Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cb/620b4da73384960f9da29a98f5d645/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/73/a50ab7fac9266b337d7ac430cc87f8/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cb/620b4da73384960f9da29a98f5d645/.command.run
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/73/a50ab7fac9266b337d7ac430cc87f8/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1643.979 CPU sec, 535.867 real sec
[M::process] read 702988 sequences (100000191 bp) in 0.972 CPU sec, 0.978 real sec...
[M::process] read 701418 sequences (100000140 bp) in 1.187 CPU sec, 1.220 real sec...
[M::process] read 700212 sequences (100000204 bp) in 1.200 CPU sec, 1.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292225, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (238.97, 113.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699766 sequences (100000111 bp) in 1.157 CPU sec, 1.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291797, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.87, 115.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292007, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (245.52, 116.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702988 reads in 218.590 CPU sec, 7.013 real sec
[M::mem_process_seqs] Processed 701418 reads in 139.601 CPU sec, 4.517 real sec
[M::mem_process_seqs] Processed 700212 reads in 150.869 CPU sec, 4.899 real sec
sorting block 0, #reads = 1095167 mem = 536877344 peak = 604766208
[M::process] read 699162 sequences (100000219 bp) in 1.678 CPU sec, 1.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291487, 9, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 216, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 417)
[M::mem_pestat] mean and std.dev: (208.00, 46.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.07, 117.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 541, 810)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898)
[M::mem_pestat] mean and std.dev: (438.36, 225.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2442)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698510 sequences (100000088 bp) in 1.368 CPU sec, 1.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290853, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.39, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 275, 644)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1552)
[M::mem_pestat] mean and std.dev: (357.62, 241.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2006)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699766 reads in 131.184 CPU sec, 4.198 real sec
[M::process] read 698024 sequences (100000272 bp) in 1.263 CPU sec, 1.265 real sec...
sorting block 1, #reads = 1095217 mem = 536878880 peak = 851185664
[M::mem_process_seqs] Processed 699162 reads in 83.434 CPU sec, 2.668 real sec
sorting block 2, #reads = 1094818 mem = 536876827 peak = 890941440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291240, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (164.22, 38.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.79, 120.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698070 sequences (100000199 bp) in 1.720 CPU sec, 1.820 real sec...
[M::process] read 697866 sequences (100000065 bp) in 1.240 CPU sec, 1.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291205, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (251.87, 120.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698510 reads in 128.175 CPU sec, 4.079 real sec
[M::mem_process_seqs] Processed 698024 reads in 79.977 CPU sec, 2.547 real sec
sorting block 3, #reads = 1094271 mem = 536877576 peak = 890941440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290587, 8, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 232, 2088)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5860)
[M::mem_pestat] mean and std.dev: (817.00, 995.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7746)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.92, 120.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 416, 2297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6407)
[M::mem_pestat] mean and std.dev: (828.25, 902.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8462)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697874 sequences (100000251 bp) in 1.699 CPU sec, 1.806 real sec...
[M::process] read 697878 sequences (100000242 bp) in 1.230 CPU sec, 1.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290828, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.19, 119.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698070 reads in 128.896 CPU sec, 4.106 real sec
[M::mem_process_seqs] Processed 697866 reads in 80.620 CPU sec, 2.570 real sec
sorting block 4, #reads = 1094183 mem = 536880428 peak = 890941440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290621, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (249.17, 118.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697508 sequences (100000210 bp) in 1.753 CPU sec, 1.893 real sec...
[M::process] read 697142 sequences (100000002 bp) in 1.260 CPU sec, 1.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290571, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 184, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (186.00, 59.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (249.67, 118.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697874 reads in 129.940 CPU sec, 4.145 real sec
sorting block 5, #reads = 1093020 mem = 536876940 peak = 890941440
[M::mem_process_seqs] Processed 697878 reads in 81.805 CPU sec, 2.600 real sec
sorting block 6, #reads = 1093393 mem = 536881099 peak = 890941440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291025, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.45, 119.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696030 sequences (100000008 bp) in 1.962 CPU sec, 2.067 real sec...
[M::process] read 696418 sequences (100000235 bp) in 1.300 CPU sec, 1.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290374, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.29, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697508 reads in 129.697 CPU sec, 4.136 real sec
[M::mem_process_seqs] Processed 697142 reads in 81.501 CPU sec, 2.597 real sec
sorting block 7, #reads = 1092964 mem = 536879108 peak = 890941440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 289902, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.66, 120.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695810 sequences (100000278 bp) in 1.786 CPU sec, 1.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289937, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (384, 824, 1041)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2355)
[M::mem_pestat] mean and std.dev: (858.10, 642.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3428)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.69, 119.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695784 sequences (100000110 bp) in 1.330 CPU sec, 1.324 real sec...
