cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_5TB-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1694.373 CPU sec, 63.367 real sec
[M::process] read 700108 sequences (100000097 bp) in 1.233 CPU sec, 1.237 real sec...
[M::process] read 698898 sequences (100000041 bp) in 1.406 CPU sec, 1.470 real sec...
[M::process] read 697338 sequences (100000182 bp) in 1.189 CPU sec, 1.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 286250, 24, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 265, 762)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1960)
[M::mem_pestat] mean and std.dev: (449.71, 382.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2559)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 238, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (262.43, 130.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (315, 558, 1234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3072)
[M::mem_pestat] mean and std.dev: (714.21, 572.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3991)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 335, 1298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3376)
[M::mem_pestat] mean and std.dev: (527.88, 482.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4415)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696174 sequences (100000112 bp) in 1.149 CPU sec, 1.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 285935, 14, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 234, 735)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1851)
[M::mem_pestat] mean and std.dev: (345.08, 356.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2409)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (271.29, 135.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (384, 805, 1944)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5064)
[M::mem_pestat] mean and std.dev: (946.93, 674.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6624)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 327, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1255)
[M::mem_pestat] mean and std.dev: (339.98, 236.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1622)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 286744, 19, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 296, 1099)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2987)
[M::mem_pestat] mean and std.dev: (521.61, 583.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3931)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (265.52, 132.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 560, 1008)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2694)
[M::mem_pestat] mean and std.dev: (606.37, 503.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3537)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 276, 593)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441)
[M::mem_pestat] mean and std.dev: (305.96, 224.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1865)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700108 reads in 217.832 CPU sec, 6.998 real sec
[M::mem_process_seqs] Processed 697338 reads in 146.030 CPU sec, 4.727 real sec
[M::mem_process_seqs] Processed 698898 reads in 159.111 CPU sec, 5.168 real sec
sorting block 0, #reads = 1085567 mem = 536879712 peak = 581492736
[M::process] read 695360 sequences (100000248 bp) in 1.900 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 285257, 6, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 346, 757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913)
[M::mem_pestat] mean and std.dev: (439.70, 357.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2491)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 250, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (275.10, 137.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 356, 983)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2451)
[M::mem_pestat] mean and std.dev: (629.00, 549.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3185)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 695040 sequences (100000142 bp) in 1.386 CPU sec, 1.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 284643, 21, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 296, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (329.16, 209.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 252, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (276.60, 137.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (507, 968, 2375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6111)
[M::mem_pestat] mean and std.dev: (1480.05, 1331.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7979)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 264, 562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324)
[M::mem_pestat] mean and std.dev: (379.06, 302.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1705)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696174 reads in 137.109 CPU sec, 4.368 real sec
sorting block 1, #reads = 1084042 mem = 536880376 peak = 785965056
[M::process] read 695150 sequences (100000144 bp) in 1.219 CPU sec, 1.237 real sec...
[M::mem_process_seqs] Processed 695360 reads in 87.932 CPU sec, 2.778 real sec
sorting block 2, #reads = 1082767 mem = 536880218 peak = 894742528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284641, 11, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 280, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (271.79, 113.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (274.89, 135.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (590, 786, 971)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1733)
[M::mem_pestat] mean and std.dev: (740.20, 202.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2114)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 251, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (280.53, 168.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694620 sequences (100000197 bp) in 2.072 CPU sec, 2.206 real sec...
[M::process] read 693602 sequences (100000079 bp) in 1.350 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285102, 8, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 271, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (240.61, 112.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (275.04, 136.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281)
[M::mem_pestat] mean and std.dev: (319.85, 201.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1651)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695040 reads in 137.402 CPU sec, 4.364 real sec
[M::mem_process_seqs] Processed 695150 reads in 87.651 CPU sec, 2.787 real sec
sorting block 3, #reads = 1081008 mem = 536876905 peak = 894742528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 284801, 24, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 235, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1556)
[M::mem_pestat] mean and std.dev: (330.73, 260.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2027)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 253, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.65, 136.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (330, 596, 1395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3525)
[M::mem_pestat] mean and std.dev: (927.79, 845.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4590)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 367, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (370.43, 231.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1370)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 692590 sequences (100000213 bp) in 2.388 CPU sec, 2.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284701, 18, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 261, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334)
[M::mem_pestat] mean and std.dev: (344.50, 263.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1723)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (279.83, 137.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (621, 1356, 3069)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7965)
[M::mem_pestat] mean and std.dev: (1901.82, 1943.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10413)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 342, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (315.10, 153.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695100 sequences (100000196 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 694620 reads in 135.287 CPU sec, 4.293 real sec
[M::mem_process_seqs] Processed 693602 reads in 86.204 CPU sec, 2.731 real sec
sorting block 4, #reads = 1082410 mem = 536879178 peak = 894742528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 283910, 16, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 303, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (307.33, 152.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 260, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (282.91, 138.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (432, 462, 1618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3990)
[M::mem_pestat] mean and std.dev: (841.75, 784.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5176)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 353, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (351.10, 193.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700072 sequences (100000196 bp) in 2.248 CPU sec, 2.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284895, 9, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 333, 744)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1858)
[M::mem_pestat] mean and std.dev: (377.36, 320.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2415)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (277.61, 137.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 278, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (281.18, 167.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698512 sequences (100000240 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_process_seqs] Processed 692590 reads in 135.317 CPU sec, 4.308 real sec
sorting block 5, #reads = 1081943 mem = 536877296 peak = 894742528
[M::mem_process_seqs] Processed 695100 reads in 87.272 CPU sec, 2.771 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 286669, 18, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 258, 1140)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120)
[M::mem_pestat] mean and std.dev: (488.28, 682.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4110)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 239, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (265.15, 133.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (340, 1263, 2704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7432)
[M::mem_pestat] mean and std.dev: (1462.53, 1455.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9796)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 322, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (306.63, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 6, #reads = 1080720 mem = 536881958 peak = 894742528
[M::process] read 697660 sequences (100000193 bp) in 2.611 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 286477, 23, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 266, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (328.79, 217.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (269.24, 135.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (456, 501, 645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 1023)
[M::mem_pestat] mean and std.dev: (414.00, 192.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 308, 677)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1641)
[M::mem_pestat] mean and std.dev: (395.07, 312.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2123)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700072 reads in 132.545 CPU sec, 4.201 real sec
[M::mem_process_seqs] Processed 698512 reads in 85.445 CPU sec, 2.688 real sec
[M::process] read 696146 sequences (100000027 bp) in 1.999 CPU sec, 2.063 real sec...
