cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1662.765 CPU sec, 59.074 real sec
[M::process] read 702868 sequences (100000055 bp) in 0.982 CPU sec, 0.988 real sec...
[M::process] read 702112 sequences (100000018 bp) in 1.271 CPU sec, 1.302 real sec...
[M::process] read 701720 sequences (100000078 bp) in 1.354 CPU sec, 1.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292149, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.28, 114.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 236, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (97, 412)
[M::mem_pestat] mean and std.dev: (239.44, 33.45)
[M::mem_pestat] low and high boundaries for proper pairs: (34, 475)
[M::mem_pestat] skip orientation RR
[M::process] read 700994 sequences (100000276 bp) in 1.254 CPU sec, 1.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291420, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.87, 116.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292025, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (241.92, 115.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702868 reads in 225.968 CPU sec, 7.242 real sec
[M::mem_process_seqs] Processed 701720 reads in 143.445 CPU sec, 4.644 real sec
[M::mem_process_seqs] Processed 702112 reads in 155.067 CPU sec, 5.037 real sec
sorting block 0, #reads = 1096290 mem = 536886182 peak = 600539136
[M::process] read 700034 sequences (100000151 bp) in 1.817 CPU sec, 1.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291820, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 204, 1035)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2791)
[M::mem_pestat] mean and std.dev: (645.60, 761.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3692)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.36, 117.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699470 sequences (100000062 bp) in 1.303 CPU sec, 1.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291309, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.64, 119.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700994 reads in 131.848 CPU sec, 4.195 real sec
sorting block 1, #reads = 1095305 mem = 536876434 peak = 910770176
[M::process] read 698978 sequences (100000301 bp) in 1.247 CPU sec, 1.257 real sec...
[M::mem_process_seqs] Processed 700034 reads in 82.785 CPU sec, 2.614 real sec
sorting block 2, #reads = 1094264 mem = 536879440 peak = 973914112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291695, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.69, 120.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698484 sequences (100000016 bp) in 1.592 CPU sec, 1.841 real sec...
[M::process] read 698716 sequences (100000111 bp) in 1.261 CPU sec, 1.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290876, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.69, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699470 reads in 130.793 CPU sec, 4.171 real sec
[M::mem_process_seqs] Processed 698978 reads in 81.818 CPU sec, 2.608 real sec
sorting block 3, #reads = 1094697 mem = 536877154 peak = 973914112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290754, 10, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.10, 119.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 264, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (325.00, 185.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 455, 997)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2575)
[M::mem_pestat] mean and std.dev: (476.27, 335.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3364)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698160 sequences (100000073 bp) in 1.810 CPU sec, 1.890 real sec...
[M::process] read 698514 sequences (100000232 bp) in 1.230 CPU sec, 1.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291020, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.70, 120.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698484 reads in 131.857 CPU sec, 4.202 real sec
[M::mem_process_seqs] Processed 698716 reads in 82.540 CPU sec, 2.630 real sec
sorting block 4, #reads = 1093734 mem = 536878549 peak = 973914112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290760, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (249.04, 118.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 268, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (247.50, 84.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RR
[M::process] read 698252 sequences (100000093 bp) in 1.779 CPU sec, 1.861 real sec...
[M::process] read 697610 sequences (100000177 bp) in 1.328 CPU sec, 1.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290990, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (248.39, 118.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698160 reads in 132.795 CPU sec, 4.235 real sec
sorting block 5, #reads = 1093695 mem = 536879444 peak = 973914112
[M::mem_process_seqs] Processed 698514 reads in 83.645 CPU sec, 2.661 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290575, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.45, 119.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 6, #reads = 1093439 mem = 536878628 peak = 973914112
[M::process] read 697130 sequences (100000123 bp) in 2.060 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290994, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.30, 120.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696848 sequences (100000242 bp) in 1.393 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 698252 reads in 134.350 CPU sec, 4.282 real sec
[M::mem_process_seqs] Processed 697610 reads in 83.749 CPU sec, 2.662 real sec
sorting block 7, #reads = 1094007 mem = 536879845 peak = 973914112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290809, 8, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 420, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (364.23, 189.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.01, 119.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696218 sequences (100000272 bp) in 1.916 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290165, 4, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.58, 120.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 643, 885)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2135)
[M::mem_pestat] mean and std.dev: (618.36, 473.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2760)
[M::mem_pestat] skip orientation RR
[M::process] read 696124 sequences (100000036 bp) in 1.359 CPU sec, 1.361 real sec...
[M::mem_process_seqs] Processed 697130 reads in 133.696 CPU sec, 4.271 real sec
[M::mem_process_seqs] Processed 696848 reads in 83.810 CPU sec, 2.667 real sec
sorting block 8, #reads = 1093017 mem = 536881880 peak = 973914112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290596, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 378, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (344.45, 181.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.22, 121.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 225, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (212.09, 80.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 695926 sequences (100000204 bp) in 2.171 CPU sec, 2.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290111, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 239, 1413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3859)
[M::mem_pestat] mean and std.dev: (555.08, 570.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5082)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.38, 122.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 401, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364)
[M::mem_pestat] mean and std.dev: (334.50, 167.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1753)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 703564 sequences (100000119 bp) in 1.380 CPU sec, 1.378 real sec...
