.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_cVw-T1-TDNA-1_A23MHWWLT4_1;L004;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/90/86c9203cab60e97029d393989cdd9f/.command.err
Size: 237.0 KB
Task: DAQ:SENTIEON_BWAMEM (659_cVw-T1-TDNA-1_A23MHWWLT4_1;L004;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cVw-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2453.902 CPU sec, 108.266 real sec
[M::process] read 681340 sequences (100000086 bp) in 1.711 CPU sec, 1.720 real sec...
[M::process] read 681072 sequences (100000009 bp) in 2.088 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283931, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (329.53, 157.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680698 sequences (100000296 bp) in 1.970 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284090, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 313, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (332.87, 158.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680822 sequences (100000135 bp) in 2.007 CPU sec, 2.081 real sec...
[M::mem_process_seqs] Processed 681340 reads in 201.530 CPU sec, 6.533 real sec
[M::mem_process_seqs] Processed 681072 reads in 127.459 CPU sec, 4.182 real sec
sorting block 0, #reads = 1086310 mem = 536879131 peak = 581083136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284098, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (333.99, 157.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680544 sequences (100000266 bp) in 3.149 CPU sec, 3.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284279, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (334.27, 157.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680698 reads in 194.287 CPU sec, 6.221 real sec
[M::process] read 680108 sequences (100000203 bp) in 2.530 CPU sec, 2.583 real sec...
[M::mem_process_seqs] Processed 680822 reads in 130.085 CPU sec, 4.147 real sec
sorting block 1, #reads = 1085098 mem = 536879937 peak = 705339392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283791, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (335.24, 157.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679626 sequences (100000255 bp) in 2.800 CPU sec, 3.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283526, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 320, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (337.87, 158.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679408 sequences (100000281 bp) in 1.987 CPU sec, 2.011 real sec...
[M::mem_process_seqs] Processed 680544 reads in 196.502 CPU sec, 6.290 real sec
[M::mem_process_seqs] Processed 680108 reads in 127.813 CPU sec, 4.084 real sec
sorting block 2, #reads = 1084256 mem = 536878908 peak = 754249728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283394, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (341.69, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679256 sequences (100000175 bp) in 3.206 CPU sec, 3.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283462, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (342.37, 160.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679626 reads in 192.867 CPU sec, 6.177 real sec
[M::mem_process_seqs] Processed 679408 reads in 128.170 CPU sec, 4.087 real sec
[M::process] read 678638 sequences (100000165 bp) in 2.807 CPU sec, 2.894 real sec...
sorting block 3, #reads = 1083620 mem = 536880132 peak = 764170240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283611, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (345.36, 161.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678938 sequences (100000295 bp) in 3.052 CPU sec, 3.219 real sec...
sorting block 4, #reads = 1083652 mem = 536879208 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282918, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (348.53, 162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679256 reads in 191.655 CPU sec, 6.207 real sec
[M::mem_process_seqs] Processed 678638 reads in 127.586 CPU sec, 4.105 real sec
[M::process] read 678378 sequences (100000168 bp) in 3.020 CPU sec, 3.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282377, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (349.29, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1082924 mem = 536881379 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283519, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.33, 163.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678052 sequences (100000180 bp) in 3.557 CPU sec, 3.830 real sec...
[M::mem_process_seqs] Processed 678938 reads in 188.613 CPU sec, 6.121 real sec
[M::mem_process_seqs] Processed 678378 reads in 124.691 CPU sec, 4.006 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282941, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (353.39, 164.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678094 sequences (100000220 bp) in 3.260 CPU sec, 3.821 real sec...
[M::mem_process_seqs] Processed 678052 reads in 103.502 CPU sec, 3.481 real sec
sorting block 6, #reads = 1082960 mem = 536880904 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282683, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (353.85, 165.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678094 reads in 105.095 CPU sec, 3.493 real sec
[M::process] read 678106 sequences (100000067 bp) in 3.575 CPU sec, 3.932 real sec...
sorting block 7, #reads = 1084007 mem = 536880789 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283112, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (352.56, 164.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678106 reads in 104.947 CPU sec, 3.507 real sec
[M::process] read 677940 sequences (100000183 bp) in 3.439 CPU sec, 3.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282729, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.67, 163.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 8, #reads = 1082558 mem = 536878538 peak = 813830144
[M::mem_process_seqs] Processed 677940 reads in 103.272 CPU sec, 3.447 real sec
[M::process] read 677904 sequences (100000263 bp) in 3.258 CPU sec, 3.616 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282429, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.41, 163.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677904 reads in 102.777 CPU sec, 3.419 real sec
[M::process] read 678082 sequences (100000139 bp) in 3.138 CPU sec, 3.518 real sec...
sorting block 9, #reads = 1083044 mem = 536880936 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282070, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (350.28, 162.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678082 reads in 103.982 CPU sec, 3.481 real sec
[M::process] read 677872 sequences (100000274 bp) in 3.663 CPU sec, 3.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282495, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.53, 162.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 10, #reads = 1082230 mem = 536878633 peak = 813830144
[M::mem_process_seqs] Processed 677872 reads in 98.345 CPU sec, 3.237 real sec
[M::process] read 677940 sequences (100000228 bp) in 3.409 CPU sec, 3.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282746, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (348.75, 161.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677940 reads in 107.297 CPU sec, 3.531 real sec
[M::process] read 678144 sequences (100000151 bp) in 3.599 CPU sec, 3.960 real sec...
sorting block 11, #reads = 1082536 mem = 536876844 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282632, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (347.92, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678144 reads in 111.026 CPU sec, 3.638 real sec
[M::process] read 678106 sequences (100000027 bp) in 3.659 CPU sec, 4.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282293, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (347.84, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678106 reads in 110.651 CPU sec, 3.676 real sec
[M::process] read 678364 sequences (100000221 bp) in 3.556 CPU sec, 3.863 real sec...
sorting block 12, #reads = 1083059 mem = 536884422 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282561, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (346.86, 161.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678364 reads in 112.181 CPU sec, 3.709 real sec
[M::process] read 678032 sequences (100000260 bp) in 3.800 CPU sec, 4.219 real sec...
sorting block 13, #reads = 1082857 mem = 536880527 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282449, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (350.02, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678032 reads in 110.924 CPU sec, 3.658 real sec
[M::process] read 677808 sequences (100000245 bp) in 3.655 CPU sec, 4.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282236, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (349.98, 162.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677808 reads in 110.022 CPU sec, 3.616 real sec
[M::process] read 677770 sequences (100000209 bp) in 3.754 CPU sec, 4.100 real sec...
sorting block 14, #reads = 1082961 mem = 536878143 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282261, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (350.24, 163.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677770 reads in 110.347 CPU sec, 3.620 real sec
[M::process] read 677682 sequences (100000146 bp) in 3.544 CPU sec, 3.942 real sec...
sorting block 15, #reads = 1082718 mem = 536877291 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282156, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.45, 163.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677682 reads in 108.667 CPU sec, 3.565 real sec
[M::process] read 677688 sequences (100000091 bp) in 3.845 CPU sec, 4.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281957, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.81, 162.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677688 reads in 108.741 CPU sec, 3.539 real sec
sorting block 16, #reads = 1082123 mem = 536880979 peak = 813830144
[M::process] read 677622 sequences (100000141 bp) in 3.708 CPU sec, 4.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282476, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.78, 162.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677520 sequences (100000002 bp) in 3.387 CPU sec, 3.639 real sec...
[M::mem_process_seqs] Processed 677622 reads in 113.792 CPU sec, 3.709 real sec
sorting block 17, #reads = 1082137 mem = 536880692 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282727, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.80, 161.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677520 reads in 110.693 CPU sec, 3.606 real sec
[M::process] read 677306 sequences (100000154 bp) in 3.910 CPU sec, 4.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282732, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.44, 161.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677038 sequences (100000042 bp) in 2.993 CPU sec, 3.133 real sec...
[M::mem_process_seqs] Processed 677306 reads in 105.019 CPU sec, 3.403 real sec
sorting block 18, #reads = 1082282 mem = 536878889 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282531, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (352.81, 161.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677038 reads in 103.235 CPU sec, 3.343 real sec
[M::process] read 677154 sequences (100000222 bp) in 3.984 CPU sec, 4.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282056, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (352.54, 161.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1083006 mem = 536880469 peak = 813830144
[M::mem_process_seqs] Processed 677154 reads in 102.271 CPU sec, 3.316 real sec
[M::process] read 676928 sequences (100000013 bp) in 3.424 CPU sec, 3.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282457, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (355.42, 162.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676806 sequences (100000023 bp) in 2.630 CPU sec, 2.850 real sec...
[M::mem_process_seqs] Processed 676928 reads in 98.564 CPU sec, 3.189 real sec
sorting block 20, #reads = 1081631 mem = 536878220 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281522, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (357.11, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676806 reads in 100.714 CPU sec, 3.259 real sec
[M::process] read 676628 sequences (100000063 bp) in 3.407 CPU sec, 3.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282989, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 343, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (359.42, 165.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1082649 mem = 536879372 peak = 813830144
[M::mem_process_seqs] Processed 676628 reads in 98.967 CPU sec, 3.201 real sec
[M::process] read 676566 sequences (100000088 bp) in 3.037 CPU sec, 3.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281773, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (359.47, 165.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676566 reads in 99.960 CPU sec, 3.236 real sec
[M::process] read 676414 sequences (100000281 bp) in 3.153 CPU sec, 3.361 real sec...
sorting block 22, #reads = 1082119 mem = 536879340 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282656, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 345, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (360.80, 165.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676414 reads in 97.783 CPU sec, 3.172 real sec
[M::process] read 676272 sequences (100000060 bp) in 3.465 CPU sec, 3.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282344, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (359.69, 165.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676272 reads in 102.183 CPU sec, 3.302 real sec
[M::process] read 681338 sequences (100000275 bp) in 3.261 CPU sec, 3.459 real sec...
sorting block 23, #reads = 1082423 mem = 536880456 peak = 813830144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283237, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 313, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (333.70, 159.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681002 sequences (100000058 bp) in 3.181 CPU sec, 3.341 real sec...
