.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1136_27F-T1-TDNA-1_A23MHWWLT4_1;L002;0004)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/72/aee21d6fc61e3353d1ceaaa8ec1d8b/.command.log
Size: 162.5 KB
Task: DAQ:SENTIEON_BWAMEM (1136_27F-T1-TDNA-1_A23MHWWLT4_1;L002;0004)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/72/aee21d6fc61e3353d1ceaaa8ec1d8b/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/00/1f27724199272febb63ec65e4f67d7/1136_27F-T1-TDNA-1_A23MHWWLT4_1/0004.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/72/aee21d6fc61e3353d1ceaaa8ec1d8b/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/00/1f27724199272febb63ec65e4f67d7/1136_27F-T1-TDNA-1_A23MHWWLT4_1/0004.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_27F-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1996.460 CPU sec, 78.018 real sec
[M::process] read 701150 sequences (100000287 bp) in 1.246 CPU sec, 1.249 real sec...
[M::process] read 699004 sequences (100000219 bp) in 1.586 CPU sec, 1.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 292539, 26, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 277, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (291.61, 187.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1375)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 235, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.69, 128.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (95, 550, 1851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5363)
[M::mem_pestat] mean and std.dev: (833.71, 1017.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7119)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 279, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (292.29, 164.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698304 sequences (100000290 bp) in 1.468 CPU sec, 1.475 real sec...
[M::process] read 697462 sequences (100000206 bp) in 1.496 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 291849, 18, 88)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 352, 680)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1706)
[M::mem_pestat] mean and std.dev: (409.99, 338.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2219)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (265.48, 132.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 720, 1974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5456)
[M::mem_pestat] mean and std.dev: (893.25, 890.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7197)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 315, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455)
[M::mem_pestat] mean and std.dev: (377.25, 263.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1882)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701150 reads in 169.766 CPU sec, 5.398 real sec
[M::mem_process_seqs] Processed 699004 reads in 106.979 CPU sec, 3.430 real sec
sorting block 0, #reads = 1090725 mem = 536882357 peak = 591396864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 291089, 16, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 273, 811)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2083)
[M::mem_pestat] mean and std.dev: (459.31, 429.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2719)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::process] read 696812 sequences (100000228 bp) in 2.165 CPU sec, 2.257 real sec...
[M::mem_pestat] mean and std.dev: (268.42, 134.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (405, 1252, 2145)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5625)
[M::mem_pestat] mean and std.dev: (1206.69, 847.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7365)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 323, 660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1598)
[M::mem_pestat] mean and std.dev: (356.41, 245.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2067)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696640 sequences (100000213 bp) in 1.487 CPU sec, 1.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 291012, 26, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 310, 572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362)
[M::mem_pestat] mean and std.dev: (357.95, 264.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1757)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (271.71, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 691, 2055)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5689)
[M::mem_pestat] mean and std.dev: (931.64, 794.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7506)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 317, 778)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976)
[M::mem_pestat] mean and std.dev: (433.57, 435.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2575)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698304 reads in 166.906 CPU sec, 5.352 real sec
[M::mem_process_seqs] Processed 697462 reads in 106.941 CPU sec, 3.444 real sec
sorting block 1, #reads = 1089508 mem = 536878644 peak = 718798848
[M::process] read 696756 sequences (100000053 bp) in 2.050 CPU sec, 2.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 290431, 32, 68)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 222, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (252.06, 164.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (273.10, 136.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (472, 948, 3073)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8275)
[M::mem_pestat] mean and std.dev: (1652.00, 1923.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10876)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 292, 631)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1555)
[M::mem_pestat] mean and std.dev: (404.58, 303.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2017)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696588 sequences (100000075 bp) in 1.716 CPU sec, 1.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 290916, 11, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 408, 775)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1863)
[M::mem_pestat] mean and std.dev: (464.21, 347.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2407)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (272.80, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (299, 710, 1087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2663)
[M::mem_pestat] mean and std.dev: (716.80, 704.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3535)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 375, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839)
[M::mem_pestat] mean and std.dev: (496.70, 451.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2390)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290275, 30, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 315, 568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300)
[M::mem_pestat] mean and std.dev: (353.62, 221.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (271.32, 135.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (322, 368, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234)
[M::mem_pestat] mean and std.dev: (415.44, 259.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1538)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 427, 1072)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2808)
[M::mem_pestat] mean and std.dev: (654.64, 626.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3676)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696812 reads in 241.861 CPU sec, 7.839 real sec
[M::mem_process_seqs] Processed 696640 reads in 178.147 CPU sec, 5.782 real sec
sorting block 2, #reads = 1088750 mem = 536881705 peak = 847360000
[M::mem_process_seqs] Processed 696756 reads in 194.616 CPU sec, 6.343 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 290187, 25, 81)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 235, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (286.03, 195.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (271.75, 135.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 354, 1288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3568)
[M::mem_pestat] mean and std.dev: (429.33, 453.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4708)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 229, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213)
[M::mem_pestat] mean and std.dev: (316.18, 258.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1562)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695530 sequences (100000235 bp) in 2.429 CPU sec, 2.908 real sec...
[M::mem_process_seqs] Processed 696588 reads in 97.137 CPU sec, 3.444 real sec
sorting block 3, #reads = 1087369 mem = 536876683 peak = 847360000
[M::process] read 694790 sequences (100000002 bp) in 2.150 CPU sec, 2.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 290227, 27, 66)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 285, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (314.86, 205.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1451)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 249, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (273.70, 135.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 852, 2145)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6015)
[M::mem_pestat] mean and std.dev: (1457.41, 1493.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7950)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 290, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (324.72, 198.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 4, #reads = 1088044 mem = 536878586 peak = 847360000
[M::process] read 694274 sequences (100000157 bp) in 2.149 CPU sec, 2.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 289797, 19, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 262, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (281.58, 173.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 254, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (276.56, 136.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 656, 7293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21379)
[M::mem_pestat] mean and std.dev: (3015.16, 3393.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 28422)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 306, 594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322)
[M::mem_pestat] mean and std.dev: (316.61, 170.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1686)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695530 reads in 184.824 CPU sec, 6.377 real sec
[M::process] read 693652 sequences (100000135 bp) in 1.849 CPU sec, 1.894 real sec...
[M::mem_process_seqs] Processed 694790 reads in 119.012 CPU sec, 3.959 real sec
sorting block 5, #reads = 1087734 mem = 536881424 peak = 847360000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 289462, 16, 78)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 276, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (319.80, 195.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 254, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (275.93, 134.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (471, 1148, 1578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3792)
[M::mem_pestat] mean and std.dev: (936.47, 597.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4899)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 353, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200)
[M::mem_pestat] mean and std.dev: (380.07, 230.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1524)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698470 sequences (100000180 bp) in 2.377 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 289505, 20, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 264, 697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789)
[M::mem_pestat] mean and std.dev: (450.73, 429.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2335)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 257, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (279.89, 137.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (607, 795, 2951)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7639)
[M::mem_pestat] mean and std.dev: (1562.15, 1587.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9983)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 281, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258)
[M::mem_pestat] mean and std.dev: (364.85, 274.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1612)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694274 reads in 187.361 CPU sec, 6.844 real sec
[M::process] read 699938 sequences (100000062 bp) in 2.082 CPU sec, 2.393 real sec...
