec, 4.331 real sec
[M::mem_process_seqs] Processed 680494 reads in 84.821 CPU sec, 2.682 real sec
sorting block 103, #reads = 1083756 mem = 536877446 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283339, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 321, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (343.26, 165.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 104, #reads = 1083983 mem = 536880178 peak = 1183358976
[M::process] read 679614 sequences (100000287 bp) in 1.947 CPU sec, 2.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283608, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 322, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (343.25, 165.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679332 sequences (100000169 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_process_seqs] Processed 680284 reads in 135.597 CPU sec, 4.316 real sec
[M::mem_process_seqs] Processed 680220 reads in 84.666 CPU sec, 2.678 real sec
sorting block 105, #reads = 1084101 mem = 536880857 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283070, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 325, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (345.24, 164.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679864 sequences (100000274 bp) in 2.244 CPU sec, 2.387 real sec...
[M::process] read 679560 sequences (100000296 bp) in 1.380 CPU sec, 1.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283094, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 329, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (349.88, 166.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679614 reads in 140.194 CPU sec, 4.453 real sec
[M::mem_process_seqs] Processed 679332 reads in 88.606 CPU sec, 2.809 real sec
sorting block 106, #reads = 1081417 mem = 536882058 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283719, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 327, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (346.91, 165.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680718 sequences (100000223 bp) in 1.968 CPU sec, 2.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282756, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 327, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (346.82, 164.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679932 sequences (100000193 bp) in 1.380 CPU sec, 1.385 real sec...
[M::mem_process_seqs] Processed 679864 reads in 137.099 CPU sec, 4.364 real sec
[M::mem_process_seqs] Processed 679560 reads in 84.802 CPU sec, 2.688 real sec
sorting block 107, #reads = 1081501 mem = 536880003 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283467, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 313, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (336.92, 162.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678472 sequences (100000241 bp) in 2.204 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283400, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (337.83, 161.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678726 sequences (100000171 bp) in 1.390 CPU sec, 1.385 real sec...
[M::mem_process_seqs] Processed 680718 reads in 139.006 CPU sec, 4.417 real sec
sorting block 108, #reads = 1081820 mem = 536877855 peak = 1183358976
[M::mem_process_seqs] Processed 679932 reads in 87.680 CPU sec, 2.775 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283102, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 334, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (352.46, 165.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 109, #reads = 1081575 mem = 536882449 peak = 1183358976
[M::process] read 679070 sequences (100000179 bp) in 2.052 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283159, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 334, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (352.29, 166.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678244 sequences (100000286 bp) in 1.430 CPU sec, 1.426 real sec...
[M::mem_process_seqs] Processed 678472 reads in 138.859 CPU sec, 4.416 real sec
[M::mem_process_seqs] Processed 678726 reads in 85.925 CPU sec, 2.721 real sec
sorting block 110, #reads = 1080175 mem = 536881453 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283531, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (349.16, 165.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678414 sequences (100000046 bp) in 2.273 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282189, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (355.15, 165.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677796 sequences (100000059 bp) in 1.390 CPU sec, 1.385 real sec...
[M::mem_process_seqs] Processed 679070 reads in 139.020 CPU sec, 4.415 real sec
[M::mem_process_seqs] Processed 678244 reads in 86.538 CPU sec, 2.741 real sec
sorting block 111, #reads = 1080505 mem = 536877326 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283274, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (348.57, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677482 sequences (100000118 bp) in 1.972 CPU sec, 2.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282718, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (352.33, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677268 sequences (100000106 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_process_seqs] Processed 678414 reads in 139.144 CPU sec, 4.429 real sec
[M::mem_process_seqs] Processed 677796 reads in 86.493 CPU sec, 2.737 real sec
sorting block 112, #reads = 1079793 mem = 536877732 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282783, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 340, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (354.93, 162.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676774 sequences (100000024 bp) in 2.333 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283070, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (354.01, 161.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676620 sequences (100000168 bp) in 1.390 CPU sec, 1.388 real sec...
[M::mem_process_seqs] Processed 677482 reads in 140.824 CPU sec, 4.471 real sec
sorting block 113, #reads = 1079821 mem = 536880941 peak = 1183358976
[M::mem_process_seqs] Processed 677268 reads in 88.387 CPU sec, 2.799 real sec
sorting block 114, #reads = 1078989 mem = 536879673 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283016, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (355.88, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676936 sequences (100000035 bp) in 1.975 CPU sec, 2.070 real sec...
