This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cAd-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1906.704 CPU sec, 66.447 real sec
[M::process] read 684580 sequences (100000067 bp) in 0.968 CPU sec, 0.976 real sec...
[M::process] read 684712 sequences (100000154 bp) in 1.380 CPU sec, 1.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288082, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (304.56, 141.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684448 sequences (100000158 bp) in 1.501 CPU sec, 1.510 real sec...
[M::process] read 684318 sequences (100000041 bp) in 1.507 CPU sec, 1.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287072, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (305.50, 140.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684580 reads in 176.561 CPU sec, 5.624 real sec
[M::mem_process_seqs] Processed 684712 reads in 102.040 CPU sec, 3.306 real sec
sorting block 0, #reads = 1087696 mem = 536881265 peak = 573063168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287375, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (305.41, 139.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683848 sequences (100000199 bp) in 1.999 CPU sec, 2.118 real sec...
[M::process] read 683798 sequences (100000090 bp) in 1.500 CPU sec, 1.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287402, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (306.73, 141.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684448 reads in 154.051 CPU sec, 4.916 real sec
[M::mem_process_seqs] Processed 684318 reads in 99.442 CPU sec, 3.183 real sec
sorting block 1, #reads = 1087608 mem = 536882491 peak = 690806784
[M::process] read 683294 sequences (100000127 bp) in 1.912 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287249, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (705, 705, 705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (705, 705)
[M::mem_pestat] mean and std.dev: (705.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (705, 705)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (309.86, 143.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (366, 985, 985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2223)
[M::mem_pestat] mean and std.dev: (700.18, 345.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2842)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685084 sequences (100000000 bp) in 1.600 CPU sec, 1.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287251, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (309.94, 142.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287270, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (314.32, 144.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683848 reads in 224.644 CPU sec, 7.261 real sec
[M::mem_process_seqs] Processed 683798 reads in 169.309 CPU sec, 5.513 real sec
sorting block 2, #reads = 1086816 mem = 536881042 peak = 827682816
[M::mem_process_seqs] Processed 683294 reads in 184.603 CPU sec, 6.016 real sec
sorting block 3, #reads = 1086301 mem = 536878489 peak = 827682816
[M::process] read 682516 sequences (100000133 bp) in 2.455 CPU sec, 2.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287579, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (305.77, 143.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682328 sequences (100000266 bp) in 1.887 CPU sec, 1.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287092, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (319.08, 146.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685084 reads in 160.022 CPU sec, 5.099 real sec
[M::mem_process_seqs] Processed 682516 reads in 101.289 CPU sec, 3.189 real sec
[M::process] read 681876 sequences (100000017 bp) in 1.698 CPU sec, 1.748 real sec...
sorting block 4, #reads = 1087167 mem = 536878441 peak = 827682816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286892, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 306, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (320.72, 147.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681236 sequences (100000254 bp) in 2.087 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287060, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 681494 sequences (100000298 bp) in 1.635 CPU sec, 1.651 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (322.57, 147.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682328 reads in 155.827 CPU sec, 4.939 real sec
sorting block 5, #reads = 1086642 mem = 536878121 peak = 827682816
[M::mem_process_seqs] Processed 681876 reads in 100.459 CPU sec, 3.181 real sec
[M::process] read 681126 sequences (100000017 bp) in 2.205 CPU sec, 2.315 real sec...
[M::process] read 681972 sequences (100000155 bp) in 1.772 CPU sec, 1.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286338, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (324.37, 147.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681126 reads in 82.867 CPU sec, 2.736 real sec
sorting block 6, #reads = 1085664 mem = 536878063 peak = 827682816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286967, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 308, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (322.03, 146.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681494 reads in 170.937 CPU sec, 6.178 real sec
sorting block 7, #reads = 1084698 mem = 536879373 peak = 918581248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286447, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (323.56, 147.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681116 sequences (100000142 bp) in 1.725 CPU sec, 1.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287199, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (317.76, 145.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680322 sequences (100000226 bp) in 1.268 CPU sec, 1.280 real sec...
[M::mem_process_seqs] Processed 681236 reads in 327.804 CPU sec, 11.160 real sec
[M::mem_process_seqs] Processed 681972 reads in 118.863 CPU sec, 3.777 real sec
sorting block 8, #reads = 1085512 mem = 536881300 peak = 918581248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286257, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (320.61, 145.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680568 sequences (100000115 bp) in 2.101 CPU sec, 2.260 real sec...
[M::process] read 680026 sequences (100000005 bp) in 1.307 CPU sec, 1.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286173, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 311, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (324.69, 145.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681116 reads in 154.261 CPU sec, 4.925 real sec
[M::mem_process_seqs] Processed 680322 reads in 98.768 CPU sec, 3.152 real sec
sorting block 9, #reads = 1085818 mem = 536878780 peak = 918581248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286069, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (325.65, 147.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679910 sequences (100000261 bp) in 2.286 CPU sec, 2.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286137, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (326.62, 146.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679824 sequences (100000232 bp) in 1.740 CPU sec, 1.734 real sec...
[M::mem_process_seqs] Processed 680568 reads in 154.821 CPU sec, 4.930 real sec
sorting block 10, #reads = 1084895 mem = 536880037 peak = 918581248
[M::mem_process_seqs] Processed 680026 reads in 99.483 CPU sec, 3.152 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286413, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 313, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (325.88, 145.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1662, 8156, 8156)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21144)
[M::mem_pestat] mean and std.dev: (4684.30, 3502.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 27638)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 680334 sequences (100000218 bp) in 2.585 CPU sec, 2.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285989, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (325.43, 145.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679910 reads in 147.038 CPU sec, 4.657 real sec
sorting block 11, #reads = 1084286 mem = 536882525 peak = 918581248
[M::process] read 680260 sequences (100000270 bp) in 1.821 CPU sec, 1.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286302, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 310, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (323.43, 144.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680438 sequences (100000120 bp) in 1.832 CPU sec, 1.841 real sec...
[M::mem_process_seqs] Processed 680334 reads in 88.448 CPU sec, 2.975 real sec
sorting block 12, #reads = 1084792 mem = 536879581 peak = 944930816
[M::mem_process_seqs] Processed 679824 reads in 209.880 CPU sec, 6.816 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285729, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (322.52, 144.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680570 sequences (100000032 bp) in 2.621 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286316, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 309, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (322.08, 143.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680260 reads in 167.482 CPU sec, 5.324 real sec
sorting block 13, #reads = 1085091 mem = 536882709 peak = 944930816
[M::mem_process_seqs] Processed 680438 reads in 99.813 CPU sec, 3.152 real sec
[M::process] read 680096 sequences (100000211 bp) in 2.106 CPU sec, 2.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286270, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 308, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (320.88, 143.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1085375 mem = 536879259 peak = 944930816
[M::process] read 679804 sequences (100000022 bp) in 3.092 CPU sec, 3.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286235, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 311, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (324.22, 144.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680570 reads in 148.967 CPU sec, 4.752 real sec
[M::mem_process_seqs] Processed 680096 reads in 98.160 CPU sec, 3.099 real sec
[M::process] read 679702 sequences (100000143 bp) in 2.297 CPU sec, 2.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285740, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 312, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (324.82, 144.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1085820 mem = 536879311 peak = 944930816
[M::process] read 679508 sequences (100000077 bp) in 2.194 CPU sec, 2.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286377, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 312, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (324.74, 144.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679804 reads in 151.987 CPU sec, 4.841 real sec
[M::mem_process_seqs] Processed 679702 reads in 100.906 CPU sec, 3.185 real sec
[M::process] read 679800 sequences (100000029 bp) in 2.227 CPU sec, 2.311 real sec...
sorting block 16, #reads = 1084855 mem = 536878976 peak = 944930816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285524, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 268, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (363.30, 298.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1558)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 315, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (327.11, 145.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684784 sequences (100000106 bp) in 2.514 CPU sec, 2.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286146, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (327.00, 146.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679508 reads in 152.676 CPU sec, 4.861 real sec
[M::mem_process_seqs] Processed 679800 reads in 103.237 CPU sec, 3.247 real sec
[M::process] read 684602 sequences (100000269 bp) in 2.025 CPU sec, 2.093 real sec...
sorting block 17, #reads = 1085102 mem = 536880077 peak = 944930816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287741, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (306.97, 143.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684144 sequences (100000012 bp) in 2.515 CPU sec, 2.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287029, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (307.86, 142.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684784 reads in 151.634 CPU sec, 4.823 real sec
sorting block 18, #reads = 1084635 mem = 536879081 peak = 944930816
[M::mem_process_seqs] Processed 684602 reads in 98.234 CPU sec, 3.092 real sec
[M::process] read 683970 sequences (100000127 bp) in 1.871 CPU sec, 1.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287243, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (310.36, 143.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1087519 mem = 536877843 peak = 944930816
[M::process] read 683960 sequences (100000148 bp) in 2.356 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287581, 8, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 323, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (281.70, 101.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (311.02, 143.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 684144 reads in 148.922 CPU sec, 4.752 real sec
[M::process] read 683462 sequences (100000069 bp) in 1.725 CPU sec, 1.759 real sec...
