Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ad/e9eeae52324602c205351dbf3199a5/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f0/fd411bfa2c85ed253ff7dbce25bc8c/1029_N5D-T1-TDNA-1_A23MHWWLT4_1/0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f0/fd411bfa2c85ed253ff7dbce25bc8c/1029_N5D-T1-TDNA-1_A23MHWWLT4_1/0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ad/e9eeae52324602c205351dbf3199a5/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1730.916 CPU sec, 65.135 real sec
[M::process] read 681130 sequences (100000120 bp) in 0.962 CPU sec, 0.969 real sec...
[M::process] read 681564 sequences (100000192 bp) in 1.160 CPU sec, 1.190 real sec...
[M::process] read 682626 sequences (100000231 bp) in 1.221 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277871, 9, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (312.93, 133.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 353, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (366.85, 174.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 337, 678)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1522)
[M::mem_pestat] mean and std.dev: (378.96, 229.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1944)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680176 sequences (100000133 bp) in 1.166 CPU sec, 1.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 277862, 4, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 261, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (255.36, 107.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 352, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (367.05, 175.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 341, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101)
[M::mem_pestat] mean and std.dev: (391.14, 227.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1398)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 278651, 3, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (321.89, 177.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (358.44, 173.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 344, 1021)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2713)
[M::mem_pestat] mean and std.dev: (386.76, 276.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3559)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681130 reads in 247.176 CPU sec, 7.961 real sec
[M::mem_process_seqs] Processed 681564 reads in 162.629 CPU sec, 5.285 real sec
[M::mem_process_seqs] Processed 682626 reads in 177.819 CPU sec, 5.780 real sec
sorting block 0, #reads = 1068483 mem = 536885517 peak = 599617536
[M::process] read 681818 sequences (100000149 bp) in 1.683 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277373, 6, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 403, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (280.22, 112.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 363, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (375.40, 177.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 304, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (355.42, 202.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681282 sequences (100000128 bp) in 1.238 CPU sec, 1.321 real sec...
[M::process] read 680670 sequences (100000177 bp) in 1.100 CPU sec, 1.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277892, 13, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 219, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (237.56, 108.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 348, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (364.21, 175.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (920, 1438, 3223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7829)
[M::mem_pestat] mean and std.dev: (1497.85, 1093.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10132)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 220, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (258.24, 123.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680176 reads in 148.741 CPU sec, 4.727 real sec
sorting block 1, #reads = 1070693 mem = 536879756 peak = 870150144
[M::mem_process_seqs] Processed 681818 reads in 96.395 CPU sec, 3.060 real sec
sorting block 2, #reads = 1070596 mem = 536883924 peak = 974884864
[M::process] read 680538 sequences (100000145 bp) in 1.542 CPU sec, 1.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278173, 2, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 197, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219)
[M::mem_pestat] mean and std.dev: (265.88, 199.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 347, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (362.99, 173.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 319, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (299.85, 103.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681280 sequences (100000074 bp) in 1.190 CPU sec, 1.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277036, 10, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 201, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (256.07, 146.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 350, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (364.88, 172.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (652, 2225, 2225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5371)
[M::mem_pestat] mean and std.dev: (1593.00, 835.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6944)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 318, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (322.86, 182.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1385)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 277210, 7, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 184, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (203.80, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 350, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (364.85, 172.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 320, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (344.35, 171.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1323)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681282 reads in 214.529 CPU sec, 6.862 real sec
[M::mem_process_seqs] Processed 680670 reads in 161.050 CPU sec, 5.182 real sec
[M::mem_process_seqs] Processed 680538 reads in 176.351 CPU sec, 5.692 real sec
sorting block 3, #reads = 1069328 mem = 536881490 peak = 974884864
[M::process] read 679872 sequences (100000155 bp) in 1.796 CPU sec, 1.921 real sec...
sorting block 4, #reads = 1069970 mem = 536877198 peak = 974884864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278039, 3, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 284, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (284.26, 150.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1337)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (359.58, 171.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 275, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (284.67, 124.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680932 sequences (100000074 bp) in 1.416 CPU sec, 1.473 real sec...
