Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8f/b1b231963451c25cc0c465e0660535/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f9/56c080a96ad0dc70970ce931f7dd1d/659_cAd-T1-TDNA-1_A23MHWWLT4_1/659_cAd-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8f/b1b231963451c25cc0c465e0660535/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f9/56c080a96ad0dc70970ce931f7dd1d/659_cAd-T1-TDNA-1_A23MHWWLT4_1/659_cAd-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cAd-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1742.499 CPU sec, 65.500 real sec
[M::process] read 687088 sequences (100000053 bp) in 0.978 CPU sec, 0.981 real sec...
[M::process] read 684912 sequences (100000148 bp) in 1.489 CPU sec, 1.490 real sec...
[M::process] read 684264 sequences (100000048 bp) in 1.103 CPU sec, 1.112 real sec...
[M::process] read 683806 sequences (100000281 bp) in 1.101 CPU sec, 1.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287077, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (307.74, 143.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684912 reads in 70.647 CPU sec, 2.266 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288651, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 279, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (296.57, 139.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683378 sequences (100000237 bp) in 1.219 CPU sec, 1.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287234, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (309.36, 142.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687088 reads in 212.040 CPU sec, 6.727 real sec
sorting block 0, #reads = 1087971 mem = 536879830 peak = 601927680
[M::process] read 683102 sequences (100000027 bp) in 1.250 CPU sec, 1.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287513, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (311.36, 143.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683806 reads in 71.066 CPU sec, 2.266 real sec
[M::mem_process_seqs] Processed 684264 reads in 178.369 CPU sec, 5.714 real sec
sorting block 1, #reads = 1087384 mem = 536883310 peak = 732336128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287596, 10, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 231, 981)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505)
[M::mem_pestat] mean and std.dev: (689.09, 605.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3267)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (312.84, 143.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 826, 826)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2020)
[M::mem_pestat] mean and std.dev: (599.30, 421.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2617)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 285, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (237.20, 90.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682808 sequences (100000244 bp) in 1.420 CPU sec, 1.487 real sec...
[M::process] read 682544 sequences (100000092 bp) in 1.170 CPU sec, 1.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287316, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (315.60, 144.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683378 reads in 142.967 CPU sec, 4.569 real sec
[M::mem_process_seqs] Processed 683102 reads in 81.721 CPU sec, 2.613 real sec
sorting block 2, #reads = 1086538 mem = 536879083 peak = 844492800
[M::process] read 682370 sequences (100000214 bp) in 1.541 CPU sec, 1.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287173, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (317.09, 145.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682082 sequences (100000148 bp) in 1.223 CPU sec, 1.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286998, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 306, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (320.15, 147.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682808 reads in 130.309 CPU sec, 4.155 real sec
sorting block 3, #reads = 1086020 mem = 536878626 peak = 844492800
[M::mem_process_seqs] Processed 682544 reads in 80.805 CPU sec, 2.583 real sec
sorting block 4, #reads = 1085927 mem = 536877971 peak = 844492800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287145, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 306, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (320.74, 147.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 1979, 1979)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5895)
[M::mem_pestat] mean and std.dev: (1305.77, 945.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7853)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 681940 sequences (100000196 bp) in 1.656 CPU sec, 1.791 real sec...
[M::process] read 681404 sequences (100000159 bp) in 1.200 CPU sec, 1.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287079, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (319.69, 146.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682370 reads in 129.472 CPU sec, 4.128 real sec
[M::mem_process_seqs] Processed 682082 reads in 80.613 CPU sec, 2.571 real sec
sorting block 5, #reads = 1086189 mem = 536880651 peak = 844492800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287207, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (322.45, 147.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (326, 358, 1628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4232)
[M::mem_pestat] mean and std.dev: (820.00, 657.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5534)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 681374 sequences (100000124 bp) in 1.818 CPU sec, 1.942 real sec...
[M::process] read 681424 sequences (100000122 bp) in 1.230 CPU sec, 1.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286552, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 309, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (323.31, 147.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681940 reads in 129.327 CPU sec, 4.118 real sec
[M::mem_process_seqs] Processed 681404 reads in 80.950 CPU sec, 2.571 real sec
sorting block 6, #reads = 1085380 mem = 536877132 peak = 844492800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287030, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 311, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (324.89, 148.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681430 sequences (100000145 bp) in 1.673 CPU sec, 1.800 real sec...
[M::process] read 681266 sequences (100000236 bp) in 1.280 CPU sec, 1.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286454, 16, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (324.05, 148.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 210, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (196, 231)
[M::mem_pestat] mean and std.dev: (210.58, 1.93)
[M::mem_pestat] low and high boundaries for proper pairs: (189, 238)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 681374 reads in 131.937 CPU sec, 4.214 real sec
[M::mem_process_seqs] Processed 681424 reads in 82.903 CPU sec, 2.631 real sec
sorting block 7, #reads = 1086065 mem = 536877801 peak = 844492800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286754, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 309, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (324.51, 148.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681194 sequences (100000125 bp) in 1.929 CPU sec, 2.046 real sec...
[M::process] read 681006 sequences (100000075 bp) in 1.282 CPU sec, 1.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286615, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 309, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (323.72, 147.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681430 reads in 132.772 CPU sec, 4.232 real sec
sorting block 8, #reads = 1085664 mem = 536880826 peak = 844492800
[M::mem_process_seqs] Processed 681266 reads in 82.694 CPU sec, 2.631 real sec
sorting block 9, #reads = 1084798 mem = 536879849 peak = 865312768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285802, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (320.92, 145.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681090 sequences (100000255 bp) in 1.738 CPU sec, 1.846 real sec...
[M::process] read 681296 sequences (100000057 bp) in 1.329 CPU sec, 1.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286287, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (320.49, 144.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681194 reads in 133.197 CPU sec, 4.250 real sec
[M::mem_process_seqs] Processed 681006 reads in 84.092 CPU sec, 2.674 real sec
sorting block 10, #reads = 1084128 mem = 536878387 peak = 865312768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286201, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 279, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324)
[M::mem_pestat] mean and std.dev: (236.00, 134.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1715)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (318.68, 143.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681444 sequences (100000034 bp) in 2.163 CPU sec, 2.264 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286571, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 681550 sequences (100000215 bp) in 1.336 CPU sec, 1.339 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (205, 303, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (316.42, 142.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681090 reads in 134.733 CPU sec, 4.288 real sec
[M::mem_process_seqs] Processed 681296 reads in 84.090 CPU sec, 2.673 real sec
sorting block 11, #reads = 1084185 mem = 536878641 peak = 865312768
[M::process] read 681360 sequences (100000038 bp) in 1.857 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286960, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 302, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (315.76, 141.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681330 sequences (100000135 bp) in 1.389 CPU sec, 1.388 real sec...
[M::mem_process_seqs] Processed 681550 reads in 72.849 CPU sec, 2.297 real sec
sorting block 12, #reads = 1084361 mem = 536881454 peak = 865312768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286842, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 302, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (315.80, 141.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286256, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 303, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (316.04, 141.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681444 reads in 206.350 CPU sec, 6.567 real sec
[M::process] read 681218 sequences (100000116 bp) in 1.448 CPU sec, 1.457 real sec...
[M::mem_process_seqs] Processed 681360 reads in 96.718 CPU sec, 3.090 real sec
sorting block 13, #reads = 1084524 mem = 536879310 peak = 865312768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286662, 0, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 242, 5802)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16962)
[M::mem_pestat] mean and std.dev: (1755.73, 2480.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22542)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (317.57, 142.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (476, 1684, 1684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4100)
[M::mem_pestat] mean and std.dev: (1284.90, 649.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5308)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681026 sequences (100000149 bp) in 2.315 CPU sec, 2.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286642, 0, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (317.56, 142.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 427, 1011)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2607)
[M::mem_pestat] mean and std.dev: (603.00, 462.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3405)
[M::mem_pestat] skip orientation RR
[M::process] read 680608 sequences (100000267 bp) in 1.310 CPU sec, 1.306 real sec...
