cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_FRC-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 No more license available for sentieon:klib No more license available for sentieon:klib [M::bwa_idx_load_from_disk] read 3171 ALT contigs No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib [M::main_mem] bwa index loaded in 1651.273 CPU sec, 1076.871 real sec [M::process] read 698890 sequences (100000287 bp) in 0.982 CPU sec, 0.983 real sec... [M::process] read 695450 sequences (100000019 bp) in 1.180 CPU sec, 1.183 real sec... [M::process] read 694600 sequences (100000117 bp) in 1.160 CPU sec, 1.164 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284300, 12, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 402, 928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2378) [M::mem_pestat] mean and std.dev: (427.57, 361.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 251, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (277.78, 142.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (278, 799, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2264) [M::mem_pestat] mean and std.dev: (644.17, 424.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 290, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267) [M::mem_pestat] mean and std.dev: (305.05, 169.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694250 sequences (100000101 bp) in 1.128 CPU sec, 1.127 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 283037, 18, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 296, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (338.29, 199.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.61, 148.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 824, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2888) [M::mem_pestat] mean and std.dev: (760.88, 584.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 273, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (267.97, 96.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 283057, 37, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 272, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (300.83, 195.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (290.91, 147.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 298, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2675) [M::mem_pestat] mean and std.dev: (457.72, 411.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 305, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (322.60, 155.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698890 reads in 231.901 CPU sec, 7.391 real sec [M::mem_process_seqs] Processed 694600 reads in 156.934 CPU sec, 5.062 real sec [M::mem_process_seqs] Processed 695450 reads in 172.863 CPU sec, 5.582 real sec sorting block 0, #reads = 1076883 mem = 536878103 peak = 611078144 [M::process] read 693806 sequences (100000047 bp) in 1.650 CPU sec, 1.767 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282699, 16, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 334, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516) [M::mem_pestat] mean and std.dev: (426.09, 261.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (295.74, 149.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 1380, 6157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18255) [M::mem_pestat] mean and std.dev: (2381.75, 2987.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 328, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238) [M::mem_pestat] mean and std.dev: (357.08, 202.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693162 sequences (100000036 bp) in 1.284 CPU sec, 1.308 real sec... [M::process] read 692894 sequences (100000119 bp) in 1.120 CPU sec, 1.123 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282622, 19, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 234, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (223.15, 100.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (296.67, 149.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 290, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2865) [M::mem_pestat] mean and std.dev: (497.05, 472.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3796) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 292, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (246.90, 75.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694250 reads in 143.217 CPU sec, 4.547 real sec sorting block 1, #reads = 1075887 mem = 536879402 peak = 802967552 [M::mem_process_seqs] Processed 693806 reads in 93.961 CPU sec, 2.987 real sec sorting block 2, #reads = 1075731 mem = 536875999 peak = 916914176 [M::process] read 692096 sequences (100000186 bp) in 1.616 CPU sec, 1.724 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 282581, 19, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (242.94, 147.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 276, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (298.67, 149.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 475, 2326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6526) [M::mem_pestat] mean and std.dev: (1354.11, 1572.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 388, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (406.90, 214.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691776 sequences (100000077 bp) in 1.224 CPU sec, 1.238 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 282189, 28, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 295, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2302) [M::mem_pestat] mean and std.dev: (519.42, 510.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 279, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (301.44, 151.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (401, 1301, 5089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14465) [M::mem_pestat] mean and std.dev: (2065.07, 2029.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 342, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (361.24, 165.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281504, 13, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 493, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4809) [M::mem_pestat] mean and std.dev: (858.55, 866.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 281, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (302.47, 151.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (493, 896, 8819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25471) [M::mem_pestat] mean and std.dev: (3333.08, 3620.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 33797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 298, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (272.56, 108.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693162 reads in 201.960 CPU sec, 6.483 real sec [M::mem_process_seqs] Processed 692894 reads in 158.618 CPU sec, 5.130 real sec [M::mem_process_seqs] Processed 692096 reads in 169.341 CPU sec, 5.487 real sec sorting block 3, #reads = 1075425 mem = 536876913 peak = 916914176 [M::process] read 691936 sequences (100000289 bp) in 1.671 CPU sec, 1.795 real sec... sorting block 4, #reads = 1073988 mem = 536882997 peak = 916914176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281443, 23, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 398, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3913) [M::mem_pestat] mean and std.dev: (826.37, 955.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (304.94, 152.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1500, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4578) [M::mem_pestat] mean and std.dev: (1406.91, 849.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5883) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 383, 908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2308) [M::mem_pestat] mean and std.dev: (605.91, 621.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691518 sequences (100000039 bp) in 1.414 CPU sec, 1.454 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282480, 23, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (303.56, 171.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 282, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (304.57, 153.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (642, 836, 3995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10701) [M::mem_pestat] mean and std.dev: (2172.52, 2173.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 256, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1600) [M::mem_pestat] mean and std.dev: (324.54, 202.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691026 sequences (100000123 bp) in 1.200 CPU sec, 1.198 real sec... [M::mem_process_seqs] Processed 691776 reads in 145.346 CPU sec, 4.623 real sec [M::mem_process_seqs] Processed 691936 reads in 93.164 CPU sec, 3.025 real sec sorting block 5, #reads = 1074343 mem = 536883238 peak = 916914176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 282169, 14, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 348, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (301.50, 115.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (305.06, 152.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 580, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2821) [M::mem_pestat] mean and std.dev: (461.50, 455.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 301, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (371.50, 257.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690658 sequences (100000172 bp) in 1.771 CPU sec, 1.910 real sec... [M::process] read 690134 sequences (100000004 bp) in 1.260 CPU sec, 1.256 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 281600, 27, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 484, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1887) [M::mem_pestat] mean and std.dev: (505.04, 330.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (308.59, 155.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (420, 999, 2481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6603) [M::mem_pestat] mean and std.dev: (1182.92, 977.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 256, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265) [M::mem_pestat] mean and std.dev: (326.31, 200.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1620) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691518 reads in 145.121 CPU sec, 4.693 real sec [M::mem_process_seqs] Processed 691026 reads in 92.890 CPU sec, 3.027 real sec sorting block 6, #reads = 1075967 mem = 536877119 peak = 916914176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281735, 29, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 389, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3975) [M::mem_pestat] mean and std.dev: (760.86, 645.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (310.53, 155.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1355, 1794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4186) [M::mem_pestat] mean and std.dev: (1205.96, 836.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (220.67, 59.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689982 sequences (100000147 bp) in 1.612 CPU sec, 1.709 real sec... [M::process] read 690096 sequences (100000194 bp) in 1.280 CPU sec, 1.277 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 282089, 15, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 270, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (287.75, 195.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (310.72, 155.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 467, 2296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6326) [M::mem_pestat] mean and std.dev: (910.86, 1100.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 303, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (359.69, 193.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690658 reads in 145.209 CPU sec, 4.699 real sec sorting block 7, #reads = 1075147 mem = 536879335 peak = 916914176 [M::mem_process_seqs] Processed 690134 reads in 92.938 CPU sec, 3.013 real sec sorting block 8, #reads = 1073805 mem = 536886102 peak = 916914176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281443, 28, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 436, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2039) [M::mem_pestat] mean and std.dev: (514.33, 390.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (312.34, 156.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (854, 1148, 2433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5591) [M::mem_pestat] mean and std.dev: (1400.59, 1180.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7170) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 337, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (318.15, 146.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690146 sequences (100000029 bp) in 1.812 CPU sec, 1.919 real sec... [M::process] read 690074 sequences (100000073 bp) in 1.320 CPU sec, 1.315 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 281965, 16, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 272, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (361.84, 257.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (311.16, 155.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (462, 736, 1615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3921) [M::mem_pestat] mean and std.dev: (765.21, 496.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5074) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 356, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (480.72, 429.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689982 reads in 146.574 CPU sec, 4.725 real sec [M::mem_process_seqs] Processed 690096 reads in 93.455 CPU sec, 2.975 real sec sorting block 9, #reads = 1074844 mem = 536877974 peak = 916914176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 281318, 13, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 350, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1565) [M::mem_pestat] mean and std.dev: (406.34, 316.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (310.42, 155.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (525, 827, 2308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5874) [M::mem_pestat] mean and std.dev: (1281.85, 946.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 428, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339) [M::mem_pestat] mean and std.dev: (432.64, 252.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689534 sequences (100000263 bp) in 1.819 CPU sec, 1.932 real sec... [M::process] read 689402 sequences (100000166 bp) in 1.330 CPU sec, 1.331 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281504, 21, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 221, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (282.06, 147.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (310.28, 154.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (360, 1007, 2139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5697) [M::mem_pestat] mean and std.dev: (1225.86, 1032.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 403, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2894) [M::mem_pestat] mean and std.dev: (574.98, 526.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690146 reads in 145.248 CPU sec, 4.629 real sec [M::mem_process_seqs] Processed 690074 reads in 94.825 CPU sec, 3.221 real sec sorting block 10, #reads = 1074772 mem = 536877672 peak = 916914176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 281429, 12, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 327, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (300.34, 182.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 287, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (308.70, 152.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 1537, 8138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21406) [M::mem_pestat] mean and std.dev: (3419.17, 3128.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 300, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032) [M::mem_pestat] mean and std.dev: (309.68, 181.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689292 sequences (100000016 bp) in 1.909 CPU sec, 2.017 real sec... [M::process] read 689474 sequences (100000204 bp) in 1.340 CPU sec, 1.339 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 280789, 12, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 308, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2615) [M::mem_pestat] mean and std.dev: (511.23, 520.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 288, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (308.89, 151.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (289, 681, 4212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12058) [M::mem_pestat] mean and std.dev: (1789.42, 2088.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15981) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 215, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (295.30, 188.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689534 reads in 149.255 CPU sec, 4.951 real sec [M::mem_process_seqs] Processed 689402 reads in 95.063 CPU sec, 3.034 real sec sorting block 11, #reads = 1072644 mem = 536878390 peak = 916914176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281111, 19, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 314, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (311.10, 173.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 287, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (307.93, 151.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (776, 904, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3713) [M::mem_pestat] mean and std.dev: (1105.56, 549.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 320, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (310.67, 180.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689802 sequences (100000086 bp) in 1.838 CPU sec, 1.949 real sec... [M::process] read 689802 sequences (100000154 bp) in 1.340 CPU sec, 1.343 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281205, 11, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 210, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970) [M::mem_pestat] mean and std.dev: (410.02, 365.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 286, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (305.68, 149.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1394, 1798, 5726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14390) [M::mem_pestat] mean and std.dev: (2880.73, 3184.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18722) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 335, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1912) [M::mem_pestat] mean and std.dev: (464.16, 337.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2471) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689292 reads in 149.834 CPU sec, 4.856 real sec sorting block 12, #reads = 1073647 mem = 536878292 peak = 916914176 [M::mem_process_seqs] Processed 689474 reads in 97.772 CPU sec, 3.130 real sec sorting block 13, #reads = 1072682 mem = 536877696 peak = 945930240 [M::process] read 689956 sequences (100000062 bp) in 1.940 CPU sec, 2.051 real sec... [M::process] read 690006 sequences (100000175 bp) in 1.330 CPU sec, 1.336 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 280767, 23, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 269, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (323.93, 231.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 283, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (303.20, 147.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 470, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (520.55, 399.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 296, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (277.89, 117.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689802 reads in 79.482 CPU sec, 2.548 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 280500, 22, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 317, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (308.17, 201.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 283, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (304.15, 148.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (85, 847, 1749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5077) [M::mem_pestat] mean and std.dev: (1099.10, 1293.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 284, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (272.63, 100.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690180 sequences (100000054 bp) in 1.475 CPU sec, 1.484 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 281236, 26, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 502, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2707) [M::mem_pestat] mean and std.dev: (720.41, 648.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 284, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (303.53, 148.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (708, 1388, 1976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4512) [M::mem_pestat] mean and std.dev: (1285.46, 727.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5780) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 336, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2174) [M::mem_pestat] mean and std.dev: (500.73, 442.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689802 reads in 226.851 CPU sec, 7.252 real sec sorting block 14, #reads = 1072058 mem = 536880746 peak = 945930240 [M::mem_process_seqs] Processed 689956 reads in 112.428 CPU sec, 3.711 real sec sorting block 15, #reads = 1071463 mem = 536879672 peak = 956784640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 280843, 23, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 273, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (273.57, 135.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 284, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (303.58, 148.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (367, 452, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1837) [M::mem_pestat] mean and std.dev: (509.85, 311.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 447, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2661) [M::mem_pestat] mean and std.dev: (500.43, 368.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689662 sequences (100000048 bp) in 2.079 CPU sec, 2.234 real sec... [M::process] read 689874 sequences (100000218 bp) in 1.370 CPU sec, 1.367 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280919, 13, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 466, 1496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4066) [M::mem_pestat] mean and std.dev: (913.64, 968.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 283, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (303.16, 148.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (536, 551, 1435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3233) [M::mem_pestat] mean and std.dev: (876.69, 774.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 263, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1545) [M::mem_pestat] mean and std.dev: (305.05, 185.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690006 reads in 149.569 CPU sec, 4.836 real sec [M::mem_process_seqs] Processed 690180 reads in 96.243 CPU sec, 3.155 real sec sorting block 16, #reads = 1072924 mem = 536876784 peak = 956784640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 280977, 15, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 229, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125) [M::mem_pestat] mean and std.dev: (303.50, 202.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 284, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (304.24, 148.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 393, 2892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8322) [M::mem_pestat] mean and std.dev: (1274.53, 1373.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 371, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2688) [M::mem_pestat] mean and std.dev: (440.48, 317.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689670 sequences (100000071 bp) in 2.123 CPU sec, 2.315 real sec... [M::process] read 689294 sequences (100000213 bp) in 1.320 CPU sec, 1.320 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281240, 23, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 265, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (298.07, 183.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 284, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (304.56, 149.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (745, 1044, 1889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4177) [M::mem_pestat] mean and std.dev: (1322.57, 986.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 307, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1745) [M::mem_pestat] mean and std.dev: (337.15, 216.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689662 reads in 149.344 CPU sec, 4.853 real sec [M::mem_process_seqs] Processed 689874 reads in 95.473 CPU sec, 3.097 real sec sorting block 17, #reads = 1070694 mem = 536884150 peak = 956784640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281543, 17, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 583, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4003) [M::mem_pestat] mean and std.dev: (757.15, 662.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 287, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (306.70, 150.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (530, 530, 1719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4097) [M::mem_pestat] mean and std.dev: (925.82, 714.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 279, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (255.20, 79.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688890 sequences (100000284 bp) in 1.884 CPU sec, 1.995 real sec... [M::process] read 693502 sequences (100000097 bp) in 1.340 CPU sec, 1.347 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 280708, 28, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 275, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (295.91, 188.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (310.27, 152.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 707, 1917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5223) [M::mem_pestat] mean and std.dev: (759.16, 744.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (291, 380, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3897) [M::mem_pestat] mean and std.dev: (704.18, 782.