cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_87O-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2527.386 CPU sec, 111.903 real sec [M::process] read 697924 sequences (100000071 bp) in 1.225 CPU sec, 1.227 real sec... [M::process] read 697562 sequences (100000216 bp) in 1.675 CPU sec, 1.705 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285760, 1, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 178, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 363) [M::mem_pestat] mean and std.dev: (185.97, 62.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (278.41, 142.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 299, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (251.33, 113.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697548 sequences (100000210 bp) in 2.086 CPU sec, 2.104 real sec... [M::process] read 696846 sequences (100000063 bp) in 1.673 CPU sec, 1.782 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285685, 3, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 246, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (253.95, 115.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 255, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (280.97, 143.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 222, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (238.38, 76.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697924 reads in 211.825 CPU sec, 6.876 real sec [M::mem_process_seqs] Processed 697562 reads in 120.238 CPU sec, 4.037 real sec sorting block 0, #reads = 1085521 mem = 536877344 peak = 551292928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 285558, 1, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 263, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (267.82, 103.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (281.48, 143.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 211, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (226.67, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696038 sequences (100000238 bp) in 2.710 CPU sec, 3.085 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285587, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 270, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (312.41, 181.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (283.51, 144.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 293, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (262.71, 82.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695504 sequences (100000124 bp) in 1.751 CPU sec, 1.773 real sec... [M::mem_process_seqs] Processed 697548 reads in 178.223 CPU sec, 5.738 real sec [M::mem_process_seqs] Processed 696846 reads in 116.369 CPU sec, 3.748 real sec sorting block 1, #reads = 1085180 mem = 536879459 peak = 673976320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285339, 0, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 233, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (230.70, 84.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (285.61, 145.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 324, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1264) [M::mem_pestat] mean and std.dev: (419.30, 301.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694760 sequences (100000100 bp) in 2.947 CPU sec, 3.306 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285131, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 193, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (223.38, 84.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (287.98, 147.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 250, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (251.55, 78.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696038 reads in 181.360 CPU sec, 5.822 real sec [M::process] read 694548 sequences (100000072 bp) in 1.815 CPU sec, 1.878 real sec... [M::mem_process_seqs] Processed 695504 reads in 116.691 CPU sec, 3.736 real sec sorting block 2, #reads = 1085557 mem = 536878163 peak = 706936832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285154, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 202, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (236.52, 116.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (290.86, 148.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 234, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (243.76, 110.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694264 sequences (100000084 bp) in 3.371 CPU sec, 3.740 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284556, 3, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 202, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (228.05, 87.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (292.55, 149.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 294, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (253.80, 106.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694760 reads in 186.451 CPU sec, 6.020 real sec [M::mem_process_seqs] Processed 694548 reads in 118.711 CPU sec, 3.800 real sec sorting block 3, #reads = 1084453 mem = 536879709 peak = 706936832 [M::process] read 693872 sequences (100000256 bp) in 2.406 CPU sec, 2.502 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284898, 5, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 210, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (228.38, 119.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (294.24, 150.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 310, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (301.07, 135.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 4, #reads = 1083676 mem = 536879353 peak = 750444544 [M::process] read 693768 sequences (100000271 bp) in 3.092 CPU sec, 3.476 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284968, 4, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 252, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (250.39, 112.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (295.45, 151.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 219, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (202.45, 77.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694264 reads in 187.640 CPU sec, 6.141 real sec [M::mem_process_seqs] Processed 693872 reads in 120.544 CPU sec, 3.894 real sec [M::process] read 693826 sequences (100000036 bp) in 3.110 CPU sec, 3.445 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284044, 3, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 190, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (275.96, 173.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (296.68, 152.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 254, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (261.71, 107.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 5, #reads = 1084170 mem = 536879878 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284438, 5, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 202, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (312.45, 258.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (295.25, 150.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 291, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (292.20, 126.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693768 reads in 185.620 CPU sec, 6.088 real sec [M::process] read 693544 sequences (100000019 bp) in 3.387 CPU sec, 3.780 real sec... [M::mem_process_seqs] Processed 693826 reads in 125.278 CPU sec, 4.102 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284580, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 287, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (303.47, 127.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (294.92, 150.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 252, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 469) [M::mem_pestat] mean and std.dev: (244.92, 87.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693396 sequences (100000047 bp) in 3.306 CPU sec, 3.680 real sec... [M::mem_process_seqs] Processed 693544 reads in 99.176 CPU sec, 3.401 real sec sorting block 6, #reads = 1083007 mem = 536878582 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283746, 7, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 167, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (98, 258) [M::mem_pestat] mean and std.dev: (169.57, 30.30) [M::mem_pestat] low and high boundaries for proper pairs: (48, 291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (294.38, 149.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 196, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (257.59, 114.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693650 sequences (100000144 bp) in 3.114 CPU sec, 3.444 real sec... [M::mem_process_seqs] Processed 693396 reads in 109.029 CPU sec, 3.875 real sec sorting block 7, #reads = 1082788 mem = 536879378 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284025, 0, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 251, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (232.67, 113.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (294.41, 149.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 251, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (337.06, 239.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693650 reads in 104.879 CPU sec, 3.640 real sec [M::process] read 693498 sequences (100000101 bp) in 3.422 CPU sec, 3.716 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284311, 5, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 170, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (179.96, 68.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (293.37, 148.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 238, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (255.00, 119.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693498 reads in 106.734 CPU sec, 3.710 real sec [M::process] read 693570 sequences (100000272 bp) in 3.587 CPU sec, 3.939 real sec... sorting block 8, #reads = 1082432 mem = 536885479 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283816, 2, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 2019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5751) [M::mem_pestat] mean and std.dev: (665.96, 862.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.32, 149.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007) [M::mem_pestat] mean and std.dev: (296.19, 188.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693946 sequences (100000058 bp) in 2.974 CPU sec, 3.386 real sec... [M::mem_process_seqs] Processed 693570 reads in 104.386 CPU sec, 3.624 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284089, 5, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 260, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (335.32, 199.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 265, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (291.32, 147.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 219, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (224.23, 80.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 9, #reads = 1083496 mem = 536879255 peak = 750444544 [M::mem_process_seqs] Processed 693946 reads in 105.289 CPU sec, 3.608 real sec [M::process] read 693550 sequences (100000199 bp) in 3.871 CPU sec, 4.438 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284257, 2, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 199, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (196.07, 76.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.54, 148.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 323, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (282.82, 127.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693318 sequences (100000265 bp) in 3.126 CPU sec, 3.515 real sec... [M::mem_process_seqs] Processed 693550 reads in 104.880 CPU sec, 3.537 real sec sorting block 10, #reads = 1082339 mem = 536878997 peak = 750444544 [M::process] read 692856 sequences (100000087 bp) in 2.572 CPU sec, 2.830 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284080, 2, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (175.92, 79.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (294.27, 149.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 226, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (195.55, 53.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693318 reads in 103.478 CPU sec, 3.520 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283301, 5, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 234, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (244.34, 122.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (296.26, 150.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 334, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (341.68, 134.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 11, #reads = 1083037 mem = 536879381 peak = 750444544 [M::mem_process_seqs] Processed 692856 reads in 103.007 CPU sec, 3.504 real sec [M::process] read 692702 sequences (100000074 bp) in 3.600 CPU sec, 4.124 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283167, 4, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 247, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (302.95, 157.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (295.24, 149.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 253, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (263.02, 129.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692534 sequences (100000223 bp) in 2.779 CPU sec, 3.045 real sec... [M::mem_process_seqs] Processed 692702 reads in 102.957 CPU sec, 3.620 real sec sorting block 12, #reads = 1083121 mem = 536878904 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283959, 2, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 250, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (259.73, 85.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (296.31, 149.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 210, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (254.15, 126.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691542 sequences (100000039 bp) in 2.862 CPU sec, 3.121 real sec... [M::mem_process_seqs] Processed 692534 reads in 97.171 CPU sec, 3.295 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283517, 2, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (228.44, 110.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 275, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (298.55, 149.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 286, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (307.82, 146.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691542 reads in 100.861 CPU sec, 3.418 real sec sorting block 13, #reads = 1081754 mem = 536883078 peak = 750444544 [M::process] read 692040 sequences (100000170 bp) in 3.207 CPU sec, 3.546 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283682, 4, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 223, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (199.11, 77.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 273, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (297.44, 148.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 224, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (215.90, 73.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691234 sequences (100000115 bp) in 2.818 CPU sec, 3.081 real sec... [M::mem_process_seqs] Processed 692040 reads in 98.717 CPU sec, 3.325 real sec sorting block 14, #reads = 1082890 mem = 536877096 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283751, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 309, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (268.75, 105.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 277, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (300.03, 149.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 242, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (217.48, 75.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690994 sequences (100000295 bp) in 2.893 CPU sec, 3.179 real sec... [M::mem_process_seqs] Processed 691234 reads in 99.308 CPU sec, 3.336 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283299, 7, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 216, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (225.83, 104.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 279, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (301.79, 150.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 208, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (242.72, 128.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690994 reads in 98.993 CPU sec, 3.515 real sec sorting block 15, #reads = 1082029 mem = 536887502 peak = 750444544 [M::process] read 690882 sequences (100000253 bp) in 3.222 CPU sec, 3.563 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283432, 11, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 321, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (343.22, 178.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (303.53, 152.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 401, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (340.00, 293.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1632) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 232, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 412) [M::mem_pestat] mean and std.dev: (209.21, 52.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690882 reads in 101.084 CPU sec, 3.495 real sec [M::process] read 690816 sequences (100000009 bp) in 3.311 CPU sec, 3.624 real sec... sorting block 16, #reads = 1080453 mem = 536879101 peak = 750444544 [M::process] read 689854 sequences (100000040 bp) in 2.388 CPU sec, 2.632 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283479, 9, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 272, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (276.25, 137.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (304.04, 152.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 395, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (293.29, 135.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690816 reads in 104.618 CPU sec, 3.619 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283361, 6, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 268, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (244.81, 81.