[M::mem_process_seqs] Processed 683970 reads in 97.270 CPU sec, 3.067 real sec
sorting block 20, #reads = 1087225 mem = 536879135 peak = 944930816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287665, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (311.26, 143.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 493, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (386.92, 153.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RR
[M::process] read 683428 sequences (100000234 bp) in 2.494 CPU sec, 2.616 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287143, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (313.66, 144.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683960 reads in 147.924 CPU sec, 4.700 real sec
[M::process] read 682788 sequences (100000100 bp) in 1.846 CPU sec, 1.867 real sec...
[M::mem_process_seqs] Processed 683462 reads in 97.160 CPU sec, 3.057 real sec
sorting block 21, #reads = 1088812 mem = 536878494 peak = 944930816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287428, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 300, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (315.39, 145.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 265, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (249, 279)
[M::mem_pestat] mean and std.dev: (265.00, 2.31)
[M::mem_pestat] low and high boundaries for proper pairs: (243, 285)
[M::mem_pestat] skip orientation RR
[M::process] read 682524 sequences (100000100 bp) in 2.221 CPU sec, 2.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286764, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (318.19, 147.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682088 sequences (100000119 bp) in 1.749 CPU sec, 1.752 real sec...
[M::mem_process_seqs] Processed 683428 reads in 148.457 CPU sec, 4.713 real sec
[M::mem_process_seqs] Processed 682788 reads in 95.096 CPU sec, 3.006 real sec
sorting block 22, #reads = 1086893 mem = 536877265 peak = 944930816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286974, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (305, 1768, 1768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4694)
[M::mem_pestat] mean and std.dev: (1235.60, 759.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6157)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 306, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (321.15, 148.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681526 sequences (100000029 bp) in 2.627 CPU sec, 2.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286869, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (322.99, 148.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682524 reads in 147.411 CPU sec, 4.674 real sec
sorting block 23, #reads = 1087249 mem = 536879903 peak = 944930816
[M::process] read 681380 sequences (100000205 bp) in 1.930 CPU sec, 1.958 real sec...
[M::mem_process_seqs] Processed 682088 reads in 97.447 CPU sec, 3.066 real sec
sorting block 24, #reads = 1085843 mem = 536878100 peak = 987226112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286372, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 311, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (325.45, 149.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681358 sequences (100000092 bp) in 2.202 CPU sec, 2.306 real sec...
[M::process] read 681150 sequences (100000247 bp) in 1.427 CPU sec, 1.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286289, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 311, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (325.82, 149.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681526 reads in 148.357 CPU sec, 4.724 real sec
[M::mem_process_seqs] Processed 681380 reads in 96.067 CPU sec, 3.044 real sec
sorting block 25, #reads = 1086185 mem = 536880068 peak = 987226112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287120, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 312, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (325.86, 149.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680792 sequences (100000113 bp) in 2.320 CPU sec, 2.443 real sec...
[M::process] read 680572 sequences (100000014 bp) in 1.424 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286692, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 314, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (326.66, 147.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681358 reads in 149.773 CPU sec, 4.764 real sec
sorting block 26, #reads = 1085455 mem = 536879431 peak = 987226112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286680, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 314, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (325.75, 146.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680256 sequences (100000257 bp) in 1.962 CPU sec, 1.967 real sec...
[M::mem_process_seqs] Processed 680792 reads in 81.095 CPU sec, 2.561 real sec
[M::mem_process_seqs] Processed 681150 reads in 202.321 CPU sec, 6.427 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285964, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (326.33, 147.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1086355 mem = 536878507 peak = 987226112
[M::process] read 680108 sequences (100000224 bp) in 2.339 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286292, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (327.24, 146.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680572 reads in 163.295 CPU sec, 5.188 real sec
[M::process] read 680202 sequences (100000226 bp) in 1.955 CPU sec, 2.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286358, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 314, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (327.33, 146.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680192 sequences (100000016 bp) in 2.020 CPU sec, 2.027 real sec...
[M::mem_process_seqs] Processed 680108 reads in 85.413 CPU sec, 2.701 real sec
sorting block 28, #reads = 1085052 mem = 536880010 peak = 987226112
[M::mem_process_seqs] Processed 680256 reads in 216.106 CPU sec, 6.855 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286192, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (326.86, 146.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1084876 mem = 536884992 peak = 1023123456
[M::process] read 679826 sequences (100000238 bp) in 2.748 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285976, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 316, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (328.29, 147.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680202 reads in 164.632 CPU sec, 5.238 real sec
[M::mem_process_seqs] Processed 680192 reads in 99.188 CPU sec, 3.132 real sec
[M::process] read 679806 sequences (100000232 bp) in 2.314 CPU sec, 2.454 real sec...
sorting block 30, #reads = 1085449 mem = 536879898 peak = 1023123456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286332, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 313, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (325.76, 144.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680448 sequences (100000269 bp) in 2.088 CPU sec, 2.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286106, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 311, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (323.87, 143.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679826 reads in 148.662 CPU sec, 4.720 real sec
[M::mem_process_seqs] Processed 679806 reads in 96.256 CPU sec, 3.025 real sec
[M::process] read 680448 sequences (100000190 bp) in 2.179 CPU sec, 2.235 real sec...
sorting block 31, #reads = 1084655 mem = 536879242 peak = 1023123456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286695, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 309, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (322.49, 143.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680286 sequences (100000284 bp) in 2.530 CPU sec, 2.707 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287001, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (322.86, 144.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680448 reads in 148.854 CPU sec, 4.769 real sec
[M::mem_process_seqs] Processed 680448 reads in 98.204 CPU sec, 3.098 real sec
sorting block 32, #reads = 1083908 mem = 536878431 peak = 1023123456
[M::process] read 680602 sequences (100000253 bp) in 2.322 CPU sec, 2.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286322, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 310, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (323.24, 144.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1084952 mem = 536880450 peak = 1023123456
[M::process] read 680036 sequences (100000091 bp) in 2.189 CPU sec, 2.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286513, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 309, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (322.01, 143.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680286 reads in 155.826 CPU sec, 4.967 real sec
[M::mem_process_seqs] Processed 680602 reads in 102.189 CPU sec, 3.222 real sec
[M::process] read 680236 sequences (100000018 bp) in 1.887 CPU sec, 1.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286206, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 310, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (323.10, 144.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1085563 mem = 536877568 peak = 1023123456
[M::process] read 679972 sequences (100000300 bp) in 2.707 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286454, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 310, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (322.84, 144.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680036 reads in 154.475 CPU sec, 4.921 real sec
[M::mem_process_seqs] Processed 680236 reads in 101.048 CPU sec, 3.180 real sec
[M::process] read 680254 sequences (100000074 bp) in 2.001 CPU sec, 2.055 real sec...
sorting block 35, #reads = 1084705 mem = 536878606 peak = 1023123456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286159, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (325.80, 146.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685382 sequences (100000173 bp) in 2.367 CPU sec, 2.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286360, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 313, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (326.41, 146.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679972 reads in 152.142 CPU sec, 4.835 real sec
[M::process] read 684838 sequences (100000129 bp) in 1.885 CPU sec, 1.912 real sec...
