cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1311.071 CPU sec, 882.797 real sec
[M::process] read 702806 sequences (100000021 bp) in 0.989 CPU sec, 0.992 real sec...
[M::process] read 701540 sequences (100000264 bp) in 1.260 CPU sec, 1.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291867, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.73, 114.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700738 sequences (100000288 bp) in 1.290 CPU sec, 1.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291723, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.97, 115.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699606 sequences (100000057 bp) in 1.225 CPU sec, 1.265 real sec...
[M::mem_process_seqs] Processed 702806 reads in 122.099 CPU sec, 3.884 real sec
[M::mem_process_seqs] Processed 701540 reads in 77.649 CPU sec, 2.504 real sec
sorting block 0, #reads = 1095602 mem = 536879295 peak = 588599296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291920, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 1264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3472)
[M::mem_pestat] mean and std.dev: (570.27, 592.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4576)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.48, 117.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699058 sequences (100000251 bp) in 1.656 CPU sec, 1.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291274, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.96, 118.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 376, 717)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741)
[M::mem_pestat] mean and std.dev: (404.38, 225.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2253)
[M::mem_pestat] skip orientation RR
[M::process] read 697954 sequences (100000200 bp) in 1.343 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 700738 reads in 115.087 CPU sec, 3.655 real sec
[M::mem_process_seqs] Processed 699606 reads in 75.980 CPU sec, 2.425 real sec
sorting block 1, #reads = 1094865 mem = 536877310 peak = 719257600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291226, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.03, 119.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698068 sequences (100000067 bp) in 2.287 CPU sec, 2.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290886, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.55, 121.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699058 reads in 117.119 CPU sec, 3.744 real sec
[M::mem_process_seqs] Processed 697954 reads in 78.017 CPU sec, 2.481 real sec
sorting block 2, #reads = 1094478 mem = 536878100 peak = 876670976
[M::process] read 697792 sequences (100000295 bp) in 1.776 CPU sec, 1.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290587, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.14, 120.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697888 sequences (100000232 bp) in 2.282 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290745, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.44, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698068 reads in 119.610 CPU sec, 3.815 real sec
[M::mem_process_seqs] Processed 697792 reads in 78.904 CPU sec, 2.503 real sec
sorting block 3, #reads = 1093602 mem = 536880086 peak = 876670976
[M::process] read 697044 sequences (100000099 bp) in 1.849 CPU sec, 1.914 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291081, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 295, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (296.50, 104.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.42, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 4, #reads = 1092926 mem = 536877132 peak = 925233152
[M::process] read 696556 sequences (100000081 bp) in 1.742 CPU sec, 1.811 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290439, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.37, 121.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 117, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (149.44, 54.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 629)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697888 reads in 121.528 CPU sec, 3.867 real sec
[M::mem_process_seqs] Processed 697044 reads in 79.205 CPU sec, 2.506 real sec
[M::process] read 696604 sequences (100000299 bp) in 1.650 CPU sec, 1.697 real sec...
sorting block 5, #reads = 1093237 mem = 536878349 peak = 925233152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290273, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.29, 119.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 357, 945)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2275)
[M::mem_pestat] mean and std.dev: (433.56, 268.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2940)
[M::mem_pestat] skip orientation RR
[M::process] read 696534 sequences (100000080 bp) in 2.359 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290255, 5, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (252.51, 118.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 255, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (332.60, 135.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696556 reads in 121.328 CPU sec, 3.878 real sec
[M::mem_process_seqs] Processed 696604 reads in 79.847 CPU sec, 2.532 real sec
sorting block 6, #reads = 1091901 mem = 536877478 peak = 925233152
[M::process] read 697014 sequences (100000163 bp) in 2.018 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289801, 11, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 174, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (204.78, 136.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1412)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (253.22, 119.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 484, 553)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1369)
[M::mem_pestat] mean and std.dev: (416.91, 354.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1833)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 696730 sequences (100000003 bp) in 2.072 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290424, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (250.81, 118.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696534 reads in 123.362 CPU sec, 3.936 real sec
[M::mem_process_seqs] Processed 697014 reads in 81.163 CPU sec, 2.572 real sec
sorting block 7, #reads = 1092751 mem = 536879493 peak = 925233152
[M::process] read 696674 sequences (100000003 bp) in 2.287 CPU sec, 2.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289879, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.51, 118.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290759, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.08, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696730 reads in 124.398 CPU sec, 3.994 real sec
sorting block 8, #reads = 1091091 mem = 536877504 peak = 925233152
[M::process] read 696436 sequences (100000215 bp) in 2.566 CPU sec, 2.750 real sec...
[M::mem_process_seqs] Processed 696674 reads in 81.142 CPU sec, 2.586 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290160, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 197, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240)
[M::mem_pestat] mean and std.dev: (321.09, 184.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1603)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.62, 120.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696794 sequences (100000064 bp) in 2.140 CPU sec, 2.287 real sec...
[M::mem_process_seqs] Processed 696436 reads in 70.479 CPU sec, 2.321 real sec
sorting block 9, #reads = 1090992 mem = 536887541 peak = 934133760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290364, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.16, 120.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702340 sequences (100000280 bp) in 2.170 CPU sec, 2.326 real sec...
