cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
Received only 1 licenses out of 31 requested for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
Received only 1 licenses out of 31 requested for sentieon:klib
Received only 1 licenses out of 31 requested for sentieon:klib
Received only 1 licenses out of 31 requested for sentieon:klib
Received only 30 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 1629.017 CPU sec, 290.313 real sec
[M::process] read 702842 sequences (100000042 bp) in 0.979 CPU sec, 0.981 real sec...
[M::process] read 701908 sequences (100000110 bp) in 1.130 CPU sec, 1.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292129, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 177, 177)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 265)
[M::mem_pestat] mean and std.dev: (160.30, 31.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 309)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.11, 113.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701530 sequences (100000159 bp) in 1.169 CPU sec, 1.169 real sec...
[M::process] read 701054 sequences (100000196 bp) in 1.180 CPU sec, 1.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291905, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.08, 115.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702842 reads in 128.668 CPU sec, 4.179 real sec
[M::mem_process_seqs] Processed 701908 reads in 79.502 CPU sec, 2.608 real sec
sorting block 0, #reads = 1096167 mem = 536878616 peak = 597893120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292056, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 224, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168)
[M::mem_pestat] mean and std.dev: (218.70, 114.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1503)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.12, 116.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699776 sequences (100000079 bp) in 1.823 CPU sec, 1.990 real sec...
[M::process] read 699310 sequences (100000049 bp) in 1.240 CPU sec, 1.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292169, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.12, 117.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
Received only 30 licenses out of 31 requested for sentieon:klib
[M::mem_process_seqs] Processed 701530 reads in 128.725 CPU sec, 4.234 real sec
[M::mem_process_seqs] Processed 701054 reads in 80.420 CPU sec, 2.652 real sec
sorting block 1, #reads = 1094995 mem = 536882165 peak = 723722240
[M::process] read 698882 sequences (100000203 bp) in 1.635 CPU sec, 1.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291555, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.73, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698414 sequences (100000051 bp) in 1.258 CPU sec, 1.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291535, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.71, 120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 330, 1583)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4353)
[M::mem_pestat] mean and std.dev: (727.50, 641.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5738)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 290804, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.58, 121.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699776 reads in 180.940 CPU sec, 5.989 real sec
[M::mem_process_seqs] Processed 699310 reads in 134.069 CPU sec, 4.465 real sec
sorting block 2, #reads = 1094411 mem = 536876767 peak = 840781824
[M::mem_process_seqs] Processed 698882 reads in 145.753 CPU sec, 4.875 real sec
sorting block 3, #reads = 1094014 mem = 536881154 peak = 840781824
[M::process] read 698200 sequences (100000070 bp) in 2.001 CPU sec, 2.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291121, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.63, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697964 sequences (100000013 bp) in 1.681 CPU sec, 1.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290611, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.82, 119.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698414 reads in 139.826 CPU sec, 4.619 real sec
[M::mem_process_seqs] Processed 698200 reads in 90.016 CPU sec, 2.945 real sec
sorting block 4, #reads = 1093684 mem = 536880441 peak = 840781824
[M::process] read 698642 sequences (100000202 bp) in 1.826 CPU sec, 1.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290998, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.57, 119.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697986 sequences (100000292 bp) in 1.856 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290701, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.72, 119.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 212, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 399)
[M::mem_pestat] mean and std.dev: (207.50, 81.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RR
[M::process] read 697704 sequences (100000217 bp) in 1.293 CPU sec, 1.304 real sec...
[M::mem_process_seqs] Processed 697964 reads in 134.148 CPU sec, 4.404 real sec
sorting block 5, #reads = 1093003 mem = 536878882 peak = 840781824
[M::mem_process_seqs] Processed 698642 reads in 84.097 CPU sec, 2.756 real sec
sorting block 6, #reads = 1093166 mem = 536878813 peak = 840781824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290361, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 575, 898)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2344)
[M::mem_pestat] mean and std.dev: (545.92, 425.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3067)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (249.08, 118.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697074 sequences (100000232 bp) in 2.062 CPU sec, 2.191 real sec...
[M::process] read 696692 sequences (100000047 bp) in 1.320 CPU sec, 1.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290842, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.17, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697986 reads in 135.312 CPU sec, 4.460 real sec
[M::mem_process_seqs] Processed 697704 reads in 83.398 CPU sec, 2.756 real sec
sorting block 7, #reads = 1093243 mem = 536878248 peak = 840781824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289885, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 329, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (277.89, 94.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.07, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695886 sequences (100000148 bp) in 2.040 CPU sec, 2.169 real sec...
[M::process] read 696210 sequences (100000220 bp) in 1.330 CPU sec, 1.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290351, 8, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (587, 589, 1291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2699)
[M::mem_pestat] mean and std.dev: (796.40, 588.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3403)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.85, 120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697074 reads in 134.193 CPU sec, 4.419 real sec
[M::mem_process_seqs] Processed 696692 reads in 83.082 CPU sec, 2.728 real sec
sorting block 8, #reads = 1092424 mem = 536878728 peak = 840781824
[M::process] read 696072 sequences (100000279 bp) in 2.130 CPU sec, 2.273 real sec...
