Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/208a13d31fde01c9b4b30e591fede0/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/93c552ba4a292c63ff0f794aa3aa8d/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/93c552ba4a292c63ff0f794aa3aa8d/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/208a13d31fde01c9b4b30e591fede0/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1363.730 CPU sec, 465.971 real sec
[M::process] read 698132 sequences (100000221 bp) in 0.987 CPU sec, 0.990 real sec...
[M::process] read 696540 sequences (100000093 bp) in 1.384 CPU sec, 1.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290638, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.72, 122.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698900 sequences (100000083 bp) in 1.353 CPU sec, 1.365 real sec...
[M::process] read 699216 sequences (100000140 bp) in 1.389 CPU sec, 1.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290387, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (259.84, 125.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698132 reads in 146.689 CPU sec, 4.638 real sec
[M::mem_process_seqs] Processed 696540 reads in 84.290 CPU sec, 2.688 real sec
sorting block 0, #reads = 1092624 mem = 536878274 peak = 602542080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290680, 6, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.01, 121.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 243, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296)
[M::mem_pestat] mean and std.dev: (290.00, 169.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1667)
[M::mem_pestat] skip orientation RR
[M::process] read 698384 sequences (100000112 bp) in 1.943 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291108, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.49, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (338, 418, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (106, 686)
[M::mem_pestat] mean and std.dev: (341.50, 123.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 696612 sequences (100000011 bp) in 1.370 CPU sec, 1.364 real sec...
[M::mem_process_seqs] Processed 698900 reads in 127.904 CPU sec, 4.060 real sec
[M::mem_process_seqs] Processed 699216 reads in 84.289 CPU sec, 2.674 real sec
sorting block 1, #reads = 1094041 mem = 536881718 peak = 736235520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290739, 5, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (190.70, 71.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.42, 123.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698900 sequences (100000197 bp) in 1.764 CPU sec, 1.805 real sec...
[M::process] read 697992 sequences (100000245 bp) in 1.382 CPU sec, 1.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290054, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 275, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (220.78, 55.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (259.44, 126.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698384 reads in 127.262 CPU sec, 4.043 real sec
[M::mem_process_seqs] Processed 696612 reads in 83.295 CPU sec, 2.648 real sec
sorting block 2, #reads = 1094193 mem = 536881329 peak = 883879936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291738, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.91, 121.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697286 sequences (100000239 bp) in 1.976 CPU sec, 2.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290988, 7, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (252.95, 122.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 217, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544)
[M::mem_pestat] mean and std.dev: (238.93, 92.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] skip orientation RR
[M::process] read 696002 sequences (100000175 bp) in 1.440 CPU sec, 1.445 real sec...
[M::mem_process_seqs] Processed 698900 reads in 127.559 CPU sec, 4.047 real sec
sorting block 3, #reads = 1093211 mem = 536880078 peak = 886423552
[M::mem_process_seqs] Processed 697992 reads in 83.159 CPU sec, 2.640 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290591, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 167, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (243.50, 147.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 4, #reads = 1094316 mem = 536879478 peak = 886423552
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.18, 122.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 263, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (323.09, 142.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697050 sequences (100000248 bp) in 1.988 CPU sec, 2.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289968, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 219, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (264.18, 104.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.57, 122.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697286 reads in 128.831 CPU sec, 4.096 real sec
[M::process] read 696234 sequences (100000059 bp) in 1.460 CPU sec, 1.467 real sec...
[M::mem_process_seqs] Processed 696002 reads in 84.648 CPU sec, 2.679 real sec
sorting block 5, #reads = 1092919 mem = 536883880 peak = 886423552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290363, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.89, 120.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696486 sequences (100000119 bp) in 2.401 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290517, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (255.19, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697050 reads in 128.914 CPU sec, 4.096 real sec
[M::mem_process_seqs] Processed 696234 reads in 85.115 CPU sec, 2.690 real sec
[M::process] read 694678 sequences (100000085 bp) in 1.821 CPU sec, 1.896 real sec...