[M::mem_process_seqs] Processed 693652 reads in 121.227 CPU sec, 4.605 real sec
sorting block 6, #reads = 1086862 mem = 536878823 peak = 847360000
[M::process] read 698734 sequences (100000159 bp) in 2.182 CPU sec, 2.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 292184, 28, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 298, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122)
[M::mem_pestat] mean and std.dev: (304.12, 175.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1433)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 240, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (265.33, 134.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 513, 1870)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5364)
[M::mem_pestat] mean and std.dev: (765.60, 849.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7111)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 584, 1375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3611)
[M::mem_pestat] mean and std.dev: (739.66, 643.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4729)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697800 sequences (100000218 bp) in 1.911 CPU sec, 2.096 real sec...
[M::mem_process_seqs] Processed 699938 reads in 109.180 CPU sec, 3.992 real sec
sorting block 7, #reads = 1087836 mem = 536876514 peak = 847360000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 291575, 24, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 276, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363)
[M::mem_pestat] mean and std.dev: (357.85, 281.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1754)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (267.92, 133.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 645, 1046)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2734)
[M::mem_pestat] mean and std.dev: (549.55, 511.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3578)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 270, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427)
[M::mem_pestat] mean and std.dev: (338.08, 262.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1838)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 291745, 25, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (252.39, 154.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (268.64, 135.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 554, 967)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2515)
[M::mem_pestat] mean and std.dev: (490.13, 370.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3289)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 273, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125)
[M::mem_pestat] mean and std.dev: (348.35, 234.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1433)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698470 reads in 298.275 CPU sec, 10.829 real sec
[M::process] read 697222 sequences (100000078 bp) in 2.178 CPU sec, 2.243 real sec...
[M::mem_process_seqs] Processed 698734 reads in 138.237 CPU sec, 4.989 real sec
sorting block 8, #reads = 1090189 mem = 536880669 peak = 847360000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 291536, 24, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 306, 687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653)
[M::mem_pestat] mean and std.dev: (413.27, 321.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2136)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 246, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.62, 137.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 436, 1906)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5278)
[M::mem_pestat] mean and std.dev: (955.96, 999.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6964)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 340, 854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144)
[M::mem_pestat] mean and std.dev: (492.42, 469.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2789)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696516 sequences (100000212 bp) in 2.761 CPU sec, 3.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 291306, 44, 86)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 242, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (286.40, 141.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (273.37, 137.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (889, 1217, 2557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5893)
[M::mem_pestat] mean and std.dev: (1306.44, 832.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7561)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 296, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (323.55, 207.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697800 reads in 180.972 CPU sec, 6.533 real sec
[M::mem_process_seqs] Processed 697222 reads in 116.263 CPU sec, 4.196 real sec
[M::process] read 696848 sequences (100000066 bp) in 1.884 CPU sec, 2.115 real sec...
sorting block 9, #reads = 1091509 mem = 536877324 peak = 847360000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 290109, 22, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 259, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1409)
[M::mem_pestat] mean and std.dev: (340.31, 271.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1830)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (274.92, 138.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 342, 1400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3792)
[M::mem_pestat] mean and std.dev: (756.05, 720.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4988)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 431, 848)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2086)
[M::mem_pestat] mean and std.dev: (480.83, 368.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2705)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696896 sequences (100000228 bp) in 2.355 CPU sec, 2.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 290815, 34, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 215, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102)
[M::mem_pestat] mean and std.dev: (258.64, 206.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1421)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (271.87, 136.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (322, 634, 1660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4336)
[M::mem_pestat] mean and std.dev: (782.30, 704.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5674)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 243, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108)
[M::mem_pestat] mean and std.dev: (309.61, 220.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1421)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696516 reads in 182.516 CPU sec, 6.258 real sec
sorting block 10, #reads = 1089691 mem = 536880155 peak = 864210944
[M::mem_process_seqs] Processed 696848 reads in 117.348 CPU sec, 3.981 real sec
[M::process] read 696324 sequences (100000266 bp) in 1.847 CPU sec, 1.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 290862, 19, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 249, 803)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2073)
[M::mem_pestat] mean and std.dev: (431.12, 411.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2708)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (271.43, 136.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (501, 880, 3279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8835)
[M::mem_pestat] mean and std.dev: (1910.95, 2197.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11613)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 336, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (396.32, 255.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1462)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 11, #reads = 1088731 mem = 536878437 peak = 903147520
[M::process] read 695846 sequences (100000262 bp) in 2.651 CPU sec, 2.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 290626, 19, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 302, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034)
[M::mem_pestat] mean and std.dev: (455.81, 475.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2664)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (272.05, 135.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 476, 1628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4472)
[M::mem_pestat] mean and std.dev: (543.38, 599.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5894)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 363, 568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254)
[M::mem_pestat] mean and std.dev: (398.85, 261.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1597)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696896 reads in 178.308 CPU sec, 5.875 real sec
[M::process] read 695384 sequences (100000183 bp) in 1.899 CPU sec, 1.985 real sec...
[M::mem_process_seqs] Processed 696324 reads in 115.679 CPU sec, 3.761 real sec
sorting block 12, #reads = 1088561 mem = 536877994 peak = 903147520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290298, 35, 92)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 217, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (212.54, 82.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 688)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (273.44, 135.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (299, 601, 2350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6452)
[M::mem_pestat] mean and std.dev: (1385.17, 1687.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8503)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 438, 749)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1799)
[M::mem_pestat] mean and std.dev: (442.87, 332.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2324)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694820 sequences (100000258 bp) in 2.703 CPU sec, 2.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 289885, 18, 87)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 242, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (275.60, 182.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (275.58, 137.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (268, 1142, 1928)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5248)
[M::mem_pestat] mean and std.dev: (1245.06, 1085.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6908)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012)
[M::mem_pestat] mean and std.dev: (399.05, 322.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2610)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695846 reads in 179.424 CPU sec, 5.848 real sec
[M::process] read 695946 sequences (100000041 bp) in 1.949 CPU sec, 1.967 real sec...
