cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2218.010 CPU sec, 91.929 real sec
[M::process] read 679018 sequences (100000147 bp) in 1.326 CPU sec, 1.334 real sec...
[M::process] read 677992 sequences (100000114 bp) in 1.515 CPU sec, 1.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281813, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (355.23, 162.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677406 sequences (100000177 bp) in 1.525 CPU sec, 1.547 real sec...
[M::process] read 676972 sequences (100000148 bp) in 1.520 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281625, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 349, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (362.40, 164.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679018 reads in 192.235 CPU sec, 6.332 real sec
[M::mem_process_seqs] Processed 677992 reads in 116.645 CPU sec, 3.814 real sec
sorting block 0, #reads = 1065819 mem = 536878811 peak = 595075072
[M::process] read 676720 sequences (100000068 bp) in 2.229 CPU sec, 2.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281822, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 355, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (366.95, 164.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676758 sequences (100000039 bp) in 1.717 CPU sec, 1.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281417, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 361, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (370.75, 165.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281030, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 365, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (374.03, 165.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677406 reads in 272.061 CPU sec, 9.254 real sec
[M::mem_process_seqs] Processed 676972 reads in 200.994 CPU sec, 6.961 real sec
[M::mem_process_seqs] Processed 676720 reads in 216.712 CPU sec, 7.513 real sec
sorting block 1, #reads = 1066581 mem = 536878047 peak = 722477056
[M::process] read 676618 sequences (100000229 bp) in 2.027 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280931, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 365, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (373.20, 164.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 317, 1252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3496)
[M::mem_pestat] mean and std.dev: (540.10, 476.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4618)
[M::mem_pestat] skip orientation RR
sorting block 2, #reads = 1065218 mem = 536878898 peak = 887222272
[M::process] read 676088 sequences (100000193 bp) in 2.134 CPU sec, 2.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281379, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 370, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (377.39, 166.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676758 reads in 189.740 CPU sec, 6.215 real sec
[M::process] read 675776 sequences (100000263 bp) in 1.785 CPU sec, 1.866 real sec...
[M::mem_process_seqs] Processed 676618 reads in 118.933 CPU sec, 3.853 real sec
sorting block 3, #reads = 1064473 mem = 536877991 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281453, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 373, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (380.24, 167.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675664 sequences (100000131 bp) in 2.183 CPU sec, 2.603 real sec...
[M::process] read 675460 sequences (100000163 bp) in 1.803 CPU sec, 1.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280805, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 373, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (380.41, 166.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676088 reads in 190.077 CPU sec, 6.315 real sec
[M::mem_process_seqs] Processed 675776 reads in 120.526 CPU sec, 4.011 real sec
sorting block 4, #reads = 1065941 mem = 536878862 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280287, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 374, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (381.79, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675394 sequences (100000182 bp) in 2.497 CPU sec, 2.786 real sec...
[M::process] read 675176 sequences (100000200 bp) in 1.713 CPU sec, 1.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281125, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 377, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (383.97, 167.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 320, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (327.10, 149.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675664 reads in 200.803 CPU sec, 6.760 real sec
[M::mem_process_seqs] Processed 675460 reads in 124.021 CPU sec, 4.124 real sec
sorting block 5, #reads = 1064826 mem = 536878058 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280706, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (383.51, 166.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 244, 879)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2281)
[M::mem_pestat] mean and std.dev: (422.90, 304.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2982)
[M::mem_pestat] skip orientation RR
[M::process] read 675622 sequences (100000212 bp) in 2.610 CPU sec, 3.084 real sec...
sorting block 6, #reads = 1065667 mem = 536879543 peak = 887222272
[M::process] read 675046 sequences (100000162 bp) in 1.811 CPU sec, 1.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279912, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 378, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (385.56, 167.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675394 reads in 200.818 CPU sec, 6.890 real sec
[M::mem_process_seqs] Processed 675176 reads in 124.670 CPU sec, 4.261 real sec
sorting block 7, #reads = 1064901 mem = 536878736 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280084, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 379, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (385.17, 167.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675082 sequences (100000218 bp) in 2.333 CPU sec, 2.568 real sec...
[M::process] read 674592 sequences (100000130 bp) in 1.796 CPU sec, 1.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279926, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 381, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (387.60, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675622 reads in 206.176 CPU sec, 6.951 real sec
[M::mem_process_seqs] Processed 675046 reads in 126.114 CPU sec, 4.212 real sec
sorting block 8, #reads = 1063891 mem = 536878033 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279842, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (387.08, 167.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674374 sequences (100000207 bp) in 2.722 CPU sec, 2.955 real sec...
[M::process] read 674676 sequences (100000301 bp) in 1.740 CPU sec, 1.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279279, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 380, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (386.32, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675082 reads in 213.874 CPU sec, 7.244 real sec
[M::mem_process_seqs] Processed 674592 reads in 131.944 CPU sec, 4.434 real sec
sorting block 9, #reads = 1063778 mem = 536879844 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280115, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 381, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (387.32, 165.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674414 sequences (100000007 bp) in 2.807 CPU sec, 3.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279926, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 376, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (383.19, 163.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674374 reads in 199.629 CPU sec, 6.711 real sec
[M::process] read 674302 sequences (100000211 bp) in 2.079 CPU sec, 2.151 real sec...
[M::mem_process_seqs] Processed 674676 reads in 121.666 CPU sec, 4.043 real sec
sorting block 10, #reads = 1063637 mem = 536878284 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279992, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 378, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (384.93, 163.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1064224 mem = 536883135 peak = 887222272
[M::process] read 674546 sequences (100000012 bp) in 3.251 CPU sec, 3.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280009, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 378, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (385.68, 164.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674414 reads in 199.807 CPU sec, 6.597 real sec
[M::process] read 674620 sequences (100000013 bp) in 1.902 CPU sec, 1.909 real sec...
[M::mem_process_seqs] Processed 674302 reads in 124.034 CPU sec, 4.058 real sec
sorting block 12, #reads = 1063608 mem = 536878571 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280457, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (387.11, 165.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674810 sequences (100000209 bp) in 2.847 CPU sec, 3.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279979, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 373, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (381.59, 163.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674722 sequences (100000049 bp) in 2.204 CPU sec, 2.227 real sec...
[M::mem_process_seqs] Processed 674546 reads in 203.866 CPU sec, 6.663 real sec
[M::mem_process_seqs] Processed 674620 reads in 126.740 CPU sec, 4.111 real sec
sorting block 13, #reads = 1061481 mem = 536883170 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280012, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 370, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (378.62, 162.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674638 sequences (100000162 bp) in 3.085 CPU sec, 3.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279853, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 371, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (379.82, 163.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 348, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (303.00, 68.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] skip orientation RR
[M::process] read 674252 sequences (100000225 bp) in 1.830 CPU sec, 1.887 real sec...