[M::mem_process_seqs] Processed 681330 reads in 133.691 CPU sec, 4.249 real sec
[M::mem_process_seqs] Processed 681218 reads in 84.386 CPU sec, 2.683 real sec
sorting block 14, #reads = 1083876 mem = 536884665 peak = 865312768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286654, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (319.00, 143.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680396 sequences (100000054 bp) in 2.061 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286458, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (321.88, 143.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (529, 910, 1060)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2122)
[M::mem_pestat] mean and std.dev: (673.36, 346.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2653)
[M::mem_pestat] skip orientation RR
[M::process] read 682534 sequences (100000168 bp) in 1.380 CPU sec, 1.378 real sec...
[M::mem_process_seqs] Processed 681026 reads in 137.786 CPU sec, 4.389 real sec
sorting block 15, #reads = 1082686 mem = 536879156 peak = 865312768
[M::mem_process_seqs] Processed 680608 reads in 85.539 CPU sec, 2.712 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286179, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (323.90, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1083499 mem = 536878736 peak = 882040832
[M::process] read 684818 sequences (100000015 bp) in 2.213 CPU sec, 2.326 real sec...
[M::process] read 684754 sequences (100000152 bp) in 1.330 CPU sec, 1.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286652, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 301, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (315.21, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680396 reads in 137.324 CPU sec, 4.369 real sec
[M::mem_process_seqs] Processed 682534 reads in 87.737 CPU sec, 2.785 real sec
sorting block 17, #reads = 1081878 mem = 536877203 peak = 882040832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287786, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (307.55, 143.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683936 sequences (100000218 bp) in 2.270 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287388, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (308.88, 143.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683798 sequences (100000295 bp) in 1.484 CPU sec, 1.483 real sec...
[M::mem_process_seqs] Processed 684818 reads in 137.996 CPU sec, 4.409 real sec
[M::mem_process_seqs] Processed 684754 reads in 89.387 CPU sec, 2.860 real sec
sorting block 18, #reads = 1086461 mem = 536879397 peak = 882040832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287417, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (311.72, 144.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683430 sequences (100000050 bp) in 2.120 CPU sec, 2.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287328, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (313.47, 144.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683172 sequences (100000034 bp) in 1.370 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 683936 reads in 137.875 CPU sec, 4.399 real sec
[M::mem_process_seqs] Processed 683798 reads in 85.664 CPU sec, 2.718 real sec
sorting block 19, #reads = 1087612 mem = 536877443 peak = 882040832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287066, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (313.98, 144.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682762 sequences (100000044 bp) in 2.063 CPU sec, 2.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287673, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 303, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (317.10, 145.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 256, 1005)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2543)
[M::mem_pestat] mean and std.dev: (525.90, 407.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3312)
[M::mem_pestat] skip orientation RR
[M::process] read 682328 sequences (100000167 bp) in 1.424 CPU sec, 1.423 real sec...
[M::mem_process_seqs] Processed 683430 reads in 137.328 CPU sec, 4.384 real sec
sorting block 20, #reads = 1087624 mem = 536880040 peak = 882040832
[M::mem_process_seqs] Processed 683172 reads in 86.316 CPU sec, 2.739 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287562, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (319.57, 146.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1086032 mem = 536883798 peak = 926396416
[M::process] read 682190 sequences (100000102 bp) in 2.275 CPU sec, 2.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287690, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (320.57, 146.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682042 sequences (100000105 bp) in 1.480 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 682762 reads in 139.492 CPU sec, 4.445 real sec
[M::mem_process_seqs] Processed 682328 reads in 86.595 CPU sec, 2.754 real sec
sorting block 22, #reads = 1086299 mem = 536881819 peak = 926396416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286930, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (320.72, 146.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681656 sequences (100000135 bp) in 2.302 CPU sec, 2.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287022, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (321.29, 146.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681512 sequences (100000050 bp) in 1.388 CPU sec, 1.393 real sec...
[M::mem_process_seqs] Processed 682190 reads in 137.383 CPU sec, 4.371 real sec
[M::mem_process_seqs] Processed 682042 reads in 84.284 CPU sec, 2.671 real sec
sorting block 23, #reads = 1086447 mem = 536877772 peak = 926396416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286748, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (323.16, 146.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681594 sequences (100000142 bp) in 2.661 CPU sec, 2.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286736, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 312, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (325.27, 148.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681656 reads in 135.562 CPU sec, 4.298 real sec
[M::mem_process_seqs] Processed 681512 reads in 85.264 CPU sec, 2.681 real sec
[M::process] read 681396 sequences (100000241 bp) in 1.764 CPU sec, 1.808 real sec...
sorting block 24, #reads = 1086527 mem = 536880133 peak = 926396416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286908, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 311, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (324.81, 148.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 413, 1675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4571)
[M::mem_pestat] mean and std.dev: (821.45, 679.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6019)
[M::mem_pestat] skip orientation RR
[M::process] read 681134 sequences (100000266 bp) in 2.522 CPU sec, 2.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286776, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 311, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (324.83, 147.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681594 reads in 137.548 CPU sec, 4.368 real sec
[M::mem_process_seqs] Processed 681396 reads in 85.182 CPU sec, 2.678 real sec
sorting block 25, #reads = 1086136 mem = 536880595 peak = 926396416
[M::process] read 681002 sequences (100000267 bp) in 1.951 CPU sec, 2.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286824, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 1262, 1262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3414)
[M::mem_pestat] mean and std.dev: (719.88, 542.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 313, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (326.18, 148.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 26, #reads = 1085752 mem = 536877644 peak = 968421376
[M::process] read 681038 sequences (100000021 bp) in 2.133 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286600, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 313, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (326.47, 148.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681134 reads in 136.106 CPU sec, 4.327 real sec
[M::process] read 680834 sequences (100000131 bp) in 1.310 CPU sec, 1.307 real sec...
[M::mem_process_seqs] Processed 681002 reads in 86.361 CPU sec, 2.732 real sec
sorting block 27, #reads = 1085411 mem = 536878027 peak = 968421376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286497, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 305, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (317.73, 141.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680922 sequences (100000004 bp) in 2.196 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286892, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (321.31, 143.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681038 reads in 138.979 CPU sec, 4.400 real sec
[M::process] read 681014 sequences (100000274 bp) in 1.760 CPU sec, 1.758 real sec...
[M::mem_process_seqs] Processed 680834 reads in 87.502 CPU sec, 2.760 real sec
sorting block 28, #reads = 1083980 mem = 536882972 peak = 968421376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286640, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (249, 270, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 426)
[M::mem_pestat] mean and std.dev: (276.78, 40.38)
[M::mem_pestat] low and high boundaries for proper pairs: (72, 485)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (319.54, 142.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681302 sequences (100000272 bp) in 2.545 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287005, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (318.60, 142.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680922 reads in 135.113 CPU sec, 4.289 real sec
[M::mem_process_seqs] Processed 681014 reads in 84.899 CPU sec, 2.669 real sec
[M::process] read 681308 sequences (100000251 bp) in 2.025 CPU sec, 2.090 real sec...
sorting block 29, #reads = 1083898 mem = 536876427 peak = 968421376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286853, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 305, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (318.01, 142.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 364, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (303.90, 120.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] skip orientation RR
[M::process] read 681074 sequences (100000104 bp) in 2.242 CPU sec, 2.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286478, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 305, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (318.26, 142.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681302 reads in 136.325 CPU sec, 4.335 real sec
sorting block 30, #reads = 1084244 mem = 536882291 peak = 968421376
[M::mem_process_seqs] Processed 681308 reads in 86.049 CPU sec, 2.712 real sec
[M::process] read 680998 sequences (100000158 bp) in 1.655 CPU sec, 1.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286875, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (319.77, 144.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1085761 mem = 536879478 peak = 989843456
[M::process] read 681404 sequences (100000284 bp) in 2.187 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286100, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (318.67, 142.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681446 sequences (100000277 bp) in 1.320 CPU sec, 1.319 real sec...
