cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_4XR-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1982.559 CPU sec, 77.273 real sec [M::process] read 682982 sequences (100000007 bp) in 0.964 CPU sec, 0.969 real sec... [M::process] read 682714 sequences (100000148 bp) in 1.110 CPU sec, 1.106 real sec... [M::process] read 681640 sequences (100000223 bp) in 1.128 CPU sec, 1.138 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 281237, 7, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 211, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (218.80, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 308, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (324.92, 153.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 255, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1398) [M::mem_pestat] mean and std.dev: (402.13, 286.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681348 sequences (100000294 bp) in 1.090 CPU sec, 1.091 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281050, 18, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 207, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (386.12, 315.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 311, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (327.65, 154.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (850, 1688, 1827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3781) [M::mem_pestat] mean and std.dev: (1383.00, 760.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 264, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (292.53, 136.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 280669, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 299, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313) [M::mem_pestat] mean and std.dev: (296.82, 150.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 317, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (332.91, 155.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (115, 2546, 9854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 29332) [M::mem_pestat] mean and std.dev: (3772.40, 4150.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 39071) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 368, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2463) [M::mem_pestat] mean and std.dev: (554.20, 512.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3201) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682982 reads in 217.355 CPU sec, 6.918 real sec [M::mem_process_seqs] Processed 682714 reads in 162.099 CPU sec, 5.209 real sec [M::mem_process_seqs] Processed 681640 reads in 178.409 CPU sec, 5.745 real sec sorting block 0, #reads = 1076136 mem = 536878620 peak = 599642112 [M::process] read 681202 sequences (100000297 bp) in 2.093 CPU sec, 2.233 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281060, 10, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (234.85, 100.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 321, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (336.58, 157.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 445, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283) [M::mem_pestat] mean and std.dev: (348.88, 196.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 382, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2956) [M::mem_pestat] mean and std.dev: (414.24, 306.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680918 sequences (100000055 bp) in 1.448 CPU sec, 1.458 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280413, 17, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 238, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (223.32, 81.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 321, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (336.30, 156.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 548, 4192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11992) [M::mem_pestat] mean and std.dev: (2161.29, 2351.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15892) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 249, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995) [M::mem_pestat] mean and std.dev: (322.07, 215.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680748 sequences (100000104 bp) in 1.300 CPU sec, 1.298 real sec... [M::mem_process_seqs] Processed 681348 reads in 152.431 CPU sec, 4.857 real sec sorting block 1, #reads = 1076434 mem = 536880691 peak = 819843072 [M::mem_process_seqs] Processed 681202 reads in 99.057 CPU sec, 3.159 real sec sorting block 2, #reads = 1077180 mem = 536880139 peak = 875806720 [M::process] read 680352 sequences (100000214 bp) in 1.724 CPU sec, 1.797 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280114, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 233, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (277.61, 149.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 322, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (337.96, 157.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 373, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1777) [M::mem_pestat] mean and std.dev: (315.53, 182.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680182 sequences (100000287 bp) in 1.399 CPU sec, 1.403 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280609, 18, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 240, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (210.32, 62.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 324, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (339.35, 157.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (362, 521, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2981) [M::mem_pestat] mean and std.dev: (651.71, 538.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 377, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (340.32, 179.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280764, 2, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 206, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (194.52, 73.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 327, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (341.01, 157.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 384, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (370.88, 162.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680918 reads in 221.297 CPU sec, 7.094 real sec [M::mem_process_seqs] Processed 680748 reads in 166.027 CPU sec, 5.347 real sec [M::mem_process_seqs] Processed 680352 reads in 181.679 CPU sec, 5.869 real sec sorting block 3, #reads = 1075483 mem = 536880927 peak = 875806720 [M::process] read 680814 sequences (100000174 bp) in 1.958 CPU sec, 2.059 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 280306, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 297, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 577) [M::mem_pestat] mean and std.dev: (292.70, 74.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 329, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (343.62, 159.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 227, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (264.47, 135.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 4, #reads = 1076218 mem = 536877774 peak = 875806720 [M::process] read 681686 sequences (100000048 bp) in 1.718 CPU sec, 1.777 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280496, 10, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 339, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (344.77, 184.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 327, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (342.02, 159.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 854, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2651) [M::mem_pestat] mean and std.dev: (623.50, 437.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 214, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (254.76, 100.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681516 sequences (100000072 bp) in 1.310 CPU sec, 1.327 real sec... [M::mem_process_seqs] Processed 680182 reads in 162.761 CPU sec, 5.203 real sec [M::mem_process_seqs] Processed 680814 reads in 105.434 CPU sec, 3.350 real sec sorting block 5, #reads = 1075189 mem = 536880171 peak = 875806720 [M::process] read 681186 sequences (100000159 bp) in 1.809 CPU sec, 1.901 real sec... [M::process] read 681246 sequences (100000083 bp) in 1.398 CPU sec, 1.409 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280470, 8, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 236, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (263.81, 159.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 322, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (337.70, 158.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 217, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (263.10, 107.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681516 reads in 89.960 CPU sec, 2.868 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 280996, 7, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 204, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (200.00, 111.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 320, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (336.48, 158.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 317, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (292.65, 104.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 6, #reads = 1075201 mem = 536878415 peak = 875806720 [M::process] read 681140 sequences (100000089 bp) in 1.434 CPU sec, 1.438 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 280464, 9, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 420, 854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2102) [M::mem_pestat] mean and std.dev: (540.11, 427.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 322, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (337.56, 157.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 245, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (266.66, 122.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681686 reads in 251.628 CPU sec, 8.052 real sec [M::mem_process_seqs] Processed 681186 reads in 122.088 CPU sec, 3.926 real sec sorting block 7, #reads = 1076508 mem = 536881591 peak = 875806720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280628, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 223, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (224.11, 116.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 321, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (337.23, 158.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 292, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (252.18, 74.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680816 sequences (100000028 bp) in 2.641 CPU sec, 2.787 real sec... [M::process] read 680898 sequences (100000190 bp) in 1.443 CPU sec, 1.452 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 279863, 13, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 260, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1517) [M::mem_pestat] mean and std.dev: (361.07, 248.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 320, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (336.25, 157.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (116, 479, 1713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4907) [M::mem_pestat] mean and std.dev: (968.00, 1022.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6504) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 251, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108) [M::mem_pestat] mean and std.dev: (344.19, 286.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681246 reads in 162.211 CPU sec, 5.180 real sec [M::mem_process_seqs] Processed 681140 reads in 104.197 CPU sec, 3.318 real sec sorting block 8, #reads = 1076406 mem = 536880176 peak = 875806720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280653, 4, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 247, 1375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3753) [M::mem_pestat] mean and std.dev: (415.75, 420.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 321, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (337.11, 156.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 375, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (341.33, 175.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680758 sequences (100000259 bp) in 2.142 CPU sec, 2.323 real sec... [M::process] read 682092 sequences (100000291 bp) in 1.489 CPU sec, 1.490 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281187, 4, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 261, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 513) [M::mem_pestat] mean and std.dev: (238.22, 68.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 320, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (335.10, 155.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 308, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1877) [M::mem_pestat] mean and std.dev: (533.66, 465.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680816 reads in 164.590 CPU sec, 5.272 real sec [M::mem_process_seqs] Processed 680898 reads in 103.403 CPU sec, 3.304 real sec sorting block 9, #reads = 1075448 mem = 536882429 peak = 875806720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280710, 12, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 189, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (230.32, 121.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 317, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (332.56, 153.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 654, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331) [M::mem_pestat] mean and std.dev: (491.50, 147.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 234, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (215.63, 91.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682106 sequences (100000094 bp) in 2.849 CPU sec, 2.992 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 280992, 17, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 276, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560) [M::mem_pestat] mean and std.dev: (478.52, 358.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 309, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (323.76, 148.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1420, 3960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10534) [M::mem_pestat] mean and std.dev: (2001.06, 1866.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (222.33, 97.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681254 sequences (100000115 bp) in 1.718 CPU sec, 1.721 real sec... [M::mem_process_seqs] Processed 680758 reads in 166.658 CPU sec, 5.309 real sec sorting block 10, #reads = 1078202 mem = 536878375 peak = 875806720 [M::mem_process_seqs] Processed 682092 reads in 108.731 CPU sec, 3.452 real sec sorting block 11, #reads = 1074954 mem = 536880075 peak = 875806720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281132, 16, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 249, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 453) [M::mem_pestat] mean and std.dev: (227.40, 75.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 310, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (325.07, 149.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 435, 2273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6327) [M::mem_pestat] mean and std.dev: (961.38, 1020.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 227, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (240.97, 89.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680858 sequences (100000059 bp) in 2.286 CPU sec, 2.417 real sec... [M::process] read 680984 sequences (100000272 bp) in 1.830 CPU sec, 1.827 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280348, 1, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 240, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (231.52, 81.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 313, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (330.04, 152.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 325, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1119) [M::mem_pestat] mean and std.dev: (307.00, 162.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682106 reads in 172.857 CPU sec, 5.519 real sec [M::mem_process_seqs] Processed 681254 reads in 107.342 CPU sec, 3.428 real sec sorting block 12, #reads = 1072461 mem = 536881019 peak = 875806720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280417, 3, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 263, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (327.13, 196.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 313, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (330.04, 153.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (209.63, 103.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680922 sequences (100000067 bp) in 2.376 CPU sec, 2.503 real sec... [M::process] read 680580 sequences (100000061 bp) in 1.536 CPU sec, 1.545 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280477, 6, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 273, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (260.20, 120.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 312, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (328.99, 152.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 236, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (293.45, 162.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680858 reads in 163.651 CPU sec, 5.235 real sec [M::mem_process_seqs] Processed 680984 reads in 104.657 CPU sec, 3.346 real sec sorting block 13, #reads = 1076121 mem = 536885806 peak = 875806720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 280301, 11, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 266, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2285) [M::mem_pestat] mean and std.dev: (413.82, 346.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 314, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (330.33, 152.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2, 920, 1811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5429) [M::mem_pestat] mean and std.dev: (1257.55, 1226.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 254, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (251.28, 125.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680632 sequences (100000134 bp) in 2.483 CPU sec, 2.625 real sec... [M::process] read 680328 sequences (100000165 bp) in 1.860 CPU sec, 1.855 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280132, 5, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 321, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (406.00, 365.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 316, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (332.58, 153.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 370, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427) [M::mem_pestat] mean and std.dev: (444.67, 271.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680922 reads in 169.555 CPU sec, 5.414 real sec [M::mem_process_seqs] Processed 680580 reads in 105.454 CPU sec, 3.354 real sec sorting block 14, #reads = 1075879 mem = 536879767 peak = 875806720 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280447, 6, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 443, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3006) [M::mem_pestat] mean and std.dev: (513.39, 468.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 315, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (330.87, 152.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 319, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3414) [M::mem_pestat] mean and std.dev: (683.09, 670.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680172 sequences (100000062 bp) in 2.584 CPU sec, 2.706 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280256, 11, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 339, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (291.44, 160.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 317, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (332.50, 152.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (114, 202, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (204.00, 132.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 200, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (228.90, 108.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680542 sequences (100000020 bp) in 1.657 CPU sec, 1.659 real sec... [M::mem_process_seqs] Processed 680632 reads in 156.522 CPU sec, 4.977 real sec sorting block 15, #reads = 1075164 mem = 536880938 peak = 875806720 [M::mem_process_seqs] Processed 680328 reads in 99.954 CPU sec, 3.169 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279657, 6, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 342, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1873) [M::mem_pestat] mean and std.dev: (412.18, 360.