[M::mem_process_seqs] Processed 674810 reads in 211.901 CPU sec, 6.930 real sec
[M::mem_process_seqs] Processed 674722 reads in 130.265 CPU sec, 4.209 real sec
sorting block 14, #reads = 1062487 mem = 536880288 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280666, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 374, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (381.21, 163.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674414 sequences (100000031 bp) in 2.755 CPU sec, 3.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280238, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 382, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (389.57, 167.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674638 reads in 205.813 CPU sec, 6.782 real sec
[M::process] read 674288 sequences (100000112 bp) in 2.368 CPU sec, 2.385 real sec...
sorting block 15, #reads = 1061976 mem = 536878472 peak = 887222272
[M::mem_process_seqs] Processed 674252 reads in 129.990 CPU sec, 4.234 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280228, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 382, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (389.77, 168.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674336 sequences (100000074 bp) in 3.150 CPU sec, 3.647 real sec...
sorting block 16, #reads = 1061094 mem = 536880300 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280744, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 383, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (391.06, 169.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674414 reads in 203.770 CPU sec, 6.892 real sec
[M::mem_process_seqs] Processed 674288 reads in 127.878 CPU sec, 4.336 real sec
[M::process] read 678316 sequences (100000201 bp) in 2.266 CPU sec, 2.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280848, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 388, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (395.25, 172.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (407, 407, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (407, 407)
[M::mem_pestat] mean and std.dev: (407.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (407, 407)
[M::mem_pestat] skip orientation RR
sorting block 17, #reads = 1061577 mem = 536879772 peak = 887222272
[M::process] read 677502 sequences (100000112 bp) in 2.727 CPU sec, 3.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281107, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 349, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (363.53, 166.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674336 reads in 206.214 CPU sec, 6.808 real sec
[M::mem_process_seqs] Processed 678316 reads in 128.374 CPU sec, 4.235 real sec
[M::process] read 676840 sequences (100000157 bp) in 2.409 CPU sec, 2.771 real sec...
sorting block 18, #reads = 1061259 mem = 536878739 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281428, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 356, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (368.35, 167.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676618 sequences (100000057 bp) in 2.947 CPU sec, 3.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280244, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 363, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (373.80, 167.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677502 reads in 204.018 CPU sec, 6.826 real sec
[M::mem_process_seqs] Processed 676840 reads in 122.828 CPU sec, 4.085 real sec
sorting block 19, #reads = 1065753 mem = 536880390 peak = 887222272
[M::process] read 676478 sequences (100000245 bp) in 2.297 CPU sec, 2.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280525, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 376, 778)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970)
[M::mem_pestat] mean and std.dev: (334.00, 184.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2566)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 365, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (374.20, 166.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676230 sequences (100000239 bp) in 2.566 CPU sec, 2.950 real sec...
sorting block 20, #reads = 1067819 mem = 536881036 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280442, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 366, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (375.26, 166.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676618 reads in 199.394 CPU sec, 6.608 real sec
[M::mem_process_seqs] Processed 676478 reads in 122.062 CPU sec, 4.053 real sec
[M::process] read 676192 sequences (100000128 bp) in 2.406 CPU sec, 2.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280224, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 369, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (377.33, 167.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1066292 mem = 536883065 peak = 887222272
[M::process] read 675680 sequences (100000160 bp) in 2.953 CPU sec, 3.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281146, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 369, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (377.41, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676230 reads in 194.473 CPU sec, 6.568 real sec
[M::process] read 676034 sequences (100000284 bp) in 1.981 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280453, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 375, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (382.43, 168.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675680 reads in 108.847 CPU sec, 3.568 real sec
sorting block 22, #reads = 1066107 mem = 536877701 peak = 887222272
[M::process] read 675686 sequences (100000267 bp) in 2.272 CPU sec, 2.484 real sec...
[M::mem_process_seqs] Processed 676192 reads in 306.864 CPU sec, 10.341 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280708, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 375, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (382.56, 169.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 23, #reads = 1065528 mem = 536880177 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280431, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 372, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (380.32, 166.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675500 sequences (100000036 bp) in 3.022 CPU sec, 3.333 real sec...
[M::mem_process_seqs] Processed 676034 reads in 210.359 CPU sec, 6.930 real sec
[M::mem_process_seqs] Processed 675686 reads in 121.059 CPU sec, 4.021 real sec
[M::process] read 675506 sequences (100000160 bp) in 2.824 CPU sec, 3.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280480, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 372, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (380.37, 166.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1065839 mem = 536877447 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280640, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 375, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (382.95, 166.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675336 sequences (100000054 bp) in 2.557 CPU sec, 3.119 real sec...
[M::mem_process_seqs] Processed 675500 reads in 197.576 CPU sec, 6.554 real sec
[M::mem_process_seqs] Processed 675506 reads in 122.443 CPU sec, 4.080 real sec
[M::process] read 675224 sequences (100000020 bp) in 2.528 CPU sec, 2.959 real sec...
sorting block 25, #reads = 1065123 mem = 536878411 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281046, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 375, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (382.83, 166.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675224 sequences (100000194 bp) in 2.803 CPU sec, 3.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280234, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (384.85, 167.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 240, 964)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2412)
[M::mem_pestat] mean and std.dev: (400.62, 310.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3136)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675336 reads in 200.324 CPU sec, 6.564 real sec
[M::mem_process_seqs] Processed 675224 reads in 124.756 CPU sec, 4.028 real sec
sorting block 26, #reads = 1064888 mem = 536881903 peak = 887222272
[M::process] read 675108 sequences (100000083 bp) in 2.648 CPU sec, 3.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279760, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (385.09, 167.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280027, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (385.28, 167.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675024 sequences (100000188 bp) in 2.732 CPU sec, 3.190 real sec...
sorting block 27, #reads = 1064352 mem = 536880778 peak = 887222272
[M::mem_process_seqs] Processed 675224 reads in 198.763 CPU sec, 6.589 real sec
[M::mem_process_seqs] Processed 675108 reads in 123.994 CPU sec, 4.083 real sec
[M::process] read 675256 sequences (100000100 bp) in 2.495 CPU sec, 2.853 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280710, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 379, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (386.26, 167.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 28, #reads = 1064806 mem = 536880676 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280699, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 375, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (382.66, 165.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675106 sequences (100000035 bp) in 2.769 CPU sec, 3.327 real sec...
[M::mem_process_seqs] Processed 675024 reads in 201.048 CPU sec, 6.666 real sec
[M::mem_process_seqs] Processed 675256 reads in 127.143 CPU sec, 4.207 real sec
[M::process] read 674636 sequences (100000091 bp) in 2.562 CPU sec, 2.893 real sec...
sorting block 29, #reads = 1063609 mem = 536879345 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 281141, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (363, 372, 894)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1956)
[M::mem_pestat] mean and std.dev: (521.83, 252.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2487)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 377, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (384.51, 166.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674678 sequences (100000120 bp) in 2.681 CPU sec, 3.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280338, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 377, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (384.83, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675106 reads in 199.451 CPU sec, 6.605 real sec
[M::mem_process_seqs] Processed 674636 reads in 124.524 CPU sec, 4.148 real sec
[M::process] read 674928 sequences (100000202 bp) in 2.220 CPU sec, 2.437 real sec...
sorting block 30, #reads = 1063545 mem = 536875810 peak = 887222272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280314, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (384.45, 166.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675102 sequences (100000074 bp) in 2.899 CPU sec, 3.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280684, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 375, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (383.33, 166.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674678 reads in 200.561 CPU sec, 6.548 real sec
[M::mem_process_seqs] Processed 674928 reads in 126.513 CPU sec, 4.078 real sec
sorting block 31, #reads = 1063761 mem = 536879319 peak = 940183552
[M::process] read 674850 sequences (100000068 bp) in 2.712 CPU sec, 3.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280546, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 376, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (384.33, 167.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674900 sequences (100000289 bp) in 2.628 CPU sec, 3.061 real sec...
sorting block 32, #reads = 1063313 mem = 536879961 peak = 940183552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280711, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 374, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (383.52, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675102 reads in 196.320 CPU sec, 6.473 real sec
[M::mem_process_seqs] Processed 674850 reads in 123.640 CPU sec, 4.080 real sec
[M::process] read 674546 sequences (100000062 bp) in 2.200 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280169, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 371, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (380.63, 165.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 302, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (389.92, 197.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RR
sorting block 33, #reads = 1064063 mem = 536878280 peak = 940183552
[M::process] read 674780 sequences (100000289 bp) in 3.034 CPU sec, 3.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280575, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 375, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (384.43, 166.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674900 reads in 198.004 CPU sec, 6.528 real sec
[M::mem_process_seqs] Processed 674546 reads in 124.358 CPU sec, 4.066 real sec
[M::process] read 674556 sequences (100000196 bp) in 2.705 CPU sec, 2.955 real sec...
