.command.log - Workdir - DAQ:SENTIEON_BWAMEM (659_Kh-T1-TDNA-1_A23MHWWLT4_1;L005;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f3/4bc2f2673054f0506a6925656fdaac/.command.log
Size: 173.6 KB
Task: DAQ:SENTIEON_BWAMEM (659_Kh-T1-TDNA-1_A23MHWWLT4_1;L005;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f3/4bc2f2673054f0506a6925656fdaac/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a5/0474d5e5812fb26c4a26ab48add794/659_Kh-T1-TDNA-1_A23MHWWLT4_1/659_Kh-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f3/4bc2f2673054f0506a6925656fdaac/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a5/0474d5e5812fb26c4a26ab48add794/659_Kh-T1-TDNA-1_A23MHWWLT4_1/659_Kh-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_Kh-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1882.806 CPU sec, 71.574 real sec
[M::process] read 673538 sequences (100000154 bp) in 1.062 CPU sec, 1.068 real sec...
[M::process] read 672708 sequences (100000053 bp) in 1.559 CPU sec, 1.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278641, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 346, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (365.88, 160.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672236 sequences (100000098 bp) in 1.611 CPU sec, 1.621 real sec...
[M::process] read 672242 sequences (100000169 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278430, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 355, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (373.64, 161.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673538 reads in 180.663 CPU sec, 5.685 real sec
[M::mem_process_seqs] Processed 672708 reads in 114.545 CPU sec, 3.634 real sec
sorting block 0, #reads = 1075990 mem = 536877962 peak = 598421504
[M::process] read 672084 sequences (100000233 bp) in 1.832 CPU sec, 1.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278520, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 362, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (378.49, 162.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671946 sequences (100000033 bp) in 1.520 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 278421, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 157, 1038)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2824)
[M::mem_pestat] mean and std.dev: (401.08, 373.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3717)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 367, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (382.06, 162.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278486, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 369, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (382.58, 162.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672236 reads in 231.475 CPU sec, 7.401 real sec
[M::mem_process_seqs] Processed 672242 reads in 170.308 CPU sec, 5.482 real sec
[M::mem_process_seqs] Processed 672084 reads in 184.981 CPU sec, 5.964 real sec
sorting block 1, #reads = 1075037 mem = 536877777 peak = 725823488
sorting block 2, #reads = 1075103 mem = 536878173 peak = 855986176
[M::process] read 671604 sequences (100000234 bp) in 2.080 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278715, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 371, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (385.64, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671394 sequences (100000087 bp) in 1.623 CPU sec, 1.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 278014, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (388.94, 164.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671278 sequences (100000135 bp) in 1.468 CPU sec, 1.486 real sec...
[M::mem_process_seqs] Processed 671946 reads in 170.990 CPU sec, 5.409 real sec
[M::mem_process_seqs] Processed 671604 reads in 106.795 CPU sec, 3.372 real sec
sorting block 3, #reads = 1075493 mem = 536880167 peak = 855986176
[M::process] read 671064 sequences (100000232 bp) in 2.096 CPU sec, 2.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277570, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 376, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (389.36, 164.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671042 sequences (100000173 bp) in 1.700 CPU sec, 1.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278027, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 378, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (391.99, 164.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671394 reads in 166.447 CPU sec, 5.278 real sec
[M::mem_process_seqs] Processed 671278 reads in 104.021 CPU sec, 3.315 real sec
sorting block 4, #reads = 1075501 mem = 536881066 peak = 855986176
[M::process] read 670952 sequences (100000049 bp) in 1.843 CPU sec, 1.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277759, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (393.44, 164.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670866 sequences (100000183 bp) in 1.340 CPU sec, 1.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278058, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 383, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (395.85, 165.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277647, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (393.86, 164.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671064 reads in 255.362 CPU sec, 8.201 real sec
sorting block 5, #reads = 1074932 mem = 536878263 peak = 855986176
[M::mem_process_seqs] Processed 670952 reads in 191.798 CPU sec, 6.182 real sec
[M::mem_process_seqs] Processed 671042 reads in 209.126 CPU sec, 6.759 real sec
sorting block 6, #reads = 1074232 mem = 536883480 peak = 855986176
[M::process] read 670712 sequences (100000064 bp) in 2.713 CPU sec, 2.889 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277592, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (396.11, 165.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670844 sequences (100000258 bp) in 1.971 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277685, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 386, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (398.71, 166.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670866 reads in 187.016 CPU sec, 5.957 real sec
[M::process] read 670668 sequences (100000160 bp) in 1.830 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 670712 reads in 118.387 CPU sec, 3.721 real sec
sorting block 7, #reads = 1074074 mem = 536878305 peak = 855986176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 276878, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 383, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (395.66, 164.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670626 sequences (100000166 bp) in 2.158 CPU sec, 2.250 real sec...
[M::process] read 670492 sequences (100000227 bp) in 1.670 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277810, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 384, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (396.27, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670844 reads in 179.285 CPU sec, 5.701 real sec
sorting block 8, #reads = 1073818 mem = 536877938 peak = 855986176
[M::mem_process_seqs] Processed 670668 reads in 112.535 CPU sec, 3.574 real sec
sorting block 9, #reads = 1073614 mem = 536879785 peak = 855986176
[M::process] read 670276 sequences (100000059 bp) in 2.067 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277602, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 382, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (395.08, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670558 sequences (100000206 bp) in 1.699 CPU sec, 1.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277101, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 378, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (391.86, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277555, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 382, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (394.83, 162.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670626 reads in 262.664 CPU sec, 8.404 real sec
[M::mem_process_seqs] Processed 670492 reads in 195.219 CPU sec, 6.260 real sec
[M::mem_process_seqs] Processed 670276 reads in 210.857 CPU sec, 6.776 real sec
sorting block 10, #reads = 1074411 mem = 536879056 peak = 855986176
[M::process] read 670460 sequences (100000191 bp) in 2.621 CPU sec, 2.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277342, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 383, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (395.88, 163.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670796 sequences (100000044 bp) in 1.861 CPU sec, 1.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277975, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 377, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (391.03, 161.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670558 reads in 188.395 CPU sec, 5.995 real sec
[M::process] read 670810 sequences (100000109 bp) in 1.643 CPU sec, 1.646 real sec...
sorting block 11, #reads = 1073809 mem = 536878356 peak = 855986176
[M::mem_process_seqs] Processed 670460 reads in 117.888 CPU sec, 3.727 real sec
sorting block 12, #reads = 1073474 mem = 536886303 peak = 906477568
[M::process] read 670800 sequences (100000131 bp) in 2.088 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277516, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 371, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (385.60, 159.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670454 sequences (100000161 bp) in 1.745 CPU sec, 1.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277826, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 372, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (387.34, 160.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278143, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 375, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (388.49, 160.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670796 reads in 268.424 CPU sec, 8.594 real sec
[M::mem_process_seqs] Processed 670810 reads in 198.029 CPU sec, 6.361 real sec
[M::mem_process_seqs] Processed 670800 reads in 214.615 CPU sec, 6.911 real sec
sorting block 13, #reads = 1072373 mem = 536879564 peak = 906477568
[M::process] read 670390 sequences (100000276 bp) in 2.450 CPU sec, 2.616 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278083, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (398.77, 165.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1071641 mem = 536882350 peak = 906477568
[M::process] read 670402 sequences (100000298 bp) in 2.016 CPU sec, 2.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277913, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (399.13, 166.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670454 reads in 187.713 CPU sec, 5.959 real sec
[M::process] read 671230 sequences (100000205 bp) in 2.001 CPU sec, 2.032 real sec...