sorting block 7, #reads = 1086746 mem = 536879018 peak = 894742528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285873, 14, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 631, 980)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2534)
[M::mem_pestat] mean and std.dev: (721.50, 680.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3442)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 247, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (273.34, 138.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 51, 1084)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3150)
[M::mem_pestat] mean and std.dev: (521.92, 829.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4183)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 464, 776)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852)
[M::mem_pestat] mean and std.dev: (497.96, 361.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2390)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695928 sequences (100000031 bp) in 1.923 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285438, 21, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 476, 835)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2019)
[M::mem_pestat] mean and std.dev: (432.50, 301.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2611)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (277.50, 139.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (93, 291, 712)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1950)
[M::mem_pestat] mean and std.dev: (323.89, 286.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2569)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 319, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416)
[M::mem_pestat] mean and std.dev: (347.74, 232.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1831)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697660 reads in 131.720 CPU sec, 4.193 real sec
[M::process] read 695840 sequences (100000023 bp) in 1.436 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 696146 reads in 86.052 CPU sec, 2.719 real sec
sorting block 8, #reads = 1085366 mem = 536886202 peak = 894742528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285191, 8, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 207, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (245.87, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (276.86, 139.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 319, 729)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1799)
[M::mem_pestat] mean and std.dev: (394.14, 283.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2334)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 695520 sequences (100000157 bp) in 2.296 CPU sec, 2.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285025, 15, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 227, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (261.00, 146.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (274.26, 136.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (486, 1446, 2387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6189)
[M::mem_pestat] mean and std.dev: (1080.69, 817.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8090)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 284, 686)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1610)
[M::mem_pestat] mean and std.dev: (399.67, 252.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2072)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694722 sequences (100000071 bp) in 1.400 CPU sec, 1.390 real sec...
[M::mem_process_seqs] Processed 695928 reads in 136.335 CPU sec, 4.337 real sec
sorting block 9, #reads = 1083582 mem = 536881685 peak = 894742528
[M::mem_process_seqs] Processed 695840 reads in 88.647 CPU sec, 2.804 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 285260, 16, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 267, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623)
[M::mem_pestat] mean and std.dev: (315.19, 245.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2108)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (273.65, 135.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 481, 1705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4625)
[M::mem_pestat] mean and std.dev: (1052.07, 1344.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6432)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 264, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253)
[M::mem_pestat] mean and std.dev: (322.56, 183.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1600)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 693670 sequences (100000030 bp) in 2.077 CPU sec, 2.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285059, 25, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 198, 682)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1790)
[M::mem_pestat] mean and std.dev: (318.14, 291.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2344)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 252, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (275.55, 135.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 1318, 2275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6437)
[M::mem_pestat] mean and std.dev: (1364.36, 1219.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8518)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 357, 634)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1508)
[M::mem_pestat] mean and std.dev: (400.13, 257.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1945)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 693522 sequences (100000194 bp) in 1.400 CPU sec, 1.392 real sec...
[M::mem_process_seqs] Processed 695520 reads in 137.073 CPU sec, 4.369 real sec
sorting block 10, #reads = 1083080 mem = 536879173 peak = 894742528
[M::mem_process_seqs] Processed 694722 reads in 88.588 CPU sec, 2.810 real sec
sorting block 11, #reads = 1082077 mem = 536878344 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284171, 11, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 462, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1694)
[M::mem_pestat] mean and std.dev: (471.02, 311.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2174)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 255, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (279.19, 137.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 398, 1077)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2773)
[M::mem_pestat] mean and std.dev: (601.64, 481.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3621)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 295, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1341)
[M::mem_pestat] mean and std.dev: (346.30, 261.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1737)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698788 sequences (100000107 bp) in 2.314 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 284344, 21, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 298, 719)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857)
[M::mem_pestat] mean and std.dev: (351.00, 330.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2426)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 257, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (281.37, 139.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (460, 1878, 3061)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8263)
[M::mem_pestat] mean and std.dev: (1931.71, 1509.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10864)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411)
[M::mem_pestat] mean and std.dev: (379.95, 235.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1805)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700654 sequences (100000097 bp) in 1.400 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 693670 reads in 138.111 CPU sec, 4.390 real sec
[M::mem_process_seqs] Processed 693522 reads in 88.639 CPU sec, 2.809 real sec
sorting block 12, #reads = 1081573 mem = 536880775 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 286447, 16, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 281, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (230.92, 102.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (268.61, 135.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 714, 8594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25274)
[M::mem_pestat] mean and std.dev: (2584.25, 3498.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 33614)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 343, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1922)
[M::mem_pestat] mean and std.dev: (427.15, 358.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2496)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698610 sequences (100000260 bp) in 2.444 CPU sec, 2.603 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 286764, 15, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 400, 1166)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3018)
[M::mem_pestat] mean and std.dev: (549.48, 562.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3944)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 237, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (262.94, 131.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 760, 3202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9098)
[M::mem_pestat] mean and std.dev: (1840.20, 2036.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12046)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 371, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240)
[M::mem_pestat] mean and std.dev: (392.56, 257.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1580)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698788 reads in 137.256 CPU sec, 4.371 real sec
[M::mem_process_seqs] Processed 700654 reads in 89.677 CPU sec, 2.825 real sec
[M::process] read 697486 sequences (100000117 bp) in 2.161 CPU sec, 2.267 real sec...