sorting block 34, #reads = 1063840 mem = 536878194 peak = 940183552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280549, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (384.67, 167.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674240 sequences (100000115 bp) in 2.715 CPU sec, 3.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280721, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 382, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (391.12, 170.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674780 reads in 194.909 CPU sec, 6.385 real sec
[M::mem_process_seqs] Processed 674556 reads in 121.260 CPU sec, 3.935 real sec
sorting block 35, #reads = 1063700 mem = 536881975 peak = 940183552
[M::process] read 674326 sequences (100000129 bp) in 2.641 CPU sec, 2.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280077, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (393.59, 171.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677984 sequences (100000136 bp) in 2.613 CPU sec, 2.914 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280883, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 385, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (393.24, 171.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 355, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (300.70, 76.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674240 reads in 191.890 CPU sec, 6.325 real sec
sorting block 36, #reads = 1063061 mem = 536877746 peak = 940183552
[M::mem_process_seqs] Processed 674326 reads in 120.311 CPU sec, 3.945 real sec
[M::process] read 678024 sequences (100000063 bp) in 3.064 CPU sec, 3.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282359, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 351, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (365.71, 168.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1062332 mem = 536879787 peak = 940183552
[M::process] read 676676 sequences (100000153 bp) in 2.597 CPU sec, 3.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282279, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 351, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (364.53, 167.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677984 reads in 191.308 CPU sec, 6.283 real sec
[M::mem_process_seqs] Processed 678024 reads in 118.173 CPU sec, 3.984 real sec
[M::process] read 676462 sequences (100000067 bp) in 2.480 CPU sec, 2.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281555, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 363, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (373.68, 167.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1065905 mem = 536877498 peak = 940183552
[M::process] read 676374 sequences (100000113 bp) in 2.992 CPU sec, 3.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281523, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 366, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (376.37, 167.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676676 reads in 190.656 CPU sec, 6.273 real sec
[M::mem_process_seqs] Processed 676462 reads in 117.325 CPU sec, 3.804 real sec
[M::process] read 676078 sequences (100000069 bp) in 2.795 CPU sec, 3.022 real sec...
sorting block 39, #reads = 1066775 mem = 536879333 peak = 941105152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281206, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 369, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (378.12, 167.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675636 sequences (100000224 bp) in 2.484 CPU sec, 2.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 281221, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 257, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (276.54, 81.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 373, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (381.69, 169.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676374 reads in 190.286 CPU sec, 6.255 real sec
[M::mem_process_seqs] Processed 676078 reads in 117.699 CPU sec, 3.841 real sec
sorting block 40, #reads = 1066236 mem = 536880217 peak = 941105152
[M::process] read 675584 sequences (100000117 bp) in 2.787 CPU sec, 2.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281073, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 376, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (384.58, 169.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1065159 mem = 536881164 peak = 941105152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280909, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (386.40, 170.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675472 sequences (100000144 bp) in 2.665 CPU sec, 2.783 real sec...
[M::mem_process_seqs] Processed 675636 reads in 168.067 CPU sec, 5.379 real sec
[M::mem_process_seqs] Processed 675584 reads in 103.113 CPU sec, 3.257 real sec
[M::process] read 675006 sequences (100000096 bp) in 2.653 CPU sec, 2.801 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280814, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (388.81, 172.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1065245 mem = 536881307 peak = 941105152
[M::process] read 675084 sequences (100000292 bp) in 2.737 CPU sec, 3.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280532, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (391.21, 172.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675472 reads in 174.579 CPU sec, 5.635 real sec
[M::mem_process_seqs] Processed 675006 reads in 112.304 CPU sec, 3.639 real sec
[M::process] read 675050 sequences (100000158 bp) in 2.373 CPU sec, 2.557 real sec...
sorting block 43, #reads = 1065900 mem = 536881510 peak = 941105152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279951, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (390.49, 172.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (318, 318, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (318, 318)
[M::mem_pestat] mean and std.dev: (318.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (318, 318)
[M::mem_pestat] skip orientation RR
[M::process] read 675164 sequences (100000241 bp) in 2.854 CPU sec, 3.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280686, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (392.09, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675084 reads in 188.320 CPU sec, 6.189 real sec
[M::mem_process_seqs] Processed 675050 reads in 116.223 CPU sec, 3.823 real sec
sorting block 44, #reads = 1064769 mem = 536877253 peak = 941105152
[M::process] read 675540 sequences (100000221 bp) in 3.023 CPU sec, 3.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281029, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (390.69, 171.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674738 sequences (100000148 bp) in 2.670 CPU sec, 2.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280946, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (386.32, 172.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675164 reads in 186.364 CPU sec, 6.117 real sec
[M::mem_process_seqs] Processed 675540 reads in 117.261 CPU sec, 3.872 real sec
sorting block 45, #reads = 1065857 mem = 536878874 peak = 941105152
[M::process] read 674612 sequences (100000194 bp) in 2.189 CPU sec, 2.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280492, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 385, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (393.31, 172.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1064488 mem = 536882526 peak = 972390400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280415, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (392.87, 171.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674540 sequences (100000173 bp) in 3.026 CPU sec, 3.517 real sec...
[M::mem_process_seqs] Processed 674738 reads in 190.649 CPU sec, 6.268 real sec
[M::mem_process_seqs] Processed 674612 reads in 121.131 CPU sec, 3.944 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280955, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 379, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (387.53, 168.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1063959 mem = 536889198 peak = 972390400
[M::process] read 674344 sequences (100000128 bp) in 3.141 CPU sec, 3.485 real sec...
[M::mem_process_seqs] Processed 674540 reads in 94.652 CPU sec, 3.131 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280854, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (369, 442, 1232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2958)
[M::mem_pestat] mean and std.dev: (627.10, 407.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3821)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (392.38, 170.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674352 sequences (100000053 bp) in 2.998 CPU sec, 3.189 real sec...
[M::mem_process_seqs] Processed 674344 reads in 97.611 CPU sec, 3.191 real sec
sorting block 48, #reads = 1064746 mem = 536882558 peak = 972390400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280563, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (390.57, 170.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674424 sequences (100000114 bp) in 2.680 CPU sec, 2.837 real sec...
[M::mem_process_seqs] Processed 674352 reads in 93.966 CPU sec, 3.033 real sec
sorting block 49, #reads = 1064513 mem = 536877630 peak = 972390400
[M::process] read 674526 sequences (100000151 bp) in 2.290 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280677, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (389.00, 170.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674942 sequences (100000092 bp) in 1.883 CPU sec, 1.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280429, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (389.82, 171.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 239, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (296.91, 143.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674424 reads in 174.761 CPU sec, 5.501 real sec
[M::process] read 674914 sequences (100000171 bp) in 1.767 CPU sec, 1.787 real sec...
[M::mem_process_seqs] Processed 674526 reads in 105.108 CPU sec, 3.302 real sec
sorting block 50, #reads = 1064763 mem = 536877115 peak = 972390400
[M::process] read 675068 sequences (100000220 bp) in 3.130 CPU sec, 3.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281036, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (388.41, 172.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674650 sequences (100000102 bp) in 1.459 CPU sec, 1.456 real sec...
[M::mem_process_seqs] Processed 674914 reads in 94.439 CPU sec, 2.980 real sec
sorting block 51, #reads = 1064103 mem = 536880680 peak = 972390400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280393, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (385.79, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280793, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (387.61, 172.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674468 sequences (100000197 bp) in 1.979 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 674942 reads in 271.890 CPU sec, 8.644 real sec
[M::mem_process_seqs] Processed 675068 reads in 123.096 CPU sec, 3.922 real sec
sorting block 52, #reads = 1063080 mem = 536880055 peak = 972390400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280903, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 379, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (388.38, 171.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674430 sequences (100000169 bp) in 2.464 CPU sec, 2.604 real sec...