[M::mem_process_seqs] Processed 670390 reads in 118.738 CPU sec, 3.734 real sec
sorting block 15, #reads = 1072275 mem = 536877701 peak = 906477568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278104, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 387, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (401.49, 168.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672896 sequences (100000066 bp) in 2.222 CPU sec, 2.332 real sec...
[M::process] read 672396 sequences (100000257 bp) in 1.539 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278275, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (394.31, 169.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670402 reads in 187.848 CPU sec, 5.998 real sec
[M::mem_process_seqs] Processed 671230 reads in 117.702 CPU sec, 3.759 real sec
sorting block 16, #reads = 1072024 mem = 536881345 peak = 906477568
[M::process] read 672046 sequences (100000167 bp) in 2.205 CPU sec, 2.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277992, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 355, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (375.42, 164.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671926 sequences (100000054 bp) in 1.810 CPU sec, 1.809 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278365, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 364, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (381.76, 165.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672896 reads in 184.945 CPU sec, 5.879 real sec
[M::mem_process_seqs] Processed 672396 reads in 114.714 CPU sec, 3.647 real sec
sorting block 17, #reads = 1073989 mem = 536879855 peak = 906477568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278043, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 367, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (382.15, 163.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671920 sequences (100000231 bp) in 2.219 CPU sec, 2.318 real sec...
[M::process] read 671660 sequences (100000028 bp) in 1.810 CPU sec, 1.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277965, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 368, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (383.22, 163.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672046 reads in 170.397 CPU sec, 5.408 real sec
sorting block 18, #reads = 1077002 mem = 536878702 peak = 906477568
[M::mem_process_seqs] Processed 671926 reads in 106.855 CPU sec, 3.386 real sec
sorting block 19, #reads = 1076097 mem = 536878948 peak = 918454272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278406, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 369, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (384.45, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671274 sequences (100000021 bp) in 2.282 CPU sec, 2.374 real sec...
[M::process] read 671354 sequences (100000062 bp) in 1.820 CPU sec, 1.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278315, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 371, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (385.95, 163.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671920 reads in 172.383 CPU sec, 5.668 real sec
[M::mem_process_seqs] Processed 671660 reads in 106.348 CPU sec, 3.370 real sec
sorting block 20, #reads = 1075249 mem = 536879747 peak = 918454272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277898, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 378, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (391.65, 165.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671426 sequences (100000190 bp) in 2.215 CPU sec, 2.316 real sec...
[M::process] read 671138 sequences (100000188 bp) in 1.830 CPU sec, 1.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278008, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (392.39, 166.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671274 reads in 173.033 CPU sec, 5.492 real sec
[M::mem_process_seqs] Processed 671354 reads in 113.775 CPU sec, 3.617 real sec
sorting block 21, #reads = 1075793 mem = 536878496 peak = 918454272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277625, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 376, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (389.65, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671176 sequences (100000115 bp) in 2.230 CPU sec, 2.319 real sec...
[M::process] read 671074 sequences (100000261 bp) in 1.470 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277370, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 376, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (389.74, 163.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671426 reads in 173.930 CPU sec, 5.536 real sec
[M::mem_process_seqs] Processed 671138 reads in 107.877 CPU sec, 3.431 real sec
sorting block 22, #reads = 1075942 mem = 536877337 peak = 918454272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277862, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 378, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (391.59, 163.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670988 sequences (100000197 bp) in 2.237 CPU sec, 2.347 real sec...
[M::process] read 670822 sequences (100000224 bp) in 1.480 CPU sec, 1.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277364, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (392.74, 164.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671176 reads in 175.030 CPU sec, 5.569 real sec
sorting block 23, #reads = 1075944 mem = 536879327 peak = 918454272
[M::mem_process_seqs] Processed 671074 reads in 108.151 CPU sec, 3.445 real sec
sorting block 24, #reads = 1075414 mem = 536879980 peak = 949911552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277674, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (395.47, 165.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670902 sequences (100000156 bp) in 2.347 CPU sec, 2.450 real sec...
[M::process] read 670700 sequences (100000193 bp) in 1.860 CPU sec, 1.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277203, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (394.75, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670988 reads in 173.996 CPU sec, 5.517 real sec
[M::mem_process_seqs] Processed 670822 reads in 110.462 CPU sec, 3.499 real sec
sorting block 25, #reads = 1074149 mem = 536880386 peak = 949911552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 277760, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (397.13, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670830 sequences (100000099 bp) in 2.340 CPU sec, 2.436 real sec...
[M::process] read 670568 sequences (100000030 bp) in 1.868 CPU sec, 1.869 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278291, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (393.56, 163.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670902 reads in 176.766 CPU sec, 5.608 real sec
[M::mem_process_seqs] Processed 670700 reads in 111.668 CPU sec, 3.538 real sec
sorting block 26, #reads = 1074777 mem = 536878532 peak = 949911552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277263, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 381, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (394.48, 164.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670742 sequences (100000229 bp) in 2.296 CPU sec, 2.423 real sec...
[M::process] read 670728 sequences (100000101 bp) in 1.850 CPU sec, 1.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 276986, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 382, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (395.73, 165.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670830 reads in 179.900 CPU sec, 5.713 real sec
[M::mem_process_seqs] Processed 670568 reads in 113.842 CPU sec, 3.604 real sec
sorting block 27, #reads = 1073435 mem = 536877554 peak = 949911552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277895, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 380, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (394.40, 164.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670834 sequences (100000267 bp) in 2.308 CPU sec, 2.388 real sec...
[M::process] read 670908 sequences (100000029 bp) in 1.710 CPU sec, 1.708 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277921, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 378, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (393.02, 164.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670742 reads in 174.221 CPU sec, 5.534 real sec
sorting block 28, #reads = 1074098 mem = 536878922 peak = 949911552
[M::mem_process_seqs] Processed 670728 reads in 116.601 CPU sec, 3.710 real sec
sorting block 29, #reads = 1074333 mem = 536882482 peak = 956989440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277952, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (393.19, 165.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670696 sequences (100000183 bp) in 2.349 CPU sec, 2.450 real sec...
[M::process] read 670748 sequences (100000181 bp) in 1.860 CPU sec, 1.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277944, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 376, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (390.40, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670834 reads in 174.773 CPU sec, 5.552 real sec
[M::mem_process_seqs] Processed 670908 reads in 109.263 CPU sec, 3.462 real sec
sorting block 30, #reads = 1073001 mem = 536878077 peak = 956989440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277346, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 374, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (389.26, 163.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670602 sequences (100000182 bp) in 2.379 CPU sec, 2.460 real sec...