[M::mem_process_seqs] Processed 681074 reads in 137.239 CPU sec, 4.364 real sec
[M::mem_process_seqs] Processed 680998 reads in 85.654 CPU sec, 2.709 real sec
sorting block 32, #reads = 1082817 mem = 536880826 peak = 989843456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286171, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 305, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (318.40, 143.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681024 sequences (100000062 bp) in 2.266 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286843, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (319.84, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680806 sequences (100000113 bp) in 1.549 CPU sec, 1.546 real sec...
[M::mem_process_seqs] Processed 681404 reads in 135.355 CPU sec, 4.289 real sec
[M::mem_process_seqs] Processed 681446 reads in 85.493 CPU sec, 2.698 real sec
sorting block 33, #reads = 1084629 mem = 536879232 peak = 989843456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286797, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (322.07, 145.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683152 sequences (100000158 bp) in 2.428 CPU sec, 2.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287172, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (324.19, 146.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685896 sequences (100000006 bp) in 1.349 CPU sec, 1.344 real sec...
[M::mem_process_seqs] Processed 681024 reads in 137.628 CPU sec, 4.365 real sec
sorting block 34, #reads = 1082930 mem = 536880361 peak = 989843456
[M::mem_process_seqs] Processed 680806 reads in 87.046 CPU sec, 2.755 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286870, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 224, 1167)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3053)
[M::mem_pestat] mean and std.dev: (607.45, 563.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3996)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (312.93, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685650 sequences (100000165 bp) in 2.464 CPU sec, 2.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287970, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (305.18, 142.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685248 sequences (100000140 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_process_seqs] Processed 683152 reads in 137.407 CPU sec, 4.357 real sec
sorting block 35, #reads = 1082050 mem = 536878114 peak = 989843456
[M::mem_process_seqs] Processed 685896 reads in 84.507 CPU sec, 2.670 real sec
sorting block 36, #reads = 1086308 mem = 536877689 peak = 989843456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287795, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (305.53, 142.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685006 sequences (100000138 bp) in 2.047 CPU sec, 2.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287583, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (306.35, 142.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684930 sequences (100000149 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 685650 reads in 134.112 CPU sec, 4.268 real sec
[M::mem_process_seqs] Processed 685248 reads in 83.576 CPU sec, 2.647 real sec
sorting block 37, #reads = 1087964 mem = 536879684 peak = 989843456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287381, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (308.72, 143.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684076 sequences (100000094 bp) in 2.505 CPU sec, 2.653 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288331, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (309.33, 144.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685006 reads in 134.218 CPU sec, 4.264 real sec
[M::process] read 683466 sequences (100000076 bp) in 1.472 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 684930 reads in 85.192 CPU sec, 2.692 real sec
sorting block 38, #reads = 1086904 mem = 536880800 peak = 989843456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287997, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (313.52, 145.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683570 sequences (100000184 bp) in 2.309 CPU sec, 2.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287965, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (316.00, 146.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 128, 5000)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14744)
[M::mem_pestat] mean and std.dev: (2180.27, 2876.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19616)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 682728 sequences (100000275 bp) in 1.330 CPU sec, 1.325 real sec...
[M::mem_process_seqs] Processed 684076 reads in 135.425 CPU sec, 4.304 real sec
sorting block 39, #reads = 1087065 mem = 536878264 peak = 989843456
[M::mem_process_seqs] Processed 683466 reads in 84.982 CPU sec, 2.692 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287927, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 301, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (315.51, 146.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1086009 mem = 536882210 peak = 989843456
[M::process] read 682628 sequences (100000098 bp) in 2.347 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287627, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (318.97, 147.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682304 sequences (100000133 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_process_seqs] Processed 683570 reads in 133.688 CPU sec, 4.249 real sec
[M::mem_process_seqs] Processed 682728 reads in 83.343 CPU sec, 2.633 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287464, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (105, 166, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (185.22, 107.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1385)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (318.44, 146.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 41, #reads = 1085708 mem = 536879833 peak = 989843456
[M::process] read 682212 sequences (100000163 bp) in 2.408 CPU sec, 2.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287828, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (321.71, 147.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682628 reads in 132.606 CPU sec, 4.212 real sec
[M::process] read 682196 sequences (100000231 bp) in 1.416 CPU sec, 1.428 real sec...
[M::mem_process_seqs] Processed 682304 reads in 83.945 CPU sec, 2.653 real sec
sorting block 42, #reads = 1087243 mem = 536878281 peak = 989843456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287299, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (322.97, 149.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681868 sequences (100000158 bp) in 2.300 CPU sec, 2.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286496, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 308, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (322.83, 149.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682002 sequences (100000065 bp) in 1.360 CPU sec, 1.362 real sec...
[M::mem_process_seqs] Processed 682212 reads in 134.147 CPU sec, 4.263 real sec
[M::mem_process_seqs] Processed 682196 reads in 84.450 CPU sec, 2.672 real sec
sorting block 43, #reads = 1087050 mem = 536878847 peak = 989843456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287286, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (323.64, 147.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682180 sequences (100000287 bp) in 2.316 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287140, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 311, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (325.17, 149.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682274 sequences (100000298 bp) in 1.320 CPU sec, 1.321 real sec...
[M::mem_process_seqs] Processed 681868 reads in 132.966 CPU sec, 4.221 real sec
sorting block 44, #reads = 1085351 mem = 536878027 peak = 989843456
[M::mem_process_seqs] Processed 682002 reads in 83.063 CPU sec, 2.626 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286567, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 301, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (315.69, 143.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1084989 mem = 536877540 peak = 1005690880
[M::process] read 682444 sequences (100000170 bp) in 2.206 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286889, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 300, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (315.23, 143.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681456 sequences (100000200 bp) in 1.370 CPU sec, 1.372 real sec...
[M::mem_process_seqs] Processed 682180 reads in 135.620 CPU sec, 4.315 real sec
[M::mem_process_seqs] Processed 682274 reads in 86.360 CPU sec, 2.731 real sec
sorting block 46, #reads = 1084912 mem = 536878668 peak = 1005690880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286745, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 300, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (315.92, 144.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681086 sequences (100000015 bp) in 2.285 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287250, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 309, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (323.12, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681102 sequences (100000282 bp) in 1.320 CPU sec, 1.320 real sec...
[M::mem_process_seqs] Processed 682444 reads in 132.895 CPU sec, 4.223 real sec
[M::mem_process_seqs] Processed 681456 reads in 83.194 CPU sec, 2.632 real sec
sorting block 47, #reads = 1084615 mem = 536878344 peak = 1005690880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286520, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (322.84, 146.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681064 sequences (100000000 bp) in 2.299 CPU sec, 2.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 286818, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (359, 659, 2886)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7940)
[M::mem_pestat] mean and std.dev: (1280.12, 1518.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10467)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 309, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (323.09, 146.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 291, 1393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3681)
[M::mem_pestat] mean and std.dev: (965.00, 1216.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5830)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681060 sequences (100000033 bp) in 1.343 CPU sec, 1.355 real sec...
[M::mem_process_seqs] Processed 681086 reads in 132.950 CPU sec, 4.222 real sec
[M::mem_process_seqs] Processed 681102 reads in 83.019 CPU sec, 2.625 real sec
sorting block 48, #reads = 1085023 mem = 536877930 peak = 1005690880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286025, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 309, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (323.15, 146.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (423, 423, 714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296)
[M::mem_pestat] mean and std.dev: (444.50, 158.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1587)
[M::mem_pestat] skip orientation RR
[M::process] read 681180 sequences (100000258 bp) in 2.203 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286298, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 309, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (323.44, 147.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681140 sequences (100000123 bp) in 1.320 CPU sec, 1.318 real sec...