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 285, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (307.05, 152.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 216, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (217.42, 105.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694338 sequences (100000289 bp) in 2.763 CPU sec, 2.947 real sec... [M::mem_process_seqs] Processed 689854 reads in 105.523 CPU sec, 3.631 real sec sorting block 17, #reads = 1081508 mem = 536880455 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284621, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 191, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197) [M::mem_pestat] mean and std.dev: (290.17, 188.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.53, 148.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 166, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (168.61, 31.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 698048 sequences (100000135 bp) in 2.951 CPU sec, 3.211 real sec... [M::mem_process_seqs] Processed 694338 reads in 102.158 CPU sec, 3.462 real sec sorting block 18, #reads = 1081685 mem = 536880021 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 286184, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 185, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (229.04, 108.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (281.64, 145.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 184, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (205.76, 70.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697342 sequences (100000157 bp) in 2.824 CPU sec, 3.179 real sec... [M::mem_process_seqs] Processed 698048 reads in 108.048 CPU sec, 3.754 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285324, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 154, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (184.20, 80.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (285.01, 147.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 203, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (222.42, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697308 sequences (100000136 bp) in 2.813 CPU sec, 3.058 real sec... [M::mem_process_seqs] Processed 697342 reads in 100.128 CPU sec, 3.460 real sec sorting block 19, #reads = 1081560 mem = 536878160 peak = 750444544 [M::process] read 696482 sequences (100000158 bp) in 2.704 CPU sec, 2.916 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 285515, 1, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 154, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (167.80, 59.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (284.04, 146.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 244, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (298.24, 201.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697308 reads in 108.921 CPU sec, 3.711 real sec sorting block 20, #reads = 1088065 mem = 536878927 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285304, 10, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 175, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (269.45, 177.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (287.91, 148.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1423, 2434, 2434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4456) [M::mem_pestat] mean and std.dev: (2113.60, 543.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5467) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 222, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (212.47, 74.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696348 sequences (100000121 bp) in 2.726 CPU sec, 2.913 real sec... [M::mem_process_seqs] Processed 696482 reads in 100.897 CPU sec, 3.543 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285434, 0, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (253.96, 107.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 262, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (288.28, 148.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 255, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (85, 395) [M::mem_pestat] mean and std.dev: (228.89, 33.07) [M::mem_pestat] low and high boundaries for proper pairs: (23, 457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696140 sequences (100000240 bp) in 2.766 CPU sec, 2.998 real sec... [M::mem_process_seqs] Processed 696348 reads in 96.853 CPU sec, 3.238 real sec sorting block 21, #reads = 1086704 mem = 536879297 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284871, 1, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 190, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (203.16, 81.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 262, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (288.87, 148.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 201, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (211.15, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695440 sequences (100000051 bp) in 2.889 CPU sec, 3.121 real sec... [M::mem_process_seqs] Processed 696140 reads in 106.690 CPU sec, 3.738 real sec [M::process] read 694826 sequences (100000227 bp) in 2.845 CPU sec, 3.105 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284686, 2, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 181, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (171.60, 59.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 265, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (291.53, 149.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 241, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (260.33, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 22, #reads = 1087084 mem = 536880966 peak = 750444544 [M::mem_process_seqs] Processed 695440 reads in 113.921 CPU sec, 3.916 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284453, 0, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 257, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (250.97, 125.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (292.91, 149.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 477, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (317.12, 144.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694470 sequences (100000297 bp) in 2.838 CPU sec, 3.104 real sec... [M::mem_process_seqs] Processed 694826 reads in 101.068 CPU sec, 3.475 real sec sorting block 23, #reads = 1085761 mem = 536878584 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284627, 5, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 262, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (253.52, 71.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (295.36, 151.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 278, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3586) [M::mem_pestat] mean and std.dev: (683.40, 821.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694470 reads in 99.599 CPU sec, 3.353 real sec [M::process] read 694074 sequences (100000052 bp) in 3.339 CPU sec, 3.771 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 284378, 1, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 254, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (234.45, 73.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 271, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (297.28, 152.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 241, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (241.26, 78.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694074 reads in 101.777 CPU sec, 3.448 real sec [M::process] read 694068 sequences (100000029 bp) in 3.891 CPU sec, 4.515 real sec... sorting block 24, #reads = 1085120 mem = 536879107 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284225, 6, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 267, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (263.32, 114.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (296.83, 152.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 273, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (244.03, 93.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694068 reads in 102.669 CPU sec, 3.463 real sec [M::process] read 694228 sequences (100000033 bp) in 3.685 CPU sec, 4.039 real sec... sorting block 25, #reads = 1085338 mem = 536878458 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284927, 5, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 222, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (234.84, 79.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (297.37, 153.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 377, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (342.79, 194.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694228 reads in 96.279 CPU sec, 3.218 real sec [M::process] read 693958 sequences (100000005 bp) in 3.204 CPU sec, 3.541 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284094, 2, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 250, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (252.68, 87.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 271, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (297.23, 152.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 481, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (372.67, 205.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693958 reads in 92.058 CPU sec, 3.074 real sec sorting block 26, #reads = 1084346 mem = 536880558 peak = 750444544 [M::process] read 694200 sequences (100000234 bp) in 3.580 CPU sec, 3.928 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284478, 0, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 226, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (224.77, 92.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (296.19, 152.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 169, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (185.44, 69.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694200 reads in 90.102 CPU sec, 3.003 real sec [M::process] read 694038 sequences (100000183 bp) in 4.094 CPU sec, 4.492 real sec... sorting block 27, #reads = 1083946 mem = 536878754 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284531, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 316, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1879) [M::mem_pestat] mean and std.dev: (479.52, 341.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (294.57, 151.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 213, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (221.57, 86.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694038 reads in 90.169 CPU sec, 3.005 real sec [M::process] read 694068 sequences (100000179 bp) in 3.806 CPU sec, 4.427 real sec... sorting block 28, #reads = 1084062 mem = 536879068 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284349, 0, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (167.89, 50.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (292.69, 149.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 181, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (39, 354) [M::mem_pestat] mean and std.dev: (181.94, 42.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694068 reads in 95.732 CPU sec, 3.162 real sec [M::process] read 694022 sequences (100000016 bp) in 3.418 CPU sec, 3.963 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284248, 9, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 245, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (263.84, 137.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (289.79, 146.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 247, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (247.95, 104.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694022 reads in 94.066 CPU sec, 3.116 real sec sorting block 29, #reads = 1083387 mem = 536883143 peak = 750444544 [M::process] read 694046 sequences (100000033 bp) in 3.866 CPU sec, 4.413 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284481, 2, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 224, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (194.88, 93.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (290.28, 147.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 189, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (226.19, 118.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694046 reads in 93.834 CPU sec, 3.078 real sec [M::process] read 693870 sequences (100000069 bp) in 3.769 CPU sec, 4.189 real sec... sorting block 30, #reads = 1083181 mem = 536881393 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284686, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 235, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1407) [M::mem_pestat] mean and std.dev: (358.44, 236.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (291.54, 147.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 240, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (285.95, 183.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693870 reads in 95.452 CPU sec, 3.133 real sec [M::process] read 693812 sequences (100000015 bp) in 3.784 CPU sec, 4.271 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283876, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 210, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (235.14, 127.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (293.03, 148.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 223, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (242.33, 105.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693812 reads in 96.952 CPU sec, 3.185 real sec sorting block 31, #reads = 1083662 mem = 536880199 peak = 750444544 [M::process] read 693314 sequences (100000084 bp) in 3.671 CPU sec, 4.071 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284084, 0, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 231, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (293.34, 202.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (293.77, 148.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 222, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1777) [M::mem_pestat] mean and std.dev: (423.67, 400.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693314 reads in 99.547 CPU sec, 3.243 real sec [M::process] read 692792 sequences (100000193 bp) in 3.274 CPU sec, 3.613 real sec... sorting block 32, #reads = 1082918 mem = 536878161 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284048, 13, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 180, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (195.94, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 268, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (292.66, 146.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (249, 739, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1719) [M::mem_pestat] mean and std.dev: (516.27, 243.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 330, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1548) [M::mem_pestat] mean and std.dev: (343.67, 152.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692792 reads in 96.490 CPU sec, 3.304 real sec [M::process] read 693108 sequences (100000272 bp) in 3.230 CPU sec, 3.600 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283897, 10, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 242, 1147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3165) [M::mem_pestat] mean and std.dev: (715.74, 860.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (293.96, 148.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (477, 577, 841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569) [M::mem_pestat] mean and std.dev: (562.12, 172.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (223.61, 84.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693108 reads in 96.086 CPU sec, 3.177 real sec [M::process] read 692624 sequences (100000286 bp) in 3.830 CPU sec, 4.232 real sec... sorting block 33, #reads = 1081907 mem = 536879356 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284388, 8, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (294.37, 203.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (296.47, 149.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 216, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (256.83, 149.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692624 reads in 94.745 CPU sec, 3.115 real sec [M::process] read 692324 sequences (100000220 bp) in 4.306 CPU sec, 4.933 real sec... sorting block 34, #reads = 1081281 mem = 536883991 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284455, 4, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 253, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (211.92, 66.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (297.05, 149.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 244, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (237.96, 79.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692324 reads in 96.296 CPU sec, 3.148 real sec [M::process] read 692222 sequences (100000172 bp) in 3.769 CPU sec, 4.346 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284581, 3, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 343, 1926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5474) [M::mem_pestat] mean and std.dev: (807.04, 971.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (299.00, 151.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 284, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (273.32, 104.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692222 reads in 96.997 CPU sec, 3.156 real sec [M::process] read 691892 sequences (100000197 bp) in 3.222 CPU sec, 3.600 real sec... sorting block 35, #reads = 1081740 mem = 536880614 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283890, 2, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 167, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (172.05, 67.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 274, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (299.03, 150.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 232, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (247.22, 104.