cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_87O-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2060.827 CPU sec, 77.870 real sec [M::process] read 698188 sequences (100000260 bp) in 1.625 CPU sec, 1.632 real sec... [M::process] read 696414 sequences (100000079 bp) in 2.148 CPU sec, 2.270 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 286038, 4, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 226, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2421) [M::mem_pestat] mean and std.dev: (423.04, 424.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (275.99, 139.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 228, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (259.67, 101.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695678 sequences (100000197 bp) in 1.782 CPU sec, 1.839 real sec... [M::process] read 695442 sequences (100000125 bp) in 1.761 CPU sec, 1.842 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285156, 3, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 142, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (246.71, 188.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (281.21, 141.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 412, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3034) [M::mem_pestat] mean and std.dev: (495.50, 596.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698188 reads in 210.166 CPU sec, 6.840 real sec [M::mem_process_seqs] Processed 696414 reads in 125.442 CPU sec, 4.109 real sec sorting block 0, #reads = 1084649 mem = 536881117 peak = 575262720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285126, 9, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 190, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (240.80, 106.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (285.22, 144.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 227, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (257.58, 117.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694930 sequences (100000110 bp) in 2.670 CPU sec, 2.817 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284964, 2, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 192, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (182.22, 62.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (285.91, 144.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 212, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (213.71, 80.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694780 sequences (100000273 bp) in 1.859 CPU sec, 1.861 real sec... [M::mem_process_seqs] Processed 695678 reads in 181.570 CPU sec, 5.788 real sec [M::mem_process_seqs] Processed 695442 reads in 126.794 CPU sec, 4.048 real sec sorting block 1, #reads = 1085277 mem = 536881623 peak = 704237568 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284594, 6, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 201, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (220.32, 74.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (289.32, 146.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 255, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (288.02, 131.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694132 sequences (100000075 bp) in 2.839 CPU sec, 3.001 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 284607, 4, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 205, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (239.89, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (289.91, 147.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 191, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (274.70, 176.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693746 sequences (100000237 bp) in 1.906 CPU sec, 1.938 real sec... [M::mem_process_seqs] Processed 694930 reads in 184.183 CPU sec, 5.868 real sec [M::mem_process_seqs] Processed 694780 reads in 127.994 CPU sec, 4.072 real sec sorting block 2, #reads = 1084546 mem = 536877187 peak = 756482048 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284349, 9, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 242, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (222.32, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (292.30, 147.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 225, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (204.63, 62.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694006 sequences (100000295 bp) in 3.264 CPU sec, 3.478 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283888, 3, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 201, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (218.78, 92.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (293.00, 148.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 294, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (294.60, 117.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694132 reads in 182.581 CPU sec, 5.831 real sec [M::process] read 693506 sequences (100000241 bp) in 2.492 CPU sec, 2.548 real sec... sorting block 3, #reads = 1084368 mem = 536879939 peak = 756482048 [M::mem_process_seqs] Processed 693746 reads in 126.033 CPU sec, 4.010 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284067, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 165, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (177.12, 74.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (293.93, 148.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 303, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (290.70, 188.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 4, #reads = 1083864 mem = 536877114 peak = 831344640 [M::process] read 693002 sequences (100000104 bp) in 3.027 CPU sec, 3.247 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284094, 1, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 243, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (228.00, 117.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (295.16, 149.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (248.83, 109.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694006 reads in 181.319 CPU sec, 5.807 real sec [M::mem_process_seqs] Processed 693506 reads in 125.016 CPU sec, 3.966 real sec [M::process] read 692874 sequences (100000261 bp) in 2.833 CPU sec, 2.984 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284073, 1, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 143, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (204.58, 147.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (296.40, 150.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 211, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (210.28, 76.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 5, #reads = 1083131 mem = 536880878 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283733, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 207, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (212.00, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (295.08, 149.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 276, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (295.24, 155.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693092 sequences (100000253 bp) in 3.238 CPU sec, 3.498 real sec... [M::mem_process_seqs] Processed 693002 reads in 185.388 CPU sec, 5.980 real sec [M::mem_process_seqs] Processed 692874 reads in 129.440 CPU sec, 4.108 real sec sorting block 6, #reads = 1083271 mem = 536883101 peak = 831344640 [M::process] read 693066 sequences (100000206 bp) in 2.767 CPU sec, 2.983 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283731, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 183, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (191.90, 72.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (295.70, 149.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 354, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (331.14, 197.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283824, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 275, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (382.00, 242.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (294.50, 148.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 264, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (46, 446) [M::mem_pestat] mean and std.dev: (245.65, 74.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693420 sequences (100000199 bp) in 3.173 CPU sec, 3.606 real sec... [M::mem_process_seqs] Processed 693092 reads in 180.391 CPU sec, 5.761 real sec [M::mem_process_seqs] Processed 693066 reads in 126.387 CPU sec, 4.011 real sec sorting block 7, #reads = 1082788 mem = 536878115 peak = 831344640 [M::process] read 693332 sequences (100000166 bp) in 3.248 CPU sec, 3.493 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283986, 7, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 195, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (208.68, 87.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (292.57, 148.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 262, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (241.73, 74.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284040, 3, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 180, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (224.00, 88.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 267, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (292.03, 147.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 216, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (237.83, 92.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 8, #reads = 1083149 mem = 536877846 peak = 831344640 [M::process] read 693026 sequences (100000160 bp) in 3.299 CPU sec, 3.530 real sec... [M::mem_process_seqs] Processed 693420 reads in 185.149 CPU sec, 5.954 real sec [M::mem_process_seqs] Processed 693332 reads in 129.058 CPU sec, 4.122 real sec [M::process] read 693570 sequences (100000090 bp) in 3.374 CPU sec, 3.686 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284232, 2, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 258, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (235.05, 92.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 267, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (292.11, 146.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 206, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (202.58, 60.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 9, #reads = 1083631 mem = 536883140 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284017, 5, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 342, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382) [M::mem_pestat] mean and std.dev: (344.30, 191.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (292.57, 147.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 231, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (227.44, 70.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692572 sequences (100000024 bp) in 3.053 CPU sec, 3.262 real sec... [M::mem_process_seqs] Processed 693026 reads in 191.071 CPU sec, 6.165 real sec [M::mem_process_seqs] Processed 693570 reads in 131.157 CPU sec, 4.207 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283419, 3, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 176, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (199.69, 153.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (293.42, 147.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 343, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (341.00, 156.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 10, #reads = 1082551 mem = 536880986 peak = 831344640 [M::process] read 692706 sequences (100000112 bp) in 3.679 CPU sec, 3.999 real sec... [M::mem_process_seqs] Processed 692572 reads in 106.665 CPU sec, 3.542 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283810, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 197, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (216.04, 125.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (293.64, 147.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 200, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (273.96, 148.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692706 reads in 103.117 CPU sec, 3.404 real sec [M::process] read 692416 sequences (100000239 bp) in 3.774 CPU sec, 4.071 real sec... sorting block 11, #reads = 1082792 mem = 536874478 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283611, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 256, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (257.46, 131.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 270, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (294.83, 148.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 270, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2850) [M::mem_pestat] mean and std.dev: (368.28, 333.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692326 sequences (100000080 bp) in 3.167 CPU sec, 3.383 real sec... [M::mem_process_seqs] Processed 692416 reads in 103.063 CPU sec, 3.399 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283550, 5, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 219, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (224.52, 88.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 271, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (295.00, 147.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 253, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (265.86, 77.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 12, #reads = 1083712 mem = 536883684 peak = 831344640 [M::mem_process_seqs] Processed 692326 reads in 101.742 CPU sec, 3.404 real sec [M::process] read 690548 sequences (100000072 bp) in 3.408 CPU sec, 3.745 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283001, 5, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 202, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (230.21, 123.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 279, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (301.15, 149.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 204, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (204.48, 92.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690548 reads in 101.906 CPU sec, 3.356 real sec [M::process] read 691648 sequences (100000179 bp) in 3.284 CPU sec, 3.671 real sec... sorting block 13, #reads = 1082887 mem = 536878927 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283223, 5, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 175, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 362) [M::mem_pestat] mean and std.dev: (166.44, 45.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (296.00, 146.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 229, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (216.86, 46.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691648 reads in 102.413 CPU sec, 3.375 real sec [M::process] read 691558 sequences (100000205 bp) in 3.684 CPU sec, 4.047 real sec... sorting block 14, #reads = 1081581 mem = 536878897 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282694, 1, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 173, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (218.57, 115.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 274, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (296.70, 147.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 327, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (320.40, 149.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691558 reads in 101.824 CPU sec, 3.329 real sec [M::process] read 691230 sequences (100000004 bp) in 3.727 CPU sec, 4.182 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283000, 6, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 202, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (272.35, 172.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 273, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (294.66, 144.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 210, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (207.03, 87.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 15, #reads = 1081858 mem = 536879775 peak = 831344640 [M::mem_process_seqs] Processed 691230 reads in 103.266 CPU sec, 3.354 real sec [M::process] read 691212 sequences (100000238 bp) in 3.641 CPU sec, 4.013 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282972, 4, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 194, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396) [M::mem_pestat] mean and std.dev: (289.19, 190.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 275, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (297.17, 146.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 263, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (315.06, 205.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691212 reads in 105.835 CPU sec, 3.424 real sec [M::process] read 690778 sequences (100000191 bp) in 3.293 CPU sec, 3.620 real sec... sorting block 16, #reads = 1082013 mem = 536877335 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283009, 3, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 236, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (279.70, 146.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 277, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (299.27, 148.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 223, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (284.88, 153.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690778 reads in 106.177 CPU sec, 3.489 real sec [M::process] read 690524 sequences (100000024 bp) in 3.097 CPU sec, 3.545 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282930, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 224, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.92, 100.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 279, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (300.94, 148.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 289, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1410) [M::mem_pestat] mean and std.dev: (386.19, 258.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 17, #reads = 1082007 mem = 536881354 peak = 831344640 [M::mem_process_seqs] Processed 690524 reads in 106.457 CPU sec, 3.453 real sec [M::process] read 690098 sequences (100000049 bp) in 3.239 CPU sec, 3.492 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282954, 0, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 229, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (217.97, 95.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 281, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (302.98, 149.