sorting block 13, #reads = 1081657 mem = 536878028 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 285909, 23, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 225, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080)
[M::mem_pestat] mean and std.dev: (271.63, 159.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1384)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (267.74, 133.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (306, 353, 1235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3093)
[M::mem_pestat] mean and std.dev: (743.32, 745.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4022)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 265, 616)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528)
[M::mem_pestat] mean and std.dev: (387.69, 336.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1984)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696392 sequences (100000300 bp) in 2.215 CPU sec, 2.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 285949, 19, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 254, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (272.52, 136.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (271.89, 135.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (265, 568, 1410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3700)
[M::mem_pestat] mean and std.dev: (836.74, 844.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4845)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 245, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (260.43, 153.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698610 reads in 138.851 CPU sec, 4.433 real sec
sorting block 14, #reads = 1086189 mem = 536883267 peak = 955334656
[M::process] read 695702 sequences (100000146 bp) in 1.590 CPU sec, 1.621 real sec...
[M::mem_process_seqs] Processed 697486 reads in 94.385 CPU sec, 2.988 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284963, 28, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 468, 800)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012)
[M::mem_pestat] mean and std.dev: (520.50, 458.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2618)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 251, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (275.28, 137.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (319, 588, 1982)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5308)
[M::mem_pestat] mean and std.dev: (965.70, 1038.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6971)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 337, 798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976)
[M::mem_pestat] mean and std.dev: (429.20, 319.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2565)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 15, #reads = 1085218 mem = 536881303 peak = 955334656
[M::process] read 695732 sequences (100000206 bp) in 2.468 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284854, 24, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 216, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (209.65, 90.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 252, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (276.07, 137.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (490, 1198, 1835)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4525)
[M::mem_pestat] mean and std.dev: (1254.54, 980.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5870)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 354, 736)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1762)
[M::mem_pestat] mean and std.dev: (417.81, 324.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2275)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696392 reads in 139.601 CPU sec, 4.468 real sec
[M::mem_process_seqs] Processed 695702 reads in 91.005 CPU sec, 2.878 real sec
[M::process] read 695156 sequences (100000251 bp) in 2.459 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 284915, 15, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 285, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498)
[M::mem_pestat] mean and std.dev: (352.31, 313.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1943)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (274.04, 135.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (614, 856, 2604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6584)
[M::mem_pestat] mean and std.dev: (1466.80, 1574.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8574)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (258.28, 176.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 16, #reads = 1083625 mem = 536880075 peak = 955334656
[M::process] read 694404 sequences (100000104 bp) in 2.065 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285306, 22, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 405, 1752)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4844)
[M::mem_pestat] mean and std.dev: (606.84, 725.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6390)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (275.04, 135.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 402, 758)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1874)
[M::mem_pestat] mean and std.dev: (314.82, 195.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2432)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 375, 706)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1676)
[M::mem_pestat] mean and std.dev: (427.44, 260.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2161)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695732 reads in 136.896 CPU sec, 4.373 real sec
[M::mem_process_seqs] Processed 695156 reads in 88.775 CPU sec, 2.804 real sec
[M::process] read 694374 sequences (100000242 bp) in 1.976 CPU sec, 2.063 real sec...
sorting block 17, #reads = 1083126 mem = 536880529 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284378, 17, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 245, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (251.00, 136.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 252, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (275.74, 135.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (249, 691, 1722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4668)
[M::mem_pestat] mean and std.dev: (788.06, 624.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6141)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208)
[M::mem_pestat] mean and std.dev: (298.93, 185.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1546)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694988 sequences (100000283 bp) in 2.186 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 284847, 19, 66)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 536, 2353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6633)
[M::mem_pestat] mean and std.dev: (1423.87, 1770.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8773)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (278.37, 138.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (387, 1001, 1728)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4410)
[M::mem_pestat] mean and std.dev: (1181.58, 950.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5751)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 271, 625)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1543)
[M::mem_pestat] mean and std.dev: (329.49, 275.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2002)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694404 reads in 134.772 CPU sec, 4.303 real sec
sorting block 18, #reads = 1082357 mem = 536877931 peak = 955334656
[M::process] read 700450 sequences (100000063 bp) in 1.894 CPU sec, 2.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284575, 22, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 339, 683)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665)
[M::mem_pestat] mean and std.dev: (345.74, 277.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2156)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (275.46, 136.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 996, 1320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3686)
[M::mem_pestat] mean and std.dev: (997.05, 933.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4869)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 311, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (349.44, 214.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694988 reads in 79.342 CPU sec, 2.498 real sec
[M::process] read 699450 sequences (100000149 bp) in 1.754 CPU sec, 1.764 real sec...
sorting block 19, #reads = 1079228 mem = 536880636 peak = 955334656
[M::mem_process_seqs] Processed 694374 reads in 206.120 CPU sec, 6.515 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286978, 24, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 284, 954)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2446)
[M::mem_pestat] mean and std.dev: (394.24, 435.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3192)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 239, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (263.99, 132.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 438, 1325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3469)
[M::mem_pestat] mean and std.dev: (635.57, 753.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4541)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 356, 687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1587)
[M::mem_pestat] mean and std.dev: (441.33, 263.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2037)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 286735, 19, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 228, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455)
[M::mem_pestat] mean and std.dev: (344.86, 307.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1876)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (266.90, 133.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (296, 808, 2018)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5462)
[M::mem_pestat] mean and std.dev: (1478.89, 1757.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8511)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 245, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (261.09, 137.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697972 sequences (100000135 bp) in 2.536 CPU sec, 2.690 real sec...