[M::mem_process_seqs] Processed 696218 reads in 133.197 CPU sec, 4.242 real sec
[M::mem_process_seqs] Processed 696124 reads in 83.167 CPU sec, 2.642 real sec
sorting block 9, #reads = 1091270 mem = 536888657 peak = 973914112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 289718, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 184, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (260.89, 177.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (257.37, 123.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702546 sequences (100000143 bp) in 1.899 CPU sec, 1.995 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292890, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (240.43, 116.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 248, 757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1989)
[M::mem_pestat] mean and std.dev: (458.70, 382.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2605)
[M::mem_pestat] skip orientation RR
[M::process] read 702218 sequences (100000236 bp) in 1.380 CPU sec, 1.388 real sec...
[M::mem_process_seqs] Processed 695926 reads in 134.137 CPU sec, 4.282 real sec
sorting block 10, #reads = 1093114 mem = 536879537 peak = 973914112
[M::mem_process_seqs] Processed 703564 reads in 84.024 CPU sec, 2.669 real sec
sorting block 11, #reads = 1094400 mem = 536878716 peak = 999215104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 292072, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 182, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (189.38, 69.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 684)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.69, 117.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 182, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (206.50, 119.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 701274 sequences (100000009 bp) in 2.277 CPU sec, 2.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291895, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.65, 117.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700134 sequences (100000066 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 702546 reads in 134.021 CPU sec, 4.268 real sec
[M::mem_process_seqs] Processed 702218 reads in 83.885 CPU sec, 2.660 real sec
sorting block 12, #reads = 1097663 mem = 536881552 peak = 999215104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291748, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.65, 118.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700046 sequences (100000128 bp) in 1.969 CPU sec, 2.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291951, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.20, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699610 sequences (100000283 bp) in 1.420 CPU sec, 1.415 real sec...
[M::mem_process_seqs] Processed 701274 reads in 132.808 CPU sec, 4.239 real sec
sorting block 13, #reads = 1096433 mem = 536879255 peak = 999215104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291547, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (250.14, 122.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699364 sequences (100000147 bp) in 1.621 CPU sec, 1.631 real sec...
[M::mem_process_seqs] Processed 700046 reads in 79.024 CPU sec, 2.770 real sec
[M::mem_process_seqs] Processed 700134 reads in 190.516 CPU sec, 6.327 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291042, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.00, 122.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1096158 mem = 536878335 peak = 999215104
[M::process] read 698946 sequences (100000122 bp) in 2.068 CPU sec, 2.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291579, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.91, 122.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699610 reads in 145.134 CPU sec, 4.632 real sec
[M::mem_process_seqs] Processed 699364 reads in 84.874 CPU sec, 2.694 real sec
[M::process] read 698754 sequences (100000154 bp) in 1.774 CPU sec, 1.872 real sec...
sorting block 15, #reads = 1094701 mem = 536886802 peak = 999215104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291121, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.53, 121.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698986 sequences (100000251 bp) in 2.076 CPU sec, 2.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291235, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.33, 120.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698946 reads in 134.760 CPU sec, 4.294 real sec
[M::process] read 698704 sequences (100000026 bp) in 1.388 CPU sec, 1.394 real sec...
[M::mem_process_seqs] Processed 698754 reads in 84.577 CPU sec, 2.677 real sec
sorting block 16, #reads = 1094837 mem = 536879215 peak = 999215104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290857, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.24, 118.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697954 sequences (100000171 bp) in 2.122 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291434, 3, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 252, 708)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1830)
[M::mem_pestat] mean and std.dev: (441.00, 413.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2391)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (248.23, 118.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698986 reads in 133.252 CPU sec, 4.254 real sec
sorting block 17, #reads = 1093343 mem = 536882567 peak = 999215104
[M::process] read 697918 sequences (100000270 bp) in 1.479 CPU sec, 1.493 real sec...
[M::mem_process_seqs] Processed 698704 reads in 83.858 CPU sec, 2.655 real sec
sorting block 18, #reads = 1093545 mem = 536877006 peak = 1078120448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290393, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 163, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (184.50, 79.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.28, 119.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697192 sequences (100000089 bp) in 2.291 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290373, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.00, 119.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 248, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (295.30, 135.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RR
[M::process] read 697044 sequences (100000244 bp) in 1.420 CPU sec, 1.415 real sec...
[M::mem_process_seqs] Processed 697954 reads in 135.082 CPU sec, 4.303 real sec
[M::mem_process_seqs] Processed 697918 reads in 84.331 CPU sec, 2.674 real sec
sorting block 19, #reads = 1093174 mem = 536889679 peak = 1078120448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290459, 8, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.72, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 254, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104)
[M::mem_pestat] mean and std.dev: (292.77, 176.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1416)
[M::mem_pestat] skip orientation RR
[M::process] read 697160 sequences (100000008 bp) in 2.083 CPU sec, 2.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290519, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.49, 121.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697192 reads in 133.589 CPU sec, 4.259 real sec
[M::process] read 698878 sequences (100000068 bp) in 1.469 CPU sec, 1.476 real sec...