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689670 reads in 150.757 CPU sec, 4.869 real sec sorting block 18, #reads = 1071610 mem = 536877521 peak = 956784640 [M::mem_process_seqs] Processed 689294 reads in 96.099 CPU sec, 3.136 real sec sorting block 19, #reads = 1069918 mem = 536879158 peak = 956784640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 280216, 29, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 431, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3869) [M::mem_pestat] mean and std.dev: (702.04, 806.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 290, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (309.73, 151.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 631, 6045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17819) [M::mem_pestat] mean and std.dev: (2201.79, 3059.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 233, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1400) [M::mem_pestat] mean and std.dev: (356.15, 357.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695092 sequences (100000229 bp) in 1.874 CPU sec, 1.981 real sec... [M::process] read 695072 sequences (100000085 bp) in 1.340 CPU sec, 1.340 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 281801, 16, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 334, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1786) [M::mem_pestat] mean and std.dev: (386.42, 293.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (296.16, 148.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 382, 1429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4091) [M::mem_pestat] mean and std.dev: (550.87, 616.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 308, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2101) [M::mem_pestat] mean and std.dev: (458.04, 419.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688890 reads in 150.261 CPU sec, 4.859 real sec [M::mem_process_seqs] Processed 693502 reads in 96.696 CPU sec, 3.083 real sec sorting block 20, #reads = 1069962 mem = 536878775 peak = 956784640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282223, 9, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 329, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (302.17, 134.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (293.10, 149.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 339, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (324.03, 218.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694226 sequences (100000011 bp) in 1.891 CPU sec, 2.031 real sec... [M::process] read 693912 sequences (100000126 bp) in 1.330 CPU sec, 1.322 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 283272, 12, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 287, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (467.66, 401.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (293.87, 149.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (579, 697, 2406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6060) [M::mem_pestat] mean and std.dev: (1266.33, 1011.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 263, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1667) [M::mem_pestat] mean and std.dev: (437.66, 346.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695092 reads in 144.970 CPU sec, 4.660 real sec [M::mem_process_seqs] Processed 695072 reads in 92.304 CPU sec, 2.943 real sec sorting block 21, #reads = 1075517 mem = 536883067 peak = 956784640 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 282886, 22, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 356, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (360.17, 172.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (296.44, 150.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 274, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2723) [M::mem_pestat] mean and std.dev: (653.91, 753.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3667) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 298, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (312.68, 149.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693454 sequences (100000270 bp) in 1.856 CPU sec, 1.962 real sec... [M::process] read 693164 sequences (100000235 bp) in 1.310 CPU sec, 1.310 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 283056, 21, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 281, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1936) [M::mem_pestat] mean and std.dev: (327.24, 306.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 275, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (298.66, 151.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (522, 694, 8235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23661) [M::mem_pestat] mean and std.dev: (2670.43, 3296.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 31374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 547, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651) [M::mem_pestat] mean and std.dev: (488.10, 325.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694226 reads in 142.925 CPU sec, 4.563 real sec sorting block 22, #reads = 1078606 mem = 536879199 peak = 956784640 [M::mem_process_seqs] Processed 693912 reads in 93.411 CPU sec, 2.977 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282738, 20, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 267, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (348.59, 230.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 277, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (300.04, 151.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 652, 2413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6789) [M::mem_pestat] mean and std.dev: (863.74, 964.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8977) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 379, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1927) [M::mem_pestat] mean and std.dev: (453.44, 391.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2516) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692686 sequences (100000122 bp) in 1.866 CPU sec, 1.970 real sec... [M::process] read 692122 sequences (100000038 bp) in 1.300 CPU sec, 1.304 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282512, 16, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 313, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (340.94, 176.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 279, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (301.70, 152.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (348, 464, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (427.62, 152.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 271, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (254.18, 95.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693454 reads in 139.548 CPU sec, 4.443 real sec sorting block 23, #reads = 1077864 mem = 536884696 peak = 956784640 [M::mem_process_seqs] Processed 693164 reads in 92.411 CPU sec, 2.942 real sec sorting block 24, #reads = 1076220 mem = 536879973 peak = 986374144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282169, 14, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 395, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2653) [M::mem_pestat] mean and std.dev: (580.76, 562.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 280, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (303.34, 152.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 548, 2013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5485) [M::mem_pestat] mean and std.dev: (1355.71, 1640.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7917) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 307, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1702) [M::mem_pestat] mean and std.dev: (487.40, 439.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691682 sequences (100000251 bp) in 1.919 CPU sec, 2.029 real sec... [M::process] read 691248 sequences (100000084 bp) in 1.300 CPU sec, 1.300 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 281813, 31, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 573, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2710) [M::mem_pestat] mean and std.dev: (650.58, 454.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 281, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (303.74, 152.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (330, 617, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1716) [M::mem_pestat] mean and std.dev: (548.61, 252.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 367, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1975) [M::mem_pestat] mean and std.dev: (470.21, 333.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692686 reads in 145.663 CPU sec, 4.919 real sec [M::mem_process_seqs] Processed 692122 reads in 92.770 CPU sec, 3.026 real sec sorting block 25, #reads = 1076477 mem = 536880559 peak = 986374144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282053, 34, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 291, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4731) [M::mem_pestat] mean and std.dev: (557.52, 565.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 284, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (306.80, 154.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 194, 1091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3017) [M::mem_pestat] mean and std.dev: (415.66, 468.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 300, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (271.16, 122.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690824 sequences (100000191 bp) in 1.935 CPU sec, 2.066 real sec... [M::process] read 690678 sequences (100000281 bp) in 1.300 CPU sec, 1.294 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 281597, 24, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 289, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (257.95, 150.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 286, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (307.25, 153.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1373, 1845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4193) [M::mem_pestat] mean and std.dev: (1076.65, 651.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 326, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2095) [M::mem_pestat] mean and std.dev: (479.41, 396.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691682 reads in 144.587 CPU sec, 4.688 real sec [M::mem_process_seqs] Processed 691248 reads in 91.746 CPU sec, 2.959 real sec sorting block 26, #reads = 1074446 mem = 536876417 peak = 986374144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 281990, 16, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 334, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354) [M::mem_pestat] mean and std.dev: (351.06, 263.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 287, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (308.34, 154.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (506, 771, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2585) [M::mem_pestat] mean and std.dev: (639.57, 373.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 406, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949) [M::mem_pestat] mean and std.dev: (409.54, 266.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2544) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690542 sequences (100000264 bp) in 1.963 CPU sec, 2.101 real sec... [M::process] read 690214 sequences (100000170 bp) in 1.310 CPU sec, 1.307 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281709, 33, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 293, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2679) [M::mem_pestat] mean and std.dev: (468.32, 456.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (308.76, 153.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 175, 1152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3260) [M::mem_pestat] mean and std.dev: (559.55, 551.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 247, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353) [M::mem_pestat] mean and std.dev: (391.32, 339.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1750) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690824 reads in 143.610 CPU sec, 4.630 real sec sorting block 27, #reads = 1076742 mem = 536878396 peak = 986374144 [M::mem_process_seqs] Processed 690678 reads in 94.288 CPU sec, 3.009 real sec sorting block 28, #reads = 1075983 mem = 536883500 peak = 1032171520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 281085, 17, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 314, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157) [M::mem_pestat] mean and std.dev: (340.30, 212.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (309.70, 154.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (5, 582, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1928) [M::mem_pestat] mean and std.dev: (480.50, 429.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 513, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150) [M::mem_pestat] mean and std.dev: (620.75, 590.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690346 sequences (100000035 bp) in 2.007 CPU sec, 2.128 real sec... [M::process] read 689862 sequences (100000151 bp) in 1.320 CPU sec, 1.319 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281515, 31, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 479, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2589) [M::mem_pestat] mean and std.dev: (496.31, 459.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 291, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (311.64, 154.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (315, 665, 1982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5316) [M::mem_pestat] mean and std.dev: (1082.52, 978.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 304, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.71, 75.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690542 reads in 140.923 CPU sec, 4.495 real sec [M::mem_process_seqs] Processed 690214 reads in 93.448 CPU sec, 2.986 real sec sorting block 29, #reads = 1074370 mem = 536883956 peak = 1032171520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281724, 18, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 246, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (237.03, 103.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 291, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (311.85, 155.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 316, 1774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5244) [M::mem_pestat] mean and std.dev: (1267.67, 1737.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 373, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524) [M::mem_pestat] mean and std.dev: (414.56, 314.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689666 sequences (100000253 bp) in 1.874 CPU sec, 1.975 real sec... [M::process] read 689202 sequences (100000259 bp) in 1.340 CPU sec, 1.341 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281686, 14, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 316, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2347) [M::mem_pestat] mean and std.dev: (485.17, 456.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (312.09, 154.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1056, 1085, 2248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4632) [M::mem_pestat] mean and std.dev: (1524.86, 889.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 379, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (382.12, 190.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690346 reads in 145.642 CPU sec, 4.663 real sec [M::mem_process_seqs] Processed 689862 reads in 94.359 CPU sec, 3.022 real sec sorting block 30, #reads = 1075194 mem = 536878570 peak = 1032171520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 281066, 17, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 344, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1711) [M::mem_pestat] mean and std.dev: (458.30, 333.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 294, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (313.90, 155.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (348, 1171, 2416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6552) [M::mem_pestat] mean and std.dev: (1224.94, 929.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8620) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 253, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (240.67, 81.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689902 sequences (100000087 bp) in 2.014 CPU sec, 2.135 real sec... [M::process] read 689098 sequences (100000224 bp) in 1.320 CPU sec, 1.323 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280766, 23, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 412, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (390.97, 274.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (315.12, 156.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (661, 1248, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3190) [M::mem_pestat] mean and std.dev: (1034.05, 629.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 405, 1421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3827) [M::mem_pestat] mean and std.dev: (727.25, 562.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689666 reads in 142.479 CPU sec, 4.542 real sec sorting block 31, #reads = 1074709 mem = 536877739 peak = 1032171520 [M::mem_process_seqs] Processed 689202 reads in 101.387 CPU sec, 3.235 real sec sorting block 32, #reads = 1073092 mem = 536880399 peak = 1032171520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 281460, 19, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 181, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140) [M::mem_pestat] mean and std.dev: (216.04, 140.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 284, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (303.90, 148.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1030, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2596) [M::mem_pestat] mean and std.dev: (709.13, 481.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3244) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 245, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (262.85, 143.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689682 sequences (100000126 bp) in 2.002 CPU sec, 2.090 real sec... [M::process] read 689298 sequences (100000096 bp) in 1.340 CPU sec, 1.332 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 280601, 33, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 384, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188) [M::mem_pestat] mean and std.dev: (352.72, 197.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 289, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (308.95, 150.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 817, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3109) [M::mem_pestat] mean and std.dev: (929.67, 647.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4013) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 306, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (295.70, 121.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689902 reads in 144.505 CPU sec, 4.608 real sec [M::mem_process_seqs] Processed 689098 reads in 96.310 CPU sec, 3.076 real sec sorting block 33, #reads = 1072149 mem = 536878026 peak = 1032171520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 280663, 32, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 314, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1894) [M::mem_pestat] mean and std.dev: (444.56, 428.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 287, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (306.36, 149.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (207, 627, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4308) [M::mem_pestat] mean and std.dev: (790.47, 700.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 361, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2280) [M::mem_pestat] mean and std.dev: (513.68, 413.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2985) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689740 sequences (100000148 bp) in 1.860 CPU sec, 1.959 real sec... [M::process] read 689976 sequences (100000077 bp) in 1.330 CPU sec, 1.335 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281028, 19, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 261, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (253.96, 89.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 287, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (306.87, 149.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (826, 838, 3228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8032) [M::mem_pestat] mean and std.dev: (2093.16, 1704.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 380, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1543) [M::mem_pestat] mean and std.dev: (402.98, 233.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689682 reads in 143.972 CPU sec, 4.599 real sec [M::mem_process_seqs] Processed 689298 reads in 94.797 CPU sec, 3.061 real sec sorting block 34, #reads = 1071918 mem = 536881724 peak = 1032171520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 280654, 21, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (280.81, 180.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 285, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (304.20, 147.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 1144, 1799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4773) [M::mem_pestat] mean and std.dev: (1319.38, 1214.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1917) [M::mem_pestat] mean and std.dev: (392.35, 412.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689636 sequences (100000093 bp) in 2.064 CPU sec, 2.191 real sec... [M::process] read 689674 sequences (100000251 bp) in 1.339 CPU sec, 1.341 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281016, 15, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 243, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (260.67, 164.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 285, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (305.48, 149.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (132, 510, 3155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9201) [M::mem_pestat] mean and std.dev: (1554.60, 2348.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 295, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180) [M::mem_pestat] mean and std.dev: (314.71, 148.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689740 reads in 150.614 CPU sec, 4.827 real sec sorting block 35, #reads = 1072441 mem = 536880025 peak = 1032171520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281307, 22, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 262, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1391) [M::mem_pestat] mean and std.dev: (326.75, 247.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 689834 sequences (100000232 bp) in 1.492 CPU sec, 1.492 real sec... [M::mem_pestat] (25, 50, 75) percentile: (186, 286, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (306.43, 150.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 431, 3222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9264) [M::mem_pestat] mean and std.dev: (1810.77, 2491.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 379, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270) [M::mem_pestat] mean and std.dev: (403.61, 194.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1593) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689636 reads in 81.013 CPU sec, 2.576 real sec [M::mem_process_seqs] Processed 689976 reads in 202.175 CPU sec, 6.442 real sec sorting block 36, #reads = 1072523 mem = 536881039 peak = 1032171520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281579, 16, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 308, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (273.24, 136.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 286, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (306.65, 150.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (508, 927, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3121) [M::mem_pestat] mean and std.dev: (897.73, 423.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3992) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 300, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1342) [M::mem_pestat] mean and std.dev: (310.44, 218.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690070 sequences (100000149 bp) in 2.039 CPU sec, 2.143 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 280889, 23, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 202, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (267.58, 155.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 284, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (304.26, 148.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1444, 7592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21542) [M::mem_pestat] mean and std.dev: (3163.43, 3245.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 336, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (357.60, 208.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689844 sequences (100000249 bp) in 1.320 CPU sec, 1.317 real sec... [M::mem_process_seqs] Processed 689674 reads in 161.273 CPU sec, 5.131 real sec [M::mem_process_seqs] Processed 689834 reads in 96.091 CPU sec, 3.048 real sec sorting block 37, #reads = 1072106 mem = 536878457 peak = 1032171520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281338, 27, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 301, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2923) [M::mem_pestat] mean and std.dev: (432.03, 466.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (305.98, 150.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (463, 683, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2878) [M::mem_pestat] mean and std.dev: (811.80, 557.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3683) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 325, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1588) [M::mem_pestat] mean and std.dev: (344.26, 228.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689690 sequences (100000063 bp) in 2.078 CPU sec, 2.244 real sec... [M::process] read 689618 sequences (100000029 bp) in 1.320 CPU sec, 1.323 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281152, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 253, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482) [M::mem_pestat] mean and std.dev: (328.55, 249.