[M::process] read 680702 sequences (100000093 bp) in 1.160 CPU sec, 1.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 276873, 5, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 189, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (172.19, 43.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 352, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (365.79, 171.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 475, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191)
[M::mem_pestat] mean and std.dev: (421.58, 224.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1505)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681280 reads in 151.924 CPU sec, 4.832 real sec
[M::mem_process_seqs] Processed 679872 reads in 97.257 CPU sec, 3.086 real sec
sorting block 5, #reads = 1070142 mem = 536880166 peak = 974884864
[M::process] read 680224 sequences (100000163 bp) in 1.675 CPU sec, 1.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278034, 4, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 252, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (246.60, 130.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (360.19, 171.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 371, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069)
[M::mem_pestat] mean and std.dev: (331.47, 187.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1364)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681354 sequences (100000134 bp) in 1.180 CPU sec, 1.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 277448, 9, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 235, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (244.96, 143.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (363.30, 173.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 300, 776)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908)
[M::mem_pestat] mean and std.dev: (448.90, 357.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2474)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 277988, 3, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 269, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (273.00, 136.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 355, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (371.01, 177.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 286, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (299.47, 132.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680932 reads in 210.079 CPU sec, 6.717 real sec
[M::mem_process_seqs] Processed 680702 reads in 157.422 CPU sec, 5.052 real sec
sorting block 6, #reads = 1070391 mem = 536877234 peak = 974884864
[M::mem_process_seqs] Processed 680224 reads in 172.362 CPU sec, 5.561 real sec
[M::process] read 681520 sequences (100000102 bp) in 1.787 CPU sec, 1.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278568, 8, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 306, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (323.55, 184.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1400)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 349, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (364.09, 174.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 238, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (278.70, 190.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 7, #reads = 1069499 mem = 536881679 peak = 974884864
[M::process] read 681730 sequences (100000003 bp) in 1.530 CPU sec, 1.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278471, 11, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 332, 808)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1996)
[M::mem_pestat] mean and std.dev: (406.36, 299.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2590)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (367.11, 176.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (911, 1829, 1829)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3665)
[M::mem_pestat] mean and std.dev: (1398.73, 599.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4583)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 328, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (311.55, 150.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681354 reads in 143.210 CPU sec, 4.561 real sec
[M::process] read 680364 sequences (100000273 bp) in 1.308 CPU sec, 1.325 real sec...
[M::mem_process_seqs] Processed 681520 reads in 93.555 CPU sec, 2.956 real sec
sorting block 8, #reads = 1071511 mem = 536879741 peak = 974884864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 278015, 6, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 260, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147)
[M::mem_pestat] mean and std.dev: (321.52, 221.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1480)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 351, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (364.91, 175.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 317, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (303.95, 119.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681334 sequences (100000021 bp) in 1.867 CPU sec, 2.027 real sec...
[M::process] read 680682 sequences (100000248 bp) in 1.210 CPU sec, 1.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277803, 5, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 339, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188)
[M::mem_pestat] mean and std.dev: (381.60, 261.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 357, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (370.77, 176.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 282, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (329.53, 191.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1328)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681730 reads in 141.760 CPU sec, 4.523 real sec
[M::mem_process_seqs] Processed 680364 reads in 91.271 CPU sec, 2.912 real sec
sorting block 9, #reads = 1072213 mem = 536884883 peak = 974884864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278278, 5, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 383, 561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197)
[M::mem_pestat] mean and std.dev: (368.57, 189.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 350, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (365.56, 175.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 331, 618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448)
[M::mem_pestat] mean and std.dev: (481.78, 415.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2145)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 679806 sequences (100000274 bp) in 1.891 CPU sec, 2.014 real sec...
[M::process] read 680950 sequences (100000010 bp) in 1.230 CPU sec, 1.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 278054, 5, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 294, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (254.43, 105.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 351, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (366.90, 175.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 283, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (274.47, 104.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681334 reads in 143.122 CPU sec, 4.554 real sec
sorting block 10, #reads = 1072500 mem = 536878101 peak = 974884864
[M::mem_process_seqs] Processed 680682 reads in 92.976 CPU sec, 2.959 real sec
sorting block 11, #reads = 1070531 mem = 536879598 peak = 985047040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277935, 3, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (247, 360, 638)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420)
[M::mem_pestat] mean and std.dev: (429.88, 295.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1811)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 356, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (371.57, 175.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344)
[M::mem_pestat] mean and std.dev: (394.24, 228.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1717)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681440 sequences (100000003 bp) in 1.949 CPU sec, 2.084 real sec...
[M::process] read 680070 sequences (100000038 bp) in 1.230 CPU sec, 1.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278045, 1, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 286, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262)
[M::mem_pestat] mean and std.dev: (351.97, 224.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1619)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (364.80, 175.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 320, 709)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1701)
[M::mem_pestat] mean and std.dev: (451.50, 358.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2197)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 679806 reads in 144.510 CPU sec, 4.597 real sec
[M::mem_process_seqs] Processed 680950 reads in 94.205 CPU sec, 3.001 real sec
sorting block 12, #reads = 1071156 mem = 536881560 peak = 985047040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278394, 5, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 231, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (242.59, 120.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (360.96, 174.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 312, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199)
[M::mem_pestat] mean and std.dev: (316.15, 185.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1542)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681104 sequences (100000035 bp) in 2.081 CPU sec, 2.189 real sec...