[M::process] read 703582 sequences (100000038 bp) in 1.340 CPU sec, 1.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290135, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.32, 122.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696210 reads in 74.649 CPU sec, 2.449 real sec
sorting block 9, #reads = 1092749 mem = 536879725 peak = 840781824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289596, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.28, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290013, 11, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.86, 123.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (374, 1976, 2548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6896)
[M::mem_pestat] mean and std.dev: (1683.18, 1207.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9070)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 702540 sequences (100000025 bp) in 1.543 CPU sec, 1.552 real sec...
[M::mem_process_seqs] Processed 695886 reads in 205.115 CPU sec, 6.761 real sec
[M::mem_process_seqs] Processed 696072 reads in 95.389 CPU sec, 3.165 real sec
sorting block 10, #reads = 1091325 mem = 536877352 peak = 840781824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293067, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (239.85, 115.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702218 sequences (100000230 bp) in 2.264 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292652, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.72, 117.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701608 sequences (100000036 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_process_seqs] Processed 703582 reads in 134.232 CPU sec, 4.425 real sec
[M::mem_process_seqs] Processed 702540 reads in 83.360 CPU sec, 2.736 real sec
sorting block 11, #reads = 1094579 mem = 536877899 peak = 840781824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292007, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (243.98, 118.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700690 sequences (100000007 bp) in 1.923 CPU sec, 2.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 292255, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 166, 1086)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2960)
[M::mem_pestat] mean and std.dev: (608.80, 583.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3897)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.47, 119.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 597, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1496)
[M::mem_pestat] mean and std.dev: (382.33, 206.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1937)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 699948 sequences (100000000 bp) in 1.400 CPU sec, 1.404 real sec...
[M::mem_process_seqs] Processed 702218 reads in 137.175 CPU sec, 4.532 real sec
sorting block 12, #reads = 1098259 mem = 536877542 peak = 840781824
[M::mem_process_seqs] Processed 701608 reads in 84.394 CPU sec, 2.768 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291638, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (248.04, 120.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1097970 mem = 536877283 peak = 896626688
[M::process] read 699820 sequences (100000134 bp) in 2.194 CPU sec, 2.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291259, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.75, 121.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1459, 3150, 3150)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6532)
[M::mem_pestat] mean and std.dev: (2168.50, 1187.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8223)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 699346 sequences (100000176 bp) in 1.400 CPU sec, 1.403 real sec...
[M::mem_process_seqs] Processed 700690 reads in 128.952 CPU sec, 4.232 real sec
[M::mem_process_seqs] Processed 699948 reads in 80.998 CPU sec, 2.647 real sec
sorting block 14, #reads = 1096162 mem = 536883173 peak = 896626688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291213, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (250.79, 122.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699316 sequences (100000004 bp) in 1.907 CPU sec, 2.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291358, 9, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 195, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 349)
[M::mem_pestat] mean and std.dev: (177.89, 41.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 413)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.25, 122.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698698 sequences (100000279 bp) in 1.410 CPU sec, 1.413 real sec...
[M::mem_process_seqs] Processed 699820 reads in 129.284 CPU sec, 4.260 real sec
[M::mem_process_seqs] Processed 699346 reads in 81.850 CPU sec, 2.682 real sec
sorting block 15, #reads = 1095116 mem = 536878565 peak = 896626688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291079, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.83, 121.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 268, 1390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3750)
[M::mem_pestat] mean and std.dev: (634.10, 663.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4930)
[M::mem_pestat] skip orientation RR
[M::process] read 698716 sequences (100000063 bp) in 2.282 CPU sec, 2.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291031, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.93, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698760 sequences (100000171 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_process_seqs] Processed 699316 reads in 129.401 CPU sec, 4.245 real sec
[M::mem_process_seqs] Processed 698698 reads in 81.548 CPU sec, 2.661 real sec
sorting block 16, #reads = 1095108 mem = 536879753 peak = 896626688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291147, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (375, 519, 2771)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7563)
[M::mem_pestat] mean and std.dev: (1554.70, 2074.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9959)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (247.77, 118.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
Received only 30 licenses out of 31 requested for sentieon:klib
[M::process] read 698076 sequences (100000086 bp) in 1.932 CPU sec, 2.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291344, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 267, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (282.55, 223.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (248.19, 118.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697562 sequences (100000079 bp) in 1.390 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 698716 reads in 129.331 CPU sec, 4.255 real sec
sorting block 17, #reads = 1094511 mem = 536881000 peak = 896626688
[M::mem_process_seqs] Processed 698760 reads in 81.406 CPU sec, 2.661 real sec
sorting block 18, #reads = 1093004 mem = 536882376 peak = 896626688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290482, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.46, 118.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 300, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (312.56, 107.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RR
[M::process] read 697368 sequences (100000069 bp) in 2.019 CPU sec, 2.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291062, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 180, 1049)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2809)
[M::mem_pestat] mean and std.dev: (362.50, 319.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3689)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.48, 118.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697126 sequences (100000253 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_process_seqs] Processed 698076 reads in 129.716 CPU sec, 4.266 real sec
[M::mem_process_seqs] Processed 697562 reads in 81.710 CPU sec, 2.669 real sec
sorting block 19, #reads = 1093588 mem = 536884680 peak = 896626688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290370, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.02, 120.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697114 sequences (100000058 bp) in 2.026 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290277, 10, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.00, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (357, 566, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1635)
[M::mem_pestat] mean and std.dev: (703.30, 466.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2569)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 176, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546)
[M::mem_pestat] mean and std.dev: (207.73, 73.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698742 sequences (100000065 bp) in 1.400 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 697368 reads in 129.902 CPU sec, 4.272 real sec
[M::mem_process_seqs] Processed 697126 reads in 81.903 CPU sec, 2.677 real sec
sorting block 20, #reads = 1092957 mem = 536878037 peak = 896626688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290300, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.86, 121.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704138 sequences (100000089 bp) in 2.311 CPU sec, 2.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291192, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.73, 121.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704110 sequences (100000005 bp) in 1.406 CPU sec, 1.416 real sec...