[M::mem_process_seqs] Processed 681064 reads in 132.504 CPU sec, 4.210 real sec
sorting block 49, #reads = 1084356 mem = 536876719 peak = 1005690880
[M::mem_process_seqs] Processed 681060 reads in 83.447 CPU sec, 2.640 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286475, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (322.26, 146.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1083763 mem = 536877172 peak = 1021976576
[M::process] read 681734 sequences (100000175 bp) in 2.685 CPU sec, 2.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286695, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (322.31, 145.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681180 reads in 133.691 CPU sec, 4.246 real sec
[M::mem_process_seqs] Processed 681140 reads in 84.074 CPU sec, 2.650 real sec
[M::process] read 680682 sequences (100000056 bp) in 1.529 CPU sec, 1.565 real sec...
sorting block 51, #reads = 1083344 mem = 536877295 peak = 1021976576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286705, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 308, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (296.60, 106.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 768)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 305, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (319.81, 146.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681648 sequences (100000262 bp) in 2.207 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286514, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 314, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (327.13, 148.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685568 sequences (100000052 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_process_seqs] Processed 681734 reads in 135.711 CPU sec, 4.314 real sec
[M::mem_process_seqs] Processed 680682 reads in 85.501 CPU sec, 2.708 real sec
sorting block 52, #reads = 1083422 mem = 536884192 peak = 1021976576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286970, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 350, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (311.10, 102.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (321.40, 146.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685368 sequences (100000008 bp) in 2.461 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287666, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (306.79, 144.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681648 reads in 139.299 CPU sec, 4.416 real sec
[M::process] read 684960 sequences (100000113 bp) in 1.735 CPU sec, 1.732 real sec...
[M::mem_process_seqs] Processed 685568 reads in 87.415 CPU sec, 2.753 real sec
sorting block 53, #reads = 1082936 mem = 536876682 peak = 1021976576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287489, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (305.39, 142.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684606 sequences (100000066 bp) in 2.526 CPU sec, 2.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287127, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (306.83, 142.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685368 reads in 136.241 CPU sec, 4.324 real sec
sorting block 54, #reads = 1087508 mem = 536880570 peak = 1021976576
[M::mem_process_seqs] Processed 684960 reads in 86.793 CPU sec, 2.734 real sec
[M::process] read 684362 sequences (100000015 bp) in 1.820 CPU sec, 1.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287653, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (308.12, 143.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1088193 mem = 536875926 peak = 1021976576
[M::process] read 684996 sequences (100000003 bp) in 2.169 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287372, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 154, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (193.50, 79.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 293, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (309.91, 144.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 684606 reads in 133.913 CPU sec, 4.261 real sec
[M::process] read 684018 sequences (100000175 bp) in 1.360 CPU sec, 1.360 real sec...
[M::mem_process_seqs] Processed 684362 reads in 84.776 CPU sec, 2.674 real sec
sorting block 56, #reads = 1088207 mem = 536883930 peak = 1021976576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287714, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (308.90, 145.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683652 sequences (100000221 bp) in 2.471 CPU sec, 2.603 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287599, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (422, 881, 4672)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13172)
[M::mem_pestat] mean and std.dev: (1552.17, 1821.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17422)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (312.75, 145.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 684996 reads in 133.847 CPU sec, 4.252 real sec
[M::process] read 683872 sequences (100000068 bp) in 1.422 CPU sec, 1.433 real sec...
[M::mem_process_seqs] Processed 684018 reads in 84.055 CPU sec, 2.650 real sec
sorting block 57, #reads = 1086143 mem = 536886873 peak = 1021976576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287195, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (313.65, 145.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683402 sequences (100000007 bp) in 2.568 CPU sec, 2.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287344, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (313.80, 145.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 252, 947)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2343)
[M::mem_pestat] mean and std.dev: (497.69, 392.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3041)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683652 reads in 133.270 CPU sec, 4.232 real sec
[M::mem_process_seqs] Processed 683872 reads in 84.511 CPU sec, 2.657 real sec
sorting block 58, #reads = 1087313 mem = 536877534 peak = 1021976576
[M::process] read 683048 sequences (100000132 bp) in 2.033 CPU sec, 2.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287137, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (316.64, 147.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682608 sequences (100000106 bp) in 2.177 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287356, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 303, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (318.47, 147.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683402 reads in 137.354 CPU sec, 4.370 real sec
sorting block 59, #reads = 1087512 mem = 536879881 peak = 1021976576
[M::process] read 682444 sequences (100000081 bp) in 1.442 CPU sec, 1.454 real sec...
[M::mem_process_seqs] Processed 683048 reads in 87.863 CPU sec, 2.771 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287537, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (319.85, 148.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1086948 mem = 536878567 peak = 1091612672
[M::process] read 682170 sequences (100000192 bp) in 2.635 CPU sec, 2.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286752, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (321.83, 149.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682608 reads in 136.379 CPU sec, 4.327 real sec
[M::mem_process_seqs] Processed 682444 reads in 86.758 CPU sec, 2.728 real sec
[M::process] read 682046 sequences (100000254 bp) in 1.798 CPU sec, 1.861 real sec...
sorting block 61, #reads = 1086969 mem = 536877756 peak = 1091612672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287046, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (322.58, 149.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681856 sequences (100000258 bp) in 2.418 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286671, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 309, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (324.12, 150.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682170 reads in 136.608 CPU sec, 4.339 real sec
[M::mem_process_seqs] Processed 682046 reads in 85.845 CPU sec, 2.705 real sec
[M::process] read 681986 sequences (100000272 bp) in 1.971 CPU sec, 2.020 real sec...
sorting block 62, #reads = 1085756 mem = 536878451 peak = 1091612672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286499, 5, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 827, 1094)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2894)
[M::mem_pestat] mean and std.dev: (610.15, 443.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3794)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (323.21, 148.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681436 sequences (100000164 bp) in 2.462 CPU sec, 2.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286985, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (323.13, 148.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681856 reads in 135.700 CPU sec, 4.312 real sec
[M::mem_process_seqs] Processed 681986 reads in 85.472 CPU sec, 2.692 real sec
sorting block 63, #reads = 1085910 mem = 536879511 peak = 1091612672
[M::process] read 681246 sequences (100000155 bp) in 1.944 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287033, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 309, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (322.26, 146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 64, #reads = 1085589 mem = 536878052 peak = 1091612672
[M::process] read 680780 sequences (100000103 bp) in 2.269 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286791, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 311, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (323.86, 146.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681436 reads in 143.305 CPU sec, 4.552 real sec
[M::process] read 680944 sequences (100000201 bp) in 1.627 CPU sec, 1.644 real sec...
[M::mem_process_seqs] Processed 681246 reads in 95.377 CPU sec, 3.007 real sec
sorting block 65, #reads = 1085845 mem = 536879521 peak = 1091612672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286663, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (325.70, 146.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680580 sequences (100000197 bp) in 2.756 CPU sec, 2.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286698, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (324.64, 146.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (329, 2308, 2308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6266)
[M::mem_pestat] mean and std.dev: (1683.36, 1243.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8245)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680780 reads in 161.549 CPU sec, 5.137 real sec
[M::process] read 680480 sequences (100000151 bp) in 1.760 CPU sec, 1.770 real sec...
[M::mem_process_seqs] Processed 680944 reads in 103.974 CPU sec, 3.288 real sec
sorting block 66, #reads = 1084561 mem = 536879021 peak = 1091612672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286144, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (325.02, 145.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680438 sequences (100000112 bp) in 2.370 CPU sec, 2.515 real sec...
[M::process] read 680370 sequences (100000214 bp) in 1.560 CPU sec, 1.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286516, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (326.33, 146.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680580 reads in 164.505 CPU sec, 5.250 real sec
[M::mem_process_seqs] Processed 680480 reads in 106.646 CPU sec, 3.387 real sec
sorting block 67, #reads = 1082902 mem = 536879814 peak = 1091612672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286642, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (325.11, 145.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680728 sequences (100000216 bp) in 2.682 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286235, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (323.95, 144.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680544 sequences (100000193 bp) in 1.894 CPU sec, 1.900 real sec...
