.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_cfy-T1-TDNA-1_A23MHWWLT4_1;L008;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e1/a8007acfb36f0d6d4c49ca5d66a17a/.command.err
Size: 212.3 KB
Task: DAQ:SENTIEON_BWAMEM (659_cfy-T1-TDNA-1_A23MHWWLT4_1;L008;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cfy-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1927.932 CPU sec, 68.570 real sec
[M::process] read 686488 sequences (100000055 bp) in 1.341 CPU sec, 1.344 real sec...
[M::process] read 682354 sequences (100000229 bp) in 1.625 CPU sec, 1.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286713, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (297.70, 141.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683360 sequences (100000264 bp) in 1.498 CPU sec, 1.536 real sec...
[M::process] read 683262 sequences (100000035 bp) in 1.487 CPU sec, 1.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284985, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 299, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (317.34, 147.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686488 reads in 173.263 CPU sec, 5.527 real sec
[M::mem_process_seqs] Processed 682354 reads in 98.269 CPU sec, 3.162 real sec
sorting block 0, #reads = 1086853 mem = 536878142 peak = 586387456
[M::process] read 685630 sequences (100000029 bp) in 1.778 CPU sec, 1.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286000, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 295, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (313.60, 146.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685352 sequences (100000130 bp) in 1.628 CPU sec, 1.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285011, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (314.33, 147.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286084, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.46, 144.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683360 reads in 213.845 CPU sec, 6.873 real sec
[M::mem_process_seqs] Processed 683262 reads in 161.459 CPU sec, 5.214 real sec
sorting block 1, #reads = 1084980 mem = 536878849 peak = 715362304
[M::process] read 685588 sequences (100000029 bp) in 1.678 CPU sec, 1.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286883, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.39, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685352 reads in 77.985 CPU sec, 2.525 real sec
[M::process] read 685818 sequences (100000265 bp) in 1.552 CPU sec, 1.600 real sec...
sorting block 2, #reads = 1086117 mem = 536878231 peak = 822075392
[M::mem_process_seqs] Processed 685630 reads in 298.059 CPU sec, 9.644 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286110, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (302.48, 143.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684598 sequences (100000004 bp) in 1.891 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286452, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (300.67, 143.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685588 reads in 160.698 CPU sec, 5.123 real sec
[M::mem_process_seqs] Processed 685818 reads in 99.708 CPU sec, 3.181 real sec
sorting block 3, #reads = 1086894 mem = 536878902 peak = 828854272
[M::process] read 681954 sequences (100000053 bp) in 1.943 CPU sec, 1.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285164, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 286, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (306.61, 145.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1087434 mem = 536878440 peak = 888602624
[M::process] read 684316 sequences (100000037 bp) in 2.225 CPU sec, 2.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285092, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (320.98, 149.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684598 reads in 151.667 CPU sec, 4.877 real sec
[M::mem_process_seqs] Processed 681954 reads in 98.846 CPU sec, 3.156 real sec
[M::process] read 685876 sequences (100000211 bp) in 1.763 CPU sec, 1.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285529, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (309.99, 146.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1086517 mem = 536879450 peak = 888602624
[M::process] read 684764 sequences (100000250 bp) in 2.379 CPU sec, 2.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285976, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (301.39, 143.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684316 reads in 153.039 CPU sec, 4.941 real sec
[M::mem_process_seqs] Processed 685876 reads in 102.609 CPU sec, 3.278 real sec
[M::process] read 684716 sequences (100000141 bp) in 1.955 CPU sec, 2.087 real sec...
sorting block 6, #reads = 1087591 mem = 536879468 peak = 888602624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285743, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (305.48, 145.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684982 sequences (100000254 bp) in 1.989 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286197, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (304.81, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684764 reads in 155.376 CPU sec, 5.028 real sec
[M::process] read 684700 sequences (100000181 bp) in 1.808 CPU sec, 1.851 real sec...
[M::mem_process_seqs] Processed 684716 reads in 102.105 CPU sec, 3.264 real sec
sorting block 7, #reads = 1087940 mem = 536880823 peak = 888602624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285603, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (304.65, 144.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683036 sequences (100000207 bp) in 2.501 CPU sec, 2.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285725, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (302.43, 143.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684982 reads in 156.162 CPU sec, 5.032 real sec
sorting block 8, #reads = 1088091 mem = 536880073 peak = 888602624
[M::mem_process_seqs] Processed 684700 reads in 102.688 CPU sec, 3.257 real sec
[M::process] read 682928 sequences (100000186 bp) in 1.931 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285121, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 290, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (310.80, 145.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1087061 mem = 536879437 peak = 896356352
[M::process] read 684738 sequences (100000258 bp) in 2.145 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285492, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 292, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (311.63, 145.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683036 reads in 154.665 CPU sec, 5.041 real sec
[M::mem_process_seqs] Processed 682928 reads in 101.816 CPU sec, 3.309 real sec
[M::process] read 684886 sequences (100000278 bp) in 1.919 CPU sec, 2.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285425, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 279, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (300.63, 141.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 10, #reads = 1084877 mem = 536883186 peak = 896356352
[M::process] read 682552 sequences (100000148 bp) in 2.488 CPU sec, 2.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285865, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (301.12, 142.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684738 reads in 153.495 CPU sec, 5.155 real sec
[M::mem_process_seqs] Processed 684886 reads in 102.278 CPU sec, 3.521 real sec
[M::process] read 684194 sequences (100000182 bp) in 2.093 CPU sec, 2.453 real sec...
sorting block 11, #reads = 1086474 mem = 536879276 peak = 896356352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285476, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 294, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (313.05, 146.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683814 sequences (100000225 bp) in 2.087 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286093, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (302.19, 141.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682552 reads in 152.010 CPU sec, 4.996 real sec
[M::mem_process_seqs] Processed 684194 reads in 101.395 CPU sec, 3.323 real sec
sorting block 12, #reads = 1087197 mem = 536879778 peak = 896356352
[M::process] read 684216 sequences (100000231 bp) in 1.975 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285612, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (303.47, 141.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683142 sequences (100000080 bp) in 2.355 CPU sec, 2.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285239, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (301.43, 140.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683814 reads in 152.912 CPU sec, 5.073 real sec
sorting block 13, #reads = 1087464 mem = 536879542 peak = 896356352
[M::mem_process_seqs] Processed 684216 reads in 102.215 CPU sec, 3.289 real sec
[M::process] read 682112 sequences (100000087 bp) in 2.128 CPU sec, 2.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285548, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 288, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (307.48, 143.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1086488 mem = 536879463 peak = 896356352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284814, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 683610 sequences (100000260 bp) in 2.129 CPU sec, 2.612 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (199, 293, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (311.77, 143.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683142 reads in 153.588 CPU sec, 5.109 real sec
[M::mem_process_seqs] Processed 682112 reads in 100.840 CPU sec, 3.353 real sec
[M::process] read 684300 sequences (100000097 bp) in 2.329 CPU sec, 2.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285706, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 284, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (303.58, 140.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1086612 mem = 536878781 peak = 896356352
[M::process] read 684406 sequences (100000147 bp) in 2.231 CPU sec, 2.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286042, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (301.27, 140.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683610 reads in 151.917 CPU sec, 4.980 real sec
[M::mem_process_seqs] Processed 684300 reads in 98.430 CPU sec, 3.251 real sec
[M::process] read 682742 sequences (100000160 bp) in 2.047 CPU sec, 2.210 real sec...
sorting block 16, #reads = 1086501 mem = 536879281 peak = 896356352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285719, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (301.17, 140.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683952 sequences (100000102 bp) in 2.258 CPU sec, 2.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285121, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 290, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (308.69, 142.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684406 reads in 151.180 CPU sec, 4.964 real sec
[M::mem_process_seqs] Processed 682742 reads in 99.264 CPU sec, 3.216 real sec
sorting block 17, #reads = 1087046 mem = 536879369 peak = 896356352
[M::process] read 684036 sequences (100000100 bp) in 2.367 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285757, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (303.78, 142.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285691, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (305.39, 144.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683812 sequences (100000006 bp) in 2.395 CPU sec, 3.134 real sec...
[M::mem_process_seqs] Processed 683952 reads in 155.240 CPU sec, 5.068 real sec
sorting block 18, #reads = 1085398 mem = 536880009 peak = 896356352
[M::mem_process_seqs] Processed 684036 reads in 104.855 CPU sec, 3.389 real sec
[M::process] read 683460 sequences (100000071 bp) in 2.500 CPU sec, 2.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285182, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.61, 144.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1086815 mem = 536879622 peak = 906366976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285567, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 288, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (309.31, 145.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683364 sequences (100000264 bp) in 2.579 CPU sec, 2.862 real sec...