[M::process] read 670412 sequences (100000299 bp) in 1.560 CPU sec, 1.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277970, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 378, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (393.40, 165.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670696 reads in 173.101 CPU sec, 5.494 real sec
[M::mem_process_seqs] Processed 670748 reads in 109.233 CPU sec, 3.466 real sec
sorting block 31, #reads = 1074128 mem = 536879223 peak = 956989440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277995, 10, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (395.36, 166.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (498, 498, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (498, 498)
[M::mem_pestat] mean and std.dev: (498.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (498, 498)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 670342 sequences (100000294 bp) in 2.377 CPU sec, 2.469 real sec...
[M::process] read 671580 sequences (100000169 bp) in 1.870 CPU sec, 1.869 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278239, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 387, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (402.06, 169.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670602 reads in 168.906 CPU sec, 5.352 real sec
sorting block 32, #reads = 1074260 mem = 536876235 peak = 956989440
[M::mem_process_seqs] Processed 670412 reads in 106.610 CPU sec, 3.373 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278042, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 389, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (403.96, 171.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673070 sequences (100000086 bp) in 2.064 CPU sec, 2.150 real sec...
[M::process] read 672178 sequences (100000224 bp) in 1.537 CPU sec, 1.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278515, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 370, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (388.34, 168.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670342 reads in 169.092 CPU sec, 5.382 real sec
sorting block 33, #reads = 1073523 mem = 536879426 peak = 956989440
[M::mem_process_seqs] Processed 671580 reads in 108.140 CPU sec, 3.436 real sec
sorting block 34, #reads = 1073228 mem = 536880720 peak = 963186688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279308, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (371.50, 164.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671816 sequences (100000047 bp) in 2.438 CPU sec, 2.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279219, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 363, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (380.87, 165.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671526 sequences (100000117 bp) in 1.656 CPU sec, 1.666 real sec...
[M::mem_process_seqs] Processed 673070 reads in 166.185 CPU sec, 5.282 real sec
[M::process] read 671392 sequences (100000161 bp) in 1.530 CPU sec, 1.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278758, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 369, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (385.37, 165.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671816 reads in 97.319 CPU sec, 3.304 real sec
sorting block 35, #reads = 1075447 mem = 536878942 peak = 963186688
[M::mem_process_seqs] Processed 672178 reads in 230.670 CPU sec, 7.544 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278187, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 371, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (386.82, 165.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1075829 mem = 536880856 peak = 963186688
[M::process] read 671300 sequences (100000060 bp) in 2.245 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278275, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (390.59, 166.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671222 sequences (100000230 bp) in 1.726 CPU sec, 1.735 real sec...
[M::mem_process_seqs] Processed 671526 reads in 187.583 CPU sec, 5.963 real sec
[M::mem_process_seqs] Processed 671392 reads in 110.173 CPU sec, 3.482 real sec
sorting block 37, #reads = 1075633 mem = 536880697 peak = 963186688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278858, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (394.76, 168.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671146 sequences (100000116 bp) in 2.573 CPU sec, 2.732 real sec...
[M::process] read 671016 sequences (100000275 bp) in 1.771 CPU sec, 1.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278208, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (396.61, 168.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671300 reads in 170.206 CPU sec, 5.400 real sec
[M::mem_process_seqs] Processed 671222 reads in 108.881 CPU sec, 3.453 real sec
sorting block 38, #reads = 1075589 mem = 536878412 peak = 963186688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278247, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (399.63, 171.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670792 sequences (100000046 bp) in 2.233 CPU sec, 2.323 real sec...
[M::process] read 670698 sequences (100000112 bp) in 1.873 CPU sec, 1.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278131, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 386, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (401.16, 171.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671146 reads in 168.504 CPU sec, 5.346 real sec
[M::mem_process_seqs] Processed 671016 reads in 106.423 CPU sec, 3.371 real sec
sorting block 39, #reads = 1074159 mem = 536877399 peak = 963186688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278070, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 388, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (401.99, 170.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671398 sequences (100000182 bp) in 2.628 CPU sec, 2.727 real sec...
[M::process] read 670686 sequences (100000022 bp) in 1.835 CPU sec, 1.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277622, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (402.14, 170.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670792 reads in 170.725 CPU sec, 5.410 real sec
sorting block 40, #reads = 1075365 mem = 536881308 peak = 963186688
[M::mem_process_seqs] Processed 670698 reads in 110.022 CPU sec, 3.480 real sec
sorting block 41, #reads = 1074466 mem = 536878034 peak = 976154624
[M::process] read 670492 sequences (100000100 bp) in 2.299 CPU sec, 2.448 real sec...
[M::process] read 670438 sequences (100000182 bp) in 1.736 CPU sec, 1.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278259, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (402.51, 171.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670686 reads in 96.211 CPU sec, 3.049 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278415, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 378, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (395.14, 170.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277888, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (403.67, 170.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670578 sequences (100000267 bp) in 1.652 CPU sec, 1.662 real sec...
[M::mem_process_seqs] Processed 671398 reads in 266.561 CPU sec, 8.476 real sec
sorting block 42, #reads = 1075278 mem = 536876399 peak = 976154624
[M::mem_process_seqs] Processed 670492 reads in 125.907 CPU sec, 4.014 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277740, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (399.63, 168.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670400 sequences (100000211 bp) in 2.311 CPU sec, 2.477 real sec...
[M::process] read 670598 sequences (100000161 bp) in 1.301 CPU sec, 1.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278171, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (402.58, 169.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670438 reads in 170.846 CPU sec, 5.447 real sec
sorting block 43, #reads = 1075119 mem = 536886682 peak = 976154624
[M::mem_process_seqs] Processed 670578 reads in 107.523 CPU sec, 3.425 real sec
sorting block 44, #reads = 1074332 mem = 536880430 peak = 1004498944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278762, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (400.94, 169.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670580 sequences (100000008 bp) in 2.477 CPU sec, 2.578 real sec...
[M::process] read 670676 sequences (100000181 bp) in 1.779 CPU sec, 1.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278379, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (398.54, 169.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670400 reads in 164.099 CPU sec, 5.193 real sec
[M::mem_process_seqs] Processed 670598 reads in 102.391 CPU sec, 3.237 real sec
sorting block 45, #reads = 1074716 mem = 536879664 peak = 1004498944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278036, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (398.55, 170.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670954 sequences (100000111 bp) in 2.295 CPU sec, 2.389 real sec...
[M::process] read 670650 sequences (100000278 bp) in 1.739 CPU sec, 1.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277926, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (397.04, 169.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670580 reads in 173.396 CPU sec, 5.512 real sec
[M::mem_process_seqs] Processed 670676 reads in 112.265 CPU sec, 3.554 real sec
sorting block 46, #reads = 1075429 mem = 536878711 peak = 1004498944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278844, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (398.06, 171.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670486 sequences (100000284 bp) in 2.290 CPU sec, 2.420 real sec...
