cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1676.042 CPU sec, 1375.582 real sec
[M::process] read 683682 sequences (100000120 bp) in 0.940 CPU sec, 0.944 real sec...
[M::process] read 682030 sequences (100000246 bp) in 1.240 CPU sec, 1.245 real sec...
[M::process] read 681364 sequences (100000220 bp) in 1.190 CPU sec, 1.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278733, 8, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 300, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (287.05, 101.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (350.39, 168.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 253, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (262.75, 108.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680736 sequences (100000239 bp) in 1.119 CPU sec, 1.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278571, 8, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 271, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (266.33, 80.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 346, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (360.41, 171.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 249, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (275.71, 184.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277800, 5, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 308, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (319.51, 197.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1377)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 351, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (363.60, 171.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 296, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (280.93, 140.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683682 reads in 247.643 CPU sec, 7.941 real sec
[M::mem_process_seqs] Processed 682030 reads in 165.391 CPU sec, 5.383 real sec
[M::mem_process_seqs] Processed 681364 reads in 180.898 CPU sec, 5.891 real sec
sorting block 0, #reads = 1069474 mem = 536880092 peak = 599355392
[M::process] read 682182 sequences (100000096 bp) in 1.756 CPU sec, 1.914 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 277981, 0, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 228, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (266.88, 146.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 358, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (370.03, 174.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 287, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265)
[M::mem_pestat] mean and std.dev: (383.88, 261.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1607)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681570 sequences (100000101 bp) in 1.304 CPU sec, 1.328 real sec...
[M::process] read 681272 sequences (100000071 bp) in 1.100 CPU sec, 1.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277874, 6, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 323, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (280.71, 105.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 348, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (362.42, 173.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (275.47, 116.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680736 reads in 155.691 CPU sec, 4.960 real sec
sorting block 1, #reads = 1069193 mem = 536887932 peak = 803827712
[M::mem_process_seqs] Processed 682182 reads in 99.524 CPU sec, 3.170 real sec
sorting block 2, #reads = 1070101 mem = 536877781 peak = 918138880
[M::process] read 681994 sequences (100000134 bp) in 1.524 CPU sec, 1.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277648, 5, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 236, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (255.48, 105.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 349, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (363.37, 172.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 258, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (305.62, 181.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1622)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681902 sequences (100000246 bp) in 1.270 CPU sec, 1.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277672, 14, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 210, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (252.81, 137.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (361.51, 171.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1117, 2827, 8133)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22165)
[M::mem_pestat] mean and std.dev: (3443.50, 3089.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 29181)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 331, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (344.64, 145.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277775, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 261, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (274.49, 137.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (352.05, 165.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 344, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (323.68, 174.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681570 reads in 226.561 CPU sec, 7.259 real sec
[M::mem_process_seqs] Processed 681272 reads in 169.124 CPU sec, 5.446 real sec
[M::mem_process_seqs] Processed 681994 reads in 184.858 CPU sec, 5.967 real sec
sorting block 3, #reads = 1071093 mem = 536881282 peak = 918138880
[M::process] read 681284 sequences (100000212 bp) in 1.862 CPU sec, 2.016 real sec...
sorting block 4, #reads = 1069787 mem = 536879811 peak = 918138880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277017, 8, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 282, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (308.38, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (351.73, 165.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 294, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (292.70, 110.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680768 sequences (100000155 bp) in 1.575 CPU sec, 1.649 real sec...
[M::process] read 680208 sequences (100000203 bp) in 1.210 CPU sec, 1.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277175, 2, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113)
[M::mem_pestat] mean and std.dev: (302.76, 166.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1426)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 340, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (355.91, 168.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 258, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (275.61, 135.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681902 reads in 163.979 CPU sec, 5.225 real sec
[M::mem_process_seqs] Processed 681284 reads in 103.082 CPU sec, 3.281 real sec
sorting block 5, #reads = 1066804 mem = 536885077 peak = 918138880
[M::process] read 682172 sequences (100000301 bp) in 1.839 CPU sec, 1.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 277452, 5, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 264, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (314.64, 161.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 342, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (357.88, 168.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 275, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224)
[M::mem_pestat] mean and std.dev: (297.46, 157.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1566)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682208 sequences (100000074 bp) in 1.248 CPU sec, 1.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278414, 21, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 201, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (222.40, 94.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 691)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (355.43, 169.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (501, 717, 2474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6420)
[M::mem_pestat] mean and std.dev: (1321.86, 979.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8393)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 301, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (300.12, 112.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277210, 13, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 375, 620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1464)
[M::mem_pestat] mean and std.dev: (386.74, 332.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1886)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 344, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (359.50, 168.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (843, 843, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (843, 843)
[M::mem_pestat] mean and std.dev: (843.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (843, 843)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 258, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (254.95, 114.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680768 reads in 222.227 CPU sec, 7.124 real sec
[M::mem_process_seqs] Processed 682172 reads in 164.457 CPU sec, 5.290 real sec
sorting block 6, #reads = 1068760 mem = 536882578 peak = 918138880
[M::mem_process_seqs] Processed 680208 reads in 179.498 CPU sec, 5.793 real sec
[M::process] read 681348 sequences (100000271 bp) in 2.058 CPU sec, 2.262 real sec...