[M::mem_process_seqs] Processed 696794 reads in 70.320 CPU sec, 2.315 real sec
sorting block 10, #reads = 1091201 mem = 536881756 peak = 978669568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292074, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (240.69, 114.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702340 reads in 68.372 CPU sec, 2.239 real sec
[M::process] read 700876 sequences (100000119 bp) in 2.367 CPU sec, 2.605 real sec...
sorting block 11, #reads = 1093998 mem = 536879628 peak = 978669568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291131, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.08, 116.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699874 sequences (100000234 bp) in 1.964 CPU sec, 2.056 real sec...
[M::mem_process_seqs] Processed 700876 reads in 68.059 CPU sec, 2.206 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291506, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (245.98, 116.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (346, 858, 1233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3007)
[M::mem_pestat] mean and std.dev: (960.20, 820.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4241)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699874 reads in 67.489 CPU sec, 2.192 real sec
[M::process] read 699252 sequences (100000105 bp) in 2.458 CPU sec, 2.673 real sec...
sorting block 12, #reads = 1096445 mem = 536876886 peak = 978669568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291371, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.18, 117.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 834, 1364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3670)
[M::mem_pestat] mean and std.dev: (770.18, 537.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4823)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699252 reads in 67.269 CPU sec, 2.188 real sec
[M::process] read 698966 sequences (100000248 bp) in 2.786 CPU sec, 2.999 real sec...
sorting block 13, #reads = 1095142 mem = 536880826 peak = 978669568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291180, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (249.79, 118.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698966 reads in 68.701 CPU sec, 2.230 real sec
[M::process] read 698626 sequences (100000080 bp) in 2.165 CPU sec, 2.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291038, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.29, 119.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698626 reads in 68.223 CPU sec, 2.224 real sec
sorting block 14, #reads = 1094952 mem = 536880728 peak = 978669568
[M::process] read 698258 sequences (100000136 bp) in 2.732 CPU sec, 3.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290905, 10, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 387, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (304.38, 135.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.81, 120.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 548, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859)
[M::mem_pestat] mean and std.dev: (297.00, 310.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2467)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 698112 sequences (100000146 bp) in 1.790 CPU sec, 1.925 real sec...
[M::mem_process_seqs] Processed 698258 reads in 65.596 CPU sec, 2.116 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291019, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.48, 120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1094406 mem = 536878573 peak = 978669568
[M::mem_process_seqs] Processed 698112 reads in 64.549 CPU sec, 2.090 real sec
[M::process] read 697584 sequences (100000083 bp) in 2.368 CPU sec, 2.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290576, 15, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.51, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 288, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125)
[M::mem_pestat] mean and std.dev: (254.31, 151.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1462)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 697036 sequences (100000252 bp) in 1.711 CPU sec, 1.774 real sec...
[M::mem_process_seqs] Processed 697584 reads in 65.277 CPU sec, 2.113 real sec
sorting block 16, #reads = 1094245 mem = 536881930 peak = 978669568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290562, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.14, 119.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696902 sequences (100000248 bp) in 2.237 CPU sec, 2.437 real sec...
[M::mem_process_seqs] Processed 697036 reads in 66.046 CPU sec, 2.153 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 289971, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.20, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696902 reads in 66.393 CPU sec, 2.159 real sec
sorting block 17, #reads = 1093559 mem = 536877763 peak = 978669568
[M::process] read 696622 sequences (100000123 bp) in 2.409 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290701, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 190, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 373)
[M::mem_pestat] mean and std.dev: (173.78, 39.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 447)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.40, 120.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697410 sequences (100000108 bp) in 1.781 CPU sec, 1.851 real sec...
[M::mem_process_seqs] Processed 696622 reads in 65.477 CPU sec, 2.119 real sec
sorting block 18, #reads = 1092550 mem = 536882535 peak = 978669568
[M::process] read 696640 sequences (100000188 bp) in 1.841 CPU sec, 1.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290921, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (250.84, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696722 sequences (100000259 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290091, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.94, 118.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697410 reads in 118.181 CPU sec, 3.717 real sec
[M::mem_process_seqs] Processed 696640 reads in 77.588 CPU sec, 2.444 real sec
[M::process] read 696826 sequences (100000000 bp) in 1.711 CPU sec, 1.769 real sec...
sorting block 19, #reads = 1093248 mem = 536885814 peak = 978669568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290122, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.28, 119.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696894 sequences (100000024 bp) in 2.039 CPU sec, 2.122 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290306, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.16, 118.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696722 reads in 118.597 CPU sec, 3.778 real sec
[M::mem_process_seqs] Processed 696826 reads in 78.166 CPU sec, 2.473 real sec
sorting block 20, #reads = 1091867 mem = 536877762 peak = 985128960
[M::process] read 697420 sequences (100000155 bp) in 1.953 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290511, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.89, 120.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701786 sequences (100000186 bp) in 2.164 CPU sec, 2.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290551, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 182, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (197.10, 74.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.15, 121.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696894 reads in 120.626 CPU sec, 3.852 real sec
[M::mem_process_seqs] Processed 697420 reads in 79.775 CPU sec, 2.530 real sec
sorting block 21, #reads = 1091603 mem = 536877040 peak = 992956416
[M::process] read 700280 sequences (100000266 bp) in 1.858 CPU sec, 1.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292084, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.15, 114.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1091539 mem = 536879600 peak = 992956416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291430, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 196, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 265)
[M::mem_pestat] mean and std.dev: (183.12, 21.37)
[M::mem_pestat] low and high boundaries for proper pairs: (98, 292)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.81, 115.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699758 sequences (100000058 bp) in 2.295 CPU sec, 2.469 real sec...