[M::process] read 676842 sequences (100000138 bp) in 1.390 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282350, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (357.55, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676774 reads in 138.607 CPU sec, 4.409 real sec
[M::mem_process_seqs] Processed 676620 reads in 86.746 CPU sec, 2.750 real sec
sorting block 115, #reads = 1078690 mem = 536877197 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282490, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (356.36, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676508 sequences (100000099 bp) in 2.327 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282612, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 342, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (357.27, 163.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676608 sequences (100000009 bp) in 1.390 CPU sec, 1.388 real sec...
[M::mem_process_seqs] Processed 676936 reads in 139.827 CPU sec, 4.443 real sec
[M::mem_process_seqs] Processed 676842 reads in 87.955 CPU sec, 2.785 real sec
sorting block 116, #reads = 1078118 mem = 536878295 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282118, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (358.80, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676644 sequences (100000238 bp) in 2.012 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282608, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 342, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (358.71, 164.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676628 sequences (100000264 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 676508 reads in 140.043 CPU sec, 4.461 real sec
[M::mem_process_seqs] Processed 676608 reads in 87.790 CPU sec, 2.773 real sec
sorting block 117, #reads = 1078114 mem = 536877104 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282097, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (360.91, 166.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684226 sequences (100000117 bp) in 2.146 CPU sec, 2.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282490, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 348, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (363.77, 167.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685138 sequences (100000116 bp) in 1.430 CPU sec, 1.437 real sec...
[M::mem_process_seqs] Processed 676644 reads in 141.162 CPU sec, 4.493 real sec
sorting block 118, #reads = 1078375 mem = 536878258 peak = 1183358976
[M::mem_process_seqs] Processed 676628 reads in 89.271 CPU sec, 2.831 real sec
sorting block 119, #reads = 1077976 mem = 536878577 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284998, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (318.73, 155.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685524 sequences (100000154 bp) in 2.033 CPU sec, 2.118 real sec...
[M::process] read 684818 sequences (100000128 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285491, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (310.20, 150.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684226 reads in 149.946 CPU sec, 4.770 real sec
[M::mem_process_seqs] Processed 685138 reads in 92.982 CPU sec, 2.952 real sec
sorting block 120, #reads = 1079047 mem = 536879122 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285452, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (307.49, 147.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683030 sequences (100000064 bp) in 2.273 CPU sec, 2.363 real sec...
[M::process] read 682962 sequences (100000161 bp) in 1.430 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285441, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 287, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (310.09, 147.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685524 reads in 153.094 CPU sec, 4.866 real sec
[M::mem_process_seqs] Processed 684818 reads in 95.654 CPU sec, 3.037 real sec
sorting block 121, #reads = 1077583 mem = 536880946 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284781, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (320.66, 152.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682388 sequences (100000090 bp) in 2.048 CPU sec, 2.156 real sec...
[M::process] read 681238 sequences (100000003 bp) in 1.420 CPU sec, 1.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285047, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 300, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (321.35, 152.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683030 reads in 147.613 CPU sec, 4.699 real sec
[M::mem_process_seqs] Processed 682962 reads in 91.953 CPU sec, 2.913 real sec
sorting block 122, #reads = 1077028 mem = 536879910 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284142, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 304, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (326.12, 155.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680976 sequences (100000059 bp) in 2.123 CPU sec, 2.228 real sec...
[M::process] read 680578 sequences (100000197 bp) in 1.420 CPU sec, 1.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284876, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (331.88, 156.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682388 reads in 147.766 CPU sec, 4.703 real sec
sorting block 123, #reads = 1079051 mem = 536878083 peak = 1183358976
[M::mem_process_seqs] Processed 681238 reads in 91.249 CPU sec, 2.895 real sec
sorting block 124, #reads = 1077878 mem = 536877850 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283776, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 316, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (334.79, 158.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680526 sequences (100000231 bp) in 2.052 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284052, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (337.74, 159.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680180 sequences (100000123 bp) in 1.430 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 680976 reads in 141.788 CPU sec, 4.512 real sec
[M::mem_process_seqs] Processed 680578 reads in 88.385 CPU sec, 2.801 real sec
sorting block 125, #reads = 1079515 mem = 536881277 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283745, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (337.36, 158.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680334 sequences (100000031 bp) in 2.126 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284396, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (339.78, 159.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679920 sequences (100000086 bp) in 1.410 CPU sec, 1.414 real sec...