[M::mem_process_seqs] Processed 683812 reads in 158.202 CPU sec, 5.170 real sec
[M::mem_process_seqs] Processed 683460 reads in 103.529 CPU sec, 3.421 real sec
[M::process] read 685546 sequences (100000002 bp) in 2.250 CPU sec, 2.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286134, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 293, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (312.70, 146.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1086484 mem = 536879933 peak = 906366976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286479, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (301.71, 142.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684770 sequences (100000100 bp) in 2.394 CPU sec, 2.607 real sec...
[M::mem_process_seqs] Processed 683364 reads in 156.629 CPU sec, 5.092 real sec
[M::mem_process_seqs] Processed 685546 reads in 99.879 CPU sec, 3.254 real sec
sorting block 21, #reads = 1085872 mem = 536878078 peak = 906366976
[M::process] read 683492 sequences (100000067 bp) in 2.431 CPU sec, 2.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286122, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (303.19, 141.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285965, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (312.52, 146.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684510 sequences (100000077 bp) in 2.251 CPU sec, 2.690 real sec...
[M::mem_process_seqs] Processed 684770 reads in 155.643 CPU sec, 5.093 real sec
[M::mem_process_seqs] Processed 683492 reads in 101.694 CPU sec, 3.336 real sec
sorting block 22, #reads = 1088200 mem = 536879348 peak = 906366976
[M::process] read 684464 sequences (100000161 bp) in 2.212 CPU sec, 2.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285567, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (307.70, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684510 reads in 86.649 CPU sec, 3.011 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285644, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (307.54, 145.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684342 sequences (100000077 bp) in 2.407 CPU sec, 2.781 real sec...
sorting block 23, #reads = 1087163 mem = 536884600 peak = 906366976
[M::mem_process_seqs] Processed 684464 reads in 87.050 CPU sec, 3.089 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285745, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 289, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (308.69, 146.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684108 sequences (100000104 bp) in 2.552 CPU sec, 2.823 real sec...
[M::mem_process_seqs] Processed 684342 reads in 84.914 CPU sec, 2.926 real sec
sorting block 24, #reads = 1087480 mem = 536878227 peak = 906366976
[M::process] read 685030 sequences (100000199 bp) in 2.241 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285351, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (308.33, 144.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286039, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 285, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (304.37, 143.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684108 reads in 154.658 CPU sec, 5.116 real sec
[M::process] read 684720 sequences (100000039 bp) in 2.547 CPU sec, 3.083 real sec...
[M::mem_process_seqs] Processed 685030 reads in 100.548 CPU sec, 3.315 real sec
sorting block 25, #reads = 1088033 mem = 536876717 peak = 906366976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285934, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (306.10, 144.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682736 sequences (100000042 bp) in 2.424 CPU sec, 2.663 real sec...
[M::mem_process_seqs] Processed 684720 reads in 85.925 CPU sec, 3.016 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285174, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 297, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (314.32, 145.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1088376 mem = 536886635 peak = 906366976
[M::process] read 684202 sequences (100000222 bp) in 2.035 CPU sec, 2.362 real sec...
[M::mem_process_seqs] Processed 682736 reads in 82.830 CPU sec, 2.977 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285851, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (308.41, 145.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684834 sequences (100000104 bp) in 2.194 CPU sec, 2.523 real sec...
[M::mem_process_seqs] Processed 684202 reads in 86.137 CPU sec, 3.042 real sec
sorting block 27, #reads = 1087892 mem = 536880661 peak = 906366976
[M::process] read 684548 sequences (100000242 bp) in 2.178 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285927, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (305.12, 143.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285660, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (306.95, 144.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684380 sequences (100000089 bp) in 2.267 CPU sec, 2.647 real sec...
[M::mem_process_seqs] Processed 684834 reads in 157.051 CPU sec, 5.183 real sec
sorting block 28, #reads = 1087681 mem = 536877876 peak = 906366976
[M::mem_process_seqs] Processed 684548 reads in 99.490 CPU sec, 3.276 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285439, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (305.91, 143.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684370 sequences (100000195 bp) in 2.253 CPU sec, 2.594 real sec...
[M::mem_process_seqs] Processed 684380 reads in 86.366 CPU sec, 2.985 real sec
sorting block 29, #reads = 1087840 mem = 536879747 peak = 930512896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285703, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (306.16, 144.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684076 sequences (100000118 bp) in 2.143 CPU sec, 2.634 real sec...
[M::mem_process_seqs] Processed 684370 reads in 85.916 CPU sec, 3.094 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285553, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.67, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682558 sequences (100000272 bp) in 2.363 CPU sec, 2.697 real sec...
[M::mem_process_seqs] Processed 684076 reads in 84.967 CPU sec, 2.953 real sec
sorting block 30, #reads = 1088612 mem = 536879696 peak = 930512896
[M::process] read 681506 sequences (100000079 bp) in 2.133 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285411, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 293, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (311.60, 144.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682558 reads in 85.495 CPU sec, 3.051 real sec
sorting block 31, #reads = 1087505 mem = 536879830 peak = 930512896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284464, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (318.41, 147.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683806 sequences (100000300 bp) in 2.336 CPU sec, 2.639 real sec...
[M::mem_process_seqs] Processed 681506 reads in 83.247 CPU sec, 2.946 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285687, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (305.18, 142.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684162 sequences (100000177 bp) in 2.049 CPU sec, 2.434 real sec...
[M::mem_process_seqs] Processed 683806 reads in 85.619 CPU sec, 3.120 real sec
sorting block 32, #reads = 1086789 mem = 536877547 peak = 930512896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285823, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.33, 141.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684030 sequences (100000098 bp) in 2.488 CPU sec, 2.894 real sec...
[M::mem_process_seqs] Processed 684162 reads in 87.926 CPU sec, 3.052 real sec
[M::process] read 684650 sequences (100000088 bp) in 2.224 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285607, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.62, 144.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1086508 mem = 536879304 peak = 935149568
[M::mem_process_seqs] Processed 684030 reads in 83.560 CPU sec, 2.986 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285927, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (303.76, 143.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684334 sequences (100000195 bp) in 2.682 CPU sec, 3.100 real sec...
[M::mem_process_seqs] Processed 684650 reads in 88.714 CPU sec, 3.116 real sec
sorting block 34, #reads = 1087032 mem = 536880440 peak = 935149568
[M::process] read 684140 sequences (100000233 bp) in 2.134 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285698, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (304.34, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684334 reads in 86.248 CPU sec, 3.035 real sec
[M::process] read 683656 sequences (100000207 bp) in 2.338 CPU sec, 2.765 real sec...
sorting block 35, #reads = 1087429 mem = 536880107 peak = 935149568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285871, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (301.27, 140.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285477, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.39, 141.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681832 sequences (100000071 bp) in 2.423 CPU sec, 2.781 real sec...
[M::mem_process_seqs] Processed 684140 reads in 157.354 CPU sec, 5.290 real sec
[M::mem_process_seqs] Processed 683656 reads in 103.153 CPU sec, 3.433 real sec
sorting block 36, #reads = 1086997 mem = 536878547 peak = 935149568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284300, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 295, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (313.37, 144.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683984 sequences (100000166 bp) in 2.181 CPU sec, 2.658 real sec...
[M::mem_process_seqs] Processed 681832 reads in 84.226 CPU sec, 3.006 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285857, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 284, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.22, 141.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684410 sequences (100000255 bp) in 2.393 CPU sec, 2.712 real sec...
[M::mem_process_seqs] Processed 683984 reads in 89.506 CPU sec, 3.091 real sec
sorting block 37, #reads = 1087230 mem = 536877303 peak = 935149568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285968, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (266, 266, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 638)
[M::mem_pestat] mean and std.dev: (274.89, 98.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (302.02, 141.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684426 sequences (100000127 bp) in 2.241 CPU sec, 2.487 real sec...
[M::mem_process_seqs] Processed 684410 reads in 85.966 CPU sec, 2.975 real sec
sorting block 38, #reads = 1086154 mem = 536880873 peak = 978415616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285927, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (301.81, 141.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684282 sequences (100000152 bp) in 2.592 CPU sec, 3.018 real sec...
[M::mem_process_seqs] Processed 684426 reads in 87.931 CPU sec, 3.062 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285699, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (302.31, 141.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684252 sequences (100000063 bp) in 2.358 CPU sec, 2.795 real sec...
[M::mem_process_seqs] Processed 684282 reads in 87.288 CPU sec, 3.106 real sec
sorting block 39, #reads = 1086535 mem = 536883432 peak = 978415616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286070, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.76, 141.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684520 sequences (100000203 bp) in 2.214 CPU sec, 2.558 real sec...