[M::mem_process_seqs] Processed 697114 reads in 131.554 CPU sec, 4.317 real sec
[M::mem_process_seqs] Processed 698742 reads in 83.763 CPU sec, 2.733 real sec
sorting block 21, #reads = 1092072 mem = 536882214 peak = 896626688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292404, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 243, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (204.18, 106.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (239.26, 115.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 22, #reads = 1092599 mem = 536879500 peak = 968273920
[M::process] read 702506 sequences (100000151 bp) in 1.980 CPU sec, 2.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293015, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (239.62, 115.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701492 sequences (100000179 bp) in 1.390 CPU sec, 1.387 real sec...
[M::mem_process_seqs] Processed 704138 reads in 131.158 CPU sec, 4.314 real sec
[M::mem_process_seqs] Processed 704110 reads in 83.143 CPU sec, 2.718 real sec
sorting block 23, #reads = 1097197 mem = 536880723 peak = 968273920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292266, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.69, 116.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700340 sequences (100000162 bp) in 2.065 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291695, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.74, 118.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699864 sequences (100000130 bp) in 1.380 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 702506 reads in 131.120 CPU sec, 4.311 real sec
[M::mem_process_seqs] Processed 701492 reads in 82.399 CPU sec, 2.696 real sec
sorting block 24, #reads = 1096757 mem = 536876890 peak = 968273920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291696, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.87, 119.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699256 sequences (100000200 bp) in 2.028 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291495, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.88, 119.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699064 sequences (100000138 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_process_seqs] Processed 700340 reads in 129.214 CPU sec, 4.260 real sec
[M::mem_process_seqs] Processed 699864 reads in 80.896 CPU sec, 2.649 real sec
sorting block 25, #reads = 1095997 mem = 536879502 peak = 968273920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291214, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.84, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698758 sequences (100000170 bp) in 2.008 CPU sec, 2.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291305, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.15, 121.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 319, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (89, 574)
[M::mem_pestat] mean and std.dev: (283.44, 70.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 671)
[M::mem_pestat] skip orientation RR
[M::process] read 698586 sequences (100000115 bp) in 1.370 CPU sec, 1.371 real sec...
[M::mem_process_seqs] Processed 699256 reads in 129.606 CPU sec, 4.262 real sec
sorting block 26, #reads = 1095168 mem = 536878474 peak = 968273920
[M::mem_process_seqs] Processed 699064 reads in 81.589 CPU sec, 2.665 real sec
sorting block 27, #reads = 1095075 mem = 536880365 peak = 1012035584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291238, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.29, 121.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 330, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (278.42, 145.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RR
[M::process] read 698110 sequences (100000294 bp) in 2.000 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290404, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.85, 118.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698388 sequences (100000096 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 698758 reads in 130.292 CPU sec, 4.284 real sec
[M::mem_process_seqs] Processed 698586 reads in 82.232 CPU sec, 2.687 real sec
sorting block 28, #reads = 1094075 mem = 536877950 peak = 1012035584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290898, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 196, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (223.80, 128.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.54, 118.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697812 sequences (100000014 bp) in 2.064 CPU sec, 2.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291011, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (291, 1014, 1503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3927)
[M::mem_pestat] mean and std.dev: (618.12, 456.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5139)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.27, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697604 sequences (100000293 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 698110 reads in 130.593 CPU sec, 4.290 real sec
[M::mem_process_seqs] Processed 698388 reads in 82.056 CPU sec, 2.685 real sec
sorting block 29, #reads = 1092538 mem = 536879079 peak = 1012035584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290835, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.41, 119.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697682 sequences (100000167 bp) in 2.034 CPU sec, 2.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290900, 10, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.36, 118.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (602, 764, 1097)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2087)
[M::mem_pestat] mean and std.dev: (863.70, 293.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2582)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 697482 sequences (100000013 bp) in 1.380 CPU sec, 1.387 real sec...
[M::mem_process_seqs] Processed 697812 reads in 131.857 CPU sec, 4.333 real sec
[M::mem_process_seqs] Processed 697604 reads in 83.302 CPU sec, 2.724 real sec
sorting block 30, #reads = 1093099 mem = 536877550 peak = 1012035584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290892, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.97, 120.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1092051 mem = 536878555 peak = 1023254528
[M::process] read 697322 sequences (100000154 bp) in 2.106 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290520, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.68, 120.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701432 sequences (100000207 bp) in 1.450 CPU sec, 1.446 real sec...