[M::process] read 670256 sequences (100000076 bp) in 1.495 CPU sec, 1.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277968, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (399.16, 170.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670954 reads in 173.408 CPU sec, 5.516 real sec
sorting block 47, #reads = 1073598 mem = 536878724 peak = 1004498944
[M::mem_process_seqs] Processed 670650 reads in 110.055 CPU sec, 3.494 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278225, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (398.27, 168.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670410 sequences (100000179 bp) in 2.602 CPU sec, 2.693 real sec...
[M::process] read 670580 sequences (100000184 bp) in 1.490 CPU sec, 1.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278584, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (398.89, 168.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670486 reads in 171.031 CPU sec, 5.429 real sec
sorting block 48, #reads = 1074508 mem = 536878403 peak = 1004498944
[M::mem_process_seqs] Processed 670256 reads in 108.040 CPU sec, 3.427 real sec
sorting block 49, #reads = 1073771 mem = 536882847 peak = 1019326464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278326, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (402.40, 170.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670372 sequences (100000172 bp) in 2.427 CPU sec, 2.536 real sec...
[M::process] read 672602 sequences (100000275 bp) in 1.889 CPU sec, 1.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278373, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (404.35, 172.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670410 reads in 168.991 CPU sec, 5.355 real sec
[M::mem_process_seqs] Processed 670580 reads in 107.308 CPU sec, 3.393 real sec
sorting block 50, #reads = 1074073 mem = 536878913 peak = 1019326464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278643, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 394, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (408.91, 174.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673046 sequences (100000005 bp) in 2.455 CPU sec, 2.556 real sec...
[M::process] read 671648 sequences (100000022 bp) in 1.530 CPU sec, 1.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279088, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 364, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (383.55, 169.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670372 reads in 173.877 CPU sec, 5.531 real sec
[M::mem_process_seqs] Processed 672602 reads in 112.836 CPU sec, 3.587 real sec
sorting block 51, #reads = 1072843 mem = 536879604 peak = 1019326464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278514, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (376.81, 168.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671348 sequences (100000022 bp) in 2.681 CPU sec, 2.797 real sec...
[M::process] read 671282 sequences (100000129 bp) in 1.689 CPU sec, 1.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278744, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 373, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (392.12, 171.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673046 reads in 173.377 CPU sec, 5.502 real sec
sorting block 52, #reads = 1075192 mem = 536879664 peak = 1019326464
[M::mem_process_seqs] Processed 671648 reads in 108.732 CPU sec, 3.444 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277477, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 377, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (394.55, 171.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671068 sequences (100000032 bp) in 2.501 CPU sec, 2.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278023, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 671066 sequences (100000244 bp) in 1.768 CPU sec, 1.770 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (265, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (397.39, 171.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671348 reads in 170.942 CPU sec, 5.433 real sec
sorting block 53, #reads = 1077018 mem = 536878072 peak = 1019326464
[M::mem_process_seqs] Processed 671282 reads in 107.415 CPU sec, 3.399 real sec
sorting block 54, #reads = 1076032 mem = 536879281 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 277714, 0, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (2819, 2819, 2819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2819, 2819)
[M::mem_pestat] mean and std.dev: (2819.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (2819, 2819)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (398.94, 171.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 670638 sequences (100000253 bp) in 2.649 CPU sec, 2.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278677, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 670660 sequences (100000165 bp) in 1.820 CPU sec, 1.826 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (400.02, 171.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (375, 1244, 5393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15429)
[M::mem_pestat] mean and std.dev: (1980.55, 2120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20447)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 671068 reads in 168.627 CPU sec, 5.348 real sec
[M::mem_process_seqs] Processed 671066 reads in 107.472 CPU sec, 3.395 real sec
sorting block 55, #reads = 1076209 mem = 536876336 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277941, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 389, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (404.48, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670668 sequences (100000080 bp) in 2.717 CPU sec, 2.834 real sec...
[M::process] read 670550 sequences (100000059 bp) in 1.830 CPU sec, 1.836 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277775, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 391, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (405.14, 173.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670638 reads in 171.517 CPU sec, 5.440 real sec
[M::mem_process_seqs] Processed 670660 reads in 105.909 CPU sec, 3.351 real sec
sorting block 56, #reads = 1076305 mem = 536882342 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278104, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (404.60, 172.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670610 sequences (100000228 bp) in 2.331 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278080, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 391, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (405.60, 172.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670668 reads in 143.397 CPU sec, 4.544 real sec
[M::process] read 671140 sequences (100000001 bp) in 1.793 CPU sec, 1.795 real sec...
[M::mem_process_seqs] Processed 670550 reads in 89.342 CPU sec, 2.804 real sec
sorting block 57, #reads = 1075531 mem = 536877555 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278503, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 390, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (404.99, 171.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670432 sequences (100000258 bp) in 2.535 CPU sec, 2.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278353, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (398.18, 171.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670610 reads in 143.983 CPU sec, 4.555 real sec
sorting block 58, #reads = 1076228 mem = 536877887 peak = 1052676096
[M::process] read 670364 sequences (100000055 bp) in 1.737 CPU sec, 1.742 real sec...
[M::mem_process_seqs] Processed 671140 reads in 90.831 CPU sec, 2.855 real sec
sorting block 59, #reads = 1075058 mem = 536878462 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277582, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (405.02, 171.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670532 sequences (100000053 bp) in 2.616 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277646, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 392, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (405.70, 171.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670402 sequences (100000115 bp) in 1.780 CPU sec, 1.778 real sec...
[M::mem_process_seqs] Processed 670432 reads in 158.328 CPU sec, 5.017 real sec
[M::mem_process_seqs] Processed 670364 reads in 99.934 CPU sec, 3.153 real sec
sorting block 60, #reads = 1074587 mem = 536878373 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278357, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (400.20, 168.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670378 sequences (100000068 bp) in 2.384 CPU sec, 2.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278433, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (397.73, 166.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670636 sequences (100000078 bp) in 1.760 CPU sec, 1.761 real sec...
[M::mem_process_seqs] Processed 670532 reads in 154.821 CPU sec, 4.911 real sec
[M::mem_process_seqs] Processed 670402 reads in 96.271 CPU sec, 3.042 real sec
sorting block 61, #reads = 1074412 mem = 536880299 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278143, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (394.67, 164.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670560 sequences (100000131 bp) in 2.582 CPU sec, 2.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278578, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (392.25, 164.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670656 sequences (100000096 bp) in 1.750 CPU sec, 1.751 real sec...
[M::mem_process_seqs] Processed 670378 reads in 150.552 CPU sec, 4.761 real sec
sorting block 62, #reads = 1074841 mem = 536885226 peak = 1052676096
[M::process] read 670614 sequences (100000279 bp) in 1.606 CPU sec, 1.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278191, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (394.29, 166.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670560 reads in 87.325 CPU sec, 2.976 real sec
[M::mem_process_seqs] Processed 670636 reads in 203.902 CPU sec, 6.660 real sec
sorting block 63, #reads = 1073677 mem = 536879753 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278171, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (396.00, 166.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670478 sequences (100000083 bp) in 2.492 CPU sec, 2.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278075, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 378, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (394.09, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670656 reads in 163.741 CPU sec, 5.194 real sec
[M::process] read 670564 sequences (100000257 bp) in 1.983 CPU sec, 2.042 real sec...
sorting block 64, #reads = 1074075 mem = 536878059 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278338, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (396.89, 167.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670492 sequences (100000138 bp) in 1.763 CPU sec, 1.764 real sec...