[M::mem_process_seqs] Processed 700450 reads in 147.588 CPU sec, 4.678 real sec
[M::mem_process_seqs] Processed 699450 reads in 86.125 CPU sec, 2.721 real sec
sorting block 20, #reads = 1083102 mem = 536878614 peak = 955334656
[M::process] read 697176 sequences (100000002 bp) in 2.184 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 286057, 26, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 326, 1665)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4519)
[M::mem_pestat] mean and std.dev: (910.60, 1310.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6152)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (271.03, 135.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 358, 1786)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5264)
[M::mem_pestat] mean and std.dev: (828.28, 946.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7003)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 301, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277)
[M::mem_pestat] mean and std.dev: (279.71, 124.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1628)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 21, #reads = 1087184 mem = 536877369 peak = 955334656
[M::process] read 696452 sequences (100000204 bp) in 1.853 CPU sec, 1.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 285460, 12, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 272, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (313.23, 199.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (273.47, 136.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (459, 518, 988)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2046)
[M::mem_pestat] mean and std.dev: (520.73, 318.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2575)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 347, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224)
[M::mem_pestat] mean and std.dev: (349.74, 236.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1573)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697972 reads in 135.201 CPU sec, 4.318 real sec
[M::mem_process_seqs] Processed 697176 reads in 88.941 CPU sec, 2.800 real sec
[M::process] read 697146 sequences (100000278 bp) in 2.001 CPU sec, 2.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285087, 16, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 238, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (275.79, 146.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 251, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (275.60, 137.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (416, 835, 3147)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8609)
[M::mem_pestat] mean and std.dev: (1045.29, 990.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11340)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 285, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160)
[M::mem_pestat] mean and std.dev: (301.34, 148.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1475)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1084813 mem = 536879323 peak = 955334656
[M::process] read 695782 sequences (100000071 bp) in 2.249 CPU sec, 2.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285958, 12, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 445, 1048)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2732)
[M::mem_pestat] mean and std.dev: (670.26, 632.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3574)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (269.47, 134.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 459, 1750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5000)
[M::mem_pestat] mean and std.dev: (735.33, 752.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6625)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 249, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (277.62, 211.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1330)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696452 reads in 136.603 CPU sec, 4.365 real sec
[M::mem_process_seqs] Processed 697146 reads in 90.818 CPU sec, 2.867 real sec
[M::process] read 695342 sequences (100000232 bp) in 1.976 CPU sec, 2.097 real sec...
sorting block 23, #reads = 1083654 mem = 536877882 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284550, 18, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 294, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (281.41, 168.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1373)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (271.71, 133.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (940, 1356, 5972)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16036)
[M::mem_pestat] mean and std.dev: (3015.33, 2858.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21068)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 286, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1575)
[M::mem_pestat] mean and std.dev: (351.43, 230.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2038)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694566 sequences (100000210 bp) in 2.082 CPU sec, 2.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284318, 20, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 531, 1148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3078)
[M::mem_pestat] mean and std.dev: (656.68, 675.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4043)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 249, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (271.75, 132.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (366, 1072, 2010)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5298)
[M::mem_pestat] mean and std.dev: (932.06, 814.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6942)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 293, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (296.96, 129.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695782 reads in 137.272 CPU sec, 4.361 real sec
[M::mem_process_seqs] Processed 695342 reads in 89.409 CPU sec, 2.815 real sec
[M::process] read 694168 sequences (100000057 bp) in 1.584 CPU sec, 1.627 real sec...
sorting block 24, #reads = 1082494 mem = 536877812 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284795, 20, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 226, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1459)
[M::mem_pestat] mean and std.dev: (321.94, 315.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1886)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.75, 132.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (305, 652, 3266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9188)
[M::mem_pestat] mean and std.dev: (1344.25, 1451.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12149)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 267, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188)
[M::mem_pestat] mean and std.dev: (296.30, 202.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1523)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700462 sequences (100000053 bp) in 2.152 CPU sec, 2.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283819, 31, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 219, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (244.64, 127.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 254, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (277.37, 136.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (685, 1618, 2449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5977)
[M::mem_pestat] mean and std.dev: (1479.61, 990.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7741)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 407, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1806)
[M::mem_pestat] mean and std.dev: (418.41, 266.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2329)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694566 reads in 133.370 CPU sec, 4.250 real sec
sorting block 25, #reads = 1080966 mem = 536877905 peak = 955334656
[M::mem_process_seqs] Processed 694168 reads in 87.876 CPU sec, 2.769 real sec
[M::process] read 702600 sequences (100000184 bp) in 1.713 CPU sec, 1.768 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 286806, 13, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 202, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (290.67, 201.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1412)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 234, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.18, 131.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 1189, 2541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7183)
[M::mem_pestat] mean and std.dev: (1370.46, 1231.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9504)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 378, 692)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640)
[M::mem_pestat] mean and std.dev: (452.96, 363.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2114)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 26, #reads = 1081149 mem = 536881111 peak = 955334656
[M::process] read 699676 sequences (100000249 bp) in 2.324 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 287786, 15, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 250, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253)
[M::mem_pestat] mean and std.dev: (282.35, 176.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1617)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 229, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (256.73, 130.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 266, 1051)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2677)
[M::mem_pestat] mean and std.dev: (594.13, 584.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3490)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 210, 837)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153)
[M::mem_pestat] mean and std.dev: (476.91, 480.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2811)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700462 reads in 139.842 CPU sec, 4.448 real sec
[M::mem_process_seqs] Processed 702600 reads in 91.054 CPU sec, 2.867 real sec
[M::process] read 697958 sequences (100000276 bp) in 1.808 CPU sec, 1.847 real sec...
