cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_bgw-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2141.152 CPU sec, 79.357 real sec [M::process] read 694946 sequences (100000265 bp) in 1.334 CPU sec, 1.335 real sec... [M::process] read 693946 sequences (100000181 bp) in 1.720 CPU sec, 1.772 real sec... [M::process] read 692920 sequences (100000247 bp) in 1.696 CPU sec, 1.774 real sec... [M::process] read 692600 sequences (100000022 bp) in 1.598 CPU sec, 1.606 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 288337, 1, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 215, 2731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7807) [M::mem_pestat] mean and std.dev: (827.08, 1099.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 244, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (268.12, 128.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 259, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416) [M::mem_pestat] mean and std.dev: (298.90, 159.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693946 reads in 98.848 CPU sec, 3.156 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 288420, 4, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 509, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3997) [M::mem_pestat] mean and std.dev: (793.94, 678.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 240, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (263.50, 125.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 485, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2910) [M::mem_pestat] mean and std.dev: (551.20, 419.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287776, 6, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 249, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (272.53, 131.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::process] read 692514 sequences (100000118 bp) in 1.858 CPU sec, 1.868 real sec... [M::mem_process_seqs] Processed 694946 reads in 308.152 CPU sec, 9.966 real sec sorting block 0, #reads = 1087069 mem = 536877489 peak = 595202048 [M::mem_process_seqs] Processed 692920 reads in 215.766 CPU sec, 7.037 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 288340, 10, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 223, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2697) [M::mem_pestat] mean and std.dev: (425.09, 522.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 252, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (274.71, 131.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 907, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2526) [M::mem_pestat] mean and std.dev: (833.50, 596.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 329, 3743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10777) [M::mem_pestat] mean and std.dev: (1256.31, 1418.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692020 sequences (100000015 bp) in 2.348 CPU sec, 2.504 real sec... [M::process] read 692094 sequences (100000152 bp) in 1.772 CPU sec, 1.775 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287542, 4, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 253, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (276.61, 133.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 692600 reads in 170.068 CPU sec, 5.505 real sec sorting block 1, #reads = 1086803 mem = 536878653 peak = 774508544 [M::mem_process_seqs] Processed 692514 reads in 112.891 CPU sec, 3.702 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287943, 10, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 393, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300) [M::mem_pestat] mean and std.dev: (320.00, 117.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 254, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (277.96, 134.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (242, 400, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3185) [M::mem_pestat] mean and std.dev: (694.90, 618.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4166) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF sorting block 2, #reads = 1087797 mem = 536886063 peak = 949547008 [M::process] read 691314 sequences (100000287 bp) in 2.162 CPU sec, 2.479 real sec... [M::process] read 691212 sequences (100000195 bp) in 1.654 CPU sec, 1.713 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287640, 5, 18) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 254, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (278.79, 134.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 389, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (324.25, 162.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692020 reads in 171.392 CPU sec, 5.566 real sec [M::mem_process_seqs] Processed 692094 reads in 113.141 CPU sec, 3.638 real sec sorting block 3, #reads = 1086285 mem = 536878806 peak = 949547008 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 287517, 3, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 274, 918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2374) [M::mem_pestat] mean and std.dev: (409.27, 315.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 258, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (282.31, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 194, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 397) [M::mem_pestat] mean and std.dev: (206.93, 69.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690808 sequences (100000292 bp) in 2.474 CPU sec, 2.794 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287145, 9, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 259, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (282.71, 136.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (209.33, 58.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RR [M::process] read 690286 sequences (100000073 bp) in 1.806 CPU sec, 1.852 real sec... [M::mem_process_seqs] Processed 691314 reads in 171.465 CPU sec, 5.532 real sec [M::mem_process_seqs] Processed 691212 reads in 114.753 CPU sec, 3.707 real sec sorting block 4, #reads = 1086532 mem = 536883663 peak = 949547008 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287638, 6, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 276, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (301.67, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 261, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (284.28, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 689928 sequences (100000120 bp) in 2.084 CPU sec, 2.643 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287230, 3, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 262, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (285.03, 137.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 690808 reads in 169.358 CPU sec, 5.589 real sec [M::mem_process_seqs] Processed 690286 reads in 112.555 CPU sec, 3.635 real sec [M::process] read 689972 sequences (100000127 bp) in 1.965 CPU sec, 2.039 real sec... sorting block 5, #reads = 1086337 mem = 536878955 peak = 949547008 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287320, 24, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 191, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (307.62, 249.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 264, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (287.19, 138.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 65, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744) [M::mem_pestat] mean and std.dev: (244.61, 312.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2310) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF sorting block 6, #reads = 1086164 mem = 536877325 peak = 957366272 [M::process] read 689806 sequences (100000162 bp) in 2.498 CPU sec, 2.927 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287706, 1, 24) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 264, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (286.96, 138.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 349, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2619) [M::mem_pestat] mean and std.dev: (640.67, 544.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3417) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689928 reads in 170.766 CPU sec, 5.532 real sec [M::process] read 689610 sequences (100000263 bp) in 2.019 CPU sec, 2.049 real sec... [M::mem_process_seqs] Processed 689972 reads in 112.793 CPU sec, 3.617 real sec sorting block 7, #reads = 1086414 mem = 536881744 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287762, 6, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 265, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (288.24, 139.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 374, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (250.62, 101.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RR [M::process] read 689640 sequences (100000076 bp) in 2.346 CPU sec, 2.689 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 287117, 10, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 293, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1399) [M::mem_pestat] mean and std.dev: (371.64, 258.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 264, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (288.39, 139.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 216, 2229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6661) [M::mem_pestat] mean and std.dev: (771.90, 984.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8877) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 325, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2327) [M::mem_pestat] mean and std.dev: (338.78, 227.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689806 reads in 171.724 CPU sec, 5.532 real sec [M::process] read 689074 sequences (100000085 bp) in 1.930 CPU sec, 1.941 real sec... [M::mem_process_seqs] Processed 689610 reads in 114.725 CPU sec, 3.663 real sec sorting block 8, #reads = 1086452 mem = 536878773 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287392, 5, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 186, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.71, 80.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 265, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (288.60, 139.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 166, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (137, 197) [M::mem_pestat] mean and std.dev: (168.13, 4.29) [M::mem_pestat] low and high boundaries for proper pairs: (125, 209) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688776 sequences (100000148 bp) in 2.710 CPU sec, 3.023 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286830, 1, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 267, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (289.24, 138.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 266, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (325.92, 195.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1569) [M::mem_pestat] skip orientation RR [M::process] read 688356 sequences (100000143 bp) in 1.981 CPU sec, 2.001 real sec... [M::mem_process_seqs] Processed 689640 reads in 175.384 CPU sec, 5.605 real sec [M::mem_process_seqs] Processed 689074 reads in 115.306 CPU sec, 3.681 real sec sorting block 9, #reads = 1084909 mem = 536879237 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286971, 9, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 650, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2466) [M::mem_pestat] mean and std.dev: (481.90, 363.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 267, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (289.53, 138.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 235, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 489) [M::mem_pestat] mean and std.dev: (229.47, 69.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687852 sequences (100000004 bp) in 2.508 CPU sec, 2.791 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287025, 4, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 210, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (258.10, 98.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 269, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (290.66, 138.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 216, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (263.62, 81.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688776 reads in 172.612 CPU sec, 5.526 real sec [M::process] read 687722 sequences (100000249 bp) in 1.866 CPU sec, 1.877 real sec... sorting block 10, #reads = 1084540 mem = 536883042 peak = 957366272 [M::mem_process_seqs] Processed 688356 reads in 113.300 CPU sec, 3.598 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287077, 4, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 147, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283) [M::mem_pestat] mean and std.dev: (253.73, 183.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 269, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (289.56, 136.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF sorting block 11, #reads = 1084932 mem = 536876477 peak = 957366272 [M::process] read 687750 sequences (100000239 bp) in 2.886 CPU sec, 3.220 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 286975, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 245, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (257.19, 104.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 270, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (290.78, 137.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 687852 reads in 171.444 CPU sec, 5.505 real sec [M::process] read 687778 sequences (100000096 bp) in 2.160 CPU sec, 2.238 real sec... [M::mem_process_seqs] Processed 687722 reads in 113.813 CPU sec, 3.621 real sec sorting block 12, #reads = 1085411 mem = 536885744 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 286964, 5, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (436, 1214, 2187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5689) [M::mem_pestat] mean and std.dev: (1479.08, 1382.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 269, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (290.01, 137.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 174, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (115, 225) [M::mem_pestat] mean and std.dev: (164.27, 21.75) [M::mem_pestat] low and high boundaries for proper pairs: (77, 251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688188 sequences (100000296 bp) in 2.365 CPU sec, 2.599 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286588, 11, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 337, 2830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8142) [M::mem_pestat] mean and std.dev: (892.25, 1123.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 268, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (289.39, 136.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (7, 591, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1759) [M::mem_pestat] mean and std.dev: (325.55, 290.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 247, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1673) [M::mem_pestat] mean and std.dev: (415.27, 340.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687750 reads in 176.134 CPU sec, 5.682 real sec [M::process] read 687788 sequences (100000224 bp) in 2.017 CPU sec, 2.120 real sec... [M::mem_process_seqs] Processed 687778 reads in 117.864 CPU sec, 3.757 real sec sorting block 13, #reads = 1083582 mem = 536889145 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286656, 4, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 418, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (289.75, 132.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 265, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (285.90, 133.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (99, 259, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2511) [M::mem_pestat] mean and std.dev: (397.50, 349.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687926 sequences (100000199 bp) in 2.782 CPU sec, 3.416 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 286694, 8, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 341, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (348.16, 127.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 265, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (285.79, 133.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 688188 reads in 177.021 CPU sec, 5.712 real sec [M::mem_process_seqs] Processed 687788 reads in 116.052 CPU sec, 3.718 real sec [M::process] read 687618 sequences (100000082 bp) in 2.200 CPU sec, 2.391 real sec... sorting block 14, #reads = 1082152 mem = 536885590 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286677, 4, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 265, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (286.17, 134.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 167, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 290) [M::mem_pestat] mean and std.dev: (157.44, 51.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RR [M::process] read 687864 sequences (100000215 bp) in 2.653 CPU sec, 2.947 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 286302, 5, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 253, 2512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7166) [M::mem_pestat] mean and std.dev: (1094.28, 1131.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 269, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (291.19, 138.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 403, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (332.36, 191.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687926 reads in 182.224 CPU sec, 5.936 real sec sorting block 15, #reads = 1083019 mem = 536877663 peak = 957366272 [M::mem_process_seqs] Processed 687618 reads in 121.241 CPU sec, 3.900 real sec [M::process] read 687796 sequences (100000179 bp) in 2.145 CPU sec, 2.237 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286388, 6, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 271, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (331.31, 179.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 267, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (290.01, 138.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 178, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (187.55, 32.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 16, #reads = 1081260 mem = 536881985 peak = 957366272 [M::process] read 687874 sequences (100000177 bp) in 2.880 CPU sec, 3.420 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286609, 6, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 196, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 364) [M::mem_pestat] mean and std.dev: (209.36, 63.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 268, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (290.45, 138.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (345, 539, 3436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9618) [M::mem_pestat] mean and std.dev: (2301.27, 3419.