[M::mem_process_seqs] Processed 681338 reads in 104.717 CPU sec, 3.398 real sec
sorting block 24, #reads = 1083160 mem = 536878923 peak = 836063232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282783, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 318, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (337.10, 160.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681002 reads in 103.900 CPU sec, 3.388 real sec
[M::process] read 680566 sequences (100000017 bp) in 3.316 CPU sec, 3.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282916, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (340.62, 162.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680566 reads in 102.654 CPU sec, 3.322 real sec
[M::process] read 680626 sequences (100000166 bp) in 3.498 CPU sec, 3.712 real sec...
sorting block 25, #reads = 1086200 mem = 536878958 peak = 836063232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283108, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (339.89, 161.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680532 sequences (100000039 bp) in 2.971 CPU sec, 3.169 real sec...
[M::mem_process_seqs] Processed 680626 reads in 101.439 CPU sec, 3.286 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283775, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (340.20, 160.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680532 reads in 98.063 CPU sec, 3.179 real sec
sorting block 26, #reads = 1085526 mem = 536880035 peak = 852406272
[M::process] read 679944 sequences (100000251 bp) in 3.733 CPU sec, 3.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283047, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (342.18, 161.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679944 reads in 103.789 CPU sec, 3.370 real sec
[M::process] read 679960 sequences (100000162 bp) in 3.317 CPU sec, 3.597 real sec...
sorting block 27, #reads = 1085679 mem = 536880008 peak = 852406272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282925, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (342.59, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679704 sequences (100000135 bp) in 3.139 CPU sec, 3.287 real sec...
[M::mem_process_seqs] Processed 679960 reads in 101.596 CPU sec, 3.302 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283335, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (345.77, 163.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679704 reads in 101.778 CPU sec, 3.286 real sec
sorting block 28, #reads = 1084640 mem = 536881976 peak = 852406272
[M::process] read 679470 sequences (100000113 bp) in 3.456 CPU sec, 3.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282590, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (345.38, 161.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679470 reads in 101.266 CPU sec, 3.280 real sec
[M::process] read 678884 sequences (100000251 bp) in 3.197 CPU sec, 3.338 real sec...
sorting block 29, #reads = 1085019 mem = 536878752 peak = 852406272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282955, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (350.16, 163.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678884 reads in 102.225 CPU sec, 3.319 real sec
[M::process] read 678720 sequences (100000166 bp) in 3.579 CPU sec, 3.810 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282930, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (349.50, 162.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678720 reads in 99.549 CPU sec, 3.227 real sec
[M::process] read 678806 sequences (100000298 bp) in 3.361 CPU sec, 3.611 real sec...
sorting block 30, #reads = 1084570 mem = 536880375 peak = 860389376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282748, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (352.25, 164.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678330 sequences (100000039 bp) in 2.895 CPU sec, 3.066 real sec...
[M::mem_process_seqs] Processed 678806 reads in 98.728 CPU sec, 3.176 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282356, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (356.09, 166.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1085021 mem = 536882165 peak = 892334080
[M::mem_process_seqs] Processed 678330 reads in 99.699 CPU sec, 3.237 real sec
[M::process] read 678324 sequences (100000163 bp) in 3.538 CPU sec, 3.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283033, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (353.89, 164.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678400 sequences (100000060 bp) in 2.857 CPU sec, 2.983 real sec...
[M::mem_process_seqs] Processed 678324 reads in 96.842 CPU sec, 3.133 real sec
sorting block 32, #reads = 1083941 mem = 536882600 peak = 892334080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282976, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (354.09, 165.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678400 reads in 100.480 CPU sec, 3.261 real sec
[M::process] read 678014 sequences (100000214 bp) in 3.919 CPU sec, 4.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282460, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (356.17, 166.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678014 reads in 95.923 CPU sec, 3.107 real sec
sorting block 33, #reads = 1084010 mem = 536877421 peak = 892334080
[M::process] read 677972 sequences (100000181 bp) in 3.440 CPU sec, 3.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282746, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 338, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (355.37, 165.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677972 reads in 101.049 CPU sec, 3.277 real sec
[M::process] read 678096 sequences (100000164 bp) in 3.364 CPU sec, 3.530 real sec...
sorting block 34, #reads = 1083944 mem = 536882353 peak = 892334080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282675, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (354.10, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678122 sequences (100000275 bp) in 2.984 CPU sec, 3.145 real sec...
[M::mem_process_seqs] Processed 678096 reads in 102.747 CPU sec, 3.315 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282610, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (353.73, 165.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678182 sequences (100000256 bp) in 3.181 CPU sec, 3.357 real sec...
[M::mem_process_seqs] Processed 678122 reads in 102.215 CPU sec, 3.304 real sec
sorting block 35, #reads = 1082696 mem = 536878321 peak = 892334080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282426, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (353.21, 165.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678182 reads in 101.378 CPU sec, 3.290 real sec
[M::process] read 678038 sequences (100000184 bp) in 3.403 CPU sec, 3.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282503, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (349.87, 162.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1083088 mem = 536879213 peak = 892334080
[M::mem_process_seqs] Processed 678038 reads in 100.340 CPU sec, 3.239 real sec
[M::process] read 678554 sequences (100000277 bp) in 3.525 CPU sec, 3.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282605, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (344.66, 160.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678554 reads in 99.818 CPU sec, 3.238 real sec
[M::process] read 678536 sequences (100000223 bp) in 3.079 CPU sec, 3.277 real sec...
sorting block 37, #reads = 1083104 mem = 536878471 peak = 892334080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282772, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (344.25, 160.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678536 reads in 99.368 CPU sec, 3.214 real sec
[M::process] read 678704 sequences (100000296 bp) in 3.194 CPU sec, 3.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282880, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (344.33, 160.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678414 sequences (100000071 bp) in 3.131 CPU sec, 3.282 real sec...
[M::mem_process_seqs] Processed 678704 reads in 102.117 CPU sec, 3.287 real sec
sorting block 38, #reads = 1084033 mem = 536877787 peak = 892334080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282325, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (345.02, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678414 reads in 101.459 CPU sec, 3.287 real sec
[M::process] read 678524 sequences (100000226 bp) in 3.336 CPU sec, 3.507 real sec...
sorting block 39, #reads = 1083089 mem = 536882465 peak = 892334080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282759, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (345.74, 161.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678144 sequences (100000037 bp) in 3.018 CPU sec, 3.188 real sec...
[M::mem_process_seqs] Processed 678524 reads in 99.324 CPU sec, 3.231 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282511, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (346.75, 161.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678144 reads in 100.097 CPU sec, 3.243 real sec
sorting block 40, #reads = 1084301 mem = 536876782 peak = 892334080
[M::process] read 678106 sequences (100000301 bp) in 3.965 CPU sec, 4.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282179, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (348.01, 161.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678106 reads in 98.312 CPU sec, 3.198 real sec
[M::process] read 678038 sequences (100000168 bp) in 2.978 CPU sec, 3.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282489, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (346.18, 160.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1082557 mem = 536879827 peak = 892334080
[M::mem_process_seqs] Processed 678038 reads in 97.650 CPU sec, 3.163 real sec
[M::process] read 678158 sequences (100000021 bp) in 3.754 CPU sec, 4.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282778, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (348.95, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677590 sequences (100000278 bp) in 2.527 CPU sec, 2.678 real sec...
[M::mem_process_seqs] Processed 678158 reads in 96.428 CPU sec, 3.103 real sec
sorting block 42, #reads = 1082151 mem = 536878735 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282533, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (351.59, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677590 reads in 98.389 CPU sec, 3.180 real sec
[M::process] read 677742 sequences (100000097 bp) in 3.569 CPU sec, 3.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282159, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (353.44, 164.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1083126 mem = 536878697 peak = 902565888
[M::mem_process_seqs] Processed 677742 reads in 95.957 CPU sec, 3.118 real sec
[M::process] read 677422 sequences (100000207 bp) in 3.311 CPU sec, 3.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282551, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (353.29, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677422 reads in 95.063 CPU sec, 3.068 real sec
sorting block 44, #reads = 1081724 mem = 536876923 peak = 902565888
[M::process] read 677374 sequences (100000169 bp) in 3.975 CPU sec, 4.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282239, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (354.29, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677190 sequences (100000049 bp) in 2.667 CPU sec, 2.805 real sec...