[M::mem_process_seqs] Processed 695384 reads in 117.554 CPU sec, 3.819 real sec
sorting block 13, #reads = 1087862 mem = 536879258 peak = 903147520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 289756, 13, 78)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 312, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134)
[M::mem_pestat] mean and std.dev: (315.31, 176.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1448)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 253, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.25, 136.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (465, 506, 853)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629)
[M::mem_pestat] mean and std.dev: (564.33, 279.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2017)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 290, 786)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1984)
[M::mem_pestat] mean and std.dev: (414.51, 417.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2583)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700974 sequences (100000030 bp) in 2.885 CPU sec, 3.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290425, 29, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 715)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1713)
[M::mem_pestat] mean and std.dev: (403.31, 287.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2212)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (275.32, 137.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (297, 610, 1524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3978)
[M::mem_pestat] mean and std.dev: (967.59, 958.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5205)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 305, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (328.98, 210.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694820 reads in 174.500 CPU sec, 5.743 real sec
[M::mem_process_seqs] Processed 695946 reads in 114.479 CPU sec, 3.745 real sec
sorting block 14, #reads = 1087251 mem = 536878367 peak = 903147520
[M::process] read 699070 sequences (100000175 bp) in 2.025 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 292059, 36, 84)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 348, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1566)
[M::mem_pestat] mean and std.dev: (366.54, 288.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2034)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 236, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (260.59, 130.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 956, 1463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3917)
[M::mem_pestat] mean and std.dev: (1060.17, 950.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5144)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 259, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160)
[M::mem_pestat] mean and std.dev: (324.91, 216.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1492)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 15, #reads = 1086723 mem = 536880660 peak = 903147520
[M::process] read 697876 sequences (100000059 bp) in 2.527 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 291826, 24, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 277, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353)
[M::mem_pestat] mean and std.dev: (353.12, 294.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1760)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (266.54, 133.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (334, 746, 758)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1606)
[M::mem_pestat] mean and std.dev: (580.77, 324.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2030)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 360, 906)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2260)
[M::mem_pestat] mean and std.dev: (526.22, 417.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2937)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700974 reads in 175.132 CPU sec, 5.852 real sec
[M::mem_process_seqs] Processed 699070 reads in 115.238 CPU sec, 3.906 real sec
[M::process] read 697044 sequences (100000079 bp) in 2.161 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 291188, 16, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 278, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433)
[M::mem_pestat] mean and std.dev: (344.45, 269.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1851)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (268.95, 133.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 560, 2987)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8537)
[M::mem_pestat] mean and std.dev: (1067.69, 1225.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11312)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 276, 642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1510)
[M::mem_pestat] mean and std.dev: (408.07, 331.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1944)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 16, #reads = 1090823 mem = 536883357 peak = 903147520
[M::process] read 696366 sequences (100000147 bp) in 2.726 CPU sec, 3.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 291575, 19, 88)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 297, 701)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689)
[M::mem_pestat] mean and std.dev: (413.73, 341.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2183)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (272.19, 135.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (368, 1320, 1829)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4751)
[M::mem_pestat] mean and std.dev: (1122.61, 883.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6212)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 461, 735)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723)
[M::mem_pestat] mean and std.dev: (505.71, 365.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2217)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697876 reads in 173.285 CPU sec, 5.702 real sec
[M::mem_process_seqs] Processed 697044 reads in 114.140 CPU sec, 3.718 real sec
[M::process] read 696236 sequences (100000280 bp) in 2.319 CPU sec, 2.592 real sec...
sorting block 17, #reads = 1088587 mem = 536882979 peak = 903147520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 290432, 22, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 234, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (243.84, 94.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (274.17, 136.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (428, 685, 2314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6086)
[M::mem_pestat] mean and std.dev: (1012.89, 1229.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7972)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 264, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (308.11, 179.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696420 sequences (100000094 bp) in 2.909 CPU sec, 3.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 290711, 20, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 314, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (321.96, 171.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (274.35, 137.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (282, 527, 1349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3483)
[M::mem_pestat] mean and std.dev: (740.45, 618.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4550)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 325, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322)
[M::mem_pestat] mean and std.dev: (393.11, 307.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1705)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696366 reads in 180.776 CPU sec, 5.879 real sec
[M::mem_process_seqs] Processed 696236 reads in 118.011 CPU sec, 3.806 real sec
sorting block 18, #reads = 1090226 mem = 536881282 peak = 903147520
[M::process] read 696202 sequences (100000295 bp) in 2.467 CPU sec, 2.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 290569, 27, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 241, 701)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1775)
[M::mem_pestat] mean and std.dev: (276.41, 208.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2312)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (272.77, 136.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 518, 720)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1680)
[M::mem_pestat] mean and std.dev: (462.67, 334.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2160)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 353, 897)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2291)
[M::mem_pestat] mean and std.dev: (553.27, 487.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2988)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695886 sequences (100000089 bp) in 2.471 CPU sec, 2.692 real sec...
sorting block 19, #reads = 1088466 mem = 536878139 peak = 903147520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 290746, 31, 73)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 269, 695)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741)
[M::mem_pestat] mean and std.dev: (340.66, 280.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2264)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (272.04, 135.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (353, 734, 1477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3725)
[M::mem_pestat] mean and std.dev: (919.47, 812.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4849)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 374, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172)
[M::mem_pestat] mean and std.dev: (349.89, 183.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1487)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696420 reads in 177.781 CPU sec, 5.796 real sec
[M::mem_process_seqs] Processed 696202 reads in 116.160 CPU sec, 3.758 real sec
[M::process] read 695392 sequences (100000024 bp) in 2.220 CPU sec, 2.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 290163, 30, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 422, 810)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2092)
[M::mem_pestat] mean and std.dev: (504.34, 393.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2733)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (274.02, 136.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (377, 610, 1050)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2396)
[M::mem_pestat] mean and std.dev: (740.80, 502.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3069)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 403, 751)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815)
[M::mem_pestat] mean and std.dev: (414.45, 288.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2347)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 20, #reads = 1087811 mem = 536877493 peak = 903147520
[M::process] read 694862 sequences (100000221 bp) in 2.643 CPU sec, 3.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 290265, 24, 95)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 323, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900)
[M::mem_pestat] mean and std.dev: (450.73, 345.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2465)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (274.46, 136.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 1183, 2215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6203)
[M::mem_pestat] mean and std.dev: (1382.70, 1454.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8197)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 303, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1837)
[M::mem_pestat] mean and std.dev: (410.16, 333.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2389)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695886 reads in 178.688 CPU sec, 5.947 real sec
[M::mem_process_seqs] Processed 695392 reads in 115.043 CPU sec, 3.815 real sec
[M::process] read 694210 sequences (100000241 bp) in 2.630 CPU sec, 3.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 290190, 22, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 305, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513)
[M::mem_pestat] mean and std.dev: (414.42, 333.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (277.80, 138.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 871, 1684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4616)