[M::process] read 681222 sequences (100000167 bp) in 1.290 CPU sec, 1.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 276831, 5, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 242, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (265.87, 114.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 351, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (367.56, 174.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 291, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (305.38, 141.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681440 reads in 144.270 CPU sec, 4.587 real sec
[M::mem_process_seqs] Processed 680070 reads in 93.624 CPU sec, 2.980 real sec
sorting block 13, #reads = 1071558 mem = 536878452 peak = 985047040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 277817, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 224, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282)
[M::mem_pestat] mean and std.dev: (298.09, 161.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1656)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (360.89, 173.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (265.36, 120.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681910 sequences (100000185 bp) in 2.089 CPU sec, 2.187 real sec...
[M::process] read 682786 sequences (100000243 bp) in 1.300 CPU sec, 1.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278216, 4, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 293, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317)
[M::mem_pestat] mean and std.dev: (380.04, 272.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1696)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (362.93, 175.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 289, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321)
[M::mem_pestat] mean and std.dev: (313.00, 232.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1702)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681104 reads in 144.312 CPU sec, 4.589 real sec
sorting block 14, #reads = 1069606 mem = 536880441 peak = 985047040
[M::mem_process_seqs] Processed 681222 reads in 93.464 CPU sec, 2.971 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278935, 7, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (253.14, 75.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 633)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (360.48, 174.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 307, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (301.80, 132.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681694 sequences (100000058 bp) in 2.130 CPU sec, 2.229 real sec...
[M::process] read 680162 sequences (100000205 bp) in 1.310 CPU sec, 1.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278492, 3, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 237, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (238.59, 91.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (353.33, 167.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 254, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (249.08, 100.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681910 reads in 148.206 CPU sec, 4.715 real sec
sorting block 15, #reads = 1069673 mem = 536880784 peak = 985047040
[M::mem_process_seqs] Processed 682786 reads in 96.437 CPU sec, 3.067 real sec
sorting block 16, #reads = 1068835 mem = 536879204 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278295, 2, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 269, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (263.14, 129.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (359.29, 169.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 235, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (216.32, 89.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681908 sequences (100000145 bp) in 2.237 CPU sec, 2.399 real sec...
[M::process] read 681732 sequences (100000192 bp) in 1.300 CPU sec, 1.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278536, 2, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 267, 569)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1397)
[M::mem_pestat] mean and std.dev: (267.72, 139.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1811)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 359, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (370.39, 172.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 288, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206)
[M::mem_pestat] mean and std.dev: (327.35, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1539)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681694 reads in 146.587 CPU sec, 4.661 real sec
[M::mem_process_seqs] Processed 680162 reads in 94.586 CPU sec, 3.006 real sec
sorting block 17, #reads = 1070554 mem = 536879251 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 278130, 8, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 220, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (255.11, 111.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 700)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (359.80, 171.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 445, 885)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2223)
[M::mem_pestat] mean and std.dev: (474.04, 353.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2892)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680662 sequences (100000063 bp) in 2.131 CPU sec, 2.256 real sec...
[M::process] read 680432 sequences (100000070 bp) in 1.310 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278115, 7, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 328, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (259.80, 107.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (360.18, 170.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 251, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (269.23, 107.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681908 reads in 147.553 CPU sec, 4.692 real sec
[M::mem_process_seqs] Processed 681732 reads in 95.823 CPU sec, 3.050 real sec
sorting block 18, #reads = 1070336 mem = 536879018 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277768, 3, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 220, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (238.90, 136.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 354, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (366.75, 172.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 361, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (348.45, 159.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681054 sequences (100000213 bp) in 2.033 CPU sec, 2.137 real sec...
[M::process] read 681180 sequences (100000123 bp) in 1.260 CPU sec, 1.264 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 278035, 5, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 311, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (290.05, 136.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 353, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (366.84, 172.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 300, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361)
[M::mem_pestat] mean and std.dev: (320.60, 185.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1749)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680662 reads in 149.764 CPU sec, 4.765 real sec
sorting block 19, #reads = 1069935 mem = 536878591 peak = 1062576128
[M::process] read 681366 sequences (100000140 bp) in 1.505 CPU sec, 1.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277679, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 190, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 420)
[M::mem_pestat] mean and std.dev: (191.88, 65.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 503)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 349, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (364.13, 173.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 317, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (296.26, 126.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681054 reads in 89.586 CPU sec, 3.114 real sec
sorting block 20, #reads = 1070097 mem = 536878186 peak = 1062576128
[M::mem_process_seqs] Processed 680432 reads in 205.991 CPU sec, 6.835 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 276804, 2, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 228, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (239.97, 86.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (359.40, 171.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 293, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (314.19, 175.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681274 sequences (100000033 bp) in 2.099 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277692, 10, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 281, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729)
[M::mem_pestat] mean and std.dev: (372.80, 282.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2259)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (359.00, 172.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1171, 1171, 1652)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (209, 2614)
[M::mem_pestat] mean and std.dev: (1271.60, 506.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3300)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 289, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (263.13, 99.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681254 sequences (100000233 bp) in 1.290 CPU sec, 1.286 real sec...