[M::mem_process_seqs] Processed 677374 reads in 94.783 CPU sec, 3.057 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282586, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (355.09, 164.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677190 reads in 95.724 CPU sec, 3.089 real sec
[M::process] read 677450 sequences (100000261 bp) in 3.556 CPU sec, 3.744 real sec...
sorting block 45, #reads = 1082924 mem = 536879227 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282497, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.93, 164.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677450 reads in 92.383 CPU sec, 2.991 real sec
[M::process] read 677122 sequences (100000251 bp) in 3.282 CPU sec, 3.506 real sec...
sorting block 46, #reads = 1082903 mem = 536877824 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282244, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 340, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (357.74, 167.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677122 reads in 95.352 CPU sec, 3.081 real sec
[M::process] read 677590 sequences (100000092 bp) in 3.362 CPU sec, 3.534 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283316, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 340, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (358.59, 168.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677590 reads in 94.662 CPU sec, 3.063 real sec
[M::process] read 677274 sequences (100000095 bp) in 3.654 CPU sec, 3.831 real sec...
sorting block 47, #reads = 1081101 mem = 536878236 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282229, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 344, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (361.48, 170.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681292 sequences (100000251 bp) in 2.869 CPU sec, 2.984 real sec...
[M::mem_process_seqs] Processed 677274 reads in 95.685 CPU sec, 3.082 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283911, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (335.11, 159.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1081331 mem = 536875988 peak = 902565888
[M::mem_process_seqs] Processed 681292 reads in 95.261 CPU sec, 3.074 real sec
[M::process] read 681742 sequences (100000051 bp) in 3.387 CPU sec, 3.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283780, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 312, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (330.95, 156.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681742 reads in 93.829 CPU sec, 3.040 real sec
[M::process] read 681346 sequences (100000022 bp) in 3.366 CPU sec, 3.540 real sec...
sorting block 49, #reads = 1085599 mem = 536879901 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283163, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (333.71, 157.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681346 reads in 98.390 CPU sec, 3.179 real sec
[M::process] read 681620 sequences (100000157 bp) in 3.107 CPU sec, 3.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283771, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (333.06, 156.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681620 reads in 98.924 CPU sec, 3.199 real sec
sorting block 50, #reads = 1084870 mem = 536878376 peak = 902565888
[M::process] read 681154 sequences (100000218 bp) in 3.353 CPU sec, 3.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283560, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (333.02, 155.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681154 reads in 98.814 CPU sec, 3.184 real sec
[M::process] read 680636 sequences (100000192 bp) in 3.228 CPU sec, 3.411 real sec...
sorting block 51, #reads = 1085471 mem = 536876268 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283411, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (334.69, 155.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680636 reads in 99.311 CPU sec, 3.216 real sec
[M::process] read 680204 sequences (100000251 bp) in 3.474 CPU sec, 3.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283437, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (336.68, 155.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680204 reads in 99.709 CPU sec, 3.231 real sec
[M::process] read 680110 sequences (100000135 bp) in 3.565 CPU sec, 3.759 real sec...
sorting block 52, #reads = 1084192 mem = 536881077 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283445, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (338.93, 157.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680110 reads in 100.757 CPU sec, 3.251 real sec
[M::process] read 679764 sequences (100000141 bp) in 3.425 CPU sec, 3.652 real sec...
sorting block 53, #reads = 1084672 mem = 536878645 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283456, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (340.28, 157.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679764 reads in 96.154 CPU sec, 3.121 real sec
[M::process] read 679446 sequences (100000061 bp) in 3.148 CPU sec, 3.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282824, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (342.05, 157.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679196 sequences (100000012 bp) in 2.914 CPU sec, 3.073 real sec...
[M::process] read 678948 sequences (100000075 bp) in 2.158 CPU sec, 2.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282870, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (343.53, 157.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679196 reads in 98.058 CPU sec, 3.077 real sec
sorting block 54, #reads = 1083821 mem = 536878679 peak = 902565888
[M::process] read 678568 sequences (100000281 bp) in 3.083 CPU sec, 3.226 real sec...
[M::mem_process_seqs] Processed 679446 reads in 275.658 CPU sec, 8.764 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282994, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 327, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (342.79, 157.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282665, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (347.12, 159.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678948 reads in 199.950 CPU sec, 6.354 real sec
sorting block 55, #reads = 1083888 mem = 536879180 peak = 902565888
[M::process] read 678238 sequences (100000206 bp) in 3.583 CPU sec, 3.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282882, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (349.15, 160.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678192 sequences (100000202 bp) in 2.626 CPU sec, 2.754 real sec...
[M::mem_process_seqs] Processed 678238 reads in 102.252 CPU sec, 3.294 real sec
sorting block 56, #reads = 1083925 mem = 536879404 peak = 902565888
[M::mem_process_seqs] Processed 678568 reads in 298.535 CPU sec, 9.522 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282505, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (346.56, 158.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678628 sequences (100000092 bp) in 3.244 CPU sec, 3.402 real sec...
[M::mem_process_seqs] Processed 678192 reads in 103.984 CPU sec, 3.375 real sec
sorting block 57, #reads = 1081975 mem = 536879173 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282483, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (347.77, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678220 sequences (100000185 bp) in 3.108 CPU sec, 3.307 real sec...
[M::mem_process_seqs] Processed 678628 reads in 102.541 CPU sec, 3.377 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282810, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (349.54, 160.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1082720 mem = 536877834 peak = 902565888
[M::mem_process_seqs] Processed 678220 reads in 103.275 CPU sec, 3.354 real sec
[M::process] read 677814 sequences (100000224 bp) in 3.818 CPU sec, 4.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282555, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (351.57, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677814 reads in 101.448 CPU sec, 3.308 real sec
[M::process] read 677934 sequences (100000273 bp) in 3.314 CPU sec, 3.624 real sec...
sorting block 59, #reads = 1082684 mem = 536879732 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282958, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.08, 160.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677934 reads in 101.972 CPU sec, 3.284 real sec
[M::process] read 678506 sequences (100000269 bp) in 3.134 CPU sec, 3.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282560, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (345.49, 160.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1082363 mem = 536883169 peak = 902565888
[M::process] read 678376 sequences (100000003 bp) in 2.995 CPU sec, 3.171 real sec...
[M::mem_process_seqs] Processed 678506 reads in 100.777 CPU sec, 3.252 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283109, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (344.86, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678204 sequences (100000031 bp) in 3.154 CPU sec, 3.312 real sec...
[M::mem_process_seqs] Processed 678376 reads in 100.437 CPU sec, 3.264 real sec
sorting block 61, #reads = 1082904 mem = 536878584 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282853, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (347.05, 160.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678204 reads in 102.446 CPU sec, 3.321 real sec
[M::process] read 678238 sequences (100000214 bp) in 3.471 CPU sec, 3.701 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282748, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (345.08, 159.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678238 reads in 102.134 CPU sec, 3.296 real sec
[M::process] read 678472 sequences (100000158 bp) in 3.136 CPU sec, 3.313 real sec...
sorting block 62, #reads = 1082216 mem = 536876447 peak = 902565888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282418, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (344.15, 159.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678472 reads in 101.940 CPU sec, 3.305 real sec
[M::process] read 678432 sequences (100000159 bp) in 3.164 CPU sec, 3.492 real sec...
sorting block 63, #reads = 1083412 mem = 536878169 peak = 909373440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282251, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (345.76, 160.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678432 reads in 101.229 CPU sec, 3.273 real sec
[M::process] read 678386 sequences (100000051 bp) in 3.133 CPU sec, 3.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282757, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (345.17, 159.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678386 reads in 102.175 CPU sec, 3.321 real sec
[M::process] read 677994 sequences (100000078 bp) in 3.432 CPU sec, 3.648 real sec...
sorting block 64, #reads = 1082770 mem = 536880006 peak = 909373440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282481, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (348.69, 160.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678138 sequences (100000078 bp) in 3.078 CPU sec, 3.285 real sec...
[M::mem_process_seqs] Processed 677994 reads in 102.533 CPU sec, 3.409 real sec
sorting block 65, #reads = 1081683 mem = 536879854 peak = 909373440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282858, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (346.89, 160.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678008 sequences (100000131 bp) in 2.960 CPU sec, 3.130 real sec...
[M::mem_process_seqs] Processed 678138 reads in 105.414 CPU sec, 3.415 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282465, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (344.31, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677812 sequences (100000179 bp) in 3.459 CPU sec, 3.689 real sec...
[M::mem_process_seqs] Processed 678008 reads in 105.039 CPU sec, 3.392 real sec
sorting block 66, #reads = 1080815 mem = 536881305 peak = 909373440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283033, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (346.36, 159.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677812 reads in 102.832 CPU sec, 3.322 real sec
[M::process] read 677856 sequences (100000004 bp) in 3.436 CPU sec, 3.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282980, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.04, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677856 reads in 102.322 CPU sec, 3.302 real sec
[M::process] read 677660 sequences (100000227 bp) in 3.215 CPU sec, 3.422 real sec...
sorting block 67, #reads = 1082166 mem = 536877799 peak = 909373440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282376, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.58, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677660 reads in 101.954 CPU sec, 3.316 real sec
[M::process] read 677970 sequences (100000264 bp) in 3.601 CPU sec, 3.839 real sec...
sorting block 68, #reads = 1082085 mem = 536880160 peak = 915484672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282786, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.04, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677970 reads in 101.854 CPU sec, 3.299 real sec
[M::process] read 677682 sequences (100000204 bp) in 3.065 CPU sec, 3.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282038, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (354.04, 164.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677682 reads in 103.792 CPU sec, 3.358 real sec
[M::process] read 677522 sequences (100000128 bp) in 3.503 CPU sec, 3.721 real sec...
sorting block 69, #reads = 1080735 mem = 536880853 peak = 915484672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282598, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (352.32, 164.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677522 reads in 100.134 CPU sec, 3.247 real sec
[M::process] read 677822 sequences (100000196 bp) in 3.191 CPU sec, 3.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282965, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (352.90, 165.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 70, #reads = 1081509 mem = 536880691 peak = 915484672
[M::mem_process_seqs] Processed 677822 reads in 101.393 CPU sec, 3.270 real sec
[M::process] read 677558 sequences (100000081 bp) in 3.222 CPU sec, 3.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282870, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (355.01, 166.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677558 reads in 100.883 CPU sec, 3.260 real sec
[M::process] read 677888 sequences (100000054 bp) in 3.796 CPU sec, 4.077 real sec...
sorting block 71, #reads = 1082050 mem = 536880034 peak = 915484672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282717, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (353.07, 166.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677888 reads in 99.282 CPU sec, 3.212 real sec
[M::process] read 679588 sequences (100000246 bp) in 3.167 CPU sec, 3.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283005, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (341.60, 160.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679588 reads in 108.310 CPU sec, 3.508 real sec
sorting block 72, #reads = 1080985 mem = 536879847 peak = 915484672
[M::process] read 681208 sequences (100000265 bp) in 3.896 CPU sec, 4.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283221, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (332.29, 156.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680988 sequences (100000021 bp) in 2.992 CPU sec, 3.206 real sec...