[M::mem_process_seqs] Processed 697044 reads in 83.992 CPU sec, 2.657 real sec
sorting block 20, #reads = 1093424 mem = 536878207 peak = 1078120448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290406, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 341, 1113)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3029)
[M::mem_pestat] mean and std.dev: (498.92, 489.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3987)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.83, 122.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 704368 sequences (100000244 bp) in 2.258 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291091, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.17, 121.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 268, 779)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1925)
[M::mem_pestat] mean and std.dev: (491.77, 462.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2498)
[M::mem_pestat] skip orientation RR
[M::process] read 703984 sequences (100000245 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_process_seqs] Processed 697160 reads in 135.101 CPU sec, 4.298 real sec
sorting block 21, #reads = 1092287 mem = 536879338 peak = 1078120448
[M::mem_process_seqs] Processed 698878 reads in 85.386 CPU sec, 2.706 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 292643, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 335, 1373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3635)
[M::mem_pestat] mean and std.dev: (613.14, 505.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4766)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (239.47, 116.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 22, #reads = 1091573 mem = 536877040 peak = 1078120448
[M::process] read 702780 sequences (100000090 bp) in 2.036 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 293050, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (240.14, 116.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704368 reads in 130.758 CPU sec, 4.172 real sec
[M::process] read 701450 sequences (100000221 bp) in 1.531 CPU sec, 1.547 real sec...
[M::mem_process_seqs] Processed 703984 reads in 83.411 CPU sec, 2.632 real sec
sorting block 23, #reads = 1097926 mem = 536878335 peak = 1078120448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291907, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (242.98, 117.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700630 sequences (100000168 bp) in 2.140 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291880, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.70, 118.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702780 reads in 130.783 CPU sec, 4.171 real sec
[M::mem_process_seqs] Processed 701450 reads in 83.704 CPU sec, 2.638 real sec
[M::process] read 699992 sequences (100000263 bp) in 1.570 CPU sec, 1.606 real sec...
sorting block 24, #reads = 1096506 mem = 536880039 peak = 1078120448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291801, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.24, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699212 sequences (100000120 bp) in 2.114 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291704, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.23, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700630 reads in 129.959 CPU sec, 4.150 real sec
[M::mem_process_seqs] Processed 699992 reads in 82.620 CPU sec, 2.611 real sec
sorting block 25, #reads = 1096323 mem = 536885569 peak = 1078120448
[M::process] read 699426 sequences (100000062 bp) in 1.733 CPU sec, 1.787 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290755, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (330, 330, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 645)
[M::mem_pestat] mean and std.dev: (320.62, 97.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.28, 120.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699130 sequences (100000108 bp) in 2.184 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291584, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.60, 121.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (250.00, 146.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699212 reads in 130.404 CPU sec, 4.150 real sec
sorting block 26, #reads = 1095346 mem = 536882710 peak = 1078120448
[M::mem_process_seqs] Processed 699426 reads in 83.513 CPU sec, 2.632 real sec
[M::process] read 698674 sequences (100000208 bp) in 1.565 CPU sec, 1.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291316, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.22, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1093696 mem = 536878511 peak = 1078120448
[M::process] read 698408 sequences (100000066 bp) in 2.140 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290835, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.74, 118.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (309, 347, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (233, 423)
[M::mem_pestat] mean and std.dev: (329.57, 30.17)
[M::mem_pestat] low and high boundaries for proper pairs: (195, 461)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699130 reads in 131.161 CPU sec, 4.183 real sec
[M::mem_process_seqs] Processed 698674 reads in 83.926 CPU sec, 2.648 real sec
[M::process] read 698498 sequences (100000150 bp) in 1.626 CPU sec, 1.651 real sec...
sorting block 28, #reads = 1094501 mem = 536880165 peak = 1078120448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291015, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 572, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284)
[M::mem_pestat] mean and std.dev: (396.11, 193.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1632)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.58, 118.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698066 sequences (100000149 bp) in 2.216 CPU sec, 2.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290982, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.09, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698408 reads in 131.720 CPU sec, 4.188 real sec
[M::process] read 697494 sequences (100000147 bp) in 1.425 CPU sec, 1.435 real sec...
[M::mem_process_seqs] Processed 698498 reads in 83.985 CPU sec, 2.655 real sec
sorting block 29, #reads = 1093354 mem = 536880053 peak = 1078120448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290728, 10, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 614, 804)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2094)
[M::mem_pestat] mean and std.dev: (548.80, 390.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2739)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.16, 119.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1484, 2494, 2494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4514)
[M::mem_pestat] mean and std.dev: (1844.70, 737.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5524)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 697804 sequences (100000253 bp) in 2.107 CPU sec, 2.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290538, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.36, 118.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (303, 310, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (346.40, 165.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1347)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698066 reads in 132.049 CPU sec, 4.207 real sec
[M::process] read 697618 sequences (100000135 bp) in 1.514 CPU sec, 1.528 real sec...
sorting block 30, #reads = 1093601 mem = 536883849 peak = 1078120448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290635, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.93, 120.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697518 sequences (100000209 bp) in 1.617 CPU sec, 1.617 real sec...