[M::mem_pestat] mean and std.dev: (1028.41, 895.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6082)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 290, 745)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857)
[M::mem_pestat] mean and std.dev: (492.44, 444.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2413)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694862 reads in 98.900 CPU sec, 3.361 real sec
sorting block 21, #reads = 1088936 mem = 536878121 peak = 903147520
[M::process] read 700864 sequences (100000039 bp) in 2.512 CPU sec, 3.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 289629, 26, 84)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 223, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (244.13, 123.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 256, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (279.91, 138.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (303, 680, 3680)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10434)
[M::mem_pestat] mean and std.dev: (1726.42, 2203.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13811)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 304, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425)
[M::mem_pestat] mean and std.dev: (361.43, 251.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1837)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694210 reads in 97.143 CPU sec, 3.371 real sec
sorting block 22, #reads = 1087468 mem = 536878474 peak = 914964480
[M::process] read 700064 sequences (100000052 bp) in 2.627 CPU sec, 3.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 291964, 30, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 297, 1524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4260)
[M::mem_pestat] mean and std.dev: (642.28, 792.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5628)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 236, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (262.02, 132.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 906)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 271, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1801)
[M::mem_pestat] mean and std.dev: (358.04, 317.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2381)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 288, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161)
[M::mem_pestat] mean and std.dev: (338.02, 208.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1485)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698800 sequences (100000057 bp) in 2.354 CPU sec, 2.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 292004, 31, 78)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 286, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (293.25, 173.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 239, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (264.84, 134.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 516, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475)
[M::mem_pestat] mean and std.dev: (444.73, 276.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1891)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 424, 623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345)
[M::mem_pestat] mean and std.dev: (430.45, 251.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1706)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700864 reads in 180.927 CPU sec, 5.941 real sec
[M::mem_process_seqs] Processed 700064 reads in 114.054 CPU sec, 3.733 real sec
sorting block 23, #reads = 1087748 mem = 536878048 peak = 914964480
[M::process] read 697936 sequences (100000198 bp) in 2.751 CPU sec, 3.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 291653, 34, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 343, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1851)
[M::mem_pestat] mean and std.dev: (411.16, 349.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2416)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (268.47, 135.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 476, 607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265)
[M::mem_pestat] mean and std.dev: (375.89, 243.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1594)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 303, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (325.53, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 24, #reads = 1091776 mem = 536877489 peak = 914964480
[M::process] read 697920 sequences (100000187 bp) in 2.392 CPU sec, 2.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 291009, 21, 75)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 298, 1149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3127)
[M::mem_pestat] mean and std.dev: (556.34, 539.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4116)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 245, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (270.89, 136.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (413, 747, 1220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2834)
[M::mem_pestat] mean and std.dev: (688.28, 506.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3641)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 292, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205)
[M::mem_pestat] mean and std.dev: (358.48, 262.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1534)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698800 reads in 178.646 CPU sec, 5.829 real sec
[M::mem_process_seqs] Processed 697936 reads in 114.178 CPU sec, 3.731 real sec
[M::process] read 697078 sequences (100000104 bp) in 2.031 CPU sec, 2.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 290999, 15, 71)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 294, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134)
[M::mem_pestat] mean and std.dev: (286.16, 159.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1452)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (270.05, 136.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 521, 1444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3850)
[M::mem_pestat] mean and std.dev: (820.00, 801.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5053)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 332, 854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192)
[M::mem_pestat] mean and std.dev: (499.82, 471.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2861)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 25, #reads = 1091613 mem = 536883912 peak = 914964480
[M::process] read 696894 sequences (100000214 bp) in 2.497 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 290631, 29, 86)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 288, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1510)
[M::mem_pestat] mean and std.dev: (383.04, 334.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1959)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (273.40, 137.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 560, 1371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3797)
[M::mem_pestat] mean and std.dev: (552.79, 459.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5010)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 342, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (310.28, 129.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697920 reads in 176.501 CPU sec, 5.749 real sec
[M::mem_process_seqs] Processed 697078 reads in 115.082 CPU sec, 3.743 real sec
[M::process] read 696612 sequences (100000104 bp) in 2.430 CPU sec, 2.605 real sec...
sorting block 26, #reads = 1089687 mem = 536885827 peak = 914964480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 290244, 30, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 278, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142)
[M::mem_pestat] mean and std.dev: (300.89, 217.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1471)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (272.36, 136.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (525, 705, 1110)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2280)
[M::mem_pestat] mean and std.dev: (687.54, 481.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2865)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 307, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570)
[M::mem_pestat] mean and std.dev: (414.07, 344.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2027)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696210 sequences (100000210 bp) in 2.661 CPU sec, 3.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 290635, 34, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 304, 795)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985)
[M::mem_pestat] mean and std.dev: (471.14, 374.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2580)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (272.77, 136.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 401, 1112)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2872)
[M::mem_pestat] mean and std.dev: (512.57, 553.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3752)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 368, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368)
[M::mem_pestat] mean and std.dev: (382.97, 236.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1747)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696894 reads in 181.497 CPU sec, 5.959 real sec
[M::mem_process_seqs] Processed 696612 reads in 118.958 CPU sec, 3.892 real sec
sorting block 27, #reads = 1088447 mem = 536880250 peak = 914964480
[M::process] read 695246 sequences (100000083 bp) in 2.200 CPU sec, 2.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 290315, 31, 62)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 297, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559)
[M::mem_pestat] mean and std.dev: (361.27, 268.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2012)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (272.34, 135.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (384, 747, 1419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3489)
[M::mem_pestat] mean and std.dev: (900.14, 709.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4524)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 591)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437)
[M::mem_pestat] mean and std.dev: (342.04, 291.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1860)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 694912 sequences (100000122 bp) in 2.616 CPU sec, 3.157 real sec...
sorting block 28, #reads = 1088053 mem = 536885478 peak = 914964480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 290228, 22, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 323, 809)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2035)
[M::mem_pestat] mean and std.dev: (458.44, 378.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2648)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (274.70, 135.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 285, 1095)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3143)
[M::mem_pestat] mean and std.dev: (587.29, 742.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4167)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 269, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (277.25, 155.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696210 reads in 182.336 CPU sec, 6.015 real sec
[M::process] read 694138 sequences (100000117 bp) in 2.461 CPU sec, 2.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 289905, 23, 83)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 297, 710)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792)
[M::mem_pestat] mean and std.dev: (436.11, 376.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2333)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 252, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.43, 134.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (85, 487, 1267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3631)
[M::mem_pestat] mean and std.dev: (696.18, 794.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4813)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 305, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (291.25, 125.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694912 reads in 98.869 CPU sec, 3.217 real sec
sorting block 29, #reads = 1087083 mem = 536878010 peak = 914964480
[M::process] read 702168 sequences (100000248 bp) in 2.386 CPU sec, 2.796 real sec...