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691892 reads in 95.632 CPU sec, 3.132 real sec [M::process] read 692016 sequences (100000159 bp) in 4.082 CPU sec, 4.643 real sec... sorting block 36, #reads = 1083200 mem = 536877516 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284174, 3, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 316, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (276.08, 129.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (300.02, 151.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 225, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (229.62, 81.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692016 reads in 96.053 CPU sec, 3.123 real sec [M::process] read 691890 sequences (100000166 bp) in 3.600 CPU sec, 4.107 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283688, 2, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 338, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (291.67, 101.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 277, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (302.34, 153.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (267.71, 150.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691890 reads in 99.937 CPU sec, 3.216 real sec sorting block 37, #reads = 1082348 mem = 536877872 peak = 750444544 [M::process] read 691298 sequences (100000184 bp) in 3.238 CPU sec, 3.632 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283129, 5, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 225, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 482) [M::mem_pestat] mean and std.dev: (225.68, 49.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 279, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (304.28, 154.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 289, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (344.34, 203.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691298 reads in 98.908 CPU sec, 3.192 real sec [M::process] read 699934 sequences (100000146 bp) in 3.821 CPU sec, 4.198 real sec... sorting block 38, #reads = 1080776 mem = 536878882 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 286003, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 376, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (331.81, 179.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 249, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (276.40, 142.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 211, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (75, 360) [M::mem_pestat] mean and std.dev: (204.03, 45.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699934 reads in 100.081 CPU sec, 3.232 real sec [M::process] read 698500 sequences (100000106 bp) in 3.071 CPU sec, 3.379 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 286248, 4, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 221, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (199.27, 100.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (280.25, 143.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 243, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (249.11, 101.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698500 reads in 99.050 CPU sec, 3.231 real sec [M::process] read 698628 sequences (100000277 bp) in 3.757 CPU sec, 4.088 real sec... sorting block 39, #reads = 1085020 mem = 536877180 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285816, 8, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 221, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (205.12, 96.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (280.64, 143.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 216, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (246.77, 129.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698628 reads in 100.663 CPU sec, 3.292 real sec [M::process] read 698362 sequences (100000016 bp) in 3.898 CPU sec, 4.287 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285248, 7, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (213.89, 67.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (281.13, 144.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 243, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170) [M::mem_pestat] mean and std.dev: (290.39, 172.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698362 reads in 98.381 CPU sec, 3.212 real sec sorting block 40, #reads = 1087168 mem = 536879494 peak = 750444544 [M::process] read 697180 sequences (100000151 bp) in 3.798 CPU sec, 4.184 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 285492, 1, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 246, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (229.00, 96.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (283.57, 144.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 211, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (254.07, 114.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697180 reads in 98.920 CPU sec, 3.216 real sec [M::process] read 696490 sequences (100000176 bp) in 3.673 CPU sec, 4.135 real sec... sorting block 41, #reads = 1085141 mem = 536879089 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284860, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 261, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (279.82, 158.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (285.51, 144.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (255.61, 96.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696490 reads in 99.092 CPU sec, 3.225 real sec [M::process] read 695686 sequences (100000067 bp) in 3.908 CPU sec, 4.386 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285257, 3, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 223, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (197.92, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (287.21, 145.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 322, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (315.05, 172.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695686 reads in 97.922 CPU sec, 3.187 real sec sorting block 42, #reads = 1084503 mem = 536878487 peak = 750444544 [M::process] read 695294 sequences (100000238 bp) in 3.924 CPU sec, 4.418 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284643, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 261, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (256.27, 116.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (289.40, 146.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 177, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (228.60, 109.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695294 reads in 99.528 CPU sec, 3.218 real sec [M::process] read 694936 sequences (100000043 bp) in 3.357 CPU sec, 3.780 real sec... sorting block 43, #reads = 1084749 mem = 536882371 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284562, 1, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 244, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2007) [M::mem_pestat] mean and std.dev: (521.52, 562.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (289.40, 145.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 193, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (225.00, 126.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694562 sequences (100000053 bp) in 2.814 CPU sec, 3.115 real sec... [M::mem_process_seqs] Processed 694936 reads in 101.177 CPU sec, 3.293 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284970, 4, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 175, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (194.50, 74.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (292.11, 148.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (232.20, 91.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694562 reads in 97.935 CPU sec, 3.225 real sec [M::process] read 693974 sequences (100000054 bp) in 3.699 CPU sec, 4.118 real sec... sorting block 44, #reads = 1083952 mem = 536879161 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284437, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 209, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (208.83, 84.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (293.67, 148.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 237, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (251.78, 103.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693974 reads in 96.974 CPU sec, 3.191 real sec [M::process] read 694190 sequences (100000133 bp) in 3.603 CPU sec, 4.028 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284270, 0, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 190, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (199.00, 60.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.21, 148.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (261.38, 134.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 45, #reads = 1083985 mem = 536878026 peak = 750444544 [M::mem_process_seqs] Processed 694190 reads in 99.148 CPU sec, 3.262 real sec [M::process] read 694162 sequences (100000017 bp) in 3.281 CPU sec, 3.705 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284623, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (231.22, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (294.42, 149.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 266, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (261.43, 110.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694162 reads in 98.528 CPU sec, 3.250 real sec [M::process] read 693354 sequences (100000183 bp) in 3.548 CPU sec, 4.042 real sec... sorting block 46, #reads = 1082885 mem = 536880529 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284107, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 179, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (205.27, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (296.11, 149.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 251, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (213.17, 73.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693354 reads in 96.057 CPU sec, 3.166 real sec [M::process] read 693780 sequences (100000025 bp) in 3.985 CPU sec, 4.391 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284350, 2, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 202, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (81, 306) [M::mem_pestat] mean and std.dev: (181.37, 44.33) [M::mem_pestat] low and high boundaries for proper pairs: (4, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (294.51, 148.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 232, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (272.03, 111.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693780 reads in 97.533 CPU sec, 3.191 real sec sorting block 47, #reads = 1083389 mem = 536885566 peak = 750444544 [M::process] read 694300 sequences (100000219 bp) in 3.635 CPU sec, 4.008 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284372, 0, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 260, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1386) [M::mem_pestat] mean and std.dev: (382.82, 262.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (290.74, 147.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 215, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 406) [M::mem_pestat] mean and std.dev: (219.93, 60.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694300 reads in 99.296 CPU sec, 3.241 real sec [M::process] read 693702 sequences (100000183 bp) in 3.263 CPU sec, 3.530 real sec... sorting block 48, #reads = 1083992 mem = 536878618 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283842, 10, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 259, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (270.29, 118.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (292.59, 148.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 77, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (104.00, 38.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 219, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (222.87, 74.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693526 sequences (100000026 bp) in 2.719 CPU sec, 2.934 real sec... [M::mem_process_seqs] Processed 693702 reads in 96.225 CPU sec, 3.109 real sec sorting block 49, #reads = 1083285 mem = 536881887 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284121, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (213.03, 75.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (291.97, 147.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 286, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1119) [M::mem_pestat] mean and std.dev: (316.62, 186.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693722 sequences (100000207 bp) in 2.715 CPU sec, 2.899 real sec... [M::mem_process_seqs] Processed 693526 reads in 95.279 CPU sec, 3.065 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284378, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 188, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (243.55, 113.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (291.99, 146.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 239, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (231.91, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693700 sequences (100000218 bp) in 2.802 CPU sec, 2.981 real sec... [M::mem_process_seqs] Processed 693722 reads in 93.805 CPU sec, 3.018 real sec sorting block 50, #reads = 1082410 mem = 536878991 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284379, 5, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 179, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (216.47, 112.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 267, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (292.40, 147.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 258, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (263.45, 108.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692874 sequences (100000157 bp) in 3.116 CPU sec, 3.334 real sec... [M::mem_process_seqs] Processed 693700 reads in 96.340 CPU sec, 3.093 real sec sorting block 51, #reads = 1083815 mem = 536878623 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284133, 0, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 200, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (186.71, 72.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (294.53, 147.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 249, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (315.97, 168.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692998 sequences (100000279 bp) in 3.004 CPU sec, 3.226 real sec... [M::mem_process_seqs] Processed 692874 reads in 99.703 CPU sec, 3.219 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283971, 4, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 246, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (262.69, 126.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (292.63, 146.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 208, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (229.16, 102.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692998 reads in 99.749 CPU sec, 3.204 real sec [M::process] read 692916 sequences (100000158 bp) in 3.453 CPU sec, 3.672 real sec... sorting block 52, #reads = 1081820 mem = 536877632 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284114, 12, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 226, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (211.64, 75.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (292.90, 146.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 573, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (385.08, 193.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1740) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 692916 reads in 99.002 CPU sec, 3.187 real sec [M::process] read 692804 sequences (100000157 bp) in 3.083 CPU sec, 3.384 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284167, 2, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 253, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4007) [M::mem_pestat] mean and std.dev: (745.18, 978.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (295.02, 148.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 240, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (281.12, 138.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692804 reads in 97.681 CPU sec, 3.154 real sec sorting block 53, #reads = 1081327 mem = 536877891 peak = 750444544 [M::process] read 692782 sequences (100000236 bp) in 3.427 CPU sec, 3.936 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284402, 6, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 205, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032) [M::mem_pestat] mean and std.dev: (278.20, 222.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (297.14, 150.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 196, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (189.68, 61.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692782 reads in 98.758 CPU sec, 3.200 real sec [M::process] read 692620 sequences (100000091 bp) in 2.871 CPU sec, 3.229 real sec... sorting block 54, #reads = 1082339 mem = 536878444 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 283877, 1, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 326, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (268.23, 104.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (296.68, 150.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 366, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322) [M::mem_pestat] mean and std.dev: (365.03, 266.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692712 sequences (100000228 bp) in 2.882 CPU sec, 3.089 real sec... [M::mem_process_seqs] Processed 692620 reads in 100.252 CPU sec, 3.233 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283908, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 282, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381) [M::mem_pestat] mean and std.dev: (298.47, 160.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (297.00, 150.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 283, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (331.06, 165.