sorting block 7, #reads = 1070571 mem = 536878102 peak = 918138880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278018, 3, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 270, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (253.53, 158.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 348, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (362.29, 174.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 392, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215)
[M::mem_pestat] mean and std.dev: (337.28, 167.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1572)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680630 sequences (100000237 bp) in 1.433 CPU sec, 1.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278107, 2, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (258, 359, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137)
[M::mem_pestat] mean and std.dev: (275.50, 115.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1430)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 355, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (368.65, 176.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 345, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (321.45, 124.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681242 sequences (100000017 bp) in 1.270 CPU sec, 1.271 real sec...
[M::mem_process_seqs] Processed 682208 reads in 156.491 CPU sec, 5.007 real sec
[M::mem_process_seqs] Processed 681348 reads in 100.669 CPU sec, 3.198 real sec
sorting block 8, #reads = 1070859 mem = 536877899 peak = 918138880
[M::process] read 681512 sequences (100000250 bp) in 1.780 CPU sec, 1.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277630, 7, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 264, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (280.02, 144.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 362, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (373.59, 176.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 259, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (306.18, 165.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681254 sequences (100000287 bp) in 1.320 CPU sec, 1.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278034, 10, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194)
[M::mem_pestat] mean and std.dev: (364.34, 253.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1525)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 357, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (370.10, 176.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (730, 4136, 4136)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10948)
[M::mem_pestat] mean and std.dev: (2903.50, 2422.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14354)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114)
[M::mem_pestat] mean and std.dev: (299.62, 128.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1417)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680630 reads in 155.255 CPU sec, 4.968 real sec
sorting block 9, #reads = 1071410 mem = 536880337 peak = 918138880
[M::mem_process_seqs] Processed 681242 reads in 99.221 CPU sec, 3.179 real sec
[M::process] read 681590 sequences (100000205 bp) in 1.836 CPU sec, 1.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278125, 5, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 341, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281)
[M::mem_pestat] mean and std.dev: (284.59, 141.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1642)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 350, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (365.40, 174.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 197, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (219.39, 64.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 585)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682422 sequences (100000258 bp) in 1.338 CPU sec, 1.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277989, 3, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 305, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (310.88, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 348, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (363.22, 173.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 271, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (58, 488)
[M::mem_pestat] mean and std.dev: (275.68, 90.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277975, 7, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 272, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (269.55, 161.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (359.38, 171.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 227, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556)
[M::mem_pestat] mean and std.dev: (217.53, 91.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681512 reads in 224.677 CPU sec, 7.216 real sec
sorting block 10, #reads = 1070888 mem = 536878025 peak = 918138880
[M::mem_process_seqs] Processed 681254 reads in 167.448 CPU sec, 5.400 real sec
[M::mem_process_seqs] Processed 681590 reads in 183.177 CPU sec, 5.934 real sec
sorting block 11, #reads = 1070939 mem = 536878987 peak = 918138880
[M::process] read 681586 sequences (100000182 bp) in 2.123 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278375, 4, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 250, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (261.02, 114.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (352.88, 169.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 259, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (258.78, 162.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681046 sequences (100000199 bp) in 1.567 CPU sec, 1.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277718, 8, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 355, 689)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1721)
[M::mem_pestat] mean and std.dev: (393.44, 325.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2237)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (358.87, 171.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 242, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (293.27, 139.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680638 sequences (100000033 bp) in 1.300 CPU sec, 1.300 real sec...
[M::mem_process_seqs] Processed 682422 reads in 162.608 CPU sec, 5.204 real sec
sorting block 12, #reads = 1070411 mem = 536878361 peak = 918138880
[M::mem_process_seqs] Processed 681586 reads in 102.799 CPU sec, 3.264 real sec
sorting block 13, #reads = 1067864 mem = 536882596 peak = 918138880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277426, 3, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 214, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (244.77, 109.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (359.10, 170.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 281, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (311.73, 135.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680794 sequences (100000193 bp) in 1.892 CPU sec, 2.043 real sec...