[M::mem_process_seqs] Processed 696030 reads in 129.481 CPU sec, 4.132 real sec
[M::mem_process_seqs] Processed 696418 reads in 81.540 CPU sec, 2.591 real sec
sorting block 8, #reads = 1092785 mem = 536878324 peak = 890941440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290136, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (253.39, 118.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695076 sequences (100000191 bp) in 2.137 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290499, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (254.72, 119.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698536 sequences (100000040 bp) in 1.380 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 695810 reads in 129.715 CPU sec, 4.131 real sec
[M::mem_process_seqs] Processed 695784 reads in 81.547 CPU sec, 2.589 real sec
sorting block 9, #reads = 1091844 mem = 536880634 peak = 890941440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290242, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 234, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (257.18, 121.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701656 sequences (100000291 bp) in 1.947 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290542, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 1143)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3027)
[M::mem_pestat] mean and std.dev: (430.22, 386.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3969)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (248.96, 118.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 695076 reads in 130.146 CPU sec, 4.157 real sec
[M::process] read 700812 sequences (100000003 bp) in 1.389 CPU sec, 1.393 real sec...
sorting block 10, #reads = 1092776 mem = 536877950 peak = 890941440
[M::mem_process_seqs] Processed 698536 reads in 83.373 CPU sec, 2.639 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292016, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.20, 116.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1092516 mem = 536879319 peak = 911486976
[M::process] read 700210 sequences (100000054 bp) in 2.264 CPU sec, 2.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291862, 11, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.21, 117.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 571, 860)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2532)
[M::mem_pestat] mean and std.dev: (436.30, 384.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3368)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 701656 reads in 130.116 CPU sec, 4.142 real sec
[M::process] read 699450 sequences (100000077 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_process_seqs] Processed 700812 reads in 82.231 CPU sec, 2.604 real sec
sorting block 12, #reads = 1097069 mem = 536879658 peak = 911486976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291553, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.19, 118.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 340, 2236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6224)
[M::mem_pestat] mean and std.dev: (901.15, 906.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8218)
[M::mem_pestat] skip orientation RR
[M::process] read 699358 sequences (100000135 bp) in 2.085 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291057, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.40, 120.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700210 reads in 129.789 CPU sec, 4.153 real sec
[M::mem_process_seqs] Processed 699450 reads in 82.378 CPU sec, 2.603 real sec
[M::process] read 699020 sequences (100000020 bp) in 1.651 CPU sec, 1.708 real sec...
sorting block 13, #reads = 1095367 mem = 536878127 peak = 911486976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291352, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 278, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1537)
[M::mem_pestat] mean and std.dev: (286.33, 189.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1992)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.19, 120.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (460, 1044, 1626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3958)
[M::mem_pestat] mean and std.dev: (886.30, 592.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5124)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698394 sequences (100000110 bp) in 2.162 CPU sec, 2.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291544, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.58, 121.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699358 reads in 129.509 CPU sec, 4.133 real sec
[M::process] read 697844 sequences (100000076 bp) in 1.541 CPU sec, 1.575 real sec...
[M::mem_process_seqs] Processed 699020 reads in 82.185 CPU sec, 2.599 real sec
sorting block 14, #reads = 1095058 mem = 536877755 peak = 911486976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290746, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 155, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (198.22, 108.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.86, 121.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 15, #reads = 1095468 mem = 536877693 peak = 911486976
[M::process] read 698006 sequences (100000086 bp) in 2.107 CPU sec, 2.295 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290220, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 336, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (257.00, 125.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (251.93, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698394 reads in 130.470 CPU sec, 4.180 real sec
[M::mem_process_seqs] Processed 697844 reads in 83.194 CPU sec, 2.633 real sec
[M::process] read 698372 sequences (100000109 bp) in 1.797 CPU sec, 1.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290875, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.41, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1094090 mem = 536877379 peak = 911486976
[M::process] read 697966 sequences (100000251 bp) in 1.938 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290945, 6, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 200, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (212.44, 63.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 633)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.67, 118.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698006 reads in 131.981 CPU sec, 4.225 real sec
[M::mem_process_seqs] Processed 698372 reads in 83.614 CPU sec, 2.642 real sec
[M::process] read 697572 sequences (100000261 bp) in 1.614 CPU sec, 1.665 real sec...
sorting block 17, #reads = 1092877 mem = 536878162 peak = 911486976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290632, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 283, 846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2246)
[M::mem_pestat] mean and std.dev: (450.45, 399.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2946)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.52, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697376 sequences (100000111 bp) in 2.089 CPU sec, 2.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290726, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (249.82, 118.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697966 reads in 130.514 CPU sec, 4.176 real sec
[M::mem_process_seqs] Processed 697572 reads in 82.629 CPU sec, 2.616 real sec
[M::process] read 697420 sequences (100000238 bp) in 1.694 CPU sec, 1.764 real sec...
sorting block 18, #reads = 1094622 mem = 536881215 peak = 911486976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290583, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.52, 118.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696602 sequences (100000113 bp) in 1.964 CPU sec, 2.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290501, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.66, 120.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697376 reads in 131.103 CPU sec, 4.190 real sec
sorting block 19, #reads = 1093603 mem = 536883225 peak = 911486976
[M::mem_process_seqs] Processed 697420 reads in 83.316 CPU sec, 2.633 real sec
[M::process] read 696406 sequences (100000064 bp) in 1.540 CPU sec, 1.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290454, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 177, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295)
[M::mem_pestat] mean and std.dev: (204.90, 132.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1683)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.89, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 302, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (305.15, 96.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 20, #reads = 1093399 mem = 536878904 peak = 953294848
[M::process] read 695596 sequences (100000247 bp) in 2.021 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290607, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.72, 119.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468)
[M::mem_pestat] mean and std.dev: (213.10, 88.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 569)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696602 reads in 131.191 CPU sec, 4.196 real sec
[M::process] read 696078 sequences (100000071 bp) in 1.549 CPU sec, 1.561 real sec...
[M::mem_process_seqs] Processed 696406 reads in 83.182 CPU sec, 2.627 real sec
sorting block 21, #reads = 1092219 mem = 536878322 peak = 953294848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 289804, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (255.02, 120.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702184 sequences (100000093 bp) in 2.092 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290082, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 190, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372)
[M::mem_pestat] mean and std.dev: (160.88, 32.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 451)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.55, 122.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 695596 reads in 131.335 CPU sec, 4.195 real sec
[M::process] read 701092 sequences (100000136 bp) in 1.469 CPU sec, 1.475 real sec...