[M::mem_process_seqs] Processed 681208 reads in 100.862 CPU sec, 3.267 real sec
sorting block 73, #reads = 1081767 mem = 536878781 peak = 928366592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283399, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (334.61, 157.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680988 reads in 100.875 CPU sec, 3.279 real sec
[M::process] read 680588 sequences (100000194 bp) in 3.455 CPU sec, 3.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283635, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (337.25, 158.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680588 reads in 101.304 CPU sec, 3.279 real sec
[M::process] read 680624 sequences (100000042 bp) in 3.195 CPU sec, 3.468 real sec...
sorting block 74, #reads = 1085539 mem = 536881335 peak = 928366592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282620, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (336.43, 157.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680624 reads in 103.243 CPU sec, 3.353 real sec
[M::process] read 680428 sequences (100000139 bp) in 3.718 CPU sec, 3.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283009, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (338.25, 158.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 75, #reads = 1084965 mem = 536888958 peak = 928366592
[M::mem_process_seqs] Processed 680428 reads in 111.134 CPU sec, 3.621 real sec
[M::process] read 680058 sequences (100000203 bp) in 3.901 CPU sec, 4.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283068, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (340.15, 159.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680058 reads in 106.502 CPU sec, 3.467 real sec
[M::process] read 680062 sequences (100000245 bp) in 3.359 CPU sec, 3.644 real sec...
sorting block 76, #reads = 1084820 mem = 536880292 peak = 928366592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282607, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (339.76, 158.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680062 reads in 110.447 CPU sec, 3.599 real sec
[M::process] read 679768 sequences (100000169 bp) in 3.552 CPU sec, 3.829 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282798, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (341.27, 158.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679768 reads in 107.774 CPU sec, 3.521 real sec
[M::process] read 679472 sequences (100000206 bp) in 3.655 CPU sec, 3.917 real sec...
sorting block 77, #reads = 1084777 mem = 536878048 peak = 932847616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282579, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (343.03, 158.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679472 reads in 106.744 CPU sec, 3.498 real sec
[M::process] read 678984 sequences (100000088 bp) in 3.508 CPU sec, 3.848 real sec...
sorting block 78, #reads = 1084035 mem = 536881355 peak = 935378944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282264, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (345.65, 160.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678984 reads in 106.812 CPU sec, 3.490 real sec
[M::process] read 678814 sequences (100000300 bp) in 3.704 CPU sec, 4.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282624, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (346.82, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678814 reads in 102.992 CPU sec, 3.344 real sec
sorting block 79, #reads = 1082709 mem = 536879631 peak = 935378944
[M::process] read 678828 sequences (100000087 bp) in 3.652 CPU sec, 3.937 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282594, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (346.82, 159.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678828 reads in 107.208 CPU sec, 3.490 real sec
[M::process] read 678606 sequences (100000202 bp) in 3.283 CPU sec, 3.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282682, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (346.36, 158.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1083965 mem = 536879714 peak = 945922048
[M::mem_process_seqs] Processed 678606 reads in 103.049 CPU sec, 3.356 real sec
[M::process] read 678454 sequences (100000127 bp) in 3.437 CPU sec, 3.739 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282462, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (348.17, 159.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678454 reads in 105.856 CPU sec, 3.448 real sec
[M::process] read 678428 sequences (100000213 bp) in 3.885 CPU sec, 4.178 real sec...
sorting block 81, #reads = 1084004 mem = 536878811 peak = 945922048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282261, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (347.91, 159.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678428 reads in 106.721 CPU sec, 3.458 real sec
[M::process] read 678342 sequences (100000239 bp) in 3.860 CPU sec, 4.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282276, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (347.26, 158.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 82, #reads = 1083349 mem = 536878128 peak = 945922048
[M::mem_process_seqs] Processed 678342 reads in 104.926 CPU sec, 3.405 real sec
[M::process] read 678436 sequences (100000176 bp) in 3.622 CPU sec, 3.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282829, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (347.52, 159.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678436 reads in 107.062 CPU sec, 3.469 real sec
[M::process] read 678524 sequences (100000261 bp) in 3.342 CPU sec, 3.592 real sec...
sorting block 83, #reads = 1082774 mem = 536879656 peak = 945922048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282358, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (349.53, 161.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678524 reads in 107.451 CPU sec, 3.474 real sec
[M::process] read 678408 sequences (100000010 bp) in 3.495 CPU sec, 3.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282062, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (349.18, 162.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680410 sequences (100000178 bp) in 3.280 CPU sec, 3.468 real sec...
[M::mem_process_seqs] Processed 678408 reads in 107.236 CPU sec, 3.472 real sec
sorting block 84, #reads = 1082564 mem = 536878096 peak = 945922048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282973, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 313, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (335.40, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680410 reads in 105.073 CPU sec, 3.401 real sec
[M::process] read 679116 sequences (100000052 bp) in 3.725 CPU sec, 3.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282617, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 320, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (339.12, 157.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 85, #reads = 1082857 mem = 536879543 peak = 966742016
[M::mem_process_seqs] Processed 679116 reads in 109.952 CPU sec, 3.572 real sec
[M::process] read 678810 sequences (100000249 bp) in 3.831 CPU sec, 4.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283102, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (341.60, 157.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678810 reads in 109.947 CPU sec, 3.570 real sec
sorting block 86, #reads = 1082390 mem = 536879469 peak = 966742016
[M::process] read 678962 sequences (100000270 bp) in 3.983 CPU sec, 4.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282721, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 322, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (338.94, 156.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678962 reads in 108.998 CPU sec, 3.524 real sec
[M::process] read 678720 sequences (100000044 bp) in 3.750 CPU sec, 3.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282455, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (340.96, 156.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 87, #reads = 1081288 mem = 536879850 peak = 966742016
[M::mem_process_seqs] Processed 678720 reads in 109.332 CPU sec, 3.537 real sec
[M::process] read 678586 sequences (100000210 bp) in 3.705 CPU sec, 4.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282152, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 323, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (339.66, 155.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678384 sequences (100000104 bp) in 3.279 CPU sec, 3.455 real sec...
[M::mem_process_seqs] Processed 678586 reads in 110.392 CPU sec, 3.579 real sec
sorting block 88, #reads = 1081620 mem = 536878936 peak = 966742016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282111, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 323, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (339.56, 155.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678384 reads in 109.731 CPU sec, 3.575 real sec
[M::process] read 678500 sequences (100000033 bp) in 3.514 CPU sec, 3.768 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282230, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (340.64, 156.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678500 reads in 108.576 CPU sec, 3.538 real sec
[M::process] read 678400 sequences (100000213 bp) in 3.445 CPU sec, 3.700 real sec...
sorting block 89, #reads = 1082476 mem = 536879199 peak = 966742016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282705, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (339.39, 155.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678400 reads in 110.777 CPU sec, 3.613 real sec
[M::process] read 678354 sequences (100000276 bp) in 3.451 CPU sec, 3.765 real sec...
sorting block 90, #reads = 1081971 mem = 536878937 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282419, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 322, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (338.97, 154.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678354 reads in 105.042 CPU sec, 3.422 real sec
[M::process] read 678272 sequences (100000216 bp) in 3.460 CPU sec, 3.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282303, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (337.27, 153.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678094 sequences (100000239 bp) in 3.127 CPU sec, 3.339 real sec...
[M::mem_process_seqs] Processed 678272 reads in 105.887 CPU sec, 3.427 real sec
sorting block 91, #reads = 1082230 mem = 536878887 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282626, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 322, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (338.82, 154.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678094 reads in 107.340 CPU sec, 3.492 real sec
[M::process] read 678226 sequences (100000055 bp) in 3.530 CPU sec, 3.725 real sec...
sorting block 92, #reads = 1081738 mem = 536882324 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282380, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (344.00, 157.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677870 sequences (100000294 bp) in 3.091 CPU sec, 3.361 real sec...