[M::mem_process_seqs] Processed 684252 reads in 86.480 CPU sec, 2.997 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285421, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (303.27, 143.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1086437 mem = 536877978 peak = 978415616
[M::process] read 684534 sequences (100000263 bp) in 2.502 CPU sec, 2.978 real sec...
[M::mem_process_seqs] Processed 684520 reads in 88.071 CPU sec, 3.059 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285946, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (304.85, 144.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684450 sequences (100000263 bp) in 2.313 CPU sec, 2.643 real sec...
[M::mem_process_seqs] Processed 684534 reads in 88.004 CPU sec, 3.037 real sec
sorting block 41, #reads = 1086866 mem = 536878984 peak = 978415616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285770, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (302.54, 142.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685346 sequences (100000248 bp) in 2.403 CPU sec, 2.729 real sec...
[M::mem_process_seqs] Processed 684450 reads in 90.433 CPU sec, 3.188 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286505, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (301.79, 142.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685960 sequences (100000094 bp) in 2.396 CPU sec, 2.671 real sec...
sorting block 42, #reads = 1086060 mem = 536877844 peak = 978415616
[M::mem_process_seqs] Processed 685346 reads in 93.069 CPU sec, 3.147 real sec
[M::process] read 685398 sequences (100000146 bp) in 2.503 CPU sec, 2.798 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286508, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 281, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (299.27, 139.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685960 reads in 92.782 CPU sec, 3.173 real sec
sorting block 43, #reads = 1085485 mem = 536878257 peak = 978415616
[M::process] read 685472 sequences (100000081 bp) in 2.367 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286318, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (299.32, 138.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685398 reads in 90.700 CPU sec, 3.204 real sec
[M::process] read 685236 sequences (100000279 bp) in 2.466 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285912, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (300.72, 140.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1085582 mem = 536878873 peak = 978415616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286002, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (301.52, 139.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684578 sequences (100000161 bp) in 2.166 CPU sec, 2.516 real sec...
[M::mem_process_seqs] Processed 685472 reads in 164.329 CPU sec, 5.382 real sec
[M::mem_process_seqs] Processed 685236 reads in 105.085 CPU sec, 3.420 real sec
sorting block 45, #reads = 1086109 mem = 536881598 peak = 978415616
[M::process] read 684270 sequences (100000043 bp) in 2.253 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286135, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (302.95, 139.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683040 sequences (100000006 bp) in 2.355 CPU sec, 2.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285968, 3, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (307.50, 143.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 268, 1066)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2662)
[M::mem_pestat] mean and std.dev: (528.40, 382.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3460)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684578 reads in 158.828 CPU sec, 5.180 real sec
[M::mem_process_seqs] Processed 684270 reads in 102.576 CPU sec, 3.358 real sec
sorting block 46, #reads = 1085954 mem = 536878804 peak = 978415616
[M::process] read 682044 sequences (100000219 bp) in 2.026 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285046, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (317.01, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683040 reads in 87.349 CPU sec, 3.042 real sec
[M::process] read 684912 sequences (100000224 bp) in 2.655 CPU sec, 2.899 real sec...
sorting block 47, #reads = 1086729 mem = 536877078 peak = 978415616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285422, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (318.24, 145.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682044 reads in 88.700 CPU sec, 3.071 real sec
[M::process] read 685154 sequences (100000239 bp) in 2.175 CPU sec, 2.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286345, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (302.57, 140.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684912 reads in 86.981 CPU sec, 3.091 real sec
sorting block 48, #reads = 1085733 mem = 536879375 peak = 978415616
[M::process] read 684984 sequences (100000098 bp) in 2.369 CPU sec, 2.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286075, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (302.90, 142.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685154 reads in 89.160 CPU sec, 3.046 real sec
[M::process] read 684872 sequences (100000036 bp) in 2.281 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285825, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 285, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (304.60, 142.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1086605 mem = 536883468 peak = 978415616
[M::mem_process_seqs] Processed 684984 reads in 86.150 CPU sec, 3.014 real sec
[M::process] read 684588 sequences (100000216 bp) in 2.004 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285522, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (303.89, 142.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684872 reads in 89.783 CPU sec, 3.222 real sec
sorting block 50, #reads = 1088127 mem = 536878711 peak = 978415616
[M::process] read 684554 sequences (100000232 bp) in 2.414 CPU sec, 2.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285554, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (304.04, 141.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684588 reads in 84.602 CPU sec, 2.971 real sec
[M::process] read 684504 sequences (100000201 bp) in 2.299 CPU sec, 2.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286189, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (304.42, 141.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684554 reads in 86.879 CPU sec, 2.997 real sec
sorting block 51, #reads = 1087446 mem = 536878260 peak = 978415616
[M::process] read 684218 sequences (100000043 bp) in 2.480 CPU sec, 2.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285420, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (305.80, 143.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684504 reads in 90.372 CPU sec, 3.110 real sec
[M::process] read 682798 sequences (100000051 bp) in 2.149 CPU sec, 2.405 real sec...
sorting block 52, #reads = 1087840 mem = 536877663 peak = 978415616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285770, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (307.34, 144.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684218 reads in 86.721 CPU sec, 3.053 real sec
[M::process] read 681936 sequences (100000288 bp) in 2.248 CPU sec, 2.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284835, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 295, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (312.65, 144.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1087197 mem = 536879549 peak = 978415616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285189, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 300, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (316.85, 144.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683634 sequences (100000209 bp) in 2.582 CPU sec, 2.822 real sec...
[M::mem_process_seqs] Processed 682798 reads in 160.040 CPU sec, 5.234 real sec
[M::mem_process_seqs] Processed 681936 reads in 102.482 CPU sec, 3.388 real sec
[M::process] read 684162 sequences (100000002 bp) in 2.318 CPU sec, 2.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285383, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 289, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (308.15, 143.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1085918 mem = 536879027 peak = 978415616
[M::mem_process_seqs] Processed 683634 reads in 86.408 CPU sec, 3.023 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286190, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (304.92, 142.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684118 sequences (100000073 bp) in 2.284 CPU sec, 2.535 real sec...
[M::mem_process_seqs] Processed 684162 reads in 86.112 CPU sec, 2.924 real sec
sorting block 55, #reads = 1086215 mem = 536880287 peak = 978415616
[M::process] read 683896 sequences (100000047 bp) in 2.438 CPU sec, 2.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285187, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (305.44, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684118 reads in 86.937 CPU sec, 2.945 real sec
[M::process] read 684064 sequences (100000108 bp) in 2.248 CPU sec, 2.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285346, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (304.80, 142.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1087510 mem = 536880627 peak = 979709952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285511, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (305.13, 143.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684302 sequences (100000299 bp) in 2.446 CPU sec, 2.649 real sec...
[M::mem_process_seqs] Processed 683896 reads in 159.442 CPU sec, 5.226 real sec
[M::mem_process_seqs] Processed 684064 reads in 102.643 CPU sec, 3.349 real sec
sorting block 57, #reads = 1086718 mem = 536882166 peak = 979709952
[M::process] read 684308 sequences (100000234 bp) in 2.317 CPU sec, 2.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285071, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.83, 141.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285282, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 280, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (300.44, 139.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684364 sequences (100000106 bp) in 2.471 CPU sec, 2.912 real sec...
[M::mem_process_seqs] Processed 684302 reads in 160.027 CPU sec, 5.239 real sec
[M::mem_process_seqs] Processed 684308 reads in 106.765 CPU sec, 3.462 real sec
sorting block 58, #reads = 1086160 mem = 536882074 peak = 979709952
[M::process] read 684544 sequences (100000163 bp) in 2.423 CPU sec, 2.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285262, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.55, 142.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684364 reads in 85.349 CPU sec, 2.923 real sec
[M::process] read 684452 sequences (100000232 bp) in 2.345 CPU sec, 2.654 real sec...
sorting block 59, #reads = 1087085 mem = 536878494 peak = 979709952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285513, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (301.60, 141.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684544 reads in 89.812 CPU sec, 3.045 real sec
[M::process] read 684342 sequences (100000263 bp) in 2.248 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285720, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (301.37, 141.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1087625 mem = 536879838 peak = 979709952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285226, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.30, 141.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684298 sequences (100000118 bp) in 2.184 CPU sec, 2.502 real sec...
[M::mem_process_seqs] Processed 684452 reads in 154.479 CPU sec, 5.106 real sec
[M::mem_process_seqs] Processed 684342 reads in 102.101 CPU sec, 3.414 real sec
[M::process] read 684144 sequences (100000221 bp) in 2.432 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286293, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.71, 141.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1087063 mem = 536878673 peak = 979709952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285579, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (303.73, 142.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684382 sequences (100000153 bp) in 2.186 CPU sec, 2.527 real sec...