[M::mem_process_seqs] Processed 696078 reads in 83.603 CPU sec, 2.646 real sec
sorting block 22, #reads = 1093007 mem = 536884532 peak = 953294848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292131, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.39, 114.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 233, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (226.85, 82.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] skip orientation RR
[M::process] read 700422 sequences (100000031 bp) in 2.003 CPU sec, 2.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291804, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.69, 115.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699654 sequences (100000229 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 702184 reads in 133.444 CPU sec, 4.261 real sec
[M::mem_process_seqs] Processed 701092 reads in 84.258 CPU sec, 2.672 real sec
sorting block 23, #reads = 1093144 mem = 536876460 peak = 953294848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291228, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.27, 117.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1095187 mem = 536876909 peak = 953294848
[M::process] read 698888 sequences (100000293 bp) in 2.332 CPU sec, 2.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291486, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.18, 117.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700422 reads in 132.498 CPU sec, 4.222 real sec
[M::process] read 698678 sequences (100000199 bp) in 1.446 CPU sec, 1.456 real sec...
[M::mem_process_seqs] Processed 699654 reads in 83.810 CPU sec, 2.651 real sec
sorting block 25, #reads = 1093888 mem = 536883510 peak = 953294848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291397, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 263, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (217.50, 96.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.21, 118.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 220, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (207.70, 56.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697754 sequences (100000139 bp) in 2.005 CPU sec, 2.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291095, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.71, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698888 reads in 131.273 CPU sec, 4.192 real sec
[M::process] read 697690 sequences (100000126 bp) in 1.427 CPU sec, 1.437 real sec...
[M::mem_process_seqs] Processed 698678 reads in 82.911 CPU sec, 2.623 real sec
sorting block 26, #reads = 1093587 mem = 536878970 peak = 953294848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290980, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.74, 120.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697832 sequences (100000076 bp) in 2.362 CPU sec, 2.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290850, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.79, 121.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697754 reads in 129.978 CPU sec, 4.140 real sec
[M::process] read 697570 sequences (100000299 bp) in 1.531 CPU sec, 1.559 real sec...
[M::mem_process_seqs] Processed 697690 reads in 82.137 CPU sec, 2.595 real sec
sorting block 27, #reads = 1094090 mem = 536879438 peak = 953294848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290631, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.12, 120.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 234, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (221.33, 77.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 622)
[M::mem_pestat] skip orientation RR
[M::process] read 697860 sequences (100000219 bp) in 2.052 CPU sec, 2.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291007, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.27, 120.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697832 reads in 130.669 CPU sec, 4.174 real sec
[M::mem_process_seqs] Processed 697570 reads in 82.457 CPU sec, 2.602 real sec
sorting block 28, #reads = 1094228 mem = 536878453 peak = 953294848
[M::process] read 697562 sequences (100000016 bp) in 1.557 CPU sec, 1.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290487, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (249.80, 118.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (343, 352, 1213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2953)
[M::mem_pestat] mean and std.dev: (861.45, 803.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4074)
[M::mem_pestat] skip orientation RR
sorting block 29, #reads = 1093859 mem = 536881019 peak = 994574336
[M::process] read 697358 sequences (100000042 bp) in 2.239 CPU sec, 2.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290472, 10, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 187, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 352)
[M::mem_pestat] mean and std.dev: (167.67, 35.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 419)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.15, 118.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 362, 7524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22298)
[M::mem_pestat] mean and std.dev: (2411.60, 3348.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 29685)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (594, 1077, 1699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3909)
[M::mem_pestat] mean and std.dev: (956.22, 538.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5014)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697860 reads in 130.026 CPU sec, 4.145 real sec
[M::process] read 697340 sequences (100000296 bp) in 1.495 CPU sec, 1.505 real sec...
[M::mem_process_seqs] Processed 697562 reads in 82.614 CPU sec, 2.611 real sec
sorting block 30, #reads = 1093362 mem = 536881963 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290220, 10, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.65, 119.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 141, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328)
[M::mem_pestat] mean and std.dev: (188.22, 202.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1760)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 253, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (252.67, 83.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696992 sequences (100000160 bp) in 1.989 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290560, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.64, 119.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697358 reads in 128.735 CPU sec, 4.107 real sec
[M::process] read 696694 sequences (100000297 bp) in 1.481 CPU sec, 1.506 real sec...
[M::mem_process_seqs] Processed 697340 reads in 81.714 CPU sec, 2.577 real sec
sorting block 31, #reads = 1094236 mem = 536877960 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289966, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 419, 839)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2101)
[M::mem_pestat] mean and std.dev: (664.64, 582.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2996)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.24, 119.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696312 sequences (100000016 bp) in 2.338 CPU sec, 2.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290811, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.15, 120.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696992 reads in 129.098 CPU sec, 4.110 real sec
[M::mem_process_seqs] Processed 696694 reads in 81.145 CPU sec, 2.562 real sec
[M::process] read 695580 sequences (100000165 bp) in 1.585 CPU sec, 1.625 real sec...