[M::mem_process_seqs] Processed 697682 reads in 133.211 CPU sec, 4.375 real sec
[M::mem_process_seqs] Processed 697482 reads in 83.390 CPU sec, 2.723 real sec
sorting block 32, #reads = 1090802 mem = 536878420 peak = 1023254528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290381, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.41, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703014 sequences (100000175 bp) in 2.057 CPU sec, 2.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292129, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.79, 116.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 341, 2907)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8205)
[M::mem_pestat] mean and std.dev: (1471.60, 1800.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10854)
[M::mem_pestat] skip orientation RR
[M::process] read 702692 sequences (100000014 bp) in 1.380 CPU sec, 1.386 real sec...
[M::mem_process_seqs] Processed 697322 reads in 133.367 CPU sec, 4.389 real sec
[M::mem_process_seqs] Processed 701432 reads in 84.460 CPU sec, 2.759 real sec
sorting block 33, #reads = 1091525 mem = 536881107 peak = 1023254528
[M::process] read 701784 sequences (100000126 bp) in 2.050 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292189, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.64, 117.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702692 reads in 71.072 CPU sec, 2.235 real sec
[M::process] read 701326 sequences (100000289 bp) in 1.470 CPU sec, 1.484 real sec...
sorting block 34, #reads = 1095064 mem = 536878112 peak = 1023254528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292519, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (242.04, 116.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 292119, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (244.61, 118.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703014 reads in 196.946 CPU sec, 6.305 real sec
[M::mem_process_seqs] Processed 701784 reads in 93.021 CPU sec, 2.947 real sec
[M::process] read 700368 sequences (100000024 bp) in 1.886 CPU sec, 1.920 real sec...
sorting block 35, #reads = 1096892 mem = 536879232 peak = 1023254528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291527, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.19, 119.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 267, 792)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870)
[M::mem_pestat] mean and std.dev: (418.85, 288.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2409)
[M::mem_pestat] skip orientation RR
[M::process] read 699936 sequences (100000039 bp) in 2.062 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291341, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (248.78, 120.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701326 reads in 128.591 CPU sec, 4.103 real sec
[M::mem_process_seqs] Processed 700368 reads in 82.128 CPU sec, 2.592 real sec
[M::process] read 699538 sequences (100000188 bp) in 1.549 CPU sec, 1.596 real sec...
sorting block 36, #reads = 1096544 mem = 536879134 peak = 1023254528
[M::process] read 699194 sequences (100000170 bp) in 2.231 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291156, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 254, 953)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2383)
[M::mem_pestat] mean and std.dev: (396.50, 349.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3098)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.65, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699538 reads in 71.336 CPU sec, 2.243 real sec
sorting block 37, #reads = 1094275 mem = 536879870 peak = 1023254528
[M::process] read 700312 sequences (100000256 bp) in 1.501 CPU sec, 1.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291506, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.47, 120.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 291463, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 325, 943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2499)
[M::mem_pestat] mean and std.dev: (437.33, 339.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3277)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.76, 121.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699936 reads in 199.147 CPU sec, 6.315 real sec
[M::process] read 699146 sequences (100000184 bp) in 1.836 CPU sec, 1.868 real sec...
[M::mem_process_seqs] Processed 699194 reads in 94.193 CPU sec, 2.984 real sec
sorting block 38, #reads = 1094292 mem = 536879997 peak = 1031880704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291540, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.16, 117.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 441, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (295.80, 152.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1384)
[M::mem_pestat] skip orientation RR
[M::process] read 698606 sequences (100000020 bp) in 2.204 CPU sec, 2.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291369, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (246.86, 117.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 171, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (178.67, 43.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 522)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700312 reads in 130.180 CPU sec, 4.152 real sec
[M::mem_process_seqs] Processed 699146 reads in 83.102 CPU sec, 2.620 real sec
sorting block 39, #reads = 1094881 mem = 536879197 peak = 1031880704
[M::process] read 698296 sequences (100000089 bp) in 1.666 CPU sec, 1.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290504, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (304, 321, 1206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3010)
[M::mem_pestat] mean and std.dev: (852.79, 938.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4606)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.76, 117.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 40, #reads = 1094476 mem = 536883424 peak = 1031880704
[M::process] read 697996 sequences (100000137 bp) in 1.968 CPU sec, 2.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290775, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (247.49, 116.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698606 reads in 130.757 CPU sec, 4.166 real sec
[M::process] read 697892 sequences (100000176 bp) in 1.501 CPU sec, 1.521 real sec...
[M::mem_process_seqs] Processed 698296 reads in 82.849 CPU sec, 2.610 real sec
sorting block 41, #reads = 1092522 mem = 536880683 peak = 1031880704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290632, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (248.56, 117.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697650 sequences (100000164 bp) in 1.962 CPU sec, 2.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290162, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 225, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (247.71, 116.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697996 reads in 130.217 CPU sec, 4.147 real sec
[M::process] read 697542 sequences (100000180 bp) in 1.390 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 697892 reads in 82.968 CPU sec, 2.621 real sec
sorting block 42, #reads = 1092493 mem = 536877765 peak = 1031880704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290812, 9, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 280, 1737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4771)
[M::mem_pestat] mean and std.dev: (816.70, 790.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6288)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.75, 119.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696924 sequences (100000033 bp) in 2.258 CPU sec, 2.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290444, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 303, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1484)
[M::mem_pestat] mean and std.dev: (343.33, 196.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1921)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.02, 119.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697650 reads in 130.712 CPU sec, 4.150 real sec
[M::process] read 708332 sequences (100000068 bp) in 1.450 CPU sec, 1.450 real sec...