[M::process] read 674300 sequences (100000243 bp) in 1.940 CPU sec, 1.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281212, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (389.58, 170.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674650 reads in 174.941 CPU sec, 5.556 real sec
[M::mem_process_seqs] Processed 674468 reads in 110.690 CPU sec, 3.507 real sec
sorting block 53, #reads = 1064370 mem = 536877744 peak = 972390400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281089, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 441, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (279.62, 126.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (390.54, 170.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674386 sequences (100000203 bp) in 2.450 CPU sec, 2.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280332, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (394.26, 173.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674494 sequences (100000031 bp) in 2.010 CPU sec, 2.007 real sec...
[M::mem_process_seqs] Processed 674430 reads in 172.635 CPU sec, 5.480 real sec
sorting block 54, #reads = 1062861 mem = 536878931 peak = 972390400
[M::mem_process_seqs] Processed 674300 reads in 108.262 CPU sec, 3.416 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281251, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 387, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (395.42, 173.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1063250 mem = 536879110 peak = 972390400
[M::process] read 678300 sequences (100000048 bp) in 2.598 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281735, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 390, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (397.29, 175.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678290 sequences (100000111 bp) in 1.965 CPU sec, 1.971 real sec...
[M::mem_process_seqs] Processed 674386 reads in 175.657 CPU sec, 5.581 real sec
[M::mem_process_seqs] Processed 674494 reads in 112.179 CPU sec, 3.540 real sec
sorting block 56, #reads = 1062935 mem = 536878200 peak = 972390400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281966, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 354, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (368.90, 171.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676440 sequences (100000032 bp) in 2.495 CPU sec, 2.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281450, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 350, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (366.17, 171.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675916 sequences (100000121 bp) in 1.950 CPU sec, 1.945 real sec...
[M::mem_process_seqs] Processed 678300 reads in 177.595 CPU sec, 5.642 real sec
[M::mem_process_seqs] Processed 678290 reads in 108.485 CPU sec, 3.424 real sec
sorting block 57, #reads = 1064096 mem = 536879838 peak = 972390400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280983, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 369, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (380.42, 172.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675836 sequences (100000136 bp) in 2.571 CPU sec, 2.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280739, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 373, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (383.40, 172.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675642 sequences (100000020 bp) in 1.940 CPU sec, 1.943 real sec...
[M::mem_process_seqs] Processed 676440 reads in 170.121 CPU sec, 5.396 real sec
[M::mem_process_seqs] Processed 675916 reads in 104.652 CPU sec, 3.297 real sec
sorting block 58, #reads = 1068495 mem = 536878041 peak = 972390400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280762, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 376, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (386.66, 173.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675412 sequences (100000204 bp) in 2.595 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280637, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (388.45, 173.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556)
[M::mem_pestat] mean and std.dev: (217.40, 79.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] skip orientation RR
[M::process] read 675052 sequences (100000046 bp) in 1.660 CPU sec, 1.653 real sec...
[M::mem_process_seqs] Processed 675836 reads in 165.664 CPU sec, 5.266 real sec
sorting block 59, #reads = 1066546 mem = 536881672 peak = 972390400
[M::mem_process_seqs] Processed 675642 reads in 101.987 CPU sec, 3.220 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280700, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (390.16, 173.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1066424 mem = 536879084 peak = 1001119744
[M::process] read 675072 sequences (100000084 bp) in 2.613 CPU sec, 2.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281373, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (393.03, 174.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675412 reads in 166.091 CPU sec, 5.276 real sec
[M::process] read 674980 sequences (100000153 bp) in 1.999 CPU sec, 2.009 real sec...
[M::mem_process_seqs] Processed 675052 reads in 104.857 CPU sec, 3.296 real sec
sorting block 61, #reads = 1066311 mem = 536878643 peak = 1001119744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280603, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 387, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (394.69, 174.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674594 sequences (100000092 bp) in 2.564 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280350, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (479, 479, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (479, 479)
[M::mem_pestat] mean and std.dev: (479.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (479, 479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 387, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (394.72, 174.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675072 reads in 167.226 CPU sec, 5.313 real sec
[M::process] read 674442 sequences (100000037 bp) in 1.993 CPU sec, 1.997 real sec...
[M::mem_process_seqs] Processed 674980 reads in 103.951 CPU sec, 3.270 real sec
sorting block 62, #reads = 1066572 mem = 536880283 peak = 1001119744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280705, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 388, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (395.27, 174.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674738 sequences (100000001 bp) in 2.702 CPU sec, 2.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280612, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (395.93, 173.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674594 reads in 169.046 CPU sec, 5.368 real sec
[M::process] read 675542 sequences (100000128 bp) in 2.029 CPU sec, 2.032 real sec...
[M::mem_process_seqs] Processed 674442 reads in 105.030 CPU sec, 3.302 real sec
sorting block 63, #reads = 1065503 mem = 536879956 peak = 1001119744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279713, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 386, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (393.25, 172.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 284, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (206, 336)
[M::mem_pestat] mean and std.dev: (275.33, 12.26)
[M::mem_pestat] low and high boundaries for proper pairs: (180, 362)
[M::mem_pestat] skip orientation RR
sorting block 64, #reads = 1064561 mem = 536877145 peak = 1001119744
[M::process] read 674558 sequences (100000080 bp) in 2.558 CPU sec, 2.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281423, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 377, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (386.94, 173.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674738 reads in 170.630 CPU sec, 5.421 real sec
[M::process] read 674200 sequences (100000247 bp) in 1.936 CPU sec, 1.944 real sec...
[M::mem_process_seqs] Processed 675542 reads in 106.421 CPU sec, 3.353 real sec
sorting block 65, #reads = 1065238 mem = 536876853 peak = 1001119744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279929, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 386, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (394.45, 172.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674344 sequences (100000201 bp) in 2.507 CPU sec, 2.628 real sec...
[M::process] read 674424 sequences (100000050 bp) in 1.510 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279867, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (396.33, 172.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674558 reads in 167.664 CPU sec, 5.339 real sec
[M::mem_process_seqs] Processed 674200 reads in 103.738 CPU sec, 3.293 real sec
sorting block 66, #reads = 1064198 mem = 536880991 peak = 1001119744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280014, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (390.66, 169.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674244 sequences (100000283 bp) in 2.378 CPU sec, 2.505 real sec...
[M::process] read 674540 sequences (100000057 bp) in 1.490 CPU sec, 1.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281005, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 379, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (388.19, 167.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674344 reads in 168.061 CPU sec, 5.355 real sec
[M::mem_process_seqs] Processed 674424 reads in 103.139 CPU sec, 3.273 real sec
sorting block 67, #reads = 1064340 mem = 536877280 peak = 1001119744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280489, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 378, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (386.98, 167.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674614 sequences (100000152 bp) in 2.653 CPU sec, 2.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280941, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 375, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (384.12, 166.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674244 reads in 169.129 CPU sec, 5.377 real sec
[M::process] read 674858 sequences (100000206 bp) in 1.963 CPU sec, 1.975 real sec...
sorting block 68, #reads = 1063403 mem = 536877910 peak = 1001119744
[M::mem_process_seqs] Processed 674540 reads in 104.610 CPU sec, 3.294 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280667, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 375, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (384.40, 167.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1063215 mem = 536880729 peak = 1001119744
[M::process] read 674572 sequences (100000195 bp) in 2.576 CPU sec, 2.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280824, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 377, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (385.98, 168.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674614 reads in 171.471 CPU sec, 5.445 real sec
[M::process] read 674546 sequences (100000301 bp) in 1.970 CPU sec, 1.965 real sec...
[M::mem_process_seqs] Processed 674858 reads in 108.103 CPU sec, 3.404 real sec
sorting block 70, #reads = 1063936 mem = 536877801 peak = 1001119744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280714, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (385.31, 167.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674776 sequences (100000244 bp) in 2.634 CPU sec, 2.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280533, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 378, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (387.24, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674572 reads in 166.860 CPU sec, 5.297 real sec
[M::process] read 674510 sequences (100000175 bp) in 2.127 CPU sec, 2.148 real sec...