[M::mem_process_seqs] Processed 680526 reads in 146.965 CPU sec, 4.683 real sec
[M::mem_process_seqs] Processed 680180 reads in 95.932 CPU sec, 3.040 real sec
sorting block 126, #reads = 1078844 mem = 536880794 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284547, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (338.25, 158.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679836 sequences (100000122 bp) in 2.055 CPU sec, 2.157 real sec...
[M::process] read 679630 sequences (100000279 bp) in 1.400 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283618, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 323, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (339.61, 158.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680334 reads in 143.364 CPU sec, 4.560 real sec
sorting block 127, #reads = 1075475 mem = 536878992 peak = 1183358976
[M::mem_process_seqs] Processed 679920 reads in 90.086 CPU sec, 2.858 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283733, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 323, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (340.18, 158.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 128, #reads = 1078042 mem = 536878667 peak = 1183358976
[M::process] read 679106 sequences (100000179 bp) in 2.167 CPU sec, 2.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283155, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (340.24, 159.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678994 sequences (100000146 bp) in 1.430 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 679836 reads in 142.735 CPU sec, 4.545 real sec
[M::mem_process_seqs] Processed 679630 reads in 90.286 CPU sec, 2.854 real sec
sorting block 129, #reads = 1079299 mem = 536877279 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283726, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (346.11, 160.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679230 sequences (100000026 bp) in 2.320 CPU sec, 2.449 real sec...
[M::process] read 679036 sequences (100000116 bp) in 1.380 CPU sec, 1.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282635, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (345.34, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (281, 325, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (193, 413)
[M::mem_pestat] mean and std.dev: (305.44, 21.86)
[M::mem_pestat] low and high boundaries for proper pairs: (149, 457)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 679106 reads in 144.302 CPU sec, 4.587 real sec
[M::mem_process_seqs] Processed 678994 reads in 91.436 CPU sec, 2.901 real sec
sorting block 130, #reads = 1077815 mem = 536879408 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283391, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (344.69, 161.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678390 sequences (100000071 bp) in 2.172 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283128, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (345.88, 161.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677728 sequences (100000240 bp) in 1.390 CPU sec, 1.391 real sec...
[M::mem_process_seqs] Processed 679230 reads in 141.385 CPU sec, 4.495 real sec
[M::mem_process_seqs] Processed 679036 reads in 89.431 CPU sec, 2.833 real sec
sorting block 131, #reads = 1078448 mem = 536883728 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283226, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (351.68, 163.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677366 sequences (100000104 bp) in 2.409 CPU sec, 2.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282405, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (356.10, 164.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676956 sequences (100000296 bp) in 1.370 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 678390 reads in 140.422 CPU sec, 4.460 real sec
sorting block 132, #reads = 1079268 mem = 536879051 peak = 1183358976
[M::mem_process_seqs] Processed 677728 reads in 87.698 CPU sec, 2.777 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282605, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (359.21, 165.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 133, #reads = 1078889 mem = 536880007 peak = 1183358976
[M::process] read 677130 sequences (100000250 bp) in 2.382 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282417, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (359.76, 165.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677272 sequences (100000135 bp) in 1.410 CPU sec, 1.404 real sec...
[M::mem_process_seqs] Processed 677366 reads in 141.096 CPU sec, 4.483 real sec
[M::mem_process_seqs] Processed 676956 reads in 89.610 CPU sec, 2.836 real sec
sorting block 134, #reads = 1080123 mem = 536878800 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282678, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (359.31, 166.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677208 sequences (100000018 bp) in 2.244 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282631, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677228 sequences (100000069 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (223, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (360.39, 169.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677130 reads in 139.795 CPU sec, 4.443 real sec
[M::mem_process_seqs] Processed 677272 reads in 87.322 CPU sec, 2.765 real sec
sorting block 135, #reads = 1078750 mem = 536879863 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282537, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (359.70, 169.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677172 sequences (100000115 bp) in 2.308 CPU sec, 2.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282498, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 342, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (359.55, 167.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677978 sequences (100000146 bp) in 1.430 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 677208 reads in 141.026 CPU sec, 4.481 real sec
[M::mem_process_seqs] Processed 677228 reads in 89.465 CPU sec, 2.836 real sec
sorting block 136, #reads = 1078829 mem = 536879901 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282617, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (360.35, 169.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677338 sequences (100000036 bp) in 2.388 CPU sec, 2.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283475, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 332, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (354.58, 170.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681340 sequences (100000001 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_process_seqs] Processed 677172 reads in 142.730 CPU sec, 4.531 real sec
sorting block 137, #reads = 1078280 mem = 536878362 peak = 1183358976
[M::mem_process_seqs] Processed 677978 reads in 90.634 CPU sec, 2.871 real sec
sorting block 138, #reads = 1078395 mem = 536878629 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282558, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 341, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (361.50, 171.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683550 sequences (100000237 bp) in 2.301 CPU sec, 2.369 real sec...