[M::mem_process_seqs] Processed 697542 reads in 82.617 CPU sec, 2.609 real sec
sorting block 43, #reads = 1091634 mem = 536880607 peak = 1031880704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289939, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 203, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 433)
[M::mem_pestat] mean and std.dev: (182.12, 46.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 518)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.12, 121.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 705960 sequences (100000011 bp) in 1.954 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 294041, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (223.57, 104.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704158 sequences (100000224 bp) in 1.420 CPU sec, 1.415 real sec...
[M::mem_process_seqs] Processed 696924 reads in 135.850 CPU sec, 4.324 real sec
sorting block 44, #reads = 1091368 mem = 536889280 peak = 1031880704
[M::mem_process_seqs] Processed 708332 reads in 87.648 CPU sec, 2.774 real sec
sorting block 45, #reads = 1093705 mem = 536878918 peak = 1031880704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293826, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (105, 260)
[M::mem_pestat] mean and std.dev: (172.50, 23.76)
[M::mem_pestat] low and high boundaries for proper pairs: (74, 291)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.63, 110.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702922 sequences (100000296 bp) in 2.039 CPU sec, 2.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 292863, 12, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 177, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 315)
[M::mem_pestat] mean and std.dev: (158.73, 40.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 371)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (236.71, 114.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (333, 542, 2999)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8331)
[M::mem_pestat] mean and std.dev: (1093.25, 1249.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10997)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 219, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124)
[M::mem_pestat] mean and std.dev: (227.44, 144.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1453)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 701886 sequences (100000085 bp) in 1.430 CPU sec, 1.429 real sec...
[M::mem_process_seqs] Processed 705960 reads in 133.983 CPU sec, 4.260 real sec
[M::mem_process_seqs] Processed 704158 reads in 83.282 CPU sec, 2.633 real sec
sorting block 46, #reads = 1096480 mem = 536879815 peak = 1031880704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292905, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.77, 116.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701400 sequences (100000226 bp) in 1.972 CPU sec, 2.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292695, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.29, 117.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700922 sequences (100000034 bp) in 1.420 CPU sec, 1.424 real sec...
[M::mem_process_seqs] Processed 702922 reads in 132.769 CPU sec, 4.226 real sec
[M::mem_process_seqs] Processed 701886 reads in 83.377 CPU sec, 2.639 real sec
sorting block 47, #reads = 1096179 mem = 536877050 peak = 1031880704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291822, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.04, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700358 sequences (100000242 bp) in 2.255 CPU sec, 2.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292162, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.75, 120.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701400 reads in 134.063 CPU sec, 4.264 real sec
[M::process] read 699562 sequences (100000085 bp) in 1.469 CPU sec, 1.480 real sec...
sorting block 48, #reads = 1095197 mem = 536877484 peak = 1031880704
[M::mem_process_seqs] Processed 700922 reads in 84.994 CPU sec, 2.683 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291064, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.30, 119.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1094265 mem = 536879152 peak = 1050828800
[M::process] read 699274 sequences (100000199 bp) in 2.033 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291556, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.80, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699062 sequences (100000205 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_process_seqs] Processed 700358 reads in 136.535 CPU sec, 4.345 real sec
[M::mem_process_seqs] Processed 699562 reads in 86.581 CPU sec, 2.742 real sec
sorting block 50, #reads = 1093362 mem = 536886415 peak = 1050828800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291267, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.00, 119.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 198, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (280.45, 183.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RR
[M::process] read 698648 sequences (100000212 bp) in 2.162 CPU sec, 2.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291108, 7, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 373, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114)
[M::mem_pestat] mean and std.dev: (319.93, 154.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1414)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.50, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 185, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (99, 314)
[M::mem_pestat] mean and std.dev: (183.38, 25.99)
[M::mem_pestat] low and high boundaries for proper pairs: (56, 357)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698378 sequences (100000088 bp) in 1.430 CPU sec, 1.436 real sec...
[M::mem_process_seqs] Processed 699274 reads in 137.039 CPU sec, 4.358 real sec
[M::mem_process_seqs] Processed 699062 reads in 87.186 CPU sec, 2.754 real sec
sorting block 51, #reads = 1092342 mem = 536879512 peak = 1050828800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290718, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.54, 119.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698232 sequences (100000202 bp) in 2.045 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290436, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.67, 119.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698042 sequences (100000060 bp) in 1.420 CPU sec, 1.414 real sec...
[M::mem_process_seqs] Processed 698648 reads in 137.953 CPU sec, 4.391 real sec
[M::mem_process_seqs] Processed 698378 reads in 87.305 CPU sec, 2.763 real sec
sorting block 52, #reads = 1090952 mem = 536876364 peak = 1050828800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291020, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.83, 118.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697972 sequences (100000006 bp) in 2.302 CPU sec, 2.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290878, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 246, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (243.64, 73.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.63, 119.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698232 reads in 137.492 CPU sec, 4.369 real sec
[M::process] read 698188 sequences (100000184 bp) in 1.560 CPU sec, 1.555 real sec...
sorting block 53, #reads = 1089388 mem = 536877506 peak = 1050828800
[M::mem_process_seqs] Processed 698042 reads in 86.800 CPU sec, 2.742 real sec
sorting block 54, #reads = 1090870 mem = 536880006 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290985, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.07, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 710336 sequences (100000028 bp) in 2.285 CPU sec, 2.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290492, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.91, 120.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697972 reads in 139.035 CPU sec, 4.423 real sec
[M::process] read 709478 sequences (100000139 bp) in 1.788 CPU sec, 1.817 real sec...