[M::mem_process_seqs] Processed 680254 reads in 99.257 CPU sec, 3.123 real sec
sorting block 36, #reads = 1084527 mem = 536881087 peak = 1023123456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287671, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (304.80, 142.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684274 sequences (100000200 bp) in 2.508 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288027, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (307.01, 143.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685382 reads in 158.588 CPU sec, 5.040 real sec
[M::process] read 683868 sequences (100000154 bp) in 1.837 CPU sec, 1.843 real sec...
sorting block 37, #reads = 1083869 mem = 536879594 peak = 1023123456
[M::mem_process_seqs] Processed 684838 reads in 104.517 CPU sec, 3.298 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287704, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (308.76, 142.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1088043 mem = 536882527 peak = 1023123456
[M::process] read 683648 sequences (100000022 bp) in 2.440 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287734, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (310.63, 142.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684274 reads in 156.055 CPU sec, 4.971 real sec
[M::process] read 683578 sequences (100000091 bp) in 1.997 CPU sec, 2.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287321, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (312.21, 143.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683134 sequences (100000124 bp) in 2.000 CPU sec, 2.006 real sec...
[M::mem_process_seqs] Processed 683648 reads in 91.681 CPU sec, 3.044 real sec
sorting block 39, #reads = 1086769 mem = 536878585 peak = 1023123456
[M::mem_process_seqs] Processed 683868 reads in 231.675 CPU sec, 7.493 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287301, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (313.22, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682590 sequences (100000205 bp) in 2.420 CPU sec, 2.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286967, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (316.11, 145.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683578 reads in 173.987 CPU sec, 5.550 real sec
sorting block 40, #reads = 1085860 mem = 536879972 peak = 1023123456
[M::mem_process_seqs] Processed 683134 reads in 104.940 CPU sec, 3.343 real sec
[M::process] read 682370 sequences (100000008 bp) in 1.946 CPU sec, 2.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287660, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (318.57, 145.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1086107 mem = 536878785 peak = 1027923968
[M::process] read 682060 sequences (100000232 bp) in 2.702 CPU sec, 2.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287521, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (320.87, 147.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682590 reads in 159.393 CPU sec, 5.143 real sec
[M::mem_process_seqs] Processed 682370 reads in 105.159 CPU sec, 3.349 real sec
[M::process] read 681790 sequences (100000154 bp) in 2.303 CPU sec, 2.462 real sec...
sorting block 42, #reads = 1086042 mem = 536881179 peak = 1027923968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287275, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (322.56, 148.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681418 sequences (100000082 bp) in 2.381 CPU sec, 2.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287049, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 310, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (324.78, 149.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682060 reads in 160.374 CPU sec, 5.156 real sec
[M::mem_process_seqs] Processed 681790 reads in 106.148 CPU sec, 3.375 real sec
[M::process] read 681324 sequences (100000176 bp) in 2.006 CPU sec, 2.238 real sec...
sorting block 43, #reads = 1086349 mem = 536876924 peak = 1027923968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286615, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (324.85, 148.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681322 sequences (100000187 bp) in 2.672 CPU sec, 3.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286974, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 311, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (325.14, 148.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 827, 913)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2349)
[M::mem_pestat] mean and std.dev: (793.90, 532.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3067)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681418 reads in 158.329 CPU sec, 5.169 real sec
[M::mem_process_seqs] Processed 681324 reads in 105.274 CPU sec, 3.520 real sec
sorting block 44, #reads = 1085354 mem = 536879719 peak = 1027923968
[M::process] read 680814 sequences (100000208 bp) in 1.982 CPU sec, 2.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286818, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 312, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (326.32, 149.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681194 sequences (100000190 bp) in 2.152 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286287, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 312, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (326.26, 148.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1084809 mem = 536881896 peak = 1027923968
[M::mem_process_seqs] Processed 681322 reads in 159.941 CPU sec, 5.464 real sec
[M::mem_process_seqs] Processed 680814 reads in 105.510 CPU sec, 3.661 real sec
[M::process] read 681250 sequences (100000080 bp) in 2.069 CPU sec, 2.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286725, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (325.48, 148.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1085785 mem = 536881741 peak = 1027923968
[M::process] read 681366 sequences (100000116 bp) in 2.335 CPU sec, 2.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286757, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 308, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (322.86, 147.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681194 reads in 160.083 CPU sec, 5.342 real sec
[M::mem_process_seqs] Processed 681250 reads in 107.352 CPU sec, 3.628 real sec
[M::process] read 681142 sequences (100000295 bp) in 2.215 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286831, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 308, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (323.63, 148.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1084634 mem = 536879961 peak = 1027923968
[M::process] read 681430 sequences (100000065 bp) in 2.273 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286269, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (322.29, 147.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681366 reads in 163.829 CPU sec, 5.501 real sec
[M::mem_process_seqs] Processed 681142 reads in 109.326 CPU sec, 3.584 real sec
[M::process] read 681116 sequences (100000080 bp) in 2.469 CPU sec, 2.666 real sec...
sorting block 48, #reads = 1085824 mem = 536880726 peak = 1032114176
[M::process] read 681322 sequences (100000246 bp) in 2.501 CPU sec, 2.781 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286570, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 305, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (319.43, 144.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681116 reads in 92.294 CPU sec, 2.962 real sec
sorting block 49, #reads = 1085769 mem = 536880595 peak = 1032114176
[M::process] read 680988 sequences (100000005 bp) in 2.095 CPU sec, 2.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286630, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 305, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (319.56, 145.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 287357, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (327, 587, 940)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166)
[M::mem_pestat] mean and std.dev: (587.94, 419.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2779)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 306, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (321.10, 146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 347, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (287.40, 145.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681430 reads in 254.673 CPU sec, 8.282 real sec
[M::mem_process_seqs] Processed 681322 reads in 124.328 CPU sec, 4.060 real sec
[M::process] read 680570 sequences (100000181 bp) in 2.530 CPU sec, 2.830 real sec...
sorting block 50, #reads = 1084836 mem = 536877389 peak = 1032114176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286193, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (320.43, 144.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680674 sequences (100000208 bp) in 2.197 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286571, 14, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 393, 897)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2457)
[M::mem_pestat] mean and std.dev: (507.15, 376.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3237)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (321.67, 144.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 2567, 2636)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7752)
[M::mem_pestat] mean and std.dev: (2203.93, 1823.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10310)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 680988 reads in 166.072 CPU sec, 5.431 real sec
[M::mem_process_seqs] Processed 680570 reads in 109.092 CPU sec, 3.519 real sec
sorting block 51, #reads = 1085419 mem = 536885391 peak = 1032114176
[M::process] read 680274 sequences (100000024 bp) in 2.144 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286830, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (321.82, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680484 sequences (100000097 bp) in 2.504 CPU sec, 2.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287231, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (324.60, 146.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680674 reads in 158.952 CPU sec, 5.235 real sec
sorting block 52, #reads = 1083578 mem = 536884348 peak = 1032114176
[M::mem_process_seqs] Processed 680274 reads in 104.222 CPU sec, 3.385 real sec
[M::process] read 680212 sequences (100000249 bp) in 2.426 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286385, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (349, 453, 751)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1555)
[M::mem_pestat] mean and std.dev: (412.12, 225.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1957)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (323.62, 145.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 53, #reads = 1085485 mem = 536880069 peak = 1071644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286574, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 309, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (322.82, 144.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679934 sequences (100000078 bp) in 2.459 CPU sec, 2.907 real sec...