sorting block 27, #reads = 1081654 mem = 536880739 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 286776, 23, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (228.73, 119.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 906)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 238, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (265.34, 134.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (361, 1216, 3158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8752)
[M::mem_pestat] mean and std.dev: (1538.39, 1367.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11549)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 304, 629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507)
[M::mem_pestat] mean and std.dev: (436.28, 343.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1946)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697294 sequences (100000002 bp) in 2.391 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 286173, 21, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 273, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096)
[M::mem_pestat] mean and std.dev: (304.54, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1388)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (269.37, 136.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (724, 839, 3420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8812)
[M::mem_pestat] mean and std.dev: (2146.81, 2375.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11648)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 363, 1411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3853)
[M::mem_pestat] mean and std.dev: (551.49, 584.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5074)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699676 reads in 138.259 CPU sec, 4.407 real sec
[M::mem_process_seqs] Processed 697958 reads in 92.421 CPU sec, 2.911 real sec
[M::process] read 696504 sequences (100000161 bp) in 1.961 CPU sec, 2.050 real sec...
sorting block 28, #reads = 1084673 mem = 536878306 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 285505, 27, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 300, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292)
[M::mem_pestat] mean and std.dev: (301.10, 199.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (272.32, 137.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 433, 906)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2516)
[M::mem_pestat] mean and std.dev: (622.11, 578.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3321)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 208, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (254.07, 137.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695710 sequences (100000200 bp) in 2.412 CPU sec, 2.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284892, 19, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 288, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (268.73, 139.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (274.22, 137.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 299, 1849)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5071)
[M::mem_pestat] mean and std.dev: (803.06, 1009.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6682)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 425, 652)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1580)
[M::mem_pestat] mean and std.dev: (426.83, 335.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2044)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697294 reads in 142.373 CPU sec, 4.532 real sec
[M::mem_process_seqs] Processed 696504 reads in 91.760 CPU sec, 2.894 real sec
sorting block 29, #reads = 1083819 mem = 536880422 peak = 955334656
[M::process] read 695734 sequences (100000031 bp) in 2.281 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285235, 16, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 270, 1070)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2846)
[M::mem_pestat] mean and std.dev: (511.33, 504.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3734)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (273.23, 135.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 1393, 1800)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5034)
[M::mem_pestat] mean and std.dev: (1094.67, 1212.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6651)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 290, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (299.95, 155.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 30, #reads = 1081307 mem = 536880481 peak = 955334656
[M::process] read 694758 sequences (100000187 bp) in 2.104 CPU sec, 2.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285280, 19, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 256, 672)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1648)
[M::mem_pestat] mean and std.dev: (358.79, 337.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2136)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (274.26, 136.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (419, 944, 2161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5645)
[M::mem_pestat] mean and std.dev: (1160.68, 1022.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7387)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 228, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (252.16, 125.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695710 reads in 140.094 CPU sec, 4.466 real sec
[M::mem_process_seqs] Processed 695734 reads in 92.692 CPU sec, 2.924 real sec
[M::process] read 694764 sequences (100000016 bp) in 1.842 CPU sec, 1.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 284688, 19, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 296, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271)
[M::mem_pestat] mean and std.dev: (331.63, 223.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1633)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (275.72, 136.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 867, 2085)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5623)
[M::mem_pestat] mean and std.dev: (1257.26, 1315.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7392)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 319, 704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1704)
[M::mem_pestat] mean and std.dev: (451.40, 361.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2204)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 31, #reads = 1080526 mem = 536879351 peak = 955334656
[M::process] read 701954 sequences (100000200 bp) in 2.362 CPU sec, 2.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 284781, 20, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 308, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (306.86, 155.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 253, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (277.41, 137.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (463, 1289, 2473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6493)
[M::mem_pestat] mean and std.dev: (1237.94, 1025.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8503)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 283, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216)
[M::mem_pestat] mean and std.dev: (343.78, 245.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1561)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694758 reads in 139.799 CPU sec, 4.449 real sec
[M::mem_process_seqs] Processed 694764 reads in 91.382 CPU sec, 2.876 real sec
[M::process] read 706168 sequences (100000301 bp) in 1.757 CPU sec, 1.794 real sec...
