cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_BCAN-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1963.510 CPU sec, 78.208 real sec [M::process] read 872068 sequences (100000148 bp) in 1.152 CPU sec, 1.153 real sec... [M::process] read 870852 sequences (100000186 bp) in 1.678 CPU sec, 1.721 real sec... [M::process] read 867508 sequences (100000024 bp) in 1.648 CPU sec, 1.683 real sec... [M::process] read 866628 sequences (100000184 bp) in 1.691 CPU sec, 1.727 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 292914, 39, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 439, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3070) [M::mem_pestat] mean and std.dev: (630.54, 625.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.46, 44.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 719, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3808) [M::mem_pestat] mean and std.dev: (805.00, 838.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5024) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 478, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3156) [M::mem_pestat] mean and std.dev: (779.57, 679.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 292623, 57, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 446, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2581) [M::mem_pestat] mean and std.dev: (522.56, 496.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.68, 44.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 645, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3919) [M::mem_pestat] mean and std.dev: (839.65, 858.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 366, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2728) [M::mem_pestat] mean and std.dev: (587.57, 604.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 291782, 56, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 404, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2971) [M::mem_pestat] mean and std.dev: (617.57, 578.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.72, 45.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (255, 461, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3876) [M::mem_pestat] mean and std.dev: (682.67, 683.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 604, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3216) [M::mem_pestat] mean and std.dev: (820.05, 722.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 872068 reads in 570.970 CPU sec, 18.850 real sec [M::mem_process_seqs] Processed 870852 reads in 461.803 CPU sec, 15.357 real sec sorting block 0, #reads = 1047690 mem = 536878424 peak = 569864192 [M::process] read 864806 sequences (100000142 bp) in 2.314 CPU sec, 2.383 real sec... [M::mem_process_seqs] Processed 867508 reads in 525.817 CPU sec, 17.433 real sec sorting block 1, #reads = 1048325 mem = 536878529 peak = 696733696 [M::process] read 864284 sequences (100000059 bp) in 2.618 CPU sec, 2.726 real sec... [M::process] read 863362 sequences (100000220 bp) in 1.920 CPU sec, 1.925 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 292244, 52, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 475, 1288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3496) [M::mem_pestat] mean and std.dev: (654.01, 657.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.87, 45.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 814, 1457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3799) [M::mem_pestat] mean and std.dev: (843.06, 643.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4970) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 596, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3945) [M::mem_pestat] mean and std.dev: (823.05, 771.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 291194, 63, 93) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 377, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020) [M::mem_pestat] mean and std.dev: (653.68, 703.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.12, 44.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 494, 1673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4567) [M::mem_pestat] mean and std.dev: (937.87, 983.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 544, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3263) [M::mem_pestat] mean and std.dev: (695.82, 690.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 866628 reads in 413.559 CPU sec, 13.350 real sec sorting block 2, #reads = 1050028 mem = 536887411 peak = 834195456 [M::process] read 862462 sequences (100000058 bp) in 1.991 CPU sec, 2.007 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 291309, 64, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 354, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3591) [M::mem_pestat] mean and std.dev: (729.01, 831.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.35, 45.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (492, 991, 2236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5724) [M::mem_pestat] mean and std.dev: (1336.80, 1212.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 496, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3229) [M::mem_pestat] mean and std.dev: (677.69, 640.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 864284 reads in 246.529 CPU sec, 7.938 real sec sorting block 3, #reads = 1046870 mem = 536877849 peak = 834195456 [M::mem_process_seqs] Processed 864806 reads in 761.224 CPU sec, 24.556 real sec sorting block 4, #reads = 1049019 mem = 536887744 peak = 834195456 [M::process] read 861536 sequences (100000247 bp) in 2.500 CPU sec, 2.843 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 291075, 61, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 423, 1309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3561) [M::mem_pestat] mean and std.dev: (698.73, 746.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.59, 45.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 703, 1694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4632) [M::mem_pestat] mean and std.dev: (870.75, 826.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 564, 1232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3188) [M::mem_pestat] mean and std.dev: (689.21, 608.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4166) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 865780 sequences (100000018 bp) in 1.852 CPU sec, 1.860 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (103, 290260, 75, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 590, 1596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4422) [M::mem_pestat] mean and std.dev: (718.40, 647.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (125.07, 45.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (329, 1058, 1754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4604) [M::mem_pestat] mean and std.dev: (1104.90, 1000.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 460, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2717) [M::mem_pestat] mean and std.dev: (666.87, 577.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 291934, 60, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 533, 1391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3889) [M::mem_pestat] mean and std.dev: (770.03, 846.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.81, 45.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 667, 2018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5470) [M::mem_pestat] mean and std.dev: (1084.32, 1241.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 370, 1378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3802) [M::mem_pestat] mean and std.dev: (690.03, 721.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 863362 reads in 794.074 CPU sec, 25.527 real sec [M::mem_process_seqs] Processed 861536 reads in 447.094 CPU sec, 14.448 real sec sorting block 5, #reads = 1048868 mem = 536880663 peak = 834195456 [M::process] read 873158 sequences (100000058 bp) in 2.529 CPU sec, 2.603 real sec... [M::mem_process_seqs] Processed 862462 reads in 513.733 CPU sec, 16.722 real sec sorting block 6, #reads = 1050267 mem = 536884660 peak = 834195456 [M::process] read 871472 sequences (100000079 bp) in 2.418 CPU sec, 2.510 real sec... [M::process] read 868426 sequences (100000161 bp) in 1.610 CPU sec, 1.611 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 291996, 50, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 540, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3049) [M::mem_pestat] mean and std.dev: (696.55, 588.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (124.02, 45.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 516, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2711) [M::mem_pestat] mean and std.dev: (669.89, 629.