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687864 reads in 180.560 CPU sec, 5.940 real sec [M::mem_process_seqs] Processed 687796 reads in 119.857 CPU sec, 3.968 real sec [M::process] read 693790 sequences (100000143 bp) in 2.267 CPU sec, 2.389 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286274, 6, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 972, 2683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7567) [M::mem_pestat] mean and std.dev: (1257.14, 1040.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 268, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (292.01, 140.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (110, 182, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (186.15, 74.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 17, #reads = 1080634 mem = 536880193 peak = 957366272 [M::process] read 693626 sequences (100000151 bp) in 2.595 CPU sec, 3.222 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 288507, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 294, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1492) [M::mem_pestat] mean and std.dev: (306.05, 188.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 246, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (270.00, 130.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 232, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (356.96, 327.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687874 reads in 178.594 CPU sec, 5.898 real sec [M::mem_process_seqs] Processed 693790 reads in 118.565 CPU sec, 3.876 real sec [M::process] read 692724 sequences (100000094 bp) in 2.519 CPU sec, 2.816 real sec... sorting block 18, #reads = 1081464 mem = 536876635 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288504, 8, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 651, 1355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3677) [M::mem_pestat] mean and std.dev: (476.62, 383.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 245, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (270.05, 130.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 243, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (234.11, 111.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692130 sequences (100000216 bp) in 2.578 CPU sec, 3.049 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287868, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 214, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (174.88, 64.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 250, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (274.30, 132.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 230, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 437) [M::mem_pestat] mean and std.dev: (213.21, 63.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693626 reads in 172.185 CPU sec, 5.639 real sec [M::mem_process_seqs] Processed 692724 reads in 114.387 CPU sec, 3.683 real sec sorting block 19, #reads = 1085361 mem = 536879683 peak = 957366272 [M::process] read 692014 sequences (100000229 bp) in 2.464 CPU sec, 2.713 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 287862, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 469, 4200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12118) [M::mem_pestat] mean and std.dev: (1817.82, 2024.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 252, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (275.72, 132.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 4303, 4303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12373) [M::mem_pestat] mean and std.dev: (3221.56, 2769.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 20, #reads = 1088286 mem = 536880653 peak = 957366272 [M::process] read 691914 sequences (100000077 bp) in 2.311 CPU sec, 2.739 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 288314, 4, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 240, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1921) [M::mem_pestat] mean and std.dev: (376.56, 288.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 253, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (276.61, 133.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 356, 1944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5358) [M::mem_pestat] mean and std.dev: (858.65, 806.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692130 reads in 170.818 CPU sec, 5.615 real sec [M::mem_process_seqs] Processed 692014 reads in 113.635 CPU sec, 3.771 real sec [M::process] read 691722 sequences (100000135 bp) in 2.240 CPU sec, 2.407 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 287771, 8, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 440, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (315.37, 168.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 254, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (277.61, 133.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (345, 464, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (425.65, 166.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 21, #reads = 1087582 mem = 536882032 peak = 957366272 [M::process] read 691622 sequences (100000298 bp) in 2.610 CPU sec, 3.279 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287655, 7, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 499, 1370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3584) [M::mem_pestat] mean and std.dev: (923.29, 1037.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 254, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (277.92, 133.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 691914 reads in 170.160 CPU sec, 5.586 real sec [M::mem_process_seqs] Processed 691722 reads in 112.972 CPU sec, 3.695 real sec [M::process] read 690734 sequences (100000018 bp) in 2.540 CPU sec, 2.751 real sec... sorting block 22, #reads = 1087348 mem = 536880113 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 288282, 8, 15) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 256, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (279.91, 135.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 408, 716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1788) [M::mem_pestat] mean and std.dev: (517.87, 362.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2324) [M::mem_pestat] skip orientation RR [M::process] read 691184 sequences (100000076 bp) in 2.185 CPU sec, 2.957 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287616, 5, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 208, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1696) [M::mem_pestat] mean and std.dev: (393.20, 406.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 260, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (284.03, 138.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 691622 reads in 169.948 CPU sec, 5.597 real sec [M::mem_process_seqs] Processed 690734 reads in 111.843 CPU sec, 3.659 real sec sorting block 23, #reads = 1087127 mem = 536879368 peak = 957366272 [M::process] read 690802 sequences (100000194 bp) in 2.598 CPU sec, 2.768 real sec... [M::process] read 690230 sequences (100000087 bp) in 2.829 CPU sec, 3.202 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287690, 1, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 260, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (282.33, 135.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (98, 287, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (229.40, 160.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690802 reads in 95.960 CPU sec, 3.051 real sec sorting block 24, #reads = 1086831 mem = 536877447 peak = 957366272 [M::process] read 690166 sequences (100000030 bp) in 2.436 CPU sec, 2.640 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 287415, 6, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 224, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 353) [M::mem_pestat] mean and std.dev: (191.06, 48.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (281.65, 136.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287272, 2, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 363, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029) [M::mem_pestat] mean and std.dev: (582.00, 601.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (283.27, 136.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 348, 1103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (550.36, 482.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691184 reads in 273.314 CPU sec, 8.796 real sec [M::mem_process_seqs] Processed 690230 reads in 138.034 CPU sec, 4.436 real sec sorting block 25, #reads = 1086471 mem = 536880909 peak = 957366272 [M::process] read 689670 sequences (100000268 bp) in 2.966 CPU sec, 3.171 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 287788, 2, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 238, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272) [M::mem_pestat] mean and std.dev: (321.53, 164.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 262, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (284.74, 136.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 470, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (354.80, 133.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689520 sequences (100000290 bp) in 2.460 CPU sec, 2.777 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287223, 11, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 263, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (285.82, 136.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 348, 1404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3864) [M::mem_pestat] mean and std.dev: (684.55, 764.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5094) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 491, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (427.08, 137.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690166 reads in 177.699 CPU sec, 5.777 real sec [M::mem_process_seqs] Processed 689670 reads in 119.216 CPU sec, 3.822 real sec sorting block 26, #reads = 1086468 mem = 536882476 peak = 957366272 [M::process] read 689346 sequences (100000140 bp) in 2.796 CPU sec, 3.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287542, 10, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 264, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (287.55, 138.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 662, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1834) [M::mem_pestat] mean and std.dev: (359.89, 273.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2420) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 202, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (175.78, 46.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 27, #reads = 1086594 mem = 536883990 peak = 957366272 [M::process] read 689468 sequences (100000220 bp) in 2.244 CPU sec, 2.478 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286984, 5, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 264, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (287.69, 138.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (338, 539, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3137) [M::mem_pestat] mean and std.dev: (684.58, 458.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4070) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689520 reads in 176.613 CPU sec, 5.748 real sec [M::process] read 689436 sequences (100000266 bp) in 1.912 CPU sec, 2.029 real sec... [M::mem_process_seqs] Processed 689346 reads in 118.603 CPU sec, 3.946 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287415, 11, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 436, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2956) [M::mem_pestat] mean and std.dev: (587.27, 402.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 264, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (287.49, 138.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (363, 363, 1736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4482) [M::mem_pestat] mean and std.dev: (592.70, 577.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 248, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (227.89, 103.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 28, #reads = 1085875 mem = 536878601 peak = 957366272 [M::process] read 689058 sequences (100000193 bp) in 2.672 CPU sec, 3.214 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287263, 5, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 432, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2095) [M::mem_pestat] mean and std.dev: (657.67, 664.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 264, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (287.96, 139.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 426, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (647.21, 671.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689468 reads in 178.040 CPU sec, 5.818 real sec [M::process] read 688782 sequences (100000011 bp) in 2.337 CPU sec, 2.675 real sec... sorting block 29, #reads = 1086424 mem = 536880095 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 287174, 5, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 216, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (218.81, 53.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 265, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (287.80, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 469, 8170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23826) [M::mem_pestat] mean and std.dev: (2326.92, 3374.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 31654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689058 reads in 100.486 CPU sec, 3.292 real sec [M::process] read 688400 sequences (100000000 bp) in 2.453 CPU sec, 2.753 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286778, 8, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 266, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (288.77, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 207, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (238.67, 94.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689436 reads in 299.924 CPU sec, 9.881 real sec [M::mem_process_seqs] Processed 688782 reads in 116.896 CPU sec, 3.985 real sec sorting block 30, #reads = 1084739 mem = 536882228 peak = 957366272 [M::process] read 688264 sequences (100000011 bp) in 2.625 CPU sec, 2.928 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 286899, 9, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 238, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (217.88, 77.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (287.48, 136.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 688558 sequences (100000298 bp) in 2.535 CPU sec, 2.865 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286628, 7, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (287.54, 136.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 688400 reads in 181.540 CPU sec, 6.038 real sec sorting block 31, #reads = 1083887 mem = 536881213 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286773, 3, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 338, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3332) [M::mem_pestat] mean and std.dev: (578.29, 531.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 264, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (286.10, 135.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 238, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 491) [M::mem_pestat] mean and std.dev: (229.55, 39.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688794 sequences (100000070 bp) in 3.001 CPU sec, 3.587 real sec... [M::mem_process_seqs] Processed 688558 reads in 102.931 CPU sec, 3.658 real sec sorting block 32, #reads = 1084469 mem = 536880755 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 286972, 10, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 468, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595) [M::mem_pestat] mean and std.dev: (453.12, 260.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 263, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (285.62, 136.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (569, 765, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (177, 1157) [M::mem_pestat] mean and std.dev: (619.71, 182.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 406, 2011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5637) [M::mem_pestat] mean and std.dev: (1003.67, 1071.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688642 sequences (100000031 bp) in 2.299 CPU sec, 3.134 real sec... [M::mem_process_seqs] Processed 688264 reads in 316.436 CPU sec, 10.952 real sec [M::mem_process_seqs] Processed 688794 reads in 98.240 CPU sec, 3.421 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287199, 5, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 344, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (338.08, 150.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 264, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (285.46, 135.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 266, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (235.87, 135.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688010 sequences (100000088 bp) in 2.781 CPU sec, 3.105 real sec... [M::mem_process_seqs] Processed 688642 reads in 102.614 CPU sec, 3.520 real sec sorting block 33, #reads = 1083909 mem = 536882422 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286895, 13, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 286, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (259.15, 125.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (286.47, 134.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (372, 372, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (204, 624) [M::mem_pestat] mean and std.dev: (418.67, 41.74) [M::mem_pestat] low and high boundaries for proper pairs: (120, 708) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 687864 sequences (100000123 bp) in 2.462 CPU sec, 3.081 real sec... [M::mem_process_seqs] Processed 688010 reads in 100.179 CPU sec, 3.443 real sec sorting block 34, #reads = 1084560 mem = 536878222 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286361, 5, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 350, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 683) [M::mem_pestat] mean and std.dev: (301.50, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 265, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (287.12, 135.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 269, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (271.23, 83.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687928 sequences (100000183 bp) in 2.956 CPU sec, 3.195 real sec... [M::mem_process_seqs] Processed 687864 reads in 100.706 CPU sec, 3.393 real sec [M::process] read 687920 sequences (100000057 bp) in 2.690 CPU sec, 3.114 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286478, 12, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 310, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268) [M::mem_pestat] mean and std.dev: (331.00, 190.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 266, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (288.80, 137.