[M::mem_process_seqs] Processed 695246 reads in 278.203 CPU sec, 9.061 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 289978, 26, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 288, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094)
[M::mem_pestat] mean and std.dev: (345.76, 205.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1386)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (279.03, 138.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 429, 1162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420)
[M::mem_pestat] mean and std.dev: (652.52, 789.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4549)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 283, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942)
[M::mem_pestat] mean and std.dev: (419.98, 387.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2533)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 703584 sequences (100000246 bp) in 2.750 CPU sec, 3.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 292863, 15, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 409, 1097)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2897)
[M::mem_pestat] mean and std.dev: (615.38, 600.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 231, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (258.00, 131.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (457, 633, 1839)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4603)
[M::mem_pestat] mean and std.dev: (1103.73, 1022.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5985)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (356.00, 198.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694138 reads in 202.372 CPU sec, 6.558 real sec
sorting block 30, #reads = 1087247 mem = 536877339 peak = 914964480
[M::mem_process_seqs] Processed 702168 reads in 121.572 CPU sec, 3.951 real sec
[M::process] read 701884 sequences (100000125 bp) in 2.306 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 293791, 22, 74)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (296.78, 186.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 226, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (254.60, 130.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 453, 1594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4466)
[M::mem_pestat] mean and std.dev: (894.82, 1100.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5902)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 324, 594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448)
[M::mem_pestat] mean and std.dev: (416.85, 323.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1875)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 31, #reads = 1087908 mem = 536885686 peak = 914964480
[M::process] read 700116 sequences (100000232 bp) in 2.668 CPU sec, 3.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 292648, 19, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 336, 859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2231)
[M::mem_pestat] mean and std.dev: (466.62, 419.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2917)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 232, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (260.40, 133.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (317, 995, 2510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6896)
[M::mem_pestat] mean and std.dev: (1451.44, 1553.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9089)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 387, 1494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4108)
[M::mem_pestat] mean and std.dev: (808.67, 896.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5415)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 703584 reads in 185.162 CPU sec, 6.081 real sec
[M::mem_process_seqs] Processed 701884 reads in 120.663 CPU sec, 3.918 real sec
[M::process] read 699218 sequences (100000047 bp) in 2.408 CPU sec, 2.650 real sec...
sorting block 32, #reads = 1092418 mem = 536880183 peak = 914964480
[M::process] read 698848 sequences (100000141 bp) in 2.713 CPU sec, 3.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 292113, 32, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 348, 603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1415)
[M::mem_pestat] mean and std.dev: (371.91, 288.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1821)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 240, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (268.58, 137.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (327, 444, 1020)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2406)
[M::mem_pestat] mean and std.dev: (715.22, 606.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3140)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 265, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (299.32, 145.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699218 reads in 103.394 CPU sec, 3.327 real sec
[M::process] read 698038 sequences (100000147 bp) in 2.080 CPU sec, 2.255 real sec...
sorting block 33, #reads = 1090970 mem = 536878335 peak = 921219072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 292217, 20, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 198, 591)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1447)
[M::mem_pestat] mean and std.dev: (320.90, 305.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1875)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 237, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (264.91, 135.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (633, 912, 2197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5325)
[M::mem_pestat] mean and std.dev: (966.56, 694.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6889)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 323, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1264)
[M::mem_pestat] mean and std.dev: (357.63, 215.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1625)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 291866, 25, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 273, 804)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2054)
[M::mem_pestat] mean and std.dev: (399.58, 352.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2679)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 242, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (270.65, 138.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 1123, 1920)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5466)
[M::mem_pestat] mean and std.dev: (1079.60, 883.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7239)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 244, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (271.54, 152.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700116 reads in 289.906 CPU sec, 9.424 real sec
[M::mem_process_seqs] Processed 698848 reads in 138.496 CPU sec, 4.500 real sec
[M::process] read 697320 sequences (100000055 bp) in 2.321 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 291359, 37, 79)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 269, 798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2076)
[M::mem_pestat] mean and std.dev: (444.04, 455.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2715)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (271.27, 137.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 299, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622)
[M::mem_pestat] mean and std.dev: (332.63, 268.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2129)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 309, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549)
[M::mem_pestat] mean and std.dev: (373.91, 261.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1986)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 34, #reads = 1089388 mem = 536878796 peak = 921219072
[M::process] read 696870 sequences (100000062 bp) in 2.962 CPU sec, 3.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290723, 21, 80)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 253, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109)
[M::mem_pestat] mean and std.dev: (270.12, 154.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1428)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 245, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (271.92, 137.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (381, 1038, 1804)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4650)
[M::mem_pestat] mean and std.dev: (1279.45, 1141.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6073)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 318, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (304.42, 142.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698038 reads in 189.871 CPU sec, 6.228 real sec
[M::mem_process_seqs] Processed 697320 reads in 121.132 CPU sec, 3.973 real sec
[M::process] read 696554 sequences (100000257 bp) in 2.287 CPU sec, 2.424 real sec...
sorting block 35, #reads = 1087931 mem = 536878809 peak = 921219072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 290891, 26, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 283, 672)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1708)
[M::mem_pestat] mean and std.dev: (392.27, 306.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2226)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (273.25, 137.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (348, 717, 1615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4149)
[M::mem_pestat] mean and std.dev: (1083.04, 911.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5416)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 302, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (333.55, 183.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1378)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696280 sequences (100000075 bp) in 2.222 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 290693, 26, 70)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 240, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (262.91, 166.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (272.20, 135.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 343, 761)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1975)
[M::mem_pestat] mean and std.dev: (407.46, 293.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2582)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 394, 624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458)
[M::mem_pestat] mean and std.dev: (435.97, 305.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1875)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696870 reads in 188.965 CPU sec, 6.177 real sec
[M::process] read 697470 sequences (100000166 bp) in 2.023 CPU sec, 2.274 real sec...