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 286, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (306.10, 150.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 255, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1464) [M::mem_pestat] mean and std.dev: (351.63, 230.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690070 reads in 148.642 CPU sec, 4.980 real sec sorting block 38, #reads = 1072607 mem = 536879970 peak = 1032171520 [M::mem_process_seqs] Processed 689844 reads in 94.887 CPU sec, 3.058 real sec sorting block 39, #reads = 1071148 mem = 536880627 peak = 1097551872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281227, 25, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 354, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3134) [M::mem_pestat] mean and std.dev: (595.60, 645.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 288, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (309.07, 152.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (525, 742, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472) [M::mem_pestat] mean and std.dev: (711.59, 388.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3121) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 366, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1706) [M::mem_pestat] mean and std.dev: (471.33, 383.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2203) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689830 sequences (100000168 bp) in 1.834 CPU sec, 1.928 real sec... [M::process] read 695414 sequences (100000046 bp) in 1.360 CPU sec, 1.353 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281020, 14, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 453, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3463) [M::mem_pestat] mean and std.dev: (478.97, 398.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (309.30, 152.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 725, 3167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8725) [M::mem_pestat] mean and std.dev: (1581.21, 1608.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11504) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 298, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (288.93, 142.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689690 reads in 150.418 CPU sec, 4.834 real sec [M::mem_process_seqs] Processed 689618 reads in 97.206 CPU sec, 3.088 real sec sorting block 40, #reads = 1071881 mem = 536880601 peak = 1097551872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281428, 19, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 236, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (243.39, 148.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 289, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (309.65, 152.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (577, 863, 2691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6919) [M::mem_pestat] mean and std.dev: (1282.68, 1016.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 271, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (292.71, 161.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696150 sequences (100000282 bp) in 2.008 CPU sec, 2.139 real sec... [M::process] read 696218 sequences (100000162 bp) in 1.370 CPU sec, 1.368 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 281934, 28, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 263, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1966) [M::mem_pestat] mean and std.dev: (365.52, 296.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (291.40, 148.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 394, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2717) [M::mem_pestat] mean and std.dev: (441.96, 432.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 280, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2019) [M::mem_pestat] mean and std.dev: (286.15, 174.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689830 reads in 153.114 CPU sec, 4.922 real sec sorting block 41, #reads = 1070140 mem = 536880591 peak = 1097551872 [M::process] read 695748 sequences (100000206 bp) in 1.610 CPU sec, 1.610 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283359, 24, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 315, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2583) [M::mem_pestat] mean and std.dev: (517.26, 584.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (289.40, 147.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (103, 976, 1361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3877) [M::mem_pestat] mean and std.dev: (925.33, 910.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 334, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (387.88, 272.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696150 reads in 79.017 CPU sec, 2.623 real sec [M::mem_process_seqs] Processed 695414 reads in 202.740 CPU sec, 6.564 real sec sorting block 42, #reads = 1075385 mem = 536882593 peak = 1097551872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 283488, 23, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 287, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 570) [M::mem_pestat] mean and std.dev: (286.45, 98.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 266, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (290.86, 148.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 715, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2255) [M::mem_pestat] mean and std.dev: (741.82, 478.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2882) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 254, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (295.58, 139.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695114 sequences (100000166 bp) in 1.968 CPU sec, 2.131 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 282343, 16, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (277.82, 118.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (291.30, 148.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 598, 2802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7210) [M::mem_pestat] mean and std.dev: (1173.47, 952.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 297, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (371.61, 269.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694770 sequences (100000206 bp) in 1.349 CPU sec, 1.343 real sec... [M::mem_process_seqs] Processed 696218 reads in 163.495 CPU sec, 5.250 real sec [M::mem_process_seqs] Processed 695748 reads in 95.396 CPU sec, 3.106 real sec sorting block 43, #reads = 1076880 mem = 536880187 peak = 1097551872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 283534, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 359, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2021) [M::mem_pestat] mean and std.dev: (453.56, 342.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (292.69, 148.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 264, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (411.58, 299.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1611) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694466 sequences (100000213 bp) in 2.439 CPU sec, 2.612 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 283429, 17, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 289, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (250.79, 102.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (295.02, 150.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (320, 935, 2487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6821) [M::mem_pestat] mean and std.dev: (1402.19, 1532.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 325, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3102) [M::mem_pestat] mean and std.dev: (643.18, 749.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4073) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693534 sequences (100000184 bp) in 1.410 CPU sec, 1.409 real sec... [M::mem_process_seqs] Processed 695114 reads in 146.750 CPU sec, 4.752 real sec [M::mem_process_seqs] Processed 694770 reads in 94.120 CPU sec, 2.999 real sec sorting block 44, #reads = 1076805 mem = 536878978 peak = 1097551872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 283006, 23, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 227, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (236.95, 126.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (294.88, 149.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (511, 628, 1889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4645) [M::mem_pestat] mean and std.dev: (839.67, 599.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 288, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1825) [M::mem_pestat] mean and std.dev: (444.58, 381.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 45, #reads = 1077108 mem = 536877785 peak = 1097551872 [M::process] read 693014 sequences (100000069 bp) in 1.998 CPU sec, 2.118 real sec... [M::process] read 692938 sequences (100000111 bp) in 1.330 CPU sec, 1.337 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 282672, 13, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 310, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1374) [M::mem_pestat] mean and std.dev: (414.84, 323.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 274, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (298.74, 151.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 630, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3297) [M::mem_pestat] mean and std.dev: (808.83, 626.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 366, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449) [M::mem_pestat] mean and std.dev: (344.03, 200.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694466 reads in 144.156 CPU sec, 4.590 real sec [M::mem_process_seqs] Processed 693534 reads in 94.682 CPU sec, 3.009 real sec sorting block 46, #reads = 1075830 mem = 536881796 peak = 1097551872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282071, 14, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 263, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (325.24, 221.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 278, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (301.64, 152.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (319, 458, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (407.64, 104.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 387, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486) [M::mem_pestat] mean and std.dev: (398.51, 207.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692586 sequences (100000252 bp) in 2.104 CPU sec, 2.251 real sec... [M::process] read 692188 sequences (100000183 bp) in 1.440 CPU sec, 1.435 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282776, 17, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 337, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348) [M::mem_pestat] mean and std.dev: (371.03, 266.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 278, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (301.70, 152.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (407, 771, 1530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3776) [M::mem_pestat] mean and std.dev: (664.60, 503.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 450, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192) [M::mem_pestat] mean and std.dev: (509.82, 387.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693014 reads in 146.224 CPU sec, 4.889 real sec [M::mem_process_seqs] Processed 692938 reads in 93.496 CPU sec, 2.998 real sec sorting block 47, #reads = 1075837 mem = 536878038 peak = 1097551872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 282300, 16, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 333, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (355.45, 204.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 279, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (302.44, 152.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 1079, 2011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5563) [M::mem_pestat] mean and std.dev: (1051.50, 1034.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7339) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 291, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1566) [M::mem_pestat] mean and std.dev: (349.19, 211.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691430 sequences (100000221 bp) in 2.270 CPU sec, 2.394 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 282353, 15, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 293, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1460) [M::mem_pestat] mean and std.dev: (359.89, 214.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 281, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (304.67, 153.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (539, 690, 2488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6386) [M::mem_pestat] mean and std.dev: (923.54, 908.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 372, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882) [M::mem_pestat] mean and std.dev: (398.31, 285.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691294 sequences (100000193 bp) in 1.450 CPU sec, 1.448 real sec... [M::mem_process_seqs] Processed 692586 reads in 146.756 CPU sec, 4.702 real sec [M::mem_process_seqs] Processed 692188 reads in 93.875 CPU sec, 3.061 real sec sorting block 48, #reads = 1076276 mem = 536879295 peak = 1097551872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 282018, 15, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 320, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515) [M::mem_pestat] mean and std.dev: (418.30, 313.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 284, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (306.65, 153.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (598, 1299, 1974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4726) [M::mem_pestat] mean and std.dev: (1306.00, 1069.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 270, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1958) [M::mem_pestat] mean and std.dev: (465.69, 376.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691618 sequences (100000133 bp) in 1.977 CPU sec, 2.082 real sec... [M::process] read 691228 sequences (100000009 bp) in 1.310 CPU sec, 1.311 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 282555, 25, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 291, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3438) [M::mem_pestat] mean and std.dev: (654.81, 676.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 286, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (308.75, 155.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (513, 1060, 9688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28038) [M::mem_pestat] mean and std.dev: (3644.68, 4111.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 37213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 340, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1266) [M::mem_pestat] mean and std.dev: (350.12, 216.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691430 reads in 146.497 CPU sec, 4.759 real sec sorting block 49, #reads = 1075298 mem = 536878326 peak = 1097551872 [M::mem_process_seqs] Processed 691294 reads in 92.929 CPU sec, 2.964 real sec sorting block 50, #reads = 1075679 mem = 536878751 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 281516, 3, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 448, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2602) [M::mem_pestat] mean and std.dev: (514.17, 381.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (307.46, 155.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 352, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (308.09, 154.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690876 sequences (100000142 bp) in 2.090 CPU sec, 2.238 real sec... [M::process] read 690762 sequences (100000260 bp) in 1.320 CPU sec, 1.321 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282157, 9, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 347, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (304.71, 155.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 287, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (309.40, 155.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 234, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (232.18, 87.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691618 reads in 146.791 CPU sec, 4.679 real sec [M::mem_process_seqs] Processed 691228 reads in 94.482 CPU sec, 2.997 real sec sorting block 51, #reads = 1074382 mem = 536879493 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 281857, 41, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 325, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246) [M::mem_pestat] mean and std.dev: (443.68, 289.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 287, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (310.30, 155.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (254, 470, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1964) [M::mem_pestat] mean and std.dev: (486.28, 401.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2534) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 406, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1843) [M::mem_pestat] mean and std.dev: (402.24, 229.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691394 sequences (100000166 bp) in 1.968 CPU sec, 2.075 real sec... [M::process] read 691676 sequences (100000167 bp) in 1.320 CPU sec, 1.322 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 281527, 25, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 268, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (274.26, 113.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (311.02, 156.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (871, 1649, 3494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8740) [M::mem_pestat] mean and std.dev: (2008.64, 1588.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 239, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (293.84, 201.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690876 reads in 147.455 CPU sec, 4.741 real sec [M::mem_process_seqs] Processed 690762 reads in 93.865 CPU sec, 2.983 real sec sorting block 52, #reads = 1074031 mem = 536880069 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281813, 23, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 307, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2664) [M::mem_pestat] mean and std.dev: (524.79, 532.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (304.03, 151.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1200, 1298, 2005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3615) [M::mem_pestat] mean and std.dev: (1347.75, 733.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4420) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 445, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570) [M::mem_pestat] mean and std.dev: (478.68, 359.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691658 sequences (100000011 bp) in 2.065 CPU sec, 2.190 real sec... [M::process] read 690848 sequences (100000070 bp) in 1.320 CPU sec, 1.317 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281617, 21, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 262, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (290.76, 161.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 276, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (299.31, 149.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1476, 2008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4802) [M::mem_pestat] mean and std.dev: (1306.15, 637.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 372, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (349.48, 223.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691394 reads in 144.083 CPU sec, 4.600 real sec sorting block 53, #reads = 1074445 mem = 536879039 peak = 1130930176 [M::mem_process_seqs] Processed 691676 reads in 94.893 CPU sec, 3.100 real sec sorting block 54, #reads = 1074476 mem = 536879813 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281470, 18, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 292, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1307) [M::mem_pestat] mean and std.dev: (311.56, 196.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 279, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (301.62, 150.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (368, 462, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2378) [M::mem_pestat] mean and std.dev: (568.19, 450.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3048) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 244, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1824) [M::mem_pestat] mean and std.dev: (363.59, 317.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689472 sequences (100000001 bp) in 1.900 CPU sec, 1.997 real sec... [M::process] read 689500 sequences (100000093 bp) in 1.320 CPU sec, 1.315 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281546, 11, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 296, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (320.21, 215.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (306.20, 152.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 784, 1771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3983) [M::mem_pestat] mean and std.dev: (982.70, 766.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5089) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 340, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138) [M::mem_pestat] mean and std.dev: (517.38, 477.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691658 reads in 149.317 CPU sec, 4.832 real sec [M::mem_process_seqs] Processed 690848 reads in 94.824 CPU sec, 3.038 real sec sorting block 55, #reads = 1073687 mem = 536877772 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280880, 17, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 324, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785) [M::mem_pestat] mean and std.dev: (447.83, 379.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 289, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (309.86, 152.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 208, 2382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7022) [M::mem_pestat] mean and std.dev: (1298.76, 1816.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 310, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (275.34, 142.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689844 sequences (100000010 bp) in 2.002 CPU sec, 2.098 real sec... [M::process] read 689720 sequences (100000139 bp) in 1.320 CPU sec, 1.329 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280790, 20, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (161.00, 68.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 288, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (309.22, 152.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (580, 934, 2213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5479) [M::mem_pestat] mean and std.dev: (1263.25, 1032.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 405, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175) [M::mem_pestat] mean and std.dev: (539.79, 420.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689472 reads in 148.762 CPU sec, 4.773 real sec [M::mem_process_seqs] Processed 689500 reads in 95.097 CPU sec, 3.094 real sec sorting block 56, #reads = 1072729 mem = 536878966 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281096, 20, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 328, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218) [M::mem_pestat] mean and std.dev: (396.90, 257.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 288, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (308.74, 152.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (746, 837, 5164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14000) [M::mem_pestat] mean and std.dev: (2268.75, 2919.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18418) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 400, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507) [M::mem_pestat] mean and std.dev: (395.86, 235.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689710 sequences (100000052 bp) in 1.863 CPU sec, 1.958 real sec... [M::process] read 689754 sequences (100000016 bp) in 1.340 CPU sec, 1.334 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281065, 18, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 499, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1962) [M::mem_pestat] mean and std.dev: (469.80, 342.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 289, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (310.54, 154.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (396, 868, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2760) [M::mem_pestat] mean and std.dev: (806.29, 502.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 350, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (458.85, 345.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689844 reads in 149.447 CPU sec, 4.830 real sec sorting block 57, #reads = 1072272 mem = 536878007 peak = 1130930176 [M::mem_process_seqs] Processed 689720 reads in 95.690 CPU sec, 3.145 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281392, 19, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 381, 1257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3447) [M::mem_pestat] mean and std.dev: (490.48, 472.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 288, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (309.61, 153.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (440, 762, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3137) [M::mem_pestat] mean and std.dev: (885.68, 643.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 359, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2938) [M::mem_pestat] mean and std.dev: (728.31, 817.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689628 sequences (100000283 bp) in 1.974 CPU sec, 2.061 real sec... [M::process] read 690662 sequences (100000120 bp) in 1.370 CPU sec, 1.372 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281402, 15, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 314, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (349.45, 219.