sorting block 32, #reads = 1093189 mem = 536876876 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289933, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 242, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (210.08, 77.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 692)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.72, 121.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 33, #reads = 1092824 mem = 536876555 peak = 994574336
[M::process] read 700048 sequences (100000062 bp) in 2.315 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289620, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (256.37, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696312 reads in 126.107 CPU sec, 4.041 real sec
[M::mem_process_seqs] Processed 695580 reads in 80.903 CPU sec, 2.560 real sec
[M::process] read 702058 sequences (100000238 bp) in 2.088 CPU sec, 2.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291514, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (245.76, 117.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1092842 mem = 536880065 peak = 994574336
[M::process] read 701056 sequences (100000247 bp) in 1.938 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292485, 9, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 478, 829)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085)
[M::mem_pestat] mean and std.dev: (383.38, 275.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.23, 116.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700048 reads in 126.971 CPU sec, 4.057 real sec
[M::mem_process_seqs] Processed 702058 reads in 80.633 CPU sec, 2.548 real sec
[M::process] read 700834 sequences (100000116 bp) in 1.950 CPU sec, 2.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291981, 10, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 163, 167)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 253)
[M::mem_pestat] mean and std.dev: (144.67, 35.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 296)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.97, 118.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1202, 1309, 1309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (988, 1523)
[M::mem_pestat] mean and std.dev: (1293.71, 37.44)
[M::mem_pestat] low and high boundaries for proper pairs: (881, 1630)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
sorting block 35, #reads = 1093125 mem = 536877657 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291658, 6, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.61, 118.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 366, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (369.23, 195.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701056 reads in 125.309 CPU sec, 4.029 real sec
[M::process] read 700208 sequences (100000229 bp) in 2.518 CPU sec, 2.716 real sec...
[M::mem_process_seqs] Processed 700834 reads in 80.523 CPU sec, 2.562 real sec
sorting block 36, #reads = 1095715 mem = 536886299 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291317, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (347, 369, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (223, 533)
[M::mem_pestat] mean and std.dev: (366.71, 44.20)
[M::mem_pestat] low and high boundaries for proper pairs: (161, 595)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.20, 119.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700208 reads in 69.469 CPU sec, 2.320 real sec
[M::process] read 699578 sequences (100000155 bp) in 2.647 CPU sec, 2.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291106, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.37, 119.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1095140 mem = 536881774 peak = 994574336
[M::mem_process_seqs] Processed 699578 reads in 68.629 CPU sec, 2.281 real sec
[M::process] read 699800 sequences (100000116 bp) in 2.753 CPU sec, 2.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291273, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.51, 119.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 276, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478)
[M::mem_pestat] mean and std.dev: (312.75, 197.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1915)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699800 reads in 69.490 CPU sec, 2.287 real sec
sorting block 38, #reads = 1095076 mem = 536887067 peak = 994574336
[M::process] read 698762 sequences (100000236 bp) in 2.702 CPU sec, 2.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290994, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 277, 1122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3040)
[M::mem_pestat] mean and std.dev: (558.46, 564.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3999)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.66, 120.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 140, 710)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1884)
[M::mem_pestat] mean and std.dev: (258.11, 241.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2471)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698762 reads in 68.444 CPU sec, 2.225 real sec
[M::process] read 698102 sequences (100000097 bp) in 2.683 CPU sec, 2.914 real sec...
sorting block 39, #reads = 1094926 mem = 536877611 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290833, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.80, 121.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (332, 379, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (212, 512)
[M::mem_pestat] mean and std.dev: (377.14, 20.52)
[M::mem_pestat] low and high boundaries for proper pairs: (152, 572)
[M::mem_pestat] skip orientation RR
[M::process] read 697872 sequences (100000120 bp) in 2.140 CPU sec, 2.253 real sec...
[M::mem_process_seqs] Processed 698102 reads in 69.648 CPU sec, 2.255 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290555, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.28, 120.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697872 reads in 68.716 CPU sec, 2.226 real sec
sorting block 40, #reads = 1094440 mem = 536879395 peak = 994574336
[M::process] read 698062 sequences (100000161 bp) in 2.449 CPU sec, 2.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290787, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 256, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (199.50, 68.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 736)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.40, 119.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697656 sequences (100000195 bp) in 2.050 CPU sec, 2.155 real sec...
[M::mem_process_seqs] Processed 698062 reads in 69.143 CPU sec, 2.239 real sec
sorting block 41, #reads = 1094769 mem = 536879079 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290874, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.49, 119.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697656 reads in 69.552 CPU sec, 2.248 real sec
[M::process] read 697686 sequences (100000068 bp) in 2.416 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290258, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.01, 118.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (293, 337, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238)
[M::mem_pestat] mean and std.dev: (370.67, 136.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1553)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697686 reads in 68.786 CPU sec, 2.228 real sec
[M::process] read 697418 sequences (100000068 bp) in 2.115 CPU sec, 2.234 real sec...
sorting block 42, #reads = 1092783 mem = 536879757 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290430, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.38, 118.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697418 reads in 70.048 CPU sec, 2.272 real sec
[M::process] read 696848 sequences (100000205 bp) in 2.583 CPU sec, 2.759 real sec...
sorting block 43, #reads = 1093790 mem = 536876616 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290548, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 325, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338)
[M::mem_pestat] mean and std.dev: (360.10, 256.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.55, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696848 reads in 68.845 CPU sec, 2.230 real sec
[M::process] read 696378 sequences (100000157 bp) in 2.152 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290811, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.68, 119.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696378 reads in 68.367 CPU sec, 2.218 real sec
sorting block 44, #reads = 1093654 mem = 536882630 peak = 994574336
[M::process] read 696306 sequences (100000147 bp) in 2.592 CPU sec, 2.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290341, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.05, 119.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695746 sequences (100000015 bp) in 1.961 CPU sec, 2.072 real sec...