[M::mem_process_seqs] Processed 696554 reads in 121.825 CPU sec, 3.975 real sec
sorting block 36, #reads = 1086837 mem = 536877401 peak = 932331520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 290223, 22, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 300, 798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2054)
[M::mem_pestat] mean and std.dev: (467.32, 412.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2682)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (271.68, 135.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (445, 744, 1679)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4147)
[M::mem_pestat] mean and std.dev: (759.11, 506.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5381)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 294, 607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1403)
[M::mem_pestat] mean and std.dev: (367.52, 259.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1801)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 706536 sequences (100000229 bp) in 2.536 CPU sec, 2.912 real sec...
sorting block 37, #reads = 1085571 mem = 536879218 peak = 976056320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290833, 19, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 384, 908)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2366)
[M::mem_pestat] mean and std.dev: (519.62, 486.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3095)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (270.46, 136.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (335, 377, 1090)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2600)
[M::mem_pestat] mean and std.dev: (504.28, 402.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3355)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 300, 847)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181)
[M::mem_pestat] mean and std.dev: (527.97, 512.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696280 reads in 190.635 CPU sec, 6.264 real sec
[M::mem_process_seqs] Processed 697470 reads in 124.471 CPU sec, 4.066 real sec
[M::process] read 703386 sequences (100000148 bp) in 2.453 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 294851, 20, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 223, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147)
[M::mem_pestat] mean and std.dev: (302.72, 234.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (245.10, 123.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (343, 507, 1004)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2326)
[M::mem_pestat] mean and std.dev: (614.53, 404.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2987)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 347, 777)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1935)
[M::mem_pestat] mean and std.dev: (431.04, 331.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2514)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 38, #reads = 1084836 mem = 536879875 peak = 976056320
[M::process] read 703446 sequences (100000173 bp) in 2.352 CPU sec, 2.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 293758, 21, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157)
[M::mem_pestat] mean and std.dev: (278.41, 185.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 227, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.38, 128.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 812, 1190)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3162)
[M::mem_pestat] mean and std.dev: (734.35, 690.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4148)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 212, 651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1607)
[M::mem_pestat] mean and std.dev: (395.13, 380.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2085)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 706536 reads in 191.898 CPU sec, 6.293 real sec
[M::process] read 701406 sequences (100000169 bp) in 2.033 CPU sec, 2.084 real sec...
sorting block 39, #reads = 1088158 mem = 536882112 peak = 976056320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 293415, 22, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 225, 711)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1843)
[M::mem_pestat] mean and std.dev: (382.55, 394.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2409)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 227, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (254.21, 129.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 502, 1093)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2859)
[M::mem_pestat] mean and std.dev: (604.76, 548.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3742)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 693)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1633)
[M::mem_pestat] mean and std.dev: (472.00, 373.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2103)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700478 sequences (100000204 bp) in 2.326 CPU sec, 2.405 real sec...
[M::mem_process_seqs] Processed 703446 reads in 109.941 CPU sec, 3.639 real sec
[M::mem_process_seqs] Processed 703386 reads in 279.111 CPU sec, 9.162 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 292357, 19, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 218, 650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1588)
[M::mem_pestat] mean and std.dev: (324.48, 252.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2057)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 233, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (260.84, 132.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (479, 765, 1626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3920)
[M::mem_pestat] mean and std.dev: (928.18, 712.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5067)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 410, 1149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3059)
[M::mem_pestat] mean and std.dev: (639.60, 584.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4014)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 40, #reads = 1089376 mem = 536881589 peak = 976056320
[M::process] read 699174 sequences (100000058 bp) in 2.476 CPU sec, 2.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 292602, 22, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 299, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1545)
[M::mem_pestat] mean and std.dev: (388.80, 316.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1998)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 236, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (264.43, 135.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 216, 1129)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3023)
[M::mem_pestat] mean and std.dev: (657.67, 775.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3970)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 278, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257)
[M::mem_pestat] mean and std.dev: (333.98, 254.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1615)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701406 reads in 211.085 CPU sec, 6.906 real sec
[M::mem_process_seqs] Processed 700478 reads in 124.976 CPU sec, 4.126 real sec
[M::process] read 698548 sequences (100000096 bp) in 2.351 CPU sec, 2.656 real sec...
sorting block 41, #reads = 1088158 mem = 536882972 peak = 976056320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 291915, 23, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 326, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144)
[M::mem_pestat] mean and std.dev: (335.72, 193.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1459)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 240, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (267.95, 136.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (264, 550, 1045)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2607)
[M::mem_pestat] mean and std.dev: (624.36, 424.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3388)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 348, 901)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303)
[M::mem_pestat] mean and std.dev: (447.60, 408.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3004)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697432 sequences (100000251 bp) in 2.582 CPU sec, 3.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291299, 46, 65)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 281, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1520)
[M::mem_pestat] mean and std.dev: (341.17, 290.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1973)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 242, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (269.82, 137.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 912, 1601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4327)
[M::mem_pestat] mean and std.dev: (837.71, 680.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5690)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 261, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064)
[M::mem_pestat] mean and std.dev: (327.78, 189.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1356)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699174 reads in 193.921 CPU sec, 6.338 real sec
[M::mem_process_seqs] Processed 698548 reads in 126.128 CPU sec, 4.117 real sec
[M::process] read 697238 sequences (100000116 bp) in 1.974 CPU sec, 2.254 real sec...
sorting block 42, #reads = 1087242 mem = 536886490 peak = 976056320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290973, 30, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 378, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1503)
[M::mem_pestat] mean and std.dev: (378.78, 287.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1952)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (271.22, 137.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 419, 1308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3578)
[M::mem_pestat] mean and std.dev: (687.21, 741.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4713)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 260, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (311.97, 164.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696560 sequences (100000013 bp) in 2.688 CPU sec, 3.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 290881, 21, 76)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 219, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (256.73, 160.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 245, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (272.34, 138.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (67, 354, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038)
[M::mem_pestat] mean and std.dev: (440.43, 399.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2695)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 303, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361)
[M::mem_pestat] mean and std.dev: (410.75, 293.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1751)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697432 reads in 189.182 CPU sec, 6.168 real sec
sorting block 43, #reads = 1086676 mem = 536881680 peak = 976056320
[M::mem_process_seqs] Processed 697238 reads in 123.485 CPU sec, 3.974 real sec
[M::process] read 697274 sequences (100000083 bp) in 2.551 CPU sec, 2.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 291021, 21, 68)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 265, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515)
[M::mem_pestat] mean and std.dev: (357.84, 295.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1962)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 247, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (274.65, 139.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (352, 1014, 1369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3403)
[M::mem_pestat] mean and std.dev: (932.05, 569.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4420)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 317, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150)
[M::mem_pestat] mean and std.dev: (315.53, 175.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1469)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 44, #reads = 1086674 mem = 536883161 peak = 1012183040
[M::process] read 704140 sequences (100000053 bp) in 2.612 CPU sec, 3.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 290264, 38, 68)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (260.14, 137.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 243, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (272.38, 140.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (269, 981, 2295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6347)
[M::mem_pestat] mean and std.dev: (1540.43, 1582.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8373)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 346, 1029)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2623)
[M::mem_pestat] mean and std.dev: (554.64, 543.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3420)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696560 reads in 194.747 CPU sec, 6.475 real sec
[M::mem_process_seqs] Processed 697274 reads in 132.424 CPU sec, 4.390 real sec
[M::process] read 701990 sequences (100000048 bp) in 2.138 CPU sec, 2.293 real sec...
sorting block 45, #reads = 1086645 mem = 536880863 peak = 1012183040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 293605, 19, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 219, 777)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2051)
[M::mem_pestat] mean and std.dev: (378.94, 364.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2688)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 225, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (251.75, 128.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (501, 896, 2411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6231)
[M::mem_pestat] mean and std.dev: (1298.82, 1257.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8141)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 433, 848)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152)
[M::mem_pestat] mean and std.dev: (596.23, 509.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2804)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 701196 sequences (100000141 bp) in 2.390 CPU sec, 2.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 293302, 29, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 277, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (273.63, 175.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1350)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 233, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (258.66, 130.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (322, 657, 1985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5311)
[M::mem_pestat] mean and std.dev: (771.04, 704.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6974)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 300, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1154)
[M::mem_pestat] mean and std.dev: (314.23, 172.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1470)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 704140 reads in 184.333 CPU sec, 5.995 real sec
[M::process] read 699964 sequences (100000283 bp) in 2.149 CPU sec, 2.207 real sec...