[M::mem_process_seqs] Processed 701786 reads in 118.859 CPU sec, 3.795 real sec
[M::mem_process_seqs] Processed 700280 reads in 78.208 CPU sec, 2.479 real sec
[M::process] read 698830 sequences (100000295 bp) in 2.106 CPU sec, 2.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291499, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.16, 117.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 336, 2270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6264)
[M::mem_pestat] mean and std.dev: (1504.77, 1821.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8789)
[M::mem_pestat] skip orientation RR
sorting block 23, #reads = 1095789 mem = 536879804 peak = 992956416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291071, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.36, 118.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699758 reads in 117.859 CPU sec, 3.768 real sec
[M::process] read 698572 sequences (100000150 bp) in 2.254 CPU sec, 2.400 real sec...
[M::mem_process_seqs] Processed 698830 reads in 77.386 CPU sec, 2.468 real sec
sorting block 24, #reads = 1095056 mem = 536877772 peak = 992956416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291363, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.29, 118.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698572 reads in 65.968 CPU sec, 2.197 real sec
[M::process] read 698508 sequences (100000205 bp) in 2.372 CPU sec, 2.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291526, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.46, 120.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1093746 mem = 536879510 peak = 992956416
[M::mem_process_seqs] Processed 698508 reads in 65.890 CPU sec, 2.174 real sec
[M::process] read 697938 sequences (100000168 bp) in 2.187 CPU sec, 2.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290989, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.74, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697938 reads in 66.677 CPU sec, 2.190 real sec
sorting block 26, #reads = 1094304 mem = 536880839 peak = 992956416
[M::process] read 697082 sequences (100000027 bp) in 2.459 CPU sec, 2.835 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290489, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.10, 120.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 388, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (272.50, 133.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1396)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697082 reads in 66.190 CPU sec, 2.151 real sec
[M::process] read 696824 sequences (100000072 bp) in 2.519 CPU sec, 2.869 real sec...
sorting block 27, #reads = 1093422 mem = 536878016 peak = 992956416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290546, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (254.39, 120.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696460 sequences (100000290 bp) in 1.926 CPU sec, 2.104 real sec...
[M::mem_process_seqs] Processed 696824 reads in 66.668 CPU sec, 2.159 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290647, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.60, 120.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696460 reads in 65.959 CPU sec, 2.144 real sec
[M::process] read 696582 sequences (100000060 bp) in 2.421 CPU sec, 2.651 real sec...
sorting block 28, #reads = 1093514 mem = 536877922 peak = 992956416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290226, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.55, 118.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696642 sequences (100000095 bp) in 1.691 CPU sec, 1.764 real sec...
[M::mem_process_seqs] Processed 696582 reads in 65.816 CPU sec, 2.121 real sec
sorting block 29, #reads = 1093257 mem = 536877861 peak = 1003249664
[M::process] read 696772 sequences (100000127 bp) in 1.932 CPU sec, 2.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290320, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.92, 118.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696774 sequences (100000175 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290298, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.45, 118.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696642 reads in 116.882 CPU sec, 3.707 real sec
[M::mem_process_seqs] Processed 696772 reads in 76.700 CPU sec, 2.421 real sec
[M::process] read 696760 sequences (100000099 bp) in 2.029 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 290345, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 243, 713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1783)
[M::mem_pestat] mean and std.dev: (386.95, 374.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2318)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (250.40, 117.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 30, #reads = 1092370 mem = 536882145 peak = 1003249664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290214, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (250.65, 117.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696770 sequences (100000271 bp) in 1.958 CPU sec, 2.040 real sec...
[M::mem_process_seqs] Processed 696774 reads in 118.199 CPU sec, 3.790 real sec
[M::mem_process_seqs] Processed 696760 reads in 77.986 CPU sec, 2.473 real sec
sorting block 31, #reads = 1091197 mem = 536881737 peak = 1003249664
[M::process] read 696714 sequences (100000040 bp) in 1.748 CPU sec, 1.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290497, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.22, 118.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696458 sequences (100000032 bp) in 1.786 CPU sec, 1.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290751, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.77, 120.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696770 reads in 118.692 CPU sec, 3.770 real sec
[M::mem_process_seqs] Processed 696714 reads in 77.607 CPU sec, 2.454 real sec
sorting block 32, #reads = 1091668 mem = 536878418 peak = 1031000064
[M::process] read 700182 sequences (100000214 bp) in 1.942 CPU sec, 2.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290151, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.40, 121.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291480, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 278, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 451)
[M::mem_pestat] mean and std.dev: (232.91, 47.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 531)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 33, #reads = 1092778 mem = 536881081 peak = 1031000064
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.05, 117.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (281.55, 124.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 700946 sequences (100000091 bp) in 2.349 CPU sec, 2.556 real sec...