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 203, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (242.29, 112.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690098 reads in 102.014 CPU sec, 3.295 real sec [M::process] read 689862 sequences (100000034 bp) in 3.433 CPU sec, 3.744 real sec... sorting block 18, #reads = 1081061 mem = 536879445 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282772, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 253, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (257.55, 129.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 282, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (302.77, 148.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 254, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (307.77, 189.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689862 reads in 103.486 CPU sec, 3.347 real sec [M::process] read 696462 sequences (100000008 bp) in 3.084 CPU sec, 3.432 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 285303, 9, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 189, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (208.38, 99.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (282.57, 143.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 268, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309) [M::mem_pestat] mean and std.dev: (385.15, 286.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696462 reads in 103.722 CPU sec, 3.363 real sec sorting block 19, #reads = 1080219 mem = 536881981 peak = 831344640 [M::process] read 696766 sequences (100000221 bp) in 3.477 CPU sec, 3.821 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 285233, 2, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 156, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (201.50, 107.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (283.52, 144.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 223, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (251.09, 147.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695820 sequences (100000181 bp) in 2.532 CPU sec, 2.835 real sec... [M::mem_process_seqs] Processed 696766 reads in 96.743 CPU sec, 3.152 real sec sorting block 20, #reads = 1085966 mem = 536877048 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284947, 6, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 238, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131) [M::mem_pestat] mean and std.dev: (424.04, 478.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (287.27, 146.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 194, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (243.76, 132.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695698 sequences (100000212 bp) in 3.286 CPU sec, 3.547 real sec... [M::mem_process_seqs] Processed 695820 reads in 102.126 CPU sec, 3.305 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284691, 4, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (175.00, 57.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (289.28, 148.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 338, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (363.36, 180.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695142 sequences (100000134 bp) in 3.091 CPU sec, 3.289 real sec... [M::mem_process_seqs] Processed 695698 reads in 100.660 CPU sec, 3.237 real sec sorting block 21, #reads = 1086272 mem = 536879411 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284919, 7, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 187, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (195.21, 83.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (290.09, 148.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 267, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (307.18, 159.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695142 reads in 100.982 CPU sec, 3.260 real sec [M::process] read 694582 sequences (100000131 bp) in 3.512 CPU sec, 3.813 real sec... sorting block 22, #reads = 1085909 mem = 536880066 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284049, 0, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 161, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (190.38, 92.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (292.19, 149.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 247, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (224.43, 63.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694146 sequences (100000003 bp) in 2.732 CPU sec, 2.938 real sec... [M::mem_process_seqs] Processed 694582 reads in 101.088 CPU sec, 3.255 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283990, 4, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (229.27, 81.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.72, 149.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 210, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (226.35, 97.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694146 reads in 102.168 CPU sec, 3.314 real sec [M::process] read 693786 sequences (100000100 bp) in 3.789 CPU sec, 4.174 real sec... sorting block 23, #reads = 1084050 mem = 536880364 peak = 831344640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284423, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 251, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (254.90, 130.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (294.74, 150.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 223, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (266.37, 126.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693644 sequences (100000284 bp) in 2.809 CPU sec, 3.097 real sec... [M::mem_process_seqs] Processed 693786 reads in 100.099 CPU sec, 3.222 real sec sorting block 24, #reads = 1084371 mem = 536878759 peak = 836390912 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283952, 2, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 314, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (297.79, 117.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (295.38, 150.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 284, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (289.27, 141.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693156 sequences (100000270 bp) in 2.823 CPU sec, 3.039 real sec... [M::mem_process_seqs] Processed 693644 reads in 99.222 CPU sec, 3.191 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284376, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 171, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (185.57, 74.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (296.71, 150.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 214, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (205.21, 48.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693036 sequences (100000176 bp) in 3.231 CPU sec, 3.446 real sec... [M::mem_process_seqs] Processed 693156 reads in 101.636 CPU sec, 3.268 real sec sorting block 25, #reads = 1083756 mem = 536877126 peak = 836390912 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284183, 2, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 251, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (215.31, 82.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (297.23, 151.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 270, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (287.73, 147.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693036 reads in 98.806 CPU sec, 3.180 real sec [M::process] read 693044 sequences (100000242 bp) in 3.298 CPU sec, 3.521 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284046, 9, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 298, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2486) [M::mem_pestat] mean and std.dev: (478.47, 416.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (297.78, 151.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 171, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (184.60, 63.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 26, #reads = 1083629 mem = 536884924 peak = 866762752 [M::mem_process_seqs] Processed 693044 reads in 104.221 CPU sec, 3.391 real sec [M::process] read 692880 sequences (100000120 bp) in 3.460 CPU sec, 3.780 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284133, 6, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 188, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (213.64, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (296.54, 150.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 289, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (367.48, 256.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692882 sequences (100000171 bp) in 2.917 CPU sec, 3.151 real sec... [M::mem_process_seqs] Processed 692880 reads in 104.825 CPU sec, 3.411 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283663, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (195.55, 88.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (295.81, 150.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 236, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (263.26, 121.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 27, #reads = 1083554 mem = 536878574 peak = 866762752 [M::mem_process_seqs] Processed 692882 reads in 108.756 CPU sec, 3.540 real sec [M::process] read 693060 sequences (100000119 bp) in 3.729 CPU sec, 4.056 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284147, 2, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 188, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (227.50, 152.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (294.25, 148.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 231, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (264.33, 121.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693468 sequences (100000011 bp) in 2.876 CPU sec, 3.170 real sec... [M::mem_process_seqs] Processed 693060 reads in 106.973 CPU sec, 3.518 real sec sorting block 28, #reads = 1083679 mem = 536881126 peak = 866762752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284214, 4, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 203, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (232.97, 113.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.68, 149.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 200, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (253.53, 161.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693420 sequences (100000081 bp) in 3.013 CPU sec, 3.334 real sec... [M::mem_process_seqs] Processed 693468 reads in 110.087 CPU sec, 3.611 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284508, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 201, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (216.33, 94.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (294.52, 150.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 243, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (239.71, 73.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693474 sequences (100000050 bp) in 3.474 CPU sec, 3.905 real sec... [M::mem_process_seqs] Processed 693420 reads in 106.577 CPU sec, 3.484 real sec sorting block 29, #reads = 1084224 mem = 536877181 peak = 866762752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284567, 2, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (195.09, 71.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (293.08, 149.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 212, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (246.39, 104.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693696 sequences (100000144 bp) in 3.109 CPU sec, 3.473 real sec... [M::mem_process_seqs] Processed 693474 reads in 109.202 CPU sec, 3.626 real sec sorting block 30, #reads = 1083136 mem = 536879779 peak = 866762752 [M::process] read 692996 sequences (100000216 bp) in 2.805 CPU sec, 2.984 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284281, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 201, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (182.41, 64.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 267, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (292.87, 148.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 201, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (227.74, 106.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284103, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 242, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (211.32, 73.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (294.46, 148.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 224, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (287.29, 197.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692772 sequences (100000094 bp) in 2.651 CPU sec, 2.886 real sec... [M::mem_process_seqs] Processed 693696 reads in 186.072 CPU sec, 5.968 real sec [M::mem_process_seqs] Processed 692996 reads in 128.852 CPU sec, 4.103 real sec sorting block 31, #reads = 1083012 mem = 536882143 peak = 866762752 [M::process] read 692872 sequences (100000092 bp) in 3.390 CPU sec, 3.656 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284059, 1, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 192, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (180.87, 62.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (294.47, 148.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 254, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (303.89, 152.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692772 reads in 101.826 CPU sec, 3.394 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283735, 1, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 190, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (232.06, 106.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 268, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (292.88, 147.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 327, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (344.21, 143.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692510 sequences (100000087 bp) in 2.981 CPU sec, 3.196 real sec... sorting block 32, #reads = 1083001 mem = 536876461 peak = 866762752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283772, 11, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 266, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (235.97, 102.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (294.93, 148.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (742, 1190, 2350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5566) [M::mem_pestat] mean and std.dev: (1488.73, 831.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 275, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (283.23, 153.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692510 reads in 103.025 CPU sec, 3.340 real sec [M::process] read 692286 sequences (100000001 bp) in 3.575 CPU sec, 3.992 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284233, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 237, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (273.49, 135.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (296.80, 149.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 272, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (250.59, 115.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 33, #reads = 1082827 mem = 536879694 peak = 869978112 [M::mem_process_seqs] Processed 692286 reads in 101.001 CPU sec, 3.284 real sec [M::process] read 692138 sequences (100000096 bp) in 3.015 CPU sec, 3.289 real sec... [M::mem_process_seqs] Processed 692872 reads in 303.217 CPU sec, 10.343 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283489, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 154, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (191.42, 106.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 273, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (297.22, 149.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 253, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (241.03, 83.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692138 reads in 101.590 CPU sec, 3.398 real sec [M::process] read 691874 sequences (100000080 bp) in 3.599 CPU sec, 3.991 real sec... sorting block 34, #reads = 1082363 mem = 536882361 peak = 869978112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283964, 0, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 321, 2536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7166) [M::mem_pestat] mean and std.dev: (1375.38, 1916.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 273, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (297.99, 150.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 198, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (226.47, 94.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691874 reads in 102.276 CPU sec, 3.433 real sec [M::process] read 691956 sequences (100000086 bp) in 3.177 CPU sec, 3.459 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283684, 6, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 186, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 317) [M::mem_pestat] mean and std.dev: (192.18, 50.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (295.81, 147.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 197, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (210.42, 70.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691760 sequences (100000218 bp) in 3.106 CPU sec, 3.370 real sec... [M::mem_process_seqs] Processed 691956 reads in 101.650 CPU sec, 3.392 real sec sorting block 35, #reads = 1082474 mem = 536887091 peak = 870694912 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283977, 3, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 204, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (238.03, 144.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 274, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (297.33, 148.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (213.56, 51.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691060 sequences (100000102 bp) in 3.037 CPU sec, 3.293 real sec... [M::mem_process_seqs] Processed 691760 reads in 100.871 CPU sec, 3.400 real sec sorting block 36, #reads = 1082287 mem = 536876985 peak = 870694912 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283498, 6, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 222, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (222.11, 114.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 276, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (298.61, 148.