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 354, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2665) [M::mem_pestat] mean and std.dev: (570.28, 521.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3501) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 293950, 56, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 424, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2828) [M::mem_pestat] mean and std.dev: (580.27, 553.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (122.28, 44.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 523, 1615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4379) [M::mem_pestat] mean and std.dev: (825.20, 880.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 473, 1152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3096) [M::mem_pestat] mean and std.dev: (663.43, 626.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 865780 reads in 394.361 CPU sec, 12.800 real sec sorting block 7, #reads = 1051366 mem = 536896409 peak = 834195456 [M::process] read 868348 sequences (100000143 bp) in 1.915 CPU sec, 1.921 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 294204, 54, 99) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 270, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3042) [M::mem_pestat] mean and std.dev: (588.84, 647.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (122.82, 44.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 476, 1646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4658) [M::mem_pestat] mean and std.dev: (811.78, 824.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 785, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4056) [M::mem_pestat] mean and std.dev: (993.51, 909.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 873158 reads in 448.654 CPU sec, 14.448 real sec sorting block 8, #reads = 1050620 mem = 536882618 peak = 834195456 [M::mem_process_seqs] Processed 871472 reads in 510.863 CPU sec, 16.458 real sec sorting block 9, #reads = 1052419 mem = 536884122 peak = 834195456 [M::process] read 866538 sequences (100000263 bp) in 2.930 CPU sec, 3.028 real sec... [M::process] read 865102 sequences (100000060 bp) in 1.848 CPU sec, 1.849 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 292765, 62, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 362, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2892) [M::mem_pestat] mean and std.dev: (575.38, 558.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.43, 44.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (176, 587, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3971) [M::mem_pestat] mean and std.dev: (895.36, 887.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 707, 1372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3622) [M::mem_pestat] mean and std.dev: (903.74, 832.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 293557, 58, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 375, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2410) [M::mem_pestat] mean and std.dev: (582.19, 517.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.54, 44.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 580, 1117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3009) [M::mem_pestat] mean and std.dev: (554.17, 482.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3955) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (347, 777, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3314) [M::mem_pestat] mean and std.dev: (903.48, 735.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 868348 reads in 383.703 CPU sec, 12.290 real sec sorting block 10, #reads = 1052315 mem = 536879752 peak = 834195456 [M::mem_process_seqs] Processed 868426 reads in 436.593 CPU sec, 14.054 real sec [M::process] read 863950 sequences (100000028 bp) in 2.599 CPU sec, 2.710 real sec... [M::process] read 862790 sequences (100000293 bp) in 1.610 CPU sec, 1.611 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 291572, 63, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 628, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (844.09, 700.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.07, 45.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (197, 659, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3497) [M::mem_pestat] mean and std.dev: (698.29, 652.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 408, 1147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3081) [M::mem_pestat] mean and std.dev: (755.40, 784.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 292209, 62, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 408, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2420) [M::mem_pestat] mean and std.dev: (560.55, 474.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.90, 45.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (334, 624, 1467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3733) [M::mem_pestat] mean and std.dev: (997.16, 895.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 727, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3524) [M::mem_pestat] mean and std.dev: (818.28, 699.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 865102 reads in 346.253 CPU sec, 11.094 real sec sorting block 11, #reads = 1055205 mem = 536882104 peak = 834195456 [M::mem_process_seqs] Processed 866538 reads in 406.818 CPU sec, 13.049 real sec sorting block 12, #reads = 1049181 mem = 536884371 peak = 888143872 [M::process] read 863486 sequences (100000062 bp) in 3.198 CPU sec, 3.289 real sec... [M::process] read 870052 sequences (100000028 bp) in 2.060 CPU sec, 2.058 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 291423, 76, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 433, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3814) [M::mem_pestat] mean and std.dev: (767.33, 844.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.45, 45.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (303, 727, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3648) [M::mem_pestat] mean and std.dev: (856.01, 822.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 471, 1679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4657) [M::mem_pestat] mean and std.dev: (895.41, 1072.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 291407, 66, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 405, 1208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3230) [M::mem_pestat] mean and std.dev: (646.01, 638.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.61, 45.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 556, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3970) [M::mem_pestat] mean and std.dev: (881.51, 978.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 379, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2249) [M::mem_pestat] mean and std.dev: (539.25, 458.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 863950 reads in 374.371 CPU sec, 11.980 real sec sorting block 13, #reads = 1050808 mem = 536880973 peak = 888143872 [M::mem_process_seqs] Processed 862790 reads in 436.829 CPU sec, 14.010 real sec sorting block 14, #reads = 1047263 mem = 536880106 peak = 888143872 [M::process] read 874506 sequences (100000237 bp) in 2.873 CPU sec, 2.986 real sec... [M::process] read 872348 sequences (100000142 bp) in 2.003 CPU sec, 2.008 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 291540, 55, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 577, 1309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3475) [M::mem_pestat] mean and std.dev: (817.05, 782.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.55, 45.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 463, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3294) [M::mem_pestat] mean and std.dev: (692.12, 719.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4313) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 525, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2825) [M::mem_pestat] mean and std.dev: (732.00, 657.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 292591, 49, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 376, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3214) [M::mem_pestat] mean and std.dev: (664.24, 724.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (122.94, 44.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (219, 492, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1797) [M::mem_pestat] mean and std.dev: (439.55, 331.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 518, 1298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3488) [M::mem_pestat] mean and std.dev: (780.94, 787.