[M::mem_process_seqs] Processed 684298 reads in 157.907 CPU sec, 5.158 real sec
[M::mem_process_seqs] Processed 684144 reads in 100.256 CPU sec, 3.283 real sec
sorting block 62, #reads = 1087599 mem = 536880736 peak = 979709952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285651, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (302.96, 142.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684442 sequences (100000057 bp) in 2.596 CPU sec, 2.925 real sec...
[M::mem_process_seqs] Processed 684382 reads in 90.619 CPU sec, 3.047 real sec
[M::process] read 684494 sequences (100000076 bp) in 2.445 CPU sec, 2.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285767, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (303.22, 143.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1087786 mem = 536878099 peak = 979709952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285812, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.34, 143.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684884 sequences (100000275 bp) in 2.261 CPU sec, 2.579 real sec...
[M::mem_process_seqs] Processed 684442 reads in 160.972 CPU sec, 5.259 real sec
[M::mem_process_seqs] Processed 684494 reads in 107.305 CPU sec, 3.495 real sec
sorting block 64, #reads = 1087066 mem = 536879078 peak = 983515136
[M::process] read 683530 sequences (100000016 bp) in 2.241 CPU sec, 2.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285854, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (301.08, 140.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285085, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 288, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (304.49, 138.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682892 sequences (100000267 bp) in 2.403 CPU sec, 2.653 real sec...
[M::mem_process_seqs] Processed 684884 reads in 157.666 CPU sec, 5.130 real sec
[M::mem_process_seqs] Processed 683530 reads in 103.370 CPU sec, 3.334 real sec
sorting block 65, #reads = 1085497 mem = 536880403 peak = 983515136
[M::process] read 683072 sequences (100000237 bp) in 2.472 CPU sec, 2.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284767, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 293, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (309.88, 141.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284724, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 292, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (307.83, 140.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683226 sequences (100000226 bp) in 2.411 CPU sec, 2.599 real sec...
sorting block 66, #reads = 1086441 mem = 536877143 peak = 983515136
[M::mem_process_seqs] Processed 682892 reads in 155.663 CPU sec, 5.063 real sec
[M::mem_process_seqs] Processed 683072 reads in 101.625 CPU sec, 3.277 real sec
[M::process] read 684182 sequences (100000014 bp) in 2.405 CPU sec, 2.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284725, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 290, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (307.55, 141.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683226 reads in 85.603 CPU sec, 2.925 real sec
sorting block 67, #reads = 1086447 mem = 536878362 peak = 983515136
[M::process] read 683722 sequences (100000033 bp) in 2.187 CPU sec, 2.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285252, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (302.29, 138.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285267, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 287, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (303.54, 138.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683442 sequences (100000243 bp) in 2.335 CPU sec, 2.687 real sec...
[M::mem_process_seqs] Processed 684182 reads in 151.994 CPU sec, 5.002 real sec
[M::mem_process_seqs] Processed 683722 reads in 99.320 CPU sec, 3.271 real sec
sorting block 68, #reads = 1086197 mem = 536878445 peak = 983515136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284695, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 291, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (308.07, 141.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (721, 4565, 4922)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13324)
[M::mem_pestat] mean and std.dev: (2785.80, 2067.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17525)
[M::mem_pestat] skip orientation RR
[M::process] read 681508 sequences (100000059 bp) in 2.312 CPU sec, 2.596 real sec...
[M::mem_process_seqs] Processed 683442 reads in 83.816 CPU sec, 2.838 real sec
sorting block 69, #reads = 1087389 mem = 536877908 peak = 983515136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284163, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (318.09, 144.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682590 sequences (100000251 bp) in 2.422 CPU sec, 2.728 real sec...
[M::mem_process_seqs] Processed 681508 reads in 81.041 CPU sec, 2.800 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285053, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (314.42, 144.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685530 sequences (100000071 bp) in 2.338 CPU sec, 2.562 real sec...
[M::mem_process_seqs] Processed 682590 reads in 83.515 CPU sec, 2.843 real sec
sorting block 70, #reads = 1086174 mem = 536879134 peak = 983515136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285968, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (302.54, 142.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684766 sequences (100000025 bp) in 2.398 CPU sec, 2.732 real sec...
[M::mem_process_seqs] Processed 685530 reads in 82.719 CPU sec, 2.843 real sec
sorting block 71, #reads = 1086537 mem = 536883874 peak = 983515136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285635, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (305.72, 143.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685224 sequences (100000073 bp) in 2.470 CPU sec, 2.642 real sec...
[M::mem_process_seqs] Processed 684766 reads in 88.370 CPU sec, 2.968 real sec
[M::process] read 684882 sequences (100000153 bp) in 2.454 CPU sec, 2.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285933, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (302.00, 141.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685224 reads in 87.472 CPU sec, 2.960 real sec
sorting block 72, #reads = 1087326 mem = 536877716 peak = 983515136
[M::process] read 685260 sequences (100000018 bp) in 2.192 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285700, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.23, 140.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286041, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (301.81, 141.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685476 sequences (100000211 bp) in 2.538 CPU sec, 2.846 real sec...
[M::mem_process_seqs] Processed 684882 reads in 158.121 CPU sec, 5.168 real sec
sorting block 73, #reads = 1087553 mem = 536878277 peak = 983515136
[M::mem_process_seqs] Processed 685260 reads in 104.717 CPU sec, 3.400 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285979, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (302.75, 142.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684844 sequences (100000250 bp) in 2.322 CPU sec, 2.601 real sec...
[M::mem_process_seqs] Processed 685476 reads in 85.979 CPU sec, 2.963 real sec
sorting block 74, #reads = 1087734 mem = 536878323 peak = 983515136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286180, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (303.37, 142.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684758 sequences (100000063 bp) in 2.659 CPU sec, 2.980 real sec...
[M::mem_process_seqs] Processed 684844 reads in 88.122 CPU sec, 2.995 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286108, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (303.54, 142.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682376 sequences (100000299 bp) in 2.271 CPU sec, 2.582 real sec...
[M::mem_process_seqs] Processed 684758 reads in 87.343 CPU sec, 3.009 real sec
sorting block 75, #reads = 1088135 mem = 536878514 peak = 983515136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284715, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (315.36, 145.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682958 sequences (100000028 bp) in 2.635 CPU sec, 2.832 real sec...
[M::mem_process_seqs] Processed 682376 reads in 86.806 CPU sec, 2.967 real sec
sorting block 76, #reads = 1087836 mem = 536883921 peak = 988409856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284762, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 294, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (311.63, 144.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684290 sequences (100000289 bp) in 2.436 CPU sec, 2.885 real sec...
[M::mem_process_seqs] Processed 682958 reads in 86.610 CPU sec, 2.972 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285575, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (305.92, 143.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684566 sequences (100000175 bp) in 2.591 CPU sec, 2.788 real sec...
[M::mem_process_seqs] Processed 684290 reads in 88.529 CPU sec, 2.970 real sec
sorting block 77, #reads = 1086757 mem = 536880441 peak = 988409856
[M::process] read 683846 sequences (100000018 bp) in 2.277 CPU sec, 2.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285670, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (304.10, 142.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285502, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 289, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (308.27, 144.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1086619 mem = 536877762 peak = 988409856
[M::process] read 683052 sequences (100000069 bp) in 2.480 CPU sec, 2.677 real sec...
[M::mem_process_seqs] Processed 684566 reads in 157.892 CPU sec, 5.139 real sec
[M::mem_process_seqs] Processed 683846 reads in 104.744 CPU sec, 3.373 real sec
[M::process] read 684122 sequences (100000195 bp) in 2.546 CPU sec, 2.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284780, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (312.95, 146.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 79, #reads = 1087034 mem = 536877592 peak = 988409856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285760, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (305.74, 143.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684082 sequences (100000050 bp) in 2.383 CPU sec, 2.618 real sec...
[M::mem_process_seqs] Processed 683052 reads in 157.803 CPU sec, 5.270 real sec
[M::mem_process_seqs] Processed 684122 reads in 105.277 CPU sec, 3.606 real sec
[M::process] read 684094 sequences (100000179 bp) in 2.339 CPU sec, 2.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285003, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (304.76, 142.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1087148 mem = 536882163 peak = 988409856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285515, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (302.05, 140.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684448 sequences (100000299 bp) in 2.072 CPU sec, 2.624 real sec...