[M::process] read 682568 sequences (100000048 bp) in 1.360 CPU sec, 1.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 277739, 5, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 186, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (202.09, 101.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 344, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (360.28, 170.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 284, 642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516)
[M::mem_pestat] mean and std.dev: (421.28, 335.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1953)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681046 reads in 155.644 CPU sec, 4.984 real sec
[M::mem_process_seqs] Processed 680638 reads in 99.521 CPU sec, 3.176 real sec
sorting block 14, #reads = 1069520 mem = 536877543 peak = 918138880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277797, 16, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 244, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223)
[M::mem_pestat] mean and std.dev: (309.32, 206.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1566)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 348, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (364.02, 173.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (406, 569, 1743)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4417)
[M::mem_pestat] mean and std.dev: (959.20, 890.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5754)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 397, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (323.49, 130.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681352 sequences (100000186 bp) in 1.918 CPU sec, 2.078 real sec...
[M::process] read 680484 sequences (100000288 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278713, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 245, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (229.58, 96.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 342, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (358.49, 173.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 326, 647)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1459)
[M::mem_pestat] mean and std.dev: (403.26, 296.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1865)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680794 reads in 153.073 CPU sec, 4.901 real sec
[M::mem_process_seqs] Processed 682568 reads in 98.268 CPU sec, 3.140 real sec
sorting block 15, #reads = 1069112 mem = 536878690 peak = 918138880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278682, 4, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 238, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (248.94, 88.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (367.00, 175.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 270, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 542)
[M::mem_pestat] mean and std.dev: (262.30, 91.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 650)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 679730 sequences (100000035 bp) in 2.005 CPU sec, 2.147 real sec...
[M::process] read 681548 sequences (100000139 bp) in 1.350 CPU sec, 1.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 278031, 7, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 259, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (283.02, 146.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 360, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (372.82, 176.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (309, 441, 685)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437)
[M::mem_pestat] mean and std.dev: (465.57, 199.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1813)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681352 reads in 150.985 CPU sec, 4.827 real sec
sorting block 16, #reads = 1070405 mem = 536877326 peak = 918138880
[M::mem_process_seqs] Processed 680484 reads in 97.290 CPU sec, 3.108 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 278004, 5, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 241, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (272.17, 130.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 365, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (378.04, 178.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 378, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (304.29, 134.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680890 sequences (100000279 bp) in 2.090 CPU sec, 2.242 real sec...
[M::process] read 681232 sequences (100000094 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277805, 8, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 303, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208)
[M::mem_pestat] mean and std.dev: (321.91, 157.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1544)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 351, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (366.08, 176.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 382, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240)
[M::mem_pestat] mean and std.dev: (372.39, 161.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1571)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 679730 reads in 153.150 CPU sec, 4.890 real sec
sorting block 17, #reads = 1070120 mem = 536877010 peak = 921673728
[M::mem_process_seqs] Processed 681548 reads in 98.448 CPU sec, 3.146 real sec
sorting block 18, #reads = 1070421 mem = 536886506 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277633, 1, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 280, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211)
[M::mem_pestat] mean and std.dev: (305.36, 266.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1559)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 351, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (366.66, 175.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 275, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (309.27, 130.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681632 sequences (100000174 bp) in 2.082 CPU sec, 2.258 real sec...
[M::process] read 681512 sequences (100000009 bp) in 1.370 CPU sec, 1.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 277884, 7, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 257, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (257.13, 129.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (363.55, 174.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 362, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (338.87, 145.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680890 reads in 154.196 CPU sec, 4.918 real sec
[M::mem_process_seqs] Processed 681232 reads in 98.277 CPU sec, 3.140 real sec
sorting block 19, #reads = 1069813 mem = 536879178 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277956, 9, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 274, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (274.47, 130.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (359.48, 172.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 272, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (293.49, 103.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681152 sequences (100000201 bp) in 2.088 CPU sec, 2.287 real sec...
[M::process] read 680532 sequences (100000267 bp) in 1.310 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 277893, 3, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 260, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (277.42, 130.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (361.22, 173.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (386.89, 219.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1606)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681632 reads in 152.268 CPU sec, 4.861 real sec
[M::mem_process_seqs] Processed 681512 reads in 98.249 CPU sec, 3.134 real sec
sorting block 20, #reads = 1069648 mem = 536883630 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278563, 3, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 340, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1384)
[M::mem_pestat] mean and std.dev: (393.18, 265.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1778)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (362.61, 174.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 288, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (254.43, 120.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680730 sequences (100000182 bp) in 2.274 CPU sec, 2.450 real sec...