[M::mem_process_seqs] Processed 674546 reads in 105.206 CPU sec, 3.302 real sec
sorting block 71, #reads = 1064240 mem = 536890029 peak = 1001119744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280790, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (387.28, 169.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674292 sequences (100000233 bp) in 2.240 CPU sec, 2.350 real sec...
[M::process] read 674286 sequences (100000184 bp) in 1.520 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280816, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (388.62, 169.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674776 reads in 167.054 CPU sec, 5.338 real sec
[M::mem_process_seqs] Processed 674510 reads in 103.212 CPU sec, 3.280 real sec
sorting block 72, #reads = 1063669 mem = 536881226 peak = 1001119744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281009, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (392.39, 170.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674060 sequences (100000277 bp) in 2.489 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281102, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 386, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (394.47, 171.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674042 sequences (100000065 bp) in 2.010 CPU sec, 2.005 real sec...
[M::mem_process_seqs] Processed 674292 reads in 169.244 CPU sec, 5.373 real sec
sorting block 73, #reads = 1064037 mem = 536883812 peak = 1001119744
[M::mem_process_seqs] Processed 674286 reads in 103.494 CPU sec, 3.263 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280737, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 388, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (396.30, 173.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1063574 mem = 536877038 peak = 1026220032
[M::process] read 677922 sequences (100000064 bp) in 2.644 CPU sec, 2.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281079, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 387, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (396.33, 172.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674060 reads in 167.324 CPU sec, 5.314 real sec
[M::process] read 680028 sequences (100000215 bp) in 2.219 CPU sec, 2.239 real sec...
[M::mem_process_seqs] Processed 674042 reads in 104.407 CPU sec, 3.281 real sec
sorting block 75, #reads = 1062655 mem = 536880962 peak = 1026220032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282745, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 355, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (370.35, 171.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678744 sequences (100000063 bp) in 2.715 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283388, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (355.56, 167.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678806 sequences (100000251 bp) in 2.020 CPU sec, 2.020 real sec...
[M::mem_process_seqs] Processed 677922 reads in 189.194 CPU sec, 6.016 real sec
[M::mem_process_seqs] Processed 680028 reads in 120.079 CPU sec, 3.792 real sec
sorting block 76, #reads = 1062219 mem = 536879027 peak = 1026220032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282283, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 351, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (365.04, 169.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677832 sequences (100000280 bp) in 2.606 CPU sec, 2.715 real sec...
[M::process] read 677258 sequences (100000071 bp) in 2.040 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281918, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (364.42, 167.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678744 reads in 187.500 CPU sec, 5.957 real sec
sorting block 77, #reads = 1062243 mem = 536878849 peak = 1026220032
[M::mem_process_seqs] Processed 678806 reads in 113.761 CPU sec, 3.591 real sec
sorting block 78, #reads = 1063697 mem = 536878352 peak = 1026220032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282506, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 358, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (367.64, 166.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676916 sequences (100000096 bp) in 2.592 CPU sec, 2.704 real sec...
[M::process] read 676636 sequences (100000028 bp) in 2.040 CPU sec, 2.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281169, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 366, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (374.17, 167.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677832 reads in 183.672 CPU sec, 5.826 real sec
[M::mem_process_seqs] Processed 677258 reads in 113.608 CPU sec, 3.583 real sec
sorting block 79, #reads = 1063332 mem = 536878647 peak = 1026220032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281744, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 370, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (377.89, 168.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676456 sequences (100000202 bp) in 2.577 CPU sec, 2.682 real sec...
[M::process] read 676312 sequences (100000155 bp) in 1.700 CPU sec, 1.701 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282242, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 370, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (377.73, 168.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676916 reads in 179.631 CPU sec, 5.717 real sec
[M::mem_process_seqs] Processed 676636 reads in 111.343 CPU sec, 3.528 real sec
sorting block 80, #reads = 1063833 mem = 536879429 peak = 1026220032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281404, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 372, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (380.43, 169.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676308 sequences (100000187 bp) in 2.108 CPU sec, 2.209 real sec...
[M::process] read 676514 sequences (100000255 bp) in 1.580 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281171, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (380.79, 168.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 228, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (144, 334)
[M::mem_pestat] mean and std.dev: (233.78, 18.65)
[M::mem_pestat] low and high boundaries for proper pairs: (106, 372)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676456 reads in 179.718 CPU sec, 5.735 real sec
[M::mem_process_seqs] Processed 676312 reads in 110.054 CPU sec, 3.504 real sec
sorting block 81, #reads = 1063418 mem = 536878555 peak = 1026220032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280638, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (380.19, 168.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676136 sequences (100000187 bp) in 2.576 CPU sec, 2.686 real sec...
[M::process] read 676514 sequences (100000143 bp) in 1.650 CPU sec, 1.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281773, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (379.35, 168.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676308 reads in 181.768 CPU sec, 5.788 real sec
sorting block 82, #reads = 1062873 mem = 536877718 peak = 1026220032
[M::mem_process_seqs] Processed 676514 reads in 111.362 CPU sec, 3.527 real sec
sorting block 83, #reads = 1062817 mem = 536878238 peak = 1026301952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281262, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (380.43, 167.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676232 sequences (100000117 bp) in 2.794 CPU sec, 2.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281212, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 372, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (379.13, 167.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676494 sequences (100000174 bp) in 2.060 CPU sec, 2.057 real sec...
[M::mem_process_seqs] Processed 676136 reads in 187.315 CPU sec, 5.946 real sec
[M::mem_process_seqs] Processed 676514 reads in 115.419 CPU sec, 3.645 real sec
sorting block 84, #reads = 1062133 mem = 536875934 peak = 1026301952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281304, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (380.26, 167.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676238 sequences (100000128 bp) in 2.445 CPU sec, 2.551 real sec...
[M::process] read 676316 sequences (100000110 bp) in 1.620 CPU sec, 1.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281470, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 372, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (379.40, 167.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676232 reads in 185.748 CPU sec, 5.920 real sec
[M::mem_process_seqs] Processed 676494 reads in 115.231 CPU sec, 3.659 real sec
sorting block 85, #reads = 1060331 mem = 536878362 peak = 1026301952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280856, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (381.77, 168.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675902 sequences (100000210 bp) in 2.203 CPU sec, 2.288 real sec...
[M::process] read 676018 sequences (100000066 bp) in 1.700 CPU sec, 1.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281425, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (381.77, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676238 reads in 183.041 CPU sec, 5.833 real sec
sorting block 86, #reads = 1061333 mem = 536879047 peak = 1026301952
[M::mem_process_seqs] Processed 676316 reads in 114.070 CPU sec, 3.623 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281271, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (380.52, 166.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675940 sequences (100000071 bp) in 2.521 CPU sec, 2.629 real sec...
[M::process] read 675660 sequences (100000283 bp) in 1.870 CPU sec, 1.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281237, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 372, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (379.34, 165.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675902 reads in 191.211 CPU sec, 6.084 real sec
sorting block 87, #reads = 1062242 mem = 536879848 peak = 1026301952
[M::mem_process_seqs] Processed 676018 reads in 118.785 CPU sec, 3.766 real sec
sorting block 88, #reads = 1060116 mem = 536878789 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281607, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 370, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (376.84, 163.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675682 sequences (100000049 bp) in 2.718 CPU sec, 2.812 real sec...
[M::process] read 675124 sequences (100000169 bp) in 2.040 CPU sec, 2.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280766, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 371, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (377.30, 162.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675940 reads in 190.807 CPU sec, 6.058 real sec
[M::mem_process_seqs] Processed 675660 reads in 118.709 CPU sec, 3.749 real sec
sorting block 89, #reads = 1060704 mem = 536878879 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281350, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 371, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (378.14, 163.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674784 sequences (100000273 bp) in 2.662 CPU sec, 2.756 real sec...