[M::mem_process_seqs] Processed 696458 reads in 119.468 CPU sec, 3.836 real sec
[M::mem_process_seqs] Processed 700182 reads in 79.348 CPU sec, 2.524 real sec
[M::process] read 699756 sequences (100000228 bp) in 1.948 CPU sec, 2.049 real sec...
sorting block 34, #reads = 1090505 mem = 536889034 peak = 1031000064
[M::process] read 698856 sequences (100000105 bp) in 1.729 CPU sec, 1.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290916, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 283, 9241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27377)
[M::mem_pestat] mean and std.dev: (2544.25, 3873.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 36445)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.94, 117.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699756 reads in 65.911 CPU sec, 2.111 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291416, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.02, 118.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698456 sequences (100000091 bp) in 2.237 CPU sec, 2.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291732, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.20, 116.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698856 reads in 77.311 CPU sec, 2.507 real sec
sorting block 35, #reads = 1095596 mem = 536879384 peak = 1031000064
[M::mem_process_seqs] Processed 700946 reads in 195.030 CPU sec, 6.298 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291293, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.91, 119.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698456 reads in 65.596 CPU sec, 2.191 real sec
[M::process] read 698000 sequences (100000079 bp) in 2.547 CPU sec, 2.791 real sec...
sorting block 36, #reads = 1095319 mem = 536878837 peak = 1031000064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290566, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.49, 121.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698000 reads in 65.056 CPU sec, 2.170 real sec
[M::process] read 698034 sequences (100000287 bp) in 2.457 CPU sec, 2.730 real sec...
sorting block 37, #reads = 1094938 mem = 536880001 peak = 1031000064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290718, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.68, 122.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698034 reads in 65.905 CPU sec, 2.193 real sec
[M::process] read 697568 sequences (100000229 bp) in 2.194 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290876, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (253.79, 121.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697568 reads in 65.074 CPU sec, 2.137 real sec
sorting block 38, #reads = 1093965 mem = 536880841 peak = 1032552448
[M::process] read 697392 sequences (100000246 bp) in 2.788 CPU sec, 3.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290832, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.10, 121.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697392 reads in 65.833 CPU sec, 2.143 real sec
sorting block 39, #reads = 1095244 mem = 536877589 peak = 1032552448
[M::process] read 696174 sequences (100000121 bp) in 2.597 CPU sec, 2.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290182, 7, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 650, 972)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2584)
[M::mem_pestat] mean and std.dev: (525.57, 389.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3390)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.38, 121.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 162, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (244.08, 165.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696174 reads in 65.665 CPU sec, 2.129 real sec
[M::process] read 696524 sequences (100000001 bp) in 2.145 CPU sec, 2.386 real sec...
sorting block 40, #reads = 1092707 mem = 536880915 peak = 1032552448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290594, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.72, 120.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697360 sequences (100000057 bp) in 1.757 CPU sec, 1.855 real sec...
[M::mem_process_seqs] Processed 696524 reads in 65.519 CPU sec, 2.120 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290394, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (248.97, 116.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697360 reads in 66.031 CPU sec, 2.141 real sec
[M::process] read 697196 sequences (100000025 bp) in 2.210 CPU sec, 2.406 real sec...
sorting block 41, #reads = 1093324 mem = 536878820 peak = 1032552448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290465, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (341, 341, 1335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3323)
[M::mem_pestat] mean and std.dev: (622.18, 455.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4317)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.43, 118.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696754 sequences (100000106 bp) in 1.824 CPU sec, 1.904 real sec...
[M::mem_process_seqs] Processed 697196 reads in 66.099 CPU sec, 2.138 real sec
[M::process] read 696942 sequences (100000130 bp) in 1.735 CPU sec, 1.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290099, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 229, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (193.75, 88.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (250.28, 117.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696764 sequences (100000156 bp) in 1.320 CPU sec, 1.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290224, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (249.85, 116.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696754 reads in 116.812 CPU sec, 3.674 real sec
sorting block 42, #reads = 1092783 mem = 536879594 peak = 1032552448
[M::mem_process_seqs] Processed 696942 reads in 76.679 CPU sec, 2.420 real sec
[M::process] read 696736 sequences (100000122 bp) in 1.628 CPU sec, 1.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290540, 9, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 373, 840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206)
[M::mem_pestat] mean and std.dev: (461.80, 395.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2889)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.30, 118.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 43, #reads = 1091941 mem = 536878606 peak = 1094631424
[M::process] read 695600 sequences (100000282 bp) in 1.888 CPU sec, 2.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290527, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.95, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696764 reads in 117.123 CPU sec, 3.734 real sec
[M::mem_process_seqs] Processed 696736 reads in 76.883 CPU sec, 2.433 real sec
[M::process] read 698778 sequences (100000001 bp) in 1.776 CPU sec, 1.817 real sec...