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 210, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (219.56, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691060 reads in 99.219 CPU sec, 3.328 real sec [M::process] read 690756 sequences (100000010 bp) in 3.415 CPU sec, 3.713 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283628, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 240, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (264.00, 173.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (302.42, 151.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 240, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (258.21, 100.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690756 reads in 101.833 CPU sec, 3.410 real sec sorting block 37, #reads = 1081939 mem = 536880984 peak = 870694912 [M::process] read 689510 sequences (100000190 bp) in 3.635 CPU sec, 4.055 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282551, 0, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 223, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (238.82, 113.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 285, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (307.30, 152.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 245, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (230.28, 72.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689510 reads in 100.723 CPU sec, 3.364 real sec [M::process] read 695840 sequences (100000052 bp) in 3.641 CPU sec, 4.048 real sec... sorting block 38, #reads = 1081684 mem = 536879180 peak = 870694912 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284951, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 250, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (243.24, 118.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (286.32, 145.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 227, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (251.59, 149.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695840 reads in 105.259 CPU sec, 3.508 real sec [M::process] read 696914 sequences (100000107 bp) in 3.323 CPU sec, 3.573 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 285870, 0, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 214, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (215.09, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 256, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (282.16, 143.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 336, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (362.74, 181.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696914 reads in 99.790 CPU sec, 3.351 real sec [M::process] read 695942 sequences (100000071 bp) in 3.310 CPU sec, 3.606 real sec... sorting block 39, #reads = 1080054 mem = 536880739 peak = 870694912 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285469, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 221, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (236.04, 97.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 260, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (284.67, 143.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 168, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (164.50, 56.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695942 reads in 102.804 CPU sec, 3.435 real sec [M::process] read 695328 sequences (100000112 bp) in 3.671 CPU sec, 4.093 real sec... sorting block 40, #reads = 1084460 mem = 536880122 peak = 870694912 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285113, 0, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 210, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (216.74, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (286.53, 144.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 299, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (302.61, 159.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695328 reads in 102.873 CPU sec, 3.438 real sec [M::process] read 694678 sequences (100000202 bp) in 3.395 CPU sec, 3.819 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284432, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 199, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (236.80, 100.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (288.80, 145.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 271, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (242.90, 114.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694198 sequences (100000049 bp) in 2.784 CPU sec, 3.046 real sec... [M::mem_process_seqs] Processed 694678 reads in 105.211 CPU sec, 3.543 real sec sorting block 41, #reads = 1084581 mem = 536880627 peak = 870694912 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284168, 1, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 283, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (257.48, 95.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (289.94, 145.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 229, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (246.24, 103.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694198 reads in 102.714 CPU sec, 3.447 real sec [M::process] read 694298 sequences (100000284 bp) in 3.437 CPU sec, 3.792 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284737, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (203.93, 73.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (290.72, 145.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (190.08, 70.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694298 reads in 105.468 CPU sec, 3.535 real sec sorting block 42, #reads = 1084021 mem = 536879187 peak = 875470848 [M::process] read 693374 sequences (100000152 bp) in 3.546 CPU sec, 3.887 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284226, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 216, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 352) [M::mem_pestat] mean and std.dev: (193.70, 46.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (291.35, 144.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 238, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (200.88, 54.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693620 sequences (100000276 bp) in 3.017 CPU sec, 3.241 real sec... [M::mem_process_seqs] Processed 693374 reads in 104.662 CPU sec, 3.513 real sec sorting block 43, #reads = 1083390 mem = 536879714 peak = 898580480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283687, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 308, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2117) [M::mem_pestat] mean and std.dev: (420.72, 363.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (293.06, 146.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1103, 2605, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5609) [M::mem_pestat] mean and std.dev: (1976.60, 987.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 215, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (261.10, 112.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693620 reads in 105.363 CPU sec, 3.534 real sec [M::process] read 693000 sequences (100000196 bp) in 3.704 CPU sec, 4.099 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284088, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 189, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (243.17, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (295.05, 147.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 291, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (244.77, 79.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693000 reads in 103.144 CPU sec, 3.456 real sec sorting block 44, #reads = 1082281 mem = 536877565 peak = 898580480 [M::process] read 692594 sequences (100000242 bp) in 3.397 CPU sec, 3.768 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283803, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (229.79, 113.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 273, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (296.34, 148.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 253, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (238.44, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692594 reads in 103.732 CPU sec, 3.486 real sec [M::process] read 692542 sequences (100000293 bp) in 3.558 CPU sec, 3.944 real sec... sorting block 45, #reads = 1083249 mem = 536879350 peak = 898580480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283298, 1, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (193.26, 69.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (298.06, 150.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 242, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (253.55, 113.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692542 reads in 102.210 CPU sec, 3.432 real sec [M::process] read 692514 sequences (100000134 bp) in 3.190 CPU sec, 3.532 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283497, 0, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 225, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (245.81, 116.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (297.61, 149.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 310, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419) [M::mem_pestat] mean and std.dev: (393.56, 302.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692340 sequences (100000013 bp) in 2.977 CPU sec, 3.235 real sec... [M::mem_process_seqs] Processed 692514 reads in 106.656 CPU sec, 3.591 real sec sorting block 46, #reads = 1082276 mem = 536878313 peak = 898580480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283636, 5, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 157, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (192.48, 91.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 273, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (297.34, 149.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (257.23, 113.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692340 reads in 104.796 CPU sec, 3.508 real sec [M::process] read 692556 sequences (100000202 bp) in 3.298 CPU sec, 3.599 real sec... sorting block 47, #reads = 1083208 mem = 536885303 peak = 898580480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284216, 3, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 265, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (256.12, 131.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (296.52, 149.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 333, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1375) [M::mem_pestat] mean and std.dev: (397.26, 311.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1783) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692556 reads in 102.290 CPU sec, 3.441 real sec [M::process] read 692652 sequences (100000042 bp) in 3.399 CPU sec, 3.725 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283704, 4, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 343, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (386.20, 253.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (294.61, 148.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 269, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (287.27, 125.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693118 sequences (100000062 bp) in 3.229 CPU sec, 3.519 real sec... [M::mem_process_seqs] Processed 692652 reads in 106.118 CPU sec, 3.548 real sec sorting block 48, #reads = 1082188 mem = 536887391 peak = 898580480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284121, 1, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 249, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (249.17, 109.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (292.81, 147.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 255, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (262.57, 130.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1422) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693118 reads in 100.988 CPU sec, 3.356 real sec [M::process] read 692934 sequences (100000067 bp) in 3.272 CPU sec, 3.533 real sec... sorting block 49, #reads = 1082295 mem = 536880646 peak = 898580480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283565, 4, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 154, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (176.75, 68.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (294.57, 148.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 214, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (244.73, 114.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692934 reads in 103.869 CPU sec, 3.461 real sec [M::process] read 692856 sequences (100000239 bp) in 3.391 CPU sec, 3.744 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283800, 3, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 298, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (295.89, 119.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 270, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (294.74, 148.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 210, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (209.44, 94.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692856 reads in 102.095 CPU sec, 3.405 real sec [M::process] read 692804 sequences (100000233 bp) in 3.175 CPU sec, 3.482 real sec... sorting block 50, #reads = 1082929 mem = 536879275 peak = 898580480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283532, 11, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 282, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (315.89, 166.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (293.92, 147.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (904, 1168, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (376, 1696) [M::mem_pestat] mean and std.dev: (1130.29, 92.38) [M::mem_pestat] low and high boundaries for proper pairs: (112, 1960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 220, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (231.10, 107.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692804 reads in 104.191 CPU sec, 3.469 real sec [M::process] read 692560 sequences (100000061 bp) in 3.483 CPU sec, 3.768 real sec... sorting block 51, #reads = 1082366 mem = 536878934 peak = 905154560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283461, 2, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 229, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (213.46, 75.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 270, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (294.07, 147.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 265, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (284.44, 140.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692560 reads in 99.606 CPU sec, 3.306 real sec [M::process] read 692476 sequences (100000193 bp) in 3.232 CPU sec, 3.602 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283673, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 196, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (216.43, 76.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (296.25, 149.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 242, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (241.97, 99.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692476 reads in 100.918 CPU sec, 3.343 real sec sorting block 52, #reads = 1081553 mem = 536883585 peak = 905154560 [M::process] read 692196 sequences (100000147 bp) in 3.466 CPU sec, 3.817 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283547, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 307, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3082) [M::mem_pestat] mean and std.dev: (525.40, 451.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (296.73, 149.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 313, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1606) [M::mem_pestat] mean and std.dev: (381.13, 211.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692196 reads in 98.744 CPU sec, 3.256 real sec [M::process] read 692210 sequences (100000013 bp) in 3.240 CPU sec, 3.608 real sec... sorting block 53, #reads = 1082060 mem = 536879731 peak = 905154560 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283762, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (245.41, 146.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (296.80, 149.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 201, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (279.59, 217.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692210 reads in 100.498 CPU sec, 3.286 real sec [M::process] read 691984 sequences (100000063 bp) in 3.077 CPU sec, 3.479 real sec... sorting block 54, #reads = 1082404 mem = 536884267 peak = 943161344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283303, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 244, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (243.64, 106.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 273, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (296.94, 149.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 230, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (257.74, 105.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691984 reads in 98.283 CPU sec, 3.205 real sec [M::process] read 692142 sequences (100000201 bp) in 3.419 CPU sec, 3.772 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283697, 2, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 223, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (247.61, 161.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (298.44, 150.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 271, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (266.77, 131.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 55, #reads = 1082549 mem = 536879002 peak = 943161344 [M::mem_process_seqs] Processed 692142 reads in 99.782 CPU sec, 3.224 real sec [M::process] read 691444 sequences (100000077 bp) in 3.464 CPU sec, 3.830 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282884, 5, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 242, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (251.75, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 275, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (299.41, 150.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 234, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (282.21, 128.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691444 reads in 93.740 CPU sec, 3.016 real sec [M::process] read 691222 sequences (100000284 bp) in 2.963 CPU sec, 3.206 real sec... sorting block 56, #reads = 1082507 mem = 536878785 peak = 943161344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283081, 4, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 228, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (217.40, 96.