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 863486 reads in 401.189 CPU sec, 12.849 real sec sorting block 15, #reads = 1048395 mem = 536887636 peak = 888143872 [M::mem_process_seqs] Processed 870052 reads in 470.944 CPU sec, 15.110 real sec [M::process] read 869644 sequences (100000133 bp) in 3.448 CPU sec, 3.527 real sec... [M::process] read 868548 sequences (100000031 bp) in 2.229 CPU sec, 2.234 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 295069, 62, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 444, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2624) [M::mem_pestat] mean and std.dev: (577.44, 533.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (121.91, 43.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 556, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2746) [M::mem_pestat] mean and std.dev: (665.28, 630.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 388, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1878) [M::mem_pestat] mean and std.dev: (484.00, 392.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2439) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 294300, 55, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 475, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3413) [M::mem_pestat] mean and std.dev: (717.62, 700.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.40, 44.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (207, 661, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3069) [M::mem_pestat] mean and std.dev: (576.36, 532.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 500, 1517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4073) [M::mem_pestat] mean and std.dev: (851.75, 843.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5351) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 874506 reads in 395.127 CPU sec, 12.635 real sec sorting block 16, #reads = 1049466 mem = 536886133 peak = 888143872 [M::mem_process_seqs] Processed 872348 reads in 462.142 CPU sec, 14.810 real sec sorting block 17, #reads = 1053569 mem = 536887847 peak = 888143872 [M::process] read 866386 sequences (100000186 bp) in 3.076 CPU sec, 3.148 real sec... [M::process] read 864684 sequences (100000063 bp) in 2.098 CPU sec, 2.105 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 293758, 56, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 357, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012) [M::mem_pestat] mean and std.dev: (466.71, 436.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.05, 44.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 331, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2958) [M::mem_pestat] mean and std.dev: (549.96, 623.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 408, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505) [M::mem_pestat] mean and std.dev: (560.29, 469.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 293099, 53, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 436, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3411) [M::mem_pestat] mean and std.dev: (723.99, 783.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.37, 44.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 679, 2002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5642) [M::mem_pestat] mean and std.dev: (1210.89, 1329.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7462) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 579, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2296) [M::mem_pestat] mean and std.dev: (578.96, 420.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 869644 reads in 397.369 CPU sec, 12.712 real sec sorting block 18, #reads = 1053907 mem = 536884783 peak = 888143872 [M::mem_process_seqs] Processed 868548 reads in 460.417 CPU sec, 14.766 real sec sorting block 19, #reads = 1049643 mem = 536879233 peak = 888143872 [M::process] read 864076 sequences (100000078 bp) in 3.204 CPU sec, 3.279 real sec... [M::process] read 863474 sequences (100000150 bp) in 2.350 CPU sec, 2.353 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (96, 291593, 54, 98) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 505, 1233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3251) [M::mem_pestat] mean and std.dev: (741.44, 726.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (124.43, 45.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 698, 1366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3696) [M::mem_pestat] mean and std.dev: (839.78, 781.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 448, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3111) [M::mem_pestat] mean and std.dev: (681.81, 592.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 292249, 51, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 615, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4358) [M::mem_pestat] mean and std.dev: (875.17, 773.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.98, 45.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 673, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4768) [M::mem_pestat] mean and std.dev: (942.24, 911.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 458, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3222) [M::mem_pestat] mean and std.dev: (704.40, 740.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 864684 reads in 406.982 CPU sec, 13.029 real sec sorting block 20, #reads = 1050721 mem = 536884742 peak = 888143872 [M::mem_process_seqs] Processed 866386 reads in 469.927 CPU sec, 15.071 real sec [M::process] read 863546 sequences (100000151 bp) in 2.628 CPU sec, 2.703 real sec... sorting block 21, #reads = 1049944 mem = 536882616 peak = 888143872 [M::process] read 871230 sequences (100000176 bp) in 1.939 CPU sec, 1.945 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 291162, 71, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 402, 1059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2799) [M::mem_pestat] mean and std.dev: (672.48, 661.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.42, 45.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 564, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3353) [M::mem_pestat] mean and std.dev: (710.48, 661.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 438, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (600.32, 549.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 291217, 65, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 378, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3105) [M::mem_pestat] mean and std.dev: (551.76, 501.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.51, 45.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (230, 724, 1806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4958) [M::mem_pestat] mean and std.dev: (971.85, 1001.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6534) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 656, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3880) [M::mem_pestat] mean and std.dev: (779.41, 688.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 864076 reads in 411.110 CPU sec, 13.181 real sec [M::mem_process_seqs] Processed 863474 reads in 476.965 CPU sec, 15.310 real sec sorting block 22, #reads = 1047248 mem = 536896399 peak = 888143872 [M::process] read 873286 sequences (100000168 bp) in 3.099 CPU sec, 3.160 real sec... [M::process] read 871510 sequences (100000080 bp) in 2.318 CPU sec, 2.327 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 291502, 70, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 388, 1173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3191) [M::mem_pestat] mean and std.dev: (703.83, 732.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.47, 45.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 596, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270) [M::mem_pestat] mean and std.dev: (732.95, 673.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 745, 1467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3913) [M::mem_pestat] mean and std.dev: (836.07, 797.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 293449, 55, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 476, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3283) [M::mem_pestat] mean and std.dev: (770.41, 774.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.54, 44.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (159, 405, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3243) [M::mem_pestat] mean and std.dev: (627.51, 801.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 357, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2072) [M::mem_pestat] mean and std.dev: (510.19, 413.