[M::mem_process_seqs] Processed 681180 reads in 167.703 CPU sec, 5.335 real sec
sorting block 21, #reads = 1070301 mem = 536878098 peak = 1062576128
[M::mem_process_seqs] Processed 681366 reads in 98.413 CPU sec, 3.124 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277985, 7, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 262, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.73, 154.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (359.82, 173.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 307, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (306.81, 113.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1070614 mem = 536876299 peak = 1062576128
[M::process] read 681524 sequences (100000285 bp) in 1.921 CPU sec, 2.035 real sec...
[M::process] read 680172 sequences (100000142 bp) in 1.260 CPU sec, 1.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278075, 8, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 281, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (273.17, 132.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (362.74, 175.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 303, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207)
[M::mem_pestat] mean and std.dev: (306.80, 185.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1538)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681274 reads in 146.557 CPU sec, 4.671 real sec
[M::mem_process_seqs] Processed 681254 reads in 94.824 CPU sec, 3.020 real sec
sorting block 23, #reads = 1069083 mem = 536878477 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277874, 4, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 278, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (273.55, 127.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (358.84, 171.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 294, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (300.49, 176.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681196 sequences (100000260 bp) in 1.972 CPU sec, 2.071 real sec...
[M::process] read 679938 sequences (100000104 bp) in 1.280 CPU sec, 1.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277827, 7, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 286, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (276.55, 157.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (364.83, 169.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 248, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (226.96, 91.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681524 reads in 147.145 CPU sec, 4.682 real sec
[M::mem_process_seqs] Processed 680172 reads in 94.330 CPU sec, 3.008 real sec
sorting block 24, #reads = 1069489 mem = 536878288 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 277411, 3, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 297, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (274.86, 98.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 344, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (357.70, 167.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (307.76, 159.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682180 sequences (100000271 bp) in 1.944 CPU sec, 2.108 real sec...
[M::process] read 682292 sequences (100000287 bp) in 1.300 CPU sec, 1.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 276669, 13, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 287, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133)
[M::mem_pestat] mean and std.dev: (298.40, 197.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1467)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 359, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (370.04, 171.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (360, 360, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (313.27, 130.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 241, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (313.64, 175.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681196 reads in 147.371 CPU sec, 4.701 real sec
[M::mem_process_seqs] Processed 679938 reads in 95.616 CPU sec, 3.041 real sec
sorting block 25, #reads = 1072395 mem = 536878365 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278044, 8, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 264, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (283.00, 124.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 344, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (358.09, 170.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 251, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (276.94, 124.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680954 sequences (100000026 bp) in 2.150 CPU sec, 2.272 real sec...
[M::process] read 681114 sequences (100000083 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277984, 9, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 326, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (300.43, 137.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (356.15, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 372, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214)
[M::mem_pestat] mean and std.dev: (312.73, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1557)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682180 reads in 150.696 CPU sec, 4.795 real sec
sorting block 26, #reads = 1070653 mem = 536879355 peak = 1062576128
[M::mem_process_seqs] Processed 682292 reads in 97.910 CPU sec, 3.114 real sec
sorting block 27, #reads = 1071041 mem = 536880664 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 277102, 9, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 282, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (306.41, 182.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 350, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (363.34, 171.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 348, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 662)
[M::mem_pestat] mean and std.dev: (333.23, 116.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680730 sequences (100000109 bp) in 1.891 CPU sec, 2.010 real sec...
[M::process] read 681160 sequences (100000128 bp) in 1.305 CPU sec, 1.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 277338, 4, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 322, 583)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277)
[M::mem_pestat] mean and std.dev: (399.56, 300.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1624)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 350, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (363.50, 171.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 334, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (295.18, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680954 reads in 154.175 CPU sec, 4.915 real sec
[M::mem_process_seqs] Processed 681114 reads in 98.920 CPU sec, 3.152 real sec
sorting block 28, #reads = 1070035 mem = 536880029 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277060, 7, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 251, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (266.75, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 352, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (365.83, 172.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 299, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (288.50, 99.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681574 sequences (100000146 bp) in 2.182 CPU sec, 2.271 real sec...