[M::mem_process_seqs] Processed 680438 reads in 168.497 CPU sec, 5.397 real sec
sorting block 68, #reads = 1083985 mem = 536879837 peak = 1091612672
[M::mem_process_seqs] Processed 680370 reads in 109.766 CPU sec, 3.502 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286628, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (321.91, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1084183 mem = 536881512 peak = 1091612672
[M::process] read 680384 sequences (100000008 bp) in 2.692 CPU sec, 2.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286843, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (322.36, 143.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680728 reads in 170.530 CPU sec, 5.521 real sec
[M::process] read 683560 sequences (100000154 bp) in 1.772 CPU sec, 2.111 real sec...
[M::mem_process_seqs] Processed 680544 reads in 109.920 CPU sec, 3.569 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286501, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (288, 288, 1490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3894)
[M::mem_pestat] mean and std.dev: (726.25, 577.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5096)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 310, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (323.20, 144.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (329, 525, 983)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2291)
[M::mem_pestat] mean and std.dev: (605.54, 466.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2945)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 70, #reads = 1082591 mem = 536878676 peak = 1091612672
[M::process] read 689176 sequences (100000161 bp) in 2.777 CPU sec, 3.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286964, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (311.70, 143.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688070 sequences (100000136 bp) in 1.907 CPU sec, 1.911 real sec...
[M::mem_process_seqs] Processed 680384 reads in 179.161 CPU sec, 5.731 real sec
[M::mem_process_seqs] Processed 683560 reads in 122.024 CPU sec, 3.877 real sec
sorting block 71, #reads = 1082338 mem = 536881501 peak = 1091612672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289082, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 269, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (290.97, 140.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687906 sequences (100000261 bp) in 2.944 CPU sec, 3.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288920, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 275, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (296.79, 143.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686928 sequences (100000215 bp) in 1.850 CPU sec, 1.852 real sec...
[M::mem_process_seqs] Processed 689176 reads in 186.199 CPU sec, 6.005 real sec
[M::mem_process_seqs] Processed 688070 reads in 120.486 CPU sec, 3.892 real sec
sorting block 72, #reads = 1081534 mem = 536876300 peak = 1091612672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288510, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (297.96, 143.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685556 sequences (100000065 bp) in 2.694 CPU sec, 3.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288367, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (302.51, 144.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685110 sequences (100000269 bp) in 1.883 CPU sec, 1.884 real sec...
[M::mem_process_seqs] Processed 687906 reads in 186.018 CPU sec, 6.012 real sec
sorting block 73, #reads = 1085025 mem = 536884287 peak = 1091612672
[M::mem_process_seqs] Processed 686928 reads in 120.002 CPU sec, 3.847 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288082, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (307.77, 146.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1084807 mem = 536879043 peak = 1131642880
[M::process] read 684820 sequences (100000099 bp) in 2.884 CPU sec, 3.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288333, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (311.31, 147.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685556 reads in 177.703 CPU sec, 5.791 real sec
[M::process] read 684012 sequences (100000230 bp) in 1.854 CPU sec, 1.901 real sec...
[M::mem_process_seqs] Processed 685110 reads in 115.314 CPU sec, 3.791 real sec
sorting block 75, #reads = 1086230 mem = 536879015 peak = 1131642880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288447, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 295, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (313.09, 148.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684066 sequences (100000072 bp) in 2.689 CPU sec, 3.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287862, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (317.02, 149.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684820 reads in 176.711 CPU sec, 5.718 real sec
[M::process] read 683686 sequences (100000256 bp) in 1.939 CPU sec, 2.058 real sec...
[M::mem_process_seqs] Processed 684012 reads in 115.638 CPU sec, 3.703 real sec
sorting block 76, #reads = 1085248 mem = 536879734 peak = 1131642880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287587, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 300, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (317.23, 149.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682946 sequences (100000272 bp) in 2.485 CPU sec, 2.787 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287988, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 301, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (317.63, 148.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682922 sequences (100000054 bp) in 1.722 CPU sec, 1.730 real sec...
[M::mem_process_seqs] Processed 684066 reads in 180.562 CPU sec, 5.839 real sec
[M::mem_process_seqs] Processed 683686 reads in 116.766 CPU sec, 3.811 real sec
sorting block 77, #reads = 1084731 mem = 536879530 peak = 1131642880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287560, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 307, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (323.74, 152.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683070 sequences (100000264 bp) in 2.785 CPU sec, 3.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287760, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 305, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (322.54, 151.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682098 sequences (100000023 bp) in 1.863 CPU sec, 1.874 real sec...
[M::mem_process_seqs] Processed 682946 reads in 177.893 CPU sec, 5.709 real sec
sorting block 78, #reads = 1083903 mem = 536882471 peak = 1131642880
[M::mem_process_seqs] Processed 682922 reads in 116.051 CPU sec, 3.709 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 288078, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 304, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (321.70, 150.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 79, #reads = 1083195 mem = 536878069 peak = 1133162496
[M::process] read 681928 sequences (100000210 bp) in 2.695 CPU sec, 3.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286854, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (320.55, 147.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681930 sequences (100000290 bp) in 1.910 CPU sec, 1.921 real sec...
[M::mem_process_seqs] Processed 683070 reads in 182.342 CPU sec, 5.838 real sec
[M::mem_process_seqs] Processed 682098 reads in 120.993 CPU sec, 3.844 real sec
sorting block 80, #reads = 1083230 mem = 536880538 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287363, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (321.86, 148.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681836 sequences (100000077 bp) in 2.692 CPU sec, 2.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287392, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (321.94, 148.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681912 sequences (100000267 bp) in 1.912 CPU sec, 1.941 real sec...
[M::mem_process_seqs] Processed 681928 reads in 184.141 CPU sec, 5.905 real sec
[M::mem_process_seqs] Processed 681930 reads in 118.560 CPU sec, 3.789 real sec
sorting block 81, #reads = 1081868 mem = 536878543 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286438, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (321.41, 148.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (486, 2311, 2311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5961)
[M::mem_pestat] mean and std.dev: (1274.91, 949.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7786)
[M::mem_pestat] skip orientation RR
[M::process] read 681896 sequences (100000180 bp) in 2.495 CPU sec, 2.629 real sec...
[M::process] read 682024 sequences (100000141 bp) in 1.780 CPU sec, 1.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286819, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (321.33, 148.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681836 reads in 180.424 CPU sec, 5.744 real sec
sorting block 82, #reads = 1081258 mem = 536879915 peak = 1133162496
[M::mem_process_seqs] Processed 681912 reads in 115.998 CPU sec, 3.683 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286983, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (320.54, 147.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682426 sequences (100000281 bp) in 2.724 CPU sec, 2.825 real sec...
[M::process] read 682018 sequences (100000137 bp) in 1.577 CPU sec, 1.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286433, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (319.34, 147.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681896 reads in 181.123 CPU sec, 5.769 real sec
sorting block 83, #reads = 1081763 mem = 536883627 peak = 1133162496
[M::mem_process_seqs] Processed 682024 reads in 117.148 CPU sec, 3.726 real sec
sorting block 84, #reads = 1080732 mem = 536879646 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287590, 6, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 305, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (242.11, 80.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 300, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (316.82, 146.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681772 sequences (100000023 bp) in 2.845 CPU sec, 2.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286964, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (319.37, 147.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681706 sequences (100000220 bp) in 1.891 CPU sec, 1.899 real sec...
[M::mem_process_seqs] Processed 682426 reads in 181.180 CPU sec, 5.757 real sec
[M::mem_process_seqs] Processed 682018 reads in 118.169 CPU sec, 3.735 real sec
sorting block 85, #reads = 1080852 mem = 536881152 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286809, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (321.11, 148.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681726 sequences (100000173 bp) in 2.884 CPU sec, 2.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286909, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 231, 1539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4155)
[M::mem_pestat] mean and std.dev: (700.11, 811.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (320.24, 147.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681824 sequences (100000070 bp) in 1.964 CPU sec, 1.972 real sec...