[M::process] read 683726 sequences (100000299 bp) in 1.440 CPU sec, 1.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283891, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 310, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (333.66, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677338 reads in 155.865 CPU sec, 4.958 real sec
[M::mem_process_seqs] Processed 681340 reads in 99.992 CPU sec, 3.178 real sec
sorting block 139, #reads = 1071587 mem = 536878160 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285019, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (321.46, 156.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682476 sequences (100000145 bp) in 2.260 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285020, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 295, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (319.76, 155.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681558 sequences (100000150 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 683550 reads in 140.237 CPU sec, 4.457 real sec
[M::mem_process_seqs] Processed 683726 reads in 88.251 CPU sec, 2.798 real sec
sorting block 140, #reads = 1079470 mem = 536879671 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284469, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 304, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (327.61, 158.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681158 sequences (100000024 bp) in 2.120 CPU sec, 2.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284001, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 309, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (330.95, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680850 sequences (100000187 bp) in 1.410 CPU sec, 1.415 real sec...
[M::mem_process_seqs] Processed 682476 reads in 137.486 CPU sec, 4.373 real sec
[M::mem_process_seqs] Processed 681558 reads in 85.858 CPU sec, 2.717 real sec
sorting block 141, #reads = 1081958 mem = 536879146 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283665, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (333.36, 159.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680766 sequences (100000182 bp) in 2.116 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283663, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (336.55, 160.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681158 reads in 135.356 CPU sec, 4.311 real sec
[M::process] read 680254 sequences (100000244 bp) in 1.380 CPU sec, 1.387 real sec...
sorting block 142, #reads = 1082777 mem = 536879283 peak = 1183358976
[M::mem_process_seqs] Processed 680850 reads in 85.082 CPU sec, 2.694 real sec
sorting block 143, #reads = 1082728 mem = 536879098 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283980, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (336.46, 160.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680022 sequences (100000143 bp) in 2.348 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283588, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (338.58, 160.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679762 sequences (100000218 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_process_seqs] Processed 680766 reads in 135.099 CPU sec, 4.292 real sec
[M::mem_process_seqs] Processed 680254 reads in 84.169 CPU sec, 2.662 real sec
sorting block 144, #reads = 1083402 mem = 536879269 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283862, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 323, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (342.34, 161.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679390 sequences (100000229 bp) in 2.401 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283107, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (344.68, 162.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679518 sequences (100000079 bp) in 1.400 CPU sec, 1.400 real sec...
[M::mem_process_seqs] Processed 680022 reads in 135.075 CPU sec, 4.290 real sec
[M::mem_process_seqs] Processed 679762 reads in 84.685 CPU sec, 2.680 real sec
sorting block 145, #reads = 1082823 mem = 536879907 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283723, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (345.25, 162.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679318 sequences (100000204 bp) in 2.473 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284068, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (344.36, 161.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679390 reads in 134.913 CPU sec, 4.286 real sec
[M::process] read 679514 sequences (100000157 bp) in 1.483 CPU sec, 1.489 real sec...