[M::process] read 681550 sequences (100000162 bp) in 1.310 CPU sec, 1.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 277333, 7, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 516, 618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394)
[M::mem_pestat] mean and std.dev: (392.40, 185.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (359.81, 170.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 254, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (306.90, 146.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680730 reads in 154.763 CPU sec, 4.927 real sec
[M::mem_process_seqs] Processed 681160 reads in 99.050 CPU sec, 3.157 real sec
sorting block 29, #reads = 1069565 mem = 536884276 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277595, 4, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (236.97, 111.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (355.60, 169.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 211, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (251.14, 132.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681440 sequences (100000089 bp) in 1.938 CPU sec, 2.030 real sec...
[M::process] read 683932 sequences (100000221 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277442, 3, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 258, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (56, 441)
[M::mem_pestat] mean and std.dev: (233.49, 66.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 518)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (357.29, 171.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 332, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200)
[M::mem_pestat] mean and std.dev: (381.69, 269.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1528)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681574 reads in 153.859 CPU sec, 4.902 real sec
sorting block 30, #reads = 1069951 mem = 536880214 peak = 1062576128
[M::mem_process_seqs] Processed 681550 reads in 97.950 CPU sec, 3.124 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277955, 6, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (270, 398, 1254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3222)
[M::mem_pestat] mean and std.dev: (676.38, 672.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4206)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 342, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (359.38, 172.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (278.74, 102.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 31, #reads = 1068855 mem = 536882289 peak = 1062576128
[M::process] read 683892 sequences (100000033 bp) in 2.258 CPU sec, 2.377 real sec...
[M::process] read 684218 sequences (100000089 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279133, 7, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113)
[M::mem_pestat] mean and std.dev: (334.23, 146.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1409)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 336, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (356.78, 177.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 228, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (237.28, 89.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681440 reads in 154.003 CPU sec, 4.899 real sec
[M::mem_process_seqs] Processed 683932 reads in 101.785 CPU sec, 3.242 real sec
sorting block 32, #reads = 1067817 mem = 536879505 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 279410, 7, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (260.27, 109.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 337, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (355.78, 175.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 306, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (274.93, 113.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682866 sequences (100000049 bp) in 2.010 CPU sec, 2.143 real sec...
[M::process] read 684518 sequences (100000271 bp) in 1.350 CPU sec, 1.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279999, 1, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 268, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (296.57, 167.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 333, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (352.68, 173.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 253, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357)
[M::mem_pestat] mean and std.dev: (349.06, 194.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1749)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683892 reads in 155.226 CPU sec, 4.944 real sec
[M::mem_process_seqs] Processed 684218 reads in 100.138 CPU sec, 3.189 real sec
sorting block 33, #reads = 1068321 mem = 536884818 peak = 1062576128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 279050, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 197, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (209.92, 101.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 343, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (359.58, 174.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 313, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (326.03, 118.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681774 sequences (100000230 bp) in 2.221 CPU sec, 2.329 real sec...
[M::process] read 682106 sequences (100000140 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279430, 7, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 225, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (278.97, 145.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 332, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (349.95, 171.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 285, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (286.86, 115.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682866 reads in 157.042 CPU sec, 4.996 real sec
sorting block 34, #reads = 1068240 mem = 536877183 peak = 1067937792
[M::process] read 681848 sequences (100000040 bp) in 1.509 CPU sec, 1.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278146, 15, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 199, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (233.36, 104.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 351, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (365.77, 175.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (481, 629, 2137)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5449)
[M::mem_pestat] mean and std.dev: (1214.93, 1265.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7105)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 326, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122)
[M::mem_pestat] mean and std.dev: (329.60, 156.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1430)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681774 reads in 93.972 CPU sec, 3.238 real sec
[M::mem_process_seqs] Processed 684518 reads in 216.855 CPU sec, 7.160 real sec
sorting block 35, #reads = 1066592 mem = 536880653 peak = 1067937792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278229, 9, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 254, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (274.31, 126.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 346, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (361.60, 173.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469)
[M::mem_pestat] mean and std.dev: (359.41, 217.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1890)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681022 sequences (100000094 bp) in 2.053 CPU sec, 2.167 real sec...
[M::process] read 681682 sequences (100000233 bp) in 1.320 CPU sec, 1.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278468, 4, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 228, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (220.29, 62.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 609)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (357.74, 169.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 303, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (303.16, 104.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682106 reads in 177.430 CPU sec, 5.652 real sec
[M::mem_process_seqs] Processed 681848 reads in 105.266 CPU sec, 3.344 real sec
sorting block 36, #reads = 1066475 mem = 536881843 peak = 1067937792
[M::process] read 681340 sequences (100000081 bp) in 2.147 CPU sec, 2.302 real sec...