[M::mem_process_seqs] Processed 698188 reads in 89.555 CPU sec, 2.821 real sec
sorting block 55, #reads = 1091093 mem = 536877947 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 294979, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 197, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (219.05, 102.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706966 sequences (100000067 bp) in 2.726 CPU sec, 2.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 294596, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 199, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (221.98, 104.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 377, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217)
[M::mem_pestat] mean and std.dev: (315.67, 172.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1581)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 710336 reads in 146.758 CPU sec, 4.666 real sec
[M::mem_process_seqs] Processed 709478 reads in 94.713 CPU sec, 2.985 real sec
[M::process] read 705916 sequences (100000233 bp) in 1.949 CPU sec, 1.981 real sec...
sorting block 56, #reads = 1092591 mem = 536877877 peak = 1152053248
[M::process] read 705168 sequences (100000104 bp) in 2.420 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293722, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (231.64, 110.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705916 reads in 79.238 CPU sec, 2.492 real sec
sorting block 57, #reads = 1093582 mem = 536876335 peak = 1152053248
[M::process] read 704442 sequences (100000165 bp) in 2.017 CPU sec, 2.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293899, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.48, 108.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293730, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.34, 113.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706966 reads in 222.227 CPU sec, 7.056 real sec
[M::mem_process_seqs] Processed 705168 reads in 104.084 CPU sec, 3.292 real sec
[M::process] read 703822 sequences (100000054 bp) in 2.064 CPU sec, 2.117 real sec...
sorting block 58, #reads = 1093852 mem = 536889031 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293073, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (236.98, 114.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702690 sequences (100000071 bp) in 2.390 CPU sec, 2.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293011, 0, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 190, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (180.75, 44.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.68, 115.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 402, 1149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3079)
[M::mem_pestat] mean and std.dev: (418.75, 352.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4044)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 704442 reads in 140.485 CPU sec, 4.472 real sec
[M::mem_process_seqs] Processed 703822 reads in 88.614 CPU sec, 2.795 real sec
sorting block 59, #reads = 1093818 mem = 536879517 peak = 1152053248
[M::process] read 701060 sequences (100000187 bp) in 1.806 CPU sec, 1.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292404, 10, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 136, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1687)
[M::mem_pestat] mean and std.dev: (295.44, 245.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2209)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (242.65, 118.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 282, 628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1590)
[M::mem_pestat] mean and std.dev: (358.90, 286.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2071)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
sorting block 60, #reads = 1092920 mem = 536880046 peak = 1152053248
[M::process] read 700172 sequences (100000053 bp) in 2.443 CPU sec, 2.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292132, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.59, 119.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 220, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (298.50, 216.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702690 reads in 140.922 CPU sec, 4.486 real sec
[M::process] read 699554 sequences (100000014 bp) in 1.927 CPU sec, 1.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291794, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.81, 119.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700172 reads in 76.779 CPU sec, 2.423 real sec
sorting block 61, #reads = 1092540 mem = 536880963 peak = 1152053248
[M::process] read 699044 sequences (100000286 bp) in 2.471 CPU sec, 2.524 real sec...
[M::mem_process_seqs] Processed 701060 reads in 203.374 CPU sec, 6.425 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291109, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.90, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 62, #reads = 1091702 mem = 536883307 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291750, 9, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.80, 118.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 207, 943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2553)
[M::mem_pestat] mean and std.dev: (294.70, 270.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3358)
[M::mem_pestat] skip orientation RR
[M::process] read 699200 sequences (100000002 bp) in 2.876 CPU sec, 3.017 real sec...
[M::mem_process_seqs] Processed 699554 reads in 151.041 CPU sec, 4.798 real sec
[M::mem_process_seqs] Processed 699044 reads in 87.655 CPU sec, 2.782 real sec
[M::process] read 698512 sequences (100000280 bp) in 2.468 CPU sec, 2.616 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291345, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.24, 118.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1090714 mem = 536879349 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291288, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.31, 119.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698058 sequences (100000234 bp) in 2.083 CPU sec, 2.160 real sec...
[M::mem_process_seqs] Processed 699200 reads in 138.727 CPU sec, 4.436 real sec
[M::mem_process_seqs] Processed 698512 reads in 86.945 CPU sec, 2.755 real sec
sorting block 64, #reads = 1091709 mem = 536878507 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290776, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.62, 120.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (343, 409, 1170)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2824)
[M::mem_pestat] mean and std.dev: (687.10, 547.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3651)
[M::mem_pestat] skip orientation RR
[M::process] read 698578 sequences (100000236 bp) in 2.553 CPU sec, 2.711 real sec...
[M::mem_process_seqs] Processed 698058 reads in 76.175 CPU sec, 2.515 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290843, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 258, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (271.60, 108.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.25, 119.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 65, #reads = 1090721 mem = 536879366 peak = 1152053248
[M::mem_process_seqs] Processed 698578 reads in 78.301 CPU sec, 2.554 real sec
[M::process] read 707204 sequences (100000288 bp) in 2.937 CPU sec, 3.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293398, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.13, 108.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 707376 sequences (100000096 bp) in 2.400 CPU sec, 2.539 real sec...