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 288, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (309.02, 153.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 678, 2361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6701) [M::mem_pestat] mean and std.dev: (1252.00, 1565.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8871) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 632, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761) [M::mem_pestat] mean and std.dev: (540.75, 365.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689710 reads in 149.168 CPU sec, 4.842 real sec sorting block 58, #reads = 1072394 mem = 536885135 peak = 1130930176 [M::mem_process_seqs] Processed 689754 reads in 97.465 CPU sec, 3.109 real sec sorting block 59, #reads = 1071637 mem = 536880224 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280963, 22, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 275, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (276.89, 181.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (309.34, 152.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (491, 1389, 4064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11210) [M::mem_pestat] mean and std.dev: (2110.55, 1747.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14783) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 362, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (374.29, 202.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689716 sequences (100000020 bp) in 1.998 CPU sec, 2.099 real sec... [M::process] read 688804 sequences (100000013 bp) in 1.330 CPU sec, 1.337 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 281754, 20, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 232, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (222.52, 65.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (306.17, 152.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1077, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3388) [M::mem_pestat] mean and std.dev: (1074.70, 660.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 428, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344) [M::mem_pestat] mean and std.dev: (418.88, 244.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689628 reads in 147.411 CPU sec, 4.702 real sec [M::mem_process_seqs] Processed 690662 reads in 98.772 CPU sec, 3.205 real sec sorting block 60, #reads = 1073095 mem = 536879380 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281285, 19, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 227, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (260.43, 135.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (311.25, 154.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (596, 634, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1490) [M::mem_pestat] mean and std.dev: (763.58, 351.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2168) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 292, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2768) [M::mem_pestat] mean and std.dev: (512.19, 492.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693112 sequences (100000136 bp) in 1.959 CPU sec, 2.034 real sec... [M::process] read 695802 sequences (100000154 bp) in 1.320 CPU sec, 1.321 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280797, 20, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 436, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4158) [M::mem_pestat] mean and std.dev: (793.73, 767.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 294, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (315.67, 157.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 587, 1906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5248) [M::mem_pestat] mean and std.dev: (883.75, 776.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6919) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 331, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (386.79, 271.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689716 reads in 150.021 CPU sec, 4.843 real sec sorting block 61, #reads = 1071893 mem = 536877193 peak = 1130930176 [M::process] read 696000 sequences (100000006 bp) in 1.493 CPU sec, 1.498 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281884, 24, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 237, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2332) [M::mem_pestat] mean and std.dev: (457.11, 461.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (298.63, 150.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (441, 814, 2832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7614) [M::mem_pestat] mean and std.dev: (1464.88, 1404.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10005) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 404, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729) [M::mem_pestat] mean and std.dev: (426.27, 277.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693112 reads in 83.127 CPU sec, 2.643 real sec [M::mem_process_seqs] Processed 688804 reads in 205.620 CPU sec, 6.558 real sec sorting block 62, #reads = 1072273 mem = 536878536 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 283171, 18, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038) [M::mem_pestat] mean and std.dev: (428.44, 425.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (290.65, 148.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (513, 1695, 5894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16656) [M::mem_pestat] mean and std.dev: (3034.61, 3144.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 296, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (299.06, 172.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695644 sequences (100000251 bp) in 2.133 CPU sec, 2.266 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283037, 24, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 300, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2041) [M::mem_pestat] mean and std.dev: (478.43, 448.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (290.63, 148.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 1471, 1764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4692) [M::mem_pestat] mean and std.dev: (909.62, 736.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6156) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 240, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (256.00, 115.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695126 sequences (100000228 bp) in 1.290 CPU sec, 1.296 real sec... [M::mem_process_seqs] Processed 695802 reads in 157.931 CPU sec, 5.030 real sec [M::mem_process_seqs] Processed 696000 reads in 101.133 CPU sec, 3.221 real sec sorting block 63, #reads = 1074860 mem = 536878954 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282960, 24, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 217, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (206.59, 102.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (291.98, 149.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 1399, 3704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10758) [M::mem_pestat] mean and std.dev: (1736.46, 1841.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 468, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2792) [M::mem_pestat] mean and std.dev: (597.05, 427.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694730 sequences (100000061 bp) in 2.106 CPU sec, 2.304 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282798, 24, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 348, 1327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3513) [M::mem_pestat] mean and std.dev: (609.60, 516.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (291.43, 147.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 695, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7305) [M::mem_pestat] mean and std.dev: (1824.88, 2478.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11738) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 445, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330) [M::mem_pestat] mean and std.dev: (435.94, 236.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694340 sequences (100000226 bp) in 1.290 CPU sec, 1.293 real sec... [M::mem_process_seqs] Processed 695644 reads in 140.475 CPU sec, 4.478 real sec sorting block 64, #reads = 1079218 mem = 536884465 peak = 1130930176 [M::mem_process_seqs] Processed 695126 reads in 92.519 CPU sec, 2.973 real sec sorting block 65, #reads = 1078282 mem = 536878797 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283135, 15, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 234, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (295.86, 201.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.75, 148.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (173, 636, 1756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4922) [M::mem_pestat] mean and std.dev: (1125.00, 1477.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7034) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 382, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2113) [M::mem_pestat] mean and std.dev: (384.32, 255.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694756 sequences (100000262 bp) in 1.956 CPU sec, 2.064 real sec... [M::process] read 693422 sequences (100000134 bp) in 1.320 CPU sec, 1.311 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282874, 9, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 232, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (275.02, 202.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (294.78, 150.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 220, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2967) [M::mem_pestat] mean and std.dev: (436.79, 475.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694730 reads in 145.349 CPU sec, 4.678 real sec [M::mem_process_seqs] Processed 694340 reads in 94.007 CPU sec, 2.998 real sec sorting block 66, #reads = 1077401 mem = 536878731 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282954, 10, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 273, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (286.36, 136.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (294.52, 150.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1059, 1059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1833) [M::mem_pestat] mean and std.dev: (885.56, 401.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2493) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 321, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (322.10, 195.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693306 sequences (100000206 bp) in 1.920 CPU sec, 2.023 real sec... [M::process] read 693274 sequences (100000197 bp) in 1.350 CPU sec, 1.356 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282446, 14, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 303, 1020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2578) [M::mem_pestat] mean and std.dev: (510.94, 444.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (298.57, 151.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 951, 2070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5730) [M::mem_pestat] mean and std.dev: (1058.00, 1085.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7560) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 366, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (388.38, 250.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694756 reads in 140.314 CPU sec, 4.484 real sec sorting block 67, #reads = 1078482 mem = 536882584 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282174, 23, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 301, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1677) [M::mem_pestat] mean and std.dev: (366.00, 238.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (298.67, 151.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 1135, 1961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4703) [M::mem_pestat] mean and std.dev: (1143.23, 671.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6074) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 385, 726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1694) [M::mem_pestat] mean and std.dev: (392.74, 264.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692904 sequences (100000009 bp) in 1.765 CPU sec, 1.768 real sec... [M::mem_process_seqs] Processed 693306 reads in 83.280 CPU sec, 2.881 real sec [M::mem_process_seqs] Processed 693422 reads in 202.392 CPU sec, 6.689 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282603, 28, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 227, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (271.97, 136.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 275, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (298.83, 151.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (566, 935, 2139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5285) [M::mem_pestat] mean and std.dev: (1234.25, 892.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6858) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 570, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1717) [M::mem_pestat] mean and std.dev: (493.87, 311.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 68, #reads = 1077226 mem = 536879447 peak = 1130930176 [M::process] read 692884 sequences (100000270 bp) in 2.237 CPU sec, 2.528 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 282442, 31, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 277, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (322.61, 174.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 278, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (301.53, 152.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (742, 742, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1984) [M::mem_pestat] mean and std.dev: (832.76, 274.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 279, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1823) [M::mem_pestat] mean and std.dev: (492.28, 430.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693274 reads in 156.843 CPU sec, 4.987 real sec [M::mem_process_seqs] Processed 692904 reads in 94.614 CPU sec, 2.991 real sec [M::process] read 691928 sequences (100000121 bp) in 1.663 CPU sec, 1.723 real sec... sorting block 69, #reads = 1078013 mem = 536878631 peak = 1130930176 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282000, 25, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 252, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (241.00, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 279, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (303.32, 154.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 471, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3365) [M::mem_pestat] mean and std.dev: (765.83, 1038.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4918) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 305, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2575) [M::mem_pestat] mean and std.dev: (524.61, 551.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692214 sequences (100000105 bp) in 2.143 CPU sec, 2.297 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281297, 28, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 309, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (302.86, 108.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (303.60, 153.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (578, 956, 2040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4964) [M::mem_pestat] mean and std.dev: (1125.85, 905.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 390, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853) [M::mem_pestat] mean and std.dev: (462.58, 348.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692058 sequences (100000045 bp) in 1.420 CPU sec, 1.417 real sec... [M::mem_process_seqs] Processed 692884 reads in 142.478 CPU sec, 4.537 real sec sorting block 70, #reads = 1076100 mem = 536879163 peak = 1130930176 [M::mem_process_seqs] Processed 691928 reads in 93.792 CPU sec, 3.175 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281928, 10, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 305, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (343.79, 184.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 282, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (305.63, 155.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1224, 6985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19595) [M::mem_pestat] mean and std.dev: (2674.90, 2838.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25900) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 308, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372) [M::mem_pestat] mean and std.dev: (325.22, 177.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691652 sequences (100000004 bp) in 2.318 CPU sec, 2.476 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282004, 15, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 272, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2882) [M::mem_pestat] mean and std.dev: (577.16, 542.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 283, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (306.45, 155.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (569, 806, 2874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7484) [M::mem_pestat] mean and std.dev: (1440.60, 1302.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 358, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (385.65, 207.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691256 sequences (100000011 bp) in 1.450 CPU sec, 1.447 real sec... [M::mem_process_seqs] Processed 692214 reads in 145.955 CPU sec, 4.833 real sec sorting block 71, #reads = 1075664 mem = 536879237 peak = 1130930176 [M::mem_process_seqs] Processed 692058 reads in 94.197 CPU sec, 3.019 real sec sorting block 72, #reads = 1075883 mem = 536877781 peak = 1164963840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281934, 18, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 279, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3403) [M::mem_pestat] mean and std.dev: (555.66, 683.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 284, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (307.92, 155.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 457, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593) [M::mem_pestat] mean and std.dev: (470.38, 467.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 422, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3542) [M::mem_pestat] mean and std.dev: (887.82, 1015.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690970 sequences (100000027 bp) in 2.268 CPU sec, 2.404 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281469, 24, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 309, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2645) [M::mem_pestat] mean and std.dev: (520.00, 545.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 286, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (308.95, 155.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (290, 994, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2972) [M::mem_pestat] mean and std.dev: (716.29, 528.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 378, 1432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3856) [M::mem_pestat] mean and std.dev: (717.98, 773.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690980 sequences (100000207 bp) in 1.440 CPU sec, 1.439 real sec... [M::mem_process_seqs] Processed 691652 reads in 146.692 CPU sec, 4.707 real sec [M::mem_process_seqs] Processed 691256 reads in 96.906 CPU sec, 3.094 real sec sorting block 73, #reads = 1075923 mem = 536882056 peak = 1164963840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281346, 19, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 229, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (284.45, 194.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 286, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (308.18, 154.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (587, 700, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (173, 1208) [M::mem_pestat] mean and std.dev: (696.50, 130.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 341, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (327.81, 144.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691078 sequences (100000262 bp) in 2.326 CPU sec, 2.433 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281218, 17, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 230, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (262.72, 135.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 288, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (309.41, 155.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (568, 901, 3250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8614) [M::mem_pestat] mean and std.dev: (1655.94, 1819.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 304, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (313.72, 164.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690238 sequences (100000282 bp) in 1.460 CPU sec, 1.460 real sec... [M::mem_process_seqs] Processed 690970 reads in 144.257 CPU sec, 4.584 real sec [M::mem_process_seqs] Processed 690980 reads in 95.311 CPU sec, 3.022 real sec sorting block 74, #reads = 1074575 mem = 536878201 peak = 1164963840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281451, 24, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 290, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (343.51, 227.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 286, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (307.43, 153.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 838, 1670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4436) [M::mem_pestat] mean and std.dev: (1118.71, 1185.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 329, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (292.00, 107.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689744 sequences (100000283 bp) in 2.163 CPU sec, 2.258 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 281281, 17, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 351, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1566) [M::mem_pestat] mean and std.dev: (437.98, 316.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (309.17, 152.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (874, 1465, 1915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3997) [M::mem_pestat] mean and std.dev: (1414.88, 873.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 364, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (334.35, 190.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689776 sequences (100000139 bp) in 1.420 CPU sec, 1.425 real sec... [M::mem_process_seqs] Processed 691078 reads in 149.347 CPU sec, 4.808 real sec sorting block 75, #reads = 1074790 mem = 536878580 peak = 1164963840 [M::mem_process_seqs] Processed 690238 reads in 95.952 CPU sec, 3.043 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 280718, 13, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 327, 1361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3495) [M::mem_pestat] mean and std.dev: (579.26, 561.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (310.89, 153.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (577, 838, 1794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4228) [M::mem_pestat] mean and std.dev: (997.31, 598.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 298, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (312.68, 180.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 76, #reads = 1073125 mem = 536879127 peak = 1198346240 [M::process] read 689644 sequences (100000009 bp) in 2.308 CPU sec, 2.414 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 281041, 25, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 278, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3150) [M::mem_pestat] mean and std.dev: (372.55, 361.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (311.37, 153.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 549, 1066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2528) [M::mem_pestat] mean and std.dev: (801.96, 784.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3939) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 275, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (284.91, 108.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688994 sequences (100000057 bp) in 1.460 CPU sec, 1.454 real sec... [M::mem_process_seqs] Processed 689744 reads in 145.506 CPU sec, 4.628 real sec [M::mem_process_seqs] Processed 689776 reads in 96.271 CPU sec, 3.053 real sec sorting block 77, #reads = 1073028 mem = 536881687 peak = 1198346240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 281055, 17, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 368, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (319.69, 157.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (311.03, 152.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 510, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3056) [M::mem_pestat] mean and std.dev: (555.20, 672.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4055) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 243, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538) [M::mem_pestat] mean and std.dev: (300.05, 223.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689018 sequences (100000167 bp) in 2.384 CPU sec, 2.516 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 280849, 32, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 271, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (298.34, 151.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 291, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (311.61, 152.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 677, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3025) [M::mem_pestat] mean and std.dev: (638.32, 537.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 282, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1409) [M::mem_pestat] mean and std.dev: (340.82, 200.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688978 sequences (100000207 bp) in 1.410 CPU sec, 1.412 real sec... [M::mem_process_seqs] Processed 689644 reads in 149.087 CPU sec, 5.002 real sec sorting block 78, #reads = 1072356 mem = 536878974 peak = 1198346240 [M::process] read 689064 sequences (100000144 bp) in 1.473 CPU sec, 1.479 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 280673, 19, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 305, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2035) [M::mem_pestat] mean and std.dev: (418.22, 365.