[M::mem_process_seqs] Processed 684082 reads in 162.323 CPU sec, 5.365 real sec
[M::mem_process_seqs] Processed 684094 reads in 103.275 CPU sec, 3.440 real sec
sorting block 81, #reads = 1086114 mem = 536879187 peak = 988409856
[M::process] read 684478 sequences (100000082 bp) in 2.467 CPU sec, 2.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285667, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (300.49, 140.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684448 reads in 93.891 CPU sec, 3.153 real sec
[M::process] read 684830 sequences (100000274 bp) in 2.432 CPU sec, 2.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285413, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (300.73, 140.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 82, #reads = 1086593 mem = 536879096 peak = 988409856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285367, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (300.90, 140.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684414 sequences (100000274 bp) in 2.107 CPU sec, 2.651 real sec...
[M::mem_process_seqs] Processed 684478 reads in 163.657 CPU sec, 5.501 real sec
[M::mem_process_seqs] Processed 684830 reads in 108.479 CPU sec, 3.653 real sec
sorting block 83, #reads = 1085884 mem = 536889298 peak = 988409856
[M::process] read 684278 sequences (100000183 bp) in 2.381 CPU sec, 2.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286151, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (300.22, 140.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684414 reads in 88.370 CPU sec, 3.026 real sec
[M::process] read 684452 sequences (100000195 bp) in 2.264 CPU sec, 2.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285699, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (302.83, 142.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 84, #reads = 1085055 mem = 536880129 peak = 988409856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285862, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (302.96, 142.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684088 sequences (100000210 bp) in 2.373 CPU sec, 2.806 real sec...
[M::mem_process_seqs] Processed 684278 reads in 163.885 CPU sec, 5.468 real sec
[M::mem_process_seqs] Processed 684452 reads in 104.809 CPU sec, 3.447 real sec
sorting block 85, #reads = 1085894 mem = 536887012 peak = 988409856
[M::process] read 685084 sequences (100000140 bp) in 2.234 CPU sec, 2.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285550, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.73, 141.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285360, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (299.57, 141.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688966 sequences (100000223 bp) in 2.395 CPU sec, 2.925 real sec...
[M::mem_process_seqs] Processed 684088 reads in 164.182 CPU sec, 5.375 real sec
[M::mem_process_seqs] Processed 685084 reads in 106.517 CPU sec, 3.492 real sec
sorting block 86, #reads = 1086936 mem = 536877974 peak = 988409856
[M::process] read 687606 sequences (100000194 bp) in 2.435 CPU sec, 2.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287808, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 262, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (287.77, 140.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686642 sequences (100000295 bp) in 2.454 CPU sec, 2.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287105, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 273, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (300.27, 148.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688966 reads in 177.347 CPU sec, 5.781 real sec
[M::mem_process_seqs] Processed 687606 reads in 114.841 CPU sec, 3.723 real sec
sorting block 87, #reads = 1086559 mem = 536878516 peak = 988409856
[M::process] read 687238 sequences (100000298 bp) in 2.308 CPU sec, 2.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286736, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 277, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (303.34, 148.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 88, #reads = 1085039 mem = 536885552 peak = 988409856
[M::process] read 688210 sequences (100000059 bp) in 2.342 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287741, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 273, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (299.37, 146.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686642 reads in 170.981 CPU sec, 5.586 real sec
[M::mem_process_seqs] Processed 687238 reads in 111.743 CPU sec, 3.645 real sec
[M::process] read 688080 sequences (100000056 bp) in 2.371 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287288, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (292.52, 142.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 89, #reads = 1084936 mem = 536880405 peak = 988409856
[M::process] read 688368 sequences (100000013 bp) in 2.366 CPU sec, 2.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287277, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (294.42, 143.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688210 reads in 169.278 CPU sec, 5.557 real sec
[M::mem_process_seqs] Processed 688080 reads in 112.726 CPU sec, 3.687 real sec
[M::process] read 687912 sequences (100000270 bp) in 2.122 CPU sec, 2.337 real sec...
sorting block 90, #reads = 1086960 mem = 536879840 peak = 988409856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287458, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (292.02, 142.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684794 sequences (100000214 bp) in 2.389 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287221, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (294.03, 142.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688368 reads in 170.592 CPU sec, 5.587 real sec
[M::mem_process_seqs] Processed 687912 reads in 113.435 CPU sec, 3.668 real sec
sorting block 91, #reads = 1085574 mem = 536881024 peak = 988409856
[M::process] read 687118 sequences (100000295 bp) in 2.321 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 286539, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (518, 596, 776)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 1292)
[M::mem_pestat] mean and std.dev: (605.80, 163.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1550)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (312.66, 151.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688266 sequences (100000197 bp) in 2.481 CPU sec, 2.843 real sec...
sorting block 92, #reads = 1086985 mem = 536878850 peak = 988409856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286858, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 274, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (298.50, 144.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684794 reads in 169.958 CPU sec, 5.539 real sec
[M::mem_process_seqs] Processed 687118 reads in 112.506 CPU sec, 3.632 real sec
[M::process] read 688750 sequences (100000250 bp) in 2.402 CPU sec, 2.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287213, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (292.83, 142.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 93, #reads = 1084320 mem = 536878571 peak = 988409856
[M::process] read 687574 sequences (100000279 bp) in 2.399 CPU sec, 2.810 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287083, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (289.86, 140.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688266 reads in 174.569 CPU sec, 5.655 real sec
[M::mem_process_seqs] Processed 688750 reads in 115.578 CPU sec, 3.740 real sec
[M::process] read 686914 sequences (100000078 bp) in 2.222 CPU sec, 2.372 real sec...
sorting block 94, #reads = 1085965 mem = 536877880 peak = 988409856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286857, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (297.38, 145.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685324 sequences (100000205 bp) in 2.208 CPU sec, 2.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286499, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (299.76, 145.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687574 reads in 173.238 CPU sec, 5.612 real sec
[M::mem_process_seqs] Processed 686914 reads in 113.241 CPU sec, 3.658 real sec
[M::process] read 683980 sequences (100000045 bp) in 1.978 CPU sec, 2.132 real sec...
sorting block 95, #reads = 1084938 mem = 536883290 peak = 988409856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286176, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 282, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (305.29, 146.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685834 sequences (100000106 bp) in 2.550 CPU sec, 2.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285362, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (312.72, 149.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (439, 439, 997)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2113)
[M::mem_pestat] mean and std.dev: (622.67, 332.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2671)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685324 reads in 172.425 CPU sec, 5.582 real sec
[M::mem_process_seqs] Processed 683980 reads in 113.217 CPU sec, 3.630 real sec
sorting block 96, #reads = 1084292 mem = 536882642 peak = 988409856
[M::process] read 686146 sequences (100000179 bp) in 2.171 CPU sec, 2.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286353, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (302.83, 146.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 97, #reads = 1083748 mem = 536877344 peak = 999510016
[M::process] read 683672 sequences (100000091 bp) in 2.369 CPU sec, 2.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286796, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.35, 144.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685834 reads in 173.610 CPU sec, 5.693 real sec
[M::mem_process_seqs] Processed 686146 reads in 113.885 CPU sec, 3.795 real sec
[M::process] read 685324 sequences (100000194 bp) in 2.050 CPU sec, 2.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285760, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (310.62, 146.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 98, #reads = 1084921 mem = 536879156 peak = 999510016
[M::process] read 684494 sequences (100000179 bp) in 2.480 CPU sec, 3.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286074, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (300.07, 141.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683672 reads in 173.021 CPU sec, 5.630 real sec
[M::mem_process_seqs] Processed 685324 reads in 113.587 CPU sec, 3.656 real sec
[M::process] read 685052 sequences (100000023 bp) in 2.301 CPU sec, 2.425 real sec...
sorting block 99, #reads = 1083430 mem = 536881221 peak = 999510016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285977, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (304.33, 143.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683238 sequences (100000218 bp) in 2.405 CPU sec, 2.774 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286068, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (301.84, 142.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684494 reads in 172.692 CPU sec, 5.606 real sec
[M::mem_process_seqs] Processed 685052 reads in 113.690 CPU sec, 3.633 real sec
[M::process] read 684256 sequences (100000066 bp) in 2.193 CPU sec, 2.310 real sec...
sorting block 100, #reads = 1083129 mem = 536880081 peak = 999510016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285590, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (312.67, 147.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685382 sequences (100000254 bp) in 2.466 CPU sec, 2.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285607, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 285, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (307.37, 145.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683238 reads in 172.800 CPU sec, 5.568 real sec
sorting block 101, #reads = 1084125 mem = 536877866 peak = 999510016
[M::mem_process_seqs] Processed 684256 reads in 114.177 CPU sec, 3.665 real sec
[M::process] read 685042 sequences (100000036 bp) in 2.239 CPU sec, 2.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286059, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (300.65, 143.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 102, #reads = 1083515 mem = 536880090 peak = 1027129344
[M::process] read 683350 sequences (100000122 bp) in 2.607 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286147, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (300.71, 142.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685382 reads in 176.261 CPU sec, 5.734 real sec
[M::mem_process_seqs] Processed 685042 reads in 115.391 CPU sec, 3.700 real sec
[M::process] read 685052 sequences (100000247 bp) in 2.075 CPU sec, 2.136 real sec...