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692712 reads in 98.922 CPU sec, 3.187 real sec sorting block 55, #reads = 1080897 mem = 536880166 peak = 750444544 [M::process] read 692872 sequences (100000272 bp) in 3.457 CPU sec, 3.757 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284199, 1, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 210, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (229.67, 100.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (297.58, 151.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 156, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348) [M::mem_pestat] mean and std.dev: (316.75, 250.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692872 reads in 99.635 CPU sec, 3.221 real sec [M::process] read 692248 sequences (100000100 bp) in 3.007 CPU sec, 3.351 real sec... sorting block 56, #reads = 1082002 mem = 536877390 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283911, 10, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 236, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (246.33, 98.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (299.25, 152.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1421, 4026, 5883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14807) [M::mem_pestat] mean and std.dev: (3037.30, 2255.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 215, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (243.59, 110.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692248 reads in 99.328 CPU sec, 3.203 real sec [M::process] read 692434 sequences (100000178 bp) in 3.199 CPU sec, 3.443 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283870, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1307) [M::mem_pestat] mean and std.dev: (328.46, 269.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 272, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (297.19, 149.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 380, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136) [M::mem_pestat] mean and std.dev: (335.06, 193.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 57, #reads = 1080694 mem = 536880450 peak = 750444544 [M::process] read 698534 sequences (100000174 bp) in 2.607 CPU sec, 2.902 real sec... [M::mem_process_seqs] Processed 692434 reads in 100.906 CPU sec, 3.249 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 286079, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 136, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.71, 56.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (278.96, 142.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 317, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (273.27, 89.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698534 reads in 97.836 CPU sec, 3.146 real sec [M::process] read 697844 sequences (100000069 bp) in 3.724 CPU sec, 3.994 real sec... sorting block 58, #reads = 1082060 mem = 536879652 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285237, 1, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 280, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1953) [M::mem_pestat] mean and std.dev: (292.95, 214.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (281.89, 144.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 246, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (273.34, 122.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697512 sequences (100000095 bp) in 2.495 CPU sec, 2.748 real sec... [M::process] read 697180 sequences (100000172 bp) in 1.997 CPU sec, 2.004 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285460, 3, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 265, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (265.32, 86.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (282.65, 144.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 186, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125) [M::mem_pestat] mean and std.dev: (269.79, 195.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697512 reads in 94.368 CPU sec, 2.958 real sec [M::process] read 696684 sequences (100000214 bp) in 2.233 CPU sec, 2.296 real sec... [M::mem_process_seqs] Processed 697844 reads in 241.861 CPU sec, 7.689 real sec sorting block 59, #reads = 1086595 mem = 536879513 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285049, 5, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (279.00, 137.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (284.25, 145.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 231, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (223.97, 81.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696552 sequences (100000026 bp) in 2.616 CPU sec, 2.837 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285865, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 148, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (195.04, 104.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 260, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (286.00, 146.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 262, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (220.00, 52.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697180 reads in 187.487 CPU sec, 5.935 real sec [M::mem_process_seqs] Processed 696684 reads in 112.182 CPU sec, 3.545 real sec sorting block 60, #reads = 1085901 mem = 536876992 peak = 750444544 [M::process] read 696162 sequences (100000145 bp) in 2.415 CPU sec, 2.565 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285195, 5, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 221, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138) [M::mem_pestat] mean and std.dev: (263.00, 225.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (286.04, 146.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 221, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (196.43, 56.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695482 sequences (100000103 bp) in 2.326 CPU sec, 2.421 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284875, 7, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 335, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (298.95, 135.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 263, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (288.23, 146.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 264, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452) [M::mem_pestat] mean and std.dev: (271.09, 145.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696552 reads in 162.674 CPU sec, 5.149 real sec sorting block 61, #reads = 1085705 mem = 536876788 peak = 750444544 [M::mem_process_seqs] Processed 696162 reads in 107.426 CPU sec, 3.376 real sec [M::process] read 694968 sequences (100000042 bp) in 2.098 CPU sec, 2.135 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284842, 1, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 296, 1420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3936) [M::mem_pestat] mean and std.dev: (562.59, 589.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 265, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (290.49, 148.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 224, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (243.42, 92.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 62, #reads = 1085232 mem = 536877329 peak = 750444544 [M::process] read 694950 sequences (100000028 bp) in 2.683 CPU sec, 2.867 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284114, 5, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 151, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (156.60, 57.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 267, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (291.87, 148.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 284, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (240.70, 78.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695482 reads in 163.335 CPU sec, 5.209 real sec [M::mem_process_seqs] Processed 694968 reads in 107.332 CPU sec, 3.384 real sec [M::process] read 694730 sequences (100000061 bp) in 2.418 CPU sec, 2.514 real sec... sorting block 63, #reads = 1085972 mem = 536881054 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284916, 0, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 168, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (169.56, 61.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 267, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (291.33, 147.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 257, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (250.33, 82.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694134 sequences (100000023 bp) in 2.599 CPU sec, 2.746 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284294, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 310, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (283.93, 110.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (292.28, 147.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 205, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (273.61, 127.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694950 reads in 167.532 CPU sec, 5.322 real sec [M::process] read 694116 sequences (100000067 bp) in 2.145 CPU sec, 2.197 real sec... [M::mem_process_seqs] Processed 694730 reads in 112.679 CPU sec, 3.551 real sec sorting block 64, #reads = 1084138 mem = 536886167 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284345, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 224, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (297.28, 205.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (294.64, 149.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 223, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (254.19, 121.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694352 sequences (100000135 bp) in 3.172 CPU sec, 3.416 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284185, 5, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 230, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (225.91, 84.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (293.80, 148.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 255, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (277.55, 134.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694134 reads in 171.248 CPU sec, 5.461 real sec [M::mem_process_seqs] Processed 694116 reads in 113.747 CPU sec, 3.588 real sec sorting block 65, #reads = 1083654 mem = 536880286 peak = 750444544 [M::process] read 694900 sequences (100000004 bp) in 3.282 CPU sec, 3.559 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284844, 3, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 353) [M::mem_pestat] mean and std.dev: (181.00, 53.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (294.45, 149.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 215, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (252.67, 143.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 66, #reads = 1083696 mem = 536878002 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284774, 0, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 183, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (247.90, 152.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (293.11, 150.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 278, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (258.18, 117.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696692 sequences (100000290 bp) in 2.821 CPU sec, 3.007 real sec... [M::mem_process_seqs] Processed 694352 reads in 167.035 CPU sec, 5.351 real sec [M::mem_process_seqs] Processed 694900 reads in 111.176 CPU sec, 3.512 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 285683, 2, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 157, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (201.23, 128.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 256, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (283.07, 145.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 345, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197) [M::mem_pestat] mean and std.dev: (350.27, 183.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694578 sequences (100000101 bp) in 3.154 CPU sec, 3.420 real sec... sorting block 67, #reads = 1083469 mem = 536878364 peak = 750444544 [M::mem_process_seqs] Processed 696692 reads in 92.824 CPU sec, 3.045 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284639, 8, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 220, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (251.23, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (289.32, 146.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 213, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (247.41, 100.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694456 sequences (100000045 bp) in 3.208 CPU sec, 3.487 real sec... [M::mem_process_seqs] Processed 694578 reads in 99.928 CPU sec, 3.254 real sec sorting block 68, #reads = 1084462 mem = 536878876 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284352, 0, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 162, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (270.19, 224.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 263, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (287.77, 145.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 209, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (210.81, 83.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694112 sequences (100000134 bp) in 2.774 CPU sec, 3.001 real sec... [M::mem_process_seqs] Processed 694456 reads in 101.362 CPU sec, 3.261 real sec sorting block 69, #reads = 1082564 mem = 536879928 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283900, 3, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 240, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (295.16, 167.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (288.98, 144.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 295, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (257.70, 83.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693430 sequences (100000073 bp) in 2.988 CPU sec, 3.248 real sec... [M::mem_process_seqs] Processed 694112 reads in 97.717 CPU sec, 3.166 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 283433, 2, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 240, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (217.39, 91.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 265, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (288.41, 143.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 222, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (216.00, 100.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693510 sequences (100000093 bp) in 3.275 CPU sec, 3.520 real sec... [M::mem_process_seqs] Processed 693430 reads in 97.521 CPU sec, 3.137 real sec sorting block 70, #reads = 1081555 mem = 536878072 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284517, 4, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 163, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (216.60, 109.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 266, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (289.43, 144.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 240, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (243.52, 81.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693760 sequences (100000067 bp) in 2.953 CPU sec, 3.148 real sec... [M::mem_process_seqs] Processed 693510 reads in 99.209 CPU sec, 3.185 real sec sorting block 71, #reads = 1082378 mem = 536878890 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284182, 12, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (230.03, 107.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 265, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (288.91, 144.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (430, 670, 2662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7126) [M::mem_pestat] mean and std.dev: (1843.42, 2381.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 172, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (214.58, 98.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693498 sequences (100000111 bp) in 2.878 CPU sec, 3.112 real sec... [M::mem_process_seqs] Processed 693760 reads in 100.744 CPU sec, 3.232 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283886, 1, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 228, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (260.11, 130.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 265, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (287.89, 142.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 309, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (312.61, 154.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693320 sequences (100000153 bp) in 2.906 CPU sec, 3.104 real sec... [M::mem_process_seqs] Processed 693498 reads in 99.757 CPU sec, 3.204 real sec sorting block 72, #reads = 1081921 mem = 536884143 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283671, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 172, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (281.68, 168.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 265, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (287.93, 142.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 310, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2570) [M::mem_pestat] mean and std.dev: (587.86, 650.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3370) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693072 sequences (100000143 bp) in 3.219 CPU sec, 3.555 real sec... [M::mem_process_seqs] Processed 693320 reads in 102.503 CPU sec, 3.295 real sec sorting block 73, #reads = 1081317 mem = 536877751 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283885, 0, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 226, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (201.73, 61.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 268, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (291.55, 145.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 242, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 470) [M::mem_pestat] mean and std.dev: (226.94, 65.