[M::mem_process_seqs] Processed 670478 reads in 91.034 CPU sec, 2.881 real sec
[M::mem_process_seqs] Processed 670614 reads in 209.832 CPU sec, 6.630 real sec
sorting block 65, #reads = 1074171 mem = 536880952 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277464, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (395.33, 166.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670266 sequences (100000059 bp) in 2.420 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278815, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (400.55, 168.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670564 reads in 170.934 CPU sec, 5.412 real sec
[M::process] read 670280 sequences (100000164 bp) in 1.890 CPU sec, 1.908 real sec...
[M::mem_process_seqs] Processed 670492 reads in 100.106 CPU sec, 3.141 real sec
sorting block 66, #reads = 1073203 mem = 536880933 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278507, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 388, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (403.27, 169.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670272 sequences (100000261 bp) in 2.185 CPU sec, 2.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278445, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (405.01, 171.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672990 sequences (100000096 bp) in 1.837 CPU sec, 1.836 real sec...
[M::mem_process_seqs] Processed 670266 reads in 156.645 CPU sec, 4.970 real sec
sorting block 67, #reads = 1073936 mem = 536882000 peak = 1052676096
[M::mem_process_seqs] Processed 670280 reads in 99.884 CPU sec, 3.150 real sec
sorting block 68, #reads = 1073277 mem = 536879943 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278412, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 390, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (405.48, 171.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674058 sequences (100000114 bp) in 2.425 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278811, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 359, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (379.48, 169.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673344 sequences (100000199 bp) in 1.840 CPU sec, 1.841 real sec...
[M::mem_process_seqs] Processed 670272 reads in 157.003 CPU sec, 4.968 real sec
[M::mem_process_seqs] Processed 672990 reads in 103.747 CPU sec, 3.267 real sec
sorting block 69, #reads = 1072291 mem = 536877979 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279836, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (369.58, 165.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673510 sequences (100000018 bp) in 2.186 CPU sec, 2.316 real sec...
[M::process] read 672706 sequences (100000207 bp) in 1.969 CPU sec, 1.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279626, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (375.68, 165.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674058 reads in 169.234 CPU sec, 5.373 real sec
[M::mem_process_seqs] Processed 673344 reads in 104.419 CPU sec, 3.304 real sec
sorting block 70, #reads = 1070813 mem = 536881110 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279530, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 358, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (374.61, 163.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672166 sequences (100000018 bp) in 2.545 CPU sec, 2.653 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279456, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 363, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (377.63, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672254 sequences (100000266 bp) in 1.870 CPU sec, 1.868 real sec...
[M::mem_process_seqs] Processed 673510 reads in 162.785 CPU sec, 5.149 real sec
[M::mem_process_seqs] Processed 672706 reads in 101.149 CPU sec, 3.194 real sec
sorting block 71, #reads = 1072310 mem = 536879014 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278237, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 370, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (385.25, 165.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672016 sequences (100000029 bp) in 2.337 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 278654, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (358, 3170, 3170)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8794)
[M::mem_pestat] mean and std.dev: (1764.00, 1406.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11606)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 372, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (385.77, 164.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 671810 sequences (100000012 bp) in 1.840 CPU sec, 1.846 real sec...
[M::mem_process_seqs] Processed 672166 reads in 159.511 CPU sec, 5.050 real sec
sorting block 72, #reads = 1072577 mem = 536881021 peak = 1052676096
[M::mem_process_seqs] Processed 672254 reads in 97.912 CPU sec, 3.085 real sec
sorting block 73, #reads = 1071050 mem = 536878295 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278603, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (388.07, 166.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671808 sequences (100000159 bp) in 2.096 CPU sec, 2.183 real sec...
[M::process] read 672046 sequences (100000074 bp) in 1.510 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278179, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (390.06, 166.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672016 reads in 163.585 CPU sec, 5.203 real sec
[M::mem_process_seqs] Processed 671810 reads in 103.636 CPU sec, 3.288 real sec
sorting block 74, #reads = 1072214 mem = 536878910 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278559, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (388.34, 164.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671794 sequences (100000159 bp) in 2.047 CPU sec, 2.132 real sec...
[M::process] read 671970 sequences (100000142 bp) in 1.440 CPU sec, 1.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278637, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (388.22, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671808 reads in 164.815 CPU sec, 5.246 real sec
[M::mem_process_seqs] Processed 672046 reads in 103.119 CPU sec, 3.276 real sec
sorting block 75, #reads = 1072183 mem = 536876215 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278300, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (389.38, 164.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671810 sequences (100000124 bp) in 2.464 CPU sec, 2.532 real sec...
[M::process] read 671548 sequences (100000167 bp) in 1.870 CPU sec, 1.878 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278766, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 376, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (387.29, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671794 reads in 168.199 CPU sec, 5.327 real sec
[M::mem_process_seqs] Processed 671970 reads in 103.971 CPU sec, 3.288 real sec
sorting block 76, #reads = 1071193 mem = 536878794 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278016, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (388.34, 164.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671726 sequences (100000035 bp) in 2.330 CPU sec, 2.421 real sec...
[M::process] read 671672 sequences (100000179 bp) in 1.870 CPU sec, 1.868 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278367, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (391.24, 165.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671810 reads in 165.658 CPU sec, 5.246 real sec
sorting block 77, #reads = 1070814 mem = 536880832 peak = 1052676096
[M::mem_process_seqs] Processed 671548 reads in 105.366 CPU sec, 3.329 real sec
sorting block 78, #reads = 1070684 mem = 536880342 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278278, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (391.98, 166.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671392 sequences (100000063 bp) in 2.608 CPU sec, 2.697 real sec...
[M::process] read 671466 sequences (100000251 bp) in 1.860 CPU sec, 1.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278627, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (390.57, 164.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671726 reads in 174.511 CPU sec, 5.528 real sec
[M::mem_process_seqs] Processed 671672 reads in 110.549 CPU sec, 3.498 real sec
sorting block 79, #reads = 1071454 mem = 536877437 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277908, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (389.50, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671276 sequences (100000075 bp) in 2.227 CPU sec, 2.314 real sec...
[M::process] read 671308 sequences (100000208 bp) in 1.430 CPU sec, 1.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278535, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (387.91, 161.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671392 reads in 180.884 CPU sec, 5.758 real sec
[M::mem_process_seqs] Processed 671466 reads in 114.277 CPU sec, 3.641 real sec
sorting block 80, #reads = 1070775 mem = 536882736 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278050, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 375, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (387.32, 160.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671000 sequences (100000150 bp) in 2.478 CPU sec, 2.562 real sec...
[M::process] read 670840 sequences (100000087 bp) in 1.820 CPU sec, 1.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277804, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 377, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (389.23, 161.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671276 reads in 180.134 CPU sec, 5.712 real sec
[M::mem_process_seqs] Processed 671308 reads in 112.828 CPU sec, 3.576 real sec
sorting block 81, #reads = 1070645 mem = 536879006 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278832, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (392.09, 162.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670978 sequences (100000153 bp) in 2.252 CPU sec, 2.328 real sec...