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 863546 reads in 413.025 CPU sec, 13.218 real sec sorting block 23, #reads = 1045165 mem = 536902488 peak = 888143872 [M::mem_process_seqs] Processed 871230 reads in 480.143 CPU sec, 15.403 real sec sorting block 24, #reads = 1042505 mem = 536879639 peak = 953126912 [M::process] read 869852 sequences (100000030 bp) in 2.987 CPU sec, 3.052 real sec... [M::process] read 868328 sequences (100000056 bp) in 2.335 CPU sec, 2.343 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 293348, 57, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 545, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3559) [M::mem_pestat] mean and std.dev: (742.20, 699.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.59, 44.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 660, 1907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5323) [M::mem_pestat] mean and std.dev: (1019.44, 1041.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7031) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 453, 1351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3707) [M::mem_pestat] mean and std.dev: (748.93, 881.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 294764, 55, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 366, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375) [M::mem_pestat] mean and std.dev: (587.33, 598.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.21, 44.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (227, 791, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4064) [M::mem_pestat] mean and std.dev: (932.51, 923.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 815, 1551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4325) [M::mem_pestat] mean and std.dev: (911.45, 843.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 871510 reads in 405.088 CPU sec, 12.979 real sec sorting block 25, #reads = 1054402 mem = 536886113 peak = 953126912 [M::mem_process_seqs] Processed 873286 reads in 468.805 CPU sec, 15.054 real sec [M::process] read 867456 sequences (100000161 bp) in 2.901 CPU sec, 2.983 real sec... sorting block 26, #reads = 1051902 mem = 536883195 peak = 953126912 [M::process] read 864186 sequences (100000114 bp) in 2.398 CPU sec, 2.401 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 293462, 76, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 434, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3025) [M::mem_pestat] mean and std.dev: (634.87, 653.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.00, 44.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 622, 1651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4333) [M::mem_pestat] mean and std.dev: (984.90, 974.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 354, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3251) [M::mem_pestat] mean and std.dev: (762.85, 792.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 293312, 53, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 877, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4575) [M::mem_pestat] mean and std.dev: (1112.77, 1041.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.37, 44.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (289, 746, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115) [M::mem_pestat] mean and std.dev: (830.86, 612.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 408, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4115) [M::mem_pestat] mean and std.dev: (764.20, 747.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 869852 reads in 412.423 CPU sec, 13.213 real sec sorting block 27, #reads = 1052827 mem = 536883832 peak = 953126912 [M::mem_process_seqs] Processed 868328 reads in 475.599 CPU sec, 15.267 real sec [M::process] read 863612 sequences (100000077 bp) in 2.961 CPU sec, 3.040 real sec... [M::process] read 863204 sequences (100000130 bp) in 2.315 CPU sec, 2.318 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 292736, 67, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 594, 1340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3444) [M::mem_pestat] mean and std.dev: (736.53, 748.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.62, 44.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 611, 1480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4030) [M::mem_pestat] mean and std.dev: (799.94, 782.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 567, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3082) [M::mem_pestat] mean and std.dev: (704.33, 635.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 291586, 72, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 567, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3300) [M::mem_pestat] mean and std.dev: (734.02, 709.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.18, 44.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 799, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4901) [M::mem_pestat] mean and std.dev: (1017.75, 1066.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 547, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2992) [M::mem_pestat] mean and std.dev: (732.21, 720.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 867456 reads in 407.997 CPU sec, 13.069 real sec sorting block 28, #reads = 1052450 mem = 536880852 peak = 953126912 [M::mem_process_seqs] Processed 864186 reads in 477.344 CPU sec, 15.407 real sec sorting block 29, #reads = 1048287 mem = 536894038 peak = 953126912 [M::process] read 863012 sequences (100000133 bp) in 3.189 CPU sec, 3.257 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 291420, 48, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 415, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3471) [M::mem_pestat] mean and std.dev: (715.83, 760.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.65, 45.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (304, 597, 1849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4939) [M::mem_pestat] mean and std.dev: (1049.48, 975.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (318, 739, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3192) [M::mem_pestat] mean and std.dev: (887.56, 781.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 875066 sequences (100000021 bp) in 2.296 CPU sec, 2.302 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 291400, 68, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 623, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744) [M::mem_pestat] mean and std.dev: (650.05, 566.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.62, 45.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (242, 646, 1760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4796) [M::mem_pestat] mean and std.dev: (954.17, 955.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 438, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2686) [M::mem_pestat] mean and std.dev: (652.25, 623.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (102, 290976, 64, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 542, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3333) [M::mem_pestat] mean and std.dev: (759.16, 705.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.70, 45.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 835, 2087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5687) [M::mem_pestat] mean and std.dev: (1209.28, 1246.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 761, 1724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4740) [M::mem_pestat] mean and std.dev: (1019.74, 1054.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 863612 reads in 571.867 CPU sec, 18.347 real sec sorting block 30, #reads = 1048995 mem = 536877463 peak = 953126912 [M::mem_process_seqs] Processed 863204 reads in 462.669 CPU sec, 14.951 real sec [M::mem_process_seqs] Processed 863012 reads in 532.070 CPU sec, 17.172 real sec sorting block 31, #reads = 1045737 mem = 536879292 peak = 953126912 [M::process] read 872878 sequences (100000052 bp) in 3.172 CPU sec, 3.258 real sec... sorting block 32, #reads = 1046971 mem = 536878061 peak = 953126912 [M::process] read 870744 sequences (100000186 bp) in 2.661 CPU sec, 2.775 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 293411, 68, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 445, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3209) [M::mem_pestat] mean and std.dev: (654.36, 608.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (121.74, 43.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 740, 1890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5158) [M::mem_pestat] mean and std.dev: (985.76, 1012.