[M::process] read 682838 sequences (100000264 bp) in 1.340 CPU sec, 1.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278845, 5, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 184, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (252.54, 169.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 343, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (359.15, 171.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 271, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (308.74, 149.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681682 reads in 86.174 CPU sec, 2.773 real sec
sorting block 37, #reads = 1064170 mem = 536878181 peak = 1067937792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278275, 3, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 230, 1386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3856)
[M::mem_pestat] mean and std.dev: (655.12, 817.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5091)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 354, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (367.17, 173.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 301, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (356.57, 202.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686736 sequences (100000263 bp) in 1.576 CPU sec, 1.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278761, 9, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 271, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (319.79, 166.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 347, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (363.64, 174.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 169, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495)
[M::mem_pestat] mean and std.dev: (188.60, 76.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681022 reads in 246.935 CPU sec, 7.885 real sec
[M::mem_process_seqs] Processed 681340 reads in 127.474 CPU sec, 4.136 real sec
sorting block 38, #reads = 1065156 mem = 536882454 peak = 1067937792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278920, 3, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 199, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (214.26, 79.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 341, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (358.97, 175.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 271, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192)
[M::mem_pestat] mean and std.dev: (340.32, 219.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1522)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685482 sequences (100000134 bp) in 2.144 CPU sec, 2.275 real sec...
[M::process] read 684232 sequences (100000038 bp) in 1.310 CPU sec, 1.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280989, 5, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 202, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (237.24, 122.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 318, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (335.75, 164.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 306, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (284.26, 129.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682838 reads in 166.955 CPU sec, 5.323 real sec
sorting block 39, #reads = 1065259 mem = 536879647 peak = 1067937792
[M::mem_process_seqs] Processed 686736 reads in 111.626 CPU sec, 3.561 real sec
sorting block 40, #reads = 1062164 mem = 536878609 peak = 1086259200
[M::process] read 685044 sequences (100000020 bp) in 1.955 CPU sec, 2.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280782, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 208, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (228.15, 95.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (340.64, 165.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 259, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (315.25, 187.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1459)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682324 sequences (100000216 bp) in 1.340 CPU sec, 1.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 280548, 6, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 351, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456)
[M::mem_pestat] mean and std.dev: (331.71, 209.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1890)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (348.39, 168.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (280.22, 169.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685482 reads in 163.246 CPU sec, 5.199 real sec
[M::mem_process_seqs] Processed 684232 reads in 103.615 CPU sec, 3.313 real sec
sorting block 41, #reads = 1063702 mem = 536879911 peak = 1086259200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281011, 7, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 345, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416)
[M::mem_pestat] mean and std.dev: (404.04, 296.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1826)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (343.51, 167.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 296, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (308.61, 158.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682600 sequences (100000121 bp) in 2.018 CPU sec, 2.107 real sec...
[M::process] read 681806 sequences (100000231 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279379, 7, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 253, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (222.59, 75.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 346, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (360.69, 173.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 287, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080)
[M::mem_pestat] mean and std.dev: (310.96, 139.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685044 reads in 159.419 CPU sec, 5.073 real sec
[M::mem_process_seqs] Processed 682324 reads in 103.340 CPU sec, 3.298 real sec
sorting block 42, #reads = 1066849 mem = 536879142 peak = 1086259200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 279264, 4, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (257, 333, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (321.84, 118.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 344, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (360.38, 174.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (324, 555, 757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623)
[M::mem_pestat] mean and std.dev: (544.63, 327.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2056)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680542 sequences (100000216 bp) in 2.150 CPU sec, 2.267 real sec...
[M::process] read 681888 sequences (100000176 bp) in 1.360 CPU sec, 1.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278673, 3, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 429, 917)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2331)
[M::mem_pestat] mean and std.dev: (591.40, 592.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3038)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 344, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (361.46, 174.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 267, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (302.76, 128.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682600 reads in 159.684 CPU sec, 5.082 real sec
sorting block 43, #reads = 1065166 mem = 536880268 peak = 1097961472
[M::mem_process_seqs] Processed 681806 reads in 104.101 CPU sec, 3.319 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 278165, 5, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 313, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (328.61, 181.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 356, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (371.52, 178.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 341, 677)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623)
[M::mem_pestat] mean and std.dev: (454.49, 365.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2096)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681974 sequences (100000120 bp) in 2.053 CPU sec, 2.157 real sec...
[M::process] read 683346 sequences (100000103 bp) in 1.380 CPU sec, 1.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278906, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 241, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (299.43, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 344, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (362.20, 176.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 271, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (278.31, 122.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680542 reads in 156.398 CPU sec, 4.973 real sec
sorting block 44, #reads = 1064790 mem = 536887449 peak = 1097961472
[M::mem_process_seqs] Processed 681888 reads in 102.247 CPU sec, 3.253 real sec
sorting block 45, #reads = 1065641 mem = 536877618 peak = 1152360448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 279151, 3, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 231, 580)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378)
[M::mem_pestat] mean and std.dev: (288.43, 191.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1777)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 350, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (368.27, 181.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 289, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (314.67, 141.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684346 sequences (100000219 bp) in 1.950 CPU sec, 2.067 real sec...