sorting block 6, #reads = 1092561 mem = 536878919 peak = 886423552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290611, 10, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 206, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (267.55, 179.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.76, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (280, 884, 1207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3061)
[M::mem_pestat] mean and std.dev: (614.62, 417.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3988)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 377, 877)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2283)
[M::mem_pestat] mean and std.dev: (410.56, 279.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2986)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696500 sequences (100000158 bp) in 2.103 CPU sec, 2.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289776, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (280, 380, 906)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2158)
[M::mem_pestat] mean and std.dev: (505.91, 345.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2784)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 237, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (259.68, 123.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696486 reads in 127.004 CPU sec, 4.060 real sec
[M::mem_process_seqs] Processed 694678 reads in 83.334 CPU sec, 2.638 real sec
sorting block 7, #reads = 1093418 mem = 536880055 peak = 886423552
[M::process] read 695592 sequences (100000281 bp) in 2.143 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290322, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.33, 119.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696496 sequences (100000233 bp) in 2.300 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289922, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (254.90, 120.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696500 reads in 128.678 CPU sec, 4.119 real sec
sorting block 8, #reads = 1093296 mem = 536881605 peak = 886423552
[M::mem_process_seqs] Processed 695592 reads in 85.038 CPU sec, 2.702 real sec
[M::process] read 696072 sequences (100000294 bp) in 2.401 CPU sec, 2.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290059, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.72, 120.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1092244 mem = 536880526 peak = 886423552
[M::process] read 697710 sequences (100000293 bp) in 1.792 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290108, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 223, 979)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2491)
[M::mem_pestat] mean and std.dev: (692.09, 815.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3952)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (256.21, 122.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 570)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226)
[M::mem_pestat] mean and std.dev: (291.38, 131.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1554)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696496 reads in 129.408 CPU sec, 4.137 real sec
[M::mem_process_seqs] Processed 696072 reads in 85.675 CPU sec, 2.715 real sec
[M::process] read 698154 sequences (100000181 bp) in 1.878 CPU sec, 1.923 real sec...
sorting block 10, #reads = 1091539 mem = 536877818 peak = 886423552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290959, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.47, 122.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 212, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 430)
[M::mem_pestat] mean and std.dev: (210.70, 48.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 511)
[M::mem_pestat] skip orientation RR
[M::process] read 697898 sequences (100000083 bp) in 1.959 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290366, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (253.69, 121.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697710 reads in 128.825 CPU sec, 4.095 real sec
[M::mem_process_seqs] Processed 698154 reads in 84.068 CPU sec, 2.654 real sec
[M::process] read 698130 sequences (100000125 bp) in 1.721 CPU sec, 1.763 real sec...
sorting block 11, #reads = 1092176 mem = 536878457 peak = 909209600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290297, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 231, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (255.51, 123.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698240 sequences (100000132 bp) in 2.078 CPU sec, 2.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291001, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.62, 123.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697898 reads in 125.963 CPU sec, 4.027 real sec
[M::mem_process_seqs] Processed 698130 reads in 82.752 CPU sec, 2.615 real sec
sorting block 12, #reads = 1093678 mem = 536878574 peak = 909209600
[M::process] read 696612 sequences (100000103 bp) in 1.774 CPU sec, 1.809 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290679, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 287, 2334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6662)
[M::mem_pestat] mean and std.dev: (958.88, 971.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8826)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.40, 122.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 13, #reads = 1094457 mem = 536878547 peak = 951873536
[M::process] read 698130 sequences (100000089 bp) in 2.016 CPU sec, 2.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290649, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (257.73, 123.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 921, 1102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2984)
[M::mem_pestat] mean and std.dev: (811.75, 828.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4124)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698240 reads in 123.225 CPU sec, 3.922 real sec
[M::mem_process_seqs] Processed 696612 reads in 80.281 CPU sec, 2.538 real sec
[M::process] read 697864 sequences (100000119 bp) in 1.768 CPU sec, 1.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291080, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.26, 121.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 169, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (223.89, 219.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 14, #reads = 1094648 mem = 536879759 peak = 951873536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290600, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 274, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (293.75, 143.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1538)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.17, 122.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697618 sequences (100000214 bp) in 2.347 CPU sec, 2.557 real sec...