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 278, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (300.66, 150.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 232, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (276.69, 148.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691222 reads in 95.777 CPU sec, 3.113 real sec [M::process] read 690718 sequences (100000031 bp) in 3.166 CPU sec, 3.463 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282991, 1, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 187, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (218.03, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (302.22, 150.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 262, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (283.59, 91.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690718 reads in 96.317 CPU sec, 3.112 real sec sorting block 57, #reads = 1082435 mem = 536879633 peak = 943161344 [M::process] read 690368 sequences (100000087 bp) in 3.028 CPU sec, 3.362 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282528, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (246.84, 217.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 280, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (302.39, 149.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 272, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (295.03, 186.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690368 reads in 94.001 CPU sec, 3.037 real sec [M::process] read 689614 sequences (100000071 bp) in 3.625 CPU sec, 3.928 real sec... sorting block 58, #reads = 1081689 mem = 536877588 peak = 943161344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282407, 10, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 208, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 368) [M::mem_pestat] mean and std.dev: (186.39, 37.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 283, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (305.40, 151.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 1683, 3364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9694) [M::mem_pestat] mean and std.dev: (2252.20, 2405.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 292, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (248.28, 83.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696608 sequences (100000196 bp) in 2.526 CPU sec, 2.691 real sec... [M::process] read 697276 sequences (100000174 bp) in 1.530 CPU sec, 1.527 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285377, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 154, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (224.37, 113.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (283.37, 144.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 248, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (289.70, 163.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696594 sequences (100000140 bp) in 1.440 CPU sec, 1.447 real sec... [M::mem_process_seqs] Processed 696608 reads in 96.667 CPU sec, 3.041 real sec [M::mem_process_seqs] Processed 689614 reads in 215.668 CPU sec, 6.875 real sec sorting block 59, #reads = 1084771 mem = 536878149 peak = 943161344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284861, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 306, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (271.90, 115.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (281.93, 145.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 274, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (219.00, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285215, 1, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 315, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (294.47, 98.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (286.55, 148.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 242, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (256.93, 145.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695712 sequences (100000031 bp) in 2.946 CPU sec, 3.136 real sec... [M::mem_process_seqs] Processed 697276 reads in 163.776 CPU sec, 5.194 real sec [M::mem_process_seqs] Processed 696594 reads in 95.215 CPU sec, 3.005 real sec sorting block 60, #reads = 1081903 mem = 536878412 peak = 943161344 [M::process] read 695492 sequences (100000156 bp) in 2.612 CPU sec, 2.851 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284614, 5, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 231, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (228.25, 92.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (288.09, 148.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 228, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (221.49, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695048 sequences (100000282 bp) in 1.693 CPU sec, 1.699 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284572, 2, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 229, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (242.94, 128.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (288.68, 147.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 310, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (291.48, 100.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695712 reads in 144.725 CPU sec, 4.581 real sec sorting block 61, #reads = 1086600 mem = 536879448 peak = 990908416 [M::process] read 694964 sequences (100000117 bp) in 2.533 CPU sec, 2.671 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284885, 4, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 168, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (187.74, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (290.62, 148.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 354, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (284.24, 122.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695048 reads in 87.773 CPU sec, 2.784 real sec sorting block 62, #reads = 1084857 mem = 536879868 peak = 990908416 [M::process] read 694368 sequences (100000289 bp) in 3.145 CPU sec, 3.315 real sec... [M::mem_process_seqs] Processed 695492 reads in 255.079 CPU sec, 8.042 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284646, 3, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 174, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (188.17, 53.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (291.14, 149.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 297, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (270.14, 108.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284762, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 351, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (306.19, 196.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (293.03, 149.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 264, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (266.62, 127.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694964 reads in 172.150 CPU sec, 5.442 real sec [M::mem_process_seqs] Processed 694368 reads in 105.131 CPU sec, 3.328 real sec sorting block 63, #reads = 1085181 mem = 536878211 peak = 990908416 [M::process] read 693970 sequences (100000137 bp) in 3.497 CPU sec, 3.814 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284490, 2, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 220, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (291.23, 138.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (293.79, 149.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 290, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (287.15, 131.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693970 reads in 90.478 CPU sec, 2.953 real sec sorting block 64, #reads = 1084644 mem = 536878524 peak = 990908416 [M::process] read 693818 sequences (100000026 bp) in 3.408 CPU sec, 3.687 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283864, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 287, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (359.85, 228.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.58, 149.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 183, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (205.16, 80.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693818 reads in 90.775 CPU sec, 2.919 real sec [M::process] read 695398 sequences (100000220 bp) in 2.942 CPU sec, 3.137 real sec... sorting block 65, #reads = 1085304 mem = 536877975 peak = 990908416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284982, 8, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 322, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (327.14, 171.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (289.68, 148.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 311, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108) [M::mem_pestat] mean and std.dev: (313.24, 199.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1418) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695398 reads in 88.215 CPU sec, 2.847 real sec [M::process] read 693302 sequences (100000023 bp) in 3.344 CPU sec, 3.608 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284058, 3, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 265, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 453) [M::mem_pestat] mean and std.dev: (234.31, 70.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (296.19, 150.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 243, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (204.00, 64.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693016 sequences (100000269 bp) in 2.280 CPU sec, 2.408 real sec... [M::mem_process_seqs] Processed 693302 reads in 90.645 CPU sec, 2.913 real sec sorting block 66, #reads = 1085231 mem = 536878046 peak = 990908416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283997, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 221, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (242.42, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (296.13, 149.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 216, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 447) [M::mem_pestat] mean and std.dev: (227.00, 75.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693164 sequences (100000195 bp) in 2.681 CPU sec, 2.858 real sec... [M::mem_process_seqs] Processed 693016 reads in 93.087 CPU sec, 2.997 real sec sorting block 67, #reads = 1083975 mem = 536877327 peak = 990908416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284897, 13, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 270, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (256.10, 114.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (296.84, 150.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (428, 2500, 2500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6644) [M::mem_pestat] mean and std.dev: (1565.69, 1011.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (222.14, 93.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692934 sequences (100000287 bp) in 2.816 CPU sec, 3.002 real sec... [M::mem_process_seqs] Processed 693164 reads in 92.004 CPU sec, 2.957 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283529, 3, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 187, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (207.30, 111.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (295.85, 149.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 262, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (235.11, 110.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692490 sequences (100000099 bp) in 2.693 CPU sec, 2.888 real sec... [M::mem_process_seqs] Processed 692934 reads in 91.064 CPU sec, 2.924 real sec sorting block 68, #reads = 1084510 mem = 536877902 peak = 990908416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283731, 8, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 237, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (83, 448) [M::mem_pestat] mean and std.dev: (256.68, 65.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 270, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (294.91, 148.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 169, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 329) [M::mem_pestat] mean and std.dev: (170.89, 43.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693136 sequences (100000054 bp) in 2.912 CPU sec, 3.157 real sec... [M::mem_process_seqs] Processed 692490 reads in 91.486 CPU sec, 2.939 real sec sorting block 69, #reads = 1082973 mem = 536879274 peak = 990908416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283790, 7, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 176, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (169.27, 67.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (294.27, 148.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 187, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (216.35, 74.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692792 sequences (100000217 bp) in 2.779 CPU sec, 2.959 real sec... [M::mem_process_seqs] Processed 693136 reads in 91.217 CPU sec, 2.926 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283907, 5, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 293, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (254.54, 105.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (293.78, 148.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 259, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (261.76, 104.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693210 sequences (100000197 bp) in 2.702 CPU sec, 2.917 real sec... [M::mem_process_seqs] Processed 692792 reads in 90.728 CPU sec, 2.927 real sec sorting block 70, #reads = 1082470 mem = 536883447 peak = 990908416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283898, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 292, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (385.90, 319.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (294.61, 149.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 299, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (53, 508) [M::mem_pestat] mean and std.dev: (278.30, 80.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692948 sequences (100000126 bp) in 2.803 CPU sec, 3.030 real sec... [M::mem_process_seqs] Processed 693210 reads in 94.208 CPU sec, 3.028 real sec sorting block 71, #reads = 1083096 mem = 536878231 peak = 990908416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283665, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 217, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 444) [M::mem_pestat] mean and std.dev: (207.84, 64.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (294.29, 148.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 178, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (222.07, 128.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693070 sequences (100000099 bp) in 2.766 CPU sec, 2.911 real sec... [M::mem_process_seqs] Processed 692948 reads in 83.899 CPU sec, 2.691 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283717, 4, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (182.47, 51.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (292.96, 147.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 232, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (262.89, 134.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693070 reads in 79.308 CPU sec, 2.599 real sec [M::process] read 692680 sequences (100000125 bp) in 3.080 CPU sec, 3.298 real sec... sorting block 72, #reads = 1083752 mem = 536879142 peak = 990908416 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283700, 3, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 182, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (256.53, 141.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (294.05, 147.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 216, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (274.00, 153.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692680 reads in 78.421 CPU sec, 2.515 real sec [M::process] read 692338 sequences (100000244 bp) in 2.527 CPU sec, 2.636 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283474, 0, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (186.29, 61.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (294.50, 147.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 239, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (272.86, 160.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1316) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 73, #reads = 1082264 mem = 536881049 peak = 990908416 [M::mem_process_seqs] Processed 692338 reads in 79.207 CPU sec, 2.555 real sec [M::process] read 691916 sequences (100000038 bp) in 3.230 CPU sec, 3.612 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284046, 3, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 204, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2604) [M::mem_pestat] mean and std.dev: (363.87, 337.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 273, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (296.61, 148.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 223, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (214.38, 87.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691716 sequences (100000293 bp) in 1.932 CPU sec, 2.005 real sec... [M::process] read 691892 sequences (100000003 bp) in 1.400 CPU sec, 1.394 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283897, 6, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 191, 1698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4798) [M::mem_pestat] mean and std.dev: (607.10, 662.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 273, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (296.43, 147.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 230, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (210.96, 92.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691772 sequences (100000212 bp) in 1.300 CPU sec, 1.307 real sec... [M::mem_process_seqs] Processed 691716 reads in 76.303 CPU sec, 2.400 real sec sorting block 74, #reads = 1081639 mem = 536879806 peak = 990908416 [M::mem_process_seqs] Processed 691916 reads in 182.460 CPU sec, 5.799 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283894, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 221, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.75, 64.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 272, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (294.74, 145.