[M::process] read 681446 sequences (100000286 bp) in 1.330 CPU sec, 1.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277734, 15, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 253, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198)
[M::mem_pestat] mean and std.dev: (265.44, 185.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1540)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 347, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (364.18, 174.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1030, 1185, 2058)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4114)
[M::mem_pestat] mean and std.dev: (1338.13, 492.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5142)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 293, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (308.94, 134.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681152 reads in 147.903 CPU sec, 4.704 real sec
sorting block 21, #reads = 1069380 mem = 536881932 peak = 988495872
[M::mem_process_seqs] Processed 680532 reads in 95.249 CPU sec, 3.031 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277900, 5, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 265, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.67, 111.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (366.06, 175.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 240, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (255.36, 110.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1071075 mem = 536884599 peak = 988495872
[M::process] read 681238 sequences (100000207 bp) in 1.943 CPU sec, 2.088 real sec...
[M::process] read 680656 sequences (100000057 bp) in 1.310 CPU sec, 1.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 278204, 1, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 262, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319)
[M::mem_pestat] mean and std.dev: (299.60, 219.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1701)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (361.62, 173.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 364, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (312.46, 114.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680730 reads in 147.688 CPU sec, 4.710 real sec
[M::mem_process_seqs] Processed 681446 reads in 96.786 CPU sec, 3.074 real sec
sorting block 23, #reads = 1068908 mem = 536881308 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277587, 8, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 366, 2195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6231)
[M::mem_pestat] mean and std.dev: (1119.14, 1660.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8249)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 352, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (366.77, 176.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 346, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (364.67, 189.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1616)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 679886 sequences (100000171 bp) in 2.073 CPU sec, 2.221 real sec...
[M::process] read 679858 sequences (100000135 bp) in 1.300 CPU sec, 1.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278186, 6, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (309, 389, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (315.79, 98.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 358, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (373.33, 179.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 315, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (301.35, 204.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681238 reads in 147.794 CPU sec, 4.698 real sec
[M::mem_process_seqs] Processed 680656 reads in 95.256 CPU sec, 3.033 real sec
sorting block 24, #reads = 1070837 mem = 536881135 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277696, 10, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 379, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (347.28, 175.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1361)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 364, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (376.91, 178.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1153, 1549, 1549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (361, 2341)
[M::mem_pestat] mean and std.dev: (1141.50, 401.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2748)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 262, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (264.06, 139.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681244 sequences (100000184 bp) in 1.950 CPU sec, 2.077 real sec...
[M::process] read 681050 sequences (100000012 bp) in 1.320 CPU sec, 1.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277408, 5, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 294, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (325.16, 162.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 366, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (378.84, 179.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 278, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469)
[M::mem_pestat] mean and std.dev: (353.64, 231.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1903)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 679886 reads in 147.040 CPU sec, 4.690 real sec
[M::mem_process_seqs] Processed 679858 reads in 95.260 CPU sec, 3.025 real sec
sorting block 25, #reads = 1072063 mem = 536879941 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278261, 8, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 297, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 543)
[M::mem_pestat] mean and std.dev: (283.66, 82.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 646)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 352, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (368.50, 177.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 298, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (293.77, 94.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681304 sequences (100000278 bp) in 2.232 CPU sec, 2.352 real sec...
[M::process] read 681168 sequences (100000049 bp) in 1.310 CPU sec, 1.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 277283, 9, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 357, 644)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1598)
[M::mem_pestat] mean and std.dev: (434.00, 366.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2075)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 349, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (365.37, 174.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 260, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148)
[M::mem_pestat] mean and std.dev: (278.70, 191.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1475)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681244 reads in 151.718 CPU sec, 4.825 real sec
sorting block 26, #reads = 1071885 mem = 536878525 peak = 988495872
[M::mem_process_seqs] Processed 681050 reads in 98.169 CPU sec, 3.125 real sec
sorting block 27, #reads = 1070882 mem = 536880763 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277740, 5, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 198, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (198.44, 96.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (362.03, 173.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 287, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (264.51, 135.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680900 sequences (100000241 bp) in 2.273 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277625, 9, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 295, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (312.14, 179.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (361.45, 171.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 215, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512)
[M::mem_pestat] mean and std.dev: (237.00, 81.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 623)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680908 sequences (100000018 bp) in 1.570 CPU sec, 1.564 real sec...
[M::mem_process_seqs] Processed 681304 reads in 152.114 CPU sec, 4.838 real sec
[M::mem_process_seqs] Processed 681168 reads in 98.105 CPU sec, 3.108 real sec
sorting block 28, #reads = 1070875 mem = 536881622 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277331, 7, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 234, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (274.97, 138.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (362.77, 173.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 313, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (287.14, 161.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680618 sequences (100000146 bp) in 2.866 CPU sec, 2.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277980, 5, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 238, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (230.12, 96.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (362.49, 173.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 352, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (356.52, 158.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680900 reads in 152.372 CPU sec, 4.833 real sec
[M::process] read 679980 sequences (100000119 bp) in 1.580 CPU sec, 1.580 real sec...