[M::mem_process_seqs] Processed 678226 reads in 107.041 CPU sec, 3.480 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282134, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (346.65, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677870 reads in 107.888 CPU sec, 3.519 real sec
[M::process] read 677826 sequences (100000240 bp) in 3.714 CPU sec, 3.962 real sec...
sorting block 93, #reads = 1080509 mem = 536878970 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282432, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (348.20, 160.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677826 reads in 102.564 CPU sec, 3.338 real sec
[M::process] read 677672 sequences (100000278 bp) in 3.294 CPU sec, 3.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282565, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (347.72, 161.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677672 reads in 104.253 CPU sec, 3.382 real sec
sorting block 94, #reads = 1082152 mem = 536881389 peak = 999280640
[M::process] read 677802 sequences (100000268 bp) in 3.581 CPU sec, 3.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282511, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (350.41, 162.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677430 sequences (100000165 bp) in 2.982 CPU sec, 3.180 real sec...
[M::mem_process_seqs] Processed 677802 reads in 102.080 CPU sec, 3.299 real sec
sorting block 95, #reads = 1081651 mem = 536881108 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282862, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (352.94, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677430 reads in 103.373 CPU sec, 3.357 real sec
[M::process] read 677242 sequences (100000109 bp) in 3.339 CPU sec, 3.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282780, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (355.41, 164.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677242 reads in 104.235 CPU sec, 3.379 real sec
sorting block 96, #reads = 1080939 mem = 536877656 peak = 999280640
[M::process] read 685306 sequences (100000044 bp) in 3.699 CPU sec, 3.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285627, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (313.87, 156.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684230 sequences (100000161 bp) in 3.202 CPU sec, 3.341 real sec...
[M::mem_process_seqs] Processed 685306 reads in 114.268 CPU sec, 3.676 real sec
sorting block 97, #reads = 1081053 mem = 536878976 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285175, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (319.71, 158.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684230 reads in 109.840 CPU sec, 3.551 real sec
[M::process] read 683326 sequences (100000251 bp) in 3.716 CPU sec, 3.937 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285502, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 299, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (324.45, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683326 reads in 109.378 CPU sec, 3.521 real sec
[M::process] read 681896 sequences (100000072 bp) in 3.366 CPU sec, 3.545 real sec...
sorting block 98, #reads = 1083627 mem = 536880316 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284207, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 310, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (334.12, 162.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681436 sequences (100000290 bp) in 2.989 CPU sec, 3.128 real sec...
[M::mem_process_seqs] Processed 681896 reads in 103.999 CPU sec, 3.369 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283892, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 313, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (336.41, 163.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 99, #reads = 1082852 mem = 536879986 peak = 999280640
[M::mem_process_seqs] Processed 681436 reads in 102.929 CPU sec, 3.322 real sec
[M::process] read 680702 sequences (100000284 bp) in 3.741 CPU sec, 4.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283973, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (339.78, 164.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680522 sequences (100000193 bp) in 3.191 CPU sec, 3.320 real sec...
[M::mem_process_seqs] Processed 680702 reads in 103.992 CPU sec, 3.349 real sec
sorting block 100, #reads = 1083923 mem = 536878507 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283857, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (341.86, 165.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680522 reads in 103.842 CPU sec, 3.384 real sec
[M::process] read 680240 sequences (100000174 bp) in 3.285 CPU sec, 3.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283915, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 321, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (342.65, 164.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680240 reads in 102.954 CPU sec, 3.346 real sec
sorting block 101, #reads = 1082977 mem = 536878752 peak = 999280640
[M::process] read 679730 sequences (100000074 bp) in 3.342 CPU sec, 3.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283368, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (345.43, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679834 sequences (100000194 bp) in 3.080 CPU sec, 3.304 real sec...
[M::mem_process_seqs] Processed 679730 reads in 104.907 CPU sec, 3.376 real sec
sorting block 102, #reads = 1083652 mem = 536879985 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283517, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (345.99, 165.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679418 sequences (100000010 bp) in 3.006 CPU sec, 3.126 real sec...
[M::mem_process_seqs] Processed 679834 reads in 102.579 CPU sec, 3.313 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283349, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (347.32, 164.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679378 sequences (100000172 bp) in 3.230 CPU sec, 3.326 real sec...
[M::process] read 679202 sequences (100000184 bp) in 2.160 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283208, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 329, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (348.40, 165.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679378 reads in 101.890 CPU sec, 3.196 real sec
sorting block 103, #reads = 1082187 mem = 536879466 peak = 999280640
[M::process] read 679008 sequences (100000130 bp) in 2.739 CPU sec, 2.797 real sec...
[M::mem_process_seqs] Processed 679418 reads in 264.128 CPU sec, 8.374 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283310, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (349.81, 165.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 104, #reads = 1081509 mem = 536877814 peak = 999280640
[M::process] read 678840 sequences (100000170 bp) in 2.988 CPU sec, 3.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283321, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (348.79, 164.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679202 reads in 202.210 CPU sec, 6.388 real sec
[M::process] read 678542 sequences (100000099 bp) in 3.259 CPU sec, 3.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283040, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (348.21, 163.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678840 reads in 100.896 CPU sec, 3.181 real sec
sorting block 105, #reads = 1082360 mem = 536877637 peak = 999280640
[M::process] read 678546 sequences (100000276 bp) in 3.137 CPU sec, 3.226 real sec...
[M::mem_process_seqs] Processed 679008 reads in 294.854 CPU sec, 9.307 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283046, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (350.83, 164.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 106, #reads = 1081194 mem = 536883223 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283496, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (349.21, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678314 sequences (100000240 bp) in 2.816 CPU sec, 2.907 real sec...
[M::mem_process_seqs] Processed 678542 reads in 202.722 CPU sec, 6.400 real sec
[M::mem_process_seqs] Processed 678546 reads in 122.391 CPU sec, 3.864 real sec
[M::process] read 678118 sequences (100000286 bp) in 3.235 CPU sec, 3.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282690, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (349.66, 162.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 107, #reads = 1079906 mem = 536877078 peak = 999280640
[M::process] read 678304 sequences (100000301 bp) in 2.453 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282769, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (349.53, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678314 reads in 182.644 CPU sec, 5.794 real sec
[M::mem_process_seqs] Processed 678118 reads in 121.972 CPU sec, 3.846 real sec
[M::process] read 678482 sequences (100000017 bp) in 2.384 CPU sec, 2.469 real sec...
sorting block 108, #reads = 1079731 mem = 536877408 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283032, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (348.23, 162.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678346 sequences (100000050 bp) in 2.981 CPU sec, 3.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283349, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (347.04, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678304 reads in 183.021 CPU sec, 5.808 real sec
[M::mem_process_seqs] Processed 678482 reads in 123.206 CPU sec, 3.879 real sec
[M::process] read 678046 sequences (100000260 bp) in 2.318 CPU sec, 2.354 real sec...
sorting block 109, #reads = 1079543 mem = 536878595 peak = 999280640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282850, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (347.15, 161.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677978 sequences (100000108 bp) in 2.829 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283084, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (348.45, 161.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678280 sequences (100000115 bp) in 1.940 CPU sec, 1.944 real sec...
[M::mem_process_seqs] Processed 678346 reads in 182.296 CPU sec, 5.783 real sec
sorting block 110, #reads = 1078789 mem = 536878944 peak = 999280640
[M::mem_process_seqs] Processed 678046 reads in 120.052 CPU sec, 3.795 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282539, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (349.26, 162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 111, #reads = 1079631 mem = 536878917 peak = 1005146112
[M::process] read 678270 sequences (100000143 bp) in 3.137 CPU sec, 3.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282904, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (347.14, 161.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677978 reads in 181.075 CPU sec, 5.764 real sec
[M::mem_process_seqs] Processed 678280 reads in 120.939 CPU sec, 3.810 real sec
[M::process] read 678070 sequences (100000270 bp) in 3.210 CPU sec, 3.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282666, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (346.49, 160.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 112, #reads = 1079096 mem = 536881175 peak = 1005146112
[M::process] read 678342 sequences (100000250 bp) in 2.966 CPU sec, 3.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281982, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (348.32, 161.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678270 reads in 180.703 CPU sec, 5.747 real sec
[M::mem_process_seqs] Processed 678070 reads in 123.205 CPU sec, 3.880 real sec
[M::process] read 677806 sequences (100000181 bp) in 2.654 CPU sec, 2.756 real sec...
sorting block 113, #reads = 1078851 mem = 536879531 peak = 1005146112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282897, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (346.54, 160.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677870 sequences (100000125 bp) in 2.619 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282814, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (348.69, 161.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678342 reads in 151.334 CPU sec, 4.828 real sec
[M::mem_process_seqs] Processed 677806 reads in 98.459 CPU sec, 3.098 real sec
sorting block 114, #reads = 1078537 mem = 536880387 peak = 1030139904
[M::process] read 678168 sequences (100000155 bp) in 2.935 CPU sec, 3.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283043, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (349.10, 162.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282898, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (349.55, 163.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677916 sequences (100000182 bp) in 2.669 CPU sec, 2.728 real sec...
[M::mem_process_seqs] Processed 677870 reads in 158.831 CPU sec, 5.036 real sec
sorting block 115, #reads = 1080143 mem = 536878124 peak = 1030139904
[M::mem_process_seqs] Processed 678168 reads in 110.504 CPU sec, 3.474 real sec
[M::process] read 677708 sequences (100000066 bp) in 3.180 CPU sec, 3.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282683, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (351.99, 164.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 116, #reads = 1079973 mem = 536875687 peak = 1030139904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282684, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (353.13, 165.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677744 sequences (100000182 bp) in 2.827 CPU sec, 2.896 real sec...