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 1290, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3330) [M::mem_pestat] mean and std.dev: (1015.00, 648.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 460, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1866) [M::mem_pestat] mean and std.dev: (378.12, 241.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 35, #reads = 1083109 mem = 536878289 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 286849, 5, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 229, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (233.33, 120.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 268, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (291.13, 139.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 252, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 464) [M::mem_pestat] mean and std.dev: (254.84, 89.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687906 sequences (100000181 bp) in 2.523 CPU sec, 2.963 real sec... [M::mem_process_seqs] Processed 687928 reads in 182.375 CPU sec, 6.169 real sec [M::mem_process_seqs] Processed 687920 reads in 119.888 CPU sec, 4.053 real sec [M::process] read 691376 sequences (100000137 bp) in 2.411 CPU sec, 2.843 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286537, 18, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 289, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (231.11, 157.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 269, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (291.48, 139.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 139, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (239.56, 156.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 399, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (405.18, 282.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 36, #reads = 1083475 mem = 536882590 peak = 957366272 [M::mem_process_seqs] Processed 687906 reads in 101.431 CPU sec, 3.528 real sec [M::process] read 694308 sequences (100000127 bp) in 2.702 CPU sec, 2.986 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 288024, 4, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 590, 4776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13998) [M::mem_pestat] mean and std.dev: (1658.26, 1949.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 253, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (276.84, 133.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 245, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (285.45, 128.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 37, #reads = 1083178 mem = 536880983 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 288512, 3, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 225, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (221.25, 86.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 243, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (265.84, 127.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 185, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1465) [M::mem_pestat] mean and std.dev: (216.55, 136.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693202 sequences (100000047 bp) in 3.085 CPU sec, 3.487 real sec... [M::mem_process_seqs] Processed 691376 reads in 186.094 CPU sec, 6.113 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 288635, 5, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 349, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2305) [M::mem_pestat] mean and std.dev: (608.24, 620.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 248, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (271.66, 130.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 293, 1432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3914) [M::mem_pestat] mean and std.dev: (820.80, 997.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691798 sequences (100000265 bp) in 3.060 CPU sec, 3.382 real sec... [M::mem_process_seqs] Processed 693202 reads in 101.185 CPU sec, 3.653 real sec sorting block 38, #reads = 1084462 mem = 536880146 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287452, 7, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 278, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (286.79, 100.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 253, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (275.78, 132.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 691554 sequences (100000004 bp) in 2.504 CPU sec, 2.804 real sec... [M::mem_process_seqs] Processed 694308 reads in 317.001 CPU sec, 10.917 real sec [M::mem_process_seqs] Processed 691798 reads in 100.846 CPU sec, 3.511 real sec sorting block 39, #reads = 1087035 mem = 536878878 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287756, 10, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 267, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (292.00, 276.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 255, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (277.57, 132.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 134, 3110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9208) [M::mem_pestat] mean and std.dev: (1125.30, 1361.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (298, 1032, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2500) [M::mem_pestat] mean and std.dev: (674.00, 489.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3234) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691072 sequences (100000263 bp) in 2.767 CPU sec, 3.123 real sec... [M::mem_process_seqs] Processed 691554 reads in 101.207 CPU sec, 3.535 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 287472, 4, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 247, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4257) [M::mem_pestat] mean and std.dev: (737.81, 864.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 256, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (279.63, 134.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 691106 sequences (100000083 bp) in 2.743 CPU sec, 3.547 real sec... [M::mem_process_seqs] Processed 691072 reads in 100.086 CPU sec, 3.606 real sec sorting block 40, #reads = 1086524 mem = 536878276 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 287695, 4, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 817, 4891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14111) [M::mem_pestat] mean and std.dev: (1949.00, 1975.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 258, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (281.26, 135.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 206, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2036) [M::mem_pestat] mean and std.dev: (325.89, 255.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690476 sequences (100000036 bp) in 2.548 CPU sec, 2.885 real sec... [M::mem_process_seqs] Processed 691106 reads in 98.642 CPU sec, 3.624 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287432, 6, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 335, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (146, 461) [M::mem_pestat] mean and std.dev: (295.83, 58.63) [M::mem_pestat] low and high boundaries for proper pairs: (61, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 259, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (282.97, 136.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 256, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (224.82, 87.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 41, #reads = 1086858 mem = 536879464 peak = 957366272 [M::process] read 690462 sequences (100000095 bp) in 2.573 CPU sec, 3.149 real sec... [M::mem_process_seqs] Processed 690476 reads in 100.693 CPU sec, 3.550 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 287427, 12, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 360, 1603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4089) [M::mem_pestat] mean and std.dev: (508.06, 459.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 261, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (285.40, 138.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 357, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (320.64, 211.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 183, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (208.07, 70.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690504 sequences (100000083 bp) in 2.798 CPU sec, 3.058 real sec... [M::mem_process_seqs] Processed 690462 reads in 98.732 CPU sec, 3.383 real sec sorting block 42, #reads = 1085994 mem = 536879275 peak = 957366272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 287543, 7, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 314, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (333.89, 152.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 260, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (284.55, 138.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 338, 1457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3957) [M::mem_pestat] mean and std.dev: (452.50, 580.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690054 sequences (100000159 bp) in 2.707 CPU sec, 3.090 real sec... [M::mem_process_seqs] Processed 690504 reads in 98.765 CPU sec, 3.480 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 287408, 2, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 473, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2705) [M::mem_pestat] mean and std.dev: (600.57, 504.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 262, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (286.84, 139.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 204, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (207.44, 93.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689938 sequences (100000104 bp) in 2.750 CPU sec, 3.109 real sec... sorting block 43, #reads = 1086167 mem = 536878044 peak = 962441216 [M::mem_process_seqs] Processed 690054 reads in 99.654 CPU sec, 3.500 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287304, 1, 15) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 263, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (287.27, 139.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 232, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 477) [M::mem_pestat] mean and std.dev: (242.71, 66.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RR [M::process] read 689400 sequences (100000201 bp) in 2.480 CPU sec, 2.978 real sec... [M::mem_process_seqs] Processed 689938 reads in 98.906 CPU sec, 3.486 real sec sorting block 44, #reads = 1085930 mem = 536880126 peak = 962441216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287037, 2, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 306, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2553) [M::mem_pestat] mean and std.dev: (516.92, 567.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 265, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (288.41, 139.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 235, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (215, 265) [M::mem_pestat] mean and std.dev: (237.50, 4.33) [M::mem_pestat] low and high boundaries for proper pairs: (205, 275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690092 sequences (100000222 bp) in 2.684 CPU sec, 3.375 real sec... [M::mem_process_seqs] Processed 689400 reads in 98.613 CPU sec, 3.469 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 287702, 1, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 220, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (329.75, 204.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 262, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (286.62, 139.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 234, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (271.38, 185.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 45, #reads = 1085599 mem = 536877326 peak = 962441216 [M::process] read 690612 sequences (100000288 bp) in 2.886 CPU sec, 3.341 real sec... [M::mem_process_seqs] Processed 690092 reads in 98.946 CPU sec, 3.400 real sec [M::process] read 689388 sequences (100000296 bp) in 2.505 CPU sec, 2.899 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287351, 8, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 213, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3052) [M::mem_pestat] mean and std.dev: (459.46, 542.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 258, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (282.73, 137.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (353, 379, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3023) [M::mem_pestat] mean and std.dev: (713.54, 584.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690612 reads in 99.852 CPU sec, 3.599 real sec sorting block 46, #reads = 1085399 mem = 536878177 peak = 962441216 [M::process] read 688792 sequences (100000141 bp) in 2.672 CPU sec, 3.023 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287321, 4, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 630, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1304) [M::mem_pestat] mean and std.dev: (462.36, 193.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 265, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (289.25, 140.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 286669, 3, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 237, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3826) [M::mem_pestat] mean and std.dev: (304.56, 295.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 266, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (289.08, 138.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 284, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (235.33, 79.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688340 sequences (100000059 bp) in 2.477 CPU sec, 2.902 real sec... [M::mem_process_seqs] Processed 689388 reads in 177.775 CPU sec, 5.918 real sec [M::mem_process_seqs] Processed 688792 reads in 117.387 CPU sec, 3.944 real sec sorting block 47, #reads = 1085686 mem = 536879822 peak = 962441216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287144, 4, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 266, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (288.71, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 404, 2178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6230) [M::mem_pestat] mean and std.dev: (926.00, 862.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8256) [M::mem_pestat] skip orientation RR [M::process] read 687970 sequences (100000120 bp) in 2.792 CPU sec, 3.301 real sec... [M::mem_process_seqs] Processed 688340 reads in 98.038 CPU sec, 3.461 real sec sorting block 48, #reads = 1086296 mem = 536880156 peak = 962441216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286375, 2, 20) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 268, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (291.26, 140.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 336, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207) [M::mem_pestat] mean and std.dev: (324.65, 167.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] skip orientation RR [M::process] read 688002 sequences (100000036 bp) in 2.966 CPU sec, 3.398 real sec... [M::mem_process_seqs] Processed 687970 reads in 97.421 CPU sec, 3.418 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286848, 2, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 184, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689) [M::mem_pestat] mean and std.dev: (319.69, 260.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 267, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (289.75, 138.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 209, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (88, 378) [M::mem_pestat] mean and std.dev: (223.10, 37.47) [M::mem_pestat] low and high boundaries for proper pairs: (30, 436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688262 sequences (100000090 bp) in 2.504 CPU sec, 2.839 real sec... [M::mem_process_seqs] Processed 688002 reads in 100.949 CPU sec, 3.510 real sec sorting block 49, #reads = 1085570 mem = 536878122 peak = 962441216 [M::process] read 688716 sequences (100000124 bp) in 2.568 CPU sec, 3.042 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286535, 4, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 898, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7469) [M::mem_pestat] mean and std.dev: (1258.80, 1038.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (287.72, 137.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 260, 4905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14205) [M::mem_pestat] mean and std.dev: (1967.27, 2890.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688262 reads in 97.159 CPU sec, 3.380 real sec sorting block 50, #reads = 1084343 mem = 536879609 peak = 962441216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286370, 5, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 263, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (286.68, 137.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 302, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2762) [M::mem_pestat] mean and std.dev: (594.27, 430.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3605) [M::mem_pestat] skip orientation RR [M::process] read 688448 sequences (100000054 bp) in 2.923 CPU sec, 3.255 real sec... [M::mem_process_seqs] Processed 688716 reads in 102.208 CPU sec, 3.431 real sec [M::process] read 689058 sequences (100000042 bp) in 2.398 CPU sec, 2.625 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 287061, 3, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 360, 2212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6256) [M::mem_pestat] mean and std.dev: (714.42, 834.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 262, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (285.66, 136.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 348, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192) [M::mem_pestat] mean and std.dev: (325.87, 151.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688448 reads in 96.652 CPU sec, 3.425 real sec sorting block 51, #reads = 1083671 mem = 536877868 peak = 962441216 [M::process] read 688714 sequences (100000091 bp) in 2.720 CPU sec, 3.056 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286867, 2, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 261, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (284.85, 136.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 689058 reads in 102.079 CPU sec, 3.531 real sec [M::process] read 688198 sequences (100000156 bp) in 2.470 CPU sec, 2.924 real sec... sorting block 52, #reads = 1084055 mem = 536879162 peak = 962441216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287363, 3, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 215, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2387) [M::mem_pestat] mean and std.dev: (474.08, 567.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 264, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (287.10, 137.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 245, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4024) [M::mem_pestat] mean and std.dev: (736.09, 851.