[M::process] read 675332 sequences (100000094 bp) in 2.080 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280945, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 376, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (382.12, 165.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675682 reads in 189.155 CPU sec, 6.002 real sec
[M::mem_process_seqs] Processed 675124 reads in 117.463 CPU sec, 3.704 real sec
sorting block 90, #reads = 1060832 mem = 536879350 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280838, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 379, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (385.75, 165.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674806 sequences (100000120 bp) in 2.631 CPU sec, 2.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281109, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 374, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (381.48, 165.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675082 sequences (100000228 bp) in 2.090 CPU sec, 2.083 real sec...
[M::mem_process_seqs] Processed 674784 reads in 185.711 CPU sec, 5.896 real sec
sorting block 91, #reads = 1060010 mem = 536877876 peak = 1093910528
[M::mem_process_seqs] Processed 675332 reads in 116.585 CPU sec, 3.680 real sec
sorting block 92, #reads = 1059304 mem = 536879310 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281574, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (388.96, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678612 sequences (100000066 bp) in 2.664 CPU sec, 2.758 real sec...
[M::process] read 684554 sequences (100000187 bp) in 1.960 CPU sec, 1.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281181, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (387.31, 169.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674806 reads in 185.677 CPU sec, 5.898 real sec
[M::mem_process_seqs] Processed 675082 reads in 117.478 CPU sec, 3.714 real sec
sorting block 93, #reads = 1060114 mem = 536877935 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282623, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 350, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (363.52, 167.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682724 sequences (100000285 bp) in 2.779 CPU sec, 2.864 real sec...
[M::process] read 681476 sequences (100000085 bp) in 2.170 CPU sec, 2.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285561, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 303, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (320.74, 150.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678612 reads in 202.039 CPU sec, 6.415 real sec
[M::mem_process_seqs] Processed 684554 reads in 132.932 CPU sec, 4.199 real sec
sorting block 94, #reads = 1058788 mem = 536879292 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284582, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (334.16, 156.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680916 sequences (100000224 bp) in 2.818 CPU sec, 2.909 real sec...
[M::process] read 679704 sequences (100000122 bp) in 2.130 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284566, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (341.03, 157.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682724 reads in 201.634 CPU sec, 6.402 real sec
[M::mem_process_seqs] Processed 681476 reads in 126.096 CPU sec, 3.989 real sec
sorting block 95, #reads = 1057136 mem = 536880847 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283871, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (344.69, 158.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679380 sequences (100000301 bp) in 2.370 CPU sec, 2.460 real sec...
[M::process] read 678500 sequences (100000274 bp) in 1.640 CPU sec, 1.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283404, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (351.27, 158.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680916 reads in 195.322 CPU sec, 6.229 real sec
sorting block 96, #reads = 1058501 mem = 536878110 peak = 1093910528
[M::mem_process_seqs] Processed 679704 reads in 121.652 CPU sec, 3.870 real sec
sorting block 97, #reads = 1057234 mem = 536878380 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283388, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (355.37, 160.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678314 sequences (100000168 bp) in 2.381 CPU sec, 2.471 real sec...
[M::process] read 677816 sequences (100000097 bp) in 2.100 CPU sec, 2.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283205, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 350, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (359.40, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679380 reads in 192.282 CPU sec, 6.114 real sec
[M::mem_process_seqs] Processed 678500 reads in 118.047 CPU sec, 3.750 real sec
sorting block 98, #reads = 1057805 mem = 536878728 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283237, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 353, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (361.23, 160.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677628 sequences (100000213 bp) in 2.773 CPU sec, 2.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282430, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 357, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (365.40, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677376 sequences (100000044 bp) in 2.070 CPU sec, 2.067 real sec...
[M::mem_process_seqs] Processed 678314 reads in 189.865 CPU sec, 6.028 real sec
[M::mem_process_seqs] Processed 677816 reads in 117.552 CPU sec, 3.713 real sec
sorting block 99, #reads = 1058974 mem = 536879196 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281977, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 360, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (368.13, 164.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677358 sequences (100000004 bp) in 2.698 CPU sec, 2.804 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282866, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 361, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (368.79, 163.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (362, 2116, 3887)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10937)
[M::mem_pestat] mean and std.dev: (1991.20, 1625.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14462)
[M::mem_pestat] skip orientation RR
[M::process] read 677152 sequences (100000123 bp) in 2.090 CPU sec, 2.093 real sec...
[M::mem_process_seqs] Processed 677628 reads in 187.875 CPU sec, 5.971 real sec
sorting block 100, #reads = 1059194 mem = 536881276 peak = 1093910528
[M::mem_process_seqs] Processed 677376 reads in 117.435 CPU sec, 3.707 real sec
sorting block 101, #reads = 1059871 mem = 536879085 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282587, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 367, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (270.33, 91.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 359, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (368.01, 163.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676522 sequences (100000061 bp) in 2.762 CPU sec, 2.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282521, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 363, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (371.23, 165.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676054 sequences (100000052 bp) in 2.100 CPU sec, 2.106 real sec...
[M::mem_process_seqs] Processed 677358 reads in 192.161 CPU sec, 6.101 real sec
[M::mem_process_seqs] Processed 677152 reads in 118.890 CPU sec, 3.752 real sec
sorting block 102, #reads = 1059506 mem = 536881961 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281907, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 369, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (377.48, 167.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676054 sequences (100000182 bp) in 2.855 CPU sec, 2.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281878, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 377, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (384.78, 171.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675750 sequences (100000210 bp) in 1.980 CPU sec, 1.980 real sec...
[M::mem_process_seqs] Processed 676522 reads in 188.635 CPU sec, 5.992 real sec
[M::mem_process_seqs] Processed 676054 reads in 117.922 CPU sec, 3.728 real sec
sorting block 103, #reads = 1060244 mem = 536884064 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281838, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (386.03, 172.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675634 sequences (100000250 bp) in 2.522 CPU sec, 2.622 real sec...
[M::process] read 675314 sequences (100000263 bp) in 1.620 CPU sec, 1.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281777, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (386.48, 171.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676054 reads in 193.306 CPU sec, 6.168 real sec
[M::mem_process_seqs] Processed 675750 reads in 121.325 CPU sec, 3.856 real sec
sorting block 104, #reads = 1060321 mem = 536879262 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281732, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (385.05, 170.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675092 sequences (100000252 bp) in 2.792 CPU sec, 2.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281237, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (386.82, 171.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674880 sequences (100000045 bp) in 2.074 CPU sec, 2.082 real sec...
[M::mem_process_seqs] Processed 675634 reads in 192.219 CPU sec, 6.104 real sec
sorting block 105, #reads = 1060434 mem = 536880029 peak = 1093910528
[M::mem_process_seqs] Processed 675314 reads in 119.177 CPU sec, 3.760 real sec
sorting block 106, #reads = 1060633 mem = 536877206 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281427, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (428, 429, 883)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1793)
[M::mem_pestat] mean and std.dev: (550.60, 256.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2248)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (390.28, 173.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675074 sequences (100000222 bp) in 2.555 CPU sec, 2.659 real sec...
[M::process] read 674594 sequences (100000189 bp) in 2.070 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281926, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (393.65, 174.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675092 reads in 187.370 CPU sec, 5.956 real sec
[M::mem_process_seqs] Processed 674880 reads in 116.114 CPU sec, 3.673 real sec
sorting block 107, #reads = 1060545 mem = 536879360 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281495, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 385, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (394.71, 176.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674652 sequences (100000258 bp) in 2.877 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281379, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 388, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (397.73, 177.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679234 sequences (100000071 bp) in 2.150 CPU sec, 2.157 real sec...
[M::mem_process_seqs] Processed 675074 reads in 186.476 CPU sec, 5.916 real sec
[M::mem_process_seqs] Processed 674594 reads in 117.316 CPU sec, 3.693 real sec
sorting block 108, #reads = 1059678 mem = 536878404 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281176, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 390, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (400.17, 179.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1323)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679326 sequences (100000178 bp) in 3.211 CPU sec, 3.276 real sec...