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 423, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (698.58, 676.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 869718 sequences (100000019 bp) in 2.095 CPU sec, 2.104 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 294071, 47, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 362, 1877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5331) [M::mem_pestat] mean and std.dev: (962.72, 1114.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.22, 43.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 486, 1603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4363) [M::mem_pestat] mean and std.dev: (921.02, 1081.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 414, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2497) [M::mem_pestat] mean and std.dev: (571.27, 536.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 294354, 53, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 490, 1146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2936) [M::mem_pestat] mean and std.dev: (763.73, 740.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.64, 43.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 649, 1683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4663) [M::mem_pestat] mean and std.dev: (1072.63, 1136.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 488, 1281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301) [M::mem_pestat] mean and std.dev: (726.52, 704.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 875066 reads in 583.778 CPU sec, 18.773 real sec [M::mem_process_seqs] Processed 872878 reads in 476.662 CPU sec, 15.473 real sec sorting block 33, #reads = 1042820 mem = 536878898 peak = 953126912 [M::mem_process_seqs] Processed 870744 reads in 543.876 CPU sec, 17.642 real sec [M::process] read 867892 sequences (100000253 bp) in 2.781 CPU sec, 2.873 real sec... sorting block 34, #reads = 1050383 mem = 536890642 peak = 953126912 [M::process] read 865694 sequences (100000102 bp) in 3.029 CPU sec, 3.124 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 293470, 62, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 462, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2599) [M::mem_pestat] mean and std.dev: (586.35, 535.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.04, 44.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 613, 1272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264) [M::mem_pestat] mean and std.dev: (778.95, 683.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 599, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3032) [M::mem_pestat] mean and std.dev: (744.10, 706.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 865052 sequences (100000062 bp) in 2.364 CPU sec, 2.371 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 292627, 53, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 427, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2234) [M::mem_pestat] mean and std.dev: (449.91, 361.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.42, 44.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (353, 650, 1480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3734) [M::mem_pestat] mean and std.dev: (804.64, 744.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 423, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3184) [M::mem_pestat] mean and std.dev: (698.40, 707.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 291512, 74, 103) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 585, 1286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392) [M::mem_pestat] mean and std.dev: (817.03, 747.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (124.05, 45.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 655, 1555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3911) [M::mem_pestat] mean and std.dev: (967.21, 822.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5089) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 438, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2898) [M::mem_pestat] mean and std.dev: (637.43, 558.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 869718 reads in 604.890 CPU sec, 19.460 real sec sorting block 35, #reads = 1051632 mem = 536885478 peak = 971894784 [M::mem_process_seqs] Processed 867892 reads in 492.199 CPU sec, 15.886 real sec sorting block 36, #reads = 1049819 mem = 536881598 peak = 971894784 [M::mem_process_seqs] Processed 865694 reads in 567.224 CPU sec, 18.287 real sec [M::process] read 863926 sequences (100000097 bp) in 2.847 CPU sec, 2.925 real sec... sorting block 37, #reads = 1045317 mem = 536881542 peak = 971894784 [M::process] read 864432 sequences (100000210 bp) in 2.577 CPU sec, 2.671 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 291287, 54, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 610, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3218) [M::mem_pestat] mean and std.dev: (723.28, 694.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.03, 44.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 590, 1484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3934) [M::mem_pestat] mean and std.dev: (941.02, 930.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5159) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 497, 1333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3669) [M::mem_pestat] mean and std.dev: (696.80, 665.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 868358 sequences (100000169 bp) in 2.398 CPU sec, 2.406 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 290787, 56, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 487, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2926) [M::mem_pestat] mean and std.dev: (692.90, 656.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.49, 45.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 559, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2572) [M::mem_pestat] mean and std.dev: (612.10, 539.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 371, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2554) [M::mem_pestat] mean and std.dev: (589.68, 581.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 291182, 63, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 474, 1185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3061) [M::mem_pestat] mean and std.dev: (690.45, 687.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.35, 45.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 583, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4686) [M::mem_pestat] mean and std.dev: (954.82, 1026.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 687, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3705) [M::mem_pestat] mean and std.dev: (833.27, 708.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 865052 reads in 599.140 CPU sec, 19.277 real sec sorting block 38, #reads = 1042415 mem = 536879360 peak = 971894784 [M::mem_process_seqs] Processed 863926 reads in 489.025 CPU sec, 15.794 real sec [M::process] read 879258 sequences (100000071 bp) in 3.180 CPU sec, 3.261 real sec... [M::process] read 875444 sequences (100000176 bp) in 2.408 CPU sec, 2.414 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 291190, 58, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 683, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3728) [M::mem_pestat] mean and std.dev: (904.71, 817.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.23, 44.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (546, 959, 2111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5241) [M::mem_pestat] mean and std.dev: (1116.36, 985.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 416, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (577.42, 498.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 868358 reads in 257.947 CPU sec, 8.264 real sec sorting block 39, #reads = 1043479 mem = 536896545 peak = 971894784 [M::mem_process_seqs] Processed 864432 reads in 877.829 CPU sec, 28.285 real sec sorting block 40, #reads = 1039858 mem = 536890512 peak = 1030926336 [M::process] read 872836 sequences (100000000 bp) in 2.705 CPU sec, 3.133 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 295028, 44, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 767, 1678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4546) [M::mem_pestat] mean and std.dev: (992.00, 972.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 113, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (120.74, 42.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 509, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3818) [M::mem_pestat] mean and std.dev: (772.07, 810.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 498, 1065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (614.23, 550.