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286641, 4, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 172, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (222.07, 97.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (287.62, 136.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 1321, 1689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4467) [M::mem_pestat] mean and std.dev: (1377.28, 1354.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6794) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687692 sequences (100000082 bp) in 2.796 CPU sec, 3.142 real sec... [M::mem_process_seqs] Processed 688714 reads in 179.479 CPU sec, 5.951 real sec [M::mem_process_seqs] Processed 688198 reads in 120.690 CPU sec, 3.993 real sec sorting block 53, #reads = 1083601 mem = 536880937 peak = 962441216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286776, 8, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 228, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626) [M::mem_pestat] mean and std.dev: (416.31, 404.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 267, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (288.88, 136.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 385, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2547) [M::mem_pestat] mean and std.dev: (462.81, 329.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3312) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687932 sequences (100000030 bp) in 2.850 CPU sec, 3.230 real sec... [M::mem_process_seqs] Processed 687692 reads in 98.033 CPU sec, 3.479 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287011, 7, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 268, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (290.71, 138.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::process] read 688214 sequences (100000139 bp) in 2.518 CPU sec, 3.096 real sec... [M::mem_process_seqs] Processed 687932 reads in 101.271 CPU sec, 3.564 real sec sorting block 54, #reads = 1083142 mem = 536878961 peak = 962441216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 286945, 3, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 415, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (380.78, 238.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 268, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (290.92, 139.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 318, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2429) [M::mem_pestat] mean and std.dev: (501.62, 333.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687970 sequences (100000287 bp) in 2.485 CPU sec, 2.844 real sec... [M::mem_process_seqs] Processed 688214 reads in 99.207 CPU sec, 3.541 real sec sorting block 55, #reads = 1081689 mem = 536879422 peak = 962441216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287127, 2, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 232, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2832) [M::mem_pestat] mean and std.dev: (463.17, 358.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 269, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (292.92, 141.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 692620 sequences (100000136 bp) in 3.010 CPU sec, 3.396 real sec... [M::mem_process_seqs] Processed 687970 reads in 104.689 CPU sec, 3.642 real sec [M::process] read 694760 sequences (100000189 bp) in 2.684 CPU sec, 3.027 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287730, 8, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 274, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2171) [M::mem_pestat] mean and std.dev: (323.08, 241.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 251, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (276.00, 134.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 1516, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3950) [M::mem_pestat] mean and std.dev: (1009.09, 698.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692620 reads in 106.915 CPU sec, 3.727 real sec sorting block 56, #reads = 1081652 mem = 536881717 peak = 962441216 [M::process] read 693448 sequences (100000294 bp) in 2.272 CPU sec, 2.657 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 288351, 9, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 263, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1797) [M::mem_pestat] mean and std.dev: (587.39, 596.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 240, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (264.43, 127.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (249.71, 113.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288233, 3, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 247, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (272.30, 133.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::process] read 691496 sequences (100000050 bp) in 2.685 CPU sec, 3.058 real sec... sorting block 57, #reads = 1081124 mem = 536880034 peak = 962441216 [M::mem_process_seqs] Processed 694760 reads in 176.325 CPU sec, 5.930 real sec [M::mem_process_seqs] Processed 693448 reads in 113.657 CPU sec, 3.824 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287614, 8, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 769, 3134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8902) [M::mem_pestat] mean and std.dev: (1764.60, 2344.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 691274 sequences (100000105 bp) in 2.668 CPU sec, 3.224 real sec... [M::mem_pestat] (25, 50, 75) percentile: (174, 253, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (278.17, 135.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 194, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131) [M::mem_pestat] mean and std.dev: (281.25, 247.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691496 reads in 97.047 CPU sec, 3.353 real sec sorting block 58, #reads = 1088206 mem = 536878976 peak = 962441216 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287494, 3, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 345, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (358.07, 147.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 257, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (280.96, 136.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 348, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (302.71, 99.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691170 sequences (100000012 bp) in 2.631 CPU sec, 3.055 real sec... [M::mem_process_seqs] Processed 691274 reads in 93.919 CPU sec, 3.234 real sec [M::process] read 690554 sequences (100000096 bp) in 2.230 CPU sec, 2.446 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 287750, 3, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 635, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3452) [M::mem_pestat] mean and std.dev: (591.55, 438.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 258, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (282.53, 137.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 188, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (186.89, 40.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 59, #reads = 1088408 mem = 536877266 peak = 974135296 [M::mem_process_seqs] Processed 691170 reads in 92.771 CPU sec, 3.232 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287407, 6, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 258, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (282.84, 137.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (377, 468, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (455.31, 180.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RR [M::process] read 690622 sequences (100000278 bp) in 2.669 CPU sec, 3.073 real sec... [M::mem_process_seqs] Processed 690554 reads in 97.362 CPU sec, 3.454 real sec sorting block 60, #reads = 1088545 mem = 536887194 peak = 974135296 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 287542, 4, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 245, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (267.23, 135.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 260, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (284.04, 138.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 250, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (280.50, 119.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689904 sequences (100000262 bp) in 2.711 CPU sec, 3.108 real sec... [M::mem_process_seqs] Processed 690622 reads in 94.181 CPU sec, 3.461 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 287336, 8, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 295, 1644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4558) [M::mem_pestat] mean and std.dev: (733.60, 751.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 263, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (287.56, 140.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 246, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1721) [M::mem_pestat] mean and std.dev: (341.36, 240.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689916 sequences (100000200 bp) in 2.570 CPU sec, 2.954 real sec... [M::mem_process_seqs] Processed 689904 reads in 98.862 CPU sec, 3.433 real sec sorting block 61, #reads = 1086495 mem = 536876815 peak = 974135296 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 287734, 7, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 411, 1733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4823) [M::mem_pestat] mean and std.dev: (991.88, 946.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 263, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (288.13, 140.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 279, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (264.81, 92.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689726 sequences (100000285 bp) in 2.406 CPU sec, 2.806 real sec... [M::mem_process_seqs] Processed 689916 reads in 94.320 CPU sec, 3.287 real sec sorting block 62, #reads = 1087556 mem = 536877534 peak = 974135296 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286950, 4, 20) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 263, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (288.19, 140.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 228, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (196.81, 43.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RR [M::process] read 689018 sequences (100000234 bp) in 2.853 CPU sec, 3.224 real sec... [M::mem_process_seqs] Processed 689726 reads in 98.614 CPU sec, 3.440 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287083, 10, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 266, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (290.42, 141.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (219, 481, 2271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6375) [M::mem_pestat] mean and std.dev: (1110.67, 1553.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8427) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 278, 1551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4253) [M::mem_pestat] mean and std.dev: (748.09, 837.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5604) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688630 sequences (100000240 bp) in 2.621 CPU sec, 3.056 real sec... [M::mem_process_seqs] Processed 689018 reads in 94.238 CPU sec, 3.240 real sec sorting block 63, #reads = 1085855 mem = 536878869 peak = 974135296 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286946, 6, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 250, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (240.12, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 266, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (291.12, 141.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 299, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1414) [M::mem_pestat] mean and std.dev: (395.90, 306.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689654 sequences (100000216 bp) in 2.623 CPU sec, 2.975 real sec... [M::mem_process_seqs] Processed 688630 reads in 98.107 CPU sec, 3.366 real sec sorting block 64, #reads = 1085440 mem = 536879555 peak = 983040000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287418, 3, 22) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 263, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (287.41, 139.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 283, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242) [M::mem_pestat] mean and std.dev: (351.67, 176.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1589) [M::mem_pestat] skip orientation RR [M::process] read 690140 sequences (100000237 bp) in 2.584 CPU sec, 3.041 real sec... [M::mem_process_seqs] Processed 689654 reads in 96.981 CPU sec, 3.281 real sec [M::process] read 688344 sequences (100000072 bp) in 2.531 CPU sec, 2.862 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 287691, 10, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 260, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (320.44, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (285.25, 138.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1509, 1914, 2421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4245) [M::mem_pestat] mean and std.dev: (1679.30, 700.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5157) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF sorting block 65, #reads = 1085450 mem = 536880332 peak = 983040000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 287079, 8, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 319, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (373.77, 209.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 268, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (291.81, 140.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 422, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (434.18, 260.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688466 sequences (100000094 bp) in 2.767 CPU sec, 3.172 real sec... [M::mem_process_seqs] Processed 690140 reads in 175.889 CPU sec, 5.720 real sec [M::mem_process_seqs] Processed 688344 reads in 115.973 CPU sec, 3.731 real sec sorting block 66, #reads = 1085916 mem = 536876304 peak = 992866304 [M::process] read 688148 sequences (100000053 bp) in 2.784 CPU sec, 3.111 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287544, 5, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 266, 4836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14146) [M::mem_pestat] mean and std.dev: (1712.92, 2100.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 269, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (292.45, 141.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286828, 8, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 168, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (172.00, 60.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 268, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (289.31, 137.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 253, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (207.89, 86.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687582 sequences (100000041 bp) in 2.657 CPU sec, 2.973 real sec... [M::mem_process_seqs] Processed 688466 reads in 176.742 CPU sec, 5.718 real sec [M::mem_process_seqs] Processed 688148 reads in 117.424 CPU sec, 3.771 real sec sorting block 67, #reads = 1085312 mem = 536877029 peak = 992866304 [M::process] read 687766 sequences (100000097 bp) in 2.247 CPU sec, 2.423 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286190, 10, 20) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 268, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (289.26, 136.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (437, 593, 1947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4967) [M::mem_pestat] mean and std.dev: (1054.20, 733.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 363, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1563) [M::mem_pestat] mean and std.dev: (400.25, 224.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1986) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688164 sequences (100000026 bp) in 2.229 CPU sec, 2.513 real sec... sorting block 68, #reads = 1084590 mem = 536879931 peak = 1031430144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 286865, 5, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 251, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1335) [M::mem_pestat] mean and std.dev: (362.23, 307.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 267, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (287.98, 135.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 245, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411) [M::mem_pestat] mean and std.dev: (319.56, 273.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687582 reads in 176.911 CPU sec, 5.703 real sec [M::mem_process_seqs] Processed 687766 reads in 117.362 CPU sec, 3.737 real sec [M::process] read 688230 sequences (100000134 bp) in 2.628 CPU sec, 2.745 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286805, 16, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 989, 3116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8878) [M::mem_pestat] mean and std.dev: (1315.58, 1530.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (286.06, 135.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (134, 1699, 8800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 26132) [M::mem_pestat] mean and std.dev: (3300.50, 3780.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 34798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 307, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (250.14, 110.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 69, #reads = 1083301 mem = 536885670 peak = 1031430144 [M::process] read 687962 sequences (100000255 bp) in 2.725 CPU sec, 3.246 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 286589, 3, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 217, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2214) [M::mem_pestat] mean and std.dev: (456.82, 479.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 264, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (286.04, 135.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 307, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247) [M::mem_pestat] mean and std.dev: (500.92, 482.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2945) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688164 reads in 173.002 CPU sec, 5.580 real sec [M::mem_process_seqs] Processed 688230 reads in 115.901 CPU sec, 3.698 real sec [M::process] read 688280 sequences (100000133 bp) in 2.687 CPU sec, 2.881 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286507, 9, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (400, 400, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (320, 520) [M::mem_pestat] mean and std.dev: (405.71, 14.00) [M::mem_pestat] low and high boundaries for proper pairs: (280, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 265, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (287.51, 136.