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 292, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (311.66, 152.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (461, 970, 1507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3599) [M::mem_pestat] mean and std.dev: (988.06, 872.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 357, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (368.23, 207.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689018 reads in 79.912 CPU sec, 2.599 real sec [M::mem_process_seqs] Processed 688994 reads in 198.462 CPU sec, 6.378 real sec sorting block 79, #reads = 1072089 mem = 536883537 peak = 1198346240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 280792, 8, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 403, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191) [M::mem_pestat] mean and std.dev: (424.38, 249.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 292, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (311.47, 152.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 327, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2285) [M::mem_pestat] mean and std.dev: (452.67, 373.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689082 sequences (100000132 bp) in 1.882 CPU sec, 2.144 real sec... [M::process] read 688978 sequences (100000104 bp) in 1.310 CPU sec, 1.311 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281062, 31, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 247, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (311.90, 244.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 291, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (310.86, 152.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (304, 479, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3646) [M::mem_pestat] mean and std.dev: (816.67, 769.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (340, 423, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005) [M::mem_pestat] mean and std.dev: (557.53, 370.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688978 reads in 164.891 CPU sec, 5.292 real sec [M::mem_process_seqs] Processed 689064 reads in 98.753 CPU sec, 3.156 real sec sorting block 80, #reads = 1072565 mem = 536880020 peak = 1198346240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 280166, 19, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 315, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (315.22, 180.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 289, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (309.18, 151.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (514, 780, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2872) [M::mem_pestat] mean and std.dev: (1003.44, 759.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4042) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 258, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (285.84, 150.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689028 sequences (100000230 bp) in 2.127 CPU sec, 2.286 real sec... [M::process] read 688904 sequences (100000281 bp) in 1.320 CPU sec, 1.319 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280862, 15, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 304, 991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2621) [M::mem_pestat] mean and std.dev: (452.06, 479.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 289, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (308.78, 150.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 829, 3978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10660) [M::mem_pestat] mean and std.dev: (1929.27, 2190.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14001) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 344, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (332.35, 198.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689082 reads in 146.844 CPU sec, 4.672 real sec sorting block 81, #reads = 1071325 mem = 536884909 peak = 1198346240 [M::mem_process_seqs] Processed 688978 reads in 97.012 CPU sec, 3.088 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281102, 19, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 263, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (272.71, 154.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 290, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (309.18, 150.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 878, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5302) [M::mem_pestat] mean and std.dev: (1108.16, 886.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7013) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 426, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425) [M::mem_pestat] mean and std.dev: (380.37, 238.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696986 sequences (100000190 bp) in 2.249 CPU sec, 2.376 real sec... [M::process] read 700068 sequences (100000014 bp) in 1.380 CPU sec, 1.379 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280755, 11, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 253, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1719) [M::mem_pestat] mean and std.dev: (369.26, 396.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 291, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (310.32, 151.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (411, 1943, 5814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16620) [M::mem_pestat] mean and std.dev: (2719.27, 2804.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 316, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (327.17, 178.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689028 reads in 146.571 CPU sec, 4.666 real sec sorting block 82, #reads = 1072097 mem = 536880900 peak = 1198346240 [M::mem_process_seqs] Processed 688904 reads in 98.532 CPU sec, 3.142 real sec sorting block 83, #reads = 1070409 mem = 536880493 peak = 1216245760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282891, 25, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 309, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (347.87, 216.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (284.24, 145.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 824, 2025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5491) [M::mem_pestat] mean and std.dev: (1201.12, 1186.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 352, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1782) [M::mem_pestat] mean and std.dev: (510.05, 398.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698712 sequences (100000172 bp) in 1.940 CPU sec, 2.036 real sec... [M::process] read 698568 sequences (100000262 bp) in 1.380 CPU sec, 1.378 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285099, 13, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 244, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (264.00, 171.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (278.35, 146.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (363, 727, 2276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6102) [M::mem_pestat] mean and std.dev: (1301.46, 1569.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8015) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 477, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3358) [M::mem_pestat] mean and std.dev: (734.37, 741.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4413) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696986 reads in 161.822 CPU sec, 5.158 real sec [M::mem_process_seqs] Processed 700068 reads in 104.411 CPU sec, 3.332 real sec sorting block 84, #reads = 1069052 mem = 536882585 peak = 1216245760 [M::process] read 697904 sequences (100000035 bp) in 1.992 CPU sec, 2.086 real sec... [M::process] read 695442 sequences (100000289 bp) in 1.360 CPU sec, 1.354 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284408, 18, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 224, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2930) [M::mem_pestat] mean and std.dev: (531.07, 573.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 253, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (283.58, 148.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 578, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3973) [M::mem_pestat] mean and std.dev: (603.19, 591.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 360, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1135) [M::mem_pestat] mean and std.dev: (395.27, 238.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698568 reads in 90.451 CPU sec, 2.863 real sec sorting block 85, #reads = 1074776 mem = 536877189 peak = 1216245760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284775, 13, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 278, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (324.60, 201.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (282.14, 147.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 694, 4899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14159) [M::mem_pestat] mean and std.dev: (2196.23, 3141.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 305, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2139) [M::mem_pestat] mean and std.dev: (489.59, 423.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284345, 17, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 330, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217) [M::mem_pestat] mean and std.dev: (330.84, 214.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (284.93, 148.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 532, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2335) [M::mem_pestat] mean and std.dev: (494.38, 394.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3034) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 334, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2662) [M::mem_pestat] mean and std.dev: (502.76, 414.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695000 sequences (100000288 bp) in 1.473 CPU sec, 1.477 real sec... [M::mem_process_seqs] Processed 698712 reads in 242.683 CPU sec, 7.733 real sec [M::mem_process_seqs] Processed 697904 reads in 119.925 CPU sec, 3.850 real sec sorting block 86, #reads = 1075267 mem = 536880722 peak = 1216245760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283431, 25, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 251, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2443) [M::mem_pestat] mean and std.dev: (460.88, 540.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (292.81, 150.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (396, 645, 2318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6162) [M::mem_pestat] mean and std.dev: (1000.05, 1052.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 324, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2090) [M::mem_pestat] mean and std.dev: (547.98, 495.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694396 sequences (100000229 bp) in 2.121 CPU sec, 2.268 real sec... [M::process] read 693858 sequences (100000090 bp) in 1.340 CPU sec, 1.334 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283634, 22, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 274, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (234.44, 93.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (295.48, 152.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (373, 1227, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12031) [M::mem_pestat] mean and std.dev: (2433.64, 2741.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15917) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 290, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (292.07, 161.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695442 reads in 152.653 CPU sec, 4.924 real sec [M::mem_process_seqs] Processed 695000 reads in 97.085 CPU sec, 3.092 real sec sorting block 87, #reads = 1074340 mem = 536879408 peak = 1216245760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283689, 15, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 245, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (275.03, 202.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (297.45, 152.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 485, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3101) [M::mem_pestat] mean and std.dev: (553.00, 611.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 263, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (347.85, 254.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693128 sequences (100000223 bp) in 2.137 CPU sec, 2.275 real sec... [M::process] read 692822 sequences (100000223 bp) in 1.360 CPU sec, 1.360 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 283087, 13, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 280, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (376.75, 238.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 274, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (300.35, 154.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (6, 293, 2111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6321) [M::mem_pestat] mean and std.dev: (889.77, 1270.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 474, 1412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3688) [M::mem_pestat] mean and std.dev: (803.11, 777.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694396 reads in 148.102 CPU sec, 4.709 real sec sorting block 88, #reads = 1075605 mem = 536883649 peak = 1216245760 [M::mem_process_seqs] Processed 693858 reads in 96.633 CPU sec, 3.075 real sec sorting block 89, #reads = 1074874 mem = 536876945 peak = 1216245760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282459, 13, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 290, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (279.13, 123.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 277, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (303.01, 154.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (795, 839, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4173) [M::mem_pestat] mean and std.dev: (992.42, 589.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 287, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (352.36, 245.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693264 sequences (100000138 bp) in 1.911 CPU sec, 2.039 real sec... [M::process] read 692120 sequences (100000156 bp) in 1.360 CPU sec, 1.365 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 282612, 14, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 409, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432) [M::mem_pestat] mean and std.dev: (369.15, 196.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 278, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (303.94, 154.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 1513, 2569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7595) [M::mem_pestat] mean and std.dev: (1448.79, 1485.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10108) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 349, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (414.74, 292.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693128 reads in 152.271 CPU sec, 4.862 real sec [M::mem_process_seqs] Processed 692822 reads in 97.001 CPU sec, 3.089 real sec sorting block 90, #reads = 1074754 mem = 536880745 peak = 1216245760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282684, 19, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 449, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1992) [M::mem_pestat] mean and std.dev: (465.38, 468.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 276, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (301.35, 153.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (926, 1642, 1642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3074) [M::mem_pestat] mean and std.dev: (1242.47, 600.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 350, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2088) [M::mem_pestat] mean and std.dev: (448.85, 360.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691928 sequences (100000060 bp) in 1.962 CPU sec, 2.104 real sec... [M::process] read 692368 sequences (100000076 bp) in 1.360 CPU sec, 1.353 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282368, 16, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 322, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712) [M::mem_pestat] mean and std.dev: (446.95, 321.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 281, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (307.03, 156.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1534, 1534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3282) [M::mem_pestat] mean and std.dev: (1185.00, 708.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4156) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 279, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558) [M::mem_pestat] mean and std.dev: (424.31, 359.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2010) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693264 reads in 155.448 CPU sec, 4.999 real sec [M::mem_process_seqs] Processed 692120 reads in 99.573 CPU sec, 3.272 real sec sorting block 91, #reads = 1073850 mem = 536879316 peak = 1216245760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282268, 18, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 275, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2773) [M::mem_pestat] mean and std.dev: (568.23, 504.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 284, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (309.65, 158.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 621, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505) [M::mem_pestat] mean and std.dev: (654.06, 509.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 302, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (299.40, 155.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691898 sequences (100000290 bp) in 1.947 CPU sec, 2.078 real sec... [M::process] read 691236 sequences (100000194 bp) in 1.360 CPU sec, 1.360 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282550, 19, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 238, 1517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4227) [M::mem_pestat] mean and std.dev: (790.03, 860.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 282, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (308.49, 158.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (566, 958, 1799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4265) [M::mem_pestat] mean and std.dev: (1034.94, 739.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 274, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1971) [M::mem_pestat] mean and std.dev: (490.50, 468.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691928 reads in 156.465 CPU sec, 5.078 real sec [M::mem_process_seqs] Processed 692368 reads in 100.080 CPU sec, 3.185 real sec sorting block 92, #reads = 1072285 mem = 536881511 peak = 1216245760 sorting block 93, #reads = 1072161 mem = 536880758 peak = 1227476992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281943, 21, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 324, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (259.88, 91.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 282, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (308.10, 157.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (84, 1142, 2913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8571) [M::mem_pestat] mean and std.dev: (797.00, 791.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 336, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (285.78, 109.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691020 sequences (100000051 bp) in 2.036 CPU sec, 2.155 real sec... [M::process] read 690480 sequences (100000117 bp) in 1.360 CPU sec, 1.360 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 281075, 24, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 358, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (328.56, 161.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (306.98, 154.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (692, 1441, 1507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3137) [M::mem_pestat] mean and std.dev: (1021.38, 532.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3952) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 281, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2307) [M::mem_pestat] mean and std.dev: (438.53, 451.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691898 reads in 157.809 CPU sec, 5.038 real sec [M::mem_process_seqs] Processed 691236 reads in 104.313 CPU sec, 3.364 real sec sorting block 94, #reads = 1070278 mem = 536878081 peak = 1227476992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281236, 9, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 240, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2348) [M::mem_pestat] mean and std.dev: (453.92, 388.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (307.76, 154.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 289, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (259.37, 122.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690372 sequences (100000230 bp) in 1.968 CPU sec, 2.101 real sec... [M::process] read 690284 sequences (100000058 bp) in 1.340 CPU sec, 1.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 281622, 24, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 266, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (252.24, 130.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (308.47, 154.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 487, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2054) [M::mem_pestat] mean and std.dev: (552.59, 379.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 272, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (258.44, 151.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691020 reads in 161.629 CPU sec, 5.200 real sec [M::mem_process_seqs] Processed 690480 reads in 102.394 CPU sec, 3.327 real sec sorting block 95, #reads = 1069009 mem = 536882143 peak = 1227476992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281469, 38, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 285, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013) [M::mem_pestat] mean and std.dev: (466.62, 470.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (309.02, 154.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (724, 1013, 2183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5101) [M::mem_pestat] mean and std.dev: (1367.37, 1015.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6560) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 352, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2484) [M::mem_pestat] mean and std.dev: (528.08, 512.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3238) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690540 sequences (100000012 bp) in 2.077 CPU sec, 2.194 real sec... [M::process] read 690652 sequences (100000236 bp) in 1.350 CPU sec, 1.353 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 280933, 22, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 333, 2805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7957) [M::mem_pestat] mean and std.dev: (1328.16, 1722.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (308.20, 154.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 447, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2052) [M::mem_pestat] mean and std.dev: (518.80, 420.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 262, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (300.06, 176.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690372 reads in 159.018 CPU sec, 5.124 real sec sorting block 96, #reads = 1069259 mem = 536877119 peak = 1227476992 [M::process] read 690900 sequences (100000114 bp) in 1.523 CPU sec, 1.530 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 281252, 20, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (366.19, 280.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (307.62, 154.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (455, 1754, 6754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19352) [M::mem_pestat] mean and std.dev: (2516.30, 2576.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 299, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353) [M::mem_pestat] mean and std.dev: (357.07, 261.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690540 reads in 85.486 CPU sec, 2.759 real sec [M::mem_process_seqs] Processed 690284 reads in 213.318 CPU sec, 6.859 real sec sorting block 97, #reads = 1070319 mem = 536878255 peak = 1227476992 [M::process] read 691480 sequences (100000016 bp) in 1.973 CPU sec, 2.198 real sec... [M::process] read 690862 sequences (100000034 bp) in 1.350 CPU sec, 1.345 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 281330, 20, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 245, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137) [M::mem_pestat] mean and std.dev: (374.82, 322.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 282, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (306.06, 153.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (366, 617, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3732) [M::mem_pestat] mean and std.dev: (744.25, 525.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 341, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (343.22, 162.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690900 reads in 86.583 CPU sec, 2.778 real sec sorting block 98, #reads = 1070017 mem = 536880700 peak = 1227476992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281456, 11, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 495, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (390.04, 252.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (306.95, 153.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (488, 576, 1881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4667) [M::mem_pestat] mean and std.dev: (1016.60, 991.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 284, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1333) [M::mem_pestat] mean and std.dev: (353.42, 254.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 281842, 14, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 347, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2562) [M::mem_pestat] mean and std.dev: (421.84, 403.