[M::process] read 670846 sequences (100000132 bp) in 1.640 CPU sec, 1.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278883, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 383, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (394.44, 162.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671000 reads in 178.090 CPU sec, 5.659 real sec
sorting block 82, #reads = 1070635 mem = 536878781 peak = 1052676096
[M::mem_process_seqs] Processed 670840 reads in 112.333 CPU sec, 3.563 real sec
sorting block 83, #reads = 1071196 mem = 536877493 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278444, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (393.43, 164.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670986 sequences (100000210 bp) in 2.250 CPU sec, 2.321 real sec...
[M::process] read 677556 sequences (100000140 bp) in 2.020 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278255, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (397.77, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670978 reads in 173.652 CPU sec, 5.503 real sec
[M::mem_process_seqs] Processed 670846 reads in 110.469 CPU sec, 3.498 real sec
sorting block 84, #reads = 1070074 mem = 536883206 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278580, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (396.92, 168.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676486 sequences (100000248 bp) in 2.509 CPU sec, 2.589 real sec...
[M::process] read 675688 sequences (100000017 bp) in 1.890 CPU sec, 1.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281166, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 311, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (332.38, 147.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670986 reads in 191.198 CPU sec, 6.065 real sec
[M::mem_process_seqs] Processed 677556 reads in 130.123 CPU sec, 4.125 real sec
sorting block 85, #reads = 1069278 mem = 536878421 peak = 1052676096
[M::process] read 674918 sequences (100000067 bp) in 2.242 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281017, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 324, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (343.41, 151.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673986 sequences (100000177 bp) in 1.490 CPU sec, 1.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280435, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (349.54, 153.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280497, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (355.88, 154.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676486 reads in 286.056 CPU sec, 9.145 real sec
sorting block 86, #reads = 1064149 mem = 536879578 peak = 1052676096
[M::mem_process_seqs] Processed 675688 reads in 211.885 CPU sec, 6.810 real sec
[M::mem_process_seqs] Processed 674918 reads in 231.418 CPU sec, 7.450 real sec
sorting block 87, #reads = 1065905 mem = 536883944 peak = 1052676096
[M::process] read 673836 sequences (100000224 bp) in 2.743 CPU sec, 2.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280372, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (360.77, 152.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673424 sequences (100000300 bp) in 2.141 CPU sec, 2.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280009, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (365.09, 155.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673986 reads in 186.416 CPU sec, 5.920 real sec
[M::mem_process_seqs] Processed 673836 reads in 117.070 CPU sec, 3.675 real sec
sorting block 88, #reads = 1066439 mem = 536878275 peak = 1052676096
[M::process] read 673200 sequences (100000189 bp) in 2.168 CPU sec, 2.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280107, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (367.74, 155.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672964 sequences (100000219 bp) in 2.438 CPU sec, 2.539 real sec...
[M::process] read 672600 sequences (100000287 bp) in 1.740 CPU sec, 1.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280258, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 358, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (371.46, 157.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673424 reads in 181.902 CPU sec, 5.772 real sec
sorting block 89, #reads = 1067653 mem = 536879593 peak = 1052676096
[M::mem_process_seqs] Processed 673200 reads in 116.497 CPU sec, 3.700 real sec
sorting block 90, #reads = 1067613 mem = 536879846 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280128, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 362, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (375.76, 160.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672580 sequences (100000048 bp) in 2.463 CPU sec, 2.578 real sec...
[M::process] read 672492 sequences (100000280 bp) in 1.930 CPU sec, 1.937 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279860, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 364, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (377.82, 160.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672964 reads in 175.609 CPU sec, 5.566 real sec
[M::mem_process_seqs] Processed 672600 reads in 112.214 CPU sec, 3.543 real sec
sorting block 91, #reads = 1069172 mem = 536881434 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279062, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 362, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (376.24, 159.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671982 sequences (100000066 bp) in 2.607 CPU sec, 2.678 real sec...
[M::process] read 671600 sequences (100000270 bp) in 1.820 CPU sec, 1.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279653, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 366, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (379.88, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672580 reads in 180.968 CPU sec, 5.734 real sec
[M::mem_process_seqs] Processed 672492 reads in 113.478 CPU sec, 3.596 real sec
sorting block 92, #reads = 1069956 mem = 536878263 peak = 1052676096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278813, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 373, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (386.96, 165.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671410 sequences (100000025 bp) in 2.316 CPU sec, 2.398 real sec...
[M::process] read 671406 sequences (100000205 bp) in 1.979 CPU sec, 1.980 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278808, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 380, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (394.64, 169.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671982 reads in 176.717 CPU sec, 5.602 real sec
[M::mem_process_seqs] Processed 671600 reads in 112.155 CPU sec, 3.549 real sec
sorting block 93, #reads = 1069085 mem = 536879020 peak = 1052676096
[M::process] read 671146 sequences (100000073 bp) in 2.275 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278408, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (397.72, 170.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671090 sequences (100000043 bp) in 1.510 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278343, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (395.77, 169.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671410 reads in 187.464 CPU sec, 5.967 real sec
sorting block 94, #reads = 1069402 mem = 536883015 peak = 1052676096
[M::mem_process_seqs] Processed 671406 reads in 120.532 CPU sec, 3.842 real sec
sorting block 95, #reads = 1069423 mem = 536879563 peak = 1061576704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278745, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (397.37, 169.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670886 sequences (100000244 bp) in 2.219 CPU sec, 2.316 real sec...
[M::process] read 670644 sequences (100000166 bp) in 1.510 CPU sec, 1.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278795, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (397.16, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671146 reads in 178.589 CPU sec, 5.680 real sec
[M::mem_process_seqs] Processed 671090 reads in 114.602 CPU sec, 3.655 real sec
sorting block 96, #reads = 1070904 mem = 536879041 peak = 1061576704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278380, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 387, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (401.56, 172.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670656 sequences (100000186 bp) in 2.396 CPU sec, 2.503 real sec...
[M::process] read 670466 sequences (100000089 bp) in 1.810 CPU sec, 1.811 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278012, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 389, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (404.00, 173.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670886 reads in 177.034 CPU sec, 5.617 real sec
[M::mem_process_seqs] Processed 670644 reads in 112.786 CPU sec, 3.575 real sec
sorting block 97, #reads = 1070576 mem = 536879279 peak = 1061576704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279023, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 394, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (409.29, 177.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673152 sequences (100000069 bp) in 2.494 CPU sec, 2.597 real sec...
[M::process] read 673816 sequences (100000194 bp) in 1.948 CPU sec, 1.959 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278737, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 395, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (411.47, 178.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1316)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670656 reads in 179.821 CPU sec, 5.699 real sec
[M::mem_process_seqs] Processed 670466 reads in 117.334 CPU sec, 3.716 real sec
sorting block 98, #reads = 1070283 mem = 536879353 peak = 1061576704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279185, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 358, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (378.52, 168.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673058 sequences (100000271 bp) in 2.506 CPU sec, 2.585 real sec...