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 318, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (333.48, 153.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 238, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (223.18, 70.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 16, #reads = 1074972 mem = 536880298 peak = 938631168 [M::process] read 682166 sequences (100000052 bp) in 2.491 CPU sec, 2.608 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 280433, 8, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 327, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372) [M::mem_pestat] mean and std.dev: (375.38, 178.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 317, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (333.26, 154.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 340, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253) [M::mem_pestat] mean and std.dev: (365.79, 258.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681860 sequences (100000222 bp) in 1.457 CPU sec, 1.468 real sec... [M::mem_process_seqs] Processed 680172 reads in 153.811 CPU sec, 4.898 real sec [M::mem_process_seqs] Processed 680542 reads in 100.188 CPU sec, 3.184 real sec sorting block 17, #reads = 1074986 mem = 536879547 peak = 938631168 [M::process] read 681514 sequences (100000260 bp) in 2.790 CPU sec, 2.906 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281335, 12, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 194, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (210.10, 86.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 315, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (331.90, 155.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (905, 2213, 3182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7736) [M::mem_pestat] mean and std.dev: (2185.42, 1335.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10013) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 301, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (284.02, 98.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681860 reads in 84.410 CPU sec, 2.651 real sec [M::process] read 680920 sequences (100000105 bp) in 1.919 CPU sec, 1.933 real sec... sorting block 18, #reads = 1076413 mem = 536879720 peak = 938631168 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281831, 17, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 212, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134) [M::mem_pestat] mean and std.dev: (292.67, 172.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 312, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (327.86, 153.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (745, 1067, 2582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6256) [M::mem_pestat] mean and std.dev: (1522.24, 929.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8093) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 299, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (312.52, 132.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1486) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281239, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 527, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1520) [M::mem_pestat] mean and std.dev: (491.55, 337.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 319, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (334.13, 156.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 434, 3915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11603) [M::mem_pestat] mean and std.dev: (1331.80, 1697.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 264, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (290.02, 117.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682166 reads in 236.582 CPU sec, 7.508 real sec [M::process] read 680856 sequences (100000088 bp) in 1.972 CPU sec, 1.998 real sec... [M::mem_process_seqs] Processed 681514 reads in 114.086 CPU sec, 3.634 real sec sorting block 19, #reads = 1076342 mem = 536876960 peak = 938631168 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280628, 11, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 188, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (272.04, 193.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 323, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (339.00, 158.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 736, 2796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7802) [M::mem_pestat] mean and std.dev: (1032.36, 1109.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 303, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (359.42, 193.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680640 sequences (100000282 bp) in 2.756 CPU sec, 2.898 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281018, 8, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 317, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (285.72, 116.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 325, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (340.67, 160.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 242, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (261.91, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680920 reads in 155.660 CPU sec, 4.960 real sec [M::process] read 680398 sequences (100000115 bp) in 1.654 CPU sec, 1.673 real sec... [M::mem_process_seqs] Processed 680856 reads in 102.129 CPU sec, 3.223 real sec sorting block 20, #reads = 1077478 mem = 536878545 peak = 938631168 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280431, 17, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 374, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4263) [M::mem_pestat] mean and std.dev: (807.19, 871.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 327, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (341.91, 159.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (383, 1302, 2386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6392) [M::mem_pestat] mean and std.dev: (1347.88, 894.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8395) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 355, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (290.38, 128.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680050 sequences (100000094 bp) in 2.383 CPU sec, 2.531 real sec... [M::process] read 679952 sequences (100000059 bp) in 1.398 CPU sec, 1.404 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280680, 12, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 291, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (295.14, 160.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 328, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (343.00, 159.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 1821, 1821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5341) [M::mem_pestat] mean and std.dev: (1190.83, 1129.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 214, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.58, 91.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680640 reads in 151.185 CPU sec, 4.815 real sec [M::mem_process_seqs] Processed 680398 reads in 97.623 CPU sec, 3.107 real sec sorting block 21, #reads = 1076791 mem = 536881283 peak = 938631168 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280319, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 293, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (290.28, 111.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (217, 330, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (344.34, 159.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 421, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1962) [M::mem_pestat] mean and std.dev: (438.70, 340.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2536) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679920 sequences (100000175 bp) in 2.416 CPU sec, 2.531 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280295, 7, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 251, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (264.78, 87.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 333, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (347.69, 162.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 255, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (340.45, 198.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681110 sequences (100000169 bp) in 1.508 CPU sec, 1.516 real sec... [M::mem_process_seqs] Processed 680050 reads in 150.642 CPU sec, 4.791 real sec sorting block 22, #reads = 1078129 mem = 536885272 peak = 938631168 [M::mem_process_seqs] Processed 679952 reads in 97.062 CPU sec, 3.078 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 280397, 3, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 221, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (262.63, 138.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (219, 334, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (347.42, 161.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 291, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (264.67, 116.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 23, #reads = 1076657 mem = 536879444 peak = 941154304 [M::process] read 681314 sequences (100000026 bp) in 2.434 CPU sec, 2.563 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280565, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 307, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.16, 122.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 322, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (339.53, 160.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 325, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (289.30, 125.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681154 sequences (100000283 bp) in 1.588 CPU sec, 1.598 real sec... [M::mem_process_seqs] Processed 679920 reads in 154.699 CPU sec, 4.920 real sec [M::mem_process_seqs] Processed 681110 reads in 103.691 CPU sec, 3.289 real sec sorting block 24, #reads = 1077109 mem = 536879882 peak = 941154304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280890, 13, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 256, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531) [M::mem_pestat] mean and std.dev: (356.69, 344.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 322, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (338.62, 159.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (106, 496, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (311.73, 202.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 324, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1927) [M::mem_pestat] mean and std.dev: (396.00, 344.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2500) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681342 sequences (100000208 bp) in 2.639 CPU sec, 2.760 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280726, 20, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 229, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (271.81, 149.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 321, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (337.40, 158.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 648, 1849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4251) [M::mem_pestat] mean and std.dev: (1100.45, 977.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 307, 1347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3609) [M::mem_pestat] mean and std.dev: (514.05, 545.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681108 sequences (100000124 bp) in 1.625 CPU sec, 1.633 real sec... [M::mem_process_seqs] Processed 681314 reads in 154.924 CPU sec, 4.918 real sec [M::mem_process_seqs] Processed 681154 reads in 98.541 CPU sec, 3.124 real sec sorting block 25, #reads = 1077466 mem = 536879737 peak = 941154304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280523, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 237, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (257.60, 120.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 320, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (337.28, 158.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 288, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (286.36, 127.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681028 sequences (100000064 bp) in 2.488 CPU sec, 2.612 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 280405, 16, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 262, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (370.03, 260.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 319, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (335.72, 157.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 86, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (220.88, 220.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 289, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (289.12, 110.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681174 sequences (100000144 bp) in 1.510 CPU sec, 1.510 real sec... [M::mem_process_seqs] Processed 681342 reads in 153.178 CPU sec, 4.874 real sec sorting block 26, #reads = 1077031 mem = 536879540 peak = 941154304 [M::mem_process_seqs] Processed 681108 reads in 99.449 CPU sec, 3.151 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280403, 9, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 237, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (285.43, 130.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 318, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (335.07, 156.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 225, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (244.18, 112.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 27, #reads = 1076234 mem = 536884348 peak = 941154304 [M::process] read 681476 sequences (100000020 bp) in 2.529 CPU sec, 2.647 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280793, 10, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (242.80, 156.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 317, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (333.26, 155.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 393, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3146) [M::mem_pestat] mean and std.dev: (663.60, 621.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 239, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (244.48, 81.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681622 sequences (100000226 bp) in 1.699 CPU sec, 1.703 real sec... [M::mem_process_seqs] Processed 681028 reads in 156.487 CPU sec, 4.973 real sec [M::mem_process_seqs] Processed 681174 reads in 103.553 CPU sec, 3.290 real sec sorting block 28, #reads = 1075990 mem = 536878054 peak = 941154304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281044, 10, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 394, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2441) [M::mem_pestat] mean and std.dev: (542.32, 626.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 314, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (331.64, 155.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 850, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3300) [M::mem_pestat] mean and std.dev: (708.89, 458.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 284, 861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2233) [M::mem_pestat] mean and std.dev: (500.81, 488.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682732 sequences (100000050 bp) in 2.621 CPU sec, 2.728 real sec... [M::process] read 681212 sequences (100000233 bp) in 1.540 CPU sec, 1.548 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 280518, 16, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 297, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (325.28, 145.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 312, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (330.24, 154.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 970, 1745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3969) [M::mem_pestat] mean and std.dev: (1146.40, 818.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5081) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 318, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2403) [M::mem_pestat] mean and std.dev: (544.05, 505.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681476 reads in 163.455 CPU sec, 5.203 real sec [M::mem_process_seqs] Processed 681622 reads in 105.352 CPU sec, 3.350 real sec sorting block 29, #reads = 1075999 mem = 536879595 peak = 941154304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281222, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 209, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (266.84, 164.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 307, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (323.91, 151.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 319, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (295.81, 114.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681258 sequences (100000297 bp) in 2.380 CPU sec, 2.503 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280575, 7, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 295, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (300.97, 155.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 314, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (331.64, 155.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 355, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (319.09, 98.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681068 sequences (100000281 bp) in 1.939 CPU sec, 1.940 real sec... [M::mem_process_seqs] Processed 682732 reads in 174.559 CPU sec, 5.560 real sec [M::mem_process_seqs] Processed 681212 reads in 108.285 CPU sec, 3.434 real sec sorting block 30, #reads = 1072184 mem = 536877797 peak = 941154304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281287, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 439, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2950) [M::mem_pestat] mean and std.dev: (812.42, 804.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 316, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (333.10, 155.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (314.96, 131.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680886 sequences (100000079 bp) in 2.400 CPU sec, 2.501 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281052, 10, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 351, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (337.38, 187.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 314, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (331.81, 155.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 455, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038) [M::mem_pestat] mean and std.dev: (482.50, 431.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2669) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 202, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (263.12, 165.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680636 sequences (100000261 bp) in 1.480 CPU sec, 1.482 real sec... [M::mem_process_seqs] Processed 681258 reads in 159.094 CPU sec, 5.078 real sec sorting block 31, #reads = 1075892 mem = 536876332 peak = 941154304 [M::mem_process_seqs] Processed 681068 reads in 101.996 CPU sec, 3.237 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281155, 8, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 241, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (264.24, 104.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 315, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (332.22, 154.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 323, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (272.11, 121.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1657) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 32, #reads = 1077696 mem = 536880904 peak = 968454144 [M::process] read 680672 sequences (100000299 bp) in 2.892 CPU sec, 3.013 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280663, 7, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 226, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1439) [M::mem_pestat] mean and std.dev: (305.70, 245.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 315, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (331.57, 153.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 298, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (401.74, 251.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1409) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680568 sequences (100000275 bp) in 1.638 CPU sec, 1.641 real sec... [M::mem_process_seqs] Processed 680886 reads in 161.237 CPU sec, 5.130 real sec [M::mem_process_seqs] Processed 680636 reads in 106.100 CPU sec, 3.357 real sec sorting block 33, #reads = 1076025 mem = 536877040 peak = 968454144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280594, 4, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 331, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2872) [M::mem_pestat] mean and std.dev: (420.25, 399.