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692870 sequences (100000009 bp) in 2.909 CPU sec, 3.105 real sec... [M::mem_process_seqs] Processed 693072 reads in 97.641 CPU sec, 3.148 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283652, 6, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 155, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (183.05, 66.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (292.79, 146.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (244.00, 151.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692730 sequences (100000277 bp) in 2.755 CPU sec, 2.967 real sec... [M::mem_process_seqs] Processed 692870 reads in 100.641 CPU sec, 3.229 real sec sorting block 74, #reads = 1081587 mem = 536881675 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284199, 0, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 284, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (97, 437) [M::mem_pestat] mean and std.dev: (248.97, 68.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (294.57, 148.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 271, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (238.19, 92.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692480 sequences (100000156 bp) in 2.957 CPU sec, 3.172 real sec... [M::mem_process_seqs] Processed 692730 reads in 97.958 CPU sec, 3.146 real sec sorting block 75, #reads = 1081795 mem = 536879522 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283618, 2, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 272, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (273.50, 161.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 270, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (295.23, 148.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 213, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (231.57, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692260 sequences (100000221 bp) in 2.974 CPU sec, 3.173 real sec... [M::mem_process_seqs] Processed 692480 reads in 100.390 CPU sec, 3.232 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283902, 3, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 280, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (302.58, 159.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (297.78, 150.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 224, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (300.02, 176.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691598 sequences (100000013 bp) in 3.058 CPU sec, 3.265 real sec... [M::mem_process_seqs] Processed 692260 reads in 100.841 CPU sec, 3.226 real sec sorting block 76, #reads = 1082202 mem = 536885470 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283404, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 157, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (150.15, 53.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 277, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (300.74, 150.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 212, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (288.50, 183.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 704562 sequences (100000255 bp) in 2.818 CPU sec, 2.986 real sec... [M::mem_process_seqs] Processed 691598 reads in 99.055 CPU sec, 3.172 real sec sorting block 77, #reads = 1080368 mem = 536878874 peak = 750444544 [M::process] read 702296 sequences (100000097 bp) in 3.058 CPU sec, 3.242 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 288806, 3, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 151, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (161.27, 55.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 230, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (260.49, 136.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 269, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (263.29, 88.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 700130 sequences (100000061 bp) in 2.041 CPU sec, 2.045 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 287217, 2, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 268, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (293.00, 148.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 238, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (268.79, 141.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 293, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (242.36, 115.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704562 reads in 191.534 CPU sec, 6.020 real sec [M::mem_process_seqs] Processed 702296 reads in 124.973 CPU sec, 3.924 real sec [M::process] read 698718 sequences (100000157 bp) in 2.022 CPU sec, 2.084 real sec... sorting block 78, #reads = 1085031 mem = 536877878 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 286662, 2, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 249, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1881) [M::mem_pestat] mean and std.dev: (429.71, 434.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (275.64, 144.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 234, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239) [M::mem_pestat] mean and std.dev: (323.31, 214.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697410 sequences (100000168 bp) in 3.079 CPU sec, 3.301 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 286626, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 162, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (164.61, 66.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (280.50, 147.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 247, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (259.21, 98.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700130 reads in 180.562 CPU sec, 5.731 real sec [M::process] read 696852 sequences (100000297 bp) in 2.155 CPU sec, 2.187 real sec... [M::mem_process_seqs] Processed 698718 reads in 117.051 CPU sec, 3.691 real sec sorting block 79, #reads = 1084693 mem = 536878892 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285989, 6, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 229, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (219.65, 59.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (284.20, 148.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 233, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (247.88, 136.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696504 sequences (100000231 bp) in 3.023 CPU sec, 3.197 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 285573, 2, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 288, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (282.97, 134.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (285.69, 149.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 203, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (231.79, 83.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697410 reads in 174.602 CPU sec, 5.522 real sec [M::process] read 696080 sequences (100000211 bp) in 2.057 CPU sec, 2.067 real sec... [M::mem_process_seqs] Processed 696852 reads in 115.685 CPU sec, 3.647 real sec sorting block 80, #reads = 1086291 mem = 536884618 peak = 750444544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286100, 1, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 202, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (232.97, 103.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (287.79, 149.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 185, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (245.59, 131.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695930 sequences (100000111 bp) in 3.130 CPU sec, 3.322 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285560, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 130, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (158.50, 63.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (289.58, 150.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (240.38, 108.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696504 reads in 176.994 CPU sec, 5.608 real sec sorting block 81, #reads = 1085011 mem = 536878820 peak = 750444544 [M::process] read 695878 sequences (100000034 bp) in 2.327 CPU sec, 2.360 real sec... [M::mem_process_seqs] Processed 696080 reads in 117.259 CPU sec, 3.693 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284866, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 176, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (190.72, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 82, #reads = 1083587 mem = 536879445 peak = 771190784 [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (290.66, 150.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 274, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (243.61, 119.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695618 sequences (100000001 bp) in 3.051 CPU sec, 3.220 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284890, 5, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 176, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (192.40, 83.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (290.27, 150.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 215, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (232.07, 118.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695930 reads in 180.453 CPU sec, 5.720 real sec [M::process] read 695318 sequences (100000230 bp) in 2.120 CPU sec, 2.124 real sec... [M::mem_process_seqs] Processed 695878 reads in 122.105 CPU sec, 3.849 real sec sorting block 83, #reads = 1083009 mem = 536877174 peak = 771190784 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285665, 2, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 211, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (208.51, 75.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (292.27, 151.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 272, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (287.79, 99.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694960 sequences (100000230 bp) in 3.268 CPU sec, 3.473 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 285228, 2, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 231, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (217.67, 68.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (291.35, 150.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 216, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 382) [M::mem_pestat] mean and std.dev: (202.86, 52.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695618 reads in 184.026 CPU sec, 5.828 real sec [M::process] read 694588 sequences (100000155 bp) in 2.338 CPU sec, 2.357 real sec... [M::mem_process_seqs] Processed 695318 reads in 122.291 CPU sec, 3.853 real sec sorting block 84, #reads = 1082724 mem = 536879453 peak = 771190784 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284526, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 235, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (242.89, 102.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (292.40, 150.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 244, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (257.12, 128.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694498 sequences (100000013 bp) in 3.145 CPU sec, 3.324 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 285251, 7, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 151, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (192.39, 83.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (292.59, 149.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 317, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (291.89, 149.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694460 sequences (100000203 bp) in 1.910 CPU sec, 1.913 real sec... [M::mem_process_seqs] Processed 694960 reads in 187.485 CPU sec, 5.949 real sec sorting block 85, #reads = 1081704 mem = 536877406 peak = 771190784 [M::mem_process_seqs] Processed 694588 reads in 124.227 CPU sec, 3.928 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284575, 4, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 197, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (198.06, 85.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.49, 150.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 244, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (198.86, 62.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 86, #reads = 1081663 mem = 536876905 peak = 771190784 [M::process] read 694152 sequences (100000259 bp) in 3.545 CPU sec, 3.768 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284737, 2, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 196, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 320) [M::mem_pestat] mean and std.dev: (163.42, 50.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (292.55, 149.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 227, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (262.52, 122.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694498 reads in 184.872 CPU sec, 5.858 real sec [M::mem_process_seqs] Processed 694460 reads in 122.681 CPU sec, 3.862 real sec [M::process] read 694354 sequences (100000288 bp) in 2.587 CPU sec, 2.671 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284582, 8, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 227, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (203.66, 68.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (292.06, 149.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 354, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (282.18, 126.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 87, #reads = 1080793 mem = 536887302 peak = 771190784 [M::process] read 693954 sequences (100000103 bp) in 3.637 CPU sec, 3.888 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284525, 5, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 226, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (254.36, 123.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (291.63, 148.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 205, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (212.10, 83.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694152 reads in 189.302 CPU sec, 6.029 real sec [M::mem_process_seqs] Processed 694354 reads in 128.500 CPU sec, 4.050 real sec [M::process] read 693590 sequences (100000259 bp) in 2.878 CPU sec, 3.018 real sec... sorting block 88, #reads = 1081093 mem = 536878849 peak = 771190784 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284361, 1, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 237, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (243.76, 144.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (293.07, 149.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 218, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (224.54, 96.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693694 sequences (100000093 bp) in 3.194 CPU sec, 3.388 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284202, 3, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 301, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1481) [M::mem_pestat] mean and std.dev: (261.35, 148.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.61, 149.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 215, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 486) [M::mem_pestat] mean and std.dev: (230.08, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693954 reads in 188.707 CPU sec, 6.003 real sec [M::mem_process_seqs] Processed 693590 reads in 125.946 CPU sec, 3.972 real sec [M::process] read 693464 sequences (100000042 bp) in 2.493 CPU sec, 2.582 real sec... sorting block 89, #reads = 1079026 mem = 536877569 peak = 771190784 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284310, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 212, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (185.29, 64.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (292.49, 148.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 261, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (308.90, 205.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693636 sequences (100000065 bp) in 3.176 CPU sec, 3.359 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284278, 3, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 185, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (224.56, 100.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (293.38, 148.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 215, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (224.95, 105.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693694 reads in 190.577 CPU sec, 6.054 real sec sorting block 90, #reads = 1080486 mem = 536878057 peak = 771190784 [M::process] read 693038 sequences (100000010 bp) in 2.191 CPU sec, 2.215 real sec... [M::mem_process_seqs] Processed 693464 reads in 125.773 CPU sec, 3.968 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284078, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 247, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (260.70, 155.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (292.85, 147.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 212, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (247.48, 119.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 91, #reads = 1079363 mem = 536879376 peak = 787070976 [M::process] read 693680 sequences (100000109 bp) in 3.857 CPU sec, 4.111 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284206, 2, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 193, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (259.13, 151.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (294.26, 148.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 288, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (257.59, 132.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693636 reads in 188.692 CPU sec, 6.006 real sec [M::mem_process_seqs] Processed 693038 reads in 124.886 CPU sec, 3.949 real sec [M::process] read 693066 sequences (100000280 bp) in 3.278 CPU sec, 3.480 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284169, 3, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 204, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1347) [M::mem_pestat] mean and std.dev: (343.87, 256.