sorting block 103, #reads = 1083659 mem = 536880148 peak = 1027129344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285471, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (310.05, 146.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685220 sequences (100000040 bp) in 2.479 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285853, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (302.68, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683350 reads in 173.850 CPU sec, 5.632 real sec
[M::mem_process_seqs] Processed 685052 reads in 113.656 CPU sec, 3.640 real sec
[M::process] read 685014 sequences (100000261 bp) in 1.952 CPU sec, 2.012 real sec...
sorting block 104, #reads = 1082895 mem = 536880181 peak = 1027129344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286329, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.37, 144.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684062 sequences (100000260 bp) in 2.295 CPU sec, 2.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286155, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (303.18, 145.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685220 reads in 171.615 CPU sec, 5.602 real sec
[M::mem_process_seqs] Processed 685014 reads in 113.232 CPU sec, 3.672 real sec
[M::process] read 690248 sequences (100000018 bp) in 2.138 CPU sec, 2.332 real sec...
sorting block 105, #reads = 1082856 mem = 536879549 peak = 1027129344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285713, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (309.27, 147.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691596 sequences (100000168 bp) in 2.250 CPU sec, 2.613 real sec...
sorting block 106, #reads = 1084425 mem = 536881651 peak = 1062412288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288098, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (285.96, 139.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684062 reads in 181.709 CPU sec, 5.875 real sec
[M::process] read 689422 sequences (100000035 bp) in 2.163 CPU sec, 2.303 real sec...
[M::mem_process_seqs] Processed 690248 reads in 127.008 CPU sec, 4.076 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289151, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (280.27, 135.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 107, #reads = 1082190 mem = 536879820 peak = 1062412288
[M::process] read 689898 sequences (100000059 bp) in 2.756 CPU sec, 3.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287875, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 267, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (289.77, 138.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689036 sequences (100000228 bp) in 1.899 CPU sec, 1.975 real sec...
[M::mem_process_seqs] Processed 691596 reads in 199.340 CPU sec, 6.454 real sec
[M::mem_process_seqs] Processed 689422 reads in 132.854 CPU sec, 4.291 real sec
sorting block 108, #reads = 1079811 mem = 536879091 peak = 1062412288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287580, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 262, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (286.23, 137.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688672 sequences (100000136 bp) in 2.882 CPU sec, 3.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288139, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (289.16, 138.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689114 sequences (100000030 bp) in 2.055 CPU sec, 2.072 real sec...
[M::mem_process_seqs] Processed 689898 reads in 192.245 CPU sec, 6.188 real sec
[M::mem_process_seqs] Processed 689036 reads in 125.969 CPU sec, 4.023 real sec
sorting block 109, #reads = 1079217 mem = 536883650 peak = 1062412288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287563, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (288.80, 137.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686940 sequences (100000046 bp) in 2.874 CPU sec, 3.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288349, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.22, 136.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687620 sequences (100000147 bp) in 1.930 CPU sec, 1.936 real sec...
[M::mem_process_seqs] Processed 688672 reads in 189.385 CPU sec, 6.087 real sec
sorting block 110, #reads = 1081788 mem = 536879053 peak = 1062412288
[M::mem_process_seqs] Processed 689114 reads in 124.517 CPU sec, 3.971 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286252, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (296.16, 140.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 111, #reads = 1082348 mem = 536880336 peak = 1073123328
[M::process] read 688408 sequences (100000110 bp) in 2.877 CPU sec, 3.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287514, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 272, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (294.83, 141.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686940 reads in 180.630 CPU sec, 5.882 real sec
[M::mem_process_seqs] Processed 687620 reads in 116.230 CPU sec, 3.790 real sec
[M::process] read 688808 sequences (100000045 bp) in 1.952 CPU sec, 2.308 real sec...
sorting block 112, #reads = 1082850 mem = 536877805 peak = 1073123328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287422, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (288.94, 137.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688504 sequences (100000060 bp) in 2.568 CPU sec, 3.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287748, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (288.95, 138.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688408 reads in 178.754 CPU sec, 5.783 real sec
[M::process] read 687630 sequences (100000093 bp) in 1.919 CPU sec, 1.964 real sec...
[M::mem_process_seqs] Processed 688808 reads in 116.607 CPU sec, 3.743 real sec
sorting block 113, #reads = 1083339 mem = 536876772 peak = 1073123328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288086, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (290.11, 139.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686194 sequences (100000149 bp) in 2.837 CPU sec, 3.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287489, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (293.04, 140.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688504 reads in 184.510 CPU sec, 5.948 real sec
[M::process] read 684272 sequences (100000043 bp) in 2.137 CPU sec, 2.315 real sec...
[M::mem_process_seqs] Processed 687630 reads in 121.553 CPU sec, 3.882 real sec
sorting block 114, #reads = 1084050 mem = 536876868 peak = 1073123328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286488, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (301.44, 144.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686756 sequences (100000047 bp) in 2.767 CPU sec, 3.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285762, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (312.25, 148.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686194 reads in 178.819 CPU sec, 5.751 real sec
sorting block 115, #reads = 1084065 mem = 536886267 peak = 1073123328
[M::process] read 686132 sequences (100000227 bp) in 2.040 CPU sec, 2.074 real sec...
[M::mem_process_seqs] Processed 684272 reads in 116.466 CPU sec, 3.724 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286752, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (299.36, 144.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 116, #reads = 1083421 mem = 536878342 peak = 1085845504
[M::process] read 685298 sequences (100000191 bp) in 2.912 CPU sec, 3.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286612, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (301.93, 144.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686756 reads in 179.003 CPU sec, 5.817 real sec
[M::mem_process_seqs] Processed 686132 reads in 118.356 CPU sec, 3.821 real sec
[M::process] read 685306 sequences (100000068 bp) in 2.223 CPU sec, 2.308 real sec...
sorting block 117, #reads = 1082870 mem = 536882287 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286263, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 281, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (304.15, 145.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685048 sequences (100000300 bp) in 2.808 CPU sec, 3.255 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286419, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (302.72, 144.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685298 reads in 177.037 CPU sec, 5.776 real sec
[M::mem_process_seqs] Processed 685306 reads in 114.877 CPU sec, 3.750 real sec
[M::process] read 684886 sequences (100000239 bp) in 1.974 CPU sec, 2.134 real sec...
sorting block 118, #reads = 1083539 mem = 536877622 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286993, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (305.41, 146.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683204 sequences (100000299 bp) in 2.834 CPU sec, 3.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286101, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.58, 145.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685048 reads in 175.452 CPU sec, 5.653 real sec
[M::mem_process_seqs] Processed 684886 reads in 113.917 CPU sec, 3.605 real sec
sorting block 119, #reads = 1083428 mem = 536879090 peak = 1085845504
[M::process] read 684548 sequences (100000294 bp) in 2.272 CPU sec, 2.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285536, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (314.77, 149.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 120, #reads = 1083972 mem = 536879593 peak = 1085845504
[M::process] read 685692 sequences (100000064 bp) in 2.480 CPU sec, 2.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286466, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (307.12, 147.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683204 reads in 172.085 CPU sec, 5.578 real sec
[M::process] read 685144 sequences (100000076 bp) in 1.921 CPU sec, 1.962 real sec...
[M::mem_process_seqs] Processed 684548 reads in 113.460 CPU sec, 3.629 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286596, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.92, 144.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 121, #reads = 1082795 mem = 536878315 peak = 1085845504
[M::process] read 685196 sequences (100000301 bp) in 2.857 CPU sec, 3.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286574, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.53, 144.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685692 reads in 170.683 CPU sec, 5.538 real sec
[M::mem_process_seqs] Processed 685144 reads in 111.615 CPU sec, 3.593 real sec
[M::process] read 685520 sequences (100000285 bp) in 2.168 CPU sec, 2.284 real sec...
sorting block 122, #reads = 1085680 mem = 536879926 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285962, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (303.91, 146.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685546 sequences (100000162 bp) in 2.888 CPU sec, 3.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286590, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (303.72, 147.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685196 reads in 174.475 CPU sec, 5.636 real sec
[M::mem_process_seqs] Processed 685520 reads in 112.475 CPU sec, 3.594 real sec
[M::process] read 685724 sequences (100000223 bp) in 1.866 CPU sec, 1.941 real sec...
sorting block 123, #reads = 1084468 mem = 536876940 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286116, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (302.80, 147.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687418 sequences (100000086 bp) in 3.013 CPU sec, 3.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285425, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (300.60, 145.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685546 reads in 184.964 CPU sec, 5.988 real sec
[M::process] read 688954 sequences (100000277 bp) in 2.010 CPU sec, 2.065 real sec...