sorting block 32, #reads = 1078980 mem = 536881996 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 287466, 14, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 274, 1009)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2731)
[M::mem_pestat] mean and std.dev: (450.79, 554.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3592)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 229, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (255.79, 128.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 494, 968)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2680)
[M::mem_pestat] mean and std.dev: (537.69, 493.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3536)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 367, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1896)
[M::mem_pestat] mean and std.dev: (447.89, 309.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2449)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 702798 sequences (100000090 bp) in 2.626 CPU sec, 2.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 289566, 10, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 324, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (293.00, 158.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1518)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (245.02, 122.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (446, 1033, 1539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3725)
[M::mem_pestat] mean and std.dev: (1072.20, 753.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4818)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 267, 762)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898)
[M::mem_pestat] mean and std.dev: (386.09, 378.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2466)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701954 reads in 147.135 CPU sec, 4.679 real sec
[M::mem_process_seqs] Processed 706168 reads in 97.040 CPU sec, 3.052 real sec
sorting block 33, #reads = 1078608 mem = 536876490 peak = 955334656
[M::process] read 701272 sequences (100000042 bp) in 2.409 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 288273, 17, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 349, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (326.89, 189.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 228, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.41, 127.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 291, 1605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4639)
[M::mem_pestat] mean and std.dev: (831.41, 1024.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6156)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 243, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156)
[M::mem_pestat] mean and std.dev: (319.61, 235.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1491)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699876 sequences (100000219 bp) in 2.346 CPU sec, 2.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 287397, 18, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 598, 1310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3508)
[M::mem_pestat] mean and std.dev: (810.51, 862.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 233, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (259.78, 130.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (542, 962, 1299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2813)
[M::mem_pestat] mean and std.dev: (883.71, 461.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3570)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 268, 728)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792)
[M::mem_pestat] mean and std.dev: (467.66, 439.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2324)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702798 reads in 145.982 CPU sec, 4.650 real sec
sorting block 34, #reads = 1082847 mem = 536877528 peak = 955334656
[M::mem_process_seqs] Processed 701272 reads in 93.239 CPU sec, 2.948 real sec
[M::process] read 698212 sequences (100000036 bp) in 2.205 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 287202, 15, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 266, 836)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192)
[M::mem_pestat] mean and std.dev: (413.67, 406.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2870)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 238, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (263.75, 132.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (685, 1050, 3767)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9931)
[M::mem_pestat] mean and std.dev: (2161.20, 2471.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13013)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 350, 936)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2414)
[M::mem_pestat] mean and std.dev: (535.14, 574.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3153)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 35, #reads = 1082536 mem = 536883410 peak = 955334656
[M::process] read 696630 sequences (100000093 bp) in 2.363 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286366, 18, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 274, 1037)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2699)
[M::mem_pestat] mean and std.dev: (433.24, 369.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3530)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (269.12, 135.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (326, 1166, 1413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587)
[M::mem_pestat] mean and std.dev: (1079.22, 914.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4735)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 230, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346)
[M::mem_pestat] mean and std.dev: (330.57, 270.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1746)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699876 reads in 144.816 CPU sec, 4.608 real sec
[M::mem_process_seqs] Processed 698212 reads in 95.820 CPU sec, 3.014 real sec
[M::process] read 696166 sequences (100000189 bp) in 1.707 CPU sec, 1.772 real sec...
sorting block 36, #reads = 1081400 mem = 536879572 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285562, 18, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 382, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486)
[M::mem_pestat] mean and std.dev: (406.69, 273.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1905)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (271.75, 135.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (370, 515, 1187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2821)
[M::mem_pestat] mean and std.dev: (868.35, 777.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3979)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 349, 705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623)
[M::mem_pestat] mean and std.dev: (435.62, 305.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2082)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695004 sequences (100000009 bp) in 2.702 CPU sec, 2.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 285639, 17, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 287, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (291.70, 170.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (273.38, 136.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 780, 1421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3913)
[M::mem_pestat] mean and std.dev: (679.31, 539.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5159)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 280, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (333.33, 202.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694816 sequences (100000112 bp) in 1.590 CPU sec, 1.592 real sec...
[M::mem_process_seqs] Processed 696630 reads in 161.187 CPU sec, 5.123 real sec
[M::mem_process_seqs] Processed 696166 reads in 108.201 CPU sec, 3.424 real sec
sorting block 37, #reads = 1080159 mem = 536882101 peak = 955334656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 285035, 12, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 236, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081)
[M::mem_pestat] mean and std.dev: (273.51, 183.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1389)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 252, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (275.88, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (537, 868, 1884)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4578)
[M::mem_pestat] mean and std.dev: (959.82, 687.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5925)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 272, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1392)
[M::mem_pestat] mean and std.dev: (322.77, 262.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1799)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 705024 sequences (100000134 bp) in 2.473 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284863, 21, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 282, 840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210)
[M::mem_pestat] mean and std.dev: (481.80, 424.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2895)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (276.81, 138.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 519, 3745)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11145)
[M::mem_pestat] mean and std.dev: (1554.38, 1940.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14845)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 312, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (310.08, 183.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695004 reads in 161.189 CPU sec, 5.119 real sec
[M::process] read 702836 sequences (100000177 bp) in 1.920 CPU sec, 1.921 real sec...
sorting block 38, #reads = 1079808 mem = 536884288 peak = 955334656
[M::mem_process_seqs] Processed 694816 reads in 109.242 CPU sec, 3.448 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 288647, 11, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 284, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124)
[M::mem_pestat] mean and std.dev: (295.43, 217.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1448)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 222, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.64, 124.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (840, 984, 2617)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6171)
[M::mem_pestat] mean and std.dev: (989.22, 683.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7948)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 277, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1471)
[M::mem_pestat] mean and std.dev: (385.77, 315.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1896)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 39, #reads = 1079702 mem = 536878598 peak = 974024704
[M::process] read 700254 sequences (100000097 bp) in 2.649 CPU sec, 2.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 288166, 13, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 297, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (256.85, 83.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 229, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (255.50, 128.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (435, 1406, 2966)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8028)
[M::mem_pestat] mean and std.dev: (1804.31, 1712.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10559)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 400, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515)
[M::mem_pestat] mean and std.dev: (436.40, 312.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1954)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699152 sequences (100000125 bp) in 1.740 CPU sec, 1.743 real sec...