[M::process] read 672632 sequences (100000081 bp) in 1.920 CPU sec, 1.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279528, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (368.40, 163.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673152 reads in 192.056 CPU sec, 6.097 real sec
sorting block 99, #reads = 1068918 mem = 536879432 peak = 1061576704
[M::process] read 672328 sequences (100000000 bp) in 1.945 CPU sec, 1.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279202, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 359, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (377.62, 165.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673058 reads in 91.914 CPU sec, 2.957 real sec
sorting block 100, #reads = 1066410 mem = 536879124 peak = 1098596352
[M::mem_process_seqs] Processed 673816 reads in 231.416 CPU sec, 7.401 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280054, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 362, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (379.54, 164.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672054 sequences (100000092 bp) in 2.312 CPU sec, 2.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279027, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 367, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (383.02, 166.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672108 sequences (100000289 bp) in 1.800 CPU sec, 1.802 real sec...
[M::mem_process_seqs] Processed 672632 reads in 186.727 CPU sec, 5.964 real sec
sorting block 101, #reads = 1072552 mem = 536879474 peak = 1098596352
[M::mem_process_seqs] Processed 672328 reads in 104.906 CPU sec, 3.317 real sec
sorting block 102, #reads = 1073525 mem = 536881203 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279508, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 368, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (384.45, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671814 sequences (100000266 bp) in 2.325 CPU sec, 2.436 real sec...
[M::process] read 671590 sequences (100000225 bp) in 1.710 CPU sec, 1.708 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278913, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 368, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (382.98, 164.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672054 reads in 162.729 CPU sec, 5.158 real sec
[M::mem_process_seqs] Processed 672108 reads in 103.350 CPU sec, 3.263 real sec
sorting block 103, #reads = 1073925 mem = 536878036 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279622, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 371, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (386.10, 165.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671594 sequences (100000274 bp) in 2.338 CPU sec, 2.432 real sec...
[M::process] read 671440 sequences (100000153 bp) in 1.760 CPU sec, 1.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278012, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 374, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (389.32, 166.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671814 reads in 162.816 CPU sec, 5.161 real sec
[M::mem_process_seqs] Processed 671590 reads in 101.874 CPU sec, 3.219 real sec
sorting block 104, #reads = 1072739 mem = 536876995 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278321, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 376, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (391.10, 167.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672180 sequences (100000167 bp) in 2.028 CPU sec, 2.129 real sec...
[M::process] read 671652 sequences (100000160 bp) in 1.707 CPU sec, 1.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278173, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (393.12, 167.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671594 reads in 161.334 CPU sec, 5.123 real sec
[M::mem_process_seqs] Processed 671440 reads in 101.069 CPU sec, 3.206 real sec
sorting block 105, #reads = 1072536 mem = 536879605 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278970, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 367, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (383.41, 165.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671692 sequences (100000119 bp) in 2.458 CPU sec, 2.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279098, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 374, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (389.45, 166.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671642 sequences (100000068 bp) in 1.890 CPU sec, 1.891 real sec...
[M::mem_process_seqs] Processed 672180 reads in 162.219 CPU sec, 5.131 real sec
sorting block 106, #reads = 1073683 mem = 536879551 peak = 1098596352
[M::mem_process_seqs] Processed 671652 reads in 101.417 CPU sec, 3.197 real sec
sorting block 107, #reads = 1072746 mem = 536884401 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278737, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 377, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (391.31, 168.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671088 sequences (100000109 bp) in 2.331 CPU sec, 2.413 real sec...
[M::process] read 670930 sequences (100000039 bp) in 1.630 CPU sec, 1.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279153, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (393.04, 168.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671692 reads in 166.039 CPU sec, 5.273 real sec
[M::mem_process_seqs] Processed 671642 reads in 102.310 CPU sec, 3.246 real sec
sorting block 108, #reads = 1070771 mem = 536879191 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278154, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (397.06, 170.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670958 sequences (100000098 bp) in 2.605 CPU sec, 2.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278149, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (400.67, 171.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670946 sequences (100000054 bp) in 1.860 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 671088 reads in 161.956 CPU sec, 5.128 real sec
[M::mem_process_seqs] Processed 670930 reads in 110.992 CPU sec, 3.515 real sec
sorting block 109, #reads = 1071392 mem = 536877661 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278868, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (398.88, 170.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671334 sequences (100000048 bp) in 2.409 CPU sec, 2.485 real sec...
[M::process] read 670960 sequences (100000041 bp) in 1.900 CPU sec, 1.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278435, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 385, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (400.45, 171.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670958 reads in 169.567 CPU sec, 5.384 real sec
[M::mem_process_seqs] Processed 670946 reads in 108.597 CPU sec, 3.489 real sec
sorting block 110, #reads = 1071409 mem = 536878635 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279236, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 381, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (397.61, 172.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670986 sequences (100000237 bp) in 2.205 CPU sec, 2.292 real sec...
[M::process] read 670854 sequences (100000204 bp) in 1.560 CPU sec, 1.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 278919, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 917, 2514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7286)
[M::mem_pestat] mean and std.dev: (1145.80, 1265.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9672)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (399.01, 171.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 671334 reads in 175.551 CPU sec, 5.632 real sec
sorting block 111, #reads = 1071810 mem = 536877474 peak = 1098596352
[M::mem_process_seqs] Processed 670960 reads in 108.617 CPU sec, 3.456 real sec
sorting block 112, #reads = 1071219 mem = 536877726 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278443, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (398.45, 170.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671052 sequences (100000113 bp) in 2.373 CPU sec, 2.439 real sec...
[M::process] read 670924 sequences (100000022 bp) in 1.940 CPU sec, 1.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278626, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (401.23, 171.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670986 reads in 172.519 CPU sec, 5.469 real sec
[M::mem_process_seqs] Processed 670854 reads in 110.768 CPU sec, 3.503 real sec
sorting block 113, #reads = 1071061 mem = 536880265 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279039, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 385, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (400.70, 172.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670846 sequences (100000075 bp) in 2.241 CPU sec, 2.320 real sec...
[M::process] read 675468 sequences (100000282 bp) in 1.610 CPU sec, 1.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278666, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 389, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (404.91, 175.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671052 reads in 171.380 CPU sec, 5.446 real sec
[M::mem_process_seqs] Processed 670924 reads in 106.215 CPU sec, 3.375 real sec
sorting block 114, #reads = 1070124 mem = 536882562 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278793, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 388, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (404.73, 176.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676246 sequences (100000129 bp) in 2.451 CPU sec, 2.510 real sec...
[M::process] read 674680 sequences (100000267 bp) in 1.950 CPU sec, 1.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279877, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 333, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (357.13, 162.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670846 reads in 188.459 CPU sec, 5.980 real sec
sorting block 115, #reads = 1067554 mem = 536878383 peak = 1098596352
[M::mem_process_seqs] Processed 675468 reads in 125.836 CPU sec, 3.991 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280884, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (348.60, 155.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673902 sequences (100000068 bp) in 2.425 CPU sec, 2.484 real sec...