[M::process] read 677756 sequences (100000027 bp) in 2.030 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282985, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 344, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (359.71, 168.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674652 reads in 198.441 CPU sec, 6.293 real sec
sorting block 109, #reads = 1059516 mem = 536880808 peak = 1093910528
[M::mem_process_seqs] Processed 679234 reads in 128.598 CPU sec, 4.067 real sec
sorting block 110, #reads = 1053307 mem = 536879108 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282981, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (358.82, 167.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677498 sequences (100000033 bp) in 2.851 CPU sec, 2.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282597, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 359, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (369.92, 169.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677170 sequences (100000050 bp) in 1.930 CPU sec, 1.932 real sec...
[M::mem_process_seqs] Processed 679326 reads in 183.819 CPU sec, 5.834 real sec
[M::mem_process_seqs] Processed 677756 reads in 111.035 CPU sec, 3.506 real sec
sorting block 111, #reads = 1063684 mem = 536878849 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282328, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 361, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (371.85, 168.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676950 sequences (100000054 bp) in 3.312 CPU sec, 3.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282374, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 364, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (374.40, 169.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 403, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (338.80, 101.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677498 reads in 181.457 CPU sec, 5.750 real sec
[M::process] read 676526 sequences (100000108 bp) in 1.970 CPU sec, 1.978 real sec...
[M::mem_process_seqs] Processed 677170 reads in 112.638 CPU sec, 3.548 real sec
sorting block 112, #reads = 1063913 mem = 536877726 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282345, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 367, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (376.93, 170.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676528 sequences (100000179 bp) in 3.184 CPU sec, 3.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281869, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 368, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (376.58, 167.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676950 reads in 179.261 CPU sec, 5.679 real sec
[M::process] read 676108 sequences (100000161 bp) in 1.990 CPU sec, 1.990 real sec...
[M::mem_process_seqs] Processed 676526 reads in 112.631 CPU sec, 3.552 real sec
sorting block 113, #reads = 1063744 mem = 536879075 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282277, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 366, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (375.58, 167.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675844 sequences (100000247 bp) in 3.135 CPU sec, 3.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281432, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 370, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (380.02, 169.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675654 sequences (100000292 bp) in 1.920 CPU sec, 1.923 real sec...
[M::mem_process_seqs] Processed 676528 reads in 176.680 CPU sec, 5.599 real sec
sorting block 114, #reads = 1063877 mem = 536879503 peak = 1093910528
[M::mem_process_seqs] Processed 676108 reads in 110.081 CPU sec, 3.470 real sec
sorting block 115, #reads = 1063173 mem = 536878202 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281142, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (381.84, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675598 sequences (100000087 bp) in 3.093 CPU sec, 3.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281482, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 378, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (385.44, 170.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676712 sequences (100000164 bp) in 1.960 CPU sec, 1.959 real sec...
[M::mem_process_seqs] Processed 675844 reads in 178.328 CPU sec, 5.652 real sec
[M::mem_process_seqs] Processed 675654 reads in 111.197 CPU sec, 3.504 real sec
sorting block 116, #reads = 1063148 mem = 536880099 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281403, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (385.07, 170.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676202 sequences (100000169 bp) in 3.099 CPU sec, 3.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282001, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 364, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (374.72, 168.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675598 reads in 177.747 CPU sec, 5.641 real sec
[M::process] read 676064 sequences (100000030 bp) in 2.010 CPU sec, 2.008 real sec...
[M::mem_process_seqs] Processed 676712 reads in 109.749 CPU sec, 3.460 real sec
sorting block 117, #reads = 1063210 mem = 536878786 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281527, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 371, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (380.37, 170.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675936 sequences (100000252 bp) in 3.209 CPU sec, 3.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281796, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 373, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (382.14, 170.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675518 sequences (100000069 bp) in 1.990 CPU sec, 1.994 real sec...
[M::mem_process_seqs] Processed 676202 reads in 180.388 CPU sec, 5.711 real sec
sorting block 118, #reads = 1063705 mem = 536879756 peak = 1093910528
[M::mem_process_seqs] Processed 676064 reads in 111.855 CPU sec, 3.527 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281775, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 374, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (384.35, 171.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 119, #reads = 1062080 mem = 536881454 peak = 1093910528
[M::process] read 675266 sequences (100000249 bp) in 3.136 CPU sec, 3.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281096, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (385.15, 170.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675314 sequences (100000193 bp) in 1.973 CPU sec, 1.974 real sec...
[M::mem_process_seqs] Processed 675936 reads in 179.068 CPU sec, 5.673 real sec
[M::mem_process_seqs] Processed 675518 reads in 109.815 CPU sec, 3.464 real sec
sorting block 120, #reads = 1061540 mem = 536881718 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281550, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (390.31, 173.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675278 sequences (100000176 bp) in 2.853 CPU sec, 2.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281552, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 379, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (388.52, 172.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675276 sequences (100000128 bp) in 2.050 CPU sec, 2.045 real sec...
[M::mem_process_seqs] Processed 675266 reads in 180.412 CPU sec, 5.724 real sec
[M::mem_process_seqs] Processed 675314 reads in 112.701 CPU sec, 3.554 real sec
sorting block 121, #reads = 1062151 mem = 536876657 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281462, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (389.15, 172.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675850 sequences (100000121 bp) in 2.681 CPU sec, 2.723 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281415, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (389.28, 173.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674982 sequences (100000248 bp) in 2.010 CPU sec, 2.013 real sec...
[M::mem_process_seqs] Processed 675278 reads in 178.556 CPU sec, 5.669 real sec
[M::mem_process_seqs] Processed 675276 reads in 111.806 CPU sec, 3.533 real sec
sorting block 122, #reads = 1062186 mem = 536877989 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282240, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 374, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (384.61, 173.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674862 sequences (100000211 bp) in 2.827 CPU sec, 2.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280980, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (390.36, 172.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674988 sequences (100000192 bp) in 2.070 CPU sec, 2.067 real sec...
[M::mem_process_seqs] Processed 675850 reads in 181.279 CPU sec, 5.755 real sec
sorting block 123, #reads = 1061890 mem = 536879591 peak = 1093910528
[M::mem_process_seqs] Processed 674982 reads in 113.340 CPU sec, 3.579 real sec
sorting block 124, #reads = 1061038 mem = 536880365 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281342, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (389.01, 171.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675286 sequences (100000219 bp) in 2.915 CPU sec, 2.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281339, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (390.78, 174.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 209, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 375)
[M::mem_pestat] mean and std.dev: (203.82, 67.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RR
[M::process] read 675192 sequences (100000201 bp) in 2.090 CPU sec, 2.094 real sec...
[M::mem_process_seqs] Processed 674862 reads in 186.003 CPU sec, 5.900 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281251, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 379, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (389.59, 174.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675104 sequences (100000181 bp) in 2.624 CPU sec, 2.630 real sec...
[M::mem_process_seqs] Processed 675286 reads in 101.133 CPU sec, 3.188 real sec
sorting block 125, #reads = 1060695 mem = 536879306 peak = 1093910528
[M::mem_process_seqs] Processed 674988 reads in 241.712 CPU sec, 7.636 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281334, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (392.87, 176.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 126, #reads = 1058898 mem = 536877283 peak = 1093910528
[M::process] read 679580 sequences (100000299 bp) in 3.198 CPU sec, 3.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281260, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (394.58, 177.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675192 reads in 207.097 CPU sec, 6.546 real sec
[M::process] read 684554 sequences (100000000 bp) in 2.000 CPU sec, 1.998 real sec...
[M::mem_process_seqs] Processed 675104 reads in 118.318 CPU sec, 3.730 real sec
sorting block 127, #reads = 1058319 mem = 536879477 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282653, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (360.14, 170.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680956 sequences (100000239 bp) in 3.063 CPU sec, 3.205 real sec...
[M::process] read 679872 sequences (100000019 bp) in 2.090 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285538, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 309, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (328.60, 157.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679580 reads in 213.884 CPU sec, 6.797 real sec
[M::mem_process_seqs] Processed 684554 reads in 130.679 CPU sec, 4.144 real sec
sorting block 128, #reads = 1051738 mem = 536876902 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284167, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (348.18, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679056 sequences (100000048 bp) in 3.171 CPU sec, 3.284 real sec...