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 316, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (332.17, 153.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 323, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998) [M::mem_pestat] mean and std.dev: (371.52, 201.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680066 sequences (100000221 bp) in 2.393 CPU sec, 2.497 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280953, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 230, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (265.69, 174.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 317, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (333.24, 154.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 315, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452) [M::mem_pestat] mean and std.dev: (361.96, 205.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680164 sequences (100000046 bp) in 1.558 CPU sec, 1.559 real sec... [M::mem_process_seqs] Processed 680672 reads in 160.471 CPU sec, 5.123 real sec [M::mem_process_seqs] Processed 680568 reads in 103.624 CPU sec, 3.291 real sec sorting block 34, #reads = 1075026 mem = 536877859 peak = 968454144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280355, 4, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 316, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (306.85, 122.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 320, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (336.29, 155.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 286, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (307.31, 125.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681998 sequences (100000018 bp) in 2.251 CPU sec, 2.351 real sec... [M::process] read 682130 sequences (100000210 bp) in 1.498 CPU sec, 1.498 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 280762, 10, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 226, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (244.00, 83.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 322, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (338.15, 157.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2449, 2463, 2463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2421, 2491) [M::mem_pestat] mean and std.dev: (2461.25, 4.63) [M::mem_pestat] low and high boundaries for proper pairs: (2407, 2505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 281, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (286.68, 165.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680066 reads in 157.352 CPU sec, 5.026 real sec [M::mem_process_seqs] Processed 680164 reads in 100.475 CPU sec, 3.202 real sec sorting block 35, #reads = 1076087 mem = 536877504 peak = 968454144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280644, 7, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 314, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1904) [M::mem_pestat] mean and std.dev: (416.24, 310.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 314, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (331.32, 155.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 320, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 674) [M::mem_pestat] mean and std.dev: (306.82, 85.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681556 sequences (100000270 bp) in 2.148 CPU sec, 2.252 real sec... [M::process] read 680842 sequences (100000297 bp) in 1.498 CPU sec, 1.503 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 281646, 21, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 322, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (263.78, 95.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 314, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (330.92, 155.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 725, 5008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13754) [M::mem_pestat] mean and std.dev: (1735.10, 1992.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18127) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 253, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (300.58, 173.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681998 reads in 160.709 CPU sec, 5.137 real sec sorting block 36, #reads = 1075003 mem = 536878113 peak = 968454144 [M::process] read 680598 sequences (100000279 bp) in 1.749 CPU sec, 1.756 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281662, 13, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (217.33, 100.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 319, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (335.46, 157.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (132, 546, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7560) [M::mem_pestat] mean and std.dev: (1404.69, 1866.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 265, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (281.95, 108.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681556 reads in 92.329 CPU sec, 2.940 real sec sorting block 37, #reads = 1075684 mem = 536877847 peak = 985784320 [M::mem_process_seqs] Processed 682130 reads in 229.877 CPU sec, 7.340 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281000, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 351, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (319.08, 198.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 322, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (337.35, 157.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 424, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1251) [M::mem_pestat] mean and std.dev: (352.12, 173.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1614) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680280 sequences (100000010 bp) in 2.278 CPU sec, 2.407 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 279971, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 297, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (274.84, 96.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 325, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (340.49, 159.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 228, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (216.09, 67.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680842 reads in 170.848 CPU sec, 5.442 real sec [M::mem_process_seqs] Processed 680598 reads in 100.401 CPU sec, 3.172 real sec sorting block 38, #reads = 1076302 mem = 536877811 peak = 985784320 [M::process] read 680072 sequences (100000039 bp) in 2.203 CPU sec, 2.255 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280822, 11, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (206.62, 105.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 329, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (343.05, 159.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 107, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1400) [M::mem_pestat] mean and std.dev: (156.30, 176.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 292, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (315.63, 183.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 39, #reads = 1076175 mem = 536879620 peak = 995569664 [M::process] read 679590 sequences (100000002 bp) in 2.227 CPU sec, 2.359 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280863, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 264, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (292.18, 153.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 330, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (344.77, 160.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 267, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (238.78, 107.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679814 sequences (100000209 bp) in 1.579 CPU sec, 1.581 real sec... [M::mem_process_seqs] Processed 680280 reads in 156.193 CPU sec, 4.977 real sec [M::mem_process_seqs] Processed 680072 reads in 101.187 CPU sec, 3.215 real sec sorting block 40, #reads = 1075451 mem = 536877990 peak = 995569664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280560, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 300, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (298.75, 143.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 333, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (346.85, 160.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 448, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1457) [M::mem_pestat] mean and std.dev: (403.43, 203.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679488 sequences (100000066 bp) in 2.217 CPU sec, 2.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280702, 10, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 344, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (302.11, 116.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 336, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (350.91, 164.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (753, 2206, 2206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5112) [M::mem_pestat] mean and std.dev: (1605.00, 1136.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 294, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (282.10, 131.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681026 sequences (100000092 bp) in 1.490 CPU sec, 1.489 real sec... [M::mem_process_seqs] Processed 679590 reads in 154.293 CPU sec, 4.930 real sec [M::mem_process_seqs] Processed 679814 reads in 100.313 CPU sec, 3.187 real sec sorting block 41, #reads = 1075985 mem = 536878974 peak = 995569664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 280661, 12, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 255, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (246.90, 63.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 337, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (351.30, 163.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 398, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2330) [M::mem_pestat] mean and std.dev: (596.83, 621.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 277, 861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2243) [M::mem_pestat] mean and std.dev: (479.19, 460.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681576 sequences (100000266 bp) in 2.638 CPU sec, 2.740 real sec... [M::process] read 681040 sequences (100000246 bp) in 1.450 CPU sec, 1.451 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281081, 10, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 263, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (271.82, 141.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 325, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (342.13, 161.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 401, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7835) [M::mem_pestat] mean and std.dev: (960.60, 1203.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10444) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 285, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634) [M::mem_pestat] mean and std.dev: (333.73, 205.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679488 reads in 158.759 CPU sec, 5.050 real sec [M::mem_process_seqs] Processed 681026 reads in 102.128 CPU sec, 3.242 real sec sorting block 42, #reads = 1074904 mem = 536880410 peak = 995569664 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280504, 14, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 376, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3244) [M::mem_pestat] mean and std.dev: (670.27, 676.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 319, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (336.36, 158.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 2699, 7377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20851) [M::mem_pestat] mean and std.dev: (3555.14, 2957.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 27588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 358, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (303.27, 123.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680592 sequences (100000096 bp) in 2.236 CPU sec, 2.351 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 280995, 8, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 215, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273) [M::mem_pestat] mean and std.dev: (474.86, 538.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 323, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (339.42, 159.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 264, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (293.56, 139.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680782 sequences (100000034 bp) in 1.820 CPU sec, 1.823 real sec... [M::mem_process_seqs] Processed 681576 reads in 159.791 CPU sec, 5.089 real sec sorting block 43, #reads = 1076418 mem = 536878089 peak = 995569664 [M::mem_process_seqs] Processed 681040 reads in 102.485 CPU sec, 3.248 real sec sorting block 44, #reads = 1076102 mem = 536879470 peak = 1042509824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 280792, 8, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (209.62, 107.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 323, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (340.53, 159.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 215, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (204.84, 69.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680764 sequences (100000301 bp) in 2.610 CPU sec, 2.704 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280769, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 271, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (272.92, 123.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 320, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (337.65, 159.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 251, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (341.10, 219.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1439) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681120 sequences (100000118 bp) in 1.810 CPU sec, 1.811 real sec... [M::mem_process_seqs] Processed 680592 reads in 163.908 CPU sec, 5.202 real sec [M::mem_process_seqs] Processed 680782 reads in 106.633 CPU sec, 3.375 real sec sorting block 45, #reads = 1076256 mem = 536879069 peak = 1042509824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 280580, 6, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 353, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1969) [M::mem_pestat] mean and std.dev: (471.35, 419.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 320, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (337.74, 159.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 247, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (275.62, 152.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681178 sequences (100000193 bp) in 2.426 CPU sec, 2.515 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280724, 15, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 311, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (338.22, 207.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 318, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (335.50, 157.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (243, 323, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (275.62, 138.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 418, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418) [M::mem_pestat] mean and std.dev: (456.03, 277.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681632 sequences (100000062 bp) in 1.773 CPU sec, 1.783 real sec... [M::mem_process_seqs] Processed 680764 reads in 159.166 CPU sec, 5.055 real sec [M::mem_process_seqs] Processed 681120 reads in 101.691 CPU sec, 3.212 real sec sorting block 46, #reads = 1075644 mem = 536878259 peak = 1042509824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280774, 12, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 297, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2233) [M::mem_pestat] mean and std.dev: (395.21, 359.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 315, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (333.63, 157.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1360, 2369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5909) [M::mem_pestat] mean and std.dev: (1420.42, 895.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 409, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (322.09, 138.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681522 sequences (100000085 bp) in 2.361 CPU sec, 2.446 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280856, 6, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 266, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303) [M::mem_pestat] mean and std.dev: (303.29, 207.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 315, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (332.96, 156.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 266, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (237.89, 75.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681158 sequences (100000060 bp) in 1.790 CPU sec, 1.793 real sec... [M::mem_process_seqs] Processed 681178 reads in 158.265 CPU sec, 5.030 real sec sorting block 47, #reads = 1075908 mem = 536876422 peak = 1042509824 [M::mem_process_seqs] Processed 681632 reads in 102.968 CPU sec, 3.255 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 281251, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 240, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281) [M::mem_pestat] mean and std.dev: (342.31, 247.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 313, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (330.46, 155.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 468, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405) [M::mem_pestat] mean and std.dev: (423.37, 243.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 48, #reads = 1076410 mem = 536877958 peak = 1042509824 [M::process] read 680908 sequences (100000171 bp) in 2.263 CPU sec, 2.361 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280538, 10, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 226, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2834) [M::mem_pestat] mean and std.dev: (568.06, 656.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 315, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (333.30, 156.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1167, 2926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7470) [M::mem_pestat] mean and std.dev: (1604.30, 1184.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 314, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (268.44, 86.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680966 sequences (100000162 bp) in 1.780 CPU sec, 1.784 real sec... [M::mem_process_seqs] Processed 681522 reads in 160.599 CPU sec, 5.117 real sec [M::mem_process_seqs] Processed 681158 reads in 105.046 CPU sec, 3.334 real sec sorting block 49, #reads = 1074581 mem = 536880771 peak = 1042509824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 280552, 9, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 167, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217) [M::mem_pestat] mean and std.dev: (278.43, 193.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 317, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (334.91, 158.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 254, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (102, 402) [M::mem_pestat] mean and std.dev: (242.50, 52.84) [M::mem_pestat] low and high boundaries for proper pairs: (31, 462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680964 sequences (100000178 bp) in 2.645 CPU sec, 2.739 real sec... [M::process] read 680724 sequences (100000216 bp) in 1.550 CPU sec, 1.555 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280815, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272) [M::mem_pestat] mean and std.dev: (319.45, 206.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 318, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (335.95, 158.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 267, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (262.91, 129.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680908 reads in 164.608 CPU sec, 5.242 real sec [M::mem_process_seqs] Processed 680966 reads in 106.142 CPU sec, 3.369 real sec sorting block 50, #reads = 1075022 mem = 536877301 peak = 1042509824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280176, 5, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 285, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (372.08, 231.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 316, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (333.92, 156.