[M::mem_process_seqs] Processed 696306 reads in 68.952 CPU sec, 2.228 real sec
sorting block 45, #reads = 1091884 mem = 536876597 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290329, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.75, 121.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699888 sequences (100000201 bp) in 1.930 CPU sec, 2.056 real sec...
[M::mem_process_seqs] Processed 695746 reads in 69.284 CPU sec, 2.232 real sec
[M::process] read 706382 sequences (100000032 bp) in 1.963 CPU sec, 2.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291371, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.88, 118.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704644 sequences (100000043 bp) in 1.240 CPU sec, 1.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293454, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 283, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (251.40, 90.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.63, 109.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699888 reads in 129.073 CPU sec, 4.060 real sec
[M::process] read 703484 sequences (100000197 bp) in 1.376 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 706382 reads in 82.060 CPU sec, 2.580 real sec
sorting block 46, #reads = 1092213 mem = 536884315 peak = 994574336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293138, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.37, 112.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 321, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (265.75, 95.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] skip orientation RR
sorting block 47, #reads = 1095723 mem = 536878618 peak = 1024442368
[M::process] read 702680 sequences (100000208 bp) in 2.433 CPU sec, 2.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292973, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 203, 686)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1736)
[M::mem_pestat] mean and std.dev: (355.42, 300.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2261)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.77, 114.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 704644 reads in 128.921 CPU sec, 4.107 real sec
[M::mem_process_seqs] Processed 703484 reads in 81.924 CPU sec, 2.589 real sec
[M::process] read 702254 sequences (100000065 bp) in 1.742 CPU sec, 1.819 real sec...
sorting block 48, #reads = 1097860 mem = 536886074 peak = 1024442368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292874, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.62, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701216 sequences (100000043 bp) in 2.007 CPU sec, 2.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292412, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 205, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131)
[M::mem_pestat] mean and std.dev: (291.36, 148.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1441)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.20, 118.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 279, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (319.85, 176.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 700626 sequences (100000126 bp) in 1.300 CPU sec, 1.306 real sec...
[M::mem_process_seqs] Processed 702680 reads in 132.508 CPU sec, 4.209 real sec
[M::mem_process_seqs] Processed 702254 reads in 81.578 CPU sec, 2.584 real sec
sorting block 49, #reads = 1095379 mem = 536875916 peak = 1024442368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291759, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.21, 120.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699692 sequences (100000153 bp) in 2.345 CPU sec, 2.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291984, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.61, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701216 reads in 128.118 CPU sec, 4.071 real sec
[M::mem_process_seqs] Processed 700626 reads in 81.940 CPU sec, 2.582 real sec
[M::process] read 699422 sequences (100000030 bp) in 1.597 CPU sec, 1.644 real sec...
sorting block 50, #reads = 1095615 mem = 536885718 peak = 1024442368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291889, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.45, 121.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698528 sequences (100000018 bp) in 2.130 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291371, 10, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 395, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1119)
[M::mem_pestat] mean and std.dev: (368.70, 197.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1423)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (249.29, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 82, 1024)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3018)
[M::mem_pestat] mean and std.dev: (284.75, 412.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4015)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 699692 reads in 129.802 CPU sec, 4.128 real sec
sorting block 51, #reads = 1094176 mem = 536879025 peak = 1024442368
[M::process] read 698648 sequences (100000263 bp) in 1.435 CPU sec, 1.433 real sec...
[M::mem_process_seqs] Processed 699422 reads in 82.906 CPU sec, 2.616 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290822, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 52, #reads = 1093811 mem = 536883368 peak = 1040826368
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.36, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 250, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (239.00, 64.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RR
[M::process] read 698614 sequences (100000072 bp) in 2.397 CPU sec, 2.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291054, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 276, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1255)
[M::mem_pestat] mean and std.dev: (321.20, 207.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1623)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.48, 120.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698528 reads in 130.255 CPU sec, 4.142 real sec
[M::mem_process_seqs] Processed 698648 reads in 83.613 CPU sec, 2.634 real sec
[M::process] read 697888 sequences (100000042 bp) in 1.740 CPU sec, 1.803 real sec...
sorting block 53, #reads = 1091609 mem = 536877953 peak = 1040826368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290692, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 323, 2191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6281)
[M::mem_pestat] mean and std.dev: (865.23, 966.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8326)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.19, 120.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697706 sequences (100000137 bp) in 2.238 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290426, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 397, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232)
[M::mem_pestat] mean and std.dev: (327.70, 183.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1587)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.63, 121.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 271, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (261.64, 108.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698614 reads in 132.059 CPU sec, 4.202 real sec
[M::mem_process_seqs] Processed 697888 reads in 85.082 CPU sec, 2.681 real sec
[M::process] read 697504 sequences (100000174 bp) in 1.736 CPU sec, 1.794 real sec...