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 279, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (303.36, 152.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (792, 1102, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (106, 1821) [M::mem_pestat] mean and std.dev: (903.75, 374.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2401) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 351, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449) [M::mem_pestat] mean and std.dev: (351.77, 166.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690938 sequences (100000183 bp) in 1.559 CPU sec, 1.564 real sec... [M::mem_process_seqs] Processed 690652 reads in 265.226 CPU sec, 8.519 real sec [M::mem_process_seqs] Processed 691480 reads in 125.228 CPU sec, 4.054 real sec sorting block 99, #reads = 1069195 mem = 536881862 peak = 1227476992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 281548, 20, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 271, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (272.82, 148.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (305.92, 153.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 1095, 2436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6690) [M::mem_pestat] mean and std.dev: (1429.75, 1289.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 328, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (389.73, 264.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690462 sequences (100000199 bp) in 2.082 CPU sec, 2.223 real sec... [M::process] read 690386 sequences (100000091 bp) in 1.370 CPU sec, 1.366 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281574, 27, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 336, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2801) [M::mem_pestat] mean and std.dev: (537.93, 448.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (306.04, 153.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 617, 3083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9049) [M::mem_pestat] mean and std.dev: (1497.85, 1653.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12032) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 271, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (328.84, 197.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690862 reads in 156.584 CPU sec, 4.990 real sec [M::mem_process_seqs] Processed 690938 reads in 102.920 CPU sec, 3.546 real sec sorting block 100, #reads = 1069069 mem = 536882406 peak = 1227476992 [M::process] read 690492 sequences (100000034 bp) in 1.945 CPU sec, 2.076 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 281286, 18, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 398, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382) [M::mem_pestat] mean and std.dev: (431.35, 291.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 283, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (306.32, 152.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (392, 856, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2792) [M::mem_pestat] mean and std.dev: (949.83, 709.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 311, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (295.32, 110.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695004 sequences (100000287 bp) in 1.400 CPU sec, 1.397 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281387, 24, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 364, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1871) [M::mem_pestat] mean and std.dev: (379.92, 277.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 283, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (306.59, 152.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 334, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2455) [M::mem_pestat] mean and std.dev: (607.74, 546.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 277, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (324.48, 196.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690462 reads in 160.996 CPU sec, 5.392 real sec sorting block 101, #reads = 1069976 mem = 536879461 peak = 1227476992 [M::mem_process_seqs] Processed 690386 reads in 104.916 CPU sec, 3.361 real sec sorting block 102, #reads = 1068482 mem = 536879144 peak = 1229393920 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281519, 14, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 298, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (287.97, 178.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (306.87, 153.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 757, 1380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3736) [M::mem_pestat] mean and std.dev: (870.79, 601.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4914) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 289, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1847) [M::mem_pestat] mean and std.dev: (448.38, 373.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702320 sequences (100000086 bp) in 2.027 CPU sec, 2.147 real sec... [M::process] read 700912 sequences (100000289 bp) in 1.410 CPU sec, 1.416 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282883, 29, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 211, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (230.34, 102.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.25, 149.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 486, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4607) [M::mem_pestat] mean and std.dev: (799.61, 1099.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6119) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 263, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (256.30, 111.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690492 reads in 164.747 CPU sec, 5.252 real sec [M::mem_process_seqs] Processed 695004 reads in 112.077 CPU sec, 3.566 real sec sorting block 103, #reads = 1067107 mem = 536879960 peak = 1229393920 [M::process] read 699900 sequences (100000131 bp) in 2.011 CPU sec, 2.095 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285505, 12, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 254, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1642) [M::mem_pestat] mean and std.dev: (393.18, 384.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (268.47, 138.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 545, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3122) [M::mem_pestat] mean and std.dev: (509.09, 489.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 442, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2646) [M::mem_pestat] mean and std.dev: (543.20, 461.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699856 sequences (100000294 bp) in 1.430 CPU sec, 1.427 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285537, 16, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 220, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (260.00, 160.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (272.34, 139.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (470, 1807, 4503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12569) [M::mem_pestat] mean and std.dev: (2432.25, 2314.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 365, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3352) [M::mem_pestat] mean and std.dev: (629.89, 734.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702320 reads in 170.991 CPU sec, 5.450 real sec [M::mem_process_seqs] Processed 700912 reads in 111.149 CPU sec, 3.550 real sec sorting block 104, #reads = 1066317 mem = 536879970 peak = 1229393920 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285197, 19, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 231, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (250.89, 142.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 699226 sequences (100000036 bp) in 2.019 CPU sec, 2.139 real sec... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (277.04, 142.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (680, 1542, 5888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16304) [M::mem_pestat] mean and std.dev: (2710.00, 2584.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 238, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (214.84, 80.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697338 sequences (100000217 bp) in 1.410 CPU sec, 1.412 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 285021, 17, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 307, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (350.04, 223.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (275.20, 140.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (362, 1415, 2504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6788) [M::mem_pestat] mean and std.dev: (1457.47, 1306.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 388, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3449) [M::mem_pestat] mean and std.dev: (825.43, 965.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699900 reads in 171.805 CPU sec, 5.474 real sec sorting block 105, #reads = 1070131 mem = 536881561 peak = 1229393920 [M::mem_process_seqs] Processed 699856 reads in 113.484 CPU sec, 3.619 real sec sorting block 106, #reads = 1067075 mem = 536881409 peak = 1229963264 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285380, 20, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 249, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (237.72, 114.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 252, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (278.42, 141.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 486, 1696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4548) [M::mem_pestat] mean and std.dev: (1037.85, 1008.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 351, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757) [M::mem_pestat] mean and std.dev: (508.52, 444.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697196 sequences (100000295 bp) in 2.090 CPU sec, 2.195 real sec... [M::process] read 696440 sequences (100000097 bp) in 1.390 CPU sec, 1.393 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 283962, 21, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 377, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3947) [M::mem_pestat] mean and std.dev: (752.91, 740.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (283.03, 142.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 363, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3498) [M::mem_pestat] mean and std.dev: (516.40, 478.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4605) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (297.49, 142.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699226 reads in 174.757 CPU sec, 5.606 real sec [M::mem_process_seqs] Processed 697338 reads in 111.388 CPU sec, 3.627 real sec sorting block 107, #reads = 1067983 mem = 536878852 peak = 1229963264 [M::process] read 695312 sequences (100000084 bp) in 1.915 CPU sec, 2.002 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284035, 9, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 255, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (286.60, 232.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (284.70, 144.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 310, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1591) [M::mem_pestat] mean and std.dev: (414.97, 306.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694682 sequences (100000089 bp) in 1.400 CPU sec, 1.399 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284541, 32, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 501, 1947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5417) [M::mem_pestat] mean and std.dev: (997.26, 1171.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (287.78, 146.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 587, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3376) [M::mem_pestat] mean and std.dev: (735.33, 570.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 318, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108) [M::mem_pestat] mean and std.dev: (349.77, 176.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1409) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697196 reads in 171.116 CPU sec, 5.527 real sec [M::mem_process_seqs] Processed 696440 reads in 107.432 CPU sec, 3.428 real sec sorting block 108, #reads = 1067050 mem = 536877900 peak = 1229963264 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 283530, 22, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 388, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (309.60, 146.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (291.78, 147.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (535, 914, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3694) [M::mem_pestat] mean and std.dev: (992.95, 662.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 258, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (260.86, 137.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693838 sequences (100000089 bp) in 2.017 CPU sec, 2.137 real sec... [M::process] read 693792 sequences (100000032 bp) in 1.370 CPU sec, 1.368 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 283955, 6, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 268, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443) [M::mem_pestat] mean and std.dev: (351.23, 305.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (292.81, 147.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 372, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2436) [M::mem_pestat] mean and std.dev: (601.98, 575.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3180) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695312 reads in 159.967 CPU sec, 5.109 real sec sorting block 109, #reads = 1070097 mem = 536880889 peak = 1229963264 [M::mem_process_seqs] Processed 694682 reads in 104.486 CPU sec, 3.345 real sec sorting block 110, #reads = 1070785 mem = 536884303 peak = 1236385792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283243, 12, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 211, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1710) [M::mem_pestat] mean and std.dev: (350.40, 304.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (295.93, 148.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (465, 669, 1789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4437) [M::mem_pestat] mean and std.dev: (829.73, 542.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 331, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1982) [M::mem_pestat] mean and std.dev: (507.26, 452.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693330 sequences (100000054 bp) in 1.978 CPU sec, 2.076 real sec... [M::process] read 693528 sequences (100000158 bp) in 1.370 CPU sec, 1.369 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 283281, 20, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 346, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (365.78, 215.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 272, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (296.44, 149.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 470, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1737) [M::mem_pestat] mean and std.dev: (511.68, 301.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2168) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 349, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (347.89, 237.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693838 reads in 158.675 CPU sec, 5.061 real sec [M::mem_process_seqs] Processed 693792 reads in 104.572 CPU sec, 3.593 real sec sorting block 111, #reads = 1070470 mem = 536880552 peak = 1236385792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282879, 18, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 276, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (293.67, 179.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 274, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (298.34, 150.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 511, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370) [M::mem_pestat] mean and std.dev: (426.94, 345.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1808) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 385, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (351.09, 174.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693148 sequences (100000108 bp) in 2.092 CPU sec, 2.224 real sec... [M::process] read 693128 sequences (100000001 bp) in 1.360 CPU sec, 1.367 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 283272, 23, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 359, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1518) [M::mem_pestat] mean and std.dev: (354.50, 209.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (298.46, 152.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1366, 2202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5390) [M::mem_pestat] mean and std.dev: (1481.70, 1272.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6984) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 430, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2448) [M::mem_pestat] mean and std.dev: (599.77, 531.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693330 reads in 162.292 CPU sec, 5.426 real sec [M::mem_process_seqs] Processed 693528 reads in 105.597 CPU sec, 3.377 real sec sorting block 112, #reads = 1070317 mem = 536881256 peak = 1236385792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282789, 19, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 263, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2724) [M::mem_pestat] mean and std.dev: (368.18, 344.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 275, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (300.84, 153.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 406, 3169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9063) [M::mem_pestat] mean and std.dev: (1372.37, 1498.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 370, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2825) [M::mem_pestat] mean and std.dev: (531.98, 499.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692042 sequences (100000001 bp) in 1.991 CPU sec, 2.110 real sec... [M::process] read 691952 sequences (100000125 bp) in 1.380 CPU sec, 1.377 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282662, 18, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 357, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (331.21, 175.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (297.97, 150.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (731, 1775, 4253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11297) [M::mem_pestat] mean and std.dev: (2314.72, 2295.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 392, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3398) [M::mem_pestat] mean and std.dev: (692.24, 861.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693148 reads in 158.844 CPU sec, 5.063 real sec [M::mem_process_seqs] Processed 693128 reads in 104.144 CPU sec, 3.317 real sec sorting block 113, #reads = 1071327 mem = 536879153 peak = 1236385792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 281742, 19, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 297, 1434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3894) [M::mem_pestat] mean and std.dev: (775.56, 930.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 280, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (304.13, 153.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (409, 953, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2056) [M::mem_pestat] mean and std.dev: (596.94, 400.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2605) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (291, 616, 2648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7362) [M::mem_pestat] mean and std.dev: (1523.57, 1698.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9719) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691282 sequences (100000152 bp) in 2.121 CPU sec, 2.224 real sec... [M::process] read 691160 sequences (100000071 bp) in 1.360 CPU sec, 1.362 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 282113, 17, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 423, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861) [M::mem_pestat] mean and std.dev: (336.60, 192.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (304.95, 154.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 666, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1988) [M::mem_pestat] mean and std.dev: (549.27, 278.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 324, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (367.16, 216.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692042 reads in 163.005 CPU sec, 5.253 real sec sorting block 114, #reads = 1070714 mem = 536884372 peak = 1236385792 [M::mem_process_seqs] Processed 691952 reads in 104.173 CPU sec, 3.344 real sec sorting block 115, #reads = 1069845 mem = 536882899 peak = 1237508096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281697, 25, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 407, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3299) [M::mem_pestat] mean and std.dev: (641.41, 634.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (306.56, 155.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 811, 1965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4693) [M::mem_pestat] mean and std.dev: (1043.75, 803.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 316, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (401.34, 299.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690934 sequences (100000038 bp) in 1.878 CPU sec, 1.978 real sec... [M::process] read 690836 sequences (100000179 bp) in 1.370 CPU sec, 1.364 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 281831, 16, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 268, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (310.08, 189.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (307.45, 155.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (519, 1155, 1856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4530) [M::mem_pestat] mean and std.dev: (965.21, 525.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 281, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433) [M::mem_pestat] mean and std.dev: (311.36, 272.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691282 reads in 161.235 CPU sec, 5.176 real sec [M::mem_process_seqs] Processed 691160 reads in 103.251 CPU sec, 3.398 real sec sorting block 116, #reads = 1069922 mem = 536878600 peak = 1237508096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 281347, 14, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 336, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (371.54, 235.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (308.00, 156.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 1278, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4934) [M::mem_pestat] mean and std.dev: (1144.64, 1081.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 315, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (293.34, 169.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691042 sequences (100000123 bp) in 2.048 CPU sec, 2.173 real sec... [M::process] read 691026 sequences (100000259 bp) in 1.360 CPU sec, 1.361 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 281756, 30, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 272, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (303.47, 204.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (307.57, 156.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 632, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744) [M::mem_pestat] mean and std.dev: (689.40, 609.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 390, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (349.09, 137.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690934 reads in 159.740 CPU sec, 5.188 real sec [M::mem_process_seqs] Processed 690836 reads in 104.203 CPU sec, 3.326 real sec sorting block 117, #reads = 1070898 mem = 536879754 peak = 1237508096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 281176, 25, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 379, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2786) [M::mem_pestat] mean and std.dev: (547.28, 576.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (306.99, 156.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 956, 2056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5764) [M::mem_pestat] mean and std.dev: (847.77, 704.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7618) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 445, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1921) [M::mem_pestat] mean and std.dev: (518.91, 393.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691464 sequences (100000176 bp) in 2.004 CPU sec, 2.106 real sec... [M::process] read 691170 sequences (100000220 bp) in 1.390 CPU sec, 1.389 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 282047, 22, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 341, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2597) [M::mem_pestat] mean and std.dev: (575.82, 652.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (309.47, 158.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 329, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142) [M::mem_pestat] mean and std.dev: (362.14, 315.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 490, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3495) [M::mem_pestat] mean and std.dev: (801.35, 741.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691042 reads in 155.746 CPU sec, 4.976 real sec sorting block 118, #reads = 1069961 mem = 536877964 peak = 1237508096 [M::mem_process_seqs] Processed 691026 reads in 102.765 CPU sec, 3.555 real sec sorting block 119, #reads = 1070473 mem = 536883536 peak = 1237508096 [M::process] read 690672 sequences (100000169 bp) in 2.001 CPU sec, 2.080 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 281513, 23, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 420, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166) [M::mem_pestat] mean and std.dev: (443.18, 372.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (308.01, 157.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (499, 1379, 2891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7675) [M::mem_pestat] mean and std.dev: (1595.17, 1301.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 375, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (510.42, 427.