[M::mem_process_seqs] Processed 698130 reads in 121.125 CPU sec, 3.889 real sec
[M::mem_process_seqs] Processed 697864 reads in 80.768 CPU sec, 2.561 real sec
sorting block 15, #reads = 1094831 mem = 536883277 peak = 951873536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291121, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (254.07, 122.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 694968 sequences (100000046 bp) in 2.452 CPU sec, 2.676 real sec...
[M::mem_process_seqs] Processed 697618 reads in 69.295 CPU sec, 2.302 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 289906, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 238, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (261.46, 125.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 883, 1918)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5378)
[M::mem_pestat] mean and std.dev: (874.25, 792.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7108)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694968 reads in 68.557 CPU sec, 2.265 real sec
sorting block 16, #reads = 1094970 mem = 536883027 peak = 951873536
[M::process] read 696352 sequences (100000161 bp) in 2.560 CPU sec, 2.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290338, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 385, 1873)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5255)
[M::mem_pestat] mean and std.dev: (784.69, 918.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6946)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.05, 121.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696352 reads in 69.939 CPU sec, 2.303 real sec
[M::process] read 696980 sequences (100000121 bp) in 2.860 CPU sec, 3.157 real sec...
sorting block 17, #reads = 1093421 mem = 536877488 peak = 951873536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290568, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.75, 121.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696980 reads in 69.453 CPU sec, 2.253 real sec
[M::process] read 697250 sequences (100000055 bp) in 2.812 CPU sec, 3.085 real sec...
sorting block 18, #reads = 1093314 mem = 536877991 peak = 951873536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290801, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.70, 120.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695952 sequences (100000238 bp) in 1.865 CPU sec, 1.971 real sec...
[M::mem_process_seqs] Processed 697250 reads in 69.181 CPU sec, 2.229 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290403, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 346, 3468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9982)
[M::mem_pestat] mean and std.dev: (1607.70, 1856.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13239)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.89, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 2059, 4264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12322)
[M::mem_pestat] mean and std.dev: (2678.30, 3072.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16351)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695952 reads in 68.649 CPU sec, 2.217 real sec
[M::process] read 695900 sequences (100000250 bp) in 2.466 CPU sec, 2.679 real sec...
sorting block 19, #reads = 1093841 mem = 536878471 peak = 951873536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290244, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.06, 120.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695900 reads in 68.549 CPU sec, 2.219 real sec
[M::process] read 696862 sequences (100000167 bp) in 2.135 CPU sec, 2.308 real sec...
sorting block 20, #reads = 1092490 mem = 536883607 peak = 951873536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290349, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.12, 119.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696862 reads in 68.770 CPU sec, 2.233 real sec
[M::process] read 696732 sequences (100000058 bp) in 2.490 CPU sec, 2.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290049, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.60, 119.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697520 sequences (100000012 bp) in 1.862 CPU sec, 2.016 real sec...
[M::mem_process_seqs] Processed 696732 reads in 68.225 CPU sec, 2.211 real sec
sorting block 21, #reads = 1093128 mem = 536879877 peak = 951873536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290581, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.63, 120.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698070 sequences (100000172 bp) in 2.261 CPU sec, 2.436 real sec...
[M::mem_process_seqs] Processed 697520 reads in 70.241 CPU sec, 2.277 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290464, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.56, 120.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 206, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (217.22, 72.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1092275 mem = 536875271 peak = 951873536
[M::mem_process_seqs] Processed 698070 reads in 71.968 CPU sec, 2.327 real sec
[M::process] read 699210 sequences (100000065 bp) in 2.374 CPU sec, 2.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291066, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.49, 119.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699210 reads in 70.918 CPU sec, 2.297 real sec
[M::process] read 699132 sequences (100000274 bp) in 2.593 CPU sec, 2.936 real sec...
sorting block 23, #reads = 1092834 mem = 536880479 peak = 951873536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291030, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (251.85, 120.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698060 sequences (100000231 bp) in 1.911 CPU sec, 2.039 real sec...