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 303, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 483) [M::mem_pestat] mean and std.dev: (254.61, 80.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691326 sequences (100000114 bp) in 2.707 CPU sec, 2.968 real sec... [M::mem_process_seqs] Processed 691772 reads in 74.453 CPU sec, 2.386 real sec sorting block 75, #reads = 1082097 mem = 536883048 peak = 993796096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283358, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 210, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (208.75, 102.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 272, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (294.90, 146.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 298, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (301.91, 157.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691386 sequences (100000061 bp) in 2.116 CPU sec, 2.236 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284393, 8, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 172, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (96, 236) [M::mem_pestat] mean and std.dev: (155.40, 21.06) [M::mem_pestat] low and high boundaries for proper pairs: (68, 264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 276, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (297.61, 147.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 154, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (304.35, 276.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691892 reads in 223.973 CPU sec, 7.129 real sec [M::mem_process_seqs] Processed 691326 reads in 91.219 CPU sec, 2.879 real sec sorting block 76, #reads = 1082752 mem = 536879773 peak = 1052729344 [M::process] read 690514 sequences (100000297 bp) in 2.656 CPU sec, 2.807 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283090, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 204, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (216.48, 112.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 276, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (299.03, 148.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 176, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (179.24, 51.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283194, 2, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 213, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (205.08, 56.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (302.61, 150.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 254, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (266.34, 85.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691386 reads in 134.899 CPU sec, 4.276 real sec [M::process] read 690116 sequences (100000269 bp) in 2.645 CPU sec, 2.901 real sec... sorting block 77, #reads = 1081857 mem = 536882963 peak = 1052729344 [M::mem_process_seqs] Processed 690514 reads in 92.767 CPU sec, 2.931 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283063, 15, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 199, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (201.25, 57.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 283, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (305.14, 151.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (703, 703, 2483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6043) [M::mem_pestat] mean and std.dev: (1331.80, 924.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 219, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (276.62, 150.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690116 reads in 76.871 CPU sec, 2.527 real sec sorting block 78, #reads = 1080558 mem = 536881154 peak = 1052729344 [M::process] read 700808 sequences (100000195 bp) in 3.609 CPU sec, 3.944 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 286537, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 153, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (179.89, 73.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (272.29, 143.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 341, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192) [M::mem_pestat] mean and std.dev: (317.32, 146.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700808 reads in 83.153 CPU sec, 2.676 real sec [M::process] read 700478 sequences (100000223 bp) in 2.672 CPU sec, 2.837 real sec... sorting block 79, #reads = 1079922 mem = 536877362 peak = 1052729344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 287280, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 314, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497) [M::mem_pestat] mean and std.dev: (358.28, 201.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (273.92, 144.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 221, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (55, 375) [M::mem_pestat] mean and std.dev: (223.86, 73.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700478 reads in 81.148 CPU sec, 2.606 real sec [M::process] read 699354 sequences (100000026 bp) in 2.575 CPU sec, 2.743 real sec... sorting block 80, #reads = 1086963 mem = 536878528 peak = 1052729344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 286478, 3, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 211, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (262.29, 142.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (278.16, 146.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 247, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (208.15, 65.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699354 reads in 78.239 CPU sec, 2.516 real sec [M::process] read 698612 sequences (100000157 bp) in 2.911 CPU sec, 3.153 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286309, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 190, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (208.43, 93.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (280.21, 146.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 258, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (238.41, 85.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698612 reads in 78.828 CPU sec, 2.530 real sec [M::process] read 698154 sequences (100000025 bp) in 2.598 CPU sec, 2.818 real sec... sorting block 81, #reads = 1086774 mem = 536878900 peak = 1052729344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286162, 7, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 235, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (282.45, 133.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (281.59, 146.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 160, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (178.79, 70.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698154 reads in 79.166 CPU sec, 2.546 real sec [M::process] read 697328 sequences (100000071 bp) in 3.255 CPU sec, 3.467 real sec... sorting block 82, #reads = 1084907 mem = 536877161 peak = 1052729344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285894, 5, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 238, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (270.00, 138.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (285.13, 148.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 263, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2454) [M::mem_pestat] mean and std.dev: (326.57, 340.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696956 sequences (100000124 bp) in 1.982 CPU sec, 2.046 real sec... [M::mem_process_seqs] Processed 697328 reads in 77.374 CPU sec, 2.478 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285715, 2, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 169, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (197.66, 80.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (286.41, 148.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 257, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 409) [M::mem_pestat] mean and std.dev: (229.82, 57.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696562 sequences (100000187 bp) in 2.765 CPU sec, 2.941 real sec... [M::mem_process_seqs] Processed 696956 reads in 84.491 CPU sec, 2.708 real sec sorting block 83, #reads = 1085408 mem = 536878369 peak = 1052729344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 285733, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 216, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (216.81, 105.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (288.59, 150.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (220.70, 101.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696562 reads in 75.924 CPU sec, 2.436 real sec [M::process] read 695756 sequences (100000151 bp) in 2.599 CPU sec, 2.770 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285004, 4, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 184, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 431) [M::mem_pestat] mean and std.dev: (200.13, 68.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (291.06, 151.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 228, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 398) [M::mem_pestat] mean and std.dev: (211.32, 67.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 84, #reads = 1082418 mem = 536876892 peak = 1052729344 [M::mem_process_seqs] Processed 695756 reads in 75.775 CPU sec, 2.438 real sec [M::process] read 695700 sequences (100000239 bp) in 2.524 CPU sec, 2.831 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285500, 1, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 213, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (196.92, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (291.22, 151.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 318, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (259.10, 117.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695588 sequences (100000239 bp) in 2.209 CPU sec, 2.290 real sec... [M::mem_process_seqs] Processed 695700 reads in 77.362 CPU sec, 2.484 real sec sorting block 85, #reads = 1084260 mem = 536877897 peak = 1052729344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 285226, 5, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 237, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 439) [M::mem_pestat] mean and std.dev: (226.00, 53.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (292.61, 151.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 355, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (381.18, 199.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695588 reads in 78.638 CPU sec, 2.524 real sec [M::process] read 695344 sequences (100000267 bp) in 3.110 CPU sec, 3.370 real sec... [M::process] read 694610 sequences (100000049 bp) in 1.927 CPU sec, 1.979 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285107, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 210, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (218.64, 117.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (292.42, 151.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 275, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (245.27, 128.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694014 sequences (100000120 bp) in 1.519 CPU sec, 1.524 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285176, 5, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 255, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (253.10, 116.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (295.51, 152.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 236, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (293.28, 167.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695344 reads in 136.994 CPU sec, 4.323 real sec sorting block 86, #reads = 1083269 mem = 536878728 peak = 1052729344 [M::mem_process_seqs] Processed 694610 reads in 91.142 CPU sec, 2.865 real sec [M::process] read 694222 sequences (100000140 bp) in 2.213 CPU sec, 2.340 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284294, 2, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (154.62, 64.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (295.45, 152.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 192, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 402) [M::mem_pestat] mean and std.dev: (201.55, 43.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 87, #reads = 1082905 mem = 536878400 peak = 1052729344 [M::process] read 693844 sequences (100000222 bp) in 2.168 CPU sec, 2.296 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284610, 12, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 258, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (236.14, 108.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (295.39, 152.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 342, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2506) [M::mem_pestat] mean and std.dev: (400.17, 363.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 249, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (195, 285) [M::mem_pestat] mean and std.dev: (243.20, 10.60) [M::mem_pestat] low and high boundaries for proper pairs: (177, 303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694014 reads in 135.499 CPU sec, 4.325 real sec [M::mem_process_seqs] Processed 694222 reads in 93.356 CPU sec, 2.934 real sec sorting block 88, #reads = 1081963 mem = 536883408 peak = 1052729344 [M::process] read 693830 sequences (100000282 bp) in 2.066 CPU sec, 2.156 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284142, 2, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (204.09, 89.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (295.10, 151.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 270, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (232.62, 83.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693702 sequences (100000032 bp) in 1.939 CPU sec, 2.014 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283959, 9, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 175, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (200.74, 110.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (295.10, 150.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 312, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1597) [M::mem_pestat] mean and std.dev: (399.03, 328.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693844 reads in 133.146 CPU sec, 4.202 real sec [M::process] read 693970 sequences (100000178 bp) in 1.564 CPU sec, 1.602 real sec... [M::mem_process_seqs] Processed 693830 reads in 89.434 CPU sec, 2.810 real sec sorting block 89, #reads = 1081614 mem = 536884309 peak = 1052729344 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284224, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 231, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (225.95, 87.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (295.73, 151.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 248, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (254.72, 126.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693864 sequences (100000279 bp) in 2.167 CPU sec, 2.271 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284214, 4, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 277, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 397) [M::mem_pestat] mean and std.dev: (237.24, 56.80) [M::mem_pestat] low and high boundaries for proper pairs: (10, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (294.10, 150.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 206, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 435) [M::mem_pestat] mean and std.dev: (222.37, 67.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693702 reads in 134.990 CPU sec, 4.291 real sec [M::mem_process_seqs] Processed 693970 reads in 92.178 CPU sec, 2.899 real sec sorting block 90, #reads = 1082275 mem = 536878863 peak = 1052729344 [M::process] read 693496 sequences (100000028 bp) in 1.951 CPU sec, 2.001 real sec... sorting block 91, #reads = 1081156 mem = 536883056 peak = 1090076672 [M::process] read 692586 sequences (100000295 bp) in 2.232 CPU sec, 2.378 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284226, 2, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 243, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (223.70, 96.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (295.21, 150.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 255, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (222.77, 89.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693496 reads in 76.431 CPU sec, 2.407 real sec [M::process] read 693264 sequences (100000268 bp) in 2.013 CPU sec, 2.096 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283887, 6, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 160, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (141.67, 42.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (293.74, 149.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 221, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (255.38, 113.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284413, 1, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 239, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (240.25, 69.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 273, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (297.62, 149.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 231, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (249.12, 144.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693864 reads in 210.867 CPU sec, 6.697 real sec [M::mem_process_seqs] Processed 692586 reads in 109.576 CPU sec, 3.461 real sec sorting block 92, #reads = 1080367 mem = 536878812 peak = 1090076672 [M::process] read 693138 sequences (100000177 bp) in 2.266 CPU sec, 2.396 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283694, 0, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 267, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1606) [M::mem_pestat] mean and std.dev: (417.50, 350.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (294.30, 148.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 296, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (297.38, 171.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 93, #reads = 1079916 mem = 536880993 peak = 1090076672 [M::process] read 693126 sequences (100000109 bp) in 2.270 CPU sec, 2.488 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283916, 6, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 176, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (195.62, 77.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (293.55, 147.