sorting block 44, #reads = 1091995 mem = 536883578 peak = 1094631424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289951, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 155, 889)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2357)
[M::mem_pestat] mean and std.dev: (632.00, 696.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3418)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (256.56, 122.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (541, 541, 618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (387, 772)
[M::mem_pestat] mean and std.dev: (579.50, 38.50)
[M::mem_pestat] low and high boundaries for proper pairs: (310, 849)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 707044 sequences (100000144 bp) in 2.153 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291067, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.06, 118.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695600 reads in 118.612 CPU sec, 3.775 real sec
[M::mem_process_seqs] Processed 698778 reads in 79.218 CPU sec, 2.509 real sec
sorting block 45, #reads = 1093277 mem = 536878444 peak = 1094631424
[M::process] read 705224 sequences (100000102 bp) in 2.259 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293769, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.19, 108.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (329, 570, 2055)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5507)
[M::mem_pestat] mean and std.dev: (908.27, 785.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7233)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293356, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.70, 112.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707044 reads in 126.720 CPU sec, 4.075 real sec
[M::process] read 704126 sequences (100000030 bp) in 2.346 CPU sec, 2.560 real sec...
[M::mem_process_seqs] Processed 705224 reads in 83.376 CPU sec, 2.654 real sec
sorting block 46, #reads = 1092900 mem = 536880640 peak = 1094631424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 293264, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (237.53, 115.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (342, 844, 903)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2025)
[M::mem_pestat] mean and std.dev: (648.10, 287.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2586)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 702212 sequences (100000087 bp) in 2.107 CPU sec, 2.293 real sec...
[M::mem_process_seqs] Processed 704126 reads in 69.673 CPU sec, 2.294 real sec
sorting block 47, #reads = 1095773 mem = 536878245 peak = 1099100160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292454, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.70, 116.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700998 sequences (100000072 bp) in 2.238 CPU sec, 2.419 real sec...
[M::mem_process_seqs] Processed 702212 reads in 69.158 CPU sec, 2.283 real sec
sorting block 48, #reads = 1095528 mem = 536877155 peak = 1099100160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291647, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.99, 120.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 248, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (248, 248)
[M::mem_pestat] mean and std.dev: (248.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (248, 248)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700998 reads in 67.693 CPU sec, 2.214 real sec
[M::process] read 701042 sequences (100000167 bp) in 2.379 CPU sec, 2.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292098, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (247.03, 121.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1094193 mem = 536878564 peak = 1099100160
[M::process] read 700588 sequences (100000033 bp) in 1.939 CPU sec, 2.171 real sec...
[M::mem_process_seqs] Processed 701042 reads in 68.207 CPU sec, 2.233 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291573, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.06, 120.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700588 reads in 73.401 CPU sec, 2.384 real sec
[M::process] read 700540 sequences (100000016 bp) in 2.636 CPU sec, 2.849 real sec...
sorting block 50, #reads = 1093636 mem = 536879746 peak = 1099100160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292122, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.49, 120.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700540 reads in 70.457 CPU sec, 2.287 real sec
[M::process] read 700532 sequences (100000143 bp) in 2.182 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292070, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.31, 120.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700532 reads in 69.189 CPU sec, 2.246 real sec
sorting block 51, #reads = 1090622 mem = 536878293 peak = 1099100160
[M::process] read 699936 sequences (100000197 bp) in 2.722 CPU sec, 3.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291408, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.95, 120.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699936 reads in 69.906 CPU sec, 2.267 real sec
[M::process] read 698646 sequences (100000216 bp) in 2.217 CPU sec, 2.406 real sec...
sorting block 52, #reads = 1092629 mem = 536881928 peak = 1099100160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290941, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (251.21, 121.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698646 reads in 69.988 CPU sec, 2.270 real sec
[M::process] read 698044 sequences (100000167 bp) in 2.450 CPU sec, 2.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291039, 14, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (252.02, 122.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (247, 249, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (301.64, 142.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698044 reads in 70.792 CPU sec, 2.292 real sec
sorting block 53, #reads = 1091116 mem = 536881290 peak = 1099100160
[M::process] read 698282 sequences (100000024 bp) in 2.376 CPU sec, 2.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290943, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.30, 120.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 176, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (186.56, 92.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RR
[M::process] read 697458 sequences (100000197 bp) in 1.834 CPU sec, 1.935 real sec...
[M::mem_process_seqs] Processed 698282 reads in 71.136 CPU sec, 2.295 real sec
sorting block 54, #reads = 1090993 mem = 536879524 peak = 1099100160
[M::process] read 697166 sequences (100000082 bp) in 1.976 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290386, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.84, 121.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697286 sequences (100000087 bp) in 1.420 CPU sec, 1.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290425, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.83, 123.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697458 reads in 124.758 CPU sec, 3.922 real sec
[M::mem_process_seqs] Processed 697166 reads in 81.596 CPU sec, 2.571 real sec
[M::process] read 707368 sequences (100000182 bp) in 1.744 CPU sec, 1.789 real sec...