[M::mem_process_seqs] Processed 679518 reads in 84.438 CPU sec, 2.671 real sec
sorting block 146, #reads = 1082656 mem = 536878444 peak = 1183358976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283121, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (346.95, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679040 sequences (100000138 bp) in 2.312 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283149, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (345.22, 162.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678604 sequences (100000299 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 679318 reads in 137.299 CPU sec, 4.363 real sec
sorting block 147, #reads = 1082279 mem = 536876853 peak = 1183358976
[M::mem_process_seqs] Processed 679514 reads in 86.415 CPU sec, 2.734 real sec
sorting block 148, #reads = 1080213 mem = 536878616 peak = 1210060800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283904, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (349.02, 163.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678598 sequences (100000020 bp) in 2.391 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283299, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (349.82, 163.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678460 sequences (100000165 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_process_seqs] Processed 679040 reads in 136.890 CPU sec, 4.349 real sec
[M::mem_process_seqs] Processed 678604 reads in 86.191 CPU sec, 2.725 real sec
sorting block 149, #reads = 1081144 mem = 536881395 peak = 1210060800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283329, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (352.01, 164.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678322 sequences (100000279 bp) in 2.317 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282622, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (353.03, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678286 sequences (100000101 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 678598 reads in 137.635 CPU sec, 4.370 real sec
[M::mem_process_seqs] Processed 678460 reads in 86.352 CPU sec, 2.738 real sec
sorting block 150, #reads = 1081005 mem = 536878627 peak = 1210060800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282953, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (352.59, 164.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677916 sequences (100000011 bp) in 2.379 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283054, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (352.28, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677996 sequences (100000100 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_process_seqs] Processed 678322 reads in 139.137 CPU sec, 4.417 real sec
sorting block 151, #reads = 1080473 mem = 536877552 peak = 1210060800
[M::mem_process_seqs] Processed 678286 reads in 87.387 CPU sec, 2.763 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283086, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (351.91, 162.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 152, #reads = 1079871 mem = 536878170 peak = 1210060800
[M::process] read 677844 sequences (100000097 bp) in 2.327 CPU sec, 2.416 real sec...
[M::process] read 677636 sequences (100000193 bp) in 1.390 CPU sec, 1.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283003, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (349.84, 161.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677916 reads in 141.447 CPU sec, 4.493 real sec
[M::mem_process_seqs] Processed 677996 reads in 88.997 CPU sec, 2.819 real sec
sorting block 153, #reads = 1079065 mem = 536881146 peak = 1210060800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283035, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (349.91, 160.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677914 sequences (100000296 bp) in 2.102 CPU sec, 2.157 real sec...
[M::process] read 678128 sequences (100000081 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282984, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (350.44, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677844 reads in 140.418 CPU sec, 4.464 real sec
[M::mem_process_seqs] Processed 677636 reads in 87.967 CPU sec, 2.787 real sec
sorting block 154, #reads = 1079093 mem = 536878251 peak = 1210060800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282316, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (350.06, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678150 sequences (100000113 bp) in 2.011 CPU sec, 2.097 real sec...
[M::process] read 678088 sequences (100000273 bp) in 1.400 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283500, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (348.57, 161.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677914 reads in 142.537 CPU sec, 4.538 real sec
[M::mem_process_seqs] Processed 678128 reads in 89.320 CPU sec, 2.834 real sec
sorting block 155, #reads = 1078379 mem = 536877783 peak = 1210060800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282811, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.02, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678122 sequences (100000141 bp) in 2.158 CPU sec, 2.235 real sec...
[M::process] read 677768 sequences (100000129 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283517, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.40, 164.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678150 reads in 141.912 CPU sec, 4.510 real sec
sorting block 156, #reads = 1078228 mem = 536880583 peak = 1210060800
[M::mem_process_seqs] Processed 678088 reads in 88.634 CPU sec, 2.813 real sec
sorting block 157, #reads = 1078834 mem = 536876914 peak = 1210060800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283069, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (351.65, 164.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677996 sequences (100000292 bp) in 2.326 CPU sec, 2.399 real sec...
[M::process] read 677954 sequences (100000266 bp) in 1.410 CPU sec, 1.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283047, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (353.22, 165.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678122 reads in 142.418 CPU sec, 4.523 real sec
[M::mem_process_seqs] Processed 677768 reads in 88.761 CPU sec, 2.816 real sec
sorting block 158, #reads = 1078490 mem = 536879927 peak = 1210060800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282800, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (353.54, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677906 sequences (100000181 bp) in 2.225 CPU sec, 2.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282844, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (354.61, 167.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686930 sequences (100000131 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 677996 reads in 147.855 CPU sec, 4.704 real sec
[M::mem_process_seqs] Processed 677954 reads in 95.064 CPU sec, 3.011 real sec
sorting block 159, #reads = 1077881 mem = 536877328 peak = 1210060800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282663, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (355.16, 168.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686150 sequences (100000061 bp) in 2.240 CPU sec, 2.315 real sec...