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 275, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 540) [M::mem_pestat] mean and std.dev: (256.00, 71.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680630 sequences (100000053 bp) in 2.178 CPU sec, 2.263 real sec... [M::process] read 680402 sequences (100000281 bp) in 1.430 CPU sec, 1.432 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280332, 19, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 254, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2223) [M::mem_pestat] mean and std.dev: (523.86, 595.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 317, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (334.82, 157.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1475, 2247, 4829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11537) [M::mem_pestat] mean and std.dev: (2602.95, 1724.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 306, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (328.29, 159.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680964 reads in 158.879 CPU sec, 5.077 real sec [M::mem_process_seqs] Processed 680724 reads in 102.176 CPU sec, 3.268 real sec sorting block 51, #reads = 1076465 mem = 536878406 peak = 1042509824 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280915, 18, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 317, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (302.71, 160.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 319, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (336.16, 157.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (3, 45, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461) [M::mem_pestat] mean and std.dev: (157.67, 207.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 302, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (277.80, 128.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680668 sequences (100000171 bp) in 2.579 CPU sec, 2.736 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280986, 4, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 300, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (280.88, 190.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 319, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (336.08, 156.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 361, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (318.25, 154.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680206 sequences (100000008 bp) in 1.620 CPU sec, 1.616 real sec... [M::mem_process_seqs] Processed 680630 reads in 163.032 CPU sec, 5.281 real sec sorting block 52, #reads = 1075516 mem = 536877608 peak = 1042509824 [M::mem_process_seqs] Processed 680402 reads in 106.312 CPU sec, 3.404 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280917, 16, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 216, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (212.34, 106.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 317, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (335.01, 157.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 1656, 1836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5328) [M::mem_pestat] mean and std.dev: (1062.94, 935.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7074) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 275, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (274.62, 141.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 53, #reads = 1075564 mem = 536881784 peak = 1069780992 [M::process] read 680660 sequences (100000180 bp) in 2.472 CPU sec, 2.657 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280240, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (227.38, 95.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 322, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (338.52, 158.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 303, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (277.61, 115.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680668 reads in 164.109 CPU sec, 5.325 real sec [M::process] read 681856 sequences (100000202 bp) in 1.763 CPU sec, 1.779 real sec... [M::mem_process_seqs] Processed 680206 reads in 105.695 CPU sec, 3.394 real sec sorting block 54, #reads = 1075133 mem = 536878594 peak = 1069780992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280836, 9, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 327, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455) [M::mem_pestat] mean and std.dev: (308.16, 232.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 318, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (334.59, 155.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 308, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (338.36, 175.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681210 sequences (100000267 bp) in 2.500 CPU sec, 2.680 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280927, 20, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 221, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207) [M::mem_pestat] mean and std.dev: (325.15, 293.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 314, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (331.65, 156.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 1472, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4364) [M::mem_pestat] mean and std.dev: (749.00, 723.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 254, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (226.17, 83.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680970 sequences (100000176 bp) in 1.782 CPU sec, 1.789 real sec... [M::mem_process_seqs] Processed 680660 reads in 165.154 CPU sec, 5.278 real sec [M::mem_process_seqs] Processed 681856 reads in 107.055 CPU sec, 3.402 real sec sorting block 55, #reads = 1074390 mem = 536881901 peak = 1069780992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280346, 4, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 254, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (358.16, 302.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 319, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (336.64, 158.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 217, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (333.48, 238.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680508 sequences (100000253 bp) in 2.150 CPU sec, 2.288 real sec... [M::process] read 680410 sequences (100000223 bp) in 1.572 CPU sec, 1.586 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280537, 8, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 385, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 687) [M::mem_pestat] mean and std.dev: (318.96, 107.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 322, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (339.05, 159.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 414, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (505.11, 390.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681210 reads in 159.872 CPU sec, 5.142 real sec [M::mem_process_seqs] Processed 680970 reads in 104.403 CPU sec, 3.379 real sec sorting block 56, #reads = 1076349 mem = 536879900 peak = 1069780992 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280722, 5, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 269, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (252.46, 92.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 326, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (342.48, 161.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 244, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (42, 432) [M::mem_pestat] mean and std.dev: (247.44, 79.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680242 sequences (100000188 bp) in 2.446 CPU sec, 2.624 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280074, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 298, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (287.19, 138.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 327, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (343.40, 161.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 251, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (380.83, 286.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679900 sequences (100000083 bp) in 1.647 CPU sec, 1.678 real sec... [M::mem_process_seqs] Processed 680508 reads in 159.636 CPU sec, 5.230 real sec sorting block 57, #reads = 1075742 mem = 536879083 peak = 1075916800 [M::mem_process_seqs] Processed 680410 reads in 108.127 CPU sec, 3.546 real sec sorting block 58, #reads = 1076874 mem = 536878665 peak = 1105158144 [M::process] read 679410 sequences (100000166 bp) in 2.355 CPU sec, 2.539 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 279962, 9, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574) [M::mem_pestat] mean and std.dev: (370.96, 233.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (218, 331, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (345.76, 160.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 259, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (360.28, 291.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 679410 sequences (100000022 bp) in 1.458 CPU sec, 1.524 real sec... [M::mem_process_seqs] Processed 679900 reads in 95.422 CPU sec, 3.069 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280428, 2, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 349, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (294.17, 143.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 328, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (344.15, 161.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (335.03, 232.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279810, 10, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 286, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3590) [M::mem_pestat] mean and std.dev: (642.45, 724.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (220, 334, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (348.99, 161.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (105, 354, 1762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5076) [M::mem_pestat] mean and std.dev: (710.70, 718.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6733) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 261, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (342.76, 212.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680242 reads in 250.553 CPU sec, 8.158 real sec [M::process] read 679488 sequences (100000176 bp) in 2.030 CPU sec, 2.052 real sec... sorting block 59, #reads = 1077037 mem = 536876945 peak = 1105158144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280318, 21, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (280.31, 149.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 336, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (350.75, 162.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (875, 1894, 2530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5840) [M::mem_pestat] mean and std.dev: (1622.76, 999.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 281, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3306) [M::mem_pestat] mean and std.dev: (510.16, 457.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679410 reads in 89.334 CPU sec, 3.077 real sec [M::process] read 681024 sequences (100000052 bp) in 2.052 CPU sec, 2.151 real sec... sorting block 60, #reads = 1075320 mem = 536880617 peak = 1105158144 [M::mem_process_seqs] Processed 679410 reads in 256.244 CPU sec, 8.514 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 280601, 2, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 241, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (294.56, 191.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (221, 337, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (351.66, 163.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 315, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (279.13, 120.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681120 sequences (100000246 bp) in 2.266 CPU sec, 2.563 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280486, 2, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 308, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (298.00, 124.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 323, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (340.57, 161.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 369, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (322.00, 149.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 679488 reads in 179.699 CPU sec, 5.815 real sec [M::mem_process_seqs] Processed 681024 reads in 107.950 CPU sec, 3.460 real sec sorting block 61, #reads = 1076375 mem = 536878201 peak = 1105158144 [M::process] read 680478 sequences (100000253 bp) in 1.823 CPU sec, 1.917 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280563, 5, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 191, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 378) [M::mem_pestat] mean and std.dev: (191.34, 76.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 321, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (339.13, 161.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 328, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (264.15, 119.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680744 sequences (100000129 bp) in 2.289 CPU sec, 2.439 real sec... sorting block 62, #reads = 1076435 mem = 536876531 peak = 1105158144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280000, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 274, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2073) [M::mem_pestat] mean and std.dev: (519.56, 587.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (212, 323, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (340.79, 160.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 328, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (342.47, 170.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680634 sequences (100000280 bp) in 1.357 CPU sec, 1.361 real sec... [M::mem_process_seqs] Processed 681120 reads in 162.485 CPU sec, 5.207 real sec [M::mem_process_seqs] Processed 680478 reads in 107.111 CPU sec, 3.407 real sec sorting block 63, #reads = 1076341 mem = 536879845 peak = 1105158144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280703, 15, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 251, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133) [M::mem_pestat] mean and std.dev: (289.54, 175.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 320, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (338.84, 160.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (516, 1114, 2410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6198) [M::mem_pestat] mean and std.dev: (1072.86, 805.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 192, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (226.50, 124.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680962 sequences (100000075 bp) in 2.264 CPU sec, 2.405 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280375, 8, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 355, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1684) [M::mem_pestat] mean and std.dev: (401.85, 343.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 319, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (336.78, 158.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (308, 492, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (521.88, 290.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681166 sequences (100000074 bp) in 1.739 CPU sec, 1.742 real sec... [M::mem_process_seqs] Processed 680744 reads in 164.638 CPU sec, 5.264 real sec [M::mem_process_seqs] Processed 680634 reads in 107.979 CPU sec, 3.437 real sec sorting block 64, #reads = 1076693 mem = 536880049 peak = 1105158144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280393, 9, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 453, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3453) [M::mem_pestat] mean and std.dev: (794.69, 914.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 318, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (336.06, 158.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 273, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (317.71, 211.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680922 sequences (100000196 bp) in 2.268 CPU sec, 2.474 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279870, 9, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 343, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (292.13, 145.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 316, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (333.87, 156.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 262, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (239.43, 131.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681010 sequences (100000108 bp) in 1.680 CPU sec, 1.686 real sec... [M::mem_process_seqs] Processed 680962 reads in 166.625 CPU sec, 5.448 real sec [M::mem_process_seqs] Processed 681166 reads in 109.513 CPU sec, 3.585 real sec sorting block 65, #reads = 1075862 mem = 536878404 peak = 1105158144 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281006, 4, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 247, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (402.37, 295.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 317, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (334.67, 157.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 286, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (292.81, 127.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680886 sequences (100000245 bp) in 2.388 CPU sec, 2.592 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280749, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 344, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (289.80, 148.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 317, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (333.86, 156.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 366, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (340.85, 180.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1542) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680976 sequences (100000193 bp) in 1.824 CPU sec, 1.840 real sec... [M::mem_process_seqs] Processed 680922 reads in 166.691 CPU sec, 5.362 real sec sorting block 66, #reads = 1075111 mem = 536879282 peak = 1105158144 [M::mem_process_seqs] Processed 681010 reads in 110.089 CPU sec, 3.531 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 280298, 8, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 272, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (257.00, 85.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 318, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (334.88, 156.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 223, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (218.89, 96.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 67, #reads = 1076447 mem = 536882261 peak = 1125658624 [M::process] read 681186 sequences (100000189 bp) in 2.458 CPU sec, 2.655 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280671, 9, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 355, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (347.79, 212.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 317, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (335.37, 157.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (295, 374, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1384) [M::mem_pestat] mean and std.dev: (425.42, 207.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680982 sequences (100000299 bp) in 1.559 CPU sec, 1.579 real sec... [M::mem_process_seqs] Processed 680886 reads in 167.161 CPU sec, 5.380 real sec [M::mem_process_seqs] Processed 680976 reads in 107.607 CPU sec, 3.435 real sec sorting block 68, #reads = 1076277 mem = 536881083 peak = 1125658624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280839, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 342, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (274.28, 208.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 317, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (335.41, 158.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 260, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (265.68, 98.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680950 sequences (100000125 bp) in 2.371 CPU sec, 2.452 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280828, 8, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 188, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (177.22, 58.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 316, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (335.34, 158.