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 872408 sequences (100000085 bp) in 2.116 CPU sec, 2.122 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 294188, 58, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 393, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2502) [M::mem_pestat] mean and std.dev: (565.52, 579.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.30, 43.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 648, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917) [M::mem_pestat] mean and std.dev: (617.42, 551.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 431, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3534) [M::mem_pestat] mean and std.dev: (677.51, 719.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 293937, 57, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 584, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5013) [M::mem_pestat] mean and std.dev: (1153.95, 1331.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (121.73, 43.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 494, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4284) [M::mem_pestat] mean and std.dev: (814.38, 995.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5654) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 549, 1059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2711) [M::mem_pestat] mean and std.dev: (656.88, 568.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 879258 reads in 678.705 CPU sec, 21.831 real sec sorting block 41, #reads = 1042763 mem = 536883576 peak = 1030926336 [M::mem_process_seqs] Processed 872836 reads in 496.063 CPU sec, 15.993 real sec sorting block 42, #reads = 1037114 mem = 536883795 peak = 1030926336 [M::mem_process_seqs] Processed 875444 reads in 573.243 CPU sec, 18.456 real sec [M::process] read 870680 sequences (100000261 bp) in 2.868 CPU sec, 2.947 real sec... sorting block 43, #reads = 1042254 mem = 536889386 peak = 1030926336 [M::process] read 867642 sequences (100000132 bp) in 2.765 CPU sec, 2.858 real sec... [M::process] read 865708 sequences (100000115 bp) in 2.200 CPU sec, 2.201 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 293556, 53, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 488, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3395) [M::mem_pestat] mean and std.dev: (744.95, 682.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.43, 43.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 612, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4092) [M::mem_pestat] mean and std.dev: (871.42, 783.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 351, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2543) [M::mem_pestat] mean and std.dev: (631.16, 649.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3320) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 293069, 54, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 535, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3377) [M::mem_pestat] mean and std.dev: (776.74, 692.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.65, 44.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 673, 1475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3967) [M::mem_pestat] mean and std.dev: (895.87, 909.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 552, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2777) [M::mem_pestat] mean and std.dev: (658.95, 603.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (96, 291650, 49, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 346, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2000) [M::mem_pestat] mean and std.dev: (428.64, 379.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.41, 44.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 432, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3998) [M::mem_pestat] mean and std.dev: (861.17, 958.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5256) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 418, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2745) [M::mem_pestat] mean and std.dev: (583.86, 480.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 872408 reads in 566.511 CPU sec, 18.189 real sec sorting block 44, #reads = 1044610 mem = 536884293 peak = 1030926336 [M::mem_process_seqs] Processed 870680 reads in 460.517 CPU sec, 14.879 real sec [M::process] read 864710 sequences (100000194 bp) in 2.554 CPU sec, 2.626 real sec... [M::mem_process_seqs] Processed 867642 reads in 532.194 CPU sec, 17.160 real sec sorting block 45, #reads = 1042309 mem = 536889693 peak = 1030926336 [M::process] read 863590 sequences (100000042 bp) in 2.839 CPU sec, 2.921 real sec... [M::process] read 874168 sequences (100000103 bp) in 2.140 CPU sec, 2.141 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (102, 291364, 48, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 502, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3381) [M::mem_pestat] mean and std.dev: (869.67, 905.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (123.94, 44.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 721, 1385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3705) [M::mem_pestat] mean and std.dev: (787.83, 662.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 659, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3760) [M::mem_pestat] mean and std.dev: (731.15, 642.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 290880, 61, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 542, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2421) [M::mem_pestat] mean and std.dev: (589.57, 501.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.01, 44.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 456, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013) [M::mem_pestat] mean and std.dev: (555.24, 595.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 492, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2923) [M::mem_pestat] mean and std.dev: (637.77, 560.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 865708 reads in 415.350 CPU sec, 13.296 real sec sorting block 46, #reads = 1041639 mem = 536897686 peak = 1030926336 [M::mem_process_seqs] Processed 864710 reads in 485.507 CPU sec, 15.603 real sec sorting block 47, #reads = 1043136 mem = 536884233 peak = 1030926336 [M::process] read 875524 sequences (100000154 bp) in 3.460 CPU sec, 3.555 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 292722, 41, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 314, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839) [M::mem_pestat] mean and std.dev: (401.41, 335.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (121.89, 43.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (297, 848, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4326) [M::mem_pestat] mean and std.dev: (975.44, 823.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5669) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 481, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3123) [M::mem_pestat] mean and std.dev: (759.59, 708.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 872204 sequences (100000156 bp) in 2.122 CPU sec, 2.122 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 291125, 63, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 475, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3786) [M::mem_pestat] mean and std.dev: (767.97, 850.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.48, 45.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 594, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4780) [M::mem_pestat] mean and std.dev: (934.18, 945.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 522, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2801) [M::mem_pestat] mean and std.dev: (645.97, 555.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3657) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 874168 reads in 427.596 CPU sec, 13.714 real sec sorting block 48, #reads = 1037657 mem = 536883666 peak = 1030926336 [M::mem_process_seqs] Processed 863590 reads in 497.438 CPU sec, 16.001 real sec [M::process] read 870712 sequences (100000273 bp) in 3.045 CPU sec, 3.155 real sec... sorting block 49, #reads = 1046032 mem = 536882497 peak = 1030926336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 294551, 56, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 769, 1728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4732) [M::mem_pestat] mean and std.dev: (1122.17, 1164.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (121.70, 43.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 765, 1416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3696) [M::mem_pestat] mean and std.dev: (860.81, 806.