[M::mem_process_seqs] Processed 697804 reads in 77.994 CPU sec, 2.665 real sec
sorting block 31, #reads = 1091733 mem = 536886809 peak = 1078120448
[M::mem_process_seqs] Processed 697494 reads in 189.729 CPU sec, 6.206 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290807, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.61, 120.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701640 sequences (100000061 bp) in 2.021 CPU sec, 2.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291277, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.73, 120.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697618 reads in 144.347 CPU sec, 4.588 real sec
[M::mem_process_seqs] Processed 697518 reads in 84.841 CPU sec, 2.679 real sec
sorting block 32, #reads = 1091895 mem = 536877072 peak = 1078120448
[M::process] read 703084 sequences (100000205 bp) in 1.750 CPU sec, 1.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291940, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (243.86, 117.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1091543 mem = 536888259 peak = 1078120448
[M::process] read 702492 sequences (100000179 bp) in 2.142 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292194, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (241.90, 116.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701640 reads in 132.831 CPU sec, 4.226 real sec
[M::process] read 701642 sequences (100000003 bp) in 1.522 CPU sec, 1.535 real sec...
[M::mem_process_seqs] Processed 703084 reads in 83.549 CPU sec, 2.637 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292195, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (242.88, 117.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 252, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (272.50, 86.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RR
sorting block 34, #reads = 1094952 mem = 536879800 peak = 1078120448
[M::process] read 701510 sequences (100000124 bp) in 2.263 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292088, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.72, 117.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 203, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208)
[M::mem_pestat] mean and std.dev: (268.78, 138.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1550)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702492 reads in 129.998 CPU sec, 4.158 real sec
[M::mem_process_seqs] Processed 701642 reads in 83.490 CPU sec, 2.638 real sec
[M::process] read 700520 sequences (100000265 bp) in 1.970 CPU sec, 2.074 real sec...
sorting block 35, #reads = 1097416 mem = 536882541 peak = 1078120448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292103, 9, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.15, 119.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 233, 764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832)
[M::mem_pestat] mean and std.dev: (478.07, 417.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2366)
[M::mem_pestat] skip orientation RR
[M::process] read 699872 sequences (100000088 bp) in 2.182 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 292031, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 205, 797)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2041)
[M::mem_pestat] mean and std.dev: (421.69, 353.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2663)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (248.59, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 339, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (252.53, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701510 reads in 132.515 CPU sec, 4.229 real sec
[M::mem_process_seqs] Processed 700520 reads in 84.484 CPU sec, 2.665 real sec
sorting block 36, #reads = 1097694 mem = 536879537 peak = 1078120448
[M::process] read 699368 sequences (100000071 bp) in 1.849 CPU sec, 1.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291474, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.46, 120.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699330 sequences (100000066 bp) in 2.023 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291266, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.63, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 302, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512)
[M::mem_pestat] mean and std.dev: (230.00, 64.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699872 reads in 134.689 CPU sec, 4.295 real sec
sorting block 37, #reads = 1096154 mem = 536878476 peak = 1078120448
[M::mem_process_seqs] Processed 699368 reads in 86.009 CPU sec, 2.718 real sec
[M::process] read 700452 sequences (100000093 bp) in 1.695 CPU sec, 1.749 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290892, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.75, 121.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1094560 mem = 536876704 peak = 1098715136
[M::process] read 699498 sequences (100000138 bp) in 2.449 CPU sec, 2.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291910, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.26, 117.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699330 reads in 136.022 CPU sec, 4.334 real sec
[M::mem_process_seqs] Processed 700452 reads in 86.613 CPU sec, 2.734 real sec
[M::process] read 698442 sequences (100000170 bp) in 1.748 CPU sec, 1.794 real sec...
sorting block 39, #reads = 1094375 mem = 536876639 peak = 1098715136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291273, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (246.43, 117.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 431, 1146)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3056)
[M::mem_pestat] mean and std.dev: (520.64, 431.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4011)
[M::mem_pestat] skip orientation RR
[M::process] read 698154 sequences (100000063 bp) in 2.037 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290988, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (247.40, 117.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699498 reads in 135.719 CPU sec, 4.324 real sec
[M::process] read 698178 sequences (100000003 bp) in 1.559 CPU sec, 1.586 real sec...
[M::mem_process_seqs] Processed 698442 reads in 85.428 CPU sec, 2.695 real sec
sorting block 40, #reads = 1094234 mem = 536883426 peak = 1098715136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290398, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (247.93, 116.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697200 sequences (100000089 bp) in 2.261 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290030, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (247.79, 116.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697374 sequences (100000002 bp) in 1.388 CPU sec, 1.385 real sec...
[M::mem_process_seqs] Processed 698154 reads in 132.204 CPU sec, 4.201 real sec
sorting block 41, #reads = 1092939 mem = 536877692 peak = 1098715136
[M::mem_process_seqs] Processed 698178 reads in 84.279 CPU sec, 2.661 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290054, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 213, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (181.80, 68.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (248.44, 115.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 295, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (231.50, 103.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 42, #reads = 1092540 mem = 536879266 peak = 1098715136
[M::process] read 697380 sequences (100000072 bp) in 2.094 CPU sec, 2.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290285, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.58, 118.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697200 reads in 132.621 CPU sec, 4.234 real sec
[M::mem_process_seqs] Processed 697374 reads in 84.758 CPU sec, 2.675 real sec
[M::process] read 697010 sequences (100000131 bp) in 1.668 CPU sec, 1.685 real sec...