[M::mem_process_seqs] Processed 680908 reads in 98.378 CPU sec, 3.112 real sec
sorting block 29, #reads = 1070494 mem = 536880301 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277814, 7, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 196, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (231.23, 133.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 349, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (364.61, 172.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 279, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (256.93, 80.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684910 sequences (100000175 bp) in 2.628 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277738, 6, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 292, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (316.35, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (368.20, 174.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 297, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (303.02, 132.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680618 reads in 148.250 CPU sec, 4.729 real sec
[M::mem_process_seqs] Processed 679980 reads in 97.408 CPU sec, 3.072 real sec
[M::process] read 685798 sequences (100000081 bp) in 1.926 CPU sec, 1.974 real sec...
sorting block 30, #reads = 1070010 mem = 536880004 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279247, 5, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 249, 746)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1946)
[M::mem_pestat] mean and std.dev: (348.14, 276.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2546)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 330, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (348.76, 173.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 319, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (325.51, 161.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 31, #reads = 1070198 mem = 536878892 peak = 988495872
[M::process] read 684288 sequences (100000152 bp) in 2.744 CPU sec, 2.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280030, 9, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 354, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (311.03, 160.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (345.41, 171.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 353, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (341.63, 124.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684910 reads in 158.665 CPU sec, 5.042 real sec
[M::process] read 683788 sequences (100000131 bp) in 1.677 CPU sec, 1.678 real sec...
[M::mem_process_seqs] Processed 685798 reads in 103.693 CPU sec, 3.278 real sec
sorting block 32, #reads = 1069258 mem = 536878720 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279556, 5, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 236, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (241.79, 93.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 339, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (353.12, 170.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (246.49, 99.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686176 sequences (100000064 bp) in 2.548 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 279047, 5, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 239, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (313.36, 199.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1413)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 339, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (354.00, 170.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 241, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (255.57, 128.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685638 sequences (100000240 bp) in 1.540 CPU sec, 1.545 real sec...
[M::mem_process_seqs] Processed 684288 reads in 158.554 CPU sec, 5.043 real sec
[M::mem_process_seqs] Processed 683788 reads in 101.984 CPU sec, 3.227 real sec
sorting block 33, #reads = 1068224 mem = 536879605 peak = 988495872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280246, 4, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 252, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (272.15, 120.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (340.65, 167.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 312, 666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1628)
[M::mem_pestat] mean and std.dev: (363.31, 241.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2109)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684004 sequences (100000183 bp) in 2.447 CPU sec, 2.576 real sec...
[M::process] read 686250 sequences (100000095 bp) in 1.550 CPU sec, 1.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279939, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 393, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (335.07, 151.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (341.59, 166.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (300.15, 193.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1401)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686176 reads in 165.869 CPU sec, 5.271 real sec
[M::mem_process_seqs] Processed 685638 reads in 108.197 CPU sec, 3.442 real sec
sorting block 34, #reads = 1069661 mem = 536879163 peak = 991318016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279120, 1, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 231, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (288.12, 222.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 334, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (350.07, 168.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 280, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329)
[M::mem_pestat] mean and std.dev: (340.71, 183.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1701)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683602 sequences (100000016 bp) in 2.356 CPU sec, 2.503 real sec...
[M::process] read 683624 sequences (100000246 bp) in 1.520 CPU sec, 1.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279908, 8, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 248, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (280.47, 139.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 318, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (335.09, 161.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 298, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (320.87, 171.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684004 reads in 171.573 CPU sec, 5.464 real sec
sorting block 35, #reads = 1065927 mem = 536879041 peak = 991318016
[M::mem_process_seqs] Processed 686250 reads in 117.226 CPU sec, 3.728 real sec
sorting block 36, #reads = 1062731 mem = 536882517 peak = 1033932800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278948, 7, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 266, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (312.39, 191.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (347.84, 166.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 383, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (338.58, 127.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684862 sequences (100000240 bp) in 2.690 CPU sec, 2.834 real sec...
[M::process] read 689050 sequences (100000031 bp) in 1.550 CPU sec, 1.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279003, 5, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 392, 917)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369)
[M::mem_pestat] mean and std.dev: (514.59, 462.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3095)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 336, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (351.57, 169.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 296, 561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1275)
[M::mem_pestat] mean and std.dev: (358.11, 213.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1632)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683602 reads in 172.500 CPU sec, 5.475 real sec
[M::mem_process_seqs] Processed 683624 reads in 111.688 CPU sec, 3.551 real sec
sorting block 37, #reads = 1063246 mem = 536878141 peak = 1033932800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 279525, 7, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 387, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (330.94, 137.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (345.01, 170.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 306, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309)
[M::mem_pestat] mean and std.dev: (388.82, 290.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1680)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686092 sequences (100000018 bp) in 2.258 CPU sec, 2.387 real sec...