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 328, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2484) [M::mem_pestat] mean and std.dev: (498.56, 528.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 871208 sequences (100000219 bp) in 2.505 CPU sec, 2.513 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 294263, 48, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 600, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3448) [M::mem_pestat] mean and std.dev: (922.95, 937.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.53, 43.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 524, 1510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4106) [M::mem_pestat] mean and std.dev: (921.21, 990.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 419, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2937) [M::mem_pestat] mean and std.dev: (668.48, 623.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 875524 reads in 412.729 CPU sec, 13.224 real sec sorting block 50, #reads = 1048448 mem = 536883099 peak = 1030926336 [M::mem_process_seqs] Processed 872204 reads in 479.752 CPU sec, 15.416 real sec [M::process] read 869248 sequences (100000022 bp) in 2.940 CPU sec, 3.024 real sec... [M::process] read 868970 sequences (100000106 bp) in 2.091 CPU sec, 2.101 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 293563, 60, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 393, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3696) [M::mem_pestat] mean and std.dev: (820.60, 901.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.63, 43.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 555, 1052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2808) [M::mem_pestat] mean and std.dev: (646.81, 668.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 523, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2355) [M::mem_pestat] mean and std.dev: (613.81, 565.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 293884, 62, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 482, 1412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3886) [M::mem_pestat] mean and std.dev: (841.34, 899.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (122.59, 43.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 531, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3427) [M::mem_pestat] mean and std.dev: (730.00, 733.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 859, 1819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5065) [M::mem_pestat] mean and std.dev: (1116.64, 1163.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6688) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 870712 reads in 430.869 CPU sec, 13.809 real sec sorting block 51, #reads = 1053685 mem = 536886451 peak = 1030926336 [M::mem_process_seqs] Processed 871208 reads in 498.941 CPU sec, 16.043 real sec sorting block 52, #reads = 1045268 mem = 536884739 peak = 1037438976 [M::process] read 866674 sequences (100000206 bp) in 2.881 CPU sec, 2.967 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 293379, 52, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 368, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1928) [M::mem_pestat] mean and std.dev: (455.49, 402.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.18, 44.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (227, 600, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3761) [M::mem_pestat] mean and std.dev: (735.15, 741.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4939) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 450, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3353) [M::mem_pestat] mean and std.dev: (823.45, 837.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 865576 sequences (100000197 bp) in 2.369 CPU sec, 2.384 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 292795, 63, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 681, 1769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4691) [M::mem_pestat] mean and std.dev: (1000.49, 989.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.28, 44.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (207, 680, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3927) [M::mem_pestat] mean and std.dev: (890.44, 812.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 424, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1726) [M::mem_pestat] mean and std.dev: (450.65, 329.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 291604, 63, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 338, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2569) [M::mem_pestat] mean and std.dev: (556.44, 557.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.80, 44.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 459, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3614) [M::mem_pestat] mean and std.dev: (821.12, 889.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 500, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4457) [M::mem_pestat] mean and std.dev: (840.02, 870.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 869248 reads in 585.659 CPU sec, 18.800 real sec sorting block 53, #reads = 1051837 mem = 536884536 peak = 1037438976 [M::mem_process_seqs] Processed 868970 reads in 478.191 CPU sec, 15.465 real sec sorting block 54, #reads = 1047276 mem = 536883147 peak = 1037438976 [M::mem_process_seqs] Processed 866674 reads in 551.684 CPU sec, 17.816 real sec [M::process] read 864994 sequences (100000129 bp) in 2.846 CPU sec, 2.901 real sec... sorting block 55, #reads = 1044891 mem = 536879006 peak = 1037438976 [M::process] read 878094 sequences (100000230 bp) in 2.228 CPU sec, 2.339 real sec... [M::process] read 879250 sequences (100000036 bp) in 2.088 CPU sec, 2.107 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 291319, 60, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 368, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2595) [M::mem_pestat] mean and std.dev: (589.84, 535.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (123.93, 44.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 676, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3490) [M::mem_pestat] mean and std.dev: (811.57, 774.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 356, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2088) [M::mem_pestat] mean and std.dev: (514.68, 474.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 290581, 67, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 556, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3351) [M::mem_pestat] mean and std.dev: (743.89, 662.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (124.02, 44.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (170, 483, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4523) [M::mem_pestat] mean and std.dev: (826.65, 955.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 499, 1727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4781) [M::mem_pestat] mean and std.dev: (948.55, 1088.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 865576 reads in 419.612 CPU sec, 13.465 real sec sorting block 56, #reads = 1038309 mem = 536892559 peak = 1037438976 [M::process] read 876276 sequences (100000027 bp) in 2.757 CPU sec, 2.766 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 293139, 62, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 667, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3428) [M::mem_pestat] mean and std.dev: (787.65, 765.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (121.08, 42.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (196, 545, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3256) [M::mem_pestat] mean and std.dev: (684.46, 698.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4276) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 755, 1471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4019) [M::mem_pestat] mean and std.dev: (872.26, 859.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 864994 reads in 501.072 CPU sec, 16.111 real sec [M::mem_process_seqs] Processed 878094 reads in 583.017 CPU sec, 18.745 real sec sorting block 57, #reads = 1031285 mem = 536887058 peak = 1037438976 [M::process] read 874304 sequences (100000045 bp) in 3.089 CPU sec, 3.141 real sec... [M::process] read 872430 sequences (100000095 bp) in 2.081 CPU sec, 2.084 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 294508, 65, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 316, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2285) [M::mem_pestat] mean and std.dev: (461.62, 438.