[M::mem_process_seqs] Processed 681772 reads in 182.492 CPU sec, 5.792 real sec
[M::mem_process_seqs] Processed 681706 reads in 119.057 CPU sec, 3.766 real sec
sorting block 86, #reads = 1081776 mem = 536877834 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286904, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (320.54, 147.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687684 sequences (100000046 bp) in 3.001 CPU sec, 3.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286855, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (321.36, 148.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690228 sequences (100000008 bp) in 1.998 CPU sec, 2.000 real sec...
[M::mem_process_seqs] Processed 681726 reads in 189.376 CPU sec, 6.025 real sec
sorting block 87, #reads = 1081356 mem = 536877736 peak = 1133162496
[M::mem_process_seqs] Processed 681824 reads in 123.273 CPU sec, 3.908 real sec
sorting block 88, #reads = 1080476 mem = 536879403 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288643, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (295.72, 140.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688796 sequences (100000266 bp) in 2.937 CPU sec, 3.074 real sec...
[M::process] read 689010 sequences (100000259 bp) in 2.060 CPU sec, 2.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289846, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 265, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (284.84, 135.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687684 reads in 209.019 CPU sec, 6.655 real sec
[M::mem_process_seqs] Processed 690228 reads in 134.762 CPU sec, 4.277 real sec
sorting block 89, #reads = 1079613 mem = 536878739 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289533, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 272, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (291.76, 138.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (832, 3272, 3272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8152)
[M::mem_pestat] mean and std.dev: (2245.30, 1265.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10592)
[M::mem_pestat] skip orientation RR
[M::process] read 687928 sequences (100000144 bp) in 2.823 CPU sec, 2.939 real sec...
[M::process] read 687190 sequences (100000092 bp) in 1.881 CPU sec, 1.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289264, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 271, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (289.59, 136.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688796 reads in 206.834 CPU sec, 6.590 real sec
[M::mem_process_seqs] Processed 689010 reads in 135.329 CPU sec, 4.310 real sec
sorting block 90, #reads = 1080855 mem = 536878546 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289118, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 275, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (293.29, 137.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (429, 429, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (191, 786)
[M::mem_pestat] mean and std.dev: (405.45, 107.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation RR
[M::process] read 686812 sequences (100000104 bp) in 2.961 CPU sec, 3.061 real sec...
[M::process] read 686026 sequences (100000242 bp) in 1.956 CPU sec, 1.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288816, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (297.44, 139.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687928 reads in 200.959 CPU sec, 6.387 real sec
sorting block 91, #reads = 1079233 mem = 536879103 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289039, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 283, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (301.04, 141.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685208 sequences (100000182 bp) in 2.489 CPU sec, 2.494 real sec...
[M::mem_process_seqs] Processed 686812 reads in 109.717 CPU sec, 3.467 real sec
[M::mem_process_seqs] Processed 687190 reads in 270.014 CPU sec, 8.583 real sec
sorting block 92, #reads = 1079567 mem = 536879020 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288649, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.96, 142.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684836 sequences (100000153 bp) in 2.936 CPU sec, 3.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288409, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (306.20, 142.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686026 reads in 208.013 CPU sec, 6.608 real sec
[M::process] read 684636 sequences (100000018 bp) in 1.898 CPU sec, 1.899 real sec...
[M::mem_process_seqs] Processed 685208 reads in 121.950 CPU sec, 3.850 real sec
sorting block 93, #reads = 1079873 mem = 536878424 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288399, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (308.06, 143.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684308 sequences (100000246 bp) in 2.908 CPU sec, 3.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287988, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (309.51, 143.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684018 sequences (100000116 bp) in 1.946 CPU sec, 1.953 real sec...
[M::mem_process_seqs] Processed 684836 reads in 183.180 CPU sec, 5.812 real sec
sorting block 94, #reads = 1081923 mem = 536878293 peak = 1133162496
[M::mem_process_seqs] Processed 684636 reads in 118.487 CPU sec, 3.749 real sec
sorting block 95, #reads = 1082670 mem = 536880520 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288084, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (312.64, 146.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683678 sequences (100000068 bp) in 2.737 CPU sec, 2.847 real sec...
[M::process] read 682986 sequences (100000203 bp) in 1.850 CPU sec, 1.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287911, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 296, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (314.24, 147.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (379, 2545, 2545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6877)
[M::mem_pestat] mean and std.dev: (1758.70, 1179.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9043)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 684308 reads in 183.662 CPU sec, 5.832 real sec
[M::mem_process_seqs] Processed 684018 reads in 118.022 CPU sec, 3.747 real sec
sorting block 96, #reads = 1081863 mem = 536877342 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287526, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 297, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (313.92, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682994 sequences (100000003 bp) in 2.827 CPU sec, 2.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287554, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 300, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (315.82, 145.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682198 sequences (100000267 bp) in 1.972 CPU sec, 1.976 real sec...
[M::mem_process_seqs] Processed 683678 reads in 178.111 CPU sec, 5.642 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 287609, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (686, 686, 916)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (226, 1376)
[M::mem_pestat] mean and std.dev: (703.43, 134.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1606)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (317.65, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682052 sequences (100000093 bp) in 2.427 CPU sec, 2.428 real sec...
[M::mem_process_seqs] Processed 682994 reads in 99.398 CPU sec, 3.139 real sec
sorting block 97, #reads = 1082982 mem = 536879230 peak = 1133162496
[M::mem_process_seqs] Processed 682986 reads in 244.029 CPU sec, 7.731 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287226, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (320.56, 148.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 98, #reads = 1081829 mem = 536879254 peak = 1133162496
[M::process] read 682000 sequences (100000172 bp) in 2.830 CPU sec, 2.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286972, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (320.62, 148.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682198 reads in 195.682 CPU sec, 6.206 real sec
[M::process] read 682194 sequences (100000099 bp) in 1.963 CPU sec, 1.984 real sec...
[M::mem_process_seqs] Processed 682052 reads in 115.372 CPU sec, 3.635 real sec
sorting block 99, #reads = 1081855 mem = 536877950 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287384, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (322.15, 149.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682370 sequences (100000211 bp) in 2.459 CPU sec, 2.594 real sec...
[M::process] read 682106 sequences (100000300 bp) in 1.840 CPU sec, 1.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287118, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (319.93, 149.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682000 reads in 173.356 CPU sec, 5.502 real sec
[M::mem_process_seqs] Processed 682194 reads in 111.014 CPU sec, 3.516 real sec
sorting block 100, #reads = 1081162 mem = 536879103 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287173, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (320.78, 150.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682606 sequences (100000147 bp) in 2.623 CPU sec, 2.728 real sec...
[M::process] read 682016 sequences (100000047 bp) in 1.830 CPU sec, 1.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287344, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (322.43, 151.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682370 reads in 174.990 CPU sec, 5.547 real sec
sorting block 101, #reads = 1083319 mem = 536878357 peak = 1133162496
[M::mem_process_seqs] Processed 682106 reads in 112.986 CPU sec, 3.582 real sec
sorting block 102, #reads = 1082771 mem = 536880737 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287411, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (320.83, 150.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682164 sequences (100000208 bp) in 2.452 CPU sec, 2.544 real sec...
[M::process] read 686298 sequences (100000165 bp) in 1.610 CPU sec, 1.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286696, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (323.85, 152.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682606 reads in 176.642 CPU sec, 5.609 real sec
[M::mem_process_seqs] Processed 682016 reads in 111.948 CPU sec, 3.565 real sec
sorting block 103, #reads = 1080782 mem = 536876801 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286876, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (324.05, 152.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688314 sequences (100000178 bp) in 2.723 CPU sec, 2.817 real sec...