sorting block 54, #reads = 1091971 mem = 536889496 peak = 1040826368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290438, 13, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 244, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (290.36, 155.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1334)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.35, 119.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 365, 1210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3076)
[M::mem_pestat] mean and std.dev: (837.00, 941.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4603)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 697404 sequences (100000251 bp) in 2.531 CPU sec, 2.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290607, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 361, 1531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4133)
[M::mem_pestat] mean and std.dev: (879.70, 1023.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5434)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.79, 118.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 430, 851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2049)
[M::mem_pestat] mean and std.dev: (478.30, 288.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2648)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697706 reads in 130.740 CPU sec, 4.170 real sec
[M::mem_process_seqs] Processed 697504 reads in 83.639 CPU sec, 2.639 real sec
sorting block 55, #reads = 1091046 mem = 536882277 peak = 1040826368
[M::process] read 698002 sequences (100000143 bp) in 2.038 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290336, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.68, 119.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1091419 mem = 536881222 peak = 1040826368
[M::process] read 707398 sequences (100000294 bp) in 2.252 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290529, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.22, 119.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697404 reads in 134.054 CPU sec, 4.273 real sec
[M::mem_process_seqs] Processed 698002 reads in 86.169 CPU sec, 2.719 real sec
[M::process] read 706122 sequences (100000265 bp) in 2.083 CPU sec, 2.202 real sec...
sorting block 57, #reads = 1090181 mem = 536879858 peak = 1040826368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293236, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 203, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (226.80, 107.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704768 sequences (100000066 bp) in 2.330 CPU sec, 2.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293206, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.34, 109.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707398 reads in 139.634 CPU sec, 4.449 real sec
[M::mem_process_seqs] Processed 706122 reads in 88.250 CPU sec, 2.785 real sec
[M::process] read 704280 sequences (100000162 bp) in 2.045 CPU sec, 2.160 real sec...
sorting block 58, #reads = 1092015 mem = 536878597 peak = 1040826368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293158, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 211, 1503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4093)
[M::mem_pestat] mean and std.dev: (399.67, 419.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5388)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.42, 112.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 209, 1928)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5420)
[M::mem_pestat] mean and std.dev: (816.00, 910.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7166)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 704490 sequences (100000282 bp) in 2.408 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292976, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.13, 112.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704768 reads in 135.169 CPU sec, 4.315 real sec
[M::mem_process_seqs] Processed 704280 reads in 86.990 CPU sec, 2.752 real sec
sorting block 59, #reads = 1095155 mem = 536879698 peak = 1040826368
[M::process] read 702870 sequences (100000115 bp) in 2.151 CPU sec, 2.277 real sec...
sorting block 60, #reads = 1094787 mem = 536878294 peak = 1040826368
[M::process] read 701944 sequences (100000050 bp) in 2.233 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292990, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.63, 115.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702870 reads in 74.021 CPU sec, 2.341 real sec
[M::process] read 701442 sequences (100000206 bp) in 1.951 CPU sec, 2.061 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293129, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.40, 112.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291983, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.62, 116.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704490 reads in 210.881 CPU sec, 6.707 real sec
[M::mem_process_seqs] Processed 701944 reads in 99.314 CPU sec, 3.142 real sec
sorting block 61, #reads = 1094632 mem = 536877633 peak = 1040826368
[M::process] read 700320 sequences (100000027 bp) in 2.240 CPU sec, 2.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292016, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.89, 118.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699448 sequences (100000127 bp) in 2.302 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291716, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.41, 120.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701442 reads in 134.411 CPU sec, 4.288 real sec
sorting block 62, #reads = 1091850 mem = 536879129 peak = 1040826368
[M::mem_process_seqs] Processed 700320 reads in 86.510 CPU sec, 2.737 real sec
[M::process] read 699342 sequences (100000202 bp) in 2.142 CPU sec, 2.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291553, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.62, 120.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1092810 mem = 536879829 peak = 1040826368
[M::process] read 698664 sequences (100000090 bp) in 2.074 CPU sec, 2.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291566, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (282, 600, 1609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4263)
[M::mem_pestat] mean and std.dev: (748.31, 625.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5590)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.73, 120.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699448 reads in 134.361 CPU sec, 4.295 real sec
[M::mem_process_seqs] Processed 699342 reads in 85.835 CPU sec, 2.713 real sec
[M::process] read 698774 sequences (100000056 bp) in 2.221 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291151, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.96, 120.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 64, #reads = 1092594 mem = 536877519 peak = 1040826368
[M::process] read 698722 sequences (100000025 bp) in 2.161 CPU sec, 2.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290709, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.57, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 233, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (331.43, 208.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698664 reads in 132.453 CPU sec, 4.234 real sec
[M::mem_process_seqs] Processed 698774 reads in 85.000 CPU sec, 2.691 real sec
sorting block 65, #reads = 1091509 mem = 536882251 peak = 1040826368
[M::process] read 697776 sequences (100000000 bp) in 2.217 CPU sec, 2.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291395, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 266, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 398)
[M::mem_pestat] mean and std.dev: (212.62, 50.24)
[M::mem_pestat] low and high boundaries for proper pairs: (2, 464)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.54, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698208 sequences (100000156 bp) in 2.086 CPU sec, 2.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290504, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.64, 121.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698722 reads in 131.618 CPU sec, 4.220 real sec
[M::mem_process_seqs] Processed 697776 reads in 84.441 CPU sec, 2.676 real sec
sorting block 66, #reads = 1092430 mem = 536878836 peak = 1040826368
[M::process] read 701306 sequences (100000205 bp) in 2.361 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290506, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.40, 122.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1091293 mem = 536882314 peak = 1040826368
[M::process] read 705482 sequences (100000066 bp) in 2.292 CPU sec, 2.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291286, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 293, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282)
[M::mem_pestat] mean and std.dev: (336.78, 227.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1658)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (242.13, 117.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698208 reads in 143.399 CPU sec, 4.582 real sec
[M::mem_process_seqs] Processed 701306 reads in 96.756 CPU sec, 3.062 real sec
[M::process] read 703682 sequences (100000037 bp) in 2.421 CPU sec, 2.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293556, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 248, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (233.44, 110.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (232.91, 111.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 354, 951)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2497)
[M::mem_pestat] mean and std.dev: (469.00, 346.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3270)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 68, #reads = 1089693 mem = 536878393 peak = 1040826368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292869, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (237.04, 113.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 200, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486)
[M::mem_pestat] mean and std.dev: (345.00, 347.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1981)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 703050 sequences (100000178 bp) in 2.169 CPU sec, 2.487 real sec...