[M::process] read 684480 sequences (100000251 bp) in 1.630 CPU sec, 1.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281412, 7, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 292, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108)
[M::mem_pestat] mean and std.dev: (306.62, 157.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1414)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 307, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (327.32, 163.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 262, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (313.50, 168.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684862 reads in 173.695 CPU sec, 5.533 real sec
[M::mem_process_seqs] Processed 689050 reads in 113.349 CPU sec, 3.600 real sec
sorting block 38, #reads = 1061420 mem = 536877853 peak = 1033932800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280550, 4, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 311, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (261.59, 105.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 321, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (338.87, 165.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 292, 657)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1591)
[M::mem_pestat] mean and std.dev: (403.39, 284.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2058)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685280 sequences (100000008 bp) in 3.002 CPU sec, 3.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280077, 2, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 270, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (264.61, 99.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 332, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (348.31, 168.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 257, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (278.25, 138.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685060 sequences (100000076 bp) in 1.630 CPU sec, 1.628 real sec...
[M::mem_process_seqs] Processed 686092 reads in 167.605 CPU sec, 5.324 real sec
sorting block 39, #reads = 1062302 mem = 536879620 peak = 1033932800
[M::mem_process_seqs] Processed 684480 reads in 106.862 CPU sec, 3.380 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280387, 5, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 222, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (237.58, 110.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 325, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (343.14, 167.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 249, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (277.41, 114.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 40, #reads = 1064922 mem = 536877775 peak = 1033932800
[M::process] read 685038 sequences (100000204 bp) in 2.431 CPU sec, 2.557 real sec...
[M::process] read 684876 sequences (100000020 bp) in 1.380 CPU sec, 1.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279887, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 240, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094)
[M::mem_pestat] mean and std.dev: (313.59, 223.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1393)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (344.08, 168.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 331, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (349.81, 178.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685280 reads in 162.012 CPU sec, 5.163 real sec
[M::mem_process_seqs] Processed 685060 reads in 105.162 CPU sec, 3.342 real sec
sorting block 41, #reads = 1068908 mem = 536881258 peak = 1033932800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 279794, 11, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 304, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (294.56, 141.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (344.99, 168.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1199, 1199, 1779)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (39, 2939)
[M::mem_pestat] mean and std.dev: (1521.22, 288.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3519)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 245, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (239.93, 68.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682282 sequences (100000085 bp) in 2.261 CPU sec, 2.401 real sec...
[M::process] read 681854 sequences (100000294 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279065, 8, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 221, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (241.50, 114.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (346.65, 170.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 303, 754)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792)
[M::mem_pestat] mean and std.dev: (348.37, 207.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2311)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685038 reads in 170.652 CPU sec, 5.431 real sec
[M::mem_process_seqs] Processed 684876 reads in 113.075 CPU sec, 3.602 real sec
sorting block 42, #reads = 1065751 mem = 536879552 peak = 1033932800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279256, 5, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 199, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (226.32, 99.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 343, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (361.46, 176.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 318, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (307.29, 154.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681396 sequences (100000055 bp) in 2.585 CPU sec, 2.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279097, 6, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 293, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112)
[M::mem_pestat] mean and std.dev: (391.14, 313.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2760)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 351, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (369.22, 180.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 385, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318)
[M::mem_pestat] mean and std.dev: (389.10, 209.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1679)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685172 sequences (100000153 bp) in 1.590 CPU sec, 1.595 real sec...
[M::mem_process_seqs] Processed 682282 reads in 160.602 CPU sec, 5.105 real sec
[M::mem_process_seqs] Processed 681854 reads in 104.015 CPU sec, 3.292 real sec
sorting block 43, #reads = 1063121 mem = 536880159 peak = 1033932800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278269, 6, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 221, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (231.81, 105.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 352, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (370.67, 182.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 253, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (288.90, 144.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683802 sequences (100000297 bp) in 2.802 CPU sec, 2.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279309, 20, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 327, 5374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15754)
[M::mem_pestat] mean and std.dev: (2243.86, 3598.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20944)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 329, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (352.29, 178.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 1063, 2021)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5667)
[M::mem_pestat] mean and std.dev: (820.33, 866.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7490)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 347, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117)
[M::mem_pestat] mean and std.dev: (351.00, 169.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1407)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682126 sequences (100000250 bp) in 1.560 CPU sec, 1.558 real sec...