[M::mem_process_seqs] Processed 677916 reads in 170.369 CPU sec, 5.409 real sec
[M::mem_process_seqs] Processed 677708 reads in 114.001 CPU sec, 3.595 real sec
sorting block 117, #reads = 1080244 mem = 536877641 peak = 1030139904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283174, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (354.37, 166.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677680 sequences (100000256 bp) in 3.226 CPU sec, 3.365 real sec...
[M::mem_process_seqs] Processed 677744 reads in 94.035 CPU sec, 3.067 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283199, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 337, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (355.55, 167.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677680 reads in 93.627 CPU sec, 3.020 real sec
sorting block 118, #reads = 1079936 mem = 536884219 peak = 1030139904
[M::process] read 686962 sequences (100000078 bp) in 3.474 CPU sec, 3.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285978, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 275, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (302.82, 149.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686288 sequences (100000098 bp) in 2.889 CPU sec, 2.966 real sec...
[M::mem_process_seqs] Processed 686962 reads in 98.353 CPU sec, 3.156 real sec
sorting block 119, #reads = 1080320 mem = 536878212 peak = 1030139904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286007, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (306.29, 149.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686114 sequences (100000013 bp) in 2.650 CPU sec, 2.756 real sec...
[M::mem_process_seqs] Processed 686288 reads in 97.708 CPU sec, 3.123 real sec
[M::process] read 685286 sequences (100000045 bp) in 2.408 CPU sec, 2.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285949, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (306.83, 149.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683974 sequences (100000036 bp) in 1.730 CPU sec, 1.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285621, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (309.90, 149.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683232 sequences (100000111 bp) in 1.650 CPU sec, 1.652 real sec...
[M::mem_process_seqs] Processed 686114 reads in 176.807 CPU sec, 5.554 real sec
sorting block 120, #reads = 1081273 mem = 536878493 peak = 1030139904
[M::mem_process_seqs] Processed 685286 reads in 117.268 CPU sec, 3.702 real sec
sorting block 121, #reads = 1079041 mem = 536877979 peak = 1086681088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285302, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (316.16, 151.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682630 sequences (100000149 bp) in 2.856 CPU sec, 2.995 real sec...
[M::process] read 682348 sequences (100000031 bp) in 1.790 CPU sec, 1.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284809, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (320.49, 152.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683974 reads in 171.064 CPU sec, 5.413 real sec
[M::mem_process_seqs] Processed 683232 reads in 111.791 CPU sec, 3.543 real sec
sorting block 122, #reads = 1079831 mem = 536884024 peak = 1086681088
[M::process] read 681948 sequences (100000295 bp) in 2.136 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284755, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 303, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (323.37, 153.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681414 sequences (100000015 bp) in 1.720 CPU sec, 1.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285043, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 304, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (325.69, 155.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 460, 836)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016)
[M::mem_pestat] mean and std.dev: (507.82, 270.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2606)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682630 reads in 167.952 CPU sec, 5.322 real sec
[M::mem_process_seqs] Processed 682348 reads in 109.670 CPU sec, 3.493 real sec
sorting block 123, #reads = 1079270 mem = 536881029 peak = 1086681088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284543, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (328.56, 155.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681514 sequences (100000134 bp) in 3.041 CPU sec, 3.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284494, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (332.08, 156.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681948 reads in 165.419 CPU sec, 5.263 real sec
[M::mem_process_seqs] Processed 681414 reads in 109.339 CPU sec, 3.440 real sec
sorting block 124, #reads = 1079313 mem = 536877887 peak = 1086681088
[M::process] read 681556 sequences (100000254 bp) in 2.390 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284544, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 311, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (330.32, 156.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680830 sequences (100000242 bp) in 3.087 CPU sec, 3.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283930, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (330.42, 155.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681514 reads in 166.058 CPU sec, 5.281 real sec
sorting block 125, #reads = 1079666 mem = 536882345 peak = 1086681088
[M::mem_process_seqs] Processed 681556 reads in 111.822 CPU sec, 3.521 real sec
[M::process] read 680714 sequences (100000139 bp) in 2.841 CPU sec, 2.926 real sec...
sorting block 126, #reads = 1079988 mem = 536878514 peak = 1086681088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284643, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (334.47, 156.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680164 sequences (100000219 bp) in 2.024 CPU sec, 2.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284338, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (336.09, 157.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680830 reads in 145.132 CPU sec, 4.600 real sec
[M::mem_process_seqs] Processed 680714 reads in 93.528 CPU sec, 2.939 real sec
[M::process] read 679586 sequences (100000240 bp) in 2.019 CPU sec, 2.084 real sec...
sorting block 127, #reads = 1079577 mem = 536879419 peak = 1086681088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284067, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (339.45, 159.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679724 sequences (100000119 bp) in 2.153 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284145, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (342.88, 160.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680164 reads in 142.482 CPU sec, 4.512 real sec
[M::mem_process_seqs] Processed 679586 reads in 94.065 CPU sec, 2.954 real sec
[M::process] read 679454 sequences (100000144 bp) in 1.963 CPU sec, 1.985 real sec...
sorting block 128, #reads = 1078836 mem = 536878317 peak = 1086681088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283617, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (342.12, 159.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679030 sequences (100000286 bp) in 2.437 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283447, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (343.12, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679724 reads in 143.683 CPU sec, 4.565 real sec
sorting block 129, #reads = 1079098 mem = 536877645 peak = 1086681088
[M::mem_process_seqs] Processed 679454 reads in 95.483 CPU sec, 3.000 real sec
[M::process] read 678928 sequences (100000006 bp) in 1.899 CPU sec, 1.921 real sec...
sorting block 130, #reads = 1078492 mem = 536879092 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283551, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (344.69, 161.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678754 sequences (100000032 bp) in 2.254 CPU sec, 2.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283871, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (344.02, 160.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679030 reads in 144.230 CPU sec, 4.563 real sec
[M::process] read 678664 sequences (100000178 bp) in 1.710 CPU sec, 1.705 real sec...
[M::mem_process_seqs] Processed 678928 reads in 94.233 CPU sec, 2.971 real sec
sorting block 131, #reads = 1079481 mem = 536878975 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283397, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (345.09, 161.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678636 sequences (100000233 bp) in 2.634 CPU sec, 2.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283461, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (346.93, 162.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678754 reads in 141.546 CPU sec, 4.467 real sec
[M::process] read 678576 sequences (100000166 bp) in 2.035 CPU sec, 2.079 real sec...
[M::mem_process_seqs] Processed 678664 reads in 93.054 CPU sec, 2.923 real sec
sorting block 132, #reads = 1079264 mem = 536876876 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283430, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (347.09, 162.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678548 sequences (100000200 bp) in 2.484 CPU sec, 2.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283042, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (347.90, 162.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678636 reads in 138.703 CPU sec, 4.408 real sec
[M::mem_process_seqs] Processed 678576 reads in 91.556 CPU sec, 2.881 real sec
sorting block 133, #reads = 1080070 mem = 536878963 peak = 1132691456
[M::process] read 678718 sequences (100000282 bp) in 2.101 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283365, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (346.82, 161.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678140 sequences (100000019 bp) in 2.299 CPU sec, 2.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282989, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 325, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (344.55, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678548 reads in 142.865 CPU sec, 4.522 real sec
[M::process] read 677820 sequences (100000117 bp) in 1.867 CPU sec, 1.884 real sec...
sorting block 134, #reads = 1080222 mem = 536883749 peak = 1132691456
[M::mem_process_seqs] Processed 678718 reads in 93.776 CPU sec, 2.951 real sec
sorting block 135, #reads = 1078968 mem = 536879357 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283192, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (349.47, 163.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677876 sequences (100000144 bp) in 2.270 CPU sec, 2.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282837, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (351.59, 164.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678140 reads in 137.886 CPU sec, 4.367 real sec
[M::process] read 677732 sequences (100000063 bp) in 1.912 CPU sec, 1.922 real sec...
[M::mem_process_seqs] Processed 677820 reads in 90.226 CPU sec, 2.836 real sec
sorting block 136, #reads = 1079172 mem = 536881006 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282730, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (351.44, 164.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677806 sequences (100000190 bp) in 2.398 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282800, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (353.13, 165.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677876 reads in 136.126 CPU sec, 4.307 real sec
[M::mem_process_seqs] Processed 677732 reads in 89.981 CPU sec, 2.823 real sec
[M::process] read 678072 sequences (100000265 bp) in 2.186 CPU sec, 2.235 real sec...
sorting block 137, #reads = 1081367 mem = 536881843 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282533, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (353.93, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678584 sequences (100000114 bp) in 2.548 CPU sec, 2.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282732, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (353.04, 167.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677806 reads in 139.262 CPU sec, 4.444 real sec
[M::mem_process_seqs] Processed 678072 reads in 93.058 CPU sec, 2.931 real sec
sorting block 138, #reads = 1080580 mem = 536879195 peak = 1132691456
[M::process] read 684822 sequences (100000063 bp) in 2.428 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283003, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 328, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (349.96, 168.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684266 sequences (100000164 bp) in 2.520 CPU sec, 2.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284425, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 290, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (315.47, 154.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678584 reads in 153.698 CPU sec, 4.885 real sec
sorting block 139, #reads = 1079198 mem = 536879799 peak = 1132691456
[M::mem_process_seqs] Processed 684822 reads in 101.745 CPU sec, 3.204 real sec
[M::process] read 683494 sequences (100000074 bp) in 2.321 CPU sec, 2.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285094, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (316.38, 153.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 140, #reads = 1074397 mem = 536881675 peak = 1132691456
[M::process] read 683412 sequences (100000279 bp) in 2.520 CPU sec, 2.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284882, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (318.87, 153.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684266 reads in 141.193 CPU sec, 4.505 real sec
[M::mem_process_seqs] Processed 683494 reads in 91.952 CPU sec, 2.901 real sec
[M::process] read 682596 sequences (100000148 bp) in 2.865 CPU sec, 3.043 real sec...
sorting block 141, #reads = 1084808 mem = 536879400 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283931, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (321.11, 154.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284009, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 303, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (324.09, 154.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681962 sequences (100000191 bp) in 2.021 CPU sec, 2.079 real sec...