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 113, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (120.88, 42.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 496, 1571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4397) [M::mem_pestat] mean and std.dev: (874.09, 958.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 374, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2415) [M::mem_pestat] mean and std.dev: (594.44, 547.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 294975, 73, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 482, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2634) [M::mem_pestat] mean and std.dev: (583.56, 553.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (121.37, 42.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 849, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3829) [M::mem_pestat] mean and std.dev: (919.15, 754.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 453, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3470) [M::mem_pestat] mean and std.dev: (634.37, 643.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 879250 reads in 427.867 CPU sec, 13.720 real sec sorting block 58, #reads = 1032559 mem = 536901024 peak = 1037438976 [M::mem_process_seqs] Processed 876276 reads in 503.989 CPU sec, 16.187 real sec sorting block 59, #reads = 1039159 mem = 536882313 peak = 1037438976 [M::process] read 869760 sequences (100000085 bp) in 3.085 CPU sec, 3.148 real sec... [M::process] read 866624 sequences (100000124 bp) in 2.139 CPU sec, 2.141 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 292797, 48, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 474, 967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2469) [M::mem_pestat] mean and std.dev: (577.79, 508.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (122.17, 43.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 674, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4163) [M::mem_pestat] mean and std.dev: (843.42, 870.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5478) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 541, 1311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3509) [M::mem_pestat] mean and std.dev: (722.03, 665.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 293554, 50, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 388, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2299) [M::mem_pestat] mean and std.dev: (568.63, 540.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 114, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (121.84, 43.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 368, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3059) [M::mem_pestat] mean and std.dev: (502.19, 591.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4048) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 413, 1219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3297) [M::mem_pestat] mean and std.dev: (731.21, 743.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 872430 reads in 417.681 CPU sec, 13.372 real sec sorting block 60, #reads = 1040916 mem = 536882916 peak = 1037438976 [M::mem_process_seqs] Processed 874304 reads in 494.243 CPU sec, 15.858 real sec sorting block 61, #reads = 1044337 mem = 536884853 peak = 1037438976 [M::process] read 864144 sequences (100000124 bp) in 2.828 CPU sec, 2.889 real sec... [M::process] read 862460 sequences (100000210 bp) in 2.419 CPU sec, 2.423 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 291952, 55, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 522, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3104) [M::mem_pestat] mean and std.dev: (680.11, 559.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (123.83, 44.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 613, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2875) [M::mem_pestat] mean and std.dev: (647.17, 523.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 517, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3161) [M::mem_pestat] mean and std.dev: (733.40, 718.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 291793, 82, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 590, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3814) [M::mem_pestat] mean and std.dev: (808.27, 775.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (123.05, 44.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 468, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2969) [M::mem_pestat] mean and std.dev: (620.45, 620.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3903) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 322, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279) [M::mem_pestat] mean and std.dev: (526.50, 480.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 866624 reads in 411.681 CPU sec, 13.173 real sec sorting block 62, #reads = 1040966 mem = 536880084 peak = 1037438976 [M::process] read 770480 sequences (89165378 bp) in 2.400 CPU sec, 2.409 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 291059, 56, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 570, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3820) [M::mem_pestat] mean and std.dev: (923.98, 875.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.37, 45.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (198, 453, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2520) [M::mem_pestat] mean and std.dev: (565.75, 483.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3294) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 430, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3651) [M::mem_pestat] mean and std.dev: (766.60, 752.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 864144 reads in 231.476 CPU sec, 7.435 real sec [M::mem_process_seqs] Processed 869760 reads in 766.269 CPU sec, 24.586 real sec sorting block 63, #reads = 1045065 mem = 536879100 peak = 1037438976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 290597, 45, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 313, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2520) [M::mem_pestat] mean and std.dev: (513.48, 532.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (125.01, 45.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 512, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2510) [M::mem_pestat] mean and std.dev: (560.71, 475.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 666, 1369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3601) [M::mem_pestat] mean and std.dev: (877.06, 755.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 259677, 56, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 294, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1306) [M::mem_pestat] mean and std.dev: (296.31, 231.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (124.40, 45.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 486, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4120) [M::mem_pestat] mean and std.dev: (800.28, 832.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 392, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2285) [M::mem_pestat] mean and std.dev: (603.56, 582.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 862460 reads in 602.784 CPU sec, 19.440 real sec sorting block 64, #reads = 1040791 mem = 536883248 peak = 1037438976 [M::mem_process_seqs] Processed 770480 reads in 274.000 CPU sec, 8.887 real sec sorting block 65, #reads = 1043370 mem = 536892626 peak = 1037438976 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 67644632 overall: 77143658496 mem 20049.078 user 463.044 sys 684.524 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_BCAN-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1029_BCAN-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_BCAN-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_BCAN-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1029_BCAN-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 684.588 sec; CPU: 20512.150 sec sorting block 66, #reads = 481653 mem = 248967039 peak = 1037438976 uniq header count 1 merging 67 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 5097779655 output reads: 69555430 bam_mem_sort: 67 calls 41.428 user 1.000 sys 42.661 real bam_write: 67 calls 36.521 user 2.231 sys 47.489 real execute: 1 calls 20.832 user 1.144 sys 711.976 real merge_files: 1 calls 20.830 user 1.102 sys 26.982 real parse_chunk: 1064 calls 46.336 user 11.658 sys 60.221 real read_chunk: 2948 calls 24.542 user 20.549 sys 598.310 real sort_block: 1 calls 16.017 user 20.065 sys 586.757 real write_chunk: 1882 calls 20.465 user 0.861 sys 26.021 real overall: 4334686208 mem 1045.418 user 52.084 sys 711.999 real ls: cannot access '*.cram': No such file or directory