[M::mem_process_seqs] Processed 701990 reads in 119.336 CPU sec, 3.864 real sec
sorting block 46, #reads = 1085030 mem = 536876203 peak = 1012183040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 292591, 26, 64)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 259, 1039)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749)
[M::mem_pestat] mean and std.dev: (482.79, 546.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3604)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 234, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (260.78, 132.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 633, 990)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2674)
[M::mem_pestat] mean and std.dev: (463.91, 403.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3516)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 303, 879)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2249)
[M::mem_pestat] mean and std.dev: (478.23, 447.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2934)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 699742 sequences (100000180 bp) in 2.573 CPU sec, 2.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 292234, 16, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 234, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (298.29, 213.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 237, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (265.00, 134.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 537, 1977)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5729)
[M::mem_pestat] mean and std.dev: (1027.81, 1267.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7605)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 304, 680)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1664)
[M::mem_pestat] mean and std.dev: (336.59, 216.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2156)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701196 reads in 183.403 CPU sec, 6.055 real sec
[M::mem_process_seqs] Processed 699964 reads in 119.735 CPU sec, 3.914 real sec
sorting block 47, #reads = 1090821 mem = 536882534 peak = 1012183040
[M::process] read 699168 sequences (100000222 bp) in 2.365 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 292492, 15, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 426, 592)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286)
[M::mem_pestat] mean and std.dev: (397.21, 240.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1633)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 238, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.89, 135.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 333, 1004)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2662)
[M::mem_pestat] mean and std.dev: (540.53, 481.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3491)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 305, 1462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3928)
[M::mem_pestat] mean and std.dev: (738.63, 894.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5161)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 48, #reads = 1089693 mem = 536887529 peak = 1012183040
[M::process] read 697982 sequences (100000251 bp) in 2.304 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 292116, 24, 67)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 266, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (280.50, 182.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 240, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (268.47, 137.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 311, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219)
[M::mem_pestat] mean and std.dev: (235.10, 246.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1614)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 360, 690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626)
[M::mem_pestat] mean and std.dev: (465.92, 353.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2094)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699742 reads in 184.330 CPU sec, 6.004 real sec
[M::process] read 697138 sequences (100000248 bp) in 1.935 CPU sec, 1.986 real sec...
[M::mem_process_seqs] Processed 699168 reads in 119.215 CPU sec, 3.829 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 291392, 32, 74)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 315, 818)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2124)
[M::mem_pestat] mean and std.dev: (452.20, 418.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2777)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (271.05, 137.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (273, 943, 1748)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4698)
[M::mem_pestat] mean and std.dev: (907.63, 808.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6173)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 247, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (314.40, 195.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1394)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 49, #reads = 1089029 mem = 536880478 peak = 1012183040
[M::process] read 697238 sequences (100000275 bp) in 2.837 CPU sec, 3.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 290853, 31, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 316, 1023)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579)
[M::mem_pestat] mean and std.dev: (520.22, 596.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3357)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 245, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (271.82, 137.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 316, 1517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4153)
[M::mem_pestat] mean and std.dev: (552.00, 665.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5471)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 234, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (303.03, 172.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697982 reads in 189.925 CPU sec, 6.213 real sec
[M::mem_process_seqs] Processed 697138 reads in 121.136 CPU sec, 3.955 real sec
[M::process] read 697008 sequences (100000162 bp) in 2.260 CPU sec, 2.488 real sec...
sorting block 50, #reads = 1087237 mem = 536879149 peak = 1012183040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 290327, 22, 68)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 297, 693)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1731)
[M::mem_pestat] mean and std.dev: (476.87, 433.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2250)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (270.89, 136.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 368, 974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2640)
[M::mem_pestat] mean and std.dev: (482.10, 446.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3473)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 318, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477)
[M::mem_pestat] mean and std.dev: (387.30, 306.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1905)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697256 sequences (100000023 bp) in 2.278 CPU sec, 2.783 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 290810, 24, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244)
[M::mem_pestat] mean and std.dev: (330.41, 269.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (271.21, 136.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 568, 1397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3735)
[M::mem_pestat] mean and std.dev: (729.36, 699.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4904)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 315, 570)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334)
[M::mem_pestat] mean and std.dev: (327.18, 226.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1716)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697238 reads in 199.203 CPU sec, 6.513 real sec
[M::process] read 705072 sequences (100000044 bp) in 2.282 CPU sec, 2.454 real sec...
[M::mem_process_seqs] Processed 697008 reads in 129.690 CPU sec, 4.213 real sec
sorting block 51, #reads = 1085721 mem = 536877935 peak = 1012183040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 290925, 28, 79)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 248, 659)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637)
[M::mem_pestat] mean and std.dev: (403.49, 363.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2126)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (271.70, 138.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (54, 530, 1481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4335)
[M::mem_pestat] mean and std.dev: (462.65, 492.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5762)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 308, 785)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1921)
[M::mem_pestat] mean and std.dev: (488.48, 441.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2489)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 705370 sequences (100000270 bp) in 2.509 CPU sec, 2.909 real sec...
sorting block 52, #reads = 1082888 mem = 536879749 peak = 1012183040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 293500, 20, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 256, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1422)
[M::mem_pestat] mean and std.dev: (387.28, 284.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1835)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 222, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (248.54, 125.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (342, 698, 1219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2973)
[M::mem_pestat] mean and std.dev: (800.00, 591.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3850)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 314, 1309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3533)
[M::mem_pestat] mean and std.dev: (633.17, 689.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4645)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697256 reads in 201.423 CPU sec, 6.582 real sec
[M::process] read 704818 sequences (100000053 bp) in 2.290 CPU sec, 2.500 real sec...