[M::mem_process_seqs] Processed 699132 reads in 70.269 CPU sec, 2.265 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291213, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.32, 120.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 147, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (171.89, 81.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 626)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698060 reads in 68.922 CPU sec, 2.230 real sec
[M::process] read 696814 sequences (100000245 bp) in 2.252 CPU sec, 2.455 real sec...
sorting block 24, #reads = 1093607 mem = 536879131 peak = 951873536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290760, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 236, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.65, 126.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696814 reads in 69.217 CPU sec, 2.251 real sec
[M::process] read 697258 sequences (100000172 bp) in 2.209 CPU sec, 2.403 real sec...
sorting block 25, #reads = 1093487 mem = 536885380 peak = 951873536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291068, 11, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (256.70, 123.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 272, 981)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2525)
[M::mem_pestat] mean and std.dev: (569.60, 648.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3297)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 698692 sequences (100000295 bp) in 1.804 CPU sec, 1.845 real sec...
[M::mem_process_seqs] Processed 697258 reads in 68.148 CPU sec, 2.203 real sec
[M::process] read 697834 sequences (100000043 bp) in 1.774 CPU sec, 1.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291034, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.41, 121.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697818 sequences (100000012 bp) in 1.320 CPU sec, 1.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290763, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.86, 121.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698692 reads in 119.539 CPU sec, 3.759 real sec
sorting block 26, #reads = 1092844 mem = 536878989 peak = 951873536
[M::mem_process_seqs] Processed 697834 reads in 78.329 CPU sec, 2.467 real sec
[M::process] read 696920 sequences (100000124 bp) in 1.558 CPU sec, 1.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290632, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 237, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (238.67, 128.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1385)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.81, 121.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 27, #reads = 1093622 mem = 536877899 peak = 998354944
[M::process] read 695034 sequences (100000189 bp) in 1.955 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290357, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (255.96, 122.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 281, 846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2088)
[M::mem_pestat] mean and std.dev: (427.60, 311.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2709)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697818 reads in 122.624 CPU sec, 3.914 real sec
[M::mem_process_seqs] Processed 696920 reads in 80.337 CPU sec, 2.538 real sec
[M::process] read 697030 sequences (100000118 bp) in 1.845 CPU sec, 1.937 real sec...
sorting block 28, #reads = 1092641 mem = 536879217 peak = 998354944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290191, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 238, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (261.76, 124.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696580 sequences (100000199 bp) in 2.165 CPU sec, 2.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290940, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 479, 765)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1951)
[M::mem_pestat] mean and std.dev: (451.64, 265.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2544)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (254.62, 121.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 695034 reads in 121.579 CPU sec, 3.882 real sec
[M::mem_process_seqs] Processed 697030 reads in 81.309 CPU sec, 2.577 real sec
sorting block 29, #reads = 1093029 mem = 536877977 peak = 998354944
[M::process] read 696784 sequences (100000293 bp) in 1.878 CPU sec, 1.938 real sec...
[M::process] read 697100 sequences (100000203 bp) in 2.280 CPU sec, 2.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290361, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (341.55, 155.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.81, 120.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696784 reads in 68.748 CPU sec, 2.178 real sec
sorting block 30, #reads = 1093717 mem = 536880108 peak = 998354944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290468, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 281, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (256.62, 66.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.48, 121.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697274 sequences (100000274 bp) in 1.888 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290149, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (250.98, 118.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696580 reads in 189.622 CPU sec, 6.034 real sec
[M::mem_process_seqs] Processed 697100 reads in 93.976 CPU sec, 2.981 real sec
[M::process] read 696864 sequences (100000190 bp) in 1.785 CPU sec, 1.834 real sec...
sorting block 31, #reads = 1092824 mem = 536878704 peak = 998354944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290715, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.35, 118.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 289, 1266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3282)
[M::mem_pestat] mean and std.dev: (578.36, 729.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4290)
[M::mem_pestat] skip orientation RR
[M::process] read 696922 sequences (100000160 bp) in 1.884 CPU sec, 1.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290342, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.24, 119.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697274 reads in 119.651 CPU sec, 3.810 real sec
[M::mem_process_seqs] Processed 696864 reads in 78.953 CPU sec, 2.498 real sec
sorting block 32, #reads = 1093371 mem = 536879121 peak = 998354944
[M::process] read 696944 sequences (100000157 bp) in 1.960 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290444, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.78, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697386 sequences (100000009 bp) in 2.289 CPU sec, 2.483 real sec...