[M::mem_process_seqs] Processed 681396 reads in 165.622 CPU sec, 5.266 real sec
sorting block 44, #reads = 1067620 mem = 536883135 peak = 1033932800
[M::mem_process_seqs] Processed 685172 reads in 108.082 CPU sec, 3.427 real sec
sorting block 45, #reads = 1063126 mem = 536881337 peak = 1061273600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280236, 2, 72)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 319, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (315.57, 114.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 342, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (360.35, 179.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 267, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (318.76, 163.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681938 sequences (100000207 bp) in 2.424 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279282, 9, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 337, 673)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1641)
[M::mem_pestat] mean and std.dev: (386.88, 269.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2125)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 349, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (364.91, 177.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 347, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (354.36, 154.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684362 sequences (100000186 bp) in 1.580 CPU sec, 1.581 real sec...
[M::mem_process_seqs] Processed 683802 reads in 151.568 CPU sec, 4.818 real sec
[M::mem_process_seqs] Processed 682126 reads in 97.046 CPU sec, 3.070 real sec
sorting block 46, #reads = 1070661 mem = 536881719 peak = 1061273600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278757, 6, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 289, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (317.27, 187.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 355, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (370.04, 180.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 262, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (261.50, 109.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683468 sequences (100000274 bp) in 2.855 CPU sec, 2.995 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279673, 3, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 319, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353)
[M::mem_pestat] mean and std.dev: (397.95, 322.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1746)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 333, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (353.69, 176.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 299, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (275.32, 125.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681938 reads in 151.581 CPU sec, 4.815 real sec
[M::process] read 682158 sequences (100000125 bp) in 1.658 CPU sec, 1.670 real sec...
[M::mem_process_seqs] Processed 684362 reads in 99.114 CPU sec, 3.129 real sec
sorting block 47, #reads = 1070274 mem = 536881340 peak = 1061273600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 279835, 8, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198)
[M::mem_pestat] mean and std.dev: (327.14, 189.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1540)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 343, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (361.33, 178.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 302, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254)
[M::mem_pestat] mean and std.dev: (312.23, 153.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1609)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682708 sequences (100000262 bp) in 2.530 CPU sec, 2.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278971, 13, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 184, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (213.96, 97.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 672)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 349, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (367.64, 181.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (498, 1549, 5110)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14334)
[M::mem_pestat] mean and std.dev: (2128.38, 2032.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18946)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 304, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (292.52, 119.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682598 sequences (100000273 bp) in 1.570 CPU sec, 1.573 real sec...
[M::mem_process_seqs] Processed 683468 reads in 155.991 CPU sec, 4.963 real sec
sorting block 48, #reads = 1071084 mem = 536882103 peak = 1061273600
[M::mem_process_seqs] Processed 682158 reads in 101.735 CPU sec, 3.221 real sec
sorting block 49, #reads = 1068141 mem = 536877745 peak = 1061273600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278286, 3, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 260, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (251.69, 100.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 343, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (362.65, 179.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 314, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (320.67, 130.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682492 sequences (100000064 bp) in 2.694 CPU sec, 2.807 real sec...
[M::process] read 682634 sequences (100000254 bp) in 1.510 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278929, 6, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 260, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (282.78, 146.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 343, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (363.45, 180.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (334.27, 175.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682708 reads in 161.929 CPU sec, 5.144 real sec
[M::mem_process_seqs] Processed 682598 reads in 105.146 CPU sec, 3.333 real sec
sorting block 50, #reads = 1066135 mem = 536878976 peak = 1061273600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278655, 2, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 220, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (246.50, 131.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 343, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (364.72, 182.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 356, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199)
[M::mem_pestat] mean and std.dev: (376.28, 212.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1521)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685056 sequences (100000021 bp) in 2.297 CPU sec, 2.416 real sec...
[M::process] read 685952 sequences (100000072 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279485, 6, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 318, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (308.68, 112.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 340, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (364.15, 183.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 326, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (302.36, 150.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682492 reads in 160.778 CPU sec, 5.120 real sec
[M::mem_process_seqs] Processed 682634 reads in 104.037 CPU sec, 3.308 real sec
sorting block 51, #reads = 1066376 mem = 536877054 peak = 1061273600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279388, 6, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 223, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.92, 128.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (351.12, 179.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 262, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (282.87, 125.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683556 sequences (100000292 bp) in 2.376 CPU sec, 2.515 real sec...
[M::process] read 682230 sequences (100000063 bp) in 1.350 CPU sec, 1.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 280278, 14, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 207, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (184.67, 67.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 645)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 326, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (348.39, 176.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (55, 588, 2306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6808)
[M::mem_pestat] mean and std.dev: (1192.43, 1368.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9059)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 258, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (299.12, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685056 reads in 169.726 CPU sec, 5.404 real sec
sorting block 52, #reads = 1064172 mem = 536878824 peak = 1061273600
[M::mem_process_seqs] Processed 685952 reads in 107.252 CPU sec, 3.414 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279526, 2, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 308, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (255.54, 105.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 341, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (358.59, 175.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (293, 407, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103)
[M::mem_pestat] mean and std.dev: (399.24, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1373)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683244 sequences (100000190 bp) in 2.038 CPU sec, 2.156 real sec...