sorting block 43, #reads = 1091436 mem = 536879120 peak = 1098715136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290471, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.06, 119.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708512 sequences (100000198 bp) in 2.559 CPU sec, 2.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290745, 11, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.49, 121.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 798, 1087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2823)
[M::mem_pestat] mean and std.dev: (754.45, 673.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3691)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 697380 reads in 133.110 CPU sec, 4.236 real sec
[M::mem_process_seqs] Processed 697010 reads in 84.974 CPU sec, 2.684 real sec
[M::process] read 706434 sequences (100000229 bp) in 1.764 CPU sec, 1.824 real sec...
sorting block 44, #reads = 1091849 mem = 536876854 peak = 1098715136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 294221, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568)
[M::mem_pestat] mean and std.dev: (223.20, 104.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704252 sequences (100000203 bp) in 1.877 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293616, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (230.64, 110.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703222 sequences (100000294 bp) in 1.400 CPU sec, 1.392 real sec...
[M::mem_process_seqs] Processed 708512 reads in 140.151 CPU sec, 4.471 real sec
[M::mem_process_seqs] Processed 706434 reads in 87.093 CPU sec, 2.764 real sec
sorting block 45, #reads = 1092977 mem = 536876979 peak = 1098715136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293140, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.24, 114.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701920 sequences (100000130 bp) in 2.288 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292632, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (240.30, 117.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704252 reads in 133.161 CPU sec, 4.248 real sec
sorting block 46, #reads = 1096275 mem = 536882105 peak = 1098715136
[M::mem_process_seqs] Processed 703222 reads in 85.223 CPU sec, 2.693 real sec
[M::process] read 701700 sequences (100000216 bp) in 1.685 CPU sec, 1.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292272, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 170, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434)
[M::mem_pestat] mean and std.dev: (187.78, 75.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.62, 118.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 47, #reads = 1097189 mem = 536878589 peak = 1102512128
[M::process] read 700632 sequences (100000060 bp) in 1.975 CPU sec, 2.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292015, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.85, 118.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701920 reads in 133.877 CPU sec, 4.265 real sec
[M::process] read 700226 sequences (100000019 bp) in 1.454 CPU sec, 1.462 real sec...
[M::mem_process_seqs] Processed 701700 reads in 85.122 CPU sec, 2.688 real sec
sorting block 48, #reads = 1094773 mem = 536878577 peak = 1102512128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291917, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.04, 120.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699428 sequences (100000039 bp) in 2.350 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291559, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (247.12, 120.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700632 reads in 135.940 CPU sec, 4.320 real sec
[M::process] read 699044 sequences (100000167 bp) in 1.440 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 700226 reads in 86.647 CPU sec, 2.739 real sec
sorting block 49, #reads = 1093956 mem = 536881146 peak = 1102512128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291391, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.55, 120.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699008 sequences (100000195 bp) in 2.018 CPU sec, 2.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291004, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.51, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699428 reads in 136.247 CPU sec, 4.341 real sec
[M::process] read 698758 sequences (100000161 bp) in 1.420 CPU sec, 1.421 real sec...
sorting block 50, #reads = 1092776 mem = 536879815 peak = 1102512128
[M::mem_process_seqs] Processed 699044 reads in 85.875 CPU sec, 2.719 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291234, 7, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.19, 119.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 357, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (340.15, 152.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RR
sorting block 51, #reads = 1092378 mem = 536885494 peak = 1114189824
[M::process] read 698552 sequences (100000110 bp) in 2.300 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291247, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 462, 2048)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5720)
[M::mem_pestat] mean and std.dev: (1484.14, 1824.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.79, 119.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698170 sequences (100000096 bp) in 1.420 CPU sec, 1.416 real sec...
[M::mem_process_seqs] Processed 699008 reads in 137.566 CPU sec, 4.369 real sec
[M::mem_process_seqs] Processed 698758 reads in 87.472 CPU sec, 2.770 real sec
sorting block 52, #reads = 1091375 mem = 536879611 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291217, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.82, 119.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698372 sequences (100000283 bp) in 2.320 CPU sec, 2.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290890, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.47, 119.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 280, 909)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363)
[M::mem_pestat] mean and std.dev: (498.10, 480.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3090)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698552 reads in 141.854 CPU sec, 4.515 real sec
[M::process] read 698122 sequences (100000020 bp) in 1.565 CPU sec, 1.581 real sec...
[M::mem_process_seqs] Processed 698170 reads in 89.727 CPU sec, 2.835 real sec
sorting block 53, #reads = 1091434 mem = 536876815 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290554, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 215, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (218.80, 73.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.49, 119.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698660 sequences (100000153 bp) in 2.326 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290992, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 221, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (205.89, 88.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.17, 120.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 514, 594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336)
[M::mem_pestat] mean and std.dev: (449.85, 262.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1707)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698372 reads in 142.546 CPU sec, 4.537 real sec
[M::process] read 710872 sequences (100000175 bp) in 1.655 CPU sec, 1.663 real sec...