[M::mem_process_seqs] Processed 705024 reads in 159.247 CPU sec, 5.064 real sec
[M::mem_process_seqs] Processed 702836 reads in 101.410 CPU sec, 3.205 real sec
sorting block 40, #reads = 1084792 mem = 536881348 peak = 974024704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 287321, 15, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 227, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (254.76, 149.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 236, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (261.87, 130.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 906)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (271, 424, 1029)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545)
[M::mem_pestat] mean and std.dev: (656.27, 535.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3303)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 221, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (266.65, 196.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698794 sequences (100000018 bp) in 2.473 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286910, 17, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 309, 893)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2197)
[M::mem_pestat] mean and std.dev: (521.15, 455.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2849)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 240, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (264.86, 131.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 1484, 2554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7256)
[M::mem_pestat] mean and std.dev: (1524.69, 1335.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9607)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 291, 975)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2585)
[M::mem_pestat] mean and std.dev: (544.58, 566.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3390)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700254 reads in 155.723 CPU sec, 5.202 real sec
[M::process] read 698418 sequences (100000146 bp) in 2.073 CPU sec, 2.102 real sec...
[M::mem_process_seqs] Processed 699152 reads in 103.780 CPU sec, 3.270 real sec
sorting block 41, #reads = 1086515 mem = 536885608 peak = 974024704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286521, 15, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 230, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (298.74, 193.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (265.99, 132.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 1001, 1620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4228)
[M::mem_pestat] mean and std.dev: (1112.13, 893.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5532)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 249, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (276.33, 156.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697178 sequences (100000220 bp) in 2.884 CPU sec, 3.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286406, 21, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 428, 875)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329)
[M::mem_pestat] mean and std.dev: (539.68, 536.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3056)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (266.02, 131.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (475, 1680, 2698)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7144)
[M::mem_pestat] mean and std.dev: (1482.95, 1137.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9367)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 313, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (396.40, 253.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1489)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698794 reads in 156.323 CPU sec, 4.963 real sec
[M::mem_process_seqs] Processed 698418 reads in 104.144 CPU sec, 3.279 real sec
sorting block 42, #reads = 1083791 mem = 536880217 peak = 974024704
[M::process] read 696170 sequences (100000067 bp) in 2.300 CPU sec, 2.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285538, 7, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 374, 1174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3142)
[M::mem_pestat] mean and std.dev: (671.38, 748.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4126)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (271.13, 135.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 320, 676)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1566)
[M::mem_pestat] mean and std.dev: (433.02, 310.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2011)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 43, #reads = 1083076 mem = 536879042 peak = 974024704
[M::process] read 695652 sequences (100000063 bp) in 2.336 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 284963, 20, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 335, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209)
[M::mem_pestat] mean and std.dev: (322.73, 170.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1544)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (271.44, 134.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 515, 941)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2387)
[M::mem_pestat] mean and std.dev: (493.53, 347.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3110)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 362, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183)
[M::mem_pestat] mean and std.dev: (313.86, 178.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1511)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697178 reads in 165.950 CPU sec, 5.289 real sec
[M::process] read 695718 sequences (100000061 bp) in 1.833 CPU sec, 1.833 real sec...
[M::mem_process_seqs] Processed 696170 reads in 110.705 CPU sec, 3.502 real sec
sorting block 44, #reads = 1079890 mem = 536878337 peak = 974024704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284811, 13, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 289, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373)
[M::mem_pestat] mean and std.dev: (400.75, 359.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1837)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (273.74, 136.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (444, 506, 4670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13122)
[M::mem_pestat] mean and std.dev: (1608.46, 1776.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17348)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 280, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327)
[M::mem_pestat] mean and std.dev: (393.51, 300.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1708)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 708156 sequences (100000149 bp) in 2.803 CPU sec, 2.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284391, 20, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 211, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (215.67, 103.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (273.87, 135.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (408, 904, 2479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6621)
[M::mem_pestat] mean and std.dev: (1136.47, 1012.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8692)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 264, 1328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3686)
[M::mem_pestat] mean and std.dev: (624.57, 787.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4865)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695652 reads in 176.339 CPU sec, 5.618 real sec
[M::process] read 705158 sequences (100000009 bp) in 2.095 CPU sec, 2.104 real sec...
[M::mem_process_seqs] Processed 695718 reads in 121.432 CPU sec, 3.840 real sec
sorting block 45, #reads = 1077871 mem = 536879600 peak = 974024704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 289474, 20, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 963)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2475)
[M::mem_pestat] mean and std.dev: (525.98, 527.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3231)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (239.98, 119.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (320, 655, 1682)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4406)
[M::mem_pestat] mean and std.dev: (870.89, 778.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5768)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 271, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (323.12, 199.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 702858 sequences (100000288 bp) in 2.434 CPU sec, 2.676 real sec...