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (293.26, 148.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 283, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 588) [M::mem_pestat] mean and std.dev: (276.53, 71.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 92, #reads = 1079833 mem = 536878279 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284273, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (155.59, 43.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (295.95, 150.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 243, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (260.43, 131.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692546 sequences (100000163 bp) in 3.864 CPU sec, 4.150 real sec... [M::mem_process_seqs] Processed 693680 reads in 191.239 CPU sec, 6.131 real sec [M::mem_process_seqs] Processed 693066 reads in 127.899 CPU sec, 4.056 real sec sorting block 93, #reads = 1079747 mem = 536879823 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283651, 3, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 345, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998) [M::mem_pestat] mean and std.dev: (324.25, 169.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (297.62, 151.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (251.16, 113.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692548 sequences (100000011 bp) in 3.910 CPU sec, 4.259 real sec... [M::mem_process_seqs] Processed 692546 reads in 106.573 CPU sec, 3.528 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284500, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 179, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (177.08, 92.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (299.62, 152.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 235, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (273.82, 139.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 94, #reads = 1079766 mem = 536878519 peak = 787070976 [M::process] read 699548 sequences (100000027 bp) in 3.030 CPU sec, 3.278 real sec... [M::process] read 705260 sequences (100000175 bp) in 2.830 CPU sec, 2.964 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285836, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 362, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322) [M::mem_pestat] mean and std.dev: (333.43, 190.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (277.17, 144.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 202, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (235.30, 91.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699548 reads in 114.944 CPU sec, 3.626 real sec sorting block 95, #reads = 1078911 mem = 536881390 peak = 787070976 [M::process] read 705170 sequences (100000246 bp) in 2.958 CPU sec, 3.109 real sec... [M::mem_process_seqs] Processed 692548 reads in 295.612 CPU sec, 9.495 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 288501, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 268, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (229.54, 104.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 229, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (258.31, 133.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 200, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (240.38, 148.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 703046 sequences (100000233 bp) in 3.681 CPU sec, 3.931 real sec... [M::mem_process_seqs] Processed 705170 reads in 115.181 CPU sec, 3.692 real sec sorting block 96, #reads = 1081217 mem = 536879190 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 288774, 0, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 237, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (233.50, 102.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 228, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (257.17, 133.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 176, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (232.33, 121.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 701646 sequences (100000180 bp) in 2.921 CPU sec, 3.077 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 287992, 3, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 325, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (301.35, 161.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (263.18, 135.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (241.39, 125.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705260 reads in 345.335 CPU sec, 10.972 real sec [M::mem_process_seqs] Processed 703046 reads in 139.590 CPU sec, 4.420 real sec sorting block 97, #reads = 1084430 mem = 536878231 peak = 787070976 [M::process] read 700600 sequences (100000286 bp) in 2.830 CPU sec, 2.979 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 287760, 4, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (242.12, 116.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (268.82, 138.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 257, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (259.46, 127.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 699942 sequences (100000181 bp) in 3.090 CPU sec, 3.294 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 287693, 4, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (248.96, 114.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (272.70, 140.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 246, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (252.33, 91.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701646 reads in 195.332 CPU sec, 6.219 real sec [M::mem_process_seqs] Processed 700600 reads in 129.539 CPU sec, 4.089 real sec sorting block 98, #reads = 1082389 mem = 536877812 peak = 787070976 [M::process] read 699368 sequences (100000219 bp) in 2.601 CPU sec, 2.717 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 286614, 0, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 260, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (282.67, 189.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (274.90, 142.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 264, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (319.92, 189.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 99, #reads = 1082946 mem = 536878899 peak = 787070976 [M::process] read 698580 sequences (100000271 bp) in 3.072 CPU sec, 3.259 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287040, 7, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (210.00, 83.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (277.16, 143.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 188, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (196.27, 89.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699942 reads in 196.389 CPU sec, 6.249 real sec [M::process] read 698590 sequences (100000078 bp) in 2.207 CPU sec, 2.221 real sec... [M::mem_process_seqs] Processed 699368 reads in 129.496 CPU sec, 4.089 real sec sorting block 100, #reads = 1082149 mem = 536877742 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 286155, 3, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 303, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (253.07, 99.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 253, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (280.61, 144.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 207, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (271.65, 165.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 698162 sequences (100000019 bp) in 2.891 CPU sec, 3.101 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 286784, 9, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983) [M::mem_pestat] mean and std.dev: (379.41, 443.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (278.93, 143.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 210, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 453) [M::mem_pestat] mean and std.dev: (240.37, 81.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698580 reads in 190.685 CPU sec, 6.060 real sec [M::process] read 697432 sequences (100000024 bp) in 2.229 CPU sec, 2.239 real sec... [M::mem_process_seqs] Processed 698590 reads in 126.498 CPU sec, 3.997 real sec sorting block 101, #reads = 1082747 mem = 536879567 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286524, 2, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 129, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (147.93, 45.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (281.12, 144.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 190, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (241.12, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697108 sequences (100000176 bp) in 2.925 CPU sec, 3.052 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 286461, 3, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 160, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (236.78, 113.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (284.14, 145.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 262, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (278.50, 128.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696538 sequences (100000136 bp) in 2.304 CPU sec, 2.312 real sec... [M::mem_process_seqs] Processed 698162 reads in 190.087 CPU sec, 6.031 real sec sorting block 102, #reads = 1082870 mem = 536878787 peak = 787070976 [M::mem_process_seqs] Processed 697432 reads in 125.855 CPU sec, 3.980 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 286203, 4, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 239, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (294.71, 207.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (285.40, 146.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 225, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (325.04, 232.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695918 sequences (100000057 bp) in 3.005 CPU sec, 3.188 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285716, 3, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 429, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 717) [M::mem_pestat] mean and std.dev: (330.00, 127.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (288.01, 147.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 262, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (265.45, 97.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695464 sequences (100000123 bp) in 2.010 CPU sec, 2.010 real sec... [M::mem_process_seqs] Processed 697108 reads in 191.002 CPU sec, 6.066 real sec sorting block 103, #reads = 1081647 mem = 536883864 peak = 787070976 [M::mem_process_seqs] Processed 696538 reads in 127.396 CPU sec, 4.023 real sec sorting block 104, #reads = 1080534 mem = 536882606 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 286066, 3, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 187, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (216.42, 103.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (289.27, 148.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 306, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (245.25, 77.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695108 sequences (100000070 bp) in 3.620 CPU sec, 3.825 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 285322, 5, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 210, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (212.10, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (290.10, 148.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (245.94, 109.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695918 reads in 192.490 CPU sec, 6.099 real sec [M::process] read 694798 sequences (100000185 bp) in 2.181 CPU sec, 2.202 real sec... [M::mem_process_seqs] Processed 695464 reads in 127.100 CPU sec, 4.009 real sec sorting block 105, #reads = 1080436 mem = 536876672 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284923, 1, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 200, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (232.63, 107.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 263, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (290.01, 147.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 190, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (232.67, 156.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694566 sequences (100000087 bp) in 3.033 CPU sec, 3.257 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284508, 5, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 179, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007) [M::mem_pestat] mean and std.dev: (255.52, 181.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (291.18, 148.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 299, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (269.30, 108.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694376 sequences (100000173 bp) in 1.892 CPU sec, 1.901 real sec... [M::mem_process_seqs] Processed 695108 reads in 190.559 CPU sec, 6.063 real sec [M::mem_process_seqs] Processed 694798 reads in 126.615 CPU sec, 4.004 real sec sorting block 106, #reads = 1080233 mem = 536878982 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284478, 4, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 213, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (251.96, 173.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (291.88, 149.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 252, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1973) [M::mem_pestat] mean and std.dev: (421.20, 365.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694390 sequences (100000165 bp) in 3.437 CPU sec, 3.673 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285065, 2, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 238, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (210.35, 100.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (291.77, 149.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 194, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (248.67, 167.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694566 reads in 188.976 CPU sec, 6.014 real sec sorting block 107, #reads = 1079784 mem = 536879170 peak = 787070976 [M::mem_process_seqs] Processed 694376 reads in 125.647 CPU sec, 3.961 real sec [M::process] read 694508 sequences (100000252 bp) in 2.530 CPU sec, 2.613 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284743, 1, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 275, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (282.06, 156.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (292.96, 149.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 207, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (255.18, 129.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 108, #reads = 1079949 mem = 536880741 peak = 787070976 [M::process] read 693392 sequences (100000077 bp) in 2.891 CPU sec, 3.105 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285226, 3, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 234, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (241.75, 86.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (291.17, 148.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 242, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (251.18, 113.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694390 reads in 188.421 CPU sec, 5.999 real sec [M::mem_process_seqs] Processed 694508 reads in 126.070 CPU sec, 3.968 real sec [M::process] read 693068 sequences (100000202 bp) in 2.981 CPU sec, 3.098 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284452, 4, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 144, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (238.39, 196.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 109, #reads = 1081011 mem = 536876665 peak = 787070976 [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (295.36, 150.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 271, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (264.28, 89.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692788 sequences (100000044 bp) in 3.131 CPU sec, 3.371 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284498, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 190, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (224.87, 144.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (295.12, 149.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (242.73, 95.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693392 reads in 185.879 CPU sec, 5.934 real sec [M::mem_process_seqs] Processed 693068 reads in 123.629 CPU sec, 3.905 real sec [M::process] read 693246 sequences (100000177 bp) in 2.518 CPU sec, 2.644 real sec... sorting block 110, #reads = 1080095 mem = 536879489 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284214, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 246, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (226.25, 124.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (295.88, 150.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 319, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (279.85, 138.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693948 sequences (100000079 bp) in 3.013 CPU sec, 3.207 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284184, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 173, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (166.96, 54.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (296.13, 150.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 297, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (280.45, 126.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692788 reads in 187.537 CPU sec, 6.037 real sec [M::mem_process_seqs] Processed 693246 reads in 126.787 CPU sec, 4.011 real sec sorting block 111, #reads = 1081071 mem = 536879126 peak = 787070976 [M::process] read 698594 sequences (100000058 bp) in 3.207 CPU sec, 3.425 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284010, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (186.40, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (292.90, 150.