[M::mem_process_seqs] Processed 680484 reads in 156.591 CPU sec, 5.084 real sec
[M::mem_process_seqs] Processed 680212 reads in 102.387 CPU sec, 3.298 real sec
[M::process] read 682558 sequences (100000158 bp) in 2.439 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286595, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 311, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (324.51, 145.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1085466 mem = 536879414 peak = 1071644672
[M::process] read 684722 sequences (100000093 bp) in 2.325 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286765, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (316.83, 145.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679934 reads in 162.071 CPU sec, 5.215 real sec
[M::mem_process_seqs] Processed 682558 reads in 110.138 CPU sec, 3.499 real sec
[M::process] read 684308 sequences (100000017 bp) in 2.348 CPU sec, 2.466 real sec...
sorting block 55, #reads = 1085703 mem = 536879548 peak = 1071644672
[M::process] read 683834 sequences (100000182 bp) in 2.420 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287417, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (285, 285, 1117)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2781)
[M::mem_pestat] mean and std.dev: (547.70, 378.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3613)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (310.92, 145.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 684308 reads in 88.052 CPU sec, 2.790 real sec
sorting block 56, #reads = 1085208 mem = 536885548 peak = 1071644672
[M::process] read 683346 sequences (100000105 bp) in 2.175 CPU sec, 2.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287665, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (308.63, 144.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287411, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 231, 1973)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5593)
[M::mem_pestat] mean and std.dev: (781.90, 797.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7403)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (313.10, 146.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 684722 reads in 244.558 CPU sec, 7.853 real sec
[M::mem_process_seqs] Processed 683834 reads in 121.696 CPU sec, 3.882 real sec
[M::process] read 683400 sequences (100000141 bp) in 2.612 CPU sec, 2.774 real sec...
sorting block 57, #reads = 1089050 mem = 536880843 peak = 1071644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287184, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (315.59, 146.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683126 sequences (100000132 bp) in 2.469 CPU sec, 2.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287143, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 300, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (316.06, 147.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (528, 528, 861)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1527)
[M::mem_pestat] mean and std.dev: (537.88, 224.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1860)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683346 reads in 153.939 CPU sec, 4.943 real sec
[M::mem_process_seqs] Processed 683400 reads in 102.300 CPU sec, 3.254 real sec
sorting block 58, #reads = 1087506 mem = 536880741 peak = 1071644672
[M::process] read 682510 sequences (100000260 bp) in 2.256 CPU sec, 2.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287454, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 268, 938)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2440)
[M::mem_pestat] mean and std.dev: (473.80, 335.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3191)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (317.27, 147.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682290 sequences (100000300 bp) in 2.441 CPU sec, 2.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287039, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (318.93, 146.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 266, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (318.60, 120.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683126 reads in 152.207 CPU sec, 4.898 real sec
[M::mem_process_seqs] Processed 682510 reads in 101.885 CPU sec, 3.235 real sec
sorting block 59, #reads = 1088020 mem = 536879571 peak = 1071644672
[M::process] read 682056 sequences (100000232 bp) in 2.587 CPU sec, 2.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286996, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 588, 2952)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8372)
[M::mem_pestat] mean and std.dev: (1073.79, 1197.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11082)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (320.69, 148.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 60, #reads = 1087573 mem = 536878656 peak = 1071644672
[M::process] read 681918 sequences (100000169 bp) in 2.584 CPU sec, 2.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286570, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (322.44, 148.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 605, 803)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1945)
[M::mem_pestat] mean and std.dev: (502.55, 261.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2516)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682290 reads in 159.308 CPU sec, 5.109 real sec
[M::mem_process_seqs] Processed 682056 reads in 103.994 CPU sec, 3.286 real sec
[M::process] read 681468 sequences (100000020 bp) in 2.337 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287288, 11, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (323.36, 149.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 37, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1918)
[M::mem_pestat] mean and std.dev: (187.30, 247.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2545)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 61, #reads = 1087049 mem = 536876583 peak = 1071644672
[M::process] read 681598 sequences (100000055 bp) in 2.565 CPU sec, 2.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286904, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 311, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (326.19, 150.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681918 reads in 160.114 CPU sec, 5.123 real sec
[M::mem_process_seqs] Processed 681468 reads in 102.862 CPU sec, 3.249 real sec
[M::process] read 681466 sequences (100000112 bp) in 2.259 CPU sec, 2.360 real sec...
sorting block 62, #reads = 1086883 mem = 536883732 peak = 1071644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286625, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (325.40, 150.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681402 sequences (100000218 bp) in 2.436 CPU sec, 2.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286408, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (325.95, 150.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681598 reads in 154.200 CPU sec, 4.916 real sec
[M::mem_process_seqs] Processed 681466 reads in 101.399 CPU sec, 3.200 real sec
[M::process] read 681396 sequences (100000008 bp) in 1.927 CPU sec, 1.983 real sec...
sorting block 63, #reads = 1086361 mem = 536878477 peak = 1071644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286643, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (326.24, 151.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 511, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729)
[M::mem_pestat] mean and std.dev: (491.73, 267.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2225)
[M::mem_pestat] skip orientation RR
[M::process] read 681838 sequences (100000265 bp) in 2.174 CPU sec, 2.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286316, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 309, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (324.43, 149.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681402 reads in 161.562 CPU sec, 5.327 real sec
sorting block 64, #reads = 1085663 mem = 536876303 peak = 1071644672
[M::mem_process_seqs] Processed 681396 reads in 106.973 CPU sec, 3.539 real sec
[M::process] read 681810 sequences (100000084 bp) in 2.021 CPU sec, 2.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287309, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 437, 1435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3673)
[M::mem_pestat] mean and std.dev: (837.09, 611.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4792)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (321.71, 149.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 65, #reads = 1085776 mem = 536878289 peak = 1071644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286714, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (321.58, 149.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682136 sequences (100000132 bp) in 2.833 CPU sec, 3.365 real sec...
[M::mem_process_seqs] Processed 681838 reads in 164.312 CPU sec, 5.557 real sec
[M::mem_process_seqs] Processed 681810 reads in 109.228 CPU sec, 3.645 real sec
[M::process] read 682186 sequences (100000169 bp) in 2.197 CPU sec, 2.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287417, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (319.22, 147.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682136 reads in 89.873 CPU sec, 3.248 real sec
sorting block 66, #reads = 1087236 mem = 536878071 peak = 1071644672
[M::process] read 681936 sequences (100000023 bp) in 2.341 CPU sec, 2.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287375, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (318.27, 147.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287224, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 302, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (318.47, 146.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682286 sequences (100000156 bp) in 2.147 CPU sec, 2.777 real sec...
sorting block 67, #reads = 1086128 mem = 536878915 peak = 1071644672
[M::mem_process_seqs] Processed 682186 reads in 164.861 CPU sec, 5.467 real sec
[M::mem_process_seqs] Processed 681936 reads in 106.897 CPU sec, 3.529 real sec
[M::process] read 681868 sequences (100000149 bp) in 2.356 CPU sec, 2.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286778, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (318.25, 147.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1087113 mem = 536878497 peak = 1071644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286741, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 301, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (317.19, 146.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681592 sequences (100000040 bp) in 2.516 CPU sec, 2.946 real sec...
[M::mem_process_seqs] Processed 682286 reads in 166.145 CPU sec, 5.464 real sec
[M::mem_process_seqs] Processed 681868 reads in 108.402 CPU sec, 3.556 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286340, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 301, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (316.52, 144.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681422 sequences (100000248 bp) in 2.226 CPU sec, 2.780 real sec...
[M::mem_process_seqs] Processed 681592 reads in 89.372 CPU sec, 3.121 real sec
sorting block 69, #reads = 1085751 mem = 536880312 peak = 1071644672
[M::process] read 681016 sequences (100000274 bp) in 2.452 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286995, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 305, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (319.92, 145.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681422 reads in 92.911 CPU sec, 3.197 real sec
sorting block 70, #reads = 1085662 mem = 536882556 peak = 1071644672
[M::process] read 681030 sequences (100000130 bp) in 2.404 CPU sec, 2.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286339, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 305, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (320.85, 146.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681016 reads in 87.915 CPU sec, 3.043 real sec
[M::process] read 680978 sequences (100000016 bp) in 2.396 CPU sec, 2.833 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286958, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (321.40, 145.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681030 reads in 93.328 CPU sec, 3.234 real sec
sorting block 71, #reads = 1085336 mem = 536880528 peak = 1071644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287132, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (322.95, 147.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680946 sequences (100000038 bp) in 2.426 CPU sec, 2.627 real sec...