[M::process] read 683286 sequences (100000037 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279186, 2, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 249, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (310.46, 228.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1526)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 336, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (355.57, 175.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 296, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (290.62, 128.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681974 reads in 163.959 CPU sec, 5.226 real sec
[M::mem_process_seqs] Processed 683346 reads in 111.223 CPU sec, 3.543 real sec
sorting block 46, #reads = 1065450 mem = 536880415 peak = 1152360448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279443, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 244, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (272.05, 104.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 893)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 333, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (348.86, 169.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 272, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (302.74, 195.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1382)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682410 sequences (100000150 bp) in 2.271 CPU sec, 2.374 real sec...
[M::process] read 685104 sequences (100000240 bp) in 1.350 CPU sec, 1.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279360, 7, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 270, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (315.33, 205.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1473)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (355.99, 171.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 202, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (255.71, 121.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684346 reads in 153.561 CPU sec, 4.881 real sec
[M::mem_process_seqs] Processed 683286 reads in 98.554 CPU sec, 3.142 real sec
sorting block 47, #reads = 1062521 mem = 536878847 peak = 1152360448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 279612, 4, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (193.97, 60.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 345, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (357.81, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 285, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (284.17, 106.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683812 sequences (100000063 bp) in 2.074 CPU sec, 2.163 real sec...
[M::process] read 682198 sequences (100000176 bp) in 1.330 CPU sec, 1.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 280648, 6, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (274.93, 121.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 324, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (340.51, 164.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 236, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108)
[M::mem_pestat] mean and std.dev: (302.46, 164.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1414)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682410 reads in 153.662 CPU sec, 4.893 real sec
sorting block 48, #reads = 1067954 mem = 536881535 peak = 1152360448
[M::mem_process_seqs] Processed 685104 reads in 99.866 CPU sec, 3.181 real sec
sorting block 49, #reads = 1068807 mem = 536881577 peak = 1152360448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 279827, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 215, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (287.13, 248.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1403)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (348.02, 167.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 315, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (329.95, 199.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1461)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683660 sequences (100000161 bp) in 2.043 CPU sec, 2.149 real sec...
[M::process] read 683052 sequences (100000291 bp) in 1.330 CPU sec, 1.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278801, 10, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 237, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (244.71, 123.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (359.43, 170.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (572, 2052, 6030)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16946)
[M::mem_pestat] mean and std.dev: (2538.50, 2370.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22404)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116)
[M::mem_pestat] mean and std.dev: (269.72, 136.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1430)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683812 reads in 154.730 CPU sec, 4.926 real sec
[M::mem_process_seqs] Processed 682198 reads in 99.775 CPU sec, 3.172 real sec
sorting block 50, #reads = 1068055 mem = 536881245 peak = 1152360448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279050, 6, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 208, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (252.23, 127.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 336, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (350.40, 168.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 280, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (271.87, 98.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682776 sequences (100000258 bp) in 2.194 CPU sec, 2.326 real sec...
[M::process] read 682684 sequences (100000300 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278622, 1, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 194, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113)
[M::mem_pestat] mean and std.dev: (231.00, 139.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1436)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 340, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (354.94, 170.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 371, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262)
[M::mem_pestat] mean and std.dev: (353.08, 202.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1619)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683660 reads in 157.773 CPU sec, 5.019 real sec
[M::mem_process_seqs] Processed 683052 reads in 102.262 CPU sec, 3.260 real sec
sorting block 51, #reads = 1067980 mem = 536877931 peak = 1152360448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278538, 2, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (264.00, 142.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 341, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (357.52, 172.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 213, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (224.39, 99.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683408 sequences (100000164 bp) in 2.253 CPU sec, 2.338 real sec...