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691288 sequences (100000142 bp) in 1.408 CPU sec, 1.410 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 281423, 13, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 311, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454) [M::mem_pestat] mean and std.dev: (373.11, 229.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 284, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (311.71, 159.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (247, 523, 3453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9865) [M::mem_pestat] mean and std.dev: (1351.23, 1690.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13071) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 292, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (265.06, 129.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691464 reads in 163.171 CPU sec, 5.472 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 281347, 20, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 288, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3046) [M::mem_pestat] mean and std.dev: (641.48, 731.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (309.16, 158.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 651, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (584.89, 365.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 340, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (513.65, 476.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698598 sequences (100000134 bp) in 1.523 CPU sec, 1.532 real sec... [M::mem_process_seqs] Processed 690672 reads in 174.009 CPU sec, 5.531 real sec sorting block 120, #reads = 1068744 mem = 536878262 peak = 1237508096 [M::mem_process_seqs] Processed 691170 reads in 192.142 CPU sec, 6.406 real sec sorting block 121, #reads = 1068706 mem = 536879202 peak = 1237508096 [M::process] read 699194 sequences (100000039 bp) in 2.022 CPU sec, 2.111 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281314, 30, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 408, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (367.93, 184.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (309.41, 158.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (713, 1440, 1709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3701) [M::mem_pestat] mean and std.dev: (1315.48, 871.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 385, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2253) [M::mem_pestat] mean and std.dev: (564.50, 522.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699500 sequences (100000005 bp) in 1.368 CPU sec, 1.372 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285056, 12, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 285, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2196) [M::mem_pestat] mean and std.dev: (364.33, 315.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 251, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (279.34, 144.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (514, 947, 2268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5776) [M::mem_pestat] mean and std.dev: (1007.00, 728.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 297, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690) [M::mem_pestat] mean and std.dev: (313.97, 202.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284215, 20, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 404, 1287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3431) [M::mem_pestat] mean and std.dev: (638.13, 613.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (281.85, 146.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 589, 2964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8534) [M::mem_pestat] mean and std.dev: (1408.11, 1925.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 271, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134) [M::mem_pestat] mean and std.dev: (339.56, 214.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691288 reads in 241.630 CPU sec, 8.004 real sec [M::mem_process_seqs] Processed 699194 reads in 178.684 CPU sec, 5.738 real sec [M::mem_process_seqs] Processed 698598 reads in 199.242 CPU sec, 6.422 real sec sorting block 122, #reads = 1065074 mem = 536882134 peak = 1237508096 [M::process] read 698366 sequences (100000184 bp) in 2.354 CPU sec, 2.488 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284902, 23, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 480, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (399.41, 285.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (279.60, 145.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 830, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2255) [M::mem_pestat] mean and std.dev: (703.25, 451.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2888) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 438, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1308) [M::mem_pestat] mean and std.dev: (413.77, 228.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 123, #reads = 1067983 mem = 536880251 peak = 1237508096 [M::process] read 696916 sequences (100000120 bp) in 1.739 CPU sec, 1.833 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284848, 29, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 340, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (352.88, 203.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (282.05, 145.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 989, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3514) [M::mem_pestat] mean and std.dev: (948.83, 701.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4559) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 320, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136) [M::mem_pestat] mean and std.dev: (364.57, 187.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699500 reads in 160.955 CPU sec, 5.140 real sec [M::process] read 696580 sequences (100000023 bp) in 1.402 CPU sec, 1.423 real sec... [M::mem_process_seqs] Processed 698366 reads in 105.577 CPU sec, 3.342 real sec sorting block 124, #reads = 1074585 mem = 536882507 peak = 1237508096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284205, 18, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 242, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (286.80, 184.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (286.60, 147.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 1268, 1399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917) [M::mem_pestat] mean and std.dev: (1079.82, 610.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (321, 480, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2346) [M::mem_pestat] mean and std.dev: (594.02, 430.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3021) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696022 sequences (100000127 bp) in 2.084 CPU sec, 2.215 real sec... [M::process] read 694670 sequences (100000077 bp) in 1.340 CPU sec, 1.343 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284403, 12, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 326, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (323.18, 189.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (289.70, 150.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (496, 947, 2654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6970) [M::mem_pestat] mean and std.dev: (1318.83, 1130.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 280, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504) [M::mem_pestat] mean and std.dev: (315.44, 234.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696916 reads in 155.732 CPU sec, 5.184 real sec [M::mem_process_seqs] Processed 696580 reads in 99.129 CPU sec, 3.165 real sec sorting block 125, #reads = 1074959 mem = 536877582 peak = 1237508096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283898, 14, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 220, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (251.34, 164.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (290.04, 149.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (329, 745, 1944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5174) [M::mem_pestat] mean and std.dev: (1493.29, 1655.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8114) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 275, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (352.27, 247.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1433) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693878 sequences (100000018 bp) in 1.948 CPU sec, 2.079 real sec... [M::process] read 694262 sequences (100000096 bp) in 1.350 CPU sec, 1.354 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283629, 10, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 460, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2506) [M::mem_pestat] mean and std.dev: (554.17, 497.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (293.64, 149.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (327, 983, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3489) [M::mem_pestat] mean and std.dev: (743.00, 518.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 341, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (322.45, 126.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696022 reads in 149.651 CPU sec, 4.796 real sec [M::mem_process_seqs] Processed 694670 reads in 97.193 CPU sec, 3.146 real sec sorting block 126, #reads = 1076557 mem = 536879981 peak = 1237508096 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282931, 16, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 305, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (280.72, 133.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (296.72, 150.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 475, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2788) [M::mem_pestat] mean and std.dev: (324.31, 318.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 283, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1484) [M::mem_pestat] mean and std.dev: (365.90, 277.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693418 sequences (100000066 bp) in 2.060 CPU sec, 2.193 real sec... [M::process] read 693744 sequences (100000123 bp) in 1.340 CPU sec, 1.339 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 283111, 14, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 343, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859) [M::mem_pestat] mean and std.dev: (398.10, 350.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (293.39, 149.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 818, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4609) [M::mem_pestat] mean and std.dev: (1105.93, 867.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6064) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 348, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2900) [M::mem_pestat] mean and std.dev: (442.80, 402.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693878 reads in 152.658 CPU sec, 4.897 real sec sorting block 127, #reads = 1076207 mem = 536877477 peak = 1237508096 [M::mem_process_seqs] Processed 694262 reads in 95.938 CPU sec, 3.054 real sec sorting block 128, #reads = 1076296 mem = 536880705 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 283123, 15, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 259, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (241.02, 90.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (297.71, 151.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (455, 937, 1959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4967) [M::mem_pestat] mean and std.dev: (1034.00, 767.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 294, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (296.51, 131.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693516 sequences (100000296 bp) in 1.906 CPU sec, 2.085 real sec... [M::process] read 693642 sequences (100000112 bp) in 1.350 CPU sec, 1.351 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283218, 24, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 315, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3158) [M::mem_pestat] mean and std.dev: (692.09, 791.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (296.67, 150.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (385, 722, 1771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4543) [M::mem_pestat] mean and std.dev: (890.82, 930.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 334, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183) [M::mem_pestat] mean and std.dev: (395.10, 265.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1508) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693418 reads in 150.953 CPU sec, 4.867 real sec [M::mem_process_seqs] Processed 693744 reads in 95.639 CPU sec, 3.054 real sec sorting block 129, #reads = 1075324 mem = 536877592 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 282862, 17, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 311, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405) [M::mem_pestat] mean and std.dev: (327.47, 228.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (296.90, 149.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 295, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3950) [M::mem_pestat] mean and std.dev: (691.00, 675.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 576, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2835) [M::mem_pestat] mean and std.dev: (545.60, 436.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3714) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692802 sequences (100000049 bp) in 2.110 CPU sec, 2.224 real sec... [M::process] read 693084 sequences (100000159 bp) in 1.340 CPU sec, 1.344 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283069, 17, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 409, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538) [M::mem_pestat] mean and std.dev: (419.85, 258.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (296.03, 148.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (215, 619, 5034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14672) [M::mem_pestat] mean and std.dev: (1742.12, 2082.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19491) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 261, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (307.03, 156.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693516 reads in 150.594 CPU sec, 4.816 real sec sorting block 130, #reads = 1074037 mem = 536879837 peak = 1258979328 [M::mem_process_seqs] Processed 693642 reads in 100.666 CPU sec, 3.225 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282402, 13, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 435, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3676) [M::mem_pestat] mean and std.dev: (629.88, 630.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (298.85, 150.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 933, 2494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6858) [M::mem_pestat] mean and std.dev: (1439.50, 1376.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 305, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1759) [M::mem_pestat] mean and std.dev: (373.35, 292.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693512 sequences (100000219 bp) in 2.019 CPU sec, 2.126 real sec... [M::process] read 694378 sequences (100000176 bp) in 1.360 CPU sec, 1.355 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283134, 17, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 243, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3010) [M::mem_pestat] mean and std.dev: (429.05, 417.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (298.62, 150.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1450, 2939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7475) [M::mem_pestat] mean and std.dev: (1543.88, 1026.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 384, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1542) [M::mem_pestat] mean and std.dev: (394.89, 264.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692802 reads in 151.221 CPU sec, 4.812 real sec sorting block 131, #reads = 1073364 mem = 536878797 peak = 1258979328 [M::mem_process_seqs] Processed 693084 reads in 99.892 CPU sec, 3.188 real sec sorting block 132, #reads = 1072692 mem = 536880299 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 282892, 8, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 294, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (263.91, 105.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (297.26, 151.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 303, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (304.84, 205.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693850 sequences (100000157 bp) in 2.098 CPU sec, 2.201 real sec... [M::process] read 694050 sequences (100000042 bp) in 1.350 CPU sec, 1.355 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283419, 18, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 307, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1495) [M::mem_pestat] mean and std.dev: (382.48, 256.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (292.57, 147.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (576, 1227, 2096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5136) [M::mem_pestat] mean and std.dev: (1517.06, 1382.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 384, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (348.70, 185.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693512 reads in 157.448 CPU sec, 5.031 real sec [M::mem_process_seqs] Processed 694378 reads in 99.567 CPU sec, 3.197 real sec sorting block 133, #reads = 1074901 mem = 536885262 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282759, 26, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 184, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192) [M::mem_pestat] mean and std.dev: (350.93, 441.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (294.20, 149.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (161, 1250, 1469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4085) [M::mem_pestat] mean and std.dev: (816.96, 656.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5393) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 354, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1393) [M::mem_pestat] mean and std.dev: (379.79, 269.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694128 sequences (100000235 bp) in 1.991 CPU sec, 2.107 real sec... [M::process] read 692432 sequences (100000121 bp) in 1.350 CPU sec, 1.355 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282362, 14, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 323, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1508) [M::mem_pestat] mean and std.dev: (359.66, 278.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (292.46, 148.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (493, 882, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2446) [M::mem_pestat] mean and std.dev: (697.85, 373.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 334, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (344.97, 220.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1504) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693850 reads in 157.313 CPU sec, 5.051 real sec sorting block 134, #reads = 1074375 mem = 536878454 peak = 1258979328 [M::process] read 692756 sequences (100000007 bp) in 1.592 CPU sec, 1.595 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283541, 25, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 298, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (257.26, 93.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (295.93, 151.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (88, 606, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4300) [M::mem_pestat] mean and std.dev: (791.91, 944.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 286, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1929) [M::mem_pestat] mean and std.dev: (503.68, 447.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694128 reads in 84.857 CPU sec, 2.803 real sec [M::mem_process_seqs] Processed 694050 reads in 208.998 CPU sec, 6.778 real sec sorting block 135, #reads = 1074022 mem = 536881966 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 282298, 22, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 276, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (271.51, 138.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 276, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (303.29, 155.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (334, 710, 1724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4504) [M::mem_pestat] mean and std.dev: (1012.41, 852.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 316, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (384.93, 263.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691938 sequences (100000238 bp) in 1.907 CPU sec, 2.011 real sec... [M::process] read 691802 sequences (100000301 bp) in 1.340 CPU sec, 1.344 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282368, 17, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 270, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (318.93, 213.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 277, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (303.56, 155.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 624, 1991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5713) [M::mem_pestat] mean and std.dev: (968.31, 965.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 355, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (358.05, 218.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692432 reads in 173.963 CPU sec, 5.588 real sec [M::mem_process_seqs] Processed 692756 reads in 100.556 CPU sec, 3.261 real sec sorting block 136, #reads = 1071754 mem = 536877591 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282120, 10, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 232, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (259.09, 159.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 278, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (304.22, 154.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (376, 860, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2983) [M::mem_pestat] mean and std.dev: (540.50, 396.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 408, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2847) [M::mem_pestat] mean and std.dev: (596.11, 601.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691734 sequences (100000000 bp) in 2.117 CPU sec, 2.292 real sec... [M::process] read 691280 sequences (100000143 bp) in 1.360 CPU sec, 1.360 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 282078, 19, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 250, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (305.65, 205.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 279, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (305.10, 155.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (360, 427, 1486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3738) [M::mem_pestat] mean and std.dev: (856.74, 848.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 276, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2512) [M::mem_pestat] mean and std.dev: (557.02, 532.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691938 reads in 158.874 CPU sec, 5.125 real sec sorting block 137, #reads = 1073235 mem = 536879123 peak = 1258979328 [M::mem_process_seqs] Processed 691802 reads in 101.682 CPU sec, 3.337 real sec sorting block 138, #reads = 1070367 mem = 536879711 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 281941, 28, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 264, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279) [M::mem_pestat] mean and std.dev: (306.40, 213.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (305.37, 155.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (134, 579, 2975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8657) [M::mem_pestat] mean and std.dev: (1247.14, 1578.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 336, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1733) [M::mem_pestat] mean and std.dev: (356.98, 247.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692568 sequences (100000201 bp) in 2.057 CPU sec, 2.173 real sec... [M::process] read 692172 sequences (100000185 bp) in 1.370 CPU sec, 1.376 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 281676, 17, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 359, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1556) [M::mem_pestat] mean and std.dev: (381.84, 288.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (308.69, 157.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 500, 3324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9344) [M::mem_pestat] mean and std.dev: (1325.35, 1481.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 297, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (310.19, 145.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691734 reads in 158.865 CPU sec, 5.149 real sec [M::mem_process_seqs] Processed 691280 reads in 101.482 CPU sec, 3.234 real sec sorting block 139, #reads = 1070278 mem = 536877343 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282200, 22, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 238, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3154) [M::mem_pestat] mean and std.dev: (626.71, 675.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 275, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (302.68, 155.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (380, 878, 2240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5960) [M::mem_pestat] mean and std.dev: (1342.95, 1439.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 292, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (248.24, 109.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692074 sequences (100000083 bp) in 2.014 CPU sec, 2.128 real sec... [M::process] read 701072 sequences (100000255 bp) in 1.420 CPU sec, 1.415 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 281999, 21, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 309, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1720) [M::mem_pestat] mean and std.dev: (468.02, 402.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 276, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (303.43, 155.