[M::mem_process_seqs] Processed 680978 reads in 91.105 CPU sec, 3.231 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286905, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (322.23, 146.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680760 sequences (100000065 bp) in 2.483 CPU sec, 2.783 real sec...
sorting block 72, #reads = 1085464 mem = 536881922 peak = 1071644672
[M::mem_process_seqs] Processed 680946 reads in 88.731 CPU sec, 3.156 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286769, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (322.73, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683990 sequences (100000172 bp) in 2.489 CPU sec, 2.816 real sec...
[M::mem_process_seqs] Processed 680760 reads in 91.827 CPU sec, 3.280 real sec
sorting block 73, #reads = 1085613 mem = 536879757 peak = 1071644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287664, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 293, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (311.71, 146.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 423, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (347.00, 127.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RR
[M::process] read 687988 sequences (100000257 bp) in 2.680 CPU sec, 2.985 real sec...
[M::mem_process_seqs] Processed 683990 reads in 100.501 CPU sec, 3.565 real sec
sorting block 74, #reads = 1085320 mem = 536878621 peak = 1071644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288822, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 469, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (319.00, 152.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (298.63, 145.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688046 sequences (100000015 bp) in 2.487 CPU sec, 2.976 real sec...
[M::mem_process_seqs] Processed 687988 reads in 102.381 CPU sec, 3.507 real sec
[M::process] read 686866 sequences (100000157 bp) in 2.433 CPU sec, 2.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289612, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 274, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (297.08, 144.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 75, #reads = 1084645 mem = 536877981 peak = 1071644672
[M::process] read 686408 sequences (100000187 bp) in 2.565 CPU sec, 2.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288493, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (302.99, 146.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688046 reads in 184.203 CPU sec, 6.112 real sec
[M::mem_process_seqs] Processed 686866 reads in 118.852 CPU sec, 3.876 real sec
[M::process] read 686164 sequences (100000039 bp) in 2.417 CPU sec, 2.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288387, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (304.73, 147.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 76, #reads = 1086542 mem = 536880325 peak = 1071644672
[M::process] read 686680 sequences (100000242 bp) in 2.362 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 288354, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (306.02, 147.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686408 reads in 177.126 CPU sec, 5.899 real sec
[M::mem_process_seqs] Processed 686164 reads in 117.038 CPU sec, 3.941 real sec
sorting block 77, #reads = 1087096 mem = 536885730 peak = 1071644672
[M::process] read 686140 sequences (100000205 bp) in 2.579 CPU sec, 2.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288623, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (301.17, 145.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288353, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 281, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (302.62, 145.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 326, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (44, 514)
[M::mem_pestat] mean and std.dev: (280.54, 66.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] skip orientation RR
[M::process] read 685190 sequences (100000195 bp) in 2.445 CPU sec, 3.235 real sec...
[M::mem_process_seqs] Processed 686680 reads in 178.053 CPU sec, 5.934 real sec
[M::mem_process_seqs] Processed 686140 reads in 117.503 CPU sec, 3.954 real sec
sorting block 78, #reads = 1086695 mem = 536880608 peak = 1071644672
[M::process] read 685086 sequences (100000201 bp) in 2.761 CPU sec, 3.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288104, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (309.74, 148.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287658, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (310.76, 148.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683558 sequences (100000033 bp) in 2.413 CPU sec, 2.881 real sec...
sorting block 79, #reads = 1086847 mem = 536877373 peak = 1071644672
[M::mem_process_seqs] Processed 685190 reads in 176.910 CPU sec, 5.874 real sec
[M::mem_process_seqs] Processed 685086 reads in 114.911 CPU sec, 3.835 real sec
[M::process] read 684534 sequences (100000228 bp) in 2.392 CPU sec, 2.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287392, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 298, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (317.60, 150.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1086099 mem = 536880630 peak = 1071644672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288074, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (315.38, 150.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684262 sequences (100000010 bp) in 2.576 CPU sec, 3.071 real sec...
[M::mem_process_seqs] Processed 683558 reads in 181.635 CPU sec, 6.055 real sec
[M::mem_process_seqs] Processed 684534 reads in 117.154 CPU sec, 3.908 real sec
[M::process] read 685606 sequences (100000114 bp) in 2.659 CPU sec, 3.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287365, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (314.81, 149.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 81, #reads = 1084456 mem = 536880645 peak = 1071644672
[M::mem_process_seqs] Processed 684262 reads in 98.257 CPU sec, 3.413 real sec
[M::process] read 682640 sequences (100000211 bp) in 2.306 CPU sec, 2.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 287980, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 470, 1472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4184)
[M::mem_pestat] mean and std.dev: (828.12, 754.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5540)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (301.61, 143.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 82, #reads = 1085734 mem = 536879459 peak = 1093033984
[M::process] read 683690 sequences (100000120 bp) in 2.393 CPU sec, 2.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287152, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (320.47, 150.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685606 reads in 180.390 CPU sec, 6.019 real sec
[M::mem_process_seqs] Processed 682640 reads in 117.494 CPU sec, 3.950 real sec
[M::process] read 683254 sequences (100000025 bp) in 2.455 CPU sec, 2.878 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287301, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (498, 498, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (498, 498)
[M::mem_pestat] mean and std.dev: (498.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (498, 498)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 300, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (318.15, 150.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 83, #reads = 1085076 mem = 536880266 peak = 1093033984
[M::process] read 682560 sequences (100000249 bp) in 2.664 CPU sec, 3.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287308, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 301, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (319.37, 150.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683690 reads in 178.175 CPU sec, 5.960 real sec
[M::mem_process_seqs] Processed 683254 reads in 117.211 CPU sec, 3.983 real sec
sorting block 84, #reads = 1084212 mem = 536880403 peak = 1093033984
[M::process] read 681834 sequences (100000181 bp) in 2.429 CPU sec, 2.746 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287443, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (322.66, 147.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681248 sequences (100000144 bp) in 2.457 CPU sec, 3.279 real sec...
[M::mem_process_seqs] Processed 681834 reads in 101.174 CPU sec, 3.471 real sec
sorting block 85, #reads = 1083631 mem = 536878247 peak = 1093033984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286737, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 184, 184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (184, 184)
[M::mem_pestat] mean and std.dev: (184.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (184, 184)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (324.79, 148.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681150 sequences (100000076 bp) in 2.378 CPU sec, 2.853 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287260, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (320.78, 148.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681248 reads in 100.048 CPU sec, 3.577 real sec
[M::mem_process_seqs] Processed 682560 reads in 299.613 CPU sec, 10.498 real sec
[M::process] read 680622 sequences (100000232 bp) in 2.123 CPU sec, 2.400 real sec...