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 297, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (280.37, 122.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693264 reads in 135.806 CPU sec, 4.327 real sec [M::mem_process_seqs] Processed 693138 reads in 91.696 CPU sec, 2.892 real sec [M::process] read 693040 sequences (100000070 bp) in 2.365 CPU sec, 2.539 real sec... sorting block 94, #reads = 1080253 mem = 536878346 peak = 1090076672 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284191, 2, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 256, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (220.65, 84.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (294.23, 147.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 203, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3287) [M::mem_pestat] mean and std.dev: (411.67, 395.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4322) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693132 sequences (100000282 bp) in 2.336 CPU sec, 2.587 real sec... [M::mem_process_seqs] Processed 693040 reads in 77.429 CPU sec, 2.479 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284368, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 258, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (252.24, 109.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 95, #reads = 1079416 mem = 536877515 peak = 1090076672 [M::mem_pestat] (25, 50, 75) percentile: (176, 268, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (292.31, 146.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 263, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (329.68, 215.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283893, 11, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 287, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (314.59, 156.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (294.87, 148.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 515, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2958) [M::mem_pestat] mean and std.dev: (746.00, 888.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (237.00, 129.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692878 sequences (100000189 bp) in 2.323 CPU sec, 2.470 real sec... [M::mem_process_seqs] Processed 693126 reads in 214.049 CPU sec, 6.838 real sec [M::mem_process_seqs] Processed 693132 reads in 94.124 CPU sec, 2.979 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284055, 4, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 181, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (209.35, 132.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (295.32, 149.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 298, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (261.64, 83.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692186 sequences (100000013 bp) in 2.896 CPU sec, 3.140 real sec... [M::mem_process_seqs] Processed 692878 reads in 78.486 CPU sec, 2.569 real sec sorting block 96, #reads = 1080386 mem = 536877303 peak = 1090076672 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283524, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (176.17, 67.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (298.47, 151.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (241.48, 127.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692186 reads in 79.714 CPU sec, 2.589 real sec [M::process] read 701884 sequences (100000276 bp) in 2.858 CPU sec, 3.154 real sec... sorting block 97, #reads = 1079735 mem = 536880131 peak = 1090076672 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 287190, 6, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 176, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (198.14, 92.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 240, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (269.40, 140.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 196, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (229.71, 106.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 701774 sequences (100000167 bp) in 2.225 CPU sec, 2.307 real sec... [M::mem_process_seqs] Processed 701884 reads in 84.370 CPU sec, 2.707 real sec sorting block 98, #reads = 1079587 mem = 536879772 peak = 1090076672 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 287122, 3, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 232, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (286.10, 187.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (268.46, 139.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 219, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (257.12, 122.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 700436 sequences (100000289 bp) in 2.526 CPU sec, 2.711 real sec... [M::mem_process_seqs] Processed 701774 reads in 81.631 CPU sec, 2.619 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 287084, 2, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 241, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 413) [M::mem_pestat] mean and std.dev: (237.80, 76.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (272.91, 141.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 212, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (232.04, 103.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 699024 sequences (100000131 bp) in 2.377 CPU sec, 2.559 real sec... [M::mem_process_seqs] Processed 700436 reads in 79.014 CPU sec, 2.533 real sec sorting block 99, #reads = 1083787 mem = 536882926 peak = 1090076672 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 286686, 2, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 234, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (194.09, 70.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (277.03, 142.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 225, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (234.28, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 699100 sequences (100000226 bp) in 2.449 CPU sec, 2.643 real sec... [M::mem_process_seqs] Processed 699024 reads in 78.157 CPU sec, 2.510 real sec sorting block 100, #reads = 1084395 mem = 536878872 peak = 1090076672 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286584, 3, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 139, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 289) [M::mem_pestat] mean and std.dev: (128.15, 30.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (277.54, 143.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 176, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (191.54, 62.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 699024 sequences (100000282 bp) in 2.356 CPU sec, 2.498 real sec... [M::mem_process_seqs] Processed 699100 reads in 78.317 CPU sec, 2.518 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 286217, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 154, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (204.45, 107.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (277.57, 143.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 304, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (314.14, 162.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 698960 sequences (100000107 bp) in 2.368 CPU sec, 2.495 real sec... [M::process] read 698382 sequences (100000250 bp) in 1.550 CPU sec, 1.549 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 286531, 2, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 321, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (310.19, 165.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (278.73, 144.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 192, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (211.96, 114.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698960 reads in 79.583 CPU sec, 2.493 real sec sorting block 101, #reads = 1083597 mem = 536881318 peak = 1090076672 [M::process] read 697190 sequences (100000131 bp) in 1.734 CPU sec, 1.786 real sec... [M::mem_process_seqs] Processed 699024 reads in 196.487 CPU sec, 6.231 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 286689, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 197, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (236.71, 148.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 251, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (280.78, 146.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 233, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (263.62, 119.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 102, #reads = 1081401 mem = 536879150 peak = 1106784256 [M::process] read 696864 sequences (100000230 bp) in 2.456 CPU sec, 2.641 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 285545, 6, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 168, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (142.40, 31.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (283.90, 146.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 210, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2248) [M::mem_pestat] mean and std.dev: (431.77, 345.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698382 reads in 158.413 CPU sec, 5.009 real sec [M::mem_process_seqs] Processed 697190 reads in 94.140 CPU sec, 2.966 real sec sorting block 103, #reads = 1081385 mem = 536878532 peak = 1106784256 [M::process] read 696396 sequences (100000167 bp) in 2.331 CPU sec, 2.522 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 286416, 7, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 221, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (271.46, 174.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (285.55, 147.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 255, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 472) [M::mem_pestat] mean and std.dev: (221.73, 63.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696400 sequences (100000208 bp) in 1.920 CPU sec, 1.997 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285788, 2, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 224, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (294.16, 177.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (287.35, 147.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (337.18, 194.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696864 reads in 138.288 CPU sec, 4.381 real sec [M::mem_process_seqs] Processed 696396 reads in 94.075 CPU sec, 2.953 real sec [M::process] read 695778 sequences (100000096 bp) in 1.956 CPU sec, 2.037 real sec... sorting block 104, #reads = 1083495 mem = 536881506 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 285131, 7, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 220, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (216.43, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (288.24, 148.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 174, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (201.21, 104.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695114 sequences (100000217 bp) in 2.467 CPU sec, 2.658 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 284708, 0, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (219.24, 86.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (290.16, 149.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 251, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (309.14, 148.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696400 reads in 139.051 CPU sec, 4.428 real sec [M::mem_process_seqs] Processed 695778 reads in 95.519 CPU sec, 3.013 real sec sorting block 105, #reads = 1081419 mem = 536879058 peak = 1106784256 [M::process] read 694828 sequences (100000127 bp) in 2.525 CPU sec, 2.696 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285261, 10, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 220, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (241.66, 106.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (292.23, 150.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 1419, 2797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7649) [M::mem_pestat] mean and std.dev: (1501.20, 1144.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 240, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (225.83, 106.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 106, #reads = 1080454 mem = 536880641 peak = 1106784256 [M::process] read 694344 sequences (100000130 bp) in 2.218 CPU sec, 2.370 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284725, 4, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 195, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (218.17, 112.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (292.88, 150.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 302, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (303.38, 163.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695114 reads in 142.044 CPU sec, 4.534 real sec [M::mem_process_seqs] Processed 694828 reads in 98.350 CPU sec, 3.097 real sec [M::process] read 694588 sequences (100000029 bp) in 2.671 CPU sec, 2.891 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284646, 4, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 213, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (196.90, 65.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.72, 151.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 252, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (288.39, 150.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 107, #reads = 1079224 mem = 536879375 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284960, 2, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 185, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (205.53, 61.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (292.68, 151.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 222, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 418) [M::mem_pestat] mean and std.dev: (205.25, 55.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694554 sequences (100000020 bp) in 2.458 CPU sec, 2.599 real sec... [M::mem_process_seqs] Processed 694344 reads in 143.501 CPU sec, 4.577 real sec [M::mem_process_seqs] Processed 694588 reads in 98.922 CPU sec, 3.119 real sec sorting block 108, #reads = 1080646 mem = 536877311 peak = 1106784256 [M::process] read 694034 sequences (100000296 bp) in 2.573 CPU sec, 2.736 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284756, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 272, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (266.50, 125.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.94, 151.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 219, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (254.58, 93.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284404, 2, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 151, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (177.40, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (294.56, 151.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 252, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (251.93, 108.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693746 sequences (100000138 bp) in 1.955 CPU sec, 2.187 real sec... [M::mem_process_seqs] Processed 694554 reads in 139.234 CPU sec, 4.422 real sec [M::mem_process_seqs] Processed 694034 reads in 93.855 CPU sec, 2.955 real sec sorting block 109, #reads = 1081045 mem = 536881648 peak = 1106784256 [M::process] read 693416 sequences (100000186 bp) in 2.691 CPU sec, 2.893 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284461, 4, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 243, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (227.12, 112.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (294.68, 150.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 273, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (285.00, 97.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284684, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 138, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (159.85, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (296.67, 152.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 219, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (231.47, 115.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693910 sequences (100000059 bp) in 1.855 CPU sec, 1.962 real sec... [M::mem_process_seqs] Processed 693746 reads in 138.641 CPU sec, 4.427 real sec sorting block 110, #reads = 1080096 mem = 536880086 peak = 1106784256 [M::mem_process_seqs] Processed 693416 reads in 94.680 CPU sec, 2.989 real sec [M::process] read 693776 sequences (100000219 bp) in 2.710 CPU sec, 2.903 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284863, 3, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 266, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (237.92, 101.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (296.08, 153.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 276, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5403) [M::mem_pestat] mean and std.dev: (987.33, 1416.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 111, #reads = 1080710 mem = 536879256 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284508, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 237, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (240.12, 91.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (295.68, 152.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 206, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (203.42, 89.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693276 sequences (100000185 bp) in 2.198 CPU sec, 2.380 real sec... [M::mem_process_seqs] Processed 693910 reads in 139.159 CPU sec, 4.437 real sec [M::mem_process_seqs] Processed 693776 reads in 95.593 CPU sec, 3.011 real sec sorting block 112, #reads = 1081674 mem = 536880751 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283877, 3, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 257, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (241.02, 109.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (298.00, 154.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 240, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (276.15, 132.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693186 sequences (100000262 bp) in 2.685 CPU sec, 2.885 real sec... [M::mem_process_seqs] Processed 693276 reads in 79.210 CPU sec, 2.586 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284520, 5, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 238, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (231.11, 91.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (299.45, 155.