[M::mem_process_seqs] Processed 698122 reads in 89.706 CPU sec, 2.828 real sec
sorting block 54, #reads = 1090689 mem = 536877161 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291136, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (261, 414, 704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1590)
[M::mem_pestat] mean and std.dev: (553.60, 420.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2237)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.65, 119.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 709374 sequences (100000101 bp) in 2.402 CPU sec, 2.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 294741, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 197, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (218.92, 102.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698660 reads in 148.167 CPU sec, 4.729 real sec
sorting block 55, #reads = 1090750 mem = 536878075 peak = 1114189824
[M::mem_process_seqs] Processed 710872 reads in 95.174 CPU sec, 3.005 real sec
[M::process] read 707204 sequences (100000300 bp) in 1.771 CPU sec, 1.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 294343, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 200, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (222.23, 104.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1092825 mem = 536883430 peak = 1114189824
[M::process] read 705682 sequences (100000219 bp) in 2.187 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293805, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (228.15, 108.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705264 sequences (100000121 bp) in 1.470 CPU sec, 1.471 real sec...
[M::mem_process_seqs] Processed 709374 reads in 149.745 CPU sec, 4.771 real sec
[M::mem_process_seqs] Processed 707204 reads in 92.546 CPU sec, 2.929 real sec
sorting block 57, #reads = 1093613 mem = 536879812 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293325, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (232.25, 111.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704446 sequences (100000137 bp) in 2.245 CPU sec, 2.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293348, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.13, 112.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705682 reads in 143.134 CPU sec, 4.555 real sec
[M::process] read 703820 sequences (100000218 bp) in 1.482 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 705264 reads in 90.544 CPU sec, 2.866 real sec
sorting block 58, #reads = 1094407 mem = 536881058 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 293121, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.42, 114.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702216 sequences (100000261 bp) in 2.370 CPU sec, 2.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293126, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (238.75, 115.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 197, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382)
[M::mem_pestat] mean and std.dev: (246.50, 169.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1795)
[M::mem_pestat] skip orientation RR
[M::process] read 701080 sequences (100000121 bp) in 1.489 CPU sec, 1.492 real sec...
[M::mem_process_seqs] Processed 704446 reads in 139.847 CPU sec, 4.439 real sec
sorting block 59, #reads = 1092833 mem = 536881259 peak = 1114189824
[M::mem_process_seqs] Processed 703820 reads in 88.698 CPU sec, 2.806 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292226, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 190, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (155.78, 52.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.91, 118.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (324, 547, 785)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1707)
[M::mem_pestat] mean and std.dev: (479.12, 167.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2168)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 60, #reads = 1093486 mem = 536879044 peak = 1114189824
[M::process] read 699984 sequences (100000032 bp) in 2.261 CPU sec, 2.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292162, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (245.14, 118.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702216 reads in 139.497 CPU sec, 4.444 real sec
[M::process] read 699622 sequences (100000181 bp) in 1.492 CPU sec, 1.506 real sec...
[M::mem_process_seqs] Processed 701080 reads in 89.475 CPU sec, 2.824 real sec
sorting block 61, #reads = 1092130 mem = 536877390 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291285, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 552, 959)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2453)
[M::mem_pestat] mean and std.dev: (480.50, 313.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3200)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.50, 119.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699166 sequences (100000141 bp) in 2.373 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291851, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.01, 118.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 426, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 668)
[M::mem_pestat] mean and std.dev: (313.25, 105.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RR
[M::process] read 699444 sequences (100000190 bp) in 1.419 CPU sec, 1.420 real sec...
[M::mem_process_seqs] Processed 699984 reads in 138.873 CPU sec, 4.411 real sec
[M::mem_process_seqs] Processed 699622 reads in 87.464 CPU sec, 2.766 real sec
sorting block 62, #reads = 1091849 mem = 536878549 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291554, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.88, 119.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698284 sequences (100000101 bp) in 2.055 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291237, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 209, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (230.78, 133.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1366)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.78, 118.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697962 sequences (100000047 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 699166 reads in 136.681 CPU sec, 4.352 real sec
[M::mem_process_seqs] Processed 699444 reads in 87.143 CPU sec, 2.756 real sec
sorting block 63, #reads = 1091406 mem = 536879816 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290834, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 199, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (201.67, 78.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.84, 120.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698676 sequences (100000151 bp) in 2.449 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290924, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.19, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698284 reads in 134.943 CPU sec, 4.287 real sec
sorting block 64, #reads = 1092212 mem = 536882824 peak = 1114189824
[M::process] read 707150 sequences (100000197 bp) in 1.569 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290650, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.96, 119.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 748, 2241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6293)
[M::mem_pestat] mean and std.dev: (1077.10, 963.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8319)
[M::mem_pestat] skip orientation RR
[M::process] read 707500 sequences (100000015 bp) in 1.573 CPU sec, 1.582 real sec...