sorting block 86, #reads = 1082723 mem = 536878007 peak = 1093033984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287055, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (324.33, 147.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680742 sequences (100000173 bp) in 2.642 CPU sec, 3.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286965, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 312, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (326.43, 148.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681150 reads in 183.150 CPU sec, 6.039 real sec
[M::mem_process_seqs] Processed 680622 reads in 120.542 CPU sec, 3.951 real sec
sorting block 87, #reads = 1081958 mem = 536878862 peak = 1093033984
[M::process] read 680498 sequences (100000185 bp) in 2.656 CPU sec, 3.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286808, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 311, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (324.92, 147.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680566 sequences (100000268 bp) in 2.376 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286799, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (325.83, 147.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 486, 647)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1413)
[M::mem_pestat] mean and std.dev: (461.27, 303.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1796)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680742 reads in 183.585 CPU sec, 6.090 real sec
[M::mem_process_seqs] Processed 680498 reads in 122.158 CPU sec, 4.053 real sec
sorting block 88, #reads = 1082138 mem = 536880876 peak = 1093033984
[M::process] read 680332 sequences (100000136 bp) in 2.410 CPU sec, 2.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286743, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 312, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (327.03, 149.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 89, #reads = 1082590 mem = 536877712 peak = 1093033984
[M::process] read 680610 sequences (100000273 bp) in 2.548 CPU sec, 2.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286798, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 312, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (328.04, 149.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680566 reads in 181.901 CPU sec, 6.034 real sec
[M::mem_process_seqs] Processed 680332 reads in 120.355 CPU sec, 3.999 real sec
[M::process] read 690564 sequences (100000024 bp) in 2.543 CPU sec, 2.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287206, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 314, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (329.62, 151.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 90, #reads = 1081596 mem = 536881596 peak = 1093033984
[M::process] read 689846 sequences (100000050 bp) in 2.560 CPU sec, 3.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290006, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 263, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (284.54, 136.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680610 reads in 192.679 CPU sec, 6.480 real sec
sorting block 91, #reads = 1082036 mem = 536879505 peak = 1093033984
[M::process] read 690138 sequences (100000182 bp) in 2.086 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289773, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 588, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831)
[M::mem_pestat] mean and std.dev: (565.20, 441.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 267, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (287.29, 136.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 689846 reads in 111.371 CPU sec, 3.638 real sec
[M::process] read 687766 sequences (100000243 bp) in 1.889 CPU sec, 1.901 real sec...
[M::mem_process_seqs] Processed 690564 reads in 308.029 CPU sec, 10.225 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289474, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 265, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (285.20, 134.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 92, #reads = 1081798 mem = 536883977 peak = 1093033984
[M::process] read 687366 sequences (100000029 bp) in 3.261 CPU sec, 3.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288970, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (294.61, 138.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (504, 1177, 1177)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2523)
[M::mem_pestat] mean and std.dev: (845.00, 428.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3196)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690138 reads in 231.023 CPU sec, 7.590 real sec
[M::mem_process_seqs] Processed 687766 reads in 131.837 CPU sec, 4.289 real sec
[M::process] read 686756 sequences (100000030 bp) in 2.453 CPU sec, 2.640 real sec...
sorting block 93, #reads = 1080444 mem = 536879680 peak = 1093033984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288545, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 278, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (296.57, 139.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685646 sequences (100000081 bp) in 2.322 CPU sec, 2.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288742, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (300.29, 141.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687366 reads in 202.937 CPU sec, 6.573 real sec
[M::process] read 685130 sequences (100000206 bp) in 2.121 CPU sec, 2.153 real sec...
[M::mem_process_seqs] Processed 686756 reads in 132.184 CPU sec, 4.199 real sec
sorting block 94, #reads = 1080485 mem = 536881970 peak = 1093033984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288507, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.99, 143.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685116 sequences (100000128 bp) in 2.945 CPU sec, 3.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288703, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (307.89, 144.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685646 reads in 187.796 CPU sec, 6.034 real sec
sorting block 95, #reads = 1081597 mem = 536880558 peak = 1093033984
[M::mem_process_seqs] Processed 685130 reads in 120.573 CPU sec, 3.818 real sec
[M::process] read 684602 sequences (100000148 bp) in 2.146 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287981, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (270, 334, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (142, 462)
[M::mem_pestat] mean and std.dev: (339.25, 38.20)
[M::mem_pestat] low and high boundaries for proper pairs: (78, 526)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (308.70, 144.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 96, #reads = 1083858 mem = 536881881 peak = 1096212480
[M::process] read 684632 sequences (100000193 bp) in 2.652 CPU sec, 2.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287939, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (311.45, 145.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684278 sequences (100000058 bp) in 1.952 CPU sec, 1.952 real sec...
[M::mem_process_seqs] Processed 685116 reads in 196.010 CPU sec, 6.261 real sec
[M::mem_process_seqs] Processed 684602 reads in 132.065 CPU sec, 4.184 real sec
sorting block 97, #reads = 1081612 mem = 536880953 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288309, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (310.82, 144.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684120 sequences (100000221 bp) in 3.078 CPU sec, 3.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287782, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (311.12, 144.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683602 sequences (100000089 bp) in 1.907 CPU sec, 1.907 real sec...
[M::mem_process_seqs] Processed 684632 reads in 183.753 CPU sec, 5.843 real sec
sorting block 98, #reads = 1081638 mem = 536878595 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288127, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 206, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (276.33, 229.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (312.17, 145.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683662 sequences (100000204 bp) in 2.069 CPU sec, 2.070 real sec...
[M::mem_process_seqs] Processed 684120 reads in 102.260 CPU sec, 3.437 real sec
[M::mem_process_seqs] Processed 684278 reads in 248.535 CPU sec, 8.083 real sec
sorting block 99, #reads = 1083247 mem = 536883013 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288335, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 300, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (316.75, 147.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683426 sequences (100000137 bp) in 2.608 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287680, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (315.89, 147.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683602 reads in 198.252 CPU sec, 6.297 real sec
[M::process] read 682688 sequences (100000301 bp) in 1.969 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 683662 reads in 116.257 CPU sec, 3.666 real sec
sorting block 100, #reads = 1082406 mem = 536882460 peak = 1096212480
[M::process] read 681862 sequences (100000297 bp) in 2.530 CPU sec, 2.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286819, 6, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 505, 1262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3382)
[M::mem_pestat] mean and std.dev: (661.53, 508.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4442)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (321.31, 149.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680910 sequences (100000126 bp) in 1.818 CPU sec, 1.827 real sec...
[M::mem_process_seqs] Processed 682688 reads in 96.640 CPU sec, 3.046 real sec
sorting block 101, #reads = 1083450 mem = 536880959 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287491, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (316.32, 147.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286939, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 310, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (326.05, 150.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683426 reads in 261.907 CPU sec, 8.333 real sec
[M::process] read 681442 sequences (100000134 bp) in 2.147 CPU sec, 2.169 real sec...
[M::mem_process_seqs] Processed 681862 reads in 126.886 CPU sec, 4.027 real sec
sorting block 102, #reads = 1082751 mem = 536881296 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286579, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 313, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (328.20, 150.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 389, 728)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712)
[M::mem_pestat] mean and std.dev: (457.67, 232.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2204)
[M::mem_pestat] skip orientation RR
[M::process] read 681714 sequences (100000012 bp) in 2.905 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286872, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (326.11, 150.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680910 reads in 165.037 CPU sec, 5.253 real sec
[M::process] read 681352 sequences (100000031 bp) in 1.958 CPU sec, 1.990 real sec...
[M::mem_process_seqs] Processed 681442 reads in 107.554 CPU sec, 3.394 real sec
sorting block 103, #reads = 1082890 mem = 536878450 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286776, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (326.07, 152.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681094 sequences (100000290 bp) in 2.383 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286971, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (325.75, 151.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681714 reads in 162.540 CPU sec, 5.164 real sec
[M::process] read 682348 sequences (100000046 bp) in 1.856 CPU sec, 1.860 real sec...
sorting block 104, #reads = 1082336 mem = 536884932 peak = 1096212480
[M::mem_process_seqs] Processed 681352 reads in 106.647 CPU sec, 3.363 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286175, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 309, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (327.27, 152.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 105, #reads = 1082652 mem = 536881140 peak = 1096212480
[M::process] read 681952 sequences (100000105 bp) in 2.405 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286545, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 300, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (319.06, 150.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688598 sequences (100000017 bp) in 1.930 CPU sec, 1.933 real sec...
[M::mem_process_seqs] Processed 681094 reads in 166.429 CPU sec, 5.285 real sec
[M::mem_process_seqs] Processed 682348 reads in 110.597 CPU sec, 3.501 real sec
sorting block 106, #reads = 1081327 mem = 536880379 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286368, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (323.18, 152.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688366 sequences (100000145 bp) in 2.402 CPU sec, 2.498 real sec...