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 227, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (235.50, 96.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 113, #reads = 1080813 mem = 536878312 peak = 1106784256 [M::mem_process_seqs] Processed 693186 reads in 78.279 CPU sec, 2.550 real sec [M::process] read 693300 sequences (100000041 bp) in 2.839 CPU sec, 3.282 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284282, 5, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 193, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (251.36, 135.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (300.08, 156.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 275, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (316.19, 186.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693300 reads in 80.177 CPU sec, 2.591 real sec sorting block 114, #reads = 1080385 mem = 536879732 peak = 1106784256 [M::process] read 693192 sequences (100000104 bp) in 3.130 CPU sec, 3.546 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283969, 3, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 160, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (151.88, 46.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (300.36, 157.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 218, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (225.60, 73.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696810 sequences (100000188 bp) in 2.005 CPU sec, 2.201 real sec... [M::mem_process_seqs] Processed 693192 reads in 85.653 CPU sec, 2.736 real sec sorting block 115, #reads = 1077940 mem = 536882241 peak = 1106784256 [M::process] read 700568 sequences (100000142 bp) in 2.384 CPU sec, 2.527 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284787, 3, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 272, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (224.26, 76.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 255, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (285.84, 150.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 240, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (270.39, 119.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 699040 sequences (100000283 bp) in 1.450 CPU sec, 1.448 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 286806, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 208, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (238.62, 132.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (272.88, 142.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 223, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (237.00, 97.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697764 sequences (100000101 bp) in 1.360 CPU sec, 1.354 real sec... [M::mem_process_seqs] Processed 696810 reads in 155.013 CPU sec, 4.869 real sec [M::mem_process_seqs] Processed 700568 reads in 96.436 CPU sec, 3.038 real sec sorting block 116, #reads = 1074211 mem = 536877827 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 286423, 4, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 193, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (252.28, 173.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (277.43, 144.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 347, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (294.16, 108.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697292 sequences (100000168 bp) in 2.916 CPU sec, 3.115 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286135, 5, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 181, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (231.10, 124.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (279.39, 142.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 284, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (309.14, 138.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699040 reads in 131.816 CPU sec, 4.168 real sec [M::mem_process_seqs] Processed 697764 reads in 88.974 CPU sec, 2.802 real sec sorting block 117, #reads = 1085837 mem = 536887216 peak = 1106784256 [M::process] read 697476 sequences (100000039 bp) in 2.119 CPU sec, 2.230 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285982, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 186, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (194.79, 81.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (282.70, 145.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 171, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (207.62, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696708 sequences (100000043 bp) in 2.142 CPU sec, 2.274 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 286504, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 186, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (178.72, 69.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (282.41, 145.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 230, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (213.91, 84.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697292 reads in 133.640 CPU sec, 4.231 real sec [M::mem_process_seqs] Processed 697476 reads in 90.950 CPU sec, 2.856 real sec [M::process] read 696202 sequences (100000279 bp) in 1.805 CPU sec, 1.862 real sec... sorting block 118, #reads = 1085375 mem = 536879386 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285324, 2, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 174, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (241.00, 164.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (283.94, 146.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 241, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (272.28, 142.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696034 sequences (100000137 bp) in 2.276 CPU sec, 2.350 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 286025, 0, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 175, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (39, 379) [M::mem_pestat] mean and std.dev: (191.00, 49.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (286.24, 147.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 267, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (262.05, 90.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696708 reads in 135.372 CPU sec, 4.298 real sec sorting block 119, #reads = 1084589 mem = 536886180 peak = 1106784256 [M::process] read 695934 sequences (100000146 bp) in 1.801 CPU sec, 1.831 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285677, 6, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 133, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (175.58, 101.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (288.39, 149.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 191, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (206.75, 85.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695606 sequences (100000117 bp) in 1.767 CPU sec, 1.770 real sec... [M::mem_process_seqs] Processed 696034 reads in 79.232 CPU sec, 2.664 real sec sorting block 120, #reads = 1083422 mem = 536879574 peak = 1106784256 [M::mem_process_seqs] Processed 696202 reads in 190.134 CPU sec, 6.169 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285208, 12, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (180.71, 63.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (289.38, 149.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (552, 3499, 9244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 26628) [M::mem_pestat] mean and std.dev: (4385.17, 4190.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 35320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 238, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (270.32, 118.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695592 sequences (100000145 bp) in 2.692 CPU sec, 2.761 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 285487, 10, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 184, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (190.93, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (289.93, 149.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (434, 878, 3341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9155) [M::mem_pestat] mean and std.dev: (1978.00, 2010.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 357, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (342.51, 161.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695934 reads in 158.916 CPU sec, 5.032 real sec sorting block 121, #reads = 1084517 mem = 536878518 peak = 1106784256 [M::mem_process_seqs] Processed 695606 reads in 95.375 CPU sec, 2.998 real sec [M::process] read 695550 sequences (100000004 bp) in 2.235 CPU sec, 2.320 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285163, 7, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 234, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (129, 354) [M::mem_pestat] mean and std.dev: (213.42, 41.88) [M::mem_pestat] low and high boundaries for proper pairs: (46, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (289.76, 148.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 286, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (274.69, 86.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 122, #reads = 1082397 mem = 536880689 peak = 1106784256 [M::process] read 694980 sequences (100000146 bp) in 2.324 CPU sec, 2.401 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285421, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 264, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (261.57, 107.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (290.08, 148.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 221, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2187) [M::mem_pestat] mean and std.dev: (456.81, 417.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695592 reads in 141.508 CPU sec, 4.477 real sec [M::mem_process_seqs] Processed 695550 reads in 92.303 CPU sec, 2.903 real sec [M::process] read 695098 sequences (100000157 bp) in 2.234 CPU sec, 2.290 real sec... sorting block 123, #reads = 1081902 mem = 536877742 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285409, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 226, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (212.26, 67.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (292.25, 150.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 273, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (257.77, 108.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694630 sequences (100000088 bp) in 2.534 CPU sec, 2.635 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284996, 5, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 212, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (232.83, 109.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (293.43, 150.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 213, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (209.50, 118.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694980 reads in 135.751 CPU sec, 4.304 real sec [M::mem_process_seqs] Processed 695098 reads in 92.453 CPU sec, 2.908 real sec [M::process] read 693752 sequences (100000278 bp) in 2.229 CPU sec, 2.311 real sec... sorting block 124, #reads = 1083450 mem = 536879019 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284583, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 184, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (202.07, 106.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (293.90, 150.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 220, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (313.83, 173.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1266) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694004 sequences (100000095 bp) in 2.380 CPU sec, 2.475 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284103, 3, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 246, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (232.00, 94.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (295.71, 150.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 199, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (210.32, 81.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694630 reads in 144.046 CPU sec, 4.571 real sec [M::mem_process_seqs] Processed 693752 reads in 96.975 CPU sec, 3.052 real sec sorting block 125, #reads = 1081863 mem = 536880891 peak = 1106784256 [M::process] read 693664 sequences (100000115 bp) in 2.132 CPU sec, 2.202 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283759, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 282, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243) [M::mem_pestat] mean and std.dev: (318.06, 210.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (295.10, 150.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 226, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (216.78, 71.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 126, #reads = 1080140 mem = 536878892 peak = 1106784256 [M::process] read 693502 sequences (100000251 bp) in 2.458 CPU sec, 2.560 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284113, 15, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 219, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (248.69, 113.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (295.59, 150.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 692, 6135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18169) [M::mem_pestat] mean and std.dev: (2286.20, 2710.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24186) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 281, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (254.82, 152.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694004 reads in 143.500 CPU sec, 4.547 real sec [M::mem_process_seqs] Processed 693664 reads in 97.975 CPU sec, 3.081 real sec [M::process] read 693700 sequences (100000179 bp) in 2.051 CPU sec, 2.096 real sec... sorting block 127, #reads = 1080683 mem = 536878213 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284099, 4, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 189, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (219.90, 105.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (294.81, 149.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 220, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (272.84, 158.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694072 sequences (100000183 bp) in 2.675 CPU sec, 2.864 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284069, 5, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 224, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (243.00, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (294.41, 149.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 217, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (270.76, 191.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693502 reads in 142.262 CPU sec, 4.508 real sec [M::mem_process_seqs] Processed 693700 reads in 98.011 CPU sec, 3.085 real sec [M::process] read 693950 sequences (100000154 bp) in 2.233 CPU sec, 2.328 real sec... sorting block 128, #reads = 1080021 mem = 536882215 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284252, 3, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 176, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 383) [M::mem_pestat] mean and std.dev: (197.89, 69.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (294.22, 150.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 287, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (285.93, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693622 sequences (100000214 bp) in 2.733 CPU sec, 2.919 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284783, 0, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 129, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (244.43, 208.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (293.64, 149.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 215, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (250.46, 112.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694072 reads in 145.677 CPU sec, 4.628 real sec [M::mem_process_seqs] Processed 693950 reads in 102.579 CPU sec, 3.236 real sec sorting block 129, #reads = 1080554 mem = 536878179 peak = 1106784256 [M::process] read 693418 sequences (100000051 bp) in 2.454 CPU sec, 2.590 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284548, 1, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 201, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (203.19, 86.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (294.58, 150.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 296, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (284.29, 106.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693490 sequences (100000199 bp) in 2.649 CPU sec, 2.844 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284062, 5, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 305, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (283.33, 96.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (294.39, 150.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 253, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (263.67, 99.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693622 reads in 149.248 CPU sec, 4.751 real sec sorting block 130, #reads = 1076309 mem = 536877801 peak = 1106784256 [M::mem_process_seqs] Processed 693418 reads in 99.066 CPU sec, 3.127 real sec [M::process] read 693710 sequences (100000206 bp) in 2.702 CPU sec, 2.869 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284401, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 323, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (278.26, 124.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (296.26, 151.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 312, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1255) [M::mem_pestat] mean and std.dev: (311.00, 182.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1614) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 131, #reads = 1078726 mem = 536880784 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284297, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 310, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (290.17, 174.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (294.66, 151.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 320, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (300.90, 168.