[M::mem_process_seqs] Processed 683412 reads in 140.580 CPU sec, 4.484 real sec
[M::mem_process_seqs] Processed 682596 reads in 90.892 CPU sec, 2.868 real sec
sorting block 142, #reads = 1083893 mem = 536881315 peak = 1132691456
[M::process] read 681594 sequences (100000236 bp) in 2.290 CPU sec, 2.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284208, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 306, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (326.61, 155.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681160 sequences (100000093 bp) in 1.917 CPU sec, 1.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283926, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 308, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (328.01, 154.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681962 reads in 135.517 CPU sec, 4.298 real sec
[M::mem_process_seqs] Processed 681594 reads in 87.628 CPU sec, 2.755 real sec
sorting block 143, #reads = 1084581 mem = 536878846 peak = 1132691456
[M::process] read 681006 sequences (100000255 bp) in 2.666 CPU sec, 2.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283503, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 311, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (330.22, 155.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283719, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (330.94, 154.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681160 reads in 135.589 CPU sec, 4.329 real sec
[M::process] read 680604 sequences (100000163 bp) in 2.663 CPU sec, 2.775 real sec...
sorting block 144, #reads = 1083582 mem = 536877938 peak = 1132691456
[M::mem_process_seqs] Processed 681006 reads in 88.254 CPU sec, 2.797 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283394, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (333.09, 155.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680604 reads in 75.060 CPU sec, 2.478 real sec
sorting block 145, #reads = 1082700 mem = 536879850 peak = 1132691456
[M::process] read 680720 sequences (100000017 bp) in 3.215 CPU sec, 3.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284295, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (334.61, 156.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680720 reads in 83.843 CPU sec, 2.717 real sec
[M::process] read 680352 sequences (100000051 bp) in 2.790 CPU sec, 2.989 real sec...
sorting block 146, #reads = 1083317 mem = 536878941 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283295, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (335.96, 156.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680742 sequences (100000209 bp) in 2.273 CPU sec, 2.334 real sec...
[M::mem_process_seqs] Processed 680352 reads in 77.583 CPU sec, 2.504 real sec
sorting block 147, #reads = 1081003 mem = 536881079 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283886, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 315, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (333.59, 156.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680574 sequences (100000141 bp) in 2.093 CPU sec, 2.168 real sec...
[M::mem_process_seqs] Processed 680742 reads in 75.930 CPU sec, 2.434 real sec
[M::process] read 680212 sequences (100000019 bp) in 2.108 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283972, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (334.32, 156.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680168 sequences (100000200 bp) in 1.570 CPU sec, 1.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283210, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (334.38, 155.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680574 reads in 135.384 CPU sec, 4.247 real sec
[M::mem_process_seqs] Processed 680212 reads in 88.209 CPU sec, 2.766 real sec
sorting block 148, #reads = 1082664 mem = 536879305 peak = 1132691456
[M::process] read 679962 sequences (100000026 bp) in 2.148 CPU sec, 2.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283463, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 318, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (334.93, 154.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 149, #reads = 1082574 mem = 536876433 peak = 1132691456
[M::process] read 680306 sequences (100000137 bp) in 2.659 CPU sec, 2.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283528, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (337.01, 155.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680168 reads in 137.128 CPU sec, 4.373 real sec
[M::mem_process_seqs] Processed 679962 reads in 89.573 CPU sec, 2.832 real sec
sorting block 150, #reads = 1082658 mem = 536878056 peak = 1132691456
[M::process] read 680752 sequences (100000243 bp) in 2.681 CPU sec, 2.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283670, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (333.70, 155.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283587, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 312, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (329.76, 154.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679874 sequences (100000294 bp) in 2.194 CPU sec, 2.243 real sec...
[M::mem_process_seqs] Processed 680306 reads in 139.378 CPU sec, 4.427 real sec
[M::mem_process_seqs] Processed 680752 reads in 90.753 CPU sec, 2.869 real sec
sorting block 151, #reads = 1082155 mem = 536878073 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283456, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (339.28, 157.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679874 reads in 81.937 CPU sec, 2.681 real sec
[M::process] read 679304 sequences (100000264 bp) in 3.281 CPU sec, 3.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283243, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (342.51, 158.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 152, #reads = 1080607 mem = 536879846 peak = 1132691456
[M::mem_process_seqs] Processed 679304 reads in 79.742 CPU sec, 2.600 real sec
[M::process] read 679168 sequences (100000042 bp) in 2.756 CPU sec, 2.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283579, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (342.37, 158.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679396 sequences (100000060 bp) in 2.038 CPU sec, 2.120 real sec...
[M::mem_process_seqs] Processed 679168 reads in 80.655 CPU sec, 2.584 real sec
sorting block 153, #reads = 1081051 mem = 536877575 peak = 1132691456
[M::process] read 678926 sequences (100000013 bp) in 2.144 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282927, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (341.04, 157.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678660 sequences (100000130 bp) in 1.600 CPU sec, 1.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283070, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (344.14, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679396 reads in 144.285 CPU sec, 4.526 real sec
[M::mem_process_seqs] Processed 678926 reads in 93.981 CPU sec, 2.945 real sec
[M::process] read 679130 sequences (100000175 bp) in 1.824 CPU sec, 1.841 real sec...
sorting block 154, #reads = 1079036 mem = 536879057 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283174, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (345.52, 160.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679234 sequences (100000173 bp) in 2.341 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283487, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (341.19, 159.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678660 reads in 144.380 CPU sec, 4.564 real sec
[M::process] read 678834 sequences (100000181 bp) in 1.740 CPU sec, 1.743 real sec...
sorting block 155, #reads = 1078627 mem = 536880370 peak = 1132691456
[M::mem_process_seqs] Processed 679130 reads in 95.276 CPU sec, 3.005 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282521, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 320, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (338.40, 156.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678520 sequences (100000138 bp) in 2.343 CPU sec, 2.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283217, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (340.96, 157.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678672 sequences (100000122 bp) in 1.750 CPU sec, 1.752 real sec...
[M::mem_process_seqs] Processed 679234 reads in 148.070 CPU sec, 4.680 real sec
sorting block 156, #reads = 1078426 mem = 536880140 peak = 1132691456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282830, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (345.45, 161.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678374 sequences (100000222 bp) in 2.130 CPU sec, 2.139 real sec...
[M::mem_process_seqs] Processed 678520 reads in 81.330 CPU sec, 2.559 real sec
sorting block 157, #reads = 1076756 mem = 536878268 peak = 1134632960
[M::mem_process_seqs] Processed 678834 reads in 202.374 CPU sec, 6.394 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283306, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (344.99, 161.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678628 sequences (100000300 bp) in 2.690 CPU sec, 2.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282771, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (346.25, 160.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678672 reads in 161.911 CPU sec, 5.130 real sec
sorting block 158, #reads = 1078687 mem = 536878722 peak = 1134632960
[M::mem_process_seqs] Processed 678374 reads in 97.002 CPU sec, 3.059 real sec
[M::process] read 678166 sequences (100000016 bp) in 2.081 CPU sec, 2.129 real sec...
sorting block 159, #reads = 1078676 mem = 536879959 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282831, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (345.42, 161.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678388 sequences (100000188 bp) in 2.283 CPU sec, 2.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283107, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (348.20, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678628 reads in 150.914 CPU sec, 4.764 real sec
[M::process] read 679062 sequences (100000214 bp) in 1.799 CPU sec, 1.800 real sec...
[M::mem_process_seqs] Processed 678166 reads in 98.430 CPU sec, 3.100 real sec
sorting block 160, #reads = 1077149 mem = 536878187 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282772, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (347.99, 163.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685836 sequences (100000262 bp) in 2.662 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283143, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (346.52, 164.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678388 reads in 159.861 CPU sec, 5.054 real sec
[M::process] read 688008 sequences (100000250 bp) in 2.035 CPU sec, 2.045 real sec...