[M::process] read 687466 sequences (100000283 bp) in 1.900 CPU sec, 1.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 289108, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 272, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (292.03, 139.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681952 reads in 182.178 CPU sec, 5.789 real sec
[M::mem_process_seqs] Processed 688598 reads in 113.726 CPU sec, 3.604 real sec
sorting block 107, #reads = 1075318 mem = 536878861 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289545, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 273, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (293.30, 139.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686060 sequences (100000220 bp) in 2.508 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289064, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 279, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (298.60, 142.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685570 sequences (100000060 bp) in 1.791 CPU sec, 1.798 real sec...
[M::mem_process_seqs] Processed 688366 reads in 165.661 CPU sec, 5.461 real sec
[M::mem_process_seqs] Processed 687466 reads in 102.861 CPU sec, 3.250 real sec
sorting block 108, #reads = 1085137 mem = 536878501 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288566, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (302.57, 142.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685536 sequences (100000006 bp) in 2.499 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287907, 9, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 131, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (182.88, 129.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (305.85, 144.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686060 reads in 158.268 CPU sec, 5.020 real sec
[M::process] read 685040 sequences (100000123 bp) in 1.866 CPU sec, 1.875 real sec...
sorting block 109, #reads = 1085223 mem = 536877767 peak = 1096212480
[M::mem_process_seqs] Processed 685570 reads in 101.293 CPU sec, 3.195 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288372, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (306.21, 144.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 110, #reads = 1085610 mem = 536879619 peak = 1096212480
[M::process] read 684710 sequences (100000077 bp) in 2.776 CPU sec, 2.896 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288177, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (309.17, 145.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685536 reads in 157.291 CPU sec, 4.984 real sec
[M::process] read 684032 sequences (100000106 bp) in 1.902 CPU sec, 1.912 real sec...
[M::mem_process_seqs] Processed 685040 reads in 102.790 CPU sec, 3.234 real sec
sorting block 111, #reads = 1087068 mem = 536879071 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287782, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (310.51, 145.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683944 sequences (100000195 bp) in 2.440 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287840, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (313.29, 146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684710 reads in 153.967 CPU sec, 4.893 real sec
[M::process] read 683936 sequences (100000031 bp) in 1.963 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 684032 reads in 100.959 CPU sec, 3.175 real sec
sorting block 112, #reads = 1086068 mem = 536877294 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287791, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (313.26, 145.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683862 sequences (100000044 bp) in 2.824 CPU sec, 2.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287977, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 298, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (315.79, 147.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683944 reads in 158.802 CPU sec, 5.042 real sec
[M::process] read 683592 sequences (100000032 bp) in 1.957 CPU sec, 1.979 real sec...
[M::mem_process_seqs] Processed 683936 reads in 103.968 CPU sec, 3.268 real sec
sorting block 113, #reads = 1086302 mem = 536879554 peak = 1096212480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287601, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 298, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (315.18, 147.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683106 sequences (100000073 bp) in 2.548 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287371, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 300, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (317.07, 148.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683862 reads in 162.094 CPU sec, 5.165 real sec
sorting block 114, #reads = 1085293 mem = 536879849 peak = 1096212480
[M::mem_process_seqs] Processed 683592 reads in 106.830 CPU sec, 3.359 real sec
[M::process] read 682734 sequences (100000251 bp) in 2.035 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287456, 12, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 303, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (319.83, 149.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 460, 1070)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3208)
[M::mem_pestat] mean and std.dev: (421.25, 423.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4277)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 115, #reads = 1083118 mem = 536882737 peak = 1105776640
[M::process] read 682582 sequences (100000197 bp) in 2.763 CPU sec, 2.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287572, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 305, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (321.81, 150.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683106 reads in 163.860 CPU sec, 5.203 real sec
[M::process] read 682340 sequences (100000126 bp) in 1.911 CPU sec, 1.920 real sec...
[M::mem_process_seqs] Processed 682734 reads in 108.584 CPU sec, 3.423 real sec
sorting block 116, #reads = 1084020 mem = 536877574 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287201, 3, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 305, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (321.52, 149.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (293, 293, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (313.17, 46.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RR
[M::process] read 682116 sequences (100000043 bp) in 2.518 CPU sec, 2.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287238, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 306, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (321.72, 149.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681908 sequences (100000226 bp) in 1.860 CPU sec, 1.856 real sec...
[M::mem_process_seqs] Processed 682582 reads in 164.783 CPU sec, 5.228 real sec
[M::mem_process_seqs] Processed 682340 reads in 107.971 CPU sec, 3.412 real sec
sorting block 117, #reads = 1082807 mem = 536882812 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286794, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (320.75, 147.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681972 sequences (100000038 bp) in 2.806 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286513, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 811, 1541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4171)
[M::mem_pestat] mean and std.dev: (987.36, 745.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5486)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (319.47, 146.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681986 sequences (100000295 bp) in 1.560 CPU sec, 1.557 real sec...
[M::mem_process_seqs] Processed 682116 reads in 165.952 CPU sec, 5.269 real sec
sorting block 118, #reads = 1083187 mem = 536880851 peak = 1105776640
[M::mem_process_seqs] Processed 681908 reads in 106.522 CPU sec, 3.371 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287121, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (319.92, 146.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 119, #reads = 1082246 mem = 536879949 peak = 1105776640
[M::process] read 682274 sequences (100000220 bp) in 2.515 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286965, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (318.95, 145.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682334 sequences (100000235 bp) in 1.910 CPU sec, 1.906 real sec...
[M::mem_process_seqs] Processed 681972 reads in 165.244 CPU sec, 5.241 real sec
[M::mem_process_seqs] Processed 681986 reads in 108.297 CPU sec, 3.415 real sec
sorting block 120, #reads = 1082008 mem = 536878797 peak = 1105776640
[M::process] read 682370 sequences (100000165 bp) in 2.863 CPU sec, 2.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287446, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (319.42, 148.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 404, 1163)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2931)
[M::mem_pestat] mean and std.dev: (581.00, 390.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3815)
[M::mem_pestat] skip orientation RR
[M::process] read 682222 sequences (100000122 bp) in 1.850 CPU sec, 1.853 real sec...
[M::mem_process_seqs] Processed 682334 reads in 93.356 CPU sec, 2.934 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287353, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (318.30, 146.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287453, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (319.16, 148.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682274 reads in 257.846 CPU sec, 8.174 real sec
[M::process] read 682014 sequences (100000010 bp) in 2.273 CPU sec, 2.287 real sec...
sorting block 121, #reads = 1082274 mem = 536879765 peak = 1105776640
[M::mem_process_seqs] Processed 682370 reads in 127.180 CPU sec, 4.029 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287544, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (319.95, 148.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 122, #reads = 1080819 mem = 536878246 peak = 1105776640
[M::process] read 682336 sequences (100000148 bp) in 2.614 CPU sec, 2.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286637, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (319.74, 148.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 692048 sequences (100000244 bp) in 1.960 CPU sec, 1.962 real sec...
[M::mem_process_seqs] Processed 682222 reads in 169.056 CPU sec, 5.361 real sec
[M::mem_process_seqs] Processed 682014 reads in 113.763 CPU sec, 3.593 real sec
sorting block 123, #reads = 1081326 mem = 536878694 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286519, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 365, 1076)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744)
[M::mem_pestat] mean and std.dev: (450.38, 322.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (320.09, 150.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 690868 sequences (100000215 bp) in 2.751 CPU sec, 2.870 real sec...
[M::process] read 690192 sequences (100000169 bp) in 2.000 CPU sec, 1.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289823, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 256, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (278.18, 133.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682336 reads in 191.814 CPU sec, 6.078 real sec
[M::mem_process_seqs] Processed 692048 reads in 121.782 CPU sec, 3.863 real sec
sorting block 124, #reads = 1073757 mem = 536879952 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 289602, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 262, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (283.79, 136.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (356, 523, 838)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1802)
[M::mem_pestat] mean and std.dev: (555.00, 348.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2284)
[M::mem_pestat] skip orientation RR
[M::process] read 688502 sequences (100000187 bp) in 2.446 CPU sec, 2.545 real sec...