[M::mem_process_seqs] Processed 698676 reads in 84.467 CPU sec, 2.918 real sec
sorting block 65, #reads = 1090876 mem = 536877386 peak = 1114189824
[M::mem_process_seqs] Processed 697962 reads in 191.733 CPU sec, 6.320 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 294212, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (228.22, 108.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705936 sequences (100000065 bp) in 2.374 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 294006, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (228.36, 108.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707150 reads in 153.593 CPU sec, 4.888 real sec
sorting block 66, #reads = 1089642 mem = 536880701 peak = 1114189824
[M::mem_process_seqs] Processed 707500 reads in 87.165 CPU sec, 2.753 real sec
[M::process] read 704508 sequences (100000095 bp) in 1.851 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293559, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 208, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (232.36, 111.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1096612 mem = 536879698 peak = 1114189824
[M::process] read 703916 sequences (100000130 bp) in 2.310 CPU sec, 2.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293229, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.18, 113.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 335, 652)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626)
[M::mem_pestat] mean and std.dev: (352.44, 280.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2113)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 705936 reads in 133.706 CPU sec, 4.257 real sec
[M::mem_process_seqs] Processed 704508 reads in 84.457 CPU sec, 2.668 real sec
[M::process] read 702888 sequences (100000223 bp) in 1.762 CPU sec, 1.800 real sec...
sorting block 68, #reads = 1098426 mem = 536878055 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292854, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.42, 114.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702840 sequences (100000175 bp) in 2.399 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292532, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.56, 116.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703916 reads in 140.550 CPU sec, 4.471 real sec
[M::mem_process_seqs] Processed 702888 reads in 91.855 CPU sec, 2.897 real sec
[M::process] read 702538 sequences (100000158 bp) in 1.651 CPU sec, 1.678 real sec...
sorting block 69, #reads = 1096630 mem = 536880203 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292939, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.68, 115.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701578 sequences (100000070 bp) in 2.258 CPU sec, 2.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292433, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (241.30, 116.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702840 reads in 138.808 CPU sec, 4.427 real sec
[M::process] read 701742 sequences (100000200 bp) in 1.557 CPU sec, 1.580 real sec...
[M::mem_process_seqs] Processed 702538 reads in 87.655 CPU sec, 2.762 real sec
sorting block 70, #reads = 1094890 mem = 536876771 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291729, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 200, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429)
[M::mem_pestat] mean and std.dev: (202.08, 57.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (243.86, 117.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 71, #reads = 1095554 mem = 536882369 peak = 1114189824
[M::process] read 700132 sequences (100000029 bp) in 2.398 CPU sec, 2.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291962, 8, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.56, 115.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 298, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (266.54, 179.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RR
[M::process] read 699620 sequences (100000184 bp) in 1.460 CPU sec, 1.466 real sec...
[M::mem_process_seqs] Processed 701578 reads in 140.168 CPU sec, 4.453 real sec
[M::mem_process_seqs] Processed 701742 reads in 89.261 CPU sec, 2.822 real sec
sorting block 72, #reads = 1093624 mem = 536877096 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291645, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.29, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699644 sequences (100000108 bp) in 2.328 CPU sec, 2.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291469, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (248.05, 119.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699554 sequences (100000061 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 700132 reads in 137.693 CPU sec, 4.374 real sec
[M::mem_process_seqs] Processed 699620 reads in 87.436 CPU sec, 2.769 real sec
sorting block 73, #reads = 1092562 mem = 536878889 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291338, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.14, 118.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 199, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (290.42, 144.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1347)
[M::mem_pestat] skip orientation RR
[M::process] read 698864 sequences (100000012 bp) in 2.301 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291809, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 287, 1557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4257)
[M::mem_pestat] mean and std.dev: (689.00, 598.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.17, 117.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698642 sequences (100000201 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_process_seqs] Processed 699644 reads in 139.571 CPU sec, 4.432 real sec
[M::mem_process_seqs] Processed 699554 reads in 88.169 CPU sec, 2.791 real sec
sorting block 74, #reads = 1091742 mem = 536881520 peak = 1114189824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290976, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 354, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (356.09, 186.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.44, 118.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698580 sequences (100000173 bp) in 2.396 CPU sec, 2.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290814, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.20, 119.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 711410 sequences (100000026 bp) in 1.470 CPU sec, 1.472 real sec...
[M::mem_process_seqs] Processed 698864 reads in 141.296 CPU sec, 4.485 real sec
sorting block 75, #reads = 1090941 mem = 536880990 peak = 1114189824
[M::process] read 709862 sequences (100000050 bp) in 1.703 CPU sec, 1.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290447, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 319, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (256.25, 103.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.57, 118.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 375, 1383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3809)
[M::mem_pestat] mean and std.dev: (506.07, 596.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5022)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698580 reads in 90.634 CPU sec, 3.129 real sec
sorting block 76, #reads = 1088495 mem = 536880146 peak = 1114189824
[M::mem_process_seqs] Processed 698642 reads in 202.047 CPU sec, 6.665 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 295213, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 167, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (201.83, 97.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 196, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (218.30, 102.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 691)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 493, 991)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2681)
[M::mem_pestat] mean and std.dev: (421.62, 346.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3526)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 708128 sequences (100000080 bp) in 2.301 CPU sec, 2.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 294864, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 200, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (223.38, 105.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 716)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 711410 reads in 164.156 CPU sec, 5.215 real sec
[M::process] read 706674 sequences (100000140 bp) in 1.603 CPU sec, 1.611 real sec...
sorting block 77, #reads = 1085021 mem = 536879591 peak = 1114189824
[M::mem_process_seqs] Processed 709862 reads in 94.110 CPU sec, 2.973 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 294069, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (227.91, 108.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1095615 mem = 536881715 peak = 1134493696
[M::process] read 705234 sequences (100000134 bp) in 2.254 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293284, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (232.02, 111.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 255, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (72, 487)
[M::mem_pestat] mean and std.dev: (240.09, 46.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] skip orientation RR
[M::process] read 703712 sequences (100000287 bp) in 1.480 CPU sec, 1.482 real sec...