[M::process] read 686800 sequences (100000286 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278906, 3, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 332, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318)
[M::mem_pestat] mean and std.dev: (369.46, 263.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1700)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 342, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (359.55, 174.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 251, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107)
[M::mem_pestat] mean and std.dev: (313.36, 177.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1421)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682776 reads in 161.888 CPU sec, 5.150 real sec
sorting block 52, #reads = 1066496 mem = 536885538 peak = 1152360448
[M::mem_process_seqs] Processed 682684 reads in 105.001 CPU sec, 3.342 real sec
[M::process] read 686966 sequences (100000008 bp) in 2.108 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 279229, 4, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 262, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (259.33, 136.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (353.17, 174.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 241, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (237.48, 98.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685126 sequences (100000001 bp) in 1.370 CPU sec, 1.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279956, 4, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 199, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (220.82, 92.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (337.03, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 273, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (258.89, 104.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683408 reads in 173.088 CPU sec, 5.511 real sec
sorting block 53, #reads = 1065362 mem = 536876021 peak = 1152360448
[M::mem_process_seqs] Processed 686800 reads in 117.541 CPU sec, 3.759 real sec
sorting block 54, #reads = 1059185 mem = 536881095 peak = 1152360448
[M::process] read 687448 sequences (100000182 bp) in 2.303 CPU sec, 2.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 281024, 8, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 432, 1208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3224)
[M::mem_pestat] mean and std.dev: (442.65, 409.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4232)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (331.57, 159.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (288.89, 103.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685474 sequences (100000031 bp) in 1.370 CPU sec, 1.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281117, 5, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 203, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (190.81, 64.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 551)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 311, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (328.40, 159.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 285, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (274.81, 115.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280827, 15, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 182, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (242.44, 139.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 330, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (343.86, 164.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (719, 1424, 2369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5669)
[M::mem_pestat] mean and std.dev: (1337.20, 701.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7319)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 321, 873)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153)
[M::mem_pestat] mean and std.dev: (439.51, 288.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2793)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686966 reads in 241.551 CPU sec, 7.718 real sec
[M::mem_process_seqs] Processed 687448 reads in 180.050 CPU sec, 5.775 real sec
[M::mem_process_seqs] Processed 685126 reads in 197.558 CPU sec, 6.357 real sec
sorting block 55, #reads = 1061947 mem = 536880719 peak = 1152360448
[M::process] read 683752 sequences (100000280 bp) in 2.584 CPU sec, 2.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279696, 4, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 391, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313)
[M::mem_pestat] mean and std.dev: (383.73, 202.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1686)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 321, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (336.02, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 253, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (249.04, 111.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685474 reads in 92.373 CPU sec, 3.046 real sec
sorting block 56, #reads = 1063833 mem = 536882239 peak = 1152360448
[M::process] read 684014 sequences (100000079 bp) in 2.023 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 279199, 4, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 232, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (252.84, 134.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (344.63, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 257, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (274.19, 158.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683690 sequences (100000011 bp) in 1.535 CPU sec, 1.593 real sec...
[M::process] read 683226 sequences (100000007 bp) in 1.280 CPU sec, 1.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279330, 2, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 246, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (273.58, 151.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (344.80, 163.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 254, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (284.58, 136.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683752 reads in 184.347 CPU sec, 5.907 real sec
sorting block 57, #reads = 1065558 mem = 536880090 peak = 1152360448
[M::mem_process_seqs] Processed 684014 reads in 105.406 CPU sec, 3.375 real sec
sorting block 58, #reads = 1065835 mem = 536879775 peak = 1173794816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279537, 4, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 252, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (253.58, 134.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (347.05, 165.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 284, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (277.41, 114.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682828 sequences (100000208 bp) in 2.155 CPU sec, 2.320 real sec...
[M::process] read 499264 sequences (73100137 bp) in 1.040 CPU sec, 1.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278176, 6, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 235, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (244.11, 87.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 333, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (349.40, 167.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 287, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (339.82, 183.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1351)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683690 reads in 163.751 CPU sec, 5.254 real sec
[M::mem_process_seqs] Processed 683226 reads in 104.416 CPU sec, 3.451 real sec
sorting block 59, #reads = 1066462 mem = 536881439 peak = 1173794816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 278606, 5, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 220, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (276.17, 184.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 336, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (353.08, 170.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 255, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (311.90, 180.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682828 reads in 87.278 CPU sec, 2.991 real sec
sorting block 60, #reads = 1066162 mem = 536880607 peak = 1173794816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 203888, 6, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 236, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (282.09, 165.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 335, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (354.15, 172.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 308, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 522)
[M::mem_pestat] mean and std.dev: (297.04, 85.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 499264 reads in 80.692 CPU sec, 2.837 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 65310962
overall: 70163369984 mem 9559.667 user 343.242 sys 333.719 real
sorting block 61, #reads = 543531 mem = 273746562 peak = 1173794816
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 333.762 sec; CPU: 9902.918 sec
uniq header count 1
merging 62 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3975294356
output reads: 65695069
bam_mem_sort: 62 calls 22.199 user 0.227 sys 22.583 real
bam_write: 62 calls 50.514 user 0.939 sys 51.957 real
execute: 1 calls 12.601 user 0.628 sys 356.089 real
merge_files: 1 calls 12.575 user 0.616 sys 21.649 real
parse_chunk: 985 calls 30.451 user 8.118 sys 39.314 real
read_chunk: 2659 calls 24.718 user 23.999 sys 268.564 real
sort_block: 1 calls 19.150 user 23.443 sys 260.359 real
write_chunk: 1672 calls 12.170 user 0.491 sys 20.903 real
overall: 3011948544 mem 809.821 user 41.462 sys 356.110 real
ls: cannot access '*.cram': No such file or directory