[M::mem_process_seqs] Processed 705072 reads in 134.475 CPU sec, 4.372 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 294332, 15, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 295, 844)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172)
[M::mem_pestat] mean and std.dev: (436.02, 379.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 221, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.17, 125.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 459, 872)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2198)
[M::mem_pestat] mean and std.dev: (364.38, 290.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2861)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 281, 731)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1753)
[M::mem_pestat] mean and std.dev: (389.56, 334.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2264)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 53, #reads = 1081440 mem = 536876731 peak = 1012183040
[M::process] read 704062 sequences (100000230 bp) in 2.379 CPU sec, 2.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 294267, 24, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 332, 1066)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2792)
[M::mem_pestat] mean and std.dev: (596.15, 610.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3655)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 222, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.99, 125.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (479, 814, 1669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4049)
[M::mem_pestat] mean and std.dev: (960.27, 811.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5239)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 223, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108)
[M::mem_pestat] mean and std.dev: (308.02, 218.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1423)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 705370 reads in 192.519 CPU sec, 6.312 real sec
[M::process] read 701996 sequences (100000090 bp) in 2.126 CPU sec, 2.151 real sec...
[M::mem_process_seqs] Processed 704818 reads in 126.109 CPU sec, 4.088 real sec
sorting block 54, #reads = 1089364 mem = 536877577 peak = 1012183040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 293975, 19, 68)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 248, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064)
[M::mem_pestat] mean and std.dev: (299.58, 235.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 224, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (251.40, 127.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 467, 845)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2051)
[M::mem_pestat] mean and std.dev: (548.00, 449.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2654)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 389, 711)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689)
[M::mem_pestat] mean and std.dev: (444.00, 323.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2178)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700502 sequences (100000098 bp) in 2.950 CPU sec, 3.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 292764, 27, 66)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 237, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209)
[M::mem_pestat] mean and std.dev: (287.76, 221.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 230, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (258.31, 131.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (505, 763, 1833)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4489)
[M::mem_pestat] mean and std.dev: (695.00, 464.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5817)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 351, 1071)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2811)
[M::mem_pestat] mean and std.dev: (535.19, 515.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3681)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698938 sequences (100000086 bp) in 1.948 CPU sec, 1.962 real sec...
[M::mem_process_seqs] Processed 704062 reads in 192.163 CPU sec, 6.157 real sec
[M::mem_process_seqs] Processed 701996 reads in 125.279 CPU sec, 3.978 real sec
sorting block 55, #reads = 1087245 mem = 536880110 peak = 1012183040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 292444, 23, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 286, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844)
[M::mem_pestat] mean and std.dev: (373.41, 359.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2400)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 235, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (264.08, 135.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (263, 702, 1200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3074)
[M::mem_pestat] mean and std.dev: (707.45, 525.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4011)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 409, 739)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839)
[M::mem_pestat] mean and std.dev: (442.45, 351.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2389)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697960 sequences (100000077 bp) in 2.492 CPU sec, 2.654 real sec...
[M::process] read 696982 sequences (100000221 bp) in 1.856 CPU sec, 1.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291404, 26, 88)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 304, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126)
[M::mem_pestat] mean and std.dev: (300.20, 178.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1445)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 241, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (268.64, 137.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (336, 1135, 1314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270)
[M::mem_pestat] mean and std.dev: (980.80, 784.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4248)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 254, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354)
[M::mem_pestat] mean and std.dev: (314.91, 235.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1755)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700502 reads in 190.215 CPU sec, 6.093 real sec
sorting block 56, #reads = 1086664 mem = 536880250 peak = 1012183040
[M::mem_process_seqs] Processed 698938 reads in 123.506 CPU sec, 3.937 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 291173, 32, 87)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 285, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330)
[M::mem_pestat] mean and std.dev: (370.56, 287.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
sorting block 57, #reads = 1086000 mem = 536879139 peak = 1061416960
[M::mem_pestat] mean and std.dev: (269.14, 136.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 551, 1200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3182)
[M::mem_pestat] mean and std.dev: (630.67, 486.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4173)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 310, 1253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3335)
[M::mem_pestat] mean and std.dev: (501.89, 533.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4376)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695962 sequences (100000063 bp) in 3.026 CPU sec, 3.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 290862, 34, 66)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 279, 772)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2022)
[M::mem_pestat] mean and std.dev: (477.60, 422.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2647)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 245, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (271.57, 137.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 508, 1329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3545)
[M::mem_pestat] mean and std.dev: (973.88, 1120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5458)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435)
[M::mem_pestat] mean and std.dev: (384.00, 281.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1846)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 695958 sequences (100000114 bp) in 1.947 CPU sec, 1.972 real sec...
[M::mem_process_seqs] Processed 697960 reads in 181.561 CPU sec, 5.794 real sec
[M::mem_process_seqs] Processed 696982 reads in 116.421 CPU sec, 3.694 real sec
[M::process] read 52768 sequences (7557815 bp) in 0.375 CPU sec, 0.385 real sec...
sorting block 58, #reads = 1085595 mem = 536883924 peak = 1061416960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 290375, 27, 74)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 285, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (300.60, 165.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (274.31, 137.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (322, 629, 1333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3355)
[M::mem_pestat] mean and std.dev: (784.42, 804.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4366)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 293, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (305.03, 132.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 22019, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 239, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (265.64, 133.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 290547, 26, 86)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 258, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140)
[M::mem_pestat] mean and std.dev: (285.78, 135.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1459)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (275.36, 139.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (285, 739, 1960)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5310)
[M::mem_pestat] mean and std.dev: (1142.39, 1426.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6985)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 267, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185)
[M::mem_pestat] mean and std.dev: (308.00, 205.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1523)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695962 reads in 169.084 CPU sec, 5.499 real sec
[M::mem_process_seqs] Processed 52768 reads in 95.189 CPU sec, 3.079 real sec
[M::mem_process_seqs] Processed 695958 reads in 111.619 CPU sec, 3.632 real sec
sorting block 59, #reads = 1085999 mem = 536878989 peak = 1061416960
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 65691956
overall: 69965422592 mem 11040.623 user 449.583 sys 401.749 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_27F-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1136_27F-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_27F-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 401.785 sec; CPU: 11490.209 sec
sorting block 60, #reads = 964735 mem = 477223323 peak = 1061416960
uniq header count 1
merging 61 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3662157949
output reads: 66247565
bam_mem_sort: 61 calls 41.682 user 0.785 sys 43.733 real
bam_write: 61 calls 39.696 user 2.750 sys 75.966 real
execute: 1 calls 19.873 user 1.686 sys 425.936 real
merge_files: 1 calls 19.853 user 1.654 sys 23.201 real
parse_chunk: 975 calls 46.217 user 11.442 sys 70.667 real
read_chunk: 2634 calls 23.919 user 17.440 sys 328.551 real
sort_block: 1 calls 16.903 user 16.544 sys 318.047 real
write_chunk: 1657 calls 19.489 user 1.433 sys 22.354 real
overall: 2804330496 mem 906.196 user 48.975 sys 425.961 real
ls: cannot access '*.cram': No such file or directory