[M::process] read 688444 sequences (100000275 bp) in 1.850 CPU sec, 1.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288231, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.62, 143.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682164 reads in 192.562 CPU sec, 6.112 real sec
[M::mem_process_seqs] Processed 686298 reads in 128.500 CPU sec, 4.083 real sec
sorting block 104, #reads = 1078033 mem = 536877643 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288906, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 273, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (293.35, 140.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687344 sequences (100000195 bp) in 2.508 CPU sec, 2.594 real sec...
[M::process] read 686710 sequences (100000179 bp) in 1.900 CPU sec, 1.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 289273, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 274, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (294.54, 141.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688314 reads in 178.247 CPU sec, 5.653 real sec
[M::mem_process_seqs] Processed 688444 reads in 113.715 CPU sec, 3.593 real sec
sorting block 105, #reads = 1079035 mem = 536878410 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289238, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 278, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (297.61, 141.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686326 sequences (100000190 bp) in 2.785 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288800, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (302.14, 144.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685434 sequences (100000161 bp) in 1.850 CPU sec, 1.857 real sec...
[M::mem_process_seqs] Processed 687344 reads in 171.038 CPU sec, 5.418 real sec
sorting block 106, #reads = 1085270 mem = 536878859 peak = 1133162496
[M::mem_process_seqs] Processed 686710 reads in 106.778 CPU sec, 3.377 real sec
sorting block 107, #reads = 1086367 mem = 536887396 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288916, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (304.04, 145.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684638 sequences (100000275 bp) in 2.420 CPU sec, 2.492 real sec...
[M::process] read 684778 sequences (100000295 bp) in 1.910 CPU sec, 1.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287967, 9, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (974, 1116, 1116)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (690, 1400)
[M::mem_pestat] mean and std.dev: (1075.40, 66.64)
[M::mem_pestat] low and high boundaries for proper pairs: (548, 1542)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (306.05, 143.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (381, 384, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (179, 684)
[M::mem_pestat] mean and std.dev: (410.50, 47.02)
[M::mem_pestat] low and high boundaries for proper pairs: (78, 785)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686326 reads in 165.481 CPU sec, 5.242 real sec
[M::mem_process_seqs] Processed 685434 reads in 103.877 CPU sec, 3.290 real sec
sorting block 108, #reads = 1086817 mem = 536879725 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287954, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (309.32, 145.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684632 sequences (100000008 bp) in 2.740 CPU sec, 2.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287910, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (307.74, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684114 sequences (100000121 bp) in 1.841 CPU sec, 1.847 real sec...
[M::mem_process_seqs] Processed 684638 reads in 163.473 CPU sec, 5.174 real sec
[M::mem_process_seqs] Processed 684778 reads in 104.369 CPU sec, 3.294 real sec
sorting block 109, #reads = 1085843 mem = 536876922 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288347, 11, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (308.77, 144.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (401, 1615, 1615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4043)
[M::mem_pestat] mean and std.dev: (1157.09, 607.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5257)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 684308 sequences (100000221 bp) in 2.563 CPU sec, 2.635 real sec...
[M::process] read 683912 sequences (100000142 bp) in 1.810 CPU sec, 1.807 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287469, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (310.33, 143.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684632 reads in 165.662 CPU sec, 5.251 real sec
[M::mem_process_seqs] Processed 684114 reads in 105.215 CPU sec, 3.330 real sec
sorting block 110, #reads = 1085733 mem = 536879120 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287833, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (309.66, 143.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684000 sequences (100000115 bp) in 2.548 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287420, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (310.73, 143.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684080 sequences (100000203 bp) in 1.860 CPU sec, 1.859 real sec...
[M::mem_process_seqs] Processed 684308 reads in 166.239 CPU sec, 5.272 real sec
sorting block 111, #reads = 1085483 mem = 536878380 peak = 1133162496
[M::mem_process_seqs] Processed 683912 reads in 105.281 CPU sec, 3.319 real sec
sorting block 112, #reads = 1085059 mem = 536882301 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287601, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (311.46, 145.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684644 sequences (100000046 bp) in 2.496 CPU sec, 2.564 real sec...
[M::process] read 684474 sequences (100000282 bp) in 1.870 CPU sec, 1.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 288246, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (311.61, 145.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684000 reads in 173.300 CPU sec, 5.495 real sec
[M::mem_process_seqs] Processed 684080 reads in 110.047 CPU sec, 3.485 real sec
sorting block 113, #reads = 1083876 mem = 536879902 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287961, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (305.68, 142.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684904 sequences (100000158 bp) in 2.306 CPU sec, 2.401 real sec...
[M::process] read 683670 sequences (100000027 bp) in 1.890 CPU sec, 1.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288237, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (307.89, 143.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684644 reads in 170.741 CPU sec, 5.420 real sec
[M::mem_process_seqs] Processed 684474 reads in 108.403 CPU sec, 3.435 real sec
sorting block 114, #reads = 1083620 mem = 536883436 peak = 1133162496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288196, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 166, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1697)
[M::mem_pestat] mean and std.dev: (378.60, 287.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2215)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (307.07, 144.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683496 sequences (100000285 bp) in 2.864 CPU sec, 2.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287416, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (315.75, 149.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683012 sequences (100000102 bp) in 1.910 CPU sec, 1.914 real sec...
[M::mem_process_seqs] Processed 684904 reads in 172.891 CPU sec, 5.474 real sec
sorting block 115, #reads = 1083596 mem = 536878918 peak = 1133162496
[M::mem_process_seqs] Processed 683670 reads in 109.626 CPU sec, 3.463 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287566, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (316.82, 149.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682742 sequences (100000069 bp) in 2.602 CPU sec, 2.685 real sec...
[M::process] read 682304 sequences (100000298 bp) in 1.420 CPU sec, 1.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287180, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 300, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (318.29, 149.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683496 reads in 171.465 CPU sec, 5.449 real sec
sorting block 116, #reads = 1083517 mem = 536878833 peak = 1133162496
[M::mem_process_seqs] Processed 683012 reads in 109.776 CPU sec, 3.483 real sec
sorting block 117, #reads = 1082972 mem = 536879937 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287281, 3, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 174, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437)
[M::mem_pestat] mean and std.dev: (173.25, 44.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 525)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (318.76, 148.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682364 sequences (100000027 bp) in 2.414 CPU sec, 2.476 real sec...
[M::process] read 683628 sequences (100000128 bp) in 1.820 CPU sec, 1.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287313, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (321.39, 150.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682742 reads in 168.280 CPU sec, 5.341 real sec
[M::mem_process_seqs] Processed 682304 reads in 107.762 CPU sec, 3.401 real sec
sorting block 118, #reads = 1083539 mem = 536878488 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287231, 14, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (321.35, 150.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 17, 17)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 17)
[M::mem_pestat] mean and std.dev: (17.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (17, 17)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 682850 sequences (100000211 bp) in 2.500 CPU sec, 2.572 real sec...
[M::process] read 686814 sequences (100000123 bp) in 1.880 CPU sec, 1.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287486, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (314.37, 149.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682364 reads in 171.699 CPU sec, 5.439 real sec
[M::mem_process_seqs] Processed 683628 reads in 111.511 CPU sec, 3.533 real sec
sorting block 119, #reads = 1082140 mem = 536878339 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287416, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (319.56, 151.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690370 sequences (100000132 bp) in 2.769 CPU sec, 2.816 real sec...
[M::process] read 690246 sequences (100000131 bp) in 1.890 CPU sec, 1.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288335, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (302.22, 144.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682850 reads in 188.017 CPU sec, 5.960 real sec
sorting block 120, #reads = 1080866 mem = 536878745 peak = 1156923392
[M::mem_process_seqs] Processed 686814 reads in 126.302 CPU sec, 4.012 real sec
sorting block 121, #reads = 1074577 mem = 536882819 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289574, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 266, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (285.88, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690676 sequences (100000231 bp) in 2.951 CPU sec, 3.043 real sec...