[M::process] read 701430 sequences (100000251 bp) in 1.490 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 288576, 17, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 379, 1228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302)
[M::mem_pestat] mean and std.dev: (540.98, 474.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4339)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 222, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.48, 123.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 681, 902)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368)
[M::mem_pestat] mean and std.dev: (469.21, 339.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3101)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 401, 1191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3093)
[M::mem_pestat] mean and std.dev: (702.66, 671.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4044)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 708156 reads in 173.755 CPU sec, 5.551 real sec
[M::mem_process_seqs] Processed 705158 reads in 106.731 CPU sec, 3.383 real sec
sorting block 46, #reads = 1074390 mem = 536881751 peak = 974024704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 288260, 28, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 302, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (275.96, 160.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 229, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (255.21, 127.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 526, 1231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3259)
[M::mem_pestat] mean and std.dev: (735.04, 836.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4273)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 314, 690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1684)
[M::mem_pestat] mean and std.dev: (368.46, 289.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2181)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 47, #reads = 1082383 mem = 536880888 peak = 974024704
[M::process] read 700808 sequences (100000130 bp) in 2.701 CPU sec, 2.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 286847, 18, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 346, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (277.47, 138.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1363)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 234, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (260.28, 130.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (547, 769, 1850)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4456)
[M::mem_pestat] mean and std.dev: (1035.22, 780.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5759)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657)
[M::mem_pestat] mean and std.dev: (382.90, 266.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2145)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697800 sequences (100000143 bp) in 1.531 CPU sec, 1.533 real sec...
[M::mem_process_seqs] Processed 702858 reads in 165.961 CPU sec, 5.338 real sec
[M::mem_process_seqs] Processed 701430 reads in 110.042 CPU sec, 3.504 real sec
sorting block 48, #reads = 1080431 mem = 536879434 peak = 974024704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 287153, 21, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 355, 662)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626)
[M::mem_pestat] mean and std.dev: (343.65, 256.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2108)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 234, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (260.62, 130.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 302, 2823)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8113)
[M::mem_pestat] mean and std.dev: (1326.14, 1831.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10758)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 324, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280)
[M::mem_pestat] mean and std.dev: (421.35, 312.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1670)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696082 sequences (100000153 bp) in 2.532 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285824, 25, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 204, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (192.72, 77.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (266.45, 131.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 579, 982)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2534)
[M::mem_pestat] mean and std.dev: (557.00, 414.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3310)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 263, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256)
[M::mem_pestat] mean and std.dev: (343.15, 273.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1620)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695314 sequences (100000057 bp) in 1.668 CPU sec, 1.666 real sec...
[M::mem_process_seqs] Processed 700808 reads in 157.964 CPU sec, 5.007 real sec
[M::mem_process_seqs] Processed 697800 reads in 104.634 CPU sec, 3.305 real sec
sorting block 49, #reads = 1080237 mem = 536877803 peak = 974024704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284869, 19, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 358, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (332.87, 189.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1342)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 246, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (269.91, 132.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (570, 665, 2089)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5127)
[M::mem_pestat] mean and std.dev: (1091.78, 920.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6646)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 215, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270)
[M::mem_pestat] mean and std.dev: (288.83, 221.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1646)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694476 sequences (100000291 bp) in 2.744 CPU sec, 2.902 real sec...
[M::process] read 497030 sequences (71609096 bp) in 1.200 CPU sec, 1.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284881, 11, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 340, 730)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1834)
[M::mem_pestat] mean and std.dev: (418.66, 295.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2386)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 248, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (270.28, 131.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (294, 379, 1560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4092)
[M::mem_pestat] mean and std.dev: (668.09, 642.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5358)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 276, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (274.59, 177.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696082 reads in 156.404 CPU sec, 4.977 real sec
sorting block 50, #reads = 1077820 mem = 536880222 peak = 974024704
[M::mem_process_seqs] Processed 695314 reads in 104.283 CPU sec, 3.318 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285005, 28, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1555)
[M::mem_pestat] mean and std.dev: (370.71, 347.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2019)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 252, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (275.53, 135.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (714, 936, 1989)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4539)
[M::mem_pestat] mean and std.dev: (1269.11, 995.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5814)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 350, 815)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2079)
[M::mem_pestat] mean and std.dev: (423.78, 362.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2711)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 203581, 26, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 268, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (214.89, 102.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (278.06, 137.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (308, 514, 2035)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5489)
[M::mem_pestat] mean and std.dev: (629.33, 636.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7216)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 319, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (311.33, 159.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1334)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694476 reads in 135.327 CPU sec, 4.626 real sec
sorting block 51, #reads = 1078646 mem = 536881714 peak = 974024704
[M::mem_process_seqs] Processed 497030 reads in 75.747 CPU sec, 2.448 real sec
sorting block 52, #reads = 1079205 mem = 536880763 peak = 1014878208
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 57003026
overall: 70000451584 mem 7923.935 user 325.409 sys 282.663 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_5TB-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1136_5TB-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_5TB-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1136_5TB-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1136_5TB-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 282.701 sec; CPU: 8249.347 sec
sorting block 53, #reads = 25917 mem = 12995972 peak = 1014878208
uniq header count 1
merging 54 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3327101988
output reads: 57376626
bam_mem_sort: 54 calls 28.077 user 0.333 sys 28.503 real
bam_write: 54 calls 12.951 user 0.858 sys 14.744 real
execute: 1 calls 16.324 user 1.879 sys 305.745 real
merge_files: 1 calls 16.302 user 1.857 sys 23.002 real
parse_chunk: 849 calls 35.228 user 8.646 sys 44.606 real
read_chunk: 2295 calls 19.685 user 18.192 sys 222.640 real
sort_block: 1 calls 12.418 user 17.566 sys 211.118 real
write_chunk: 1444 calls 15.897 user 1.581 sys 22.013 real
overall: 2761773056 mem 822.862 user 37.898 sys 305.770 real
ls: cannot access '*.cram': No such file or directory