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 763, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3040) [M::mem_pestat] mean and std.dev: (867.25, 687.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 363, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267) [M::mem_pestat] mean and std.dev: (482.77, 391.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692568 reads in 165.511 CPU sec, 5.330 real sec [M::mem_process_seqs] Processed 692172 reads in 106.674 CPU sec, 3.475 real sec sorting block 140, #reads = 1070445 mem = 536879556 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 281639, 15, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 363, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2040) [M::mem_pestat] mean and std.dev: (558.21, 533.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 278, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (305.87, 157.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (774, 1159, 2007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4473) [M::mem_pestat] mean and std.dev: (1129.62, 626.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 346, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (370.56, 198.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1431) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702646 sequences (100000091 bp) in 2.110 CPU sec, 2.205 real sec... [M::process] read 701974 sequences (100000010 bp) in 1.420 CPU sec, 1.416 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284098, 24, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 295, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (253.22, 107.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (272.56, 141.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (393, 830, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2835) [M::mem_pestat] mean and std.dev: (826.75, 561.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 277, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (317.98, 169.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692074 reads in 183.068 CPU sec, 5.906 real sec sorting block 141, #reads = 1065494 mem = 536882710 peak = 1258979328 [M::mem_process_seqs] Processed 701072 reads in 120.245 CPU sec, 3.841 real sec [M::process] read 702924 sequences (100000262 bp) in 2.044 CPU sec, 2.144 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285793, 19, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 348, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1809) [M::mem_pestat] mean and std.dev: (442.36, 364.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (268.77, 138.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (617, 786, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859) [M::mem_pestat] mean and std.dev: (793.47, 378.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2306) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 317, 1190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3180) [M::mem_pestat] mean and std.dev: (645.78, 597.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4175) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700712 sequences (100000273 bp) in 1.400 CPU sec, 1.405 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285946, 13, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 293, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682) [M::mem_pestat] mean and std.dev: (281.32, 174.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (270.18, 139.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (97, 333, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1972) [M::mem_pestat] mean and std.dev: (367.25, 323.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 246, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (241.32, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702646 reads in 167.659 CPU sec, 5.338 real sec sorting block 142, #reads = 1061864 mem = 536880171 peak = 1258979328 [M::mem_process_seqs] Processed 701974 reads in 110.298 CPU sec, 3.527 real sec sorting block 143, #reads = 1071843 mem = 536879588 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285831, 12, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 190, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (223.79, 170.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (266.95, 137.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 1006, 1856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4924) [M::mem_pestat] mean and std.dev: (1069.42, 1000.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 255, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (273.80, 118.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700848 sequences (100000230 bp) in 2.177 CPU sec, 2.276 real sec... [M::process] read 700804 sequences (100000063 bp) in 1.410 CPU sec, 1.419 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285467, 23, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 408, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2286) [M::mem_pestat] mean and std.dev: (491.95, 373.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (273.95, 139.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (542, 1002, 2228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5600) [M::mem_pestat] mean and std.dev: (1077.55, 855.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 323, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (339.27, 159.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702924 reads in 173.417 CPU sec, 5.528 real sec [M::mem_process_seqs] Processed 700712 reads in 111.563 CPU sec, 3.561 real sec sorting block 144, #reads = 1068805 mem = 536886040 peak = 1258979328 [M::process] read 698558 sequences (100000100 bp) in 1.934 CPU sec, 2.017 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285419, 9, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 213, 994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2652) [M::mem_pestat] mean and std.dev: (615.88, 749.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (271.87, 139.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 330, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358) [M::mem_pestat] mean and std.dev: (372.74, 221.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1737) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700848 reads in 96.832 CPU sec, 3.210 real sec sorting block 145, #reads = 1069906 mem = 536885459 peak = 1258979328 [M::process] read 697268 sequences (100000019 bp) in 1.887 CPU sec, 2.000 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285659, 27, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 278, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (263.94, 160.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (273.51, 139.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (362, 577, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1694) [M::mem_pestat] mean and std.dev: (589.04, 361.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 339, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469) [M::mem_pestat] mean and std.dev: (457.31, 387.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696304 sequences (100000079 bp) in 1.460 CPU sec, 1.464 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 284750, 24, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 383, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348) [M::mem_pestat] mean and std.dev: (409.34, 261.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (278.16, 140.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (138, 807, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3447) [M::mem_pestat] mean and std.dev: (699.00, 486.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 280, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (284.32, 137.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700804 reads in 192.916 CPU sec, 6.187 real sec [M::mem_process_seqs] Processed 698558 reads in 206.964 CPU sec, 6.742 real sec sorting block 146, #reads = 1068806 mem = 536881647 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284360, 21, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 267, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (302.84, 176.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (284.36, 144.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (723, 1386, 4253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11313) [M::mem_pestat] mean and std.dev: (2700.62, 2590.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14843) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 218, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (274.58, 167.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696014 sequences (100000272 bp) in 2.165 CPU sec, 2.313 real sec... [M::process] read 695420 sequences (100000179 bp) in 1.350 CPU sec, 1.350 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 283768, 22, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 262, 854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2268) [M::mem_pestat] mean and std.dev: (497.14, 565.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (287.79, 146.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 1354, 1549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3937) [M::mem_pestat] mean and std.dev: (1154.50, 750.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 262, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (364.14, 262.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697268 reads in 166.558 CPU sec, 5.407 real sec [M::mem_process_seqs] Processed 696304 reads in 105.072 CPU sec, 3.365 real sec sorting block 147, #reads = 1070793 mem = 536879635 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283656, 14, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 210, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (274.94, 190.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (289.53, 147.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 351, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2412) [M::mem_pestat] mean and std.dev: (422.85, 358.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 358, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (379.67, 246.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695660 sequences (100000276 bp) in 2.012 CPU sec, 2.149 real sec... [M::process] read 695120 sequences (100000009 bp) in 1.380 CPU sec, 1.379 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 283594, 12, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 340, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974) [M::mem_pestat] mean and std.dev: (471.67, 335.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (291.21, 148.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 491, 2170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5888) [M::mem_pestat] mean and std.dev: (896.17, 863.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 365, 1924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5314) [M::mem_pestat] mean and std.dev: (1036.24, 1176.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696014 reads in 163.602 CPU sec, 5.241 real sec sorting block 148, #reads = 1071285 mem = 536888738 peak = 1258979328 [M::mem_process_seqs] Processed 695420 reads in 102.460 CPU sec, 3.295 real sec sorting block 149, #reads = 1072097 mem = 536878796 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284206, 33, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 291, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789) [M::mem_pestat] mean and std.dev: (436.40, 337.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 267, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (292.66, 149.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (150, 933, 1419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3957) [M::mem_pestat] mean and std.dev: (1016.85, 1032.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 229, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (236.19, 91.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694738 sequences (100000080 bp) in 2.086 CPU sec, 2.203 real sec... [M::process] read 694840 sequences (100000289 bp) in 1.340 CPU sec, 1.346 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283355, 12, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 265, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1765) [M::mem_pestat] mean and std.dev: (413.18, 388.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (293.08, 149.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (983, 1291, 2234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4736) [M::mem_pestat] mean and std.dev: (1489.42, 994.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 401, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454) [M::mem_pestat] mean and std.dev: (460.00, 367.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695660 reads in 161.879 CPU sec, 5.202 real sec [M::mem_process_seqs] Processed 695120 reads in 103.641 CPU sec, 3.416 real sec sorting block 150, #reads = 1071628 mem = 536879526 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282981, 10, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 362, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1885) [M::mem_pestat] mean and std.dev: (518.94, 434.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (294.69, 150.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 455, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3703) [M::mem_pestat] mean and std.dev: (607.00, 802.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4898) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 484, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3679) [M::mem_pestat] mean and std.dev: (767.32, 871.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694164 sequences (100000076 bp) in 2.027 CPU sec, 2.154 real sec... [M::process] read 693426 sequences (100000089 bp) in 1.350 CPU sec, 1.354 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283217, 11, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 303, 2239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6399) [M::mem_pestat] mean and std.dev: (1194.61, 1846.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (296.21, 151.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 291, 1052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2616) [M::mem_pestat] mean and std.dev: (468.80, 395.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 378, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742) [M::mem_pestat] mean and std.dev: (420.62, 266.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694738 reads in 162.837 CPU sec, 5.290 real sec [M::mem_process_seqs] Processed 694840 reads in 102.921 CPU sec, 3.358 real sec sorting block 151, #reads = 1072232 mem = 536878489 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282626, 17, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 297, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (299.23, 156.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (297.72, 152.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (590, 1300, 3099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8117) [M::mem_pestat] mean and std.dev: (1977.88, 1389.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 249, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1408) [M::mem_pestat] mean and std.dev: (294.21, 234.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693030 sequences (100000260 bp) in 2.107 CPU sec, 2.223 real sec... [M::process] read 692968 sequences (100000141 bp) in 1.340 CPU sec, 1.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 283069, 22, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 331, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3060) [M::mem_pestat] mean and std.dev: (623.24, 584.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 275, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (299.82, 152.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 634, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3362) [M::mem_pestat] mean and std.dev: (691.14, 571.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4416) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 632, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2283) [M::mem_pestat] mean and std.dev: (562.23, 349.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694164 reads in 161.582 CPU sec, 5.238 real sec sorting block 152, #reads = 1072009 mem = 536882571 peak = 1258979328 [M::mem_process_seqs] Processed 693426 reads in 102.042 CPU sec, 3.320 real sec sorting block 153, #reads = 1071753 mem = 536880036 peak = 1258979328 [M::process] read 692270 sequences (100000019 bp) in 1.956 CPU sec, 2.048 real sec... [M::process] read 692004 sequences (100000242 bp) in 1.330 CPU sec, 1.338 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 282888, 32, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 293, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (279.63, 104.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (298.55, 149.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (280, 740, 2270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6250) [M::mem_pestat] mean and std.dev: (864.61, 789.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 313, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (526.98, 442.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692968 reads in 83.578 CPU sec, 2.696 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282733, 28, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 291, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2701) [M::mem_pestat] mean and std.dev: (427.51, 419.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 277, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (300.64, 151.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (385, 1038, 1534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3832) [M::mem_pestat] mean and std.dev: (1067.64, 817.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4981) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 290, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334) [M::mem_pestat] mean and std.dev: (295.03, 208.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691162 sequences (100000091 bp) in 1.391 CPU sec, 1.392 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 282412, 20, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 561, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2456) [M::mem_pestat] mean and std.dev: (635.94, 576.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 279, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (303.13, 153.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 861, 2340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6248) [M::mem_pestat] mean and std.dev: (1601.45, 1986.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 317, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1931) [M::mem_pestat] mean and std.dev: (437.09, 329.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693030 reads in 241.635 CPU sec, 7.808 real sec sorting block 154, #reads = 1072767 mem = 536882505 peak = 1258979328 [M::mem_process_seqs] Processed 692270 reads in 116.345 CPU sec, 3.790 real sec sorting block 155, #reads = 1072827 mem = 536877899 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282391, 20, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 477, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2639) [M::mem_pestat] mean and std.dev: (632.74, 566.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 282, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (305.91, 154.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (754, 2766, 3458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8866) [M::mem_pestat] mean and std.dev: (1987.89, 1749.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 358, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (361.11, 194.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691330 sequences (100000054 bp) in 2.059 CPU sec, 2.182 real sec... [M::process] read 691188 sequences (100000204 bp) in 1.320 CPU sec, 1.321 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 281487, 17, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 379, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (332.02, 150.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 284, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (307.78, 154.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 697, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853) [M::mem_pestat] mean and std.dev: (470.38, 295.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 285, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344) [M::mem_pestat] mean and std.dev: (342.22, 230.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692004 reads in 156.286 CPU sec, 5.014 real sec [M::mem_process_seqs] Processed 691162 reads in 100.498 CPU sec, 3.283 real sec sorting block 156, #reads = 1071209 mem = 536882180 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282402, 15, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 276, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (288.68, 120.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (308.09, 154.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (427, 556, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2020) [M::mem_pestat] mean and std.dev: (551.92, 240.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 490, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1557) [M::mem_pestat] mean and std.dev: (533.24, 381.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691326 sequences (100000169 bp) in 2.054 CPU sec, 2.223 real sec... [M::process] read 690056 sequences (100000160 bp) in 1.330 CPU sec, 1.328 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281833, 17, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 262, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (332.07, 268.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 284, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (306.90, 153.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (683, 2097, 4443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11963) [M::mem_pestat] mean and std.dev: (2406.29, 1608.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 287, 841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2095) [M::mem_pestat] mean and std.dev: (483.14, 467.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691330 reads in 155.182 CPU sec, 5.014 real sec [M::mem_process_seqs] Processed 691188 reads in 98.391 CPU sec, 3.202 real sec sorting block 157, #reads = 1071459 mem = 536881935 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281437, 20, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 274, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (319.81, 233.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (305.03, 152.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (103, 680, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3508) [M::mem_pestat] mean and std.dev: (716.32, 670.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 287, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1447) [M::mem_pestat] mean and std.dev: (345.03, 288.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690362 sequences (100000105 bp) in 1.920 CPU sec, 2.027 real sec... [M::process] read 343224 sequences (49560584 bp) in 0.820 CPU sec, 0.826 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281113, 12, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 270, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (334.17, 231.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (308.18, 153.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (399, 515, 4177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11733) [M::mem_pestat] mean and std.dev: (1510.33, 1715.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15511) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 390, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (497.60, 394.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691326 reads in 154.100 CPU sec, 5.006 real sec [M::mem_process_seqs] Processed 690056 reads in 97.192 CPU sec, 3.148 real sec sorting block 158, #reads = 1071763 mem = 536879572 peak = 1258979328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 281069, 21, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 285, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1707) [M::mem_pestat] mean and std.dev: (451.58, 388.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 285, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (307.09, 152.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 908, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3703) [M::mem_pestat] mean and std.dev: (903.35, 616.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 371, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (352.18, 193.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 139832, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 437, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3132) [M::mem_pestat] mean and std.dev: (424.95, 268.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 276, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (300.10, 150.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 359, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (356.84, 158.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690362 reads in 118.171 CPU sec, 3.868 real sec sorting block 159, #reads = 1070191 mem = 536882091 peak = 1291272192 [M::mem_process_seqs] Processed 343224 reads in 61.040 CPU sec, 1.974 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 171461998 overall: 70146220032 mem 21882.220 user 441.945 sys 1751.565 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_FRC-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1029_FRC-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_FRC-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_FRC-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1029_FRC-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 1751.608 sec; CPU: 22324.171 sec sorting block 160, #reads = 1022781 mem = 513333127 peak = 1292460032 uniq header count 1 merging 161 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 9813995253 output reads: 172664807 bam_mem_sort: 161 calls 60.830 user 0.404 sys 61.562 real bam_write: 161 calls 106.039 user 2.699 sys 109.600 real execute: 1 calls 34.198 user 1.652 sys 1806.460 real merge_files: 1 calls 34.185 user 1.628 sys 53.444 real parse_chunk: 2576 calls 78.655 user 23.256 sys 104.354 real read_chunk: 6962 calls 68.483 user 58.133 sys 690.502 real sort_block: 1 calls 51.973 user 57.517 sys 667.741 real write_chunk: 4384 calls 33.838 user 1.315 sys 52.428 real overall: 3578888192 mem 2062.587 user 101.507 sys 1806.486 real ls: cannot access '*.cram': No such file or directory