[M::mem_process_seqs] Processed 707204 reads in 80.401 CPU sec, 2.618 real sec
sorting block 66, #reads = 1088545 mem = 536879613 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 294476, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.05, 109.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707376 reads in 74.695 CPU sec, 2.415 real sec
[M::process] read 706320 sequences (100000285 bp) in 2.452 CPU sec, 2.589 real sec...
sorting block 67, #reads = 1095448 mem = 536881011 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 293935, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 285, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (197.60, 89.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (232.03, 111.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 706320 reads in 73.722 CPU sec, 2.389 real sec
[M::process] read 704772 sequences (100000230 bp) in 2.336 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 292733, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 269, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (259.43, 137.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (235.67, 112.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703264 sequences (100000026 bp) in 2.063 CPU sec, 2.178 real sec...
[M::mem_process_seqs] Processed 704772 reads in 73.231 CPU sec, 2.380 real sec
sorting block 68, #reads = 1098898 mem = 536878740 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292522, 4, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 323, 1259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3227)
[M::mem_pestat] mean and std.dev: (645.27, 661.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4211)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.83, 114.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702808 sequences (100000139 bp) in 2.191 CPU sec, 2.349 real sec...
[M::mem_process_seqs] Processed 703264 reads in 73.844 CPU sec, 2.384 real sec
sorting block 69, #reads = 1096702 mem = 536877644 peak = 1152053248
[M::process] read 703042 sequences (100000126 bp) in 2.086 CPU sec, 2.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292315, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.73, 115.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702278 sequences (100000141 bp) in 1.360 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292902, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.48, 116.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702808 reads in 139.030 CPU sec, 4.373 real sec
[M::mem_process_seqs] Processed 703042 reads in 85.205 CPU sec, 2.675 real sec
[M::process] read 701596 sequences (100000074 bp) in 1.524 CPU sec, 1.565 real sec...
sorting block 70, #reads = 1093730 mem = 536880467 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292218, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.37, 115.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701956 sequences (100000021 bp) in 2.358 CPU sec, 2.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292454, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 263, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (270.25, 90.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.48, 117.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159)
[M::mem_pestat] mean and std.dev: (313.29, 201.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1483)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702278 reads in 133.666 CPU sec, 4.261 real sec
[M::mem_process_seqs] Processed 701596 reads in 84.967 CPU sec, 2.680 real sec
[M::process] read 700558 sequences (100000065 bp) in 1.582 CPU sec, 1.625 real sec...
sorting block 71, #reads = 1095476 mem = 536880869 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292317, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.50, 115.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699688 sequences (100000220 bp) in 1.836 CPU sec, 1.952 real sec...
[M::process] read 699802 sequences (100000216 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291836, 8, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 259, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (215.11, 99.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (247.09, 119.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 511, 835)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1993)
[M::mem_pestat] mean and std.dev: (446.88, 242.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2572)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701956 reads in 137.152 CPU sec, 4.372 real sec
[M::mem_process_seqs] Processed 700558 reads in 85.994 CPU sec, 2.727 real sec
sorting block 72, #reads = 1095057 mem = 536877152 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291816, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.66, 119.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 241, 1161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3113)
[M::mem_pestat] mean and std.dev: (484.92, 435.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4089)
[M::mem_pestat] skip orientation RR
sorting block 73, #reads = 1092757 mem = 536878798 peak = 1152053248
[M::process] read 699346 sequences (100000064 bp) in 2.558 CPU sec, 2.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291765, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 200, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (239.09, 116.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (245.55, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699688 reads in 140.598 CPU sec, 4.466 real sec
[M::mem_process_seqs] Processed 699802 reads in 89.696 CPU sec, 2.822 real sec
[M::process] read 699312 sequences (100000272 bp) in 1.814 CPU sec, 1.849 real sec...
sorting block 74, #reads = 1091401 mem = 536880103 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291172, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (245.99, 117.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699288 sequences (100000113 bp) in 1.955 CPU sec, 2.056 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291527, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.37, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698908 sequences (100000233 bp) in 1.400 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 699346 reads in 140.923 CPU sec, 4.489 real sec
[M::mem_process_seqs] Processed 699312 reads in 88.714 CPU sec, 2.810 real sec
sorting block 75, #reads = 1091859 mem = 536879264 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291044, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.36, 118.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 711398 sequences (100000053 bp) in 2.315 CPU sec, 2.444 real sec...
[M::process] read 709626 sequences (100000000 bp) in 1.447 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290604, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.23, 118.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699288 reads in 149.371 CPU sec, 4.745 real sec
[M::mem_process_seqs] Processed 698908 reads in 97.962 CPU sec, 3.110 real sec
sorting block 76, #reads = 1089438 mem = 536876981 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 295308, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 197, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (218.99, 102.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708240 sequences (100000160 bp) in 1.939 CPU sec, 2.041 real sec...
[M::process] read 706480 sequences (100000078 bp) in 1.460 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 294400, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 200, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (223.69, 106.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 711398 reads in 149.575 CPU sec, 4.761 real sec
sorting block 77, #reads = 1083850 mem = 536880153 peak = 1152053248
[M::mem_process_seqs] Processed 709626 reads in 93.088 CPU sec, 2.954 real sec
sorting block 78, #reads = 1094819 mem = 536881119 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 293918, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (227.90, 108.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705288 sequences (100000184 bp) in 2.310 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293611, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (232.48, 111.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 278, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (285.55, 183.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1473)
[M::mem_pestat] skip orientation RR
[M::process] read 703952 sequences (100000215 bp) in 1.530 CPU sec, 1.532 real sec...