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 201, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (220.77, 74.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 112, #reads = 1079552 mem = 536882379 peak = 787070976 [M::process] read 702822 sequences (100000065 bp) in 2.902 CPU sec, 3.078 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 286069, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 295, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (275.86, 103.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (277.46, 143.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 233, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (252.27, 102.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693948 reads in 200.291 CPU sec, 6.391 real sec [M::mem_process_seqs] Processed 698594 reads in 136.176 CPU sec, 4.297 real sec [M::process] read 701550 sequences (100000215 bp) in 2.108 CPU sec, 2.193 real sec... sorting block 113, #reads = 1074109 mem = 536880958 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 287418, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 217, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (187.93, 66.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (265.26, 137.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 187, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (212.14, 65.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 700494 sequences (100000078 bp) in 2.782 CPU sec, 3.001 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286925, 3, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 359, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3827) [M::mem_pestat] mean and std.dev: (757.73, 665.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (269.36, 139.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 187, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (219.88, 101.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702822 reads in 177.460 CPU sec, 5.651 real sec [M::mem_process_seqs] Processed 701550 reads in 117.043 CPU sec, 3.682 real sec [M::process] read 699796 sequences (100000038 bp) in 2.331 CPU sec, 2.383 real sec... sorting block 114, #reads = 1087588 mem = 536880166 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 287059, 3, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 369, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (378.07, 318.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (273.36, 141.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 214, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (287.48, 152.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 699008 sequences (100000159 bp) in 3.285 CPU sec, 3.463 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 286341, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (187.32, 85.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (274.45, 141.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 279, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2083) [M::mem_pestat] mean and std.dev: (495.55, 489.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2719) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700494 reads in 177.748 CPU sec, 5.658 real sec [M::mem_process_seqs] Processed 699796 reads in 117.233 CPU sec, 3.698 real sec [M::process] read 698144 sequences (100000092 bp) in 2.554 CPU sec, 2.627 real sec... sorting block 115, #reads = 1086773 mem = 536884575 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 286478, 5, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 166, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416) [M::mem_pestat] mean and std.dev: (341.56, 338.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (276.97, 142.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 281, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (279.45, 108.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697550 sequences (100000230 bp) in 2.849 CPU sec, 3.028 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286214, 6, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 193, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 301) [M::mem_pestat] mean and std.dev: (181.56, 30.95) [M::mem_pestat] low and high boundaries for proper pairs: (58, 337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (279.73, 143.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 206, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (221.15, 83.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699008 reads in 180.149 CPU sec, 5.750 real sec sorting block 116, #reads = 1086681 mem = 536882788 peak = 787070976 [M::mem_process_seqs] Processed 698144 reads in 118.592 CPU sec, 3.744 real sec [M::process] read 697130 sequences (100000179 bp) in 2.440 CPU sec, 2.516 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 286307, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 203, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (199.21, 81.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 255, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (281.11, 142.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 250, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (232.10, 70.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 117, #reads = 1087141 mem = 536881189 peak = 787070976 [M::process] read 696184 sequences (100000091 bp) in 3.262 CPU sec, 3.457 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285431, 9, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 221, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (302.83, 154.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (283.20, 143.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (247.53, 126.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697550 reads in 183.457 CPU sec, 5.841 real sec [M::mem_process_seqs] Processed 697130 reads in 122.976 CPU sec, 3.882 real sec [M::process] read 697256 sequences (100000012 bp) in 2.523 CPU sec, 2.604 real sec... sorting block 118, #reads = 1086087 mem = 536879949 peak = 787070976 [M::process] read 697004 sequences (100000094 bp) in 3.159 CPU sec, 3.347 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286183, 3, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 257, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 437) [M::mem_pestat] mean and std.dev: (221.36, 56.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (283.56, 144.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 275, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (316.52, 170.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697256 reads in 99.207 CPU sec, 3.120 real sec [M::process] read 696586 sequences (100000130 bp) in 2.521 CPU sec, 2.626 real sec... sorting block 119, #reads = 1084112 mem = 536884566 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 285831, 7, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 215, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (187.24, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (285.36, 145.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 247, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (309.81, 173.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285770, 2, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 222, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (221.11, 65.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (283.78, 144.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 242, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 497) [M::mem_pestat] mean and std.dev: (239.67, 46.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696184 reads in 284.019 CPU sec, 9.016 real sec [M::mem_process_seqs] Processed 697004 reads in 140.406 CPU sec, 4.430 real sec [M::process] read 696090 sequences (100000119 bp) in 2.475 CPU sec, 2.551 real sec... sorting block 120, #reads = 1082505 mem = 536878480 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 285932, 1, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 195, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (209.88, 64.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (284.27, 144.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 1671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4673) [M::mem_pestat] mean and std.dev: (626.41, 735.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696150 sequences (100000029 bp) in 3.106 CPU sec, 3.307 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 285315, 3, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 224, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (261.96, 116.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (286.31, 144.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 258, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (298.41, 173.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696586 reads in 180.903 CPU sec, 5.765 real sec [M::mem_process_seqs] Processed 696090 reads in 120.106 CPU sec, 3.784 real sec [M::process] read 697166 sequences (100000269 bp) in 2.585 CPU sec, 2.686 real sec... sorting block 121, #reads = 1084823 mem = 536878676 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285066, 5, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 177, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (181.57, 88.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (285.30, 143.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (219.20, 93.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696268 sequences (100000051 bp) in 3.075 CPU sec, 3.243 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285962, 7, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 201, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (214.83, 91.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 255, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (280.64, 142.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 210, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.92, 103.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696150 reads in 187.955 CPU sec, 5.973 real sec [M::process] read 694926 sequences (100000088 bp) in 2.230 CPU sec, 2.259 real sec... [M::mem_process_seqs] Processed 697166 reads in 124.577 CPU sec, 3.924 real sec sorting block 122, #reads = 1084250 mem = 536878474 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 285117, 2, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 233, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (239.10, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (286.67, 145.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 253, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (237.47, 115.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 123, #reads = 1083032 mem = 536878885 peak = 787070976 [M::process] read 695370 sequences (100000258 bp) in 3.034 CPU sec, 3.233 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285063, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 233, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (257.17, 140.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (290.08, 146.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 240, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (256.33, 100.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696268 reads in 196.234 CPU sec, 6.259 real sec [M::process] read 694604 sequences (100000293 bp) in 2.320 CPU sec, 2.351 real sec... [M::mem_process_seqs] Processed 694926 reads in 131.008 CPU sec, 4.134 real sec sorting block 124, #reads = 1082447 mem = 536877288 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284822, 4, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 303, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (326.41, 149.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (289.03, 146.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 235, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1323) [M::mem_pestat] mean and std.dev: (337.40, 250.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694850 sequences (100000046 bp) in 3.346 CPU sec, 3.498 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284721, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 172, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (239.00, 167.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (291.77, 148.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 290, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (312.28, 143.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695370 reads in 190.639 CPU sec, 6.047 real sec [M::process] read 695380 sequences (100000116 bp) in 2.572 CPU sec, 2.603 real sec... [M::mem_process_seqs] Processed 694604 reads in 125.687 CPU sec, 3.947 real sec sorting block 125, #reads = 1079847 mem = 536877759 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284304, 7, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 271, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (235.74, 102.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (290.95, 148.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 354, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (305.56, 94.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694434 sequences (100000066 bp) in 3.572 CPU sec, 3.828 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284601, 5, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 238, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (230.77, 95.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (286.85, 145.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 258, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (257.86, 104.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694850 reads in 191.639 CPU sec, 6.114 real sec [M::mem_process_seqs] Processed 695380 reads in 127.748 CPU sec, 4.031 real sec [M::process] read 694414 sequences (100000002 bp) in 2.548 CPU sec, 2.644 real sec... sorting block 126, #reads = 1079996 mem = 536879157 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284733, 8, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 167, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1647) [M::mem_pestat] mean and std.dev: (357.20, 302.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (289.37, 145.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 264, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (276.20, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694348 sequences (100000175 bp) in 2.877 CPU sec, 3.021 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284400, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 231, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2922) [M::mem_pestat] mean and std.dev: (685.35, 803.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (292.18, 148.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 252, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (261.03, 152.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694434 reads in 188.570 CPU sec, 5.983 real sec [M::process] read 694230 sequences (100000170 bp) in 2.146 CPU sec, 2.150 real sec... sorting block 127, #reads = 1079303 mem = 536879364 peak = 787070976 [M::mem_process_seqs] Processed 694414 reads in 125.541 CPU sec, 3.955 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284456, 3, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 253, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (257.56, 113.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 128, #reads = 1079636 mem = 536878791 peak = 787070976 [M::mem_pestat] (25, 50, 75) percentile: (172, 263, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (290.66, 148.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 207, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 369) [M::mem_pestat] mean and std.dev: (191.55, 44.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693918 sequences (100000089 bp) in 3.389 CPU sec, 3.551 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284516, 0, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 183, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (226.19, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (291.63, 148.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 249, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (279.95, 156.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693912 sequences (100000112 bp) in 2.190 CPU sec, 2.190 real sec... [M::mem_process_seqs] Processed 694348 reads in 191.786 CPU sec, 6.071 real sec [M::mem_process_seqs] Processed 694230 reads in 129.175 CPU sec, 4.079 real sec sorting block 129, #reads = 1078354 mem = 536878614 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284398, 1, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 125, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (146.75, 44.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (292.12, 148.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 217, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (204.00, 70.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696516 sequences (100000167 bp) in 2.753 CPU sec, 2.928 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284159, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 245, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (225.04, 90.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (292.45, 148.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 386, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (376.63, 202.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 708048 sequences (100000129 bp) in 2.370 CPU sec, 2.370 real sec... [M::mem_process_seqs] Processed 693918 reads in 201.198 CPU sec, 6.386 real sec [M::mem_process_seqs] Processed 693912 reads in 135.838 CPU sec, 4.299 real sec sorting block 130, #reads = 1077041 mem = 536879162 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284638, 7, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 238, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (216.69, 88.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (283.12, 145.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 225, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (245.18, 109.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 705676 sequences (100000267 bp) in 3.553 CPU sec, 3.733 real sec... [M::process] read 703578 sequences (100000282 bp) in 2.040 CPU sec, 2.045 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 289408, 2, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 210, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (203.61, 85.