[M::mem_process_seqs] Processed 705482 reads in 130.169 CPU sec, 4.171 real sec
[M::mem_process_seqs] Processed 703682 reads in 82.841 CPU sec, 2.631 real sec
sorting block 69, #reads = 1091705 mem = 536878555 peak = 1040826368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292763, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.95, 115.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702788 sequences (100000184 bp) in 2.514 CPU sec, 2.643 real sec...
[M::mem_process_seqs] Processed 703050 reads in 72.060 CPU sec, 2.388 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292810, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.15, 114.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702788 reads in 72.670 CPU sec, 2.395 real sec
sorting block 70, #reads = 1096891 mem = 536876873 peak = 1040826368
[M::process] read 701680 sequences (100000184 bp) in 2.471 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292226, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.30, 117.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701680 reads in 71.368 CPU sec, 2.350 real sec
[M::process] read 702042 sequences (100000031 bp) in 2.503 CPU sec, 2.734 real sec...
sorting block 71, #reads = 1094366 mem = 536880474 peak = 1040826368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292365, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (242.46, 118.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702042 reads in 74.488 CPU sec, 2.428 real sec
[M::process] read 701554 sequences (100000149 bp) in 2.496 CPU sec, 2.710 real sec...
sorting block 72, #reads = 1095400 mem = 536881500 peak = 1040826368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292352, 12, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.53, 118.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 223, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (160.70, 98.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 701100 sequences (100000137 bp) in 2.258 CPU sec, 2.414 real sec...
[M::mem_process_seqs] Processed 701554 reads in 76.647 CPU sec, 2.477 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292185, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 334, 799)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2079)
[M::mem_pestat] mean and std.dev: (411.90, 308.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2719)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.37, 118.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700400 sequences (100000188 bp) in 2.080 CPU sec, 2.209 real sec...
[M::mem_process_seqs] Processed 701100 reads in 71.170 CPU sec, 2.299 real sec
sorting block 73, #reads = 1094616 mem = 536879757 peak = 1040826368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291618, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 256, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (292.43, 152.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.75, 119.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698988 sequences (100000003 bp) in 2.101 CPU sec, 2.220 real sec...
[M::mem_process_seqs] Processed 700400 reads in 72.580 CPU sec, 2.359 real sec
sorting block 74, #reads = 1093882 mem = 536880888 peak = 1040826368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290814, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 1120)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3022)
[M::mem_pestat] mean and std.dev: (416.08, 409.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3973)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (250.02, 121.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698740 sequences (100000299 bp) in 2.199 CPU sec, 2.350 real sec...
[M::mem_process_seqs] Processed 698988 reads in 74.558 CPU sec, 2.402 real sec
[M::process] read 698482 sequences (100000098 bp) in 2.112 CPU sec, 2.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290755, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (250.12, 121.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698972 sequences (100000291 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291035, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.44, 120.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698740 reads in 130.002 CPU sec, 4.097 real sec
sorting block 75, #reads = 1091472 mem = 536883338 peak = 1040826368
[M::process] read 698410 sequences (100000068 bp) in 1.908 CPU sec, 2.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290994, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.12, 119.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698972 reads in 74.088 CPU sec, 2.341 real sec
[M::process] read 698168 sequences (100000063 bp) in 1.922 CPU sec, 1.994 real sec...
sorting block 76, #reads = 1091304 mem = 536877489 peak = 1040826368
[M::mem_process_seqs] Processed 698482 reads in 187.893 CPU sec, 5.930 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290849, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.02, 119.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 231, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (231.67, 76.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RR
[M::process] read 698672 sequences (100000254 bp) in 2.300 CPU sec, 2.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290707, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.06, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698410 reads in 151.045 CPU sec, 4.778 real sec
[M::mem_process_seqs] Processed 698168 reads in 87.122 CPU sec, 2.751 real sec
sorting block 77, #reads = 1090827 mem = 536879341 peak = 1040826368
[M::process] read 701194 sequences (100000140 bp) in 1.981 CPU sec, 2.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290846, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.17, 119.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1090577 mem = 536883715 peak = 1059696640
[M::process] read 708304 sequences (100000113 bp) in 2.143 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291241, 12, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 225, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 433)
[M::mem_pestat] mean and std.dev: (219.20, 75.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (241.02, 116.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 307, 872)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138)
[M::mem_pestat] mean and std.dev: (325.60, 204.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2771)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 705924 sequences (100000185 bp) in 1.440 CPU sec, 1.437 real sec...
[M::mem_process_seqs] Processed 698672 reads in 147.218 CPU sec, 4.680 real sec
[M::mem_process_seqs] Processed 701194 reads in 99.114 CPU sec, 3.135 real sec
sorting block 79, #reads = 1086905 mem = 536879121 peak = 1059696640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 294275, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 202, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.45, 105.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705216 sequences (100000128 bp) in 2.588 CPU sec, 2.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293569, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.24, 109.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 708304 reads in 141.692 CPU sec, 4.507 real sec
[M::mem_process_seqs] Processed 705924 reads in 90.577 CPU sec, 2.856 real sec
[M::process] read 706604 sequences (100000254 bp) in 2.203 CPU sec, 2.258 real sec...
sorting block 80, #reads = 1089176 mem = 536882165 peak = 1059696640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293000, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (233.18, 111.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 222, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161)
[M::mem_pestat] mean and std.dev: (302.42, 203.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1478)
[M::mem_pestat] skip orientation RR
[M::process] read 704672 sequences (100000092 bp) in 2.151 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293909, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.49, 109.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704310 sequences (100000029 bp) in 1.370 CPU sec, 1.373 real sec...