[M::process] read 687840 sequences (100000035 bp) in 1.952 CPU sec, 1.962 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289596, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 265, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (285.72, 136.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690868 reads in 184.058 CPU sec, 5.848 real sec
sorting block 125, #reads = 1080073 mem = 536879149 peak = 1105776640
[M::mem_process_seqs] Processed 690192 reads in 120.652 CPU sec, 3.820 real sec
sorting block 126, #reads = 1079729 mem = 536877702 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289032, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 273, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (292.65, 138.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686348 sequences (100000132 bp) in 2.827 CPU sec, 2.937 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289209, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 277, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (296.56, 140.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685818 sequences (100000244 bp) in 1.952 CPU sec, 1.956 real sec...
[M::mem_process_seqs] Processed 688502 reads in 179.974 CPU sec, 5.702 real sec
[M::mem_process_seqs] Processed 687840 reads in 114.565 CPU sec, 3.623 real sec
sorting block 127, #reads = 1081293 mem = 536879876 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288552, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (301.54, 141.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685126 sequences (100000179 bp) in 2.483 CPU sec, 2.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288015, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (304.50, 142.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (326, 878, 1496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3836)
[M::mem_pestat] mean and std.dev: (1061.36, 922.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5006)
[M::mem_pestat] skip orientation RR
[M::process] read 684622 sequences (100000106 bp) in 1.900 CPU sec, 1.905 real sec...
[M::mem_process_seqs] Processed 686348 reads in 174.116 CPU sec, 5.526 real sec
[M::mem_process_seqs] Processed 685818 reads in 110.841 CPU sec, 3.503 real sec
sorting block 128, #reads = 1080917 mem = 536882133 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288178, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (308.46, 144.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683752 sequences (100000083 bp) in 2.907 CPU sec, 3.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288040, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (309.82, 144.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684108 sequences (100000100 bp) in 1.910 CPU sec, 1.906 real sec...
[M::mem_process_seqs] Processed 685126 reads in 172.402 CPU sec, 5.460 real sec
[M::mem_process_seqs] Processed 684622 reads in 108.662 CPU sec, 3.434 real sec
sorting block 129, #reads = 1082247 mem = 536879928 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287486, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (314.61, 146.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683852 sequences (100000240 bp) in 2.471 CPU sec, 2.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287790, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (315.28, 147.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683130 sequences (100000289 bp) in 1.914 CPU sec, 1.915 real sec...
[M::mem_process_seqs] Processed 683752 reads in 172.146 CPU sec, 5.463 real sec
sorting block 130, #reads = 1081265 mem = 536879367 peak = 1105776640
[M::mem_process_seqs] Processed 684108 reads in 111.424 CPU sec, 3.516 real sec
sorting block 131, #reads = 1079906 mem = 536879910 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287988, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (258, 392, 942)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2310)
[M::mem_pestat] mean and std.dev: (575.64, 458.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2994)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (315.99, 148.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683174 sequences (100000222 bp) in 2.434 CPU sec, 2.540 real sec...
[M::process] read 683228 sequences (100000118 bp) in 1.900 CPU sec, 1.898 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287369, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (316.48, 147.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683852 reads in 171.848 CPU sec, 5.454 real sec
[M::mem_process_seqs] Processed 683130 reads in 112.526 CPU sec, 3.567 real sec
sorting block 132, #reads = 1080844 mem = 536878027 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287213, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (313.57, 145.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683348 sequences (100000167 bp) in 2.471 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287497, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (316.16, 147.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683202 sequences (100000022 bp) in 1.889 CPU sec, 1.887 real sec...
[M::mem_process_seqs] Processed 683174 reads in 169.173 CPU sec, 5.374 real sec
[M::mem_process_seqs] Processed 683228 reads in 111.619 CPU sec, 3.521 real sec
sorting block 133, #reads = 1081209 mem = 536879570 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287256, 10, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 242, 1118)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2938)
[M::mem_pestat] mean and std.dev: (484.15, 368.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3848)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 297, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (315.80, 148.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 22, 60)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174)
[M::mem_pestat] mean and std.dev: (14.88, 18.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 231)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 682844 sequences (100000115 bp) in 2.810 CPU sec, 2.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287826, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (315.21, 147.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682640 sequences (100000192 bp) in 1.880 CPU sec, 1.878 real sec...
[M::mem_process_seqs] Processed 683348 reads in 168.015 CPU sec, 5.327 real sec
[M::mem_process_seqs] Processed 683202 reads in 109.356 CPU sec, 3.453 real sec
sorting block 134, #reads = 1082925 mem = 536879069 peak = 1105776640
[M::process] read 682552 sequences (100000188 bp) in 2.594 CPU sec, 2.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287180, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (317.42, 148.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682394 sequences (100000077 bp) in 1.837 CPU sec, 1.842 real sec...
[M::mem_process_seqs] Processed 682640 reads in 93.659 CPU sec, 2.946 real sec
sorting block 135, #reads = 1081956 mem = 536877908 peak = 1105776640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287544, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (317.77, 149.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287169, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 300, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (317.57, 147.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682258 sequences (100000032 bp) in 1.991 CPU sec, 1.995 real sec...
[M::mem_process_seqs] Processed 682844 reads in 251.392 CPU sec, 7.968 real sec
[M::mem_process_seqs] Processed 682552 reads in 122.068 CPU sec, 3.878 real sec
sorting block 136, #reads = 1082893 mem = 536876936 peak = 1115766784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287074, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (318.44, 148.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681788 sequences (100000007 bp) in 3.059 CPU sec, 3.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287160, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (319.76, 149.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682394 reads in 163.450 CPU sec, 5.175 real sec
[M::process] read 681718 sequences (100000157 bp) in 1.879 CPU sec, 1.891 real sec...
sorting block 137, #reads = 1082672 mem = 536877780 peak = 1115766784
[M::mem_process_seqs] Processed 682258 reads in 107.222 CPU sec, 3.382 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287314, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (322.25, 150.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 138, #reads = 1084107 mem = 536879863 peak = 1115766784
[M::process] read 681896 sequences (100000083 bp) in 2.752 CPU sec, 2.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287569, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (323.21, 150.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 372696 sequences (54583502 bp) in 1.260 CPU sec, 1.260 real sec...
[M::mem_process_seqs] Processed 681788 reads in 156.712 CPU sec, 4.979 real sec
[M::mem_process_seqs] Processed 681718 reads in 101.620 CPU sec, 3.217 real sec
sorting block 139, #reads = 1083234 mem = 536877446 peak = 1115766784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287105, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (323.70, 152.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 156647, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (320.25, 151.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681896 reads in 126.688 CPU sec, 4.112 real sec
[M::mem_process_seqs] Processed 372696 reads in 67.349 CPU sec, 2.178 real sec
sorting block 140, #reads = 1084180 mem = 536879184 peak = 1115766784
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 152656902
overall: 70006059008 mem 21708.721 user 524.331 sys 733.969 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cAd-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:659_cAd-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cAd-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cAd-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cAd-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 734.013 sec; CPU: 22233.057 sec
sorting block 141, #reads = 335787 mem = 167040285 peak = 1115766784
uniq header count 1
merging 142 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 7995222893
output reads: 153245792
bam_mem_sort: 142 calls 93.823 user 1.910 sys 97.805 real
bam_write: 142 calls 63.540 user 4.871 sys 107.308 real
execute: 1 calls 47.439 user 1.556 sys 788.156 real
merge_files: 1 calls 47.420 user 1.530 sys 53.823 real
parse_chunk: 2261 calls 101.851 user 27.273 sys 147.926 real
read_chunk: 6095 calls 54.741 user 40.840 sys 688.769 real
sort_block: 1 calls 33.826 user 40.035 sys 661.258 real
write_chunk: 3832 calls 47.018 user 1.234 sys 52.816 real
overall: 3289485312 mem 2128.027 user 96.852 sys 788.174 real
ls: cannot access '*.cram': No such file or directory