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 1416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3818) [M::mem_pestat] mean and std.dev: (593.37, 655.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680506 sequences (100000096 bp) in 1.820 CPU sec, 1.820 real sec... [M::mem_process_seqs] Processed 681186 reads in 165.312 CPU sec, 5.252 real sec [M::mem_process_seqs] Processed 680982 reads in 106.671 CPU sec, 3.375 real sec sorting block 69, #reads = 1076077 mem = 536876620 peak = 1125658624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280645, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 192, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (211.70, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 317, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (334.43, 156.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 309, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2099) [M::mem_pestat] mean and std.dev: (417.50, 399.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680348 sequences (100000192 bp) in 2.189 CPU sec, 2.297 real sec... [M::process] read 679926 sequences (100000177 bp) in 1.849 CPU sec, 1.849 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280549, 6, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 265, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (300.53, 161.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 320, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (336.78, 157.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 332, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (311.28, 133.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680950 reads in 165.319 CPU sec, 5.259 real sec [M::mem_process_seqs] Processed 680506 reads in 106.579 CPU sec, 3.372 real sec sorting block 70, #reads = 1075322 mem = 536880622 peak = 1125658624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 280971, 14, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 351, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1593) [M::mem_pestat] mean and std.dev: (329.36, 191.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (213, 320, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (336.57, 156.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (469, 1044, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3214) [M::mem_pestat] mean and std.dev: (984.54, 833.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 364, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188) [M::mem_pestat] mean and std.dev: (403.14, 226.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680056 sequences (100000112 bp) in 2.619 CPU sec, 2.743 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280660, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 320, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (292.34, 113.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 322, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (338.18, 156.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 211, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215) [M::mem_pestat] mean and std.dev: (381.12, 326.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680348 reads in 164.036 CPU sec, 5.222 real sec [M::process] read 679826 sequences (100000071 bp) in 1.936 CPU sec, 1.965 real sec... sorting block 71, #reads = 1076110 mem = 536880588 peak = 1125658624 [M::mem_process_seqs] Processed 679926 reads in 107.357 CPU sec, 3.390 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281038, 5, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 272, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (76, 421) [M::mem_pestat] mean and std.dev: (246.19, 73.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (214, 323, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (339.30, 157.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 340, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (279.75, 147.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 72, #reads = 1076837 mem = 536884449 peak = 1131433984 [M::process] read 683354 sequences (100000241 bp) in 2.684 CPU sec, 2.799 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 279849, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 295, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (271.17, 87.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 324, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (340.36, 157.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (155, 3605, 3605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10505) [M::mem_pestat] mean and std.dev: (2277.90, 1551.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13955) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 253, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (249.22, 112.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680056 reads in 167.917 CPU sec, 5.352 real sec [M::process] read 685798 sequences (100000164 bp) in 1.917 CPU sec, 1.923 real sec... [M::mem_process_seqs] Processed 679826 reads in 112.207 CPU sec, 3.554 real sec sorting block 73, #reads = 1076083 mem = 536881388 peak = 1131433984 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282172, 8, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 282, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2067) [M::mem_pestat] mean and std.dev: (443.96, 322.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (328.71, 158.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 229, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (244.73, 117.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685110 sequences (100000217 bp) in 2.559 CPU sec, 2.729 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282415, 20, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 213, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (227.92, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (319.77, 157.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 194, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (108.38, 72.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 311, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (271.30, 132.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684962 sequences (100000058 bp) in 1.777 CPU sec, 1.786 real sec... [M::mem_process_seqs] Processed 683354 reads in 182.365 CPU sec, 5.850 real sec [M::mem_process_seqs] Processed 685798 reads in 120.693 CPU sec, 3.843 real sec sorting block 74, #reads = 1074432 mem = 536879278 peak = 1131433984 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281999, 14, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 268, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (222.44, 83.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (323.09, 158.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 446, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3740) [M::mem_pestat] mean and std.dev: (1002.62, 1019.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5081) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 478, 1281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3363) [M::mem_pestat] mean and std.dev: (761.22, 736.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684306 sequences (100000171 bp) in 2.714 CPU sec, 2.874 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282286, 13, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 266, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (264.63, 119.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (323.82, 157.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (467, 945, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1901) [M::mem_pestat] mean and std.dev: (657.25, 332.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 264, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2929) [M::mem_pestat] mean and std.dev: (341.08, 279.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683796 sequences (100000151 bp) in 1.938 CPU sec, 1.947 real sec... [M::mem_process_seqs] Processed 685110 reads in 185.245 CPU sec, 5.933 real sec sorting block 75, #reads = 1075911 mem = 536878075 peak = 1131433984 [M::mem_process_seqs] Processed 684962 reads in 121.977 CPU sec, 3.868 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282470, 28, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 251, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3370) [M::mem_pestat] mean and std.dev: (618.62, 709.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (325.26, 156.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (491, 654, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6818) [M::mem_pestat] mean and std.dev: (1236.14, 1025.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8927) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 413, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (304.56, 182.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 76, #reads = 1075475 mem = 536883123 peak = 1131433984 [M::process] read 682634 sequences (100000108 bp) in 2.548 CPU sec, 2.670 real sec... [M::process] read 682736 sequences (100000263 bp) in 1.794 CPU sec, 1.809 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282324, 1, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 388, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2045) [M::mem_pestat] mean and std.dev: (338.39, 244.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 312, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (330.17, 158.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 273, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (305.18, 173.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684306 reads in 186.185 CPU sec, 5.949 real sec [M::mem_process_seqs] Processed 683796 reads in 120.448 CPU sec, 3.831 real sec sorting block 77, #reads = 1073009 mem = 536878190 peak = 1131433984 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281320, 12, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 246, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (232.68, 106.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (333.55, 157.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 177, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2659) [M::mem_pestat] mean and std.dev: (325.45, 360.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 304, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (287.62, 84.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682780 sequences (100000160 bp) in 2.642 CPU sec, 2.734 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282093, 5, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 261, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1672) [M::mem_pestat] mean and std.dev: (457.33, 437.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 317, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (333.25, 158.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 320, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (287.07, 110.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684664 sequences (100000135 bp) in 1.926 CPU sec, 1.934 real sec... [M::mem_process_seqs] Processed 682634 reads in 178.275 CPU sec, 5.692 real sec [M::mem_process_seqs] Processed 682736 reads in 115.707 CPU sec, 3.663 real sec sorting block 78, #reads = 1073510 mem = 536878431 peak = 1131433984 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281549, 22, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 178, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (267.12, 192.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 318, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (332.94, 157.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1358, 1766, 1766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (542, 2582) [M::mem_pestat] mean and std.dev: (1560.40, 414.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 260, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (266.40, 123.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685456 sequences (100000277 bp) in 2.635 CPU sec, 2.763 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282268, 18, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 327, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (307.30, 134.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (321.41, 154.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (785, 1413, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2669) [M::mem_pestat] mean and std.dev: (1160.72, 557.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 341, 2824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8004) [M::mem_pestat] mean and std.dev: (1480.00, 2174.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684882 sequences (100000156 bp) in 1.968 CPU sec, 1.971 real sec... [M::mem_process_seqs] Processed 682780 reads in 180.823 CPU sec, 5.790 real sec [M::mem_process_seqs] Processed 684664 reads in 119.390 CPU sec, 3.785 real sec sorting block 79, #reads = 1075379 mem = 536877614 peak = 1131433984 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283006, 14, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 303, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761) [M::mem_pestat] mean and std.dev: (384.19, 225.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (319.95, 155.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 962, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2798) [M::mem_pestat] mean and std.dev: (918.07, 837.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 229, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2795) [M::mem_pestat] mean and std.dev: (522.65, 594.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685206 sequences (100000139 bp) in 2.448 CPU sec, 2.575 real sec... [M::process] read 684480 sequences (100000075 bp) in 1.950 CPU sec, 1.956 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282653, 22, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 224, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (253.46, 141.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (320.31, 153.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 1298, 2308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6786) [M::mem_pestat] mean and std.dev: (994.95, 924.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9025) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 277, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (286.92, 111.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685456 reads in 191.458 CPU sec, 6.121 real sec sorting block 80, #reads = 1074817 mem = 536881857 peak = 1131433984 [M::mem_process_seqs] Processed 684882 reads in 126.060 CPU sec, 4.009 real sec sorting block 81, #reads = 1074446 mem = 536877943 peak = 1131433984 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282863, 13, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 298, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (278.28, 96.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (320.81, 154.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 618, 2088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6212) [M::mem_pestat] mean and std.dev: (824.85, 913.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 228, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (241.24, 71.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683652 sequences (100000106 bp) in 2.533 CPU sec, 2.623 real sec... [M::process] read 683198 sequences (100000252 bp) in 1.825 CPU sec, 1.841 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282408, 5, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 379, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (331.76, 121.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 303, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (322.56, 154.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 315, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5217) [M::mem_pestat] mean and std.dev: (813.24, 790.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685206 reads in 194.651 CPU sec, 6.217 real sec [M::mem_process_seqs] Processed 684480 reads in 127.305 CPU sec, 4.050 real sec sorting block 82, #reads = 1072825 mem = 536878815 peak = 1131433984 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281648, 11, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 292, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (263.78, 92.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 307, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (326.50, 155.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 561, 1672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4388) [M::mem_pestat] mean and std.dev: (980.82, 906.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 315, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (384.02, 297.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 685262 sequences (100000002 bp) in 2.473 CPU sec, 2.580 real sec... [M::process] read 684922 sequences (100000070 bp) in 1.754 CPU sec, 1.765 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281286, 7, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 165, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (209.54, 134.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (326.34, 154.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 331, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2753) [M::mem_pestat] mean and std.dev: (600.83, 496.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683652 reads in 188.114 CPU sec, 6.008 real sec [M::mem_process_seqs] Processed 683198 reads in 123.187 CPU sec, 3.920 real sec sorting block 83, #reads = 1072478 mem = 536879191 peak = 1131433984 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282190, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 304, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (338.79, 156.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 299, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (318.24, 152.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 339, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (286.15, 88.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684262 sequences (100000161 bp) in 2.515 CPU sec, 2.595 real sec... [M::process] read 682930 sequences (100000070 bp) in 1.788 CPU sec, 1.792 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282385, 15, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 215, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (208.56, 80.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 298, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (317.62, 150.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (415, 782, 2708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7294) [M::mem_pestat] mean and std.dev: (889.67, 851.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 238, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1863) [M::mem_pestat] mean and std.dev: (379.97, 389.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685262 reads in 209.352 CPU sec, 6.685 real sec sorting block 84, #reads = 1073121 mem = 536881212 peak = 1131433984 [M::mem_process_seqs] Processed 684922 reads in 139.317 CPU sec, 4.445 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282208, 12, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 227, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358) [M::mem_pestat] mean and std.dev: (366.33, 264.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 302, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (320.23, 150.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2377, 2377, 2377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2377, 2377) [M::mem_pestat] mean and std.dev: (2377.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (2377, 2377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 270, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (237.92, 82.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 85, #reads = 1065738 mem = 536877458 peak = 1143771136 [M::process] read 683000 sequences (100000098 bp) in 2.653 CPU sec, 2.743 real sec... [M::process] read 682918 sequences (100000103 bp) in 2.001 CPU sec, 2.003 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282211, 11, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 267, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277) [M::mem_pestat] mean and std.dev: (301.12, 183.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (326.41, 154.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1100, 1850, 5309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13727) [M::mem_pestat] mean and std.dev: (3277.73, 2588.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 308, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125) [M::mem_pestat] mean and std.dev: (305.09, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684262 reads in 203.401 CPU sec, 6.479 real sec [M::mem_process_seqs] Processed 682930 reads in 132.132 CPU sec, 4.201 real sec sorting block 86, #reads = 1068898 mem = 536880237 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281676, 10, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 243, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (201.80, 78.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (326.34, 154.