[M::mem_process_seqs] Processed 685724 reads in 121.431 CPU sec, 3.875 real sec
sorting block 124, #reads = 1084251 mem = 536878203 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286786, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (294.40, 143.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 125, #reads = 1081345 mem = 536878811 peak = 1085845504
[M::process] read 688362 sequences (100000158 bp) in 3.010 CPU sec, 3.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287660, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 267, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (289.74, 138.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687418 reads in 196.864 CPU sec, 6.390 real sec
[M::process] read 687852 sequences (100000255 bp) in 1.727 CPU sec, 1.762 real sec...
[M::mem_process_seqs] Processed 688954 reads in 117.858 CPU sec, 3.769 real sec
sorting block 126, #reads = 1076378 mem = 536878452 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287527, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 270, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (293.70, 141.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687208 sequences (100000212 bp) in 2.768 CPU sec, 3.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287153, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 271, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (294.32, 141.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688362 reads in 166.087 CPU sec, 5.406 real sec
[M::mem_process_seqs] Processed 687852 reads in 107.368 CPU sec, 3.480 real sec
[M::process] read 687110 sequences (100000295 bp) in 2.588 CPU sec, 3.080 real sec...
sorting block 127, #reads = 1086366 mem = 536882735 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286870, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (296.77, 141.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686738 sequences (100000143 bp) in 2.572 CPU sec, 3.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286763, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (297.85, 142.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687208 reads in 166.480 CPU sec, 5.396 real sec
[M::mem_process_seqs] Processed 687110 reads in 110.619 CPU sec, 3.575 real sec
sorting block 128, #reads = 1087138 mem = 536881824 peak = 1085845504
[M::process] read 683578 sequences (100000252 bp) in 2.263 CPU sec, 2.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286606, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (299.69, 143.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685782 sequences (100000101 bp) in 2.579 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285469, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (310.50, 143.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686738 reads in 166.352 CPU sec, 5.439 real sec
sorting block 129, #reads = 1086564 mem = 536880714 peak = 1085845504
[M::mem_process_seqs] Processed 683578 reads in 108.237 CPU sec, 3.554 real sec
[M::process] read 687810 sequences (100000210 bp) in 2.303 CPU sec, 2.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286506, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (303.15, 143.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 130, #reads = 1085740 mem = 536878834 peak = 1085845504
[M::process] read 687740 sequences (100000175 bp) in 2.936 CPU sec, 3.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286904, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (291.03, 138.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685782 reads in 170.797 CPU sec, 5.505 real sec
[M::mem_process_seqs] Processed 687810 reads in 114.650 CPU sec, 3.647 real sec
[M::process] read 688318 sequences (100000231 bp) in 2.229 CPU sec, 2.505 real sec...
sorting block 131, #reads = 1085540 mem = 536879188 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287176, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (290.41, 136.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688090 sequences (100000002 bp) in 2.596 CPU sec, 2.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287531, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (289.35, 137.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687740 reads in 172.715 CPU sec, 5.575 real sec
[M::mem_process_seqs] Processed 688318 reads in 115.684 CPU sec, 3.691 real sec
[M::process] read 687100 sequences (100000103 bp) in 2.188 CPU sec, 2.253 real sec...
sorting block 132, #reads = 1086523 mem = 536886544 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287163, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (289.94, 137.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687068 sequences (100000140 bp) in 2.612 CPU sec, 3.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287021, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (292.89, 138.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688090 reads in 170.929 CPU sec, 5.595 real sec
[M::mem_process_seqs] Processed 687100 reads in 109.048 CPU sec, 3.524 real sec
sorting block 133, #reads = 1085818 mem = 536880014 peak = 1085845504
[M::process] read 687214 sequences (100000162 bp) in 2.215 CPU sec, 2.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286635, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (294.62, 139.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 134, #reads = 1085156 mem = 536880743 peak = 1085845504
[M::process] read 685388 sequences (100000170 bp) in 2.714 CPU sec, 2.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287020, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (295.60, 141.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687068 reads in 168.274 CPU sec, 5.404 real sec
[M::mem_process_seqs] Processed 687214 reads in 110.954 CPU sec, 3.545 real sec
[M::process] read 684560 sequences (100000036 bp) in 1.987 CPU sec, 2.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285981, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.90, 144.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (321, 321, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1728)
[M::mem_pestat] mean and std.dev: (484.92, 247.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2197)
[M::mem_pestat] skip orientation RR
sorting block 135, #reads = 1087184 mem = 536880633 peak = 1085845504
[M::process] read 686654 sequences (100000143 bp) in 2.788 CPU sec, 3.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286271, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (306.86, 144.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685388 reads in 167.948 CPU sec, 5.432 real sec
[M::mem_process_seqs] Processed 684560 reads in 110.472 CPU sec, 3.532 real sec
[M::process] read 686334 sequences (100000020 bp) in 2.212 CPU sec, 2.349 real sec...
sorting block 136, #reads = 1084791 mem = 536877758 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286740, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (295.36, 139.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686550 sequences (100000160 bp) in 2.578 CPU sec, 2.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286478, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (297.29, 140.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686654 reads in 166.326 CPU sec, 5.348 real sec
[M::mem_process_seqs] Processed 686334 reads in 106.694 CPU sec, 3.425 real sec
[M::process] read 686410 sequences (100000293 bp) in 1.905 CPU sec, 2.013 real sec...
sorting block 137, #reads = 1085281 mem = 536877191 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286821, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (297.30, 140.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685936 sequences (100000072 bp) in 3.068 CPU sec, 3.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286592, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (298.74, 142.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686550 reads in 170.416 CPU sec, 5.534 real sec
[M::mem_process_seqs] Processed 686410 reads in 110.048 CPU sec, 3.531 real sec
sorting block 138, #reads = 1086709 mem = 536882363 peak = 1085845504
[M::process] read 685742 sequences (100000077 bp) in 2.231 CPU sec, 2.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286939, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (300.40, 142.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 139, #reads = 1086088 mem = 536878176 peak = 1085845504
[M::process] read 685798 sequences (100000080 bp) in 2.663 CPU sec, 2.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286766, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (302.25, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685936 reads in 173.483 CPU sec, 5.596 real sec
[M::mem_process_seqs] Processed 685742 reads in 114.698 CPU sec, 3.697 real sec
[M::process] read 686058 sequences (100000145 bp) in 1.887 CPU sec, 1.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286690, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (301.51, 144.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 468, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382)
[M::mem_pestat] mean and std.dev: (299.15, 261.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1839)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 140, #reads = 1084351 mem = 536882301 peak = 1085845504
[M::process] read 686236 sequences (100000181 bp) in 3.163 CPU sec, 3.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286360, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.73, 144.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685798 reads in 181.740 CPU sec, 5.859 real sec
[M::mem_process_seqs] Processed 686058 reads in 124.325 CPU sec, 3.938 real sec
[M::process] read 686056 sequences (100000095 bp) in 2.335 CPU sec, 2.416 real sec...
sorting block 141, #reads = 1084154 mem = 536880166 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286608, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (300.32, 144.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685890 sequences (100000198 bp) in 2.436 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286814, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (301.79, 145.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686236 reads in 177.699 CPU sec, 5.742 real sec
[M::process] read 686664 sequences (100000282 bp) in 2.051 CPU sec, 2.116 real sec...
[M::mem_process_seqs] Processed 686056 reads in 119.052 CPU sec, 3.802 real sec
sorting block 142, #reads = 1084336 mem = 536881290 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286359, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (301.98, 145.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686552 sequences (100000005 bp) in 2.964 CPU sec, 3.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286931, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (296.17, 143.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685890 reads in 178.865 CPU sec, 5.815 real sec
sorting block 143, #reads = 1083361 mem = 536879090 peak = 1085845504
[M::mem_process_seqs] Processed 686664 reads in 119.565 CPU sec, 3.800 real sec
[M::process] read 686854 sequences (100000003 bp) in 2.288 CPU sec, 2.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287015, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 270, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (295.50, 142.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 144, #reads = 1083443 mem = 536879236 peak = 1085845504
[M::process] read 692272 sequences (100000019 bp) in 2.677 CPU sec, 2.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286322, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 270, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (294.88, 142.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689974 sequences (100000169 bp) in 1.982 CPU sec, 1.986 real sec...