[M::process] read 689954 sequences (100000167 bp) in 1.940 CPU sec, 1.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289535, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 266, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (286.34, 136.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690370 reads in 182.488 CPU sec, 5.774 real sec
[M::mem_process_seqs] Processed 690246 reads in 116.815 CPU sec, 3.696 real sec
sorting block 122, #reads = 1082458 mem = 536876969 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289378, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 263, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (283.86, 135.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689650 sequences (100000173 bp) in 2.850 CPU sec, 2.887 real sec...
[M::process] read 688262 sequences (100000116 bp) in 1.950 CPU sec, 1.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289739, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 267, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (286.35, 135.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690676 reads in 189.155 CPU sec, 5.995 real sec
[M::mem_process_seqs] Processed 689954 reads in 118.560 CPU sec, 3.755 real sec
sorting block 123, #reads = 1079199 mem = 536879748 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289677, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 270, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (288.33, 135.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687078 sequences (100000147 bp) in 2.740 CPU sec, 2.826 real sec...
[M::process] read 686080 sequences (100000068 bp) in 1.830 CPU sec, 1.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289531, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (320, 366, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (228, 458)
[M::mem_pestat] mean and std.dev: (362.67, 15.43)
[M::mem_pestat] low and high boundaries for proper pairs: (182, 504)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 275, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (293.05, 137.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 689650 reads in 189.475 CPU sec, 6.010 real sec
sorting block 124, #reads = 1080803 mem = 536880008 peak = 1156923392
[M::mem_process_seqs] Processed 688262 reads in 119.541 CPU sec, 3.799 real sec
[M::process] read 685692 sequences (100000106 bp) in 2.435 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289085, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (298.00, 139.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685716 sequences (100000271 bp) in 1.470 CPU sec, 1.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 288238, 8, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 376, 709)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1697)
[M::mem_pestat] mean and std.dev: (415.91, 240.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2191)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.96, 141.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 687078 reads in 175.844 CPU sec, 5.592 real sec
sorting block 125, #reads = 1079138 mem = 536878394 peak = 1156923392
[M::mem_process_seqs] Processed 686080 reads in 110.526 CPU sec, 3.526 real sec
sorting block 126, #reads = 1081623 mem = 536877828 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288731, 7, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (305.63, 143.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 623, 1383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3809)
[M::mem_pestat] mean and std.dev: (934.06, 894.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5022)
[M::mem_pestat] skip orientation RR
[M::process] read 685322 sequences (100000131 bp) in 2.724 CPU sec, 2.775 real sec...
[M::process] read 685020 sequences (100000226 bp) in 1.820 CPU sec, 1.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288335, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (306.03, 144.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685692 reads in 176.372 CPU sec, 5.593 real sec
[M::mem_process_seqs] Processed 685716 reads in 111.151 CPU sec, 3.516 real sec
sorting block 127, #reads = 1082120 mem = 536878557 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288127, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (307.19, 144.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685168 sequences (100000098 bp) in 2.554 CPU sec, 2.635 real sec...
[M::process] read 684442 sequences (100000084 bp) in 1.710 CPU sec, 1.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287705, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (309.54, 145.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685322 reads in 175.265 CPU sec, 5.565 real sec
[M::mem_process_seqs] Processed 685020 reads in 110.150 CPU sec, 3.497 real sec
sorting block 128, #reads = 1082146 mem = 536879683 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288112, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (311.91, 147.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684092 sequences (100000124 bp) in 2.418 CPU sec, 2.500 real sec...
[M::process] read 683496 sequences (100000002 bp) in 1.450 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288566, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 295, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (312.79, 147.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685168 reads in 171.289 CPU sec, 5.447 real sec
sorting block 129, #reads = 1082851 mem = 536880234 peak = 1156923392
[M::mem_process_seqs] Processed 684442 reads in 116.029 CPU sec, 3.705 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287838, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (314.28, 147.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 130, #reads = 1082297 mem = 536878051 peak = 1156923392
[M::process] read 683470 sequences (100000268 bp) in 2.569 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287441, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (314.38, 145.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683064 sequences (100000229 bp) in 1.890 CPU sec, 1.886 real sec...
[M::mem_process_seqs] Processed 684092 reads in 166.550 CPU sec, 5.278 real sec
[M::mem_process_seqs] Processed 683496 reads in 105.542 CPU sec, 3.333 real sec
sorting block 131, #reads = 1084047 mem = 536878752 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287539, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (315.51, 146.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682450 sequences (100000256 bp) in 2.790 CPU sec, 2.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287580, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 302, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (318.67, 148.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681952 sequences (100000289 bp) in 1.860 CPU sec, 1.860 real sec...
[M::mem_process_seqs] Processed 683470 reads in 165.783 CPU sec, 5.250 real sec
[M::mem_process_seqs] Processed 683064 reads in 104.951 CPU sec, 3.309 real sec
sorting block 132, #reads = 1082304 mem = 536878368 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287385, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 306, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (321.49, 148.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682120 sequences (100000195 bp) in 2.766 CPU sec, 2.829 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287659, 14, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (324.18, 149.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 798, 798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2360)
[M::mem_pestat] mean and std.dev: (491.50, 386.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3141)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 682660 sequences (100000038 bp) in 1.850 CPU sec, 1.847 real sec...
[M::mem_process_seqs] Processed 682450 reads in 165.157 CPU sec, 5.229 real sec
[M::mem_process_seqs] Processed 681952 reads in 104.043 CPU sec, 3.282 real sec
sorting block 133, #reads = 1083598 mem = 536882717 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287159, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 306, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (321.61, 148.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (434, 434, 3943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10961)
[M::mem_pestat] mean and std.dev: (1230.92, 1493.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14470)
[M::mem_pestat] skip orientation RR
[M::process] read 681784 sequences (100000184 bp) in 2.505 CPU sec, 2.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287629, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (318.71, 147.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681732 sequences (100000187 bp) in 1.870 CPU sec, 1.871 real sec...
[M::mem_process_seqs] Processed 682120 reads in 167.538 CPU sec, 5.315 real sec
sorting block 134, #reads = 1083132 mem = 536880918 peak = 1156923392
[M::mem_process_seqs] Processed 682660 reads in 107.950 CPU sec, 3.407 real sec
[M::process] read 202180 sequences (29616864 bp) in 0.844 CPU sec, 0.855 real sec...
sorting block 135, #reads = 1082576 mem = 536881052 peak = 1156923392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287581, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (321.21, 147.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 268, 7864)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23176)
[M::mem_pestat] mean and std.dev: (2301.64, 3406.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 30832)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 287035, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 378, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (318.43, 140.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 306, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (321.15, 146.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 85305, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (313.35, 142.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681784 reads in 181.587 CPU sec, 5.863 real sec
[M::mem_process_seqs] Processed 681732 reads in 124.244 CPU sec, 4.012 real sec
[M::mem_process_seqs] Processed 202180 reads in 129.788 CPU sec, 4.202 real sec
sorting block 136, #reads = 1083421 mem = 536878182 peak = 1156923392
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 148494690
overall: 69825249280 mem 20003.024 user 438.323 sys 672.069 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cAd-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:659_cAd-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cAd-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cAd-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cAd-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 672.108 sec; CPU: 20441.350 sec
sorting block 137, #reads = 569208 mem = 282771124 peak = 1156923392
uniq header count 1
merging 138 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8075545441
output reads: 149070643
bam_mem_sort: 138 calls 75.132 user 0.982 sys 76.442 real
bam_write: 138 calls 45.439 user 2.657 sys 60.653 real
execute: 1 calls 43.583 user 1.521 sys 727.445 real
merge_files: 1 calls 43.559 user 1.496 sys 54.943 real
parse_chunk: 2201 calls 93.517 user 22.203 sys 121.414 real
read_chunk: 5933 calls 47.869 user 42.455 sys 624.155 real
sort_block: 1 calls 33.223 user 41.709 sys 601.450 real
write_chunk: 3730 calls 43.111 user 1.213 sys 53.931 real
overall: 3275571200 mem 2128.121 user 83.501 sys 727.474 real
ls: cannot access '*.cram': No such file or directory