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (304, 786, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1750) [M::mem_pestat] mean and std.dev: (619.11, 438.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 270, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (286.00, 163.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682604 sequences (100000230 bp) in 2.675 CPU sec, 2.783 real sec... [M::process] read 683598 sequences (100000147 bp) in 1.716 CPU sec, 1.721 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281014, 17, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 249, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (252.40, 108.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 311, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (329.29, 156.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (365, 465, 1552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3926) [M::mem_pestat] mean and std.dev: (974.29, 1078.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 248, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (233.55, 108.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683000 reads in 200.700 CPU sec, 6.402 real sec [M::mem_process_seqs] Processed 682918 reads in 132.520 CPU sec, 4.211 real sec sorting block 87, #reads = 1069929 mem = 536876591 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281477, 7, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 319, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (239.92, 105.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 310, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (329.35, 156.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 301, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (430.78, 323.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682756 sequences (100000282 bp) in 2.326 CPU sec, 2.430 real sec... [M::process] read 688558 sequences (100000133 bp) in 1.989 CPU sec, 1.992 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281662, 14, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 257, 1630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4570) [M::mem_pestat] mean and std.dev: (848.56, 922.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 305, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (325.45, 155.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1, 116, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1801) [M::mem_pestat] mean and std.dev: (190.42, 269.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2401) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 266, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (241.36, 111.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682604 reads in 200.853 CPU sec, 6.408 real sec [M::mem_process_seqs] Processed 683598 reads in 133.287 CPU sec, 4.245 real sec sorting block 88, #reads = 1069800 mem = 536879431 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281770, 8, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 219, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (288.18, 215.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 310, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (330.20, 158.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 310, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1655) [M::mem_pestat] mean and std.dev: (491.26, 435.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687684 sequences (100000067 bp) in 2.400 CPU sec, 2.498 real sec... [M::process] read 686072 sequences (100000168 bp) in 1.846 CPU sec, 1.855 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283645, 16, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 277, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477) [M::mem_pestat] mean and std.dev: (345.59, 263.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 280, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (304.66, 150.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (751, 2325, 2955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7363) [M::mem_pestat] mean and std.dev: (1993.00, 1450.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 403, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1521) [M::mem_pestat] mean and std.dev: (351.14, 217.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682756 reads in 205.364 CPU sec, 6.556 real sec sorting block 89, #reads = 1069483 mem = 536878700 peak = 1143771136 [M::mem_process_seqs] Processed 688558 reads in 138.650 CPU sec, 4.419 real sec sorting block 90, #reads = 1069416 mem = 536879507 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283834, 4, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 289, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (257.03, 105.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 284, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (307.35, 149.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 376, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (373.79, 261.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685854 sequences (100000248 bp) in 2.409 CPU sec, 2.521 real sec... [M::process] read 684726 sequences (100000075 bp) in 1.793 CPU sec, 1.812 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283032, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 352, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (315.46, 115.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 294, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (314.93, 152.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 270, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (217.56, 101.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687684 reads in 203.775 CPU sec, 6.507 real sec [M::mem_process_seqs] Processed 686072 reads in 131.770 CPU sec, 4.198 real sec sorting block 91, #reads = 1068662 mem = 536879975 peak = 1143771136 [M::process] read 683882 sequences (100000289 bp) in 2.597 CPU sec, 2.695 real sec... [M::process] read 683454 sequences (100000210 bp) in 1.859 CPU sec, 1.867 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282628, 7, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 437, 2470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6788) [M::mem_pestat] mean and std.dev: (934.72, 1074.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (319.84, 152.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 330, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1860) [M::mem_pestat] mean and std.dev: (476.54, 381.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2422) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684726 reads in 113.159 CPU sec, 3.594 real sec sorting block 92, #reads = 1070307 mem = 536877950 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283273, 5, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 274, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (256.04, 106.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (315.77, 152.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 256, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (265.71, 133.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282270, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 329, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551) [M::mem_pestat] mean and std.dev: (418.71, 280.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 307, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (324.54, 153.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 355, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (340.48, 114.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683382 sequences (100000157 bp) in 1.965 CPU sec, 1.975 real sec... [M::mem_process_seqs] Processed 685854 reads in 306.438 CPU sec, 9.776 real sec [M::mem_process_seqs] Processed 683882 reads in 149.070 CPU sec, 4.765 real sec sorting block 93, #reads = 1070337 mem = 536879605 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 282448, 9, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 200, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (232.00, 136.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 309, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (326.53, 154.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 325, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1398) [M::mem_pestat] mean and std.dev: (340.21, 184.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684870 sequences (100000088 bp) in 2.428 CPU sec, 2.556 real sec... [M::process] read 684332 sequences (100000084 bp) in 1.586 CPU sec, 1.588 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 282844, 13, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 380, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1451) [M::mem_pestat] mean and std.dev: (488.80, 352.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 310, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (327.57, 155.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (435, 2654, 3585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9885) [M::mem_pestat] mean and std.dev: (2481.92, 2187.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13035) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 398, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (310.53, 133.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683454 reads in 188.658 CPU sec, 6.027 real sec [M::mem_process_seqs] Processed 683382 reads in 121.899 CPU sec, 3.887 real sec sorting block 94, #reads = 1070649 mem = 536884438 peak = 1143771136 [M::process] read 684584 sequences (100000217 bp) in 2.630 CPU sec, 2.780 real sec... [M::process] read 684172 sequences (100000051 bp) in 1.920 CPU sec, 1.920 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282843, 8, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 309, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1991) [M::mem_pestat] mean and std.dev: (426.27, 458.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 302, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (321.92, 154.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 251, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (281.97, 133.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684332 reads in 103.730 CPU sec, 3.288 real sec sorting block 95, #reads = 1073788 mem = 536881993 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282146, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 277, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (258.78, 106.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (320.57, 154.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 205, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (222.77, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 282992, 4, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 279, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1909) [M::mem_pestat] mean and std.dev: (470.56, 388.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (321.39, 154.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 302, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (282.26, 90.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684628 sequences (100000137 bp) in 1.994 CPU sec, 2.002 real sec... [M::mem_process_seqs] Processed 684870 reads in 283.064 CPU sec, 8.996 real sec [M::mem_process_seqs] Processed 684584 reads in 138.384 CPU sec, 4.418 real sec sorting block 96, #reads = 1074098 mem = 536879081 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282319, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 213, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (306.68, 222.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 304, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (323.73, 155.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 272, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376) [M::mem_pestat] mean and std.dev: (351.10, 242.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684432 sequences (100000218 bp) in 2.861 CPU sec, 2.982 real sec... [M::process] read 684046 sequences (100000243 bp) in 1.950 CPU sec, 1.946 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282161, 5, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 271, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378) [M::mem_pestat] mean and std.dev: (357.00, 266.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 300, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (321.55, 155.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 275, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (231.41, 66.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684172 reads in 184.290 CPU sec, 5.850 real sec [M::mem_process_seqs] Processed 684628 reads in 120.877 CPU sec, 3.831 real sec sorting block 97, #reads = 1074572 mem = 536878814 peak = 1143771136 [M::process] read 686774 sequences (100000132 bp) in 2.130 CPU sec, 2.224 real sec... [M::process] read 682816 sequences (100000141 bp) in 1.678 CPU sec, 1.689 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282328, 12, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 234, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (283.25, 161.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 305, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (324.84, 155.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (410, 558, 1564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3872) [M::mem_pestat] mean and std.dev: (786.42, 726.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5026) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 253, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279) [M::mem_pestat] mean and std.dev: (384.00, 306.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684046 reads in 111.168 CPU sec, 3.539 real sec sorting block 98, #reads = 1072796 mem = 536880266 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282128, 10, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 214, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (243.55, 123.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 302, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (322.24, 154.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (490, 828, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504) [M::mem_pestat] mean and std.dev: (576.62, 236.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1842) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 242, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (248.73, 114.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682348 sequences (100000242 bp) in 1.807 CPU sec, 1.818 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282732, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 352, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (284.43, 113.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 290, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (310.89, 150.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 276, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (303.94, 180.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684432 reads in 304.877 CPU sec, 9.717 real sec sorting block 99, #reads = 1070628 mem = 536881098 peak = 1143771136 [M::mem_process_seqs] Processed 686774 reads in 242.049 CPU sec, 7.723 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281206, 14, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 244, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (300.41, 140.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 312, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (331.69, 159.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 490, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (252.00, 202.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 369, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (299.03, 149.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682300 sequences (100000000 bp) in 2.782 CPU sec, 2.907 real sec... [M::process] read 682084 sequences (100000201 bp) in 1.840 CPU sec, 1.836 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281617, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 192, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1119) [M::mem_pestat] mean and std.dev: (269.20, 185.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 314, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (334.46, 161.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 319, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (294.11, 135.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682816 reads in 179.083 CPU sec, 5.672 real sec sorting block 100, #reads = 1066031 mem = 536883034 peak = 1143771136 [M::mem_process_seqs] Processed 682348 reads in 115.045 CPU sec, 3.645 real sec sorting block 101, #reads = 1073785 mem = 536879046 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281232, 8, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 374, 1706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4700) [M::mem_pestat] mean and std.dev: (797.64, 703.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (334.41, 161.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 294, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117) [M::mem_pestat] mean and std.dev: (310.32, 206.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681924 sequences (100000181 bp) in 2.408 CPU sec, 2.514 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281686, 11, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 352, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (292.63, 139.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 316, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (336.94, 162.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (720, 853, 2691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6633) [M::mem_pestat] mean and std.dev: (1463.91, 942.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1573) [M::mem_pestat] mean and std.dev: (376.09, 275.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681696 sequences (100000098 bp) in 2.259 CPU sec, 2.259 real sec... [M::mem_process_seqs] Processed 682300 reads in 174.327 CPU sec, 5.514 real sec [M::mem_process_seqs] Processed 682084 reads in 112.197 CPU sec, 3.541 real sec sorting block 102, #reads = 1073492 mem = 536878834 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280985, 13, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 440, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2494) [M::mem_pestat] mean and std.dev: (500.73, 452.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (338.86, 164.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 480, 2422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7170) [M::mem_pestat] mean and std.dev: (1039.31, 1125.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 200, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (201.57, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681830 sequences (100000243 bp) in 2.607 CPU sec, 2.677 real sec... [M::process] read 681686 sequences (100000025 bp) in 1.850 CPU sec, 1.855 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281272, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 211, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (220.00, 93.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 321, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (340.82, 164.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 253, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.28, 95.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681924 reads in 170.213 CPU sec, 5.388 real sec [M::mem_process_seqs] Processed 681696 reads in 111.018 CPU sec, 3.510 real sec sorting block 103, #reads = 1073437 mem = 536882519 peak = 1143771136 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281054, 13, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 360, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1447) [M::mem_pestat] mean and std.dev: (393.00, 281.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (339.00, 164.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 1029, 1117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2459) [M::mem_pestat] mean and std.dev: (830.83, 311.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 342, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631) [M::mem_pestat] mean and std.dev: (375.78, 297.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684408 sequences (100000028 bp) in 2.394 CPU sec, 2.492 real sec... [M::process] read 684800 sequences (100000275 bp) in 1.470 CPU sec, 1.473 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281149, 9, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 401, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428) [M::mem_pestat] mean and std.dev: (371.50, 210.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 319, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (340.24, 165.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 290, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2133) [M::mem_pestat] mean and std.dev: (505.67, 476.