sorting block 55, #reads = 1090564 mem = 536879343 peak = 1099100160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290346, 10, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.98, 123.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 223, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311)
[M::mem_pestat] mean and std.dev: (325.00, 334.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1743)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 316, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1710)
[M::mem_pestat] mean and std.dev: (408.27, 255.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2204)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 706626 sequences (100000010 bp) in 2.559 CPU sec, 2.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 294137, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.16, 108.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697286 reads in 132.116 CPU sec, 4.209 real sec
[M::mem_process_seqs] Processed 707368 reads in 90.080 CPU sec, 2.854 real sec
sorting block 56, #reads = 1090692 mem = 536877667 peak = 1099100160
[M::process] read 705046 sequences (100000166 bp) in 2.184 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293391, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.07, 109.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704728 sequences (100000128 bp) in 2.320 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292898, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 320, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461)
[M::mem_pestat] mean and std.dev: (455.40, 372.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1945)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.44, 110.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 706626 reads in 136.020 CPU sec, 4.331 real sec
[M::mem_process_seqs] Processed 705046 reads in 88.496 CPU sec, 2.801 real sec
sorting block 57, #reads = 1091371 mem = 536878092 peak = 1099100160
[M::process] read 703948 sequences (100000199 bp) in 1.950 CPU sec, 2.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292966, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (234.69, 111.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1090712 mem = 536880364 peak = 1126715392
[M::process] read 702708 sequences (100000262 bp) in 1.832 CPU sec, 1.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292929, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (236.53, 112.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704728 reads in 136.916 CPU sec, 4.352 real sec
[M::process] read 702624 sequences (100000080 bp) in 1.564 CPU sec, 1.577 real sec...
[M::mem_process_seqs] Processed 703948 reads in 88.818 CPU sec, 2.805 real sec
sorting block 59, #reads = 1089126 mem = 536880164 peak = 1126715392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292391, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 140, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (187.08, 87.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (240.11, 115.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 218, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (225.90, 119.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 701516 sequences (100000244 bp) in 2.071 CPU sec, 2.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292961, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.46, 115.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702708 reads in 130.639 CPU sec, 4.159 real sec
[M::mem_process_seqs] Processed 702624 reads in 86.848 CPU sec, 2.742 real sec
[M::process] read 701688 sequences (100000291 bp) in 1.745 CPU sec, 1.773 real sec...
sorting block 60, #reads = 1091002 mem = 536884657 peak = 1126715392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292283, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.92, 117.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700378 sequences (100000009 bp) in 2.077 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292357, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.10, 116.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701516 reads in 128.705 CPU sec, 4.092 real sec
[M::process] read 700930 sequences (100000088 bp) in 1.515 CPU sec, 1.518 real sec...
[M::mem_process_seqs] Processed 701688 reads in 85.312 CPU sec, 2.697 real sec
sorting block 61, #reads = 1090344 mem = 536880905 peak = 1128185856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292092, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.49, 119.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699134 sequences (100000173 bp) in 2.292 CPU sec, 2.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291968, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 321, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (87, 477)
[M::mem_pestat] mean and std.dev: (289.23, 38.98)
[M::mem_pestat] low and high boundaries for proper pairs: (9, 555)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.86, 117.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (348, 897, 1126)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2682)
[M::mem_pestat] mean and std.dev: (798.64, 374.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3460)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700378 reads in 125.708 CPU sec, 4.018 real sec
sorting block 62, #reads = 1090984 mem = 536881480 peak = 1128185856
[M::mem_process_seqs] Processed 700930 reads in 82.691 CPU sec, 2.619 real sec
[M::process] read 698786 sequences (100000200 bp) in 1.873 CPU sec, 1.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290962, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (251.06, 123.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 974, 2559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7173)
[M::mem_pestat] mean and std.dev: (1137.58, 1050.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9480)
[M::mem_pestat] skip orientation RR
sorting block 63, #reads = 1091319 mem = 536879289 peak = 1141260288
[M::process] read 698510 sequences (100000268 bp) in 2.314 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290819, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.99, 122.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 244, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (259.25, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699134 reads in 125.585 CPU sec, 4.001 real sec
[M::mem_process_seqs] Processed 698786 reads in 83.342 CPU sec, 2.642 real sec
[M::process] read 697750 sequences (100000132 bp) in 2.301 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290984, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (252.02, 123.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 420, 1114)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2968)
[M::mem_pestat] mean and std.dev: (455.45, 385.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3895)
[M::mem_pestat] skip orientation RR
sorting block 64, #reads = 1090960 mem = 536880884 peak = 1141260288
[M::process] read 699252 sequences (100000226 bp) in 1.930 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290560, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (253.41, 123.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698510 reads in 128.054 CPU sec, 4.100 real sec
[M::mem_process_seqs] Processed 697750 reads in 85.604 CPU sec, 2.709 real sec
sorting block 65, #reads = 1090400 mem = 536880311 peak = 1141260288
[M::process] read 705560 sequences (100000201 bp) in 2.308 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290791, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.82, 121.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293165, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (232.23, 110.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703814 sequences (100000039 bp) in 2.351 CPU sec, 2.530 real sec...
[M::mem_process_seqs] Processed 699252 reads in 138.859 CPU sec, 4.442 real sec
sorting block 66, #reads = 1089270 mem = 536880370 peak = 1141260288
[M::mem_process_seqs] Processed 705560 reads in 88.683 CPU sec, 2.813 real sec
[M::process] read 702692 sequences (100000085 bp) in 1.982 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293096, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (237.01, 112.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1087175 mem = 536880401 peak = 1143689216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292765, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (357, 567, 1180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2826)
[M::mem_pestat] mean and std.dev: (692.90, 421.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3649)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.53, 114.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701532 sequences (100000162 bp) in 2.256 CPU sec, 2.420 real sec...