[M::process] read 682348 sequences (100000226 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279258, 5, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (271, 396, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (333.40, 129.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 351, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (366.03, 176.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 277, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (272.62, 137.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683556 reads in 158.472 CPU sec, 5.049 real sec
sorting block 53, #reads = 1064417 mem = 536879837 peak = 1061273600
[M::mem_process_seqs] Processed 682230 reads in 102.340 CPU sec, 3.260 real sec
sorting block 54, #reads = 1066560 mem = 536879110 peak = 1114193920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279079, 10, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 336, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (333.72, 178.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 340, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (357.32, 174.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (336, 1264, 1868)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4932)
[M::mem_pestat] mean and std.dev: (1310.00, 954.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6464)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 288, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (316.65, 155.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682110 sequences (100000109 bp) in 2.396 CPU sec, 2.545 real sec...
[M::process] read 681788 sequences (100000258 bp) in 1.350 CPU sec, 1.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278980, 5, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 321, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (320.03, 164.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 344, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (360.72, 173.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 298, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (261.20, 143.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683244 reads in 159.352 CPU sec, 5.069 real sec
[M::mem_process_seqs] Processed 682348 reads in 103.520 CPU sec, 3.296 real sec
sorting block 55, #reads = 1067189 mem = 536881992 peak = 1114193920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278345, 6, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 228, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1500)
[M::mem_pestat] mean and std.dev: (393.72, 366.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1945)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (354.70, 168.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 243, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (276.98, 155.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681548 sequences (100000295 bp) in 2.034 CPU sec, 2.157 real sec...
[M::process] read 681602 sequences (100000180 bp) in 1.330 CPU sec, 1.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278320, 8, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 242, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (281.68, 149.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (356.45, 170.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 255, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1293)
[M::mem_pestat] mean and std.dev: (326.56, 247.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1666)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682110 reads in 161.154 CPU sec, 5.138 real sec
[M::mem_process_seqs] Processed 681788 reads in 103.216 CPU sec, 3.283 real sec
sorting block 56, #reads = 1066039 mem = 536878698 peak = 1114193920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277696, 5, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 305, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (269.07, 118.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (356.41, 170.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 249, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (292.79, 107.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681262 sequences (100000295 bp) in 2.197 CPU sec, 2.341 real sec...
[M::process] read 681018 sequences (100000132 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277713, 9, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (302, 368, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (367.14, 178.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (355.79, 171.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 293, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (259.71, 97.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681548 reads in 157.617 CPU sec, 5.017 real sec
sorting block 57, #reads = 1066644 mem = 536880941 peak = 1114193920
[M::mem_process_seqs] Processed 681602 reads in 100.899 CPU sec, 3.216 real sec
[M::process] read 145506 sequences (21333026 bp) in 0.432 CPU sec, 0.450 real sec...
sorting block 58, #reads = 1068275 mem = 536878729 peak = 1139056640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277753, 9, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 237, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (242.28, 111.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (358.21, 172.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 375, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126)
[M::mem_pestat] mean and std.dev: (402.34, 236.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1415)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 278186, 8, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 205, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300)
[M::mem_pestat] mean and std.dev: (309.86, 204.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1674)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (363.48, 176.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 276, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (298.92, 131.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 59510, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (359.38, 175.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681262 reads in 166.253 CPU sec, 5.382 real sec
[M::mem_process_seqs] Processed 681018 reads in 112.667 CPU sec, 3.643 real sec
[M::mem_process_seqs] Processed 145506 reads in 116.264 CPU sec, 3.767 real sec
sorting block 59, #reads = 1067497 mem = 536886498 peak = 1139056640
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 64290078
overall: 70121103360 mem 9578.027 user 305.898 sys 1647.133 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1647.168 sec; CPU: 9883.929 sec
sorting block 60, #reads = 566226 mem = 284878443 peak = 1139056640
uniq header count 1
merging 61 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3897074880
output reads: 64669707
bam_mem_sort: 61 calls 23.254 user 0.215 sys 23.590 real
bam_write: 61 calls 49.225 user 1.153 sys 50.673 real
execute: 1 calls 12.536 user 1.084 sys 1669.057 real
merge_files: 1 calls 12.516 user 1.065 sys 21.094 real
parse_chunk: 969 calls 29.881 user 8.483 sys 39.128 real
read_chunk: 2617 calls 25.940 user 24.338 sys 271.935 real
sort_block: 1 calls 20.201 user 23.712 sys 263.474 real
write_chunk: 1646 calls 12.158 user 0.878 sys 20.322 real
overall: 2867326976 mem 790.976 user 43.001 sys 1669.079 real
ls: cannot access '*.cram': No such file or directory