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681830 reads in 173.725 CPU sec, 5.519 real sec sorting block 104, #reads = 1073428 mem = 536882284 peak = 1143771136 [M::mem_process_seqs] Processed 681686 reads in 114.129 CPU sec, 3.640 real sec [M::process] read 683846 sequences (100000064 bp) in 2.301 CPU sec, 2.404 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 281885, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324) [M::mem_pestat] mean and std.dev: (400.39, 318.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (325.10, 160.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 284, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (275.22, 125.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683200 sequences (100000023 bp) in 1.420 CPU sec, 1.427 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282355, 5, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 344, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (331.62, 141.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (331.34, 162.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 290, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (269.41, 104.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282560, 2, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (212.71, 75.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (326.45, 162.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 305, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (253.29, 108.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684408 reads in 250.769 CPU sec, 8.015 real sec sorting block 105, #reads = 1070915 mem = 536877994 peak = 1143771136 [M::mem_process_seqs] Processed 683846 reads in 174.925 CPU sec, 5.613 real sec [M::mem_process_seqs] Processed 684800 reads in 191.003 CPU sec, 6.153 real sec sorting block 106, #reads = 1068881 mem = 536878327 peak = 1151156224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282831, 7, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 239, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (247.00, 103.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 313, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (333.57, 162.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 394, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562) [M::mem_pestat] mean and std.dev: (350.32, 248.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683570 sequences (100000192 bp) in 2.544 CPU sec, 2.697 real sec... [M::mem_process_seqs] Processed 683200 reads in 88.741 CPU sec, 2.929 real sec sorting block 107, #reads = 1077468 mem = 536884145 peak = 1151156224 [M::process] read 681866 sequences (100000111 bp) in 2.011 CPU sec, 2.130 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282320, 6, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 214, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (193.79, 93.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 311, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (331.76, 161.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 363, 4721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13889) [M::mem_pestat] mean and std.dev: (1564.64, 2208.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681618 sequences (100000214 bp) in 1.576 CPU sec, 1.605 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281576, 12, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 201, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (204.65, 69.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 320, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (336.90, 160.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (285, 1993, 2402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6636) [M::mem_pestat] mean and std.dev: (1285.42, 1064.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 282, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774) [M::mem_pestat] mean and std.dev: (447.71, 351.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681476 sequences (100000159 bp) in 1.400 CPU sec, 1.399 real sec... [M::mem_process_seqs] Processed 683570 reads in 161.821 CPU sec, 5.132 real sec [M::mem_process_seqs] Processed 681866 reads in 103.149 CPU sec, 3.262 real sec sorting block 108, #reads = 1076265 mem = 536880280 peak = 1151156224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281242, 10, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 261, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (277.53, 150.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 322, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (339.14, 161.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 397, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (369, 439) [M::mem_pestat] mean and std.dev: (398.75, 4.63) [M::mem_pestat] low and high boundaries for proper pairs: (355, 453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 277, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (281.97, 135.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681598 sequences (100000150 bp) in 2.218 CPU sec, 2.337 real sec... [M::process] read 682030 sequences (100000105 bp) in 1.800 CPU sec, 1.802 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281514, 10, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 272, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (221.38, 84.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 324, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (340.58, 161.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1767, 2407, 2711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4599) [M::mem_pestat] mean and std.dev: (2363.50, 911.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 245, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (238.97, 93.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681618 reads in 158.933 CPU sec, 5.049 real sec sorting block 109, #reads = 1077266 mem = 536878899 peak = 1151156224 [M::mem_process_seqs] Processed 681476 reads in 102.828 CPU sec, 3.275 real sec sorting block 110, #reads = 1076689 mem = 536880060 peak = 1151156224 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281841, 13, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 217, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1930) [M::mem_pestat] mean and std.dev: (424.33, 374.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (211, 326, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (343.37, 164.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 417, 4675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13643) [M::mem_pestat] mean and std.dev: (2264.15, 2234.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18127) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 316, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (276.43, 95.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 684472 sequences (100000242 bp) in 2.192 CPU sec, 2.266 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 281659, 10, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 266, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (299.20, 182.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (338.72, 163.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 338, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2213) [M::mem_pestat] mean and std.dev: (556.22, 433.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 392, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2971) [M::mem_pestat] mean and std.dev: (652.45, 614.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683836 sequences (100000253 bp) in 1.800 CPU sec, 1.805 real sec... [M::mem_process_seqs] Processed 681598 reads in 152.646 CPU sec, 4.834 real sec [M::mem_process_seqs] Processed 682030 reads in 98.801 CPU sec, 3.116 real sec sorting block 111, #reads = 1075426 mem = 536881089 peak = 1151156224 [M::process] read 683050 sequences (100000143 bp) in 2.214 CPU sec, 2.313 real sec... [M::process] read 682872 sequences (100000104 bp) in 1.430 CPU sec, 1.428 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281967, 14, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 429, 1500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4038) [M::mem_pestat] mean and std.dev: (998.81, 1286.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 308, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (328.92, 160.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 1146, 2551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6925) [M::mem_pestat] mean and std.dev: (1549.00, 1192.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 258, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2270) [M::mem_pestat] mean and std.dev: (402.43, 406.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683836 reads in 86.930 CPU sec, 2.751 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282843, 9, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (208.38, 101.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 303, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (325.11, 159.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 251, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (245.60, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281896, 13, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 379, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (349.93, 137.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 314, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (334.00, 161.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1198, 1198, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (682, 1972) [M::mem_pestat] mean and std.dev: (1262.50, 111.72) [M::mem_pestat] low and high boundaries for proper pairs: (424, 2230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 268, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (304.29, 200.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684472 reads in 241.092 CPU sec, 7.652 real sec [M::process] read 682600 sequences (100000078 bp) in 1.861 CPU sec, 1.866 real sec... sorting block 112, #reads = 1077027 mem = 536879449 peak = 1151156224 [M::mem_process_seqs] Processed 683050 reads in 117.652 CPU sec, 3.743 real sec sorting block 113, #reads = 1077558 mem = 536879484 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282312, 5, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 405, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2171) [M::mem_pestat] mean and std.dev: (498.66, 380.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (204, 314, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (334.61, 162.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 317, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (311.79, 139.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681916 sequences (100000110 bp) in 2.364 CPU sec, 2.475 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281508, 14, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 154, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (167.19, 78.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 316, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (335.63, 162.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2407, 2407, 2485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2251, 2641) [M::mem_pestat] mean and std.dev: (2424.33, 32.43) [M::mem_pestat] low and high boundaries for proper pairs: (2173, 2719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 313, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (303.83, 137.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681892 sequences (100000042 bp) in 1.818 CPU sec, 1.822 real sec... [M::mem_process_seqs] Processed 682872 reads in 165.244 CPU sec, 5.248 real sec [M::mem_process_seqs] Processed 682600 reads in 116.719 CPU sec, 3.703 real sec sorting block 114, #reads = 1075101 mem = 536878613 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281189, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 316, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (328.87, 245.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 319, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (339.03, 163.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (239, 310, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3794) [M::mem_pestat] mean and std.dev: (567.56, 476.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4979) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 352, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657) [M::mem_pestat] mean and std.dev: (371.00, 261.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682060 sequences (100000168 bp) in 2.302 CPU sec, 2.429 real sec... [M::process] read 683014 sequences (100000197 bp) in 1.869 CPU sec, 1.862 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281714, 13, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 349, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614) [M::mem_pestat] mean and std.dev: (458.55, 359.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (339.28, 164.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (389, 1030, 1596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4010) [M::mem_pestat] mean and std.dev: (831.33, 605.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 324, 1173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2999) [M::mem_pestat] mean and std.dev: (482.83, 450.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681916 reads in 167.334 CPU sec, 5.307 real sec [M::mem_process_seqs] Processed 681892 reads in 110.431 CPU sec, 3.501 real sec sorting block 115, #reads = 1074813 mem = 536881993 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281486, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 238, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (300.76, 143.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 316, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (336.97, 163.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 296, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (257.15, 88.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682696 sequences (100000114 bp) in 2.462 CPU sec, 2.556 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281656, 6, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 307, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (324.19, 167.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (329.22, 159.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 299, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (287.45, 161.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682534 sequences (100000015 bp) in 1.870 CPU sec, 1.867 real sec... [M::mem_process_seqs] Processed 682060 reads in 166.963 CPU sec, 5.294 real sec sorting block 116, #reads = 1075067 mem = 536886182 peak = 1155170304 [M::mem_process_seqs] Processed 683014 reads in 112.413 CPU sec, 3.566 real sec sorting block 117, #reads = 1072165 mem = 536877519 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 281819, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (282.11, 125.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 311, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (334.43, 163.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 312, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (277.39, 104.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682248 sequences (100000195 bp) in 2.329 CPU sec, 2.438 real sec... [M::process] read 682798 sequences (100000055 bp) in 1.870 CPU sec, 1.868 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281252, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 231, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (232.83, 84.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 311, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (334.31, 163.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 291, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (273.31, 133.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682696 reads in 170.696 CPU sec, 5.414 real sec [M::mem_process_seqs] Processed 682534 reads in 108.881 CPU sec, 3.443 real sec sorting block 118, #reads = 1073824 mem = 536883142 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 281019, 12, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 288, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1241) [M::mem_pestat] mean and std.dev: (366.42, 236.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 314, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (336.96, 164.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 853, 2410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5922) [M::mem_pestat] mean and std.dev: (1258.42, 984.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 229, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (300.96, 198.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 682680 sequences (100000179 bp) in 2.275 CPU sec, 2.374 real sec... [M::process] read 682518 sequences (100000138 bp) in 1.620 CPU sec, 1.621 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281825, 5, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 271, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (324.86, 203.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 311, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (335.09, 165.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 239, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (247.31, 101.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682248 reads in 165.687 CPU sec, 5.271 real sec [M::mem_process_seqs] Processed 682798 reads in 113.910 CPU sec, 3.634 real sec sorting block 119, #reads = 1073611 mem = 536878727 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281418, 13, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 407, 1764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4942) [M::mem_pestat] mean and std.dev: (848.96, 740.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (202, 311, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (336.10, 166.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1698, 1859, 2446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (202, 3942) [M::mem_pestat] mean and std.dev: (1923.31, 623.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4690) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 333, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (354.23, 205.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 683048 sequences (100000060 bp) in 2.390 CPU sec, 2.478 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 281423, 7, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 261, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (288.49, 158.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 311, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (335.64, 166.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 281, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (329.00, 199.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682602 sequences (100000060 bp) in 1.930 CPU sec, 1.922 real sec... [M::mem_process_seqs] Processed 682680 reads in 170.200 CPU sec, 5.399 real sec sorting block 120, #reads = 1073635 mem = 536877780 peak = 1155170304 [M::mem_process_seqs] Processed 682518 reads in 111.984 CPU sec, 3.538 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281795, 12, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 235, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370) [M::mem_pestat] mean and std.dev: (279.48, 238.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 306, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (330.48, 163.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (283, 677, 2484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6886) [M::mem_pestat] mean and std.dev: (978.67, 1066.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9087) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 292, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2928) [M::mem_pestat] mean and std.dev: (507.15, 513.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687524 sequences (100000140 bp) in 2.473 CPU sec, 2.554 real sec... [M::process] read 685756 sequences (100000230 bp) in 1.470 CPU sec, 1.468 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281504, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 323, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2104) [M::mem_pestat] mean and std.dev: (406.73, 355.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 308, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (331.70, 162.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (338, 510, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (448.60, 221.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 362, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (356.63, 198.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683048 reads in 182.074 CPU sec, 5.791 real sec sorting block 121, #reads = 1072571 mem = 536878961 peak = 1155170304 [M::mem_process_seqs] Processed 682602 reads in 121.318 CPU sec, 3.872 real sec sorting block 122, #reads = 1068704 mem = 536879224 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282925, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 316, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260) [M::mem_pestat] mean and std.dev: (343.90, 246.