[M::mem_process_seqs] Processed 703814 reads in 125.684 CPU sec, 4.008 real sec
[M::mem_process_seqs] Processed 702692 reads in 81.679 CPU sec, 2.590 real sec
[M::process] read 701706 sequences (100000109 bp) in 2.299 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292091, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.67, 117.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1094885 mem = 536886631 peak = 1143689216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292332, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.23, 117.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701532 reads in 121.827 CPU sec, 3.911 real sec
[M::process] read 701008 sequences (100000213 bp) in 2.218 CPU sec, 2.331 real sec...
[M::mem_process_seqs] Processed 701706 reads in 80.183 CPU sec, 2.556 real sec
sorting block 69, #reads = 1094619 mem = 536876939 peak = 1143689216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292253, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.20, 118.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701008 reads in 67.458 CPU sec, 2.243 real sec
[M::process] read 701214 sequences (100000283 bp) in 2.750 CPU sec, 3.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292176, 11, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.06, 118.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 581, 2022)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5814)
[M::mem_pestat] mean and std.dev: (689.90, 754.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7710)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 70, #reads = 1095068 mem = 536878216 peak = 1143689216
[M::mem_process_seqs] Processed 701214 reads in 70.402 CPU sec, 2.332 real sec
[M::process] read 700448 sequences (100000162 bp) in 2.817 CPU sec, 3.063 real sec...
sorting block 71, #reads = 1094643 mem = 536881223 peak = 1143689216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291922, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (257, 323, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118)
[M::mem_pestat] mean and std.dev: (320.70, 127.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1405)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.15, 118.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699390 sequences (100000118 bp) in 2.088 CPU sec, 2.199 real sec...
[M::mem_process_seqs] Processed 700448 reads in 70.420 CPU sec, 2.287 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291588, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 421, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1438)
[M::mem_pestat] mean and std.dev: (367.10, 233.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1863)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.81, 119.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699390 reads in 69.065 CPU sec, 2.244 real sec
[M::process] read 699142 sequences (100000236 bp) in 2.553 CPU sec, 2.784 real sec...
sorting block 72, #reads = 1092663 mem = 536880100 peak = 1143689216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 291346, 8, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (310, 419, 1944)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5212)
[M::mem_pestat] mean and std.dev: (878.88, 833.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6846)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.02, 121.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 342, 1117)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3015)
[M::mem_pestat] mean and std.dev: (513.60, 402.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3964)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698926 sequences (100000229 bp) in 1.855 CPU sec, 1.944 real sec...
[M::mem_process_seqs] Processed 699142 reads in 68.640 CPU sec, 2.214 real sec
[M::process] read 698586 sequences (100000301 bp) in 1.868 CPU sec, 1.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291005, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.68, 121.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698264 sequences (100000057 bp) in 1.380 CPU sec, 1.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291275, 6, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 153, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 361)
[M::mem_pestat] mean and std.dev: (177.20, 39.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 431)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.57, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698926 reads in 121.704 CPU sec, 3.826 real sec
sorting block 73, #reads = 1092732 mem = 536882631 peak = 1143689216
[M::mem_process_seqs] Processed 698586 reads in 79.682 CPU sec, 2.510 real sec
[M::process] read 698038 sequences (100000047 bp) in 2.068 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291146, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.22, 121.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1092037 mem = 536879507 peak = 1143689216
[M::process] read 698092 sequences (100000096 bp) in 2.062 CPU sec, 2.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291005, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.65, 120.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698264 reads in 124.446 CPU sec, 3.981 real sec
[M::mem_process_seqs] Processed 698038 reads in 82.180 CPU sec, 2.602 real sec
sorting block 75, #reads = 1091448 mem = 536883299 peak = 1143689216
[M::process] read 698476 sequences (100000174 bp) in 2.514 CPU sec, 2.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290471, 8, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 273, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (236.10, 112.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.14, 120.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290690, 13, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.61, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 296, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (384.38, 251.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1460)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698092 reads in 127.634 CPU sec, 4.082 real sec
[M::process] read 699154 sequences (100000096 bp) in 2.164 CPU sec, 2.294 real sec...
[M::mem_process_seqs] Processed 698476 reads in 84.876 CPU sec, 2.695 real sec
sorting block 76, #reads = 1089289 mem = 536877884 peak = 1143689216
[M::process] read 709600 sequences (100000037 bp) in 2.189 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290975, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.51, 119.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 316, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 555)
[M::mem_pestat] mean and std.dev: (285.33, 69.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] skip orientation RR
[M::process] read 706248 sequences (100000250 bp) in 2.038 CPU sec, 2.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 294473, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 200, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (222.60, 104.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 206, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (203.78, 107.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699154 reads in 142.989 CPU sec, 4.553 real sec
[M::mem_process_seqs] Processed 709600 reads in 93.244 CPU sec, 2.950 real sec
sorting block 77, #reads = 1087675 mem = 536877442 peak = 1143689216
[M::process] read 704202 sequences (100000072 bp) in 2.241 CPU sec, 2.400 real sec...
sorting block 78, #reads = 1086241 mem = 536877544 peak = 1143689216
[M::process] read 703454 sequences (100000153 bp) in 2.000 CPU sec, 2.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292991, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.54, 111.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704202 reads in 75.614 CPU sec, 2.384 real sec
[M::process] read 703012 sequences (100000293 bp) in 1.752 CPU sec, 1.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293451, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.06, 109.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 263, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (251.58, 131.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292559, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.32, 114.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706248 reads in 214.306 CPU sec, 6.813 real sec
[M::process] read 701644 sequences (100000212 bp) in 1.528 CPU sec, 1.546 real sec...