[M::process] read 685698 sequences (100000091 bp) in 1.440 CPU sec, 1.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286848, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 276, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (303.44, 149.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677906 reads in 160.660 CPU sec, 5.115 real sec
[M::mem_process_seqs] Processed 686930 reads in 97.241 CPU sec, 3.090 real sec
sorting block 160, #reads = 1071413 mem = 536880321 peak = 1210060800
[M::process] read 684544 sequences (100000272 bp) in 2.456 CPU sec, 2.520 real sec...
[M::process] read 683412 sequences (100000248 bp) in 1.440 CPU sec, 1.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285931, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (313.79, 150.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (718, 2367, 2367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5665)
[M::mem_pestat] mean and std.dev: (1833.90, 826.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7314)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 684544 reads in 79.131 CPU sec, 2.702 real sec
sorting block 161, #reads = 1074949 mem = 536877782 peak = 1210060800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285862, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (308.55, 150.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685698 reads in 163.894 CPU sec, 5.771 real sec
sorting block 162, #reads = 1075899 mem = 536879259 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286677, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (307.63, 150.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683032 sequences (100000174 bp) in 2.277 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285507, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (320.55, 153.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686150 reads in 315.141 CPU sec, 10.586 real sec
[M::mem_process_seqs] Processed 683412 reads in 110.713 CPU sec, 3.506 real sec
[M::process] read 682134 sequences (100000144 bp) in 1.870 CPU sec, 1.939 real sec...
sorting block 163, #reads = 1078856 mem = 536879195 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284980, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 301, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (323.31, 154.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681426 sequences (100000300 bp) in 2.069 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284847, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 307, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (327.52, 155.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680860 sequences (100000029 bp) in 1.390 CPU sec, 1.393 real sec...
[M::mem_process_seqs] Processed 683032 reads in 147.393 CPU sec, 4.686 real sec
[M::mem_process_seqs] Processed 682134 reads in 92.377 CPU sec, 2.927 real sec
sorting block 164, #reads = 1078966 mem = 536878014 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284414, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 312, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (331.33, 156.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680564 sequences (100000213 bp) in 2.467 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284514, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (335.21, 158.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680100 sequences (100000025 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_process_seqs] Processed 681426 reads in 144.715 CPU sec, 4.596 real sec
[M::mem_process_seqs] Processed 680860 reads in 90.728 CPU sec, 2.875 real sec
sorting block 165, #reads = 1078085 mem = 536881519 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284020, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (336.54, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679528 sequences (100000040 bp) in 2.200 CPU sec, 2.325 real sec...
[M::process] read 679648 sequences (100000044 bp) in 1.410 CPU sec, 1.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283845, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (339.25, 159.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680564 reads in 145.810 CPU sec, 4.636 real sec
sorting block 166, #reads = 1079303 mem = 536878731 peak = 1212952576
[M::mem_process_seqs] Processed 680100 reads in 91.290 CPU sec, 2.892 real sec
sorting block 167, #reads = 1077988 mem = 536880311 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283862, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (343.39, 160.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679614 sequences (100000003 bp) in 2.234 CPU sec, 2.346 real sec...
[M::process] read 679544 sequences (100000278 bp) in 1.420 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283687, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (343.84, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679528 reads in 145.098 CPU sec, 4.609 real sec
[M::mem_process_seqs] Processed 679648 reads in 90.873 CPU sec, 2.883 real sec
sorting block 168, #reads = 1077273 mem = 536879270 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284317, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (344.66, 162.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679074 sequences (100000181 bp) in 2.381 CPU sec, 2.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283806, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 326, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (345.28, 162.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679056 sequences (100000271 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_process_seqs] Processed 679614 reads in 145.464 CPU sec, 4.620 real sec
[M::mem_process_seqs] Processed 679544 reads in 92.057 CPU sec, 2.915 real sec
sorting block 169, #reads = 1077645 mem = 536880408 peak = 1212952576
[M::process] read 679220 sequences (100000285 bp) in 2.334 CPU sec, 2.485 real sec...
[M::process] read 678798 sequences (100000060 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283453, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (343.72, 161.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679056 reads in 78.038 CPU sec, 2.462 real sec
sorting block 170, #reads = 1077645 mem = 536878339 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283905, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (345.37, 162.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283685, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 327, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (346.53, 163.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678924 sequences (100000214 bp) in 1.708 CPU sec, 1.711 real sec...