[M::mem_process_seqs] Processed 705216 reads in 148.664 CPU sec, 4.735 real sec
[M::mem_process_seqs] Processed 706604 reads in 97.230 CPU sec, 3.082 real sec
sorting block 81, #reads = 1093637 mem = 536879982 peak = 1059696640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293424, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.59, 113.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703270 sequences (100000143 bp) in 2.180 CPU sec, 2.291 real sec...
[M::process] read 701850 sequences (100000009 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292887, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (234.13, 112.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704672 reads in 146.341 CPU sec, 4.661 real sec
sorting block 82, #reads = 1092358 mem = 536876984 peak = 1060413440
[M::mem_process_seqs] Processed 704310 reads in 91.917 CPU sec, 2.922 real sec
sorting block 83, #reads = 1093850 mem = 536879239 peak = 1152335872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292326, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 239, 708)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1770)
[M::mem_pestat] mean and std.dev: (419.00, 340.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2301)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.93, 116.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699924 sequences (100000038 bp) in 2.156 CPU sec, 2.244 real sec...
[M::process] read 699312 sequences (100000269 bp) in 1.390 CPU sec, 1.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291805, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (244.30, 119.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703270 reads in 144.469 CPU sec, 4.595 real sec
[M::mem_process_seqs] Processed 701850 reads in 90.621 CPU sec, 2.882 real sec
sorting block 84, #reads = 1092278 mem = 536877422 peak = 1152335872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291626, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.72, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 225, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (53, 393)
[M::mem_pestat] mean and std.dev: (213.22, 78.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 526)
[M::mem_pestat] skip orientation RR
[M::process] read 699448 sequences (100000146 bp) in 2.298 CPU sec, 2.445 real sec...
[M::process] read 698686 sequences (100000263 bp) in 1.350 CPU sec, 1.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291115, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 203, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321)
[M::mem_pestat] mean and std.dev: (319.92, 289.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1704)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.43, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699924 reads in 144.022 CPU sec, 4.582 real sec
[M::mem_process_seqs] Processed 699312 reads in 90.085 CPU sec, 2.858 real sec
sorting block 85, #reads = 1091718 mem = 536879898 peak = 1152335872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291413, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.57, 119.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 229, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 496)
[M::mem_pestat] mean and std.dev: (236.82, 92.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] skip orientation RR
[M::process] read 698100 sequences (100000112 bp) in 2.253 CPU sec, 2.342 real sec...
[M::process] read 697520 sequences (100000179 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290713, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 193, 1024)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756)
[M::mem_pestat] mean and std.dev: (289.44, 269.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3622)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.24, 120.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699448 reads in 141.182 CPU sec, 4.487 real sec
sorting block 86, #reads = 1091423 mem = 536885384 peak = 1152335872
[M::mem_process_seqs] Processed 698686 reads in 88.070 CPU sec, 2.797 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290476, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.74, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 87, #reads = 1092096 mem = 536880307 peak = 1152335872
[M::process] read 697252 sequences (100000115 bp) in 2.071 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290792, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.14, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697612 sequences (100000091 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_process_seqs] Processed 698100 reads in 140.574 CPU sec, 4.476 real sec
[M::mem_process_seqs] Processed 697520 reads in 87.977 CPU sec, 2.790 real sec
[M::process] read 49626 sequences (7099284 bp) in 0.579 CPU sec, 0.584 real sec...
sorting block 88, #reads = 1091556 mem = 536878844 peak = 1152335872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290741, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.96, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 469, 714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1732)
[M::mem_pestat] mean and std.dev: (454.58, 298.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2241)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290390, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.14, 120.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 20607, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (244.93, 115.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697252 reads in 142.195 CPU sec, 4.608 real sec
[M::mem_process_seqs] Processed 697612 reads in 92.369 CPU sec, 2.989 real sec
[M::mem_process_seqs] Processed 49626 reads in 93.454 CPU sec, 3.029 real sec
sorting block 89, #reads = 1091567 mem = 536886805 peak = 1152335872
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 98695580
overall: 70061875200 mem 11501.786 user 397.396 sys 877.695 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 877.729 sec; CPU: 11899.185 sec
sorting block 90, #reads = 802526 mem = 395255673 peak = 1192366080
uniq header count 1
merging 91 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5213445391
output reads: 99199372
bam_mem_sort: 91 calls 34.859 user 0.492 sys 35.551 real
bam_write: 91 calls 32.619 user 0.934 sys 33.849 real
execute: 1 calls 19.770 user 1.439 sys 905.843 real
merge_files: 1 calls 19.757 user 1.414 sys 27.100 real
parse_chunk: 1452 calls 48.791 user 13.693 sys 63.490 real
read_chunk: 3922 calls 42.170 user 36.268 sys 347.722 real
sort_block: 1 calls 33.334 user 35.696 sys 334.865 real
write_chunk: 2468 calls 19.188 user 0.874 sys 25.757 real
overall: 3018338304 mem 1059.322 user 60.655 sys 905.865 real
ls: cannot access '*.cram': No such file or directory