[M::process] read 673274 sequences (100000200 bp) in 1.930 CPU sec, 1.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280833, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (360.05, 156.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676246 reads in 181.560 CPU sec, 5.751 real sec
sorting block 116, #reads = 1061230 mem = 536878397 peak = 1098596352
[M::mem_process_seqs] Processed 674680 reads in 113.737 CPU sec, 3.610 real sec
sorting block 117, #reads = 1064461 mem = 536878073 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280308, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (366.86, 158.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672898 sequences (100000238 bp) in 2.404 CPU sec, 2.465 real sec...
[M::process] read 672416 sequences (100000253 bp) in 1.929 CPU sec, 1.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280128, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 358, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (372.41, 159.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673902 reads in 174.599 CPU sec, 5.535 real sec
[M::mem_process_seqs] Processed 673274 reads in 109.997 CPU sec, 3.484 real sec
sorting block 118, #reads = 1066173 mem = 536878373 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280241, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 364, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (376.94, 160.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672294 sequences (100000212 bp) in 2.323 CPU sec, 2.388 real sec...
[M::process] read 671882 sequences (100000206 bp) in 1.780 CPU sec, 1.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279831, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 368, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (381.11, 161.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672898 reads in 167.241 CPU sec, 5.303 real sec
[M::mem_process_seqs] Processed 672416 reads in 103.612 CPU sec, 3.281 real sec
sorting block 119, #reads = 1067140 mem = 536879467 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279640, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 371, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (382.80, 161.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672142 sequences (100000125 bp) in 2.031 CPU sec, 2.118 real sec...
[M::process] read 671168 sequences (100000200 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279508, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (387.53, 163.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672294 reads in 163.531 CPU sec, 5.208 real sec
sorting block 120, #reads = 1066554 mem = 536881772 peak = 1098596352
[M::mem_process_seqs] Processed 671882 reads in 108.712 CPU sec, 3.488 real sec
sorting block 121, #reads = 1067647 mem = 536883532 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279131, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 369, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (384.16, 164.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671396 sequences (100000121 bp) in 2.003 CPU sec, 2.083 real sec...
[M::process] read 671336 sequences (100000150 bp) in 1.410 CPU sec, 1.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279034, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (394.86, 165.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672142 reads in 162.484 CPU sec, 5.171 real sec
[M::mem_process_seqs] Processed 671168 reads in 102.768 CPU sec, 3.270 real sec
sorting block 122, #reads = 1068162 mem = 536880853 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279137, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (395.27, 165.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671110 sequences (100000187 bp) in 2.050 CPU sec, 2.131 real sec...
[M::process] read 670934 sequences (100000068 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279355, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (392.52, 164.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671396 reads in 158.258 CPU sec, 5.032 real sec
[M::mem_process_seqs] Processed 671336 reads in 98.740 CPU sec, 3.136 real sec
sorting block 123, #reads = 1068064 mem = 536879466 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278562, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (391.16, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671030 sequences (100000133 bp) in 2.342 CPU sec, 2.396 real sec...
[M::process] read 671010 sequences (100000258 bp) in 1.790 CPU sec, 1.792 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278927, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (394.45, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671110 reads in 159.489 CPU sec, 5.283 real sec
[M::mem_process_seqs] Processed 670934 reads in 96.462 CPU sec, 3.052 real sec
sorting block 124, #reads = 1069552 mem = 536880574 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278916, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (396.76, 168.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670986 sequences (100000199 bp) in 2.025 CPU sec, 2.094 real sec...
[M::process] read 670862 sequences (100000129 bp) in 1.390 CPU sec, 1.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278396, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (394.01, 166.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671030 reads in 154.573 CPU sec, 4.918 real sec
sorting block 125, #reads = 1069495 mem = 536879772 peak = 1098596352
[M::mem_process_seqs] Processed 671010 reads in 95.494 CPU sec, 3.035 real sec
sorting block 126, #reads = 1070985 mem = 536880866 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278187, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (393.89, 166.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670640 sequences (100000232 bp) in 2.269 CPU sec, 2.313 real sec...
[M::process] read 670816 sequences (100000202 bp) in 1.520 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278249, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (397.05, 168.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670986 reads in 152.539 CPU sec, 4.837 real sec
[M::mem_process_seqs] Processed 670862 reads in 94.446 CPU sec, 2.988 real sec
sorting block 127, #reads = 1069624 mem = 536881848 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277392, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 386, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (401.02, 170.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670392 sequences (100000040 bp) in 2.337 CPU sec, 2.419 real sec...
[M::process] read 670602 sequences (100000167 bp) in 1.420 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278407, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 386, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (401.80, 172.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (429, 429, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (429, 429)
[M::mem_pestat] mean and std.dev: (429.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (429, 429)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 670640 reads in 147.332 CPU sec, 4.673 real sec
[M::mem_process_seqs] Processed 670816 reads in 92.400 CPU sec, 2.934 real sec
sorting block 128, #reads = 1070353 mem = 536878986 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277887, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 388, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (404.35, 173.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670516 sequences (100000175 bp) in 2.092 CPU sec, 2.164 real sec...
[M::process] read 91548 sequences (13614775 bp) in 0.420 CPU sec, 0.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278918, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 390, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (406.49, 174.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670392 reads in 144.375 CPU sec, 4.617 real sec
[M::mem_process_seqs] Processed 670602 reads in 89.323 CPU sec, 2.857 real sec
sorting block 129, #reads = 1071709 mem = 536878604 peak = 1098596352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 278283, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (945, 1005, 1005)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (825, 1125)
[M::mem_pestat] mean and std.dev: (978.78, 29.34)
[M::mem_pestat] low and high boundaries for proper pairs: (765, 1185)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 391, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (408.11, 176.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 37798, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 372, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (386.63, 167.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670516 reads in 86.813 CPU sec, 2.816 real sec
[M::mem_process_seqs] Processed 91548 reads in 24.598 CPU sec, 0.791 real sec
sorting block 130, #reads = 1072831 mem = 536879888 peak = 1156636672
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 140437636
overall: 70068936704 mem 20924.114 user 431.050 sys 699.449 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_Kh-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tLB:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 699.485 sec; CPU: 21355.170 sec
sorting block 131, #reads = 636271 mem = 319264626 peak = 1156636672
uniq header count 1
merging 132 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8326450383
output reads: 141119441
bam_mem_sort: 132 calls 71.196 user 1.712 sys 73.329 real
bam_write: 132 calls 37.920 user 2.507 sys 42.164 real
execute: 1 calls 37.276 user 1.423 sys 751.922 real
merge_files: 1 calls 37.250 user 1.396 sys 51.699 real
parse_chunk: 2106 calls 87.866 user 21.110 sys 111.091 real
read_chunk: 5673 calls 44.819 user 40.512 sys 643.576 real
sort_block: 1 calls 30.376 user 40.083 sys 621.865 real
write_chunk: 3565 calls 36.870 user 1.067 sys 50.717 real
overall: 3215941632 mem 2010.395 user 84.071 sys 751.943 real
ls: cannot access '*.cram': No such file or directory