[M::mem_process_seqs] Processed 708128 reads in 146.588 CPU sec, 4.661 real sec
[M::mem_process_seqs] Processed 706674 reads in 92.887 CPU sec, 2.945 real sec
sorting block 79, #reads = 1093113 mem = 536882530 peak = 1134493696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293735, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (235.34, 113.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703894 sequences (100000255 bp) in 2.372 CPU sec, 2.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292548, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.04, 115.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703090 sequences (100000173 bp) in 1.450 CPU sec, 1.457 real sec...
[M::mem_process_seqs] Processed 705234 reads in 143.279 CPU sec, 4.554 real sec
[M::mem_process_seqs] Processed 703712 reads in 90.168 CPU sec, 2.860 real sec
sorting block 80, #reads = 1093655 mem = 536877815 peak = 1134493696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292584, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 263, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (274.20, 149.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1351)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (239.22, 116.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 361, 1219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3203)
[M::mem_pestat] mean and std.dev: (607.00, 545.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4195)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 702730 sequences (100000074 bp) in 2.173 CPU sec, 2.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292735, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.77, 116.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700092 sequences (100000180 bp) in 1.450 CPU sec, 1.442 real sec...
[M::mem_process_seqs] Processed 703894 reads in 145.129 CPU sec, 4.625 real sec
sorting block 81, #reads = 1092455 mem = 536877739 peak = 1134493696
[M::mem_process_seqs] Processed 703090 reads in 90.501 CPU sec, 2.865 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292996, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (238.61, 114.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 82, #reads = 1091967 mem = 536879053 peak = 1134493696
[M::process] read 699360 sequences (100000020 bp) in 2.426 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291789, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (245.48, 117.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699236 sequences (100000196 bp) in 1.440 CPU sec, 1.434 real sec...
[M::mem_process_seqs] Processed 702730 reads in 142.336 CPU sec, 4.522 real sec
[M::mem_process_seqs] Processed 700092 reads in 89.774 CPU sec, 2.838 real sec
sorting block 83, #reads = 1091419 mem = 536878182 peak = 1134493696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291641, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.05, 116.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698268 sequences (100000056 bp) in 2.037 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290551, 11, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (245.32, 115.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (319, 928, 1607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4183)
[M::mem_pestat] mean and std.dev: (841.27, 566.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5471)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 697866 sequences (100000178 bp) in 1.410 CPU sec, 1.404 real sec...
[M::mem_process_seqs] Processed 699360 reads in 138.276 CPU sec, 4.402 real sec
[M::mem_process_seqs] Processed 699236 reads in 87.390 CPU sec, 2.766 real sec
sorting block 84, #reads = 1091950 mem = 536881979 peak = 1134493696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290510, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (246.54, 115.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 907, 1111)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2837)
[M::mem_pestat] mean and std.dev: (638.20, 408.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3700)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 698056 sequences (100000200 bp) in 2.275 CPU sec, 2.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290066, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (247.84, 116.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697234 sequences (100000109 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 698268 reads in 137.761 CPU sec, 4.380 real sec
[M::mem_process_seqs] Processed 697866 reads in 86.924 CPU sec, 2.751 real sec
sorting block 85, #reads = 1090789 mem = 536882093 peak = 1134493696
[M::process] read 274784 sequences (39390237 bp) in 1.120 CPU sec, 1.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290386, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.09, 119.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290021, 11, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.85, 120.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 2007, 9737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28711)
[M::mem_pestat] mean and std.dev: (4708.91, 4616.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38198)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698056 reads in 133.435 CPU sec, 4.322 real sec
sorting block 86, #reads = 1089804 mem = 536877461 peak = 1134493696
[M::mem_process_seqs] Processed 697234 reads in 83.679 CPU sec, 2.713 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 114295, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.53, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 274784 reads in 32.421 CPU sec, 1.096 real sec
sorting block 87, #reads = 1090727 mem = 536878940 peak = 1167138816
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96270018
overall: 70047121408 mem 11527.770 user 400.994 sys 396.649 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 396.695 sec; CPU: 11928.766 sec
sorting block 88, #reads = 537116 mem = 264824847 peak = 1167138816
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5227667440
output reads: 96761901
bam_mem_sort: 89 calls 34.968 user 0.571 sys 35.747 real
bam_write: 89 calls 45.517 user 1.781 sys 47.722 real
execute: 1 calls 19.452 user 1.179 sys 424.438 real
merge_files: 1 calls 19.432 user 1.160 sys 27.116 real
parse_chunk: 1416 calls 45.490 user 11.999 sys 58.419 real
read_chunk: 3825 calls 37.734 user 35.340 sys 342.245 real
sort_block: 1 calls 29.182 user 34.747 sys 329.989 real
write_chunk: 2407 calls 18.988 user 0.936 sys 26.198 real
overall: 3009306624 mem 1059.161 user 59.844 sys 424.461 real
ls: cannot access '*.cram': No such file or directory