[M::mem_process_seqs] Processed 679062 reads in 111.281 CPU sec, 3.508 real sec
sorting block 161, #reads = 1075323 mem = 536878520 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285317, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (310.66, 153.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686834 sequences (100000209 bp) in 2.961 CPU sec, 3.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286371, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 271, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (297.23, 144.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685836 reads in 159.859 CPU sec, 5.063 real sec
[M::mem_process_seqs] Processed 688008 reads in 103.707 CPU sec, 3.260 real sec
[M::process] read 685568 sequences (100000249 bp) in 2.322 CPU sec, 2.391 real sec...
sorting block 162, #reads = 1068923 mem = 536879612 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286117, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 279, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (303.21, 146.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685148 sequences (100000235 bp) in 2.392 CPU sec, 2.469 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285593, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 286, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (307.44, 146.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686834 reads in 156.666 CPU sec, 4.996 real sec
sorting block 163, #reads = 1080936 mem = 536878279 peak = 1134632960
[M::mem_process_seqs] Processed 685568 reads in 104.060 CPU sec, 3.277 real sec
[M::process] read 684178 sequences (100000074 bp) in 2.093 CPU sec, 2.129 real sec...
sorting block 164, #reads = 1080232 mem = 536878787 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285567, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (309.13, 146.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683542 sequences (100000003 bp) in 2.431 CPU sec, 2.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285539, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (313.87, 147.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682880 sequences (100000087 bp) in 1.790 CPU sec, 1.784 real sec...
[M::mem_process_seqs] Processed 685148 reads in 157.260 CPU sec, 4.977 real sec
[M::mem_process_seqs] Processed 684178 reads in 102.869 CPU sec, 3.248 real sec
sorting block 165, #reads = 1078931 mem = 536878301 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285348, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (316.97, 148.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682466 sequences (100000231 bp) in 2.628 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284908, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 302, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (321.40, 151.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683542 reads in 154.593 CPU sec, 4.891 real sec
[M::mem_process_seqs] Processed 682880 reads in 101.255 CPU sec, 3.179 real sec
[M::process] read 682108 sequences (100000107 bp) in 2.405 CPU sec, 2.424 real sec...
sorting block 166, #reads = 1077849 mem = 536879979 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285130, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 306, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (323.18, 150.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681330 sequences (100000092 bp) in 2.538 CPU sec, 2.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284812, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 307, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (324.33, 150.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682080 sequences (100000025 bp) in 1.820 CPU sec, 1.818 real sec...
[M::mem_process_seqs] Processed 682466 reads in 154.551 CPU sec, 4.885 real sec
sorting block 167, #reads = 1078042 mem = 536878012 peak = 1134632960
[M::mem_process_seqs] Processed 682108 reads in 101.100 CPU sec, 3.189 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284061, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 314, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (331.05, 154.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 168, #reads = 1077598 mem = 536878953 peak = 1134632960
[M::process] read 682008 sequences (100000036 bp) in 2.401 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284850, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 310, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (328.63, 154.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681186 sequences (100000056 bp) in 1.818 CPU sec, 1.825 real sec...
[M::mem_process_seqs] Processed 681330 reads in 152.284 CPU sec, 4.818 real sec
[M::mem_process_seqs] Processed 682080 reads in 101.634 CPU sec, 3.208 real sec
sorting block 169, #reads = 1078083 mem = 536879678 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285023, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 310, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (328.53, 154.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681128 sequences (100000018 bp) in 2.551 CPU sec, 2.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284238, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (333.60, 156.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681548 sequences (100000134 bp) in 1.820 CPU sec, 1.816 real sec...
[M::mem_process_seqs] Processed 682008 reads in 153.621 CPU sec, 4.854 real sec
[M::mem_process_seqs] Processed 681186 reads in 100.506 CPU sec, 3.171 real sec
sorting block 170, #reads = 1077228 mem = 536878124 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283997, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (334.49, 157.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681942 sequences (100000028 bp) in 2.380 CPU sec, 2.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284312, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (330.23, 154.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681128 reads in 149.227 CPU sec, 4.731 real sec
[M::process] read 680436 sequences (100000132 bp) in 1.898 CPU sec, 1.911 real sec...
[M::mem_process_seqs] Processed 681548 reads in 98.561 CPU sec, 3.107 real sec
sorting block 171, #reads = 1077654 mem = 536878282 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284138, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 304, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (324.84, 153.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679862 sequences (100000221 bp) in 2.622 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283712, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 314, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (331.64, 153.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681942 reads in 152.183 CPU sec, 4.822 real sec
sorting block 172, #reads = 1079553 mem = 536881384 peak = 1134632960
[M::process] read 679272 sequences (100000023 bp) in 2.014 CPU sec, 2.024 real sec...
[M::mem_process_seqs] Processed 680436 reads in 99.213 CPU sec, 3.113 real sec
sorting block 173, #reads = 1078799 mem = 536876539 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283010, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (334.28, 154.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679322 sequences (100000171 bp) in 2.220 CPU sec, 2.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283037, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 322, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (338.63, 155.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679862 reads in 151.066 CPU sec, 4.798 real sec
[M::process] read 679078 sequences (100000083 bp) in 1.818 CPU sec, 1.820 real sec...
[M::mem_process_seqs] Processed 679272 reads in 98.071 CPU sec, 3.089 real sec
sorting block 174, #reads = 1078229 mem = 536880878 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283040, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 321, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (337.80, 154.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678776 sequences (100000168 bp) in 2.642 CPU sec, 2.728 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282963, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 320, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (336.99, 154.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679322 reads in 149.810 CPU sec, 4.735 real sec
[M::process] read 679052 sequences (100000015 bp) in 1.809 CPU sec, 1.817 real sec...
[M::mem_process_seqs] Processed 679078 reads in 97.254 CPU sec, 3.069 real sec
sorting block 175, #reads = 1079296 mem = 536877652 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282282, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 322, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (338.25, 154.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679076 sequences (100000161 bp) in 2.348 CPU sec, 2.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282835, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 318, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (335.60, 154.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678716 sequences (100000226 bp) in 1.780 CPU sec, 1.783 real sec...
[M::mem_process_seqs] Processed 678776 reads in 148.287 CPU sec, 4.691 real sec
sorting block 176, #reads = 1078050 mem = 536879479 peak = 1134632960
[M::mem_process_seqs] Processed 679052 reads in 96.920 CPU sec, 3.051 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282799, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 316, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (333.07, 152.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678508 sequences (100000131 bp) in 2.520 CPU sec, 2.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282766, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 319, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (336.95, 154.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678332 sequences (100000213 bp) in 1.860 CPU sec, 1.865 real sec...
[M::mem_process_seqs] Processed 679076 reads in 148.016 CPU sec, 4.672 real sec
sorting block 177, #reads = 1079454 mem = 536879870 peak = 1134632960
[M::mem_process_seqs] Processed 678716 reads in 96.066 CPU sec, 3.026 real sec
sorting block 178, #reads = 1078213 mem = 536880709 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282565, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (338.67, 155.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677910 sequences (100000157 bp) in 2.379 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282768, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (339.56, 156.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678508 reads in 149.545 CPU sec, 4.738 real sec
[M::process] read 677960 sequences (100000162 bp) in 1.836 CPU sec, 1.842 real sec...
[M::mem_process_seqs] Processed 678332 reads in 97.560 CPU sec, 3.072 real sec
sorting block 179, #reads = 1080051 mem = 536877880 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282688, 0, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (1287, 1287, 1287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1287, 1287)
[M::mem_pestat] mean and std.dev: (1287.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1287, 1287)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (347.18, 160.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677728 sequences (100000110 bp) in 2.444 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282676, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (349.01, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677910 reads in 140.428 CPU sec, 4.435 real sec
[M::process] read 677546 sequences (100000300 bp) in 1.760 CPU sec, 1.765 real sec...
[M::mem_process_seqs] Processed 677960 reads in 90.360 CPU sec, 2.846 real sec
sorting block 180, #reads = 1080235 mem = 536880092 peak = 1134632960
[M::process] read 677444 sequences (100000283 bp) in 2.205 CPU sec, 2.263 real sec...
[M::process] read 678088 sequences (100000260 bp) in 1.950 CPU sec, 1.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282846, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (355.58, 166.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677444 reads in 73.966 CPU sec, 2.397 real sec
sorting block 181, #reads = 1080108 mem = 536878043 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282539, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (353.13, 165.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 41748 sequences (6138949 bp) in 0.402 CPU sec, 0.405 real sec...
[M::mem_process_seqs] Processed 677546 reads in 150.965 CPU sec, 5.515 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282536, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (349.18, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282650, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (352.52, 165.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677728 reads in 289.184 CPU sec, 9.971 real sec
sorting block 182, #reads = 1080402 mem = 536882465 peak = 1134632960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 17399, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (338.33, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 41748 reads in 5.875 CPU sec, 0.198 real sec
[M::mem_process_seqs] Processed 678088 reads in 116.859 CPU sec, 4.137 real sec
sorting block 183, #reads = 1080283 mem = 536879140 peak = 1196638208
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 198433392
overall: 69740765184 mem 29333.655 user 1085.876 sys 1058.891 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cVw-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1058.931 sec; CPU: 30419.534 sec
sorting block 184, #reads = 276695 mem = 138135375 peak = 1196638208
uniq header count 1
merging 185 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 10792692884
output reads: 199289852
bam_mem_sort: 185 calls 115.681 user 4.706 sys 120.732 real
bam_write: 185 calls 76.941 user 5.363 sys 89.211 real
execute: 1 calls 61.071 user 2.966 sys 1139.668 real
merge_files: 1 calls 61.038 user 2.920 sys 80.495 real
parse_chunk: 2949 calls 161.789 user 40.558 sys 206.161 real
read_chunk: 7944 calls 86.789 user 68.973 sys 974.948 real
sort_block: 1 calls 67.593 user 68.567 sys 942.621 real
write_chunk: 4993 calls 60.573 user 2.469 sys 79.249 real
overall: 3341017088 mem 3209.600 user 147.954 sys 1139.696 real
ls: cannot access '*.cram': No such file or directory