[M::mem_process_seqs] Processed 708240 reads in 147.284 CPU sec, 4.683 real sec
[M::mem_process_seqs] Processed 706480 reads in 92.981 CPU sec, 2.944 real sec
sorting block 79, #reads = 1093821 mem = 536879493 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293377, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (235.68, 113.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 777)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703726 sequences (100000111 bp) in 2.333 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292758, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (238.47, 115.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705288 reads in 143.085 CPU sec, 4.563 real sec
[M::mem_process_seqs] Processed 703952 reads in 90.653 CPU sec, 2.857 real sec
[M::process] read 703222 sequences (100000041 bp) in 1.804 CPU sec, 1.848 real sec...
sorting block 80, #reads = 1091772 mem = 536877872 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292604, 13, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.70, 115.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (60, 155, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328)
[M::mem_pestat] mean and std.dev: (413.85, 475.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3084)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 702670 sequences (100000023 bp) in 2.522 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292161, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (240.30, 116.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 198, 819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2139)
[M::mem_pestat] mean and std.dev: (376.91, 352.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2799)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 703726 reads in 143.349 CPU sec, 4.560 real sec
[M::process] read 700212 sequences (100000237 bp) in 1.591 CPU sec, 1.617 real sec...
[M::mem_process_seqs] Processed 703222 reads in 89.428 CPU sec, 2.822 real sec
sorting block 81, #reads = 1091764 mem = 536884926 peak = 1152053248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292193, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (238.40, 113.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 82, #reads = 1093012 mem = 536886067 peak = 1170006016
[M::process] read 699084 sequences (100000191 bp) in 2.117 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291655, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 850, 912)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2380)
[M::mem_pestat] mean and std.dev: (474.90, 340.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3114)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.03, 117.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699162 sequences (100000262 bp) in 1.470 CPU sec, 1.465 real sec...
[M::mem_process_seqs] Processed 702670 reads in 139.718 CPU sec, 4.447 real sec
[M::mem_process_seqs] Processed 700212 reads in 89.071 CPU sec, 2.813 real sec
sorting block 83, #reads = 1091396 mem = 536880210 peak = 1170006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291049, 4, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (247.13, 117.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 202, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (92, 302)
[M::mem_pestat] mean and std.dev: (190.07, 34.33)
[M::mem_pestat] low and high boundaries for proper pairs: (50, 344)
[M::mem_pestat] skip orientation RR
[M::process] read 698520 sequences (100000081 bp) in 2.517 CPU sec, 2.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291260, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (246.16, 116.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699084 reads in 136.830 CPU sec, 4.366 real sec
[M::mem_process_seqs] Processed 699162 reads in 87.598 CPU sec, 2.768 real sec
[M::process] read 697696 sequences (100000155 bp) in 1.772 CPU sec, 1.815 real sec...
sorting block 84, #reads = 1090248 mem = 536877951 peak = 1170006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290830, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (246.45, 115.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 333, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (325.20, 204.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1407)
[M::mem_pestat] skip orientation RR
[M::process] read 697824 sequences (100000115 bp) in 1.954 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290431, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (247.95, 116.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 212, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (214.00, 66.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698520 reads in 136.327 CPU sec, 4.344 real sec
[M::process] read 697318 sequences (100000298 bp) in 1.541 CPU sec, 1.548 real sec...
[M::mem_process_seqs] Processed 697696 reads in 86.466 CPU sec, 2.729 real sec
sorting block 85, #reads = 1091522 mem = 536882973 peak = 1170006016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290662, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (250.01, 119.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 282488 sequences (40460656 bp) in 1.260 CPU sec, 1.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290211, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.60, 120.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697824 reads in 131.005 CPU sec, 4.242 real sec
sorting block 86, #reads = 1090814 mem = 536881498 peak = 1170006016
[M::mem_process_seqs] Processed 697318 reads in 82.127 CPU sec, 2.664 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 117715, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.48, 120.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 282488 reads in 32.328 CPU sec, 1.088 real sec
sorting block 87, #reads = 1091148 mem = 536885369 peak = 1268494336
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96274254
overall: 70089023488 mem 11421.222 user 333.070 sys 625.698 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 625.740 sec; CPU: 11754.295 sec
sorting block 88, #reads = 550184 mem = 271161182 peak = 1268494336
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5229284158
output reads: 96765474
bam_mem_sort: 89 calls 35.393 user 0.770 sys 36.327 real
bam_write: 89 calls 45.167 user 1.399 sys 46.941 real
execute: 1 calls 19.244 user 1.050 sys 653.322 real
merge_files: 1 calls 19.232 user 1.021 sys 26.885 real
parse_chunk: 1417 calls 45.554 user 13.087 sys 59.746 real
read_chunk: 3827 calls 34.544 user 34.867 sys 342.165 real
sort_block: 1 calls 27.397 user 34.319 sys 330.926 real
write_chunk: 2408 calls 18.849 user 0.776 sys 26.031 real
overall: 3036602368 mem 1054.054 user 59.232 sys 653.356 real
ls: cannot access '*.cram': No such file or directory