[M::mem_process_seqs] Processed 703454 reads in 107.607 CPU sec, 3.417 real sec
sorting block 79, #reads = 1091016 mem = 536878371 peak = 1143689216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292460, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.27, 115.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1088468 mem = 536880820 peak = 1156202496
[M::process] read 701366 sequences (100000109 bp) in 2.364 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291841, 10, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (243.14, 117.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 130, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (176.56, 103.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 265, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (243.64, 69.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 703012 reads in 130.708 CPU sec, 4.165 real sec
[M::mem_process_seqs] Processed 701644 reads in 85.283 CPU sec, 2.695 real sec
[M::process] read 700196 sequences (100000031 bp) in 2.051 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291967, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.52, 116.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 332, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014)
[M::mem_pestat] mean and std.dev: (416.60, 412.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2626)
[M::mem_pestat] skip orientation RR
sorting block 81, #reads = 1090704 mem = 536876626 peak = 1156202496
[M::process] read 698908 sequences (100000296 bp) in 2.241 CPU sec, 2.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291144, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.58, 118.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701366 reads in 131.760 CPU sec, 4.212 real sec
[M::mem_process_seqs] Processed 700196 reads in 86.808 CPU sec, 2.747 real sec
[M::process] read 699614 sequences (100000034 bp) in 1.941 CPU sec, 2.019 real sec...
sorting block 82, #reads = 1090759 mem = 536878681 peak = 1156202496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291520, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.31, 119.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698762 sequences (100000242 bp) in 2.351 CPU sec, 2.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291414, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.39, 117.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698908 reads in 129.385 CPU sec, 4.129 real sec
[M::mem_process_seqs] Processed 699614 reads in 85.381 CPU sec, 2.705 real sec
sorting block 83, #reads = 1089503 mem = 536881428 peak = 1156202496
[M::process] read 698830 sequences (100000092 bp) in 2.116 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291125, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 396, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (291.00, 155.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.84, 118.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698528 sequences (100000244 bp) in 1.875 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290930, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.20, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (335, 5337, 6800)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19730)
[M::mem_pestat] mean and std.dev: (3444.20, 3093.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 26195)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698762 reads in 126.369 CPU sec, 4.021 real sec
sorting block 84, #reads = 1089768 mem = 536878903 peak = 1157652480
[M::mem_process_seqs] Processed 698830 reads in 82.218 CPU sec, 2.598 real sec
[M::process] read 698584 sequences (100000161 bp) in 2.289 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290644, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.81, 119.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 85, #reads = 1090777 mem = 536878964 peak = 1157652480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290521, 3, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.94, 120.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 301, 951)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2561)
[M::mem_pestat] mean and std.dev: (455.00, 355.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3366)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698528 reads in 125.211 CPU sec, 4.025 real sec
[M::process] read 697212 sequences (100000044 bp) in 2.349 CPU sec, 2.566 real sec...
[M::mem_process_seqs] Processed 698584 reads in 83.011 CPU sec, 2.644 real sec
sorting block 86, #reads = 1090683 mem = 536879814 peak = 1157652480
[M::process] read 697844 sequences (100000243 bp) in 2.054 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289899, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (253.11, 122.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 46284 sequences (6612671 bp) in 0.383 CPU sec, 0.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290807, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.49, 121.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697212 reads in 127.796 CPU sec, 4.125 real sec
[M::mem_process_seqs] Processed 697844 reads in 82.381 CPU sec, 2.674 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 19228, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.40, 118.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 46284 reads in 5.479 CPU sec, 0.192 real sec
sorting block 87, #reads = 1089665 mem = 536880899 peak = 1157652480
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96560170
overall: 69825294336 mem 10439.927 user 364.370 sys 1207.255 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1207.299 sec; CPU: 10804.305 sec
sorting block 88, #reads = 923500 mem = 455643449 peak = 1207197696
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5260201446
output reads: 97052347
bam_mem_sort: 89 calls 32.843 user 0.293 sys 33.283 real
bam_write: 89 calls 101.259 user 1.810 sys 103.277 real
execute: 1 calls 22.868 user 0.963 sys 1236.962 real
merge_files: 1 calls 22.835 user 0.948 sys 28.162 real
parse_chunk: 1422 calls 46.875 user 15.147 sys 62.962 real
read_chunk: 3841 calls 35.830 user 26.664 sys 335.139 real
sort_block: 1 calls 25.585 user 26.327 sys 321.091 real
write_chunk: 2417 calls 22.447 user 0.708 sys 27.280 real
overall: 3030945792 mem 1080.386 user 50.623 sys 1236.985 real
ls: cannot access '*.cram': No such file or directory