[M::mem_process_seqs] Processed 686552 reads in 197.178 CPU sec, 6.327 real sec
[M::mem_process_seqs] Processed 686854 reads in 133.288 CPU sec, 4.257 real sec
sorting block 145, #reads = 1081607 mem = 536880175 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288865, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (280.06, 135.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689672 sequences (100000020 bp) in 2.829 CPU sec, 3.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287760, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 264, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (287.08, 137.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690578 sequences (100000074 bp) in 2.104 CPU sec, 2.110 real sec...
[M::mem_process_seqs] Processed 692272 reads in 203.862 CPU sec, 6.569 real sec
[M::mem_process_seqs] Processed 689974 reads in 133.354 CPU sec, 4.280 real sec
sorting block 146, #reads = 1076845 mem = 536880013 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287712, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 264, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (287.49, 137.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690020 sequences (100000152 bp) in 2.834 CPU sec, 3.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287915, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 259, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (282.21, 134.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686890 sequences (100000040 bp) in 2.112 CPU sec, 2.362 real sec...
[M::mem_process_seqs] Processed 689672 reads in 198.463 CPU sec, 6.348 real sec
sorting block 147, #reads = 1079629 mem = 536879256 peak = 1085845504
[M::mem_process_seqs] Processed 690578 reads in 129.714 CPU sec, 4.129 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287995, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 261, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (283.76, 134.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 148, #reads = 1079618 mem = 536879136 peak = 1085845504
[M::process] read 688954 sequences (100000076 bp) in 3.005 CPU sec, 3.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286794, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (302.59, 143.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689314 sequences (100000124 bp) in 1.913 CPU sec, 1.915 real sec...
[M::mem_process_seqs] Processed 690020 reads in 188.925 CPU sec, 6.026 real sec
[M::mem_process_seqs] Processed 686890 reads in 120.620 CPU sec, 3.817 real sec
sorting block 149, #reads = 1080356 mem = 536875545 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287369, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 267, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (289.64, 138.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690332 sequences (100000153 bp) in 2.905 CPU sec, 3.032 real sec...
[M::process] read 690692 sequences (100000087 bp) in 1.506 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287893, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 264, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (288.69, 139.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688954 reads in 178.151 CPU sec, 5.688 real sec
[M::mem_process_seqs] Processed 689314 reads in 114.904 CPU sec, 3.673 real sec
sorting block 150, #reads = 1081739 mem = 536880372 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288128, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 258, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (283.04, 136.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689366 sequences (100000066 bp) in 3.043 CPU sec, 3.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287869, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 258, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.70, 133.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690332 reads in 193.644 CPU sec, 6.215 real sec
[M::process] read 688846 sequences (100000004 bp) in 2.015 CPU sec, 2.203 real sec...
[M::mem_process_seqs] Processed 690692 reads in 127.996 CPU sec, 4.075 real sec
sorting block 151, #reads = 1083741 mem = 536882550 peak = 1085845504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288070, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 262, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (282.36, 132.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688122 sequences (100000260 bp) in 3.138 CPU sec, 3.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287373, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (286.24, 134.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689366 reads in 188.793 CPU sec, 6.046 real sec
sorting block 152, #reads = 1082924 mem = 536876083 peak = 1085845504
[M::process] read 685816 sequences (100000052 bp) in 2.152 CPU sec, 2.286 real sec...
[M::mem_process_seqs] Processed 688846 reads in 120.501 CPU sec, 3.815 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287446, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 267, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (289.53, 136.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 153, #reads = 1083635 mem = 536878509 peak = 1092583424
[M::process] read 686712 sequences (100000019 bp) in 3.371 CPU sec, 3.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286421, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (299.85, 139.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688122 reads in 186.521 CPU sec, 6.010 real sec
[M::mem_process_seqs] Processed 685816 reads in 120.576 CPU sec, 3.859 real sec
[M::process] read 687498 sequences (100000206 bp) in 2.343 CPU sec, 2.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286731, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (294.58, 137.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 154, #reads = 1084509 mem = 536881461 peak = 1092583424
[M::process] read 687308 sequences (100000289 bp) in 2.987 CPU sec, 3.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287024, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (292.42, 138.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686712 reads in 182.749 CPU sec, 5.886 real sec
[M::mem_process_seqs] Processed 687498 reads in 118.588 CPU sec, 3.778 real sec
[M::process] read 686412 sequences (100000171 bp) in 2.474 CPU sec, 2.671 real sec...
sorting block 155, #reads = 1082394 mem = 536879608 peak = 1092583424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287200, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (291.06, 136.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687234 sequences (100000156 bp) in 2.383 CPU sec, 2.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286632, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (297.86, 140.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687308 reads in 179.556 CPU sec, 5.813 real sec
[M::mem_process_seqs] Processed 686412 reads in 117.113 CPU sec, 3.757 real sec
[M::process] read 686888 sequences (100000096 bp) in 2.167 CPU sec, 2.238 real sec...
sorting block 156, #reads = 1084595 mem = 536877641 peak = 1092583424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287417, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (334, 414, 1978)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5266)
[M::mem_pestat] mean and std.dev: (1045.55, 815.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6910)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (293.38, 138.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686522 sequences (100000161 bp) in 2.993 CPU sec, 3.220 real sec...
sorting block 157, #reads = 1084239 mem = 536877912 peak = 1092583424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286367, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (293.26, 137.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687234 reads in 178.536 CPU sec, 5.718 real sec
[M::mem_process_seqs] Processed 686888 reads in 117.863 CPU sec, 3.740 real sec
[M::process] read 685942 sequences (100000220 bp) in 2.088 CPU sec, 2.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286658, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (298.42, 141.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 158, #reads = 1085143 mem = 536879453 peak = 1092583424
[M::process] read 686238 sequences (100000005 bp) in 2.903 CPU sec, 3.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286063, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (296.97, 139.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686522 reads in 179.866 CPU sec, 5.770 real sec
[M::process] read 686098 sequences (100000160 bp) in 1.893 CPU sec, 1.949 real sec...
[M::mem_process_seqs] Processed 685942 reads in 117.844 CPU sec, 3.743 real sec
sorting block 159, #reads = 1084734 mem = 536880447 peak = 1092583424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286487, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (296.51, 140.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685894 sequences (100000032 bp) in 2.816 CPU sec, 3.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286393, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (297.33, 140.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686238 reads in 179.313 CPU sec, 5.756 real sec
[M::mem_process_seqs] Processed 686098 reads in 120.220 CPU sec, 3.815 real sec
[M::process] read 685868 sequences (100000180 bp) in 2.823 CPU sec, 2.977 real sec...
sorting block 160, #reads = 1083090 mem = 536878967 peak = 1092583424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286247, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (298.32, 141.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685678 sequences (100000273 bp) in 2.960 CPU sec, 3.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286013, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (298.34, 142.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685894 reads in 178.893 CPU sec, 5.750 real sec
[M::mem_process_seqs] Processed 685868 reads in 118.179 CPU sec, 3.750 real sec
sorting block 161, #reads = 1084271 mem = 536881766 peak = 1092583424
[M::process] read 685492 sequences (100000208 bp) in 2.305 CPU sec, 2.400 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286182, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.18, 143.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 162, #reads = 1084873 mem = 536879901 peak = 1092583424
[M::process] read 527742 sequences (76787747 bp) in 2.379 CPU sec, 2.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285978, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (300.87, 143.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685678 reads in 163.363 CPU sec, 5.250 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 220443, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (293.20, 141.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 527742 reads in 71.788 CPU sec, 2.355 real sec
sorting block 163, #reads = 1084527 mem = 536879523 peak = 1092583424
[M::mem_process_seqs] Processed 685492 reads in 175.190 CPU sec, 6.056 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 178047414
overall: 69878992896 mem 25185.776 user 634.948 sys 868.944 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cfy-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:659_cfy-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cfy-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cfy-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cfy-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 868.988 sec; CPU: 25820.734 sec
sorting block 164, #reads = 923056 mem = 457342189 peak = 1140080640
uniq header count 1
merging 165 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 9341841424
output reads: 178934521
bam_mem_sort: 165 calls 117.897 user 3.463 sys 125.231 real
bam_write: 165 calls 97.146 user 7.038 sys 213.376 real
execute: 1 calls 55.240 user 2.426 sys 932.100 real
merge_files: 1 calls 55.228 user 2.392 sys 62.334 real
parse_chunk: 2638 calls 123.272 user 31.933 sys 210.874 real
read_chunk: 7112 calls 62.800 user 41.787 sys 821.763 real
sort_block: 1 calls 43.754 user 40.795 sys 794.280 real
write_chunk: 4472 calls 54.725 user 2.109 sys 61.151 real
overall: 3245432832 mem 2477.287 user 110.278 sys 932.119 real
ls: cannot access '*.cram': No such file or directory