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF sorting block 70, #reads = 1083865 mem = 536881070 peak = 1031430144 [M::process] read 687836 sequences (100000008 bp) in 2.769 CPU sec, 3.337 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 287391, 16, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 221, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 366) [M::mem_pestat] mean and std.dev: (194.08, 62.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (287.05, 135.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 28, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741) [M::mem_pestat] mean and std.dev: (337.47, 507.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2367) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 687962 reads in 175.082 CPU sec, 5.749 real sec [M::mem_process_seqs] Processed 688280 reads in 116.052 CPU sec, 3.819 real sec sorting block 71, #reads = 1083796 mem = 536881837 peak = 1031430144 [M::process] read 688098 sequences (100000247 bp) in 2.982 CPU sec, 3.193 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286829, 0, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 350, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (335.64, 177.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 266, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (287.98, 136.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1309, 2152, 3817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8833) [M::mem_pestat] mean and std.dev: (2305.00, 1523.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687718 sequences (100000192 bp) in 2.465 CPU sec, 2.779 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286885, 4, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 202, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 350) [M::mem_pestat] mean and std.dev: (192.07, 48.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 266, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (287.66, 136.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 295, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (332.73, 164.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687836 reads in 176.573 CPU sec, 5.730 real sec [M::mem_process_seqs] Processed 688098 reads in 116.929 CPU sec, 3.759 real sec sorting block 72, #reads = 1083970 mem = 536878721 peak = 1031430144 [M::process] read 687582 sequences (100000262 bp) in 2.986 CPU sec, 3.212 real sec... sorting block 73, #reads = 1083740 mem = 536881590 peak = 1031430144 [M::process] read 687436 sequences (100000035 bp) in 2.602 CPU sec, 2.816 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286383, 5, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 191, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2880) [M::mem_pestat] mean and std.dev: (506.00, 524.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 268, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (291.51, 139.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 259, 1325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3659) [M::mem_pestat] mean and std.dev: (494.31, 467.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687582 reads in 100.846 CPU sec, 3.208 real sec [M::process] read 687750 sequences (100000164 bp) in 2.485 CPU sec, 2.718 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287251, 5, 19) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 268, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (289.79, 137.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 291, 2195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6283) [M::mem_pestat] mean and std.dev: (572.50, 887.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8327) [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286527, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 269, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (292.39, 140.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 687718 reads in 277.454 CPU sec, 8.917 real sec sorting block 74, #reads = 1082655 mem = 536879249 peak = 1031430144 [M::process] read 691738 sequences (100000113 bp) in 2.982 CPU sec, 3.205 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286152, 7, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 350, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1408) [M::mem_pestat] mean and std.dev: (316.00, 170.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 269, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (291.83, 139.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 687750 reads in 101.111 CPU sec, 3.229 real sec [M::process] read 697226 sequences (100000104 bp) in 2.394 CPU sec, 2.569 real sec... [M::mem_process_seqs] Processed 687436 reads in 295.548 CPU sec, 9.430 real sec sorting block 75, #reads = 1083027 mem = 536881514 peak = 1031430144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287946, 1, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 251, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (277.06, 135.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 429, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1787) [M::mem_pestat] mean and std.dev: (368.18, 173.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2294) [M::mem_pestat] skip orientation RR [M::process] read 695520 sequences (100000196 bp) in 2.913 CPU sec, 3.118 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289217, 2, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 182, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (296.00, 209.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 232, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (257.41, 125.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 395, 1766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4890) [M::mem_pestat] mean and std.dev: (637.90, 629.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691738 reads in 220.234 CPU sec, 7.015 real sec [M::process] read 695644 sequences (100000077 bp) in 2.061 CPU sec, 2.148 real sec... [M::mem_process_seqs] Processed 697226 reads in 132.978 CPU sec, 4.241 real sec sorting block 76, #reads = 1083334 mem = 536878219 peak = 1031430144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288697, 8, 17) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 239, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (265.60, 130.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 289, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3827) [M::mem_pestat] mean and std.dev: (828.00, 990.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5035) [M::mem_pestat] skip orientation RR [M::process] read 695582 sequences (100000018 bp) in 2.994 CPU sec, 3.385 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289173, 8, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 279, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (99, 399) [M::mem_pestat] mean and std.dev: (236.82, 56.38) [M::mem_pestat] low and high boundaries for proper pairs: (11, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 240, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (266.92, 132.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 695520 reads in 195.349 CPU sec, 6.278 real sec sorting block 77, #reads = 1083239 mem = 536880690 peak = 1031430144 [M::process] read 694822 sequences (100000153 bp) in 2.146 CPU sec, 2.169 real sec... [M::mem_process_seqs] Processed 695644 reads in 124.969 CPU sec, 3.968 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 289775, 5, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 141, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (198.62, 122.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 239, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (266.37, 130.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF sorting block 78, #reads = 1084892 mem = 536878305 peak = 1094332416 [M::process] read 693952 sequences (100000096 bp) in 2.835 CPU sec, 3.203 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 289131, 3, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 203, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (265.08, 175.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 242, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (269.23, 132.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 278, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (249.62, 96.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695582 reads in 189.316 CPU sec, 6.126 real sec [M::process] read 693068 sequences (100000133 bp) in 1.992 CPU sec, 2.159 real sec... [M::mem_process_seqs] Processed 694822 reads in 121.954 CPU sec, 3.902 real sec sorting block 79, #reads = 1088110 mem = 536879204 peak = 1094332416 [M::process] read 693258 sequences (100000041 bp) in 2.759 CPU sec, 3.180 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 288390, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 369, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (296.75, 116.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (275.23, 135.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 693068 reads in 103.952 CPU sec, 3.328 real sec [M::process] read 692748 sequences (100000256 bp) in 2.252 CPU sec, 2.363 real sec... sorting block 80, #reads = 1086458 mem = 536878250 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288847, 7, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (273.78, 134.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 247, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (235.45, 88.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 288662, 4, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 229, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (203.50, 68.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (275.63, 135.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 693952 reads in 289.980 CPU sec, 9.339 real sec [M::mem_process_seqs] Processed 693258 reads in 141.682 CPU sec, 4.526 real sec [M::process] read 692562 sequences (100000228 bp) in 2.307 CPU sec, 2.399 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 288461, 12, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 234, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2072) [M::mem_pestat] mean and std.dev: (348.38, 262.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2697) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 81, #reads = 1085799 mem = 536882363 peak = 1094332416 [M::mem_pestat] (25, 50, 75) percentile: (171, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (277.37, 136.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1218, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2424) [M::mem_pestat] mean and std.dev: (873.45, 472.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3027) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 307, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (256.67, 78.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692128 sequences (100000215 bp) in 2.647 CPU sec, 3.009 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288320, 10, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 252, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (278.60, 137.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (4, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (95.89, 88.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 692748 reads in 184.734 CPU sec, 5.957 real sec [M::mem_process_seqs] Processed 692562 reads in 121.700 CPU sec, 3.875 real sec [M::process] read 692612 sequences (100000264 bp) in 2.690 CPU sec, 2.827 real sec... sorting block 82, #reads = 1086063 mem = 536878426 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288244, 17, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 254, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (279.41, 136.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 377, 3212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9582) [M::mem_pestat] mean and std.dev: (1362.18, 1840.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 235, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (261.09, 160.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692220 sequences (100000071 bp) in 2.771 CPU sec, 3.122 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 288161, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 329, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026) [M::mem_pestat] mean and std.dev: (324.45, 170.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 252, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (278.77, 137.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 442, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4177) [M::mem_pestat] mean and std.dev: (774.05, 610.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5518) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692128 reads in 184.491 CPU sec, 5.932 real sec [M::mem_process_seqs] Processed 692612 reads in 124.349 CPU sec, 3.959 real sec [M::process] read 692100 sequences (100000223 bp) in 2.149 CPU sec, 2.233 real sec... sorting block 83, #reads = 1086060 mem = 536879567 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288181, 2, 15) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 253, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (278.94, 136.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.07, 47.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RR [M::process] read 692180 sequences (100000049 bp) in 2.856 CPU sec, 3.342 real sec... sorting block 84, #reads = 1085259 mem = 536877490 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 288476, 2, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 235, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378) [M::mem_pestat] mean and std.dev: (285.18, 161.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (280.16, 137.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 302, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (325.50, 204.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692220 reads in 184.441 CPU sec, 5.942 real sec [M::mem_process_seqs] Processed 692100 reads in 123.359 CPU sec, 3.925 real sec [M::process] read 691712 sequences (100000049 bp) in 2.330 CPU sec, 2.483 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 288395, 9, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 229, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2445) [M::mem_pestat] mean and std.dev: (511.96, 437.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (280.38, 137.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 237, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (313.23, 207.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 85, #reads = 1085294 mem = 536884079 peak = 1094332416 [M::process] read 691654 sequences (100000004 bp) in 2.787 CPU sec, 3.057 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287866, 3, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 313, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (249.56, 77.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 254, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (281.00, 138.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 306, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (272.62, 138.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692180 reads in 188.194 CPU sec, 6.059 real sec [M::mem_process_seqs] Processed 691712 reads in 123.582 CPU sec, 3.926 real sec [M::process] read 691420 sequences (100000131 bp) in 2.318 CPU sec, 2.380 real sec... sorting block 86, #reads = 1084027 mem = 536878209 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288315, 0, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 254, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (281.16, 137.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 283, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (244.31, 88.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RR [M::process] read 691086 sequences (100000146 bp) in 2.687 CPU sec, 2.914 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287582, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 236, 1575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4425) [M::mem_pestat] mean and std.dev: (757.27, 786.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (283.19, 139.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 339, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1589) [M::mem_pestat] mean and std.dev: (433.96, 248.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691654 reads in 187.117 CPU sec, 6.048 real sec [M::mem_process_seqs] Processed 691420 reads in 123.202 CPU sec, 3.951 real sec [M::process] read 690226 sequences (100000038 bp) in 2.773 CPU sec, 2.932 real sec... sorting block 87, #reads = 1084244 mem = 536882533 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287922, 12, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 258, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (284.09, 139.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 1934, 1934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5744) [M::mem_pestat] mean and std.dev: (1248.00, 1052.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (405, 623, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (585.80, 250.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1681) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690004 sequences (100000205 bp) in 2.515 CPU sec, 2.970 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287629, 5, 19) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (284.52, 137.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 409, 3056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8722) [M::mem_pestat] mean and std.dev: (1328.84, 1797.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11555) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691086 reads in 189.058 CPU sec, 6.126 real sec sorting block 88, #reads = 1084345 mem = 536881134 peak = 1094332416 [M::mem_process_seqs] Processed 690226 reads in 124.354 CPU sec, 3.999 real sec [M::process] read 689506 sequences (100000081 bp) in 2.298 CPU sec, 2.440 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287624, 7, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 169, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (198.62, 70.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (284.77, 137.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF sorting block 89, #reads = 1083240 mem = 536880264 peak = 1094332416 [M::process] read 689056 sequences (100000183 bp) in 2.776 CPU sec, 3.064 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 286888, 6, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 233, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1787) [M::mem_pestat] mean and std.dev: (423.05, 390.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 262, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (285.82, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 690004 reads in 192.321 CPU sec, 6.229 real sec [M::mem_process_seqs] Processed 689506 reads in 125.315 CPU sec, 4.035 real sec [M::process] read 689274 sequences (100000047 bp) in 2.568 CPU sec, 2.784 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286953, 5, 17) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 263, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (286.75, 137.