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693426 sequences (100000238 bp) in 2.733 CPU sec, 2.868 real sec... [M::mem_process_seqs] Processed 693490 reads in 143.132 CPU sec, 4.558 real sec [M::mem_process_seqs] Processed 693710 reads in 98.562 CPU sec, 3.109 real sec sorting block 132, #reads = 1078746 mem = 536877393 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283987, 7, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 154, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (194.31, 93.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (296.03, 152.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 201, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (228.60, 120.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693426 reads in 85.281 CPU sec, 2.794 real sec [M::process] read 693384 sequences (100000008 bp) in 3.195 CPU sec, 3.457 real sec... sorting block 133, #reads = 1076653 mem = 536880371 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283774, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 159, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (197.48, 98.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (295.06, 152.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 274, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (281.78, 143.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693384 reads in 98.290 CPU sec, 3.179 real sec [M::process] read 703792 sequences (100000105 bp) in 3.004 CPU sec, 3.210 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 287670, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 174, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (193.65, 66.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (260.77, 135.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (250.42, 146.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703792 reads in 88.589 CPU sec, 2.848 real sec [M::process] read 701728 sequences (100000195 bp) in 2.929 CPU sec, 3.134 real sec... sorting block 134, #reads = 1070008 mem = 536877090 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 286726, 1, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 253, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (221.19, 101.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (267.36, 138.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 267, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (256.04, 105.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 701056 sequences (100000108 bp) in 2.438 CPU sec, 2.607 real sec... [M::mem_process_seqs] Processed 701728 reads in 84.666 CPU sec, 2.725 real sec sorting block 135, #reads = 1083391 mem = 536878424 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 287003, 9, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 155, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (199.14, 110.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (271.04, 140.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 275, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (280.55, 139.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 700126 sequences (100000112 bp) in 2.524 CPU sec, 2.683 real sec... [M::mem_process_seqs] Processed 701056 reads in 82.474 CPU sec, 2.646 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 286473, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (192.77, 67.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.63, 140.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 227, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (240.41, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 699880 sequences (100000197 bp) in 2.521 CPU sec, 2.705 real sec... [M::mem_process_seqs] Processed 700126 reads in 82.803 CPU sec, 2.667 real sec sorting block 136, #reads = 1082656 mem = 536877312 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 287016, 2, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 279, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (264.52, 85.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (274.34, 141.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236) [M::mem_pestat] mean and std.dev: (270.30, 143.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699880 reads in 81.194 CPU sec, 2.609 real sec [M::process] read 701108 sequences (100000216 bp) in 2.602 CPU sec, 2.757 real sec... sorting block 137, #reads = 1082836 mem = 536877665 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 286934, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 203, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (216.64, 89.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (269.82, 139.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 228, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (213.45, 74.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701108 reads in 85.123 CPU sec, 2.732 real sec [M::process] read 700374 sequences (100000173 bp) in 2.722 CPU sec, 2.868 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 287095, 1, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 224, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (250.00, 110.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (273.23, 141.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 202, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (216.54, 115.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 698798 sequences (100000078 bp) in 2.637 CPU sec, 2.789 real sec... [M::mem_process_seqs] Processed 700374 reads in 88.122 CPU sec, 2.832 real sec sorting block 138, #reads = 1080103 mem = 536877339 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 286474, 4, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 191, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (186.00, 84.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (279.80, 145.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (221.25, 86.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698798 reads in 86.520 CPU sec, 2.781 real sec [M::process] read 699910 sequences (100000207 bp) in 3.268 CPU sec, 3.508 real sec... sorting block 139, #reads = 1080590 mem = 536880068 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 286594, 6, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 199, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (177.19, 39.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (273.32, 142.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 289, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (262.13, 108.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697392 sequences (100000183 bp) in 2.509 CPU sec, 2.592 real sec... [M::mem_process_seqs] Processed 699910 reads in 84.682 CPU sec, 2.709 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286101, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 244, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (258.54, 108.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (284.35, 147.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 258, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (263.44, 116.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697520 sequences (100000268 bp) in 2.766 CPU sec, 2.950 real sec... [M::mem_process_seqs] Processed 697392 reads in 89.009 CPU sec, 2.852 real sec sorting block 140, #reads = 1080785 mem = 536880003 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285587, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (219.59, 92.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (283.08, 146.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 212, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (232.74, 88.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696634 sequences (100000226 bp) in 2.468 CPU sec, 2.618 real sec... [M::mem_process_seqs] Processed 697520 reads in 87.773 CPU sec, 2.815 real sec sorting block 141, #reads = 1080199 mem = 536879102 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285800, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (237.12, 117.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (287.87, 149.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 367, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1341) [M::mem_pestat] mean and std.dev: (404.31, 257.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696004 sequences (100000245 bp) in 2.537 CPU sec, 2.690 real sec... [M::mem_process_seqs] Processed 696634 reads in 86.086 CPU sec, 2.765 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285353, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (226.62, 88.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (289.94, 150.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 262, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (298.77, 155.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695428 sequences (100000052 bp) in 2.568 CPU sec, 2.717 real sec... [M::mem_process_seqs] Processed 696004 reads in 87.084 CPU sec, 2.788 real sec sorting block 142, #reads = 1079387 mem = 536883780 peak = 1106784256 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284977, 2, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 191, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1490) [M::mem_pestat] mean and std.dev: (333.28, 306.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (290.00, 149.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 353, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (346.75, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694864 sequences (100000247 bp) in 2.528 CPU sec, 2.677 real sec... [M::mem_process_seqs] Processed 695428 reads in 88.058 CPU sec, 2.823 real sec [M::process] read 694168 sequences (100000091 bp) in 2.507 CPU sec, 2.641 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284389, 8, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 234, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (229.00, 77.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (292.28, 149.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 317, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (291.16, 102.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694612 sequences (100000123 bp) in 1.470 CPU sec, 1.470 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284070, 5, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 258, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (292.95, 189.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (293.11, 149.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 283, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (283.17, 100.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694864 reads in 157.997 CPU sec, 4.964 real sec sorting block 143, #reads = 1078866 mem = 536877069 peak = 1106784256 [M::process] read 694310 sequences (100000241 bp) in 1.472 CPU sec, 1.479 real sec... [M::mem_process_seqs] Processed 694168 reads in 107.987 CPU sec, 3.395 real sec sorting block 144, #reads = 1078268 mem = 536877801 peak = 1117282304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284272, 4, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 198, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (221.03, 114.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (293.03, 150.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 230, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (246.97, 141.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693964 sequences (100000219 bp) in 2.826 CPU sec, 3.038 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284713, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 190, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (212.50, 90.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.72, 151.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 445, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (408.92, 259.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694612 reads in 150.048 CPU sec, 4.747 real sec [M::process] read 693482 sequences (100000109 bp) in 1.850 CPU sec, 1.893 real sec... [M::mem_process_seqs] Processed 694310 reads in 101.987 CPU sec, 3.213 real sec sorting block 145, #reads = 1079516 mem = 536880483 peak = 1117282304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284603, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 175, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (197.00, 69.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (294.09, 151.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 249, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (306.91, 177.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692900 sequences (100000153 bp) in 2.286 CPU sec, 2.440 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284330, 1, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 255, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (239.48, 141.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (295.61, 150.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 193, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (246.76, 142.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693076 sequences (100000042 bp) in 1.480 CPU sec, 1.472 real sec... [M::mem_process_seqs] Processed 693964 reads in 143.838 CPU sec, 4.556 real sec [M::mem_process_seqs] Processed 693482 reads in 96.934 CPU sec, 3.060 real sec sorting block 146, #reads = 1080681 mem = 536882304 peak = 1117282304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284039, 3, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 203, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688) [M::mem_pestat] mean and std.dev: (350.32, 266.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (295.42, 149.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 241, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (241.85, 66.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692958 sequences (100000141 bp) in 2.485 CPU sec, 2.592 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284112, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 205, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (31, 331) [M::mem_pestat] mean and std.dev: (170.00, 37.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (296.02, 150.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 257, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (261.17, 127.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692900 reads in 142.716 CPU sec, 4.522 real sec [M::mem_process_seqs] Processed 693076 reads in 96.784 CPU sec, 3.039 real sec sorting block 147, #reads = 1080200 mem = 536880204 peak = 1117282304 [M::process] read 692238 sequences (100000272 bp) in 2.415 CPU sec, 2.489 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283651, 3, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 208, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (207.80, 81.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (297.18, 151.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 220, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (255.73, 140.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 148, #reads = 1080728 mem = 536880342 peak = 1117282304 [M::process] read 692378 sequences (100000200 bp) in 2.847 CPU sec, 3.004 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283806, 6, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 224, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (313.44, 184.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 272, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (298.52, 151.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 360, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (346.21, 113.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692958 reads in 142.058 CPU sec, 4.508 real sec [M::mem_process_seqs] Processed 692238 reads in 97.345 CPU sec, 3.063 real sec [M::process] read 692536 sequences (100000019 bp) in 3.053 CPU sec, 3.278 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283931, 1, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 229, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (271.56, 153.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (298.88, 152.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 235, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 467) [M::mem_pestat] mean and std.dev: (220.22, 55.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 149, #reads = 1080816 mem = 536878628 peak = 1117282304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283513, 7, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 227, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (208.86, 84.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (299.96, 153.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 192, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (284.40, 195.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692378 reads in 143.732 CPU sec, 4.592 real sec [M::process] read 609986 sequences (88094419 bp) in 2.652 CPU sec, 2.820 real sec... [M::mem_process_seqs] Processed 692536 reads in 99.973 CPU sec, 3.168 real sec sorting block 150, #reads = 1080144 mem = 536877867 peak = 1117282304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 249433, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 293, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (235.68, 109.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 272, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (298.18, 151.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 259, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (244.33, 75.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 609986 reads in 76.196 CPU sec, 2.512 real sec sorting block 151, #reads = 1080272 mem = 536882987 peak = 1183408128 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 163790554 overall: 69889052672 mem 22476.375 user 806.458 sys 802.911 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_87O-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1136_87O-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_87O-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_87O-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_87O-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 802.952 sec; CPU: 23282.837 sec sorting block 152, #reads = 308585 mem = 154356590 peak = 1183408128 uniq header count 1 merging 153 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 8635807683 output reads: 164803803 bam_mem_sort: 153 calls 81.729 user 3.094 sys 85.164 real bam_write: 153 calls 56.213 user 4.084 sys 66.431 real execute: 1 calls 44.596 user 2.326 sys 860.940 real merge_files: 1 calls 44.558 user 2.297 sys 57.511 real parse_chunk: 2437 calls 133.889 user 31.599 sys 169.198 real read_chunk: 6582 calls 77.802 user 50.751 sys 746.109 real sort_block: 1 calls 57.245 user 50.307 sys 717.047 real write_chunk: 4143 calls 44.136 user 1.949 sys 56.408 real overall: 3360833536 mem 2334.169 user 114.447 sys 860.962 real ls: cannot access '*.cram': No such file or directory