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 222, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (249.93, 128.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 254, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (251.77, 147.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696516 reads in 223.648 CPU sec, 7.099 real sec sorting block 131, #reads = 1072773 mem = 536879430 peak = 787070976 [M::mem_process_seqs] Processed 708048 reads in 140.247 CPU sec, 4.453 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 289003, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (234.60, 135.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 229, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (258.00, 133.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 300, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (278.66, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 132, #reads = 1076880 mem = 536879727 peak = 787070976 [M::process] read 702646 sequences (100000009 bp) in 3.094 CPU sec, 3.261 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 288185, 6, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 189, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (189.38, 72.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (263.21, 134.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 268, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (278.39, 103.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 701594 sequences (100000180 bp) in 2.339 CPU sec, 2.348 real sec... [M::mem_process_seqs] Processed 705676 reads in 205.562 CPU sec, 6.522 real sec [M::mem_process_seqs] Processed 703578 reads in 135.296 CPU sec, 4.274 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 287800, 5, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 257, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (234.76, 92.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (265.57, 135.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 360, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (331.87, 114.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 133, #reads = 1082942 mem = 536886070 peak = 787070976 [M::process] read 700120 sequences (100000106 bp) in 3.825 CPU sec, 4.070 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286942, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 206, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653) [M::mem_pestat] mean and std.dev: (265.40, 195.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (268.85, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 282, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (303.36, 133.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702646 reads in 206.785 CPU sec, 6.584 real sec [M::mem_process_seqs] Processed 701594 reads in 139.756 CPU sec, 4.415 real sec [M::process] read 699268 sequences (100000121 bp) in 2.477 CPU sec, 2.555 real sec... sorting block 134, #reads = 1081166 mem = 536879278 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 287106, 4, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 224, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (330.42, 223.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (274.37, 139.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 226, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (239.60, 92.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 698672 sequences (100000017 bp) in 2.948 CPU sec, 3.112 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 286244, 7, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 266, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 519) [M::mem_pestat] mean and std.dev: (275.45, 104.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 250, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (276.07, 140.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 261, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (247.45, 88.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700120 reads in 200.481 CPU sec, 6.377 real sec [M::process] read 698268 sequences (100000032 bp) in 2.260 CPU sec, 2.267 real sec... sorting block 135, #reads = 1080778 mem = 536880573 peak = 787070976 [M::mem_process_seqs] Processed 699268 reads in 134.160 CPU sec, 4.235 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 286008, 5, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 366, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (327.93, 133.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 252, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (278.76, 141.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 212, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (30, 395) [M::mem_pestat] mean and std.dev: (212.68, 66.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 136, #reads = 1081214 mem = 536880632 peak = 787070976 [M::process] read 699270 sequences (100000267 bp) in 3.474 CPU sec, 3.646 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 285940, 2, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 269, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (265.04, 107.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (280.25, 142.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 207, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (283.44, 165.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 698090 sequences (100000031 bp) in 2.298 CPU sec, 2.305 real sec... [M::mem_process_seqs] Processed 698672 reads in 199.552 CPU sec, 6.317 real sec [M::mem_process_seqs] Processed 698268 reads in 130.170 CPU sec, 4.109 real sec sorting block 137, #reads = 1079654 mem = 536881388 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 286627, 9, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 202, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (206.81, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 251, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (278.30, 142.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 237, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668) [M::mem_pestat] mean and std.dev: (408.19, 326.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697422 sequences (100000283 bp) in 2.985 CPU sec, 3.148 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 286033, 2, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 196, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 388) [M::mem_pestat] mean and std.dev: (187.59, 44.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (282.12, 144.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 256, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (248.59, 129.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 699786 sequences (100000121 bp) in 2.199 CPU sec, 2.207 real sec... [M::mem_process_seqs] Processed 699270 reads in 202.056 CPU sec, 6.413 real sec [M::mem_process_seqs] Processed 698090 reads in 130.715 CPU sec, 4.137 real sec sorting block 138, #reads = 1079585 mem = 536879368 peak = 787070976 [M::process] read 696378 sequences (100000068 bp) in 2.844 CPU sec, 2.992 real sec... [M::process] read 695490 sequences (100000017 bp) in 2.075 CPU sec, 2.085 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 286124, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 176, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (178.16, 55.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.84, 140.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 241, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (277.32, 170.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699786 reads in 114.728 CPU sec, 3.638 real sec sorting block 139, #reads = 1079591 mem = 536878631 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 286250, 12, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 224, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (252.78, 122.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (283.84, 144.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (154, 281, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (208.82, 86.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 307, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1790) [M::mem_pestat] mean and std.dev: (384.85, 312.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285286, 6, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217) [M::mem_pestat] mean and std.dev: (244.33, 146.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (283.47, 142.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 224, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (242.96, 110.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1420) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697422 reads in 307.371 CPU sec, 10.037 real sec [M::process] read 694752 sequences (100000192 bp) in 2.563 CPU sec, 2.572 real sec... [M::mem_process_seqs] Processed 696378 reads in 156.064 CPU sec, 4.968 real sec sorting block 140, #reads = 1079765 mem = 536878201 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 285430, 4, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 257, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (276.48, 154.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (286.18, 143.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 272, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209) [M::mem_pestat] mean and std.dev: (328.74, 195.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1548) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694638 sequences (100000099 bp) in 3.167 CPU sec, 3.405 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284884, 2, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 180, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (188.58, 69.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 263, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (288.00, 144.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 267, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (230.29, 82.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694336 sequences (100000142 bp) in 2.059 CPU sec, 2.058 real sec... [M::mem_process_seqs] Processed 695490 reads in 196.097 CPU sec, 6.226 real sec sorting block 141, #reads = 1078609 mem = 536884996 peak = 787070976 [M::mem_process_seqs] Processed 694752 reads in 130.574 CPU sec, 4.129 real sec [M::process] read 694798 sequences (100000143 bp) in 3.769 CPU sec, 3.987 real sec... [M::process] read 693762 sequences (100000100 bp) in 2.170 CPU sec, 2.164 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284407, 2, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (240.85, 102.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 261, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (284.81, 141.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 219, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (217.30, 71.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694798 reads in 99.815 CPU sec, 3.368 real sec sorting block 142, #reads = 1079611 mem = 536880792 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283831, 6, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (263.17, 166.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (287.75, 143.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 236, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1384) [M::mem_pestat] mean and std.dev: (431.92, 388.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1985) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694336 reads in 207.755 CPU sec, 7.554 real sec sorting block 143, #reads = 1078633 mem = 536878901 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284646, 8, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 172, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (160.79, 46.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (287.44, 143.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::process] read 693690 sequences (100000197 bp) in 2.740 CPU sec, 2.943 real sec... [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 208, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (224.68, 94.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283994, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 184, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (237.49, 114.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 263, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (286.07, 141.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 197, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (245.59, 136.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694638 reads in 398.914 CPU sec, 13.630 real sec [M::mem_process_seqs] Processed 693762 reads in 147.116 CPU sec, 4.839 real sec [M::process] read 693424 sequences (100000006 bp) in 2.051 CPU sec, 2.159 real sec... sorting block 144, #reads = 1079978 mem = 536878576 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283790, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 231, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (264.44, 122.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 265, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (288.68, 143.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 265, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (226.28, 100.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693134 sequences (100000174 bp) in 2.760 CPU sec, 2.950 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284171, 2, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 249, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (277.68, 163.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 265, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (288.38, 143.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 227, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (249.33, 80.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693690 reads in 182.756 CPU sec, 5.821 real sec [M::process] read 692864 sequences (100000164 bp) in 2.245 CPU sec, 2.276 real sec... [M::mem_process_seqs] Processed 693424 reads in 123.351 CPU sec, 3.895 real sec sorting block 145, #reads = 1078796 mem = 536878711 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284076, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (173.63, 67.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (293.29, 147.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 254, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (262.82, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692366 sequences (100000069 bp) in 2.704 CPU sec, 2.904 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284254, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 228, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (210.74, 65.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (294.97, 148.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 219, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (223.62, 78.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692334 sequences (100000017 bp) in 1.660 CPU sec, 1.654 real sec... [M::mem_process_seqs] Processed 693134 reads in 177.193 CPU sec, 5.645 real sec sorting block 146, #reads = 1079232 mem = 536879277 peak = 787070976 [M::mem_process_seqs] Processed 692864 reads in 118.172 CPU sec, 4.000 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284521, 5, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 173, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133) [M::mem_pestat] mean and std.dev: (282.83, 214.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (295.45, 148.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 208, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2088) [M::mem_pestat] mean and std.dev: (444.94, 463.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 147, #reads = 1080146 mem = 536877401 peak = 787070976 [M::process] read 517598 sequences (74776391 bp) in 2.448 CPU sec, 2.611 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283570, 5, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 327, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (332.81, 192.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (299.25, 151.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 276, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (352.56, 235.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692366 reads in 177.084 CPU sec, 5.941 real sec [M::mem_process_seqs] Processed 692334 reads in 113.403 CPU sec, 3.656 real sec sorting block 148, #reads = 1080547 mem = 536882259 peak = 787070976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 212298, 3, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 299, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (285.61, 152.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (299.07, 151.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 188, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (202.10, 69.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 517598 reads in 76.728 CPU sec, 2.541 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 161163480 overall: 69724958720 mem 25244.792 user 1064.329 sys 940.210 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_87O-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1136_87O-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_87O-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_87O-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_87O-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 940.242 sec; CPU: 26309.126 sec sorting block 149, #reads = 883720 mem = 440016035 peak = 787070976 uniq header count 1 merging 150 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 8765655312 output reads: 162158562 bam_mem_sort: 150 calls 106.203 user 4.442 sys 111.266 real bam_write: 150 calls 58.222 user 5.296 sys 73.322 real execute: 1 calls 50.989 user 2.595 sys 1002.632 real merge_files: 1 calls 50.955 user 2.568 sys 61.806 real parse_chunk: 2398 calls 110.685 user 30.749 sys 146.587 real read_chunk: 6477 calls 77.295 user 56.409 sys 850.694 real sort_block: 1 calls 56.281 user 55.381 sys 820.782 real write_chunk: 4077 calls 50.563 user 2.119 sys 60.620 real overall: 3001397248 mem 2468.947 user 124.921 sys 1002.654 real ls: cannot access '*.cram': No such file or directory