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (387, 387, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (267, 567) [M::mem_pestat] mean and std.dev: (420.92, 47.20) [M::mem_pestat] low and high boundaries for proper pairs: (207, 627) [M::mem_pestat] skip orientation RR sorting block 90, #reads = 1081516 mem = 536878172 peak = 1094332416 [M::process] read 688294 sequences (100000217 bp) in 2.586 CPU sec, 3.063 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286786, 9, 16) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 263, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (287.05, 137.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 320, 908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (528.40, 586.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3089) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689056 reads in 192.058 CPU sec, 6.281 real sec [M::mem_process_seqs] Processed 689274 reads in 125.535 CPU sec, 4.082 real sec [M::process] read 688604 sequences (100000180 bp) in 2.312 CPU sec, 2.539 real sec... sorting block 91, #reads = 1081396 mem = 536879840 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287016, 8, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 493, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 753) [M::mem_pestat] mean and std.dev: (424.80, 103.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 267, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (289.95, 138.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 688820 sequences (100000009 bp) in 2.723 CPU sec, 3.084 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 286575, 6, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 317, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1616) [M::mem_pestat] mean and std.dev: (459.79, 253.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 265, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (288.46, 138.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 688294 reads in 192.855 CPU sec, 6.220 real sec [M::mem_process_seqs] Processed 688604 reads in 124.853 CPU sec, 3.966 real sec [M::process] read 689248 sequences (100000150 bp) in 2.522 CPU sec, 2.615 real sec... sorting block 92, #reads = 1081785 mem = 536878842 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 287169, 5, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 233, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (249.95, 144.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 264, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (288.72, 139.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 553, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792) [M::mem_pestat] mean and std.dev: (499.36, 351.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 702954 sequences (100000182 bp) in 2.799 CPU sec, 3.136 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286566, 5, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 262, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (286.96, 139.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 688820 reads in 195.521 CPU sec, 6.274 real sec sorting block 93, #reads = 1080637 mem = 536881000 peak = 1094332416 [M::process] read 700804 sequences (100000142 bp) in 2.223 CPU sec, 2.313 real sec... [M::mem_process_seqs] Processed 689248 reads in 127.394 CPU sec, 4.092 real sec sorting block 94, #reads = 1080764 mem = 536878752 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291624, 3, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 227, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (186.80, 97.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 217, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (239.88, 113.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 298, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (285.67, 132.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 700404 sequences (100000167 bp) in 3.002 CPU sec, 3.249 real sec... [M::process] read 698982 sequences (100000291 bp) in 2.105 CPU sec, 2.115 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 290514, 6, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 252, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (229.88, 67.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 224, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (248.23, 119.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 233, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (196.42, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702954 reads in 230.559 CPU sec, 7.395 real sec [M::mem_process_seqs] Processed 700804 reads in 150.417 CPU sec, 4.808 real sec sorting block 95, #reads = 1077614 mem = 536880678 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290679, 6, 17) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 224, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (249.74, 121.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 1874, 3541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10227) [M::mem_pestat] mean and std.dev: (1536.71, 1428.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13570) [M::mem_pestat] skip orientation RR [M::process] read 698212 sequences (100000288 bp) in 2.957 CPU sec, 3.443 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290347, 8, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (253.68, 123.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::process] read 696804 sequences (100000188 bp) in 2.075 CPU sec, 2.085 real sec... [M::mem_process_seqs] Processed 700404 reads in 220.675 CPU sec, 7.180 real sec [M::mem_process_seqs] Processed 698982 reads in 144.329 CPU sec, 4.665 real sec sorting block 96, #reads = 1078678 mem = 536879749 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 289946, 3, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 190, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729) [M::mem_pestat] mean and std.dev: (343.08, 324.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 231, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (257.00, 125.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 235, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (217.10, 45.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696366 sequences (100000177 bp) in 3.060 CPU sec, 3.532 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 289326, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 245, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (223.32, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 236, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (261.43, 126.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 284, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 495) [M::mem_pestat] mean and std.dev: (246.48, 75.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695340 sequences (100000112 bp) in 2.238 CPU sec, 2.247 real sec... [M::mem_process_seqs] Processed 698212 reads in 215.163 CPU sec, 6.948 real sec sorting block 97, #reads = 1079182 mem = 536878057 peak = 1094332416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 289817, 6, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 319, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 593) [M::mem_pestat] mean and std.dev: (283.67, 89.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 237, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (263.55, 128.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 694348 sequences (100000114 bp) in 2.370 CPU sec, 2.456 real sec... [M::mem_process_seqs] Processed 696366 reads in 119.887 CPU sec, 4.115 real sec sorting block 98, #reads = 1080906 mem = 536878340 peak = 1094332416 [M::mem_process_seqs] Processed 696804 reads in 304.874 CPU sec, 10.056 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 289359, 3, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 269, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (250.44, 81.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 240, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (265.82, 129.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 364, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531) [M::mem_pestat] mean and std.dev: (342.20, 184.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694132 sequences (100000075 bp) in 3.227 CPU sec, 3.442 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 289060, 6, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 463, 2247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6391) [M::mem_pestat] mean and std.dev: (1235.11, 1438.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 243, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (268.11, 129.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 236, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (232.82, 75.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695340 reads in 232.205 CPU sec, 7.532 real sec [M::process] read 693924 sequences (100000014 bp) in 2.181 CPU sec, 2.249 real sec... sorting block 99, #reads = 1080947 mem = 536877488 peak = 1094332416 [M::mem_process_seqs] Processed 694348 reads in 138.714 CPU sec, 4.448 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 288893, 9, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 245, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (270.51, 131.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs sorting block 100, #reads = 1081383 mem = 536878972 peak = 1094729728 [M::process] read 693530 sequences (100000195 bp) in 2.644 CPU sec, 2.914 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288826, 1, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 245, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (271.13, 131.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::process] read 693024 sequences (100000152 bp) in 2.256 CPU sec, 2.293 real sec... [M::mem_process_seqs] Processed 694132 reads in 204.586 CPU sec, 6.576 real sec [M::mem_process_seqs] Processed 693924 reads in 133.717 CPU sec, 4.279 real sec sorting block 101, #reads = 1081969 mem = 536879859 peak = 1094729728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 289115, 6, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 593, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (523.31, 276.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 246, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (272.34, 132.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 693108 sequences (100000140 bp) in 3.309 CPU sec, 3.761 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 288646, 4, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 210, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 377) [M::mem_pestat] mean and std.dev: (208.90, 43.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 248, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (273.55, 132.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 292, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1606) [M::mem_pestat] mean and std.dev: (394.46, 285.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693530 reads in 200.731 CPU sec, 6.474 real sec [M::process] read 692868 sequences (100000296 bp) in 2.058 CPU sec, 2.187 real sec... [M::mem_process_seqs] Processed 693024 reads in 130.573 CPU sec, 4.214 real sec sorting block 102, #reads = 1082936 mem = 536878623 peak = 1094729728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 288334, 7, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 371, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1543) [M::mem_pestat] mean and std.dev: (451.47, 346.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 248, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (273.61, 133.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 262, 2240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6298) [M::mem_pestat] mean and std.dev: (940.17, 1081.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692378 sequences (100000217 bp) in 3.234 CPU sec, 3.498 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 288958, 9, 28) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (275.74, 134.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 269, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (263.57, 110.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693108 reads in 199.756 CPU sec, 6.431 real sec [M::mem_process_seqs] Processed 692868 reads in 129.346 CPU sec, 4.105 real sec [M::process] read 691342 sequences (100000221 bp) in 2.432 CPU sec, 2.563 real sec... sorting block 103, #reads = 1083753 mem = 536878537 peak = 1094729728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288242, 7, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (277.58, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::process] read 690724 sequences (100000270 bp) in 3.244 CPU sec, 3.561 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287881, 8, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 203, 1366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3848) [M::mem_pestat] mean and std.dev: (456.00, 497.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 254, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (282.67, 139.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 197, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 303) [M::mem_pestat] mean and std.dev: (187.40, 31.79) [M::mem_pestat] low and high boundaries for proper pairs: (21, 350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692378 reads in 196.163 CPU sec, 6.326 real sec sorting block 104, #reads = 1083558 mem = 536878539 peak = 1094729728 [M::mem_process_seqs] Processed 691342 reads in 127.557 CPU sec, 4.080 real sec [M::process] read 690176 sequences (100000035 bp) in 2.178 CPU sec, 2.305 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287778, 4, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 257, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (285.00, 140.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 264, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (411.27, 275.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2095) [M::mem_pestat] skip orientation RR sorting block 105, #reads = 1082204 mem = 536879814 peak = 1094881280 [M::process] read 690098 sequences (100000187 bp) in 3.031 CPU sec, 3.313 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287887, 4, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 206, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (241.00, 170.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 258, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (285.29, 140.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 236, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 417) [M::mem_pestat] mean and std.dev: (200.82, 74.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690724 reads in 192.358 CPU sec, 6.190 real sec [M::process] read 689830 sequences (100000241 bp) in 2.355 CPU sec, 2.419 real sec... [M::mem_process_seqs] Processed 690176 reads in 126.068 CPU sec, 4.004 real sec sorting block 106, #reads = 1082486 mem = 536879309 peak = 1094881280 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287279, 8, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (222.64, 113.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 258, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (284.86, 139.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 238, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (271.79, 127.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689476 sequences (100000271 bp) in 3.009 CPU sec, 3.653 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287502, 15, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 243, 3662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10538) [M::mem_pestat] mean and std.dev: (1254.93, 1506.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 259, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (285.11, 139.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (172, 1348, 1458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4030) [M::mem_pestat] mean and std.dev: (961.87, 722.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 286, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (237.00, 83.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690098 reads in 190.943 CPU sec, 6.196 real sec [M::mem_process_seqs] Processed 689830 reads in 125.100 CPU sec, 4.017 real sec [M::process] read 689368 sequences (100000010 bp) in 2.385 CPU sec, 2.580 real sec... sorting block 107, #reads = 1082811 mem = 536879757 peak = 1094881280 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 287235, 8, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 183, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (316.58, 241.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 261, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (287.65, 140.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 689034 sequences (100000246 bp) in 2.724 CPU sec, 3.161 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286829, 3, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 213, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (232.33, 94.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 261, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (288.48, 141.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 373, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3218) [M::mem_pestat] mean and std.dev: (697.75, 697.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689476 reads in 192.457 CPU sec, 6.227 real sec [M::process] read 689164 sequences (100000046 bp) in 2.128 CPU sec, 2.161 real sec... [M::mem_process_seqs] Processed 689368 reads in 126.562 CPU sec, 4.081 real sec sorting block 108, #reads = 1083388 mem = 536879808 peak = 1094881280 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 287368, 10, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 340, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (309.57, 131.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 262, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (288.91, 141.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (493, 493, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1990) [M::mem_pestat] mean and std.dev: (683.80, 421.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2489) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (116, 184, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (181.50, 70.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690862 sequences (100000198 bp) in 3.019 CPU sec, 3.630 real sec... sorting block 109, #reads = 1080791 mem = 536881277 peak = 1094881280 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 286743, 8, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 238, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4227) [M::mem_pestat] mean and std.dev: (707.69, 833.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 264, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (290.96, 143.