[M::mem_process_seqs] Processed 679074 reads in 222.332 CPU sec, 7.051 real sec
[M::mem_process_seqs] Processed 679220 reads in 103.576 CPU sec, 3.309 real sec
sorting block 171, #reads = 1077993 mem = 536880374 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283298, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (346.89, 163.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679208 sequences (100000208 bp) in 2.342 CPU sec, 2.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283296, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (345.46, 163.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678780 sequences (100000206 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_process_seqs] Processed 678798 reads in 144.019 CPU sec, 4.579 real sec
[M::mem_process_seqs] Processed 678924 reads in 91.190 CPU sec, 2.886 real sec
sorting block 172, #reads = 1079473 mem = 536881226 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283254, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (344.82, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678352 sequences (100000107 bp) in 2.066 CPU sec, 2.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282765, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 330, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (349.97, 165.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678468 sequences (100000074 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_process_seqs] Processed 679208 reads in 142.984 CPU sec, 4.549 real sec
sorting block 173, #reads = 1077650 mem = 536879892 peak = 1212952576
[M::mem_process_seqs] Processed 678780 reads in 89.873 CPU sec, 2.849 real sec
sorting block 174, #reads = 1079207 mem = 536879261 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283136, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 329, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (349.57, 165.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678744 sequences (100000250 bp) in 2.179 CPU sec, 2.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283140, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (348.40, 163.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678286 sequences (100000095 bp) in 1.390 CPU sec, 1.394 real sec...
[M::mem_process_seqs] Processed 678352 reads in 139.896 CPU sec, 4.448 real sec
[M::mem_process_seqs] Processed 678468 reads in 87.414 CPU sec, 2.770 real sec
sorting block 175, #reads = 1080598 mem = 536878092 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282667, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (347.54, 163.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678084 sequences (100000134 bp) in 1.996 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282956, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 330, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (350.58, 165.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677838 sequences (100000170 bp) in 1.400 CPU sec, 1.408 real sec...
[M::mem_process_seqs] Processed 678744 reads in 138.121 CPU sec, 4.399 real sec
[M::mem_process_seqs] Processed 678286 reads in 86.841 CPU sec, 2.746 real sec
sorting block 176, #reads = 1080141 mem = 536879117 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283173, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (352.02, 166.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677840 sequences (100000123 bp) in 2.206 CPU sec, 2.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282768, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (353.51, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677900 sequences (100000110 bp) in 1.380 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 678084 reads in 137.808 CPU sec, 4.378 real sec
[M::mem_process_seqs] Processed 677838 reads in 85.624 CPU sec, 2.713 real sec
sorting block 177, #reads = 1080812 mem = 536880978 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283163, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (355.08, 167.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677842 sequences (100000036 bp) in 2.018 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282864, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (356.15, 169.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 632368 sequences (93244349 bp) in 1.360 CPU sec, 1.359 real sec...
[M::mem_process_seqs] Processed 677840 reads in 136.540 CPU sec, 4.346 real sec
sorting block 178, #reads = 1080756 mem = 536880158 peak = 1212952576
[M::mem_process_seqs] Processed 677900 reads in 85.648 CPU sec, 2.714 real sec
sorting block 179, #reads = 1079922 mem = 536878836 peak = 1212952576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283014, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (358.71, 171.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 262977, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (353.54, 168.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677842 reads in 132.449 CPU sec, 4.305 real sec
[M::mem_process_seqs] Processed 632368 reads in 82.952 CPU sec, 2.690 real sec
sorting block 180, #reads = 1080498 mem = 536880535 peak = 1212952576
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 195456486
overall: 70002565120 mem 22413.249 user 523.249 sys 745.764 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cVw-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 745.798 sec; CPU: 22936.501 sec
sorting block 181, #reads = 515904 mem = 257578828 peak = 1212952576
uniq header count 1
merging 182 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 10407876006
output reads: 196296836
bam_mem_sort: 182 calls 67.725 user 0.480 sys 68.679 real
bam_write: 182 calls 111.375 user 2.186 sys 114.513 real
execute: 1 calls 35.652 user 1.703 sys 805.126 real
merge_files: 1 calls 35.643 user 1.671 sys 58.606 real
parse_chunk: 2904 calls 88.234 user 26.772 sys 117.284 real
read_chunk: 7823 calls 75.697 user 69.144 sys 709.014 real
sort_block: 1 calls 56.404 user 68.462 sys 681.780 real
write_chunk: 4917 calls 35.289 user 1.419 sys 57.738 real
overall: 3277328384 mem 2269.704 user 112.384 sys 805.147 real
ls: cannot access '*.cram': No such file or directory