[M::process] read 677980 sequences (100000105 bp) in 2.060 CPU sec, 2.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283333, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 342, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.55, 161.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680956 reads in 202.265 CPU sec, 6.417 real sec
[M::mem_process_seqs] Processed 679872 reads in 122.296 CPU sec, 3.865 real sec
sorting block 129, #reads = 1057919 mem = 536877853 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283442, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (359.03, 162.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678086 sequences (100000200 bp) in 2.783 CPU sec, 2.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282796, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 358, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (366.12, 164.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 528, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208)
[M::mem_pestat] mean and std.dev: (377.17, 161.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1548)
[M::mem_pestat] skip orientation RR
[M::process] read 677256 sequences (100000096 bp) in 2.129 CPU sec, 2.132 real sec...
[M::mem_process_seqs] Processed 679056 reads in 193.048 CPU sec, 6.124 real sec
sorting block 130, #reads = 1056945 mem = 536880229 peak = 1093910528
[M::mem_process_seqs] Processed 677980 reads in 119.426 CPU sec, 3.768 real sec
sorting block 131, #reads = 1058066 mem = 536879551 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283119, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 358, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (366.76, 163.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676826 sequences (100000003 bp) in 3.224 CPU sec, 3.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282468, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 365, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (372.26, 165.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676488 sequences (100000040 bp) in 2.070 CPU sec, 2.072 real sec...
[M::mem_process_seqs] Processed 678086 reads in 193.840 CPU sec, 6.140 real sec
[M::mem_process_seqs] Processed 677256 reads in 120.670 CPU sec, 3.806 real sec
sorting block 132, #reads = 1056724 mem = 536879508 peak = 1093910528
[M::process] read 677150 sequences (100000210 bp) in 2.864 CPU sec, 2.987 real sec...
[M::process] read 675754 sequences (100000065 bp) in 2.090 CPU sec, 2.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282518, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 372, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (378.25, 166.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676488 reads in 104.239 CPU sec, 3.275 real sec
sorting block 133, #reads = 1057807 mem = 536880394 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282265, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 369, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (374.95, 164.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282838, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 270, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (271.10, 88.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 363, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (372.06, 166.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675694 sequences (100000102 bp) in 1.971 CPU sec, 1.981 real sec...
[M::mem_process_seqs] Processed 676826 reads in 290.457 CPU sec, 9.205 real sec
[M::mem_process_seqs] Processed 677150 reads in 130.272 CPU sec, 4.150 real sec
sorting block 134, #reads = 1058020 mem = 536878751 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282289, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 376, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (382.54, 167.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675822 sequences (100000270 bp) in 3.016 CPU sec, 3.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281687, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 379, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (384.35, 168.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675754 reads in 171.452 CPU sec, 5.437 real sec
[M::process] read 675352 sequences (100000125 bp) in 2.041 CPU sec, 2.057 real sec...
[M::mem_process_seqs] Processed 675694 reads in 107.210 CPU sec, 3.372 real sec
sorting block 135, #reads = 1059712 mem = 536879389 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281508, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (382.29, 167.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675560 sequences (100000229 bp) in 2.448 CPU sec, 2.565 real sec...
[M::process] read 675070 sequences (100000287 bp) in 2.100 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281545, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 375, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (382.00, 166.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675822 reads in 191.748 CPU sec, 6.099 real sec
[M::mem_process_seqs] Processed 675352 reads in 121.261 CPU sec, 3.846 real sec
sorting block 136, #reads = 1058992 mem = 536881267 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282076, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (381.50, 167.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 137, #reads = 1059180 mem = 536878837 peak = 1093910528
[M::process] read 675192 sequences (100000229 bp) in 2.794 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281594, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (387.67, 170.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674962 sequences (100000241 bp) in 2.030 CPU sec, 2.039 real sec...
[M::mem_process_seqs] Processed 675560 reads in 187.906 CPU sec, 5.973 real sec
[M::mem_process_seqs] Processed 675070 reads in 116.835 CPU sec, 3.690 real sec
sorting block 138, #reads = 1060108 mem = 536883529 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281471, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (384.54, 168.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675014 sequences (100000067 bp) in 3.039 CPU sec, 3.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281578, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (385.92, 169.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 334, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (277.00, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RR
[M::process] read 674458 sequences (100000264 bp) in 2.060 CPU sec, 2.060 real sec...
[M::mem_process_seqs] Processed 675192 reads in 187.052 CPU sec, 5.926 real sec
[M::mem_process_seqs] Processed 674962 reads in 115.762 CPU sec, 3.661 real sec
sorting block 139, #reads = 1060794 mem = 536878486 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281274, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (387.31, 169.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674444 sequences (100000253 bp) in 3.261 CPU sec, 3.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280768, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (390.21, 170.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674714 sequences (100000283 bp) in 2.113 CPU sec, 2.120 real sec...
[M::mem_process_seqs] Processed 675014 reads in 191.154 CPU sec, 6.060 real sec
[M::mem_process_seqs] Processed 674458 reads in 121.209 CPU sec, 3.824 real sec
sorting block 140, #reads = 1061021 mem = 536880405 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281335, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (393.41, 172.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674610 sequences (100000186 bp) in 2.668 CPU sec, 2.781 real sec...
[M::process] read 674190 sequences (100000166 bp) in 1.540 CPU sec, 1.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 281440, 5, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 217, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629)
[M::mem_pestat] mean and std.dev: (367.67, 249.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2113)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (392.39, 172.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674444 reads in 184.061 CPU sec, 5.864 real sec
sorting block 141, #reads = 1060553 mem = 536882347 peak = 1093910528
[M::mem_process_seqs] Processed 674714 reads in 113.809 CPU sec, 3.619 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280308, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (394.25, 174.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 142, #reads = 1061631 mem = 536884096 peak = 1093910528
[M::process] read 674266 sequences (100000291 bp) in 2.909 CPU sec, 3.055 real sec...
[M::process] read 208704 sequences (30856609 bp) in 0.910 CPU sec, 0.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280738, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (396.16, 175.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674610 reads in 180.794 CPU sec, 5.779 real sec
[M::mem_process_seqs] Processed 674190 reads in 112.901 CPU sec, 3.606 real sec
sorting block 143, #reads = 1061292 mem = 536880432 peak = 1093910528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280745, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 389, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (398.97, 177.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 87063, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 372, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (381.48, 172.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674266 reads in 122.012 CPU sec, 3.960 real sec
[M::mem_process_seqs] Processed 208704 reads in 47.528 CPU sec, 1.538 real sec
sorting block 144, #reads = 1060931 mem = 536877496 peak = 1093910528
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 153653376
overall: 69783572480 mem 24704.928 user 527.769 sys 843.363 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.L005\tPU:L005\tSM:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tLB:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R1.fastq.gz FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 843.399 sec; CPU: 25232.701 sec
sorting block 145, #reads = 272240 mem = 138157699 peak = 1093910528
uniq header count 1
merging 146 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 9078048587
output reads: 154344258
bam_mem_sort: 146 calls 90.050 user 1.438 sys 92.906 real
bam_write: 146 calls 58.575 user 3.631 sys 99.586 real
execute: 1 calls 42.675 user 1.185 sys 900.360 real
merge_files: 1 calls 42.649 user 1.162 sys 56.830 real
parse_chunk: 2325 calls 102.229 user 25.404 sys 143.698 real
read_chunk: 6273 calls 48.304 user 41.389 sys 765.837 real
sort_block: 1 calls 34.660 user 40.486 sys 743.898 real
write_chunk: 3946 calls 42.213 user 0.864 sys 55.855 real
overall: 3138203648 mem 2243.007 user 87.024 sys 900.380 real
ls: cannot access '*.cram': No such file or directory