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 284, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (312.62, 157.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 257, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361) [M::mem_pestat] mean and std.dev: (281.97, 190.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 684496 sequences (100000251 bp) in 2.400 CPU sec, 2.465 real sec... [M::process] read 683700 sequences (100000260 bp) in 1.850 CPU sec, 1.854 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283274, 7, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 279, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (278.28, 124.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (320.60, 159.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 261, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (319.14, 205.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687524 reads in 177.449 CPU sec, 5.631 real sec [M::mem_process_seqs] Processed 685756 reads in 112.827 CPU sec, 3.579 real sec sorting block 123, #reads = 1067389 mem = 536876267 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283130, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 324, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3384) [M::mem_pestat] mean and std.dev: (562.18, 561.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 304, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (327.12, 160.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 227, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (217.65, 93.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 683100 sequences (100000031 bp) in 2.687 CPU sec, 2.759 real sec... [M::process] read 682596 sequences (100000205 bp) in 1.480 CPU sec, 1.479 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282437, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 325, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267) [M::mem_pestat] mean and std.dev: (480.18, 433.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 310, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (331.79, 161.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 305, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (318.38, 146.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 684496 reads in 168.009 CPU sec, 5.326 real sec [M::mem_process_seqs] Processed 683700 reads in 108.321 CPU sec, 3.440 real sec sorting block 124, #reads = 1072291 mem = 536877404 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282096, 18, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 386, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1755) [M::mem_pestat] mean and std.dev: (539.18, 474.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 314, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (333.33, 160.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (437, 640, 2377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6257) [M::mem_pestat] mean and std.dev: (1352.61, 1073.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 268, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (263.62, 94.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681992 sequences (100000170 bp) in 2.509 CPU sec, 2.588 real sec... [M::process] read 681308 sequences (100000274 bp) in 1.820 CPU sec, 1.819 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282015, 11, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 261, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (240.21, 83.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 317, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (335.81, 160.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 2189, 2890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8046) [M::mem_pestat] mean and std.dev: (2144.27, 1836.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 239, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (273.38, 230.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 683100 reads in 165.265 CPU sec, 5.232 real sec sorting block 125, #reads = 1072417 mem = 536881209 peak = 1155170304 [M::mem_process_seqs] Processed 682596 reads in 105.650 CPU sec, 3.342 real sec sorting block 126, #reads = 1072618 mem = 536879380 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281889, 7, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 327, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (324.58, 190.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 321, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (338.36, 160.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 268, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (238.92, 84.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 682768 sequences (100000004 bp) in 2.713 CPU sec, 2.796 real sec... [M::process] read 682866 sequences (100000122 bp) in 1.760 CPU sec, 1.755 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 281210, 10, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 230, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (221.45, 99.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (210, 326, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (342.34, 162.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (570, 3123, 3989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10827) [M::mem_pestat] mean and std.dev: (2575.40, 1781.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14246) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 229, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (292.83, 152.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681992 reads in 168.481 CPU sec, 5.336 real sec [M::mem_process_seqs] Processed 681308 reads in 107.439 CPU sec, 3.405 real sec sorting block 127, #reads = 1071972 mem = 536878664 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 281699, 15, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 290, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (320.16, 218.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (205, 317, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (335.57, 161.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (441, 552, 2737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7329) [M::mem_pestat] mean and std.dev: (1684.00, 1624.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 369, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1374) [M::mem_pestat] mean and std.dev: (465.17, 352.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681968 sequences (100000209 bp) in 2.509 CPU sec, 2.585 real sec... [M::process] read 681904 sequences (100000108 bp) in 1.420 CPU sec, 1.412 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 281866, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 231, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388) [M::mem_pestat] mean and std.dev: (352.00, 357.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (203, 311, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (331.21, 158.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 258, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (367.35, 323.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 682768 reads in 166.277 CPU sec, 5.281 real sec [M::mem_process_seqs] Processed 682866 reads in 107.755 CPU sec, 3.425 real sec sorting block 128, #reads = 1073467 mem = 536879652 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 281813, 8, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 330, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1350) [M::mem_pestat] mean and std.dev: (371.59, 242.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 316, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (334.28, 158.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 247, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (352.32, 257.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681568 sequences (100000087 bp) in 2.333 CPU sec, 2.417 real sec... [M::process] read 681958 sequences (100000196 bp) in 1.850 CPU sec, 1.847 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281070, 8, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 268, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (248.12, 125.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (335.26, 159.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 259, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1494) [M::mem_pestat] mean and std.dev: (331.85, 270.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681968 reads in 163.606 CPU sec, 5.402 real sec sorting block 129, #reads = 1073922 mem = 536891154 peak = 1155170304 [M::mem_process_seqs] Processed 681904 reads in 105.998 CPU sec, 3.357 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280810, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 262, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (259.75, 120.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 318, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (336.10, 158.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 306, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (293.57, 160.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1433) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681724 sequences (100000253 bp) in 2.629 CPU sec, 2.695 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281799, 6, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 296, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (269.82, 127.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 314, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (330.75, 154.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 278, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (275.14, 163.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681360 sequences (100000091 bp) in 1.935 CPU sec, 1.932 real sec... [M::mem_process_seqs] Processed 681568 reads in 168.080 CPU sec, 5.318 real sec sorting block 130, #reads = 1073286 mem = 536877059 peak = 1155170304 [M::mem_process_seqs] Processed 681958 reads in 111.358 CPU sec, 3.513 real sec sorting block 131, #reads = 1071391 mem = 536879831 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 281287, 9, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 362, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1249) [M::mem_pestat] mean and std.dev: (374.72, 212.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 312, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (330.14, 154.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 240, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (365.79, 212.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681324 sequences (100000057 bp) in 2.607 CPU sec, 2.672 real sec... [M::process] read 681204 sequences (100000266 bp) in 1.720 CPU sec, 1.718 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280778, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 284, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (247.21, 121.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 313, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (330.81, 155.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 297, 774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1848) [M::mem_pestat] mean and std.dev: (430.30, 252.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681724 reads in 164.703 CPU sec, 5.213 real sec [M::mem_process_seqs] Processed 681360 reads in 106.666 CPU sec, 3.381 real sec sorting block 132, #reads = 1071717 mem = 536876857 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281269, 9, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 273, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1377) [M::mem_pestat] mean and std.dev: (477.35, 348.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (330.50, 155.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 271, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (262.02, 112.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681350 sequences (100000100 bp) in 2.423 CPU sec, 2.494 real sec... [M::process] read 681464 sequences (100000106 bp) in 1.480 CPU sec, 1.476 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280831, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 303, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (337.23, 189.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 312, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (330.80, 155.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 469, 1680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4636) [M::mem_pestat] mean and std.dev: (788.07, 893.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681324 reads in 169.981 CPU sec, 5.628 real sec [M::mem_process_seqs] Processed 681204 reads in 106.317 CPU sec, 3.380 real sec sorting block 133, #reads = 1072446 mem = 536879775 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280626, 9, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 366, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (435.46, 319.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 310, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (328.50, 153.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 200, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (215.76, 104.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681640 sequences (100000183 bp) in 2.345 CPU sec, 2.423 real sec... [M::process] read 681358 sequences (100000165 bp) in 1.700 CPU sec, 1.699 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280771, 9, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 224, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224) [M::mem_pestat] mean and std.dev: (320.82, 252.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (207, 309, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (329.63, 155.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 281, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (287.03, 138.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681350 reads in 159.812 CPU sec, 5.066 real sec sorting block 134, #reads = 1073776 mem = 536884275 peak = 1155170304 [M::mem_process_seqs] Processed 681464 reads in 102.328 CPU sec, 3.239 real sec sorting block 135, #reads = 1074498 mem = 536880373 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 280788, 10, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 240, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (292.96, 211.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (206, 309, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (327.94, 154.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (519, 4548, 5478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15396) [M::mem_pestat] mean and std.dev: (4423.80, 3158.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 234, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (254.09, 107.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 680890 sequences (100000142 bp) in 2.658 CPU sec, 2.716 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281477, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 265, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (278.81, 153.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 311, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (330.39, 155.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 297, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (290.14, 167.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 680928 sequences (100000260 bp) in 1.817 CPU sec, 1.817 real sec... [M::mem_process_seqs] Processed 681640 reads in 159.422 CPU sec, 5.045 real sec [M::mem_process_seqs] Processed 681358 reads in 102.083 CPU sec, 3.217 real sec sorting block 136, #reads = 1074743 mem = 536879113 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280268, 18, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 274, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3105) [M::mem_pestat] mean and std.dev: (648.47, 659.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 312, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (330.19, 154.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (827, 2466, 2466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5744) [M::mem_pestat] mean and std.dev: (2065.28, 1394.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 378, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2605) [M::mem_pestat] mean and std.dev: (597.11, 568.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 681076 sequences (100000240 bp) in 2.527 CPU sec, 2.602 real sec... [M::process] read 681040 sequences (100000017 bp) in 1.490 CPU sec, 1.482 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 281170, 4, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 282, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (224.77, 85.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 312, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (331.76, 156.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 279, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (241.06, 97.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 680890 reads in 158.954 CPU sec, 5.051 real sec [M::mem_process_seqs] Processed 680928 reads in 102.468 CPU sec, 3.250 real sec sorting block 137, #reads = 1074412 mem = 536879249 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 281113, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 291, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (285.70, 124.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 313, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (333.07, 157.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 297, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (325.81, 234.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 681380 sequences (100000011 bp) in 2.442 CPU sec, 2.532 real sec... [M::process] read 44706 sequences (6544807 bp) in 0.380 CPU sec, 0.371 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 281057, 1, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 284, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839) [M::mem_pestat] mean and std.dev: (402.86, 279.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (209, 314, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (333.87, 158.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 326, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785) [M::mem_pestat] mean and std.dev: (355.40, 243.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 681076 reads in 153.597 CPU sec, 4.914 real sec [M::mem_process_seqs] Processed 681040 reads in 97.732 CPU sec, 3.135 real sec sorting block 138, #reads = 1075158 mem = 536880843 peak = 1155170304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 281037, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 325, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1722) [M::mem_pestat] mean and std.dev: (457.53, 324.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (208, 313, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (334.30, 160.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 251, 2234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6464) [M::mem_pestat] mean and std.dev: (621.56, 865.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 276, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (280.94, 119.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 18426, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 304, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (324.38, 155.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 681380 reads in 94.435 CPU sec, 3.062 real sec [M::mem_process_seqs] Processed 44706 reads in 22.994 CPU sec, 0.747 real sec sorting block 139, #reads = 1075977 mem = 536880288 peak = 1155170304 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 150081070 overall: 70060371968 mem 21790.759 user 583.368 sys 737.573 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_4XR-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:1136_4XR-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_4XR-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_4XR-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_4XR-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 737.628 sec; CPU: 22374.130 sec sorting block 140, #reads = 549895 mem = 275679584 peak = 1155170304 uniq header count 1 merging 141 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 8043024701 output reads: 150984734 bam_mem_sort: 141 calls 79.886 user 1.611 sys 82.035 real bam_write: 141 calls 45.716 user 2.732 sys 58.657 real execute: 1 calls 43.079 user 1.186 sys 787.763 real merge_files: 1 calls 43.058 user 1.154 sys 49.847 real parse_chunk: 2249 calls 92.654 user 25.260 sys 122.848 real read_chunk: 6066 calls 49.568 user 40.242 sys 675.485 real sort_block: 1 calls 33.862 user 39.479 sys 652.724 real write_chunk: 3815 calls 42.690 user 0.870 sys 48.889 real overall: 3297017856 mem 1975.582 user 91.694 sys 787.796 real ls: cannot access '*.cram': No such file or directory