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 211, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (218.40, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689034 reads in 194.468 CPU sec, 6.357 real sec [M::mem_process_seqs] Processed 689164 reads in 130.026 CPU sec, 4.230 real sec [M::process] read 697428 sequences (100000104 bp) in 2.465 CPU sec, 2.591 real sec... sorting block 110, #reads = 1081707 mem = 536878163 peak = 1094881280 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287340, 8, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 278, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (152, 362) [M::mem_pestat] mean and std.dev: (247.38, 25.98) [M::mem_pestat] low and high boundaries for proper pairs: (110, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 255, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (282.60, 139.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 230, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (295.00, 178.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695722 sequences (100000226 bp) in 2.896 CPU sec, 3.327 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289664, 6, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 216, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2283) [M::mem_pestat] mean and std.dev: (429.64, 410.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 233, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (258.61, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1838) [M::mem_pestat] mean and std.dev: (383.18, 345.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2402) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694262 sequences (100000208 bp) in 2.003 CPU sec, 2.011 real sec... [M::mem_process_seqs] Processed 690862 reads in 218.367 CPU sec, 7.035 real sec [M::mem_process_seqs] Processed 697428 reads in 138.593 CPU sec, 4.431 real sec sorting block 111, #reads = 1073740 mem = 536884245 peak = 1094881280 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 289271, 4, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 239, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (263.92, 128.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::process] read 694188 sequences (100000128 bp) in 2.763 CPU sec, 3.255 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288888, 19, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 242, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (229.30, 103.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 243, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (268.82, 131.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (344, 416, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757) [M::mem_pestat] mean and std.dev: (552.00, 334.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2228) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 695722 reads in 189.797 CPU sec, 6.132 real sec [M::process] read 693570 sequences (100000203 bp) in 2.061 CPU sec, 2.093 real sec... [M::mem_process_seqs] Processed 694262 reads in 124.387 CPU sec, 3.978 real sec sorting block 112, #reads = 1083127 mem = 536876532 peak = 1094881280 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 289209, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 222, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (318.79, 246.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 245, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (270.34, 131.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 213, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (281.71, 178.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693082 sequences (100000113 bp) in 2.714 CPU sec, 3.081 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288886, 10, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 295, 3200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9296) [M::mem_pestat] mean and std.dev: (1274.60, 1546.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 246, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (272.20, 132.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 124, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (215.00, 220.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2148) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 334, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1892) [M::mem_pestat] mean and std.dev: (505.79, 454.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694188 reads in 185.677 CPU sec, 6.079 real sec sorting block 113, #reads = 1086629 mem = 536876230 peak = 1094881280 [M::process] read 692542 sequences (100000113 bp) in 2.262 CPU sec, 2.322 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 288896, 3, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 230, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (227.33, 65.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 248, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (272.94, 132.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 693082 reads in 100.573 CPU sec, 3.434 real sec [M::process] read 692644 sequences (100000154 bp) in 2.537 CPU sec, 2.777 real sec... sorting block 114, #reads = 1086399 mem = 536879999 peak = 1102532608 [M::mem_process_seqs] Processed 693570 reads in 272.913 CPU sec, 8.977 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288330, 8, 26) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 250, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (275.02, 133.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 441, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (358.91, 196.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] skip orientation RR [M::process] read 691878 sequences (100000071 bp) in 3.230 CPU sec, 3.530 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 288708, 5, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 416, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2358) [M::mem_pestat] mean and std.dev: (448.33, 317.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 250, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (274.87, 133.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 413, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2268) [M::mem_pestat] mean and std.dev: (553.08, 372.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692542 reads in 204.537 CPU sec, 6.629 real sec sorting block 115, #reads = 1085361 mem = 536878857 peak = 1102532608 [M::mem_process_seqs] Processed 692644 reads in 123.823 CPU sec, 4.010 real sec [M::process] read 691480 sequences (100000131 bp) in 2.457 CPU sec, 2.630 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 288084, 5, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 139, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (225.48, 164.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 253, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (277.13, 134.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 252, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (247.00, 135.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 116, #reads = 1084818 mem = 536884011 peak = 1102532608 [M::process] read 691526 sequences (100000254 bp) in 2.910 CPU sec, 3.275 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287968, 6, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (153.40, 38.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (279.37, 135.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 370, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1822) [M::mem_pestat] mean and std.dev: (369.86, 245.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691878 reads in 187.723 CPU sec, 6.072 real sec [M::mem_process_seqs] Processed 691480 reads in 123.576 CPU sec, 3.968 real sec [M::process] read 691244 sequences (100000163 bp) in 2.471 CPU sec, 2.627 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288051, 5, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 254, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (278.92, 135.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 202, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1529) [M::mem_pestat] mean and std.dev: (370.00, 296.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1981) [M::mem_pestat] skip orientation RR sorting block 117, #reads = 1086356 mem = 536880015 peak = 1102532608 [M::process] read 692144 sequences (100000142 bp) in 2.989 CPU sec, 3.569 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287733, 8, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 227, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1998) [M::mem_pestat] mean and std.dev: (340.55, 264.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 255, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (280.31, 136.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (400, 449, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (430.11, 175.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691526 reads in 185.744 CPU sec, 6.020 real sec [M::mem_process_seqs] Processed 691244 reads in 123.426 CPU sec, 3.941 real sec [M::process] read 691890 sequences (100000037 bp) in 2.420 CPU sec, 2.548 real sec... sorting block 118, #reads = 1084235 mem = 536877222 peak = 1102532608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 288551, 4, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 133, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (157.83, 63.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 251, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (275.83, 133.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 692048 sequences (100000170 bp) in 2.907 CPU sec, 3.373 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288367, 5, 18) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 250, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (275.59, 133.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 773, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3451) [M::mem_pestat] mean and std.dev: (964.22, 865.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4538) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692144 reads in 187.757 CPU sec, 6.066 real sec [M::mem_process_seqs] Processed 691890 reads in 124.963 CPU sec, 3.990 real sec sorting block 119, #reads = 1086315 mem = 536877865 peak = 1102532608 [M::process] read 691580 sequences (100000075 bp) in 2.732 CPU sec, 2.857 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 288625, 3, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 264, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (233.42, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (278.01, 136.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 253, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (359.33, 247.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690602 sequences (100000136 bp) in 2.655 CPU sec, 3.047 real sec... sorting block 120, #reads = 1085447 mem = 536886235 peak = 1102532608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287733, 3, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 225, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3788) [M::mem_pestat] mean and std.dev: (634.23, 665.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (280.62, 138.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 175, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909) [M::mem_pestat] mean and std.dev: (458.93, 406.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692048 reads in 187.942 CPU sec, 6.090 real sec [M::mem_process_seqs] Processed 691580 reads in 126.411 CPU sec, 4.079 real sec [M::process] read 690304 sequences (100000112 bp) in 2.139 CPU sec, 2.263 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 287935, 10, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 288, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3331) [M::mem_pestat] mean and std.dev: (414.29, 461.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 257, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (282.52, 137.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1024, 5888, 5888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15616) [M::mem_pestat] mean and std.dev: (3686.90, 2545.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF sorting block 121, #reads = 1084533 mem = 536879577 peak = 1102532608 [M::process] read 689844 sequences (100000085 bp) in 2.523 CPU sec, 3.016 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287593, 8, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 267, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (340.69, 204.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (285.97, 140.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 396, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (402.57, 185.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690602 reads in 184.551 CPU sec, 5.999 real sec [M::mem_process_seqs] Processed 690304 reads in 124.324 CPU sec, 4.001 real sec [M::process] read 689514 sequences (100000072 bp) in 2.976 CPU sec, 3.267 real sec... sorting block 122, #reads = 1083709 mem = 536877797 peak = 1102532608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287446, 3, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (286.71, 140.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::process] read 689434 sequences (100000168 bp) in 2.469 CPU sec, 3.107 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286823, 16, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 262, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671) [M::mem_pestat] mean and std.dev: (346.25, 228.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 260, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (287.25, 140.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 362) [M::mem_pestat] mean and std.dev: (186.25, 59.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 290, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (249.58, 55.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689844 reads in 188.619 CPU sec, 6.141 real sec [M::mem_process_seqs] Processed 689514 reads in 125.452 CPU sec, 4.055 real sec [M::process] read 690260 sequences (100000142 bp) in 2.617 CPU sec, 2.786 real sec... sorting block 123, #reads = 1082823 mem = 536877167 peak = 1102532608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287272, 9, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 236, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420) [M::mem_pestat] mean and std.dev: (217.11, 136.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 261, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (287.24, 140.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 689520 sequences (100000009 bp) in 2.916 CPU sec, 3.265 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287568, 4, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 1056, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3542) [M::mem_pestat] mean and std.dev: (829.90, 557.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 255, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (281.84, 137.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 362, 1225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3373) [M::mem_pestat] mean and std.dev: (739.43, 778.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689434 reads in 191.773 CPU sec, 6.185 real sec [M::mem_process_seqs] Processed 690260 reads in 127.035 CPU sec, 4.067 real sec sorting block 124, #reads = 1082649 mem = 536877308 peak = 1102532608 [M::process] read 689272 sequences (100000033 bp) in 2.148 CPU sec, 2.256 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 287188, 4, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (539, 1194, 2211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5555) [M::mem_pestat] mean and std.dev: (1286.50, 1181.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 260, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (286.07, 139.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 447, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2309) [M::mem_pestat] mean and std.dev: (413.09, 293.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 125, #reads = 1082545 mem = 536877535 peak = 1110528000 [M::process] read 689138 sequences (100000295 bp) in 3.067 CPU sec, 3.516 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286895, 6, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 261, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (286.77, 139.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (407, 583, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2063) [M::mem_pestat] mean and std.dev: (485.50, 254.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2615) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689520 reads in 192.158 CPU sec, 6.257 real sec [M::process] read 689490 sequences (100000089 bp) in 2.187 CPU sec, 2.265 real sec... [M::mem_process_seqs] Processed 689272 reads in 127.897 CPU sec, 4.127 real sec sorting block 126, #reads = 1081982 mem = 536878153 peak = 1110528000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 287119, 7, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 244, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (270.64, 139.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 261, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (286.84, 140.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 199, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (188.56, 47.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689096 sequences (100000050 bp) in 2.598 CPU sec, 3.088 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287351, 8, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 241, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2574) [M::mem_pestat] mean and std.dev: (698.90, 862.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (286.25, 139.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 365, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1249) [M::mem_pestat] mean and std.dev: (275.50, 135.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689138 reads in 194.984 CPU sec, 6.484 real sec [M::process] read 689358 sequences (100000197 bp) in 1.989 CPU sec, 2.184 real sec... [M::mem_process_seqs] Processed 689490 reads in 130.400 CPU sec, 4.207 real sec sorting block 127, #reads = 1080893 mem = 536881921 peak = 1110528000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287029, 5, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 231, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (220.00, 69.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 261, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (287.46, 140.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 286, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (328.25, 118.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695960 sequences (100000274 bp) in 3.069 CPU sec, 3.571 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287278, 3, 17) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 261, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (287.73, 141.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 403, 2291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6429) [M::mem_pestat] mean and std.dev: (1146.35, 1176.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8498) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689096 reads in 203.709 CPU sec, 6.589 real sec [M::process] read 703250 sequences (100000297 bp) in 2.266 CPU sec, 2.324 real sec... sorting block 128, #reads = 1080137 mem = 536879246 peak = 1110528000 [M::process] read 699006 sequences (100000014 bp) in 2.306 CPU sec, 2.320 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290919, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 225, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (238.17, 107.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 218, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (241.43, 116.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 703250 reads in 116.163 CPU sec, 3.800 real sec sorting block 129, #reads = 1078095 mem = 536882245 peak = 1110528000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 288951, 7, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 259, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1822) [M::mem_pestat] mean and std.dev: (411.00, 370.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 237, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (263.48, 129.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 319, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (372.71, 202.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695960 reads in 248.642 CPU sec, 8.795 real sec [M::process] read 697556 sequences (100000019 bp) in 2.383 CPU sec, 2.481 real sec... [M::mem_process_seqs] Processed 689358 reads in 447.281 CPU sec, 15.112 real sec sorting block 130, #reads = 1072244 mem = 536879784 peak = 1110528000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290249, 6, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 244, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (266.82, 103.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 229, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (255.57, 125.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 242, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (88, 368) [M::mem_pestat] mean and std.dev: (220.44, 63.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696752 sequences (100000081 bp) in 2.085 CPU sec, 2.468 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290056, 5, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 234, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (260.48, 127.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 320, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (309.46, 127.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RR [M::process] read 695114 sequences (100000127 bp) in 2.145 CPU sec, 2.147 real sec... [M::mem_process_seqs] Processed 699006 reads in 254.732 CPU sec, 8.143 real sec [M::mem_process_seqs] Processed 697556 reads in 143.188 CPU sec, 4.650 real sec sorting block 131, #reads = 1077212 mem = 536879162 peak = 1110528000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 289630, 11, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 280, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (228.55, 77.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 237, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (263.44, 129.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 155, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2966) [M::mem_pestat] mean and std.dev: (229.78, 309.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 1179, 2842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8122) [M::mem_pestat] mean and std.dev: (1485.27, 1275.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693964 sequences (100000253 bp) in 2.686 CPU sec, 3.132 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 289054, 7, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 344, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1711) [M::mem_pestat] mean and std.dev: (312.14, 188.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 241, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (267.66, 130.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (104, 175, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (176.38, 86.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693806 sequences (100000256 bp) in 2.155 CPU sec, 2.157 real sec... [M::mem_process_seqs] Processed 696752 reads in 212.312 CPU sec, 6.897 real sec [M::mem_process_seqs] Processed 695114 reads in 134.172 CPU sec, 4.324 real sec sorting block 132, #reads = 1079825 mem = 536880918 peak = 1110528000 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289032, 6, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (272.38, 133.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 303, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (339.56, 198.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation RR [M::process] read 693028 sequences (100000108 bp) in 2.791 CPU sec, 2.940 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289033, 10, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 255, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (258.78, 159.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 247, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (273.59, 133.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 6374, 6374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18630) [M::mem_pestat] mean and std.dev: (3458.30, 3215.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24758) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 691962 sequences (100000171 bp) in 2.130 CPU sec, 2.134 real sec... [M::mem_process_seqs] Processed 693964 reads in 202.717 CPU sec, 6.525 real sec sorting block 133, #reads = 1080925 mem = 536878980 peak = 1110528000 [M::mem_process_seqs] Processed 693806 reads in 132.303 CPU sec, 4.209 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 288633, 7, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 398, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4206) [M::mem_pestat] mean and std.dev: (805.00, 614.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (277.17, 136.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (275, 419, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2516) [M::mem_pestat] mean and std.dev: (495.42, 338.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 134, #reads = 1079427 mem = 536878786 peak = 1115103232 [M::process] read 691240 sequences (100000210 bp) in 3.033 CPU sec, 3.334 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 288315, 8, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 242, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (302.37, 158.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (279.91, 136.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 324, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (306.08, 161.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692132 sequences (100000087 bp) in 2.077 CPU sec, 2.094 real sec... [M::mem_process_seqs] Processed 693028 reads in 211.545 CPU sec, 6.786 real sec [M::mem_process_seqs] Processed 691962 reads in 138.664 CPU sec, 4.483 real sec sorting block 135, #reads = 1080855 mem = 536879484 peak = 1115103232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 288124, 6, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1307, 3218, 3381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7529) [M::mem_pestat] mean and std.dev: (2321.92, 1262.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 257, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (283.58, 138.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 223, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2792) [M::mem_pestat] mean and std.dev: (549.29, 647.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3651) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690528 sequences (100000086 bp) in 3.136 CPU sec, 3.365 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 288162, 4, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 309, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (282.83, 105.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (278.05, 136.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 303, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (336.39, 202.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689998 sequences (100000220 bp) in 2.199 CPU sec, 2.218 real sec... [M::mem_process_seqs] Processed 691240 reads in 215.238 CPU sec, 6.956 real sec [M::mem_process_seqs] Processed 692132 reads in 136.698 CPU sec, 4.404 real sec sorting block 136, #reads = 1078814 mem = 536878000 peak = 1115103232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287616, 7, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 260, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (285.95, 139.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 322, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531) [M::mem_pestat] mean and std.dev: (433.17, 284.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1981) [M::mem_pestat] skip orientation RR [M::process] read 689984 sequences (100000225 bp) in 3.227 CPU sec, 3.560 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287286, 2, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 221, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (277.08, 164.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 262, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (287.66, 140.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 429, 1782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4918) [M::mem_pestat] mean and std.dev: (814.13, 746.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6486) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690528 reads in 206.745 CPU sec, 6.666 real sec [M::process] read 689930 sequences (100000105 bp) in 2.205 CPU sec, 2.254 real sec... sorting block 137, #reads = 1080629 mem = 536883433 peak = 1115103232 [M::mem_process_seqs] Processed 689998 reads in 134.360 CPU sec, 4.261 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 287785, 11, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 2333, 2750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7786) [M::mem_pestat] mean and std.dev: (1447.17, 1158.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 261, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (287.04, 140.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1238, 6265, 6265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16319) [M::mem_pestat] mean and std.dev: (4112.27, 2865.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 211, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 367) [M::mem_pestat] mean and std.dev: (212.12, 61.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 138, #reads = 1080840 mem = 536884415 peak = 1115103232 [M::process] read 689658 sequences (100000008 bp) in 2.813 CPU sec, 3.127 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287252, 7, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 327, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (250.67, 87.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (285.80, 139.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 392, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2885) [M::mem_pestat] mean and std.dev: (583.18, 468.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689512 sequences (100000157 bp) in 2.107 CPU sec, 2.122 real sec... [M::mem_process_seqs] Processed 689984 reads in 212.892 CPU sec, 7.058 real sec [M::mem_process_seqs] Processed 689930 reads in 135.357 CPU sec, 4.377 real sec sorting block 139, #reads = 1081120 mem = 536887016 peak = 1115103232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287831, 6, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 260, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (284.23, 137.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::process] read 689524 sequences (100000211 bp) in 3.217 CPU sec, 3.504 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 287071, 4, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 569, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2647) [M::mem_pestat] mean and std.dev: (476.38, 370.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 261, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (286.44, 139.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (302, 484, 1913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5135) [M::mem_pestat] mean and std.dev: (971.07, 774.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689658 reads in 200.928 CPU sec, 6.498 real sec [M::process] read 688818 sequences (100000272 bp) in 2.161 CPU sec, 2.171 real sec... [M::mem_process_seqs] Processed 689512 reads in 130.512 CPU sec, 4.381 real sec sorting block 140, #reads = 1080404 mem = 536881517 peak = 1115103232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287242, 6, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 231, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (206.67, 48.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 260, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (285.81, 138.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 298, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348) [M::mem_pestat] mean and std.dev: (373.42, 284.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688930 sequences (100000050 bp) in 3.008 CPU sec, 3.206 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286650, 6, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 229, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110) [M::mem_pestat] mean and std.dev: (284.10, 174.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 261, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (285.60, 137.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 688496 sequences (100000298 bp) in 1.961 CPU sec, 1.968 real sec... [M::mem_process_seqs] Processed 689524 reads in 195.408 CPU sec, 6.476 real sec [M::mem_process_seqs] Processed 688818 reads in 119.733 CPU sec, 3.782 real sec sorting block 141, #reads = 1081672 mem = 536878069 peak = 1115103232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286807, 3, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 239, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (114, 354) [M::mem_pestat] mean and std.dev: (214.27, 38.68) [M::mem_pestat] low and high boundaries for proper pairs: (60, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 260, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (285.69, 137.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 688424 sequences (100000177 bp) in 3.108 CPU sec, 3.255 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287035, 9, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 320, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (242.73, 78.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 263, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (288.49, 139.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 688930 reads in 182.852 CPU sec, 5.863 real sec [M::process] read 688670 sequences (100000124 bp) in 2.034 CPU sec, 2.039 real sec... sorting block 142, #reads = 1081292 mem = 536879161 peak = 1115103232 [M::mem_process_seqs] Processed 688496 reads in 115.459 CPU sec, 3.643 real sec sorting block 143, #reads = 1081584 mem = 536877712 peak = 1115103232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 286659, 5, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 236, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (269.94, 183.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 263, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (287.89, 139.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (269.75, 160.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688338 sequences (100000204 bp) in 2.817 CPU sec, 2.978 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287032, 2, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 263, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (288.94, 140.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::process] read 688372 sequences (100000225 bp) in 1.821 CPU sec, 1.830 real sec... [M::mem_process_seqs] Processed 688424 reads in 178.085 CPU sec, 5.871 real sec [M::mem_process_seqs] Processed 688670 reads in 105.763 CPU sec, 3.349 real sec sorting block 144, #reads = 1081334 mem = 536883548 peak = 1115103232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 286945, 7, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 337, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (276.10, 120.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 265, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (290.17, 141.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 351, 4424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12892) [M::mem_pestat] mean and std.dev: (1532.90, 2177.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688124 sequences (100000109 bp) in 2.339 CPU sec, 2.484 real sec... [M::process] read 205898 sequences (29790939 bp) in 0.680 CPU sec, 0.680 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286390, 4, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (189.20, 76.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 265, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (290.62, 141.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 276, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (264.67, 108.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688338 reads in 143.596 CPU sec, 4.586 real sec [M::mem_process_seqs] Processed 688372 reads in 91.257 CPU sec, 2.908 real sec sorting block 145, #reads = 1081661 mem = 536879166 peak = 1115103232 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287041, 4, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 335, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (246.33, 107.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 266, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (291.50, 142.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 85631, 2, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 256, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (281.83, 137.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 688124 reads in 99.629 CPU sec, 3.231 real sec [M::mem_process_seqs] Processed 205898 reads in 41.873 CPU sec, 1.351 real sec sorting block 146, #reads = 1082494 mem = 536878058 peak = 1120993280 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 159123696 overall: 69941911552 mem 25170.747 user 624.849 sys 869.146 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_bgw-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:659_bgw-T1-TDNA-1_A23MHWWLT4_1\tLB:659_bgw-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_bgw-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_bgw-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 869.181 sec; CPU: 25795.600 sec sorting block 147, #reads = 640802 mem = 318614430 peak = 1120993280 uniq header count 1 merging 148 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 8672691438 output reads: 159952075 bam_mem_sort: 148 calls 102.952 user 3.432 sys 110.133 real bam_write: 148 calls 91.475 user 6.442 sys 192.160 real execute: 1 calls 46.403 user 1.183 sys 921.460 real merge_files: 1 calls 46.383 user 1.154 sys 51.913 real parse_chunk: 2362 calls 107.666 user 30.762 sys 180.307 real read_chunk: 6375 calls 54.923 user 37.232 sys 805.663 real sort_block: 1 calls 37.710 user 36.553 sys 782.000 real write_chunk: 4011 calls 45.997 user 0.898 sys 50.998 real overall: 3394711552 mem 2125.759 user 95.802 sys 921.481 real ls: cannot access '*.cram': No such file or directory