[M::process] read 696148 sequences (100000268 bp) in 1.310 CPU sec, 1.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290359, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (417, 1741, 1762)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4452)
[M::mem_pestat] mean and std.dev: (1086.92, 749.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.59, 120.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 317, 859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2037)
[M::mem_pestat] mean and std.dev: (443.90, 343.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2626)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697386 reads in 64.700 CPU sec, 2.096 real sec
sorting block 33, #reads = 1092865 mem = 536878741 peak = 998354944
[M::mem_process_seqs] Processed 696944 reads in 133.082 CPU sec, 4.870 real sec
sorting block 34, #reads = 1092726 mem = 536878253 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290042, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (257.18, 121.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 298, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (272.73, 172.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696148 reads in 67.048 CPU sec, 2.193 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290980, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.90, 120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 179, 856)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2248)
[M::mem_pestat] mean and std.dev: (421.08, 328.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2944)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696922 reads in 267.576 CPU sec, 9.398 real sec
[M::process] read 696520 sequences (100000224 bp) in 2.661 CPU sec, 2.918 real sec...
sorting block 35, #reads = 1092184 mem = 536879803 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290122, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.22, 121.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696520 reads in 66.330 CPU sec, 2.202 real sec
[M::process] read 696998 sequences (100000198 bp) in 2.668 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290473, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.58, 119.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1093504 mem = 536880049 peak = 1094934528
[M::mem_process_seqs] Processed 696998 reads in 67.641 CPU sec, 2.207 real sec
[M::process] read 694906 sequences (100000034 bp) in 2.614 CPU sec, 2.914 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289975, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 241, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (264.42, 126.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694906 reads in 66.698 CPU sec, 2.165 real sec
sorting block 37, #reads = 1093493 mem = 536878899 peak = 1094934528
[M::process] read 697906 sequences (100000212 bp) in 2.529 CPU sec, 2.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291067, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.61, 122.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698490 sequences (100000105 bp) in 1.872 CPU sec, 1.974 real sec...
[M::mem_process_seqs] Processed 697906 reads in 67.496 CPU sec, 2.183 real sec
sorting block 38, #reads = 1093305 mem = 536880967 peak = 1094934528
[M::process] read 698498 sequences (100000023 bp) in 1.895 CPU sec, 1.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291057, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.37, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698500 sequences (100000088 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291090, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 266, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (216.88, 107.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.49, 120.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698490 reads in 122.764 CPU sec, 3.884 real sec
[M::mem_process_seqs] Processed 698498 reads in 80.821 CPU sec, 2.548 real sec
[M::process] read 696576 sequences (100000088 bp) in 2.086 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291185, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.55, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1094099 mem = 536878782 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290636, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 772, 1600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4502)
[M::mem_pestat] mean and std.dev: (679.67, 588.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5953)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (257.48, 123.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698500 reads in 122.443 CPU sec, 3.932 real sec
[M::process] read 696476 sequences (100000001 bp) in 2.422 CPU sec, 2.618 real sec...
[M::mem_process_seqs] Processed 696576 reads in 81.164 CPU sec, 2.584 real sec
sorting block 40, #reads = 1094708 mem = 536879398 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290499, 15, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 211, 2973)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8551)
[M::mem_pestat] mean and std.dev: (982.33, 1177.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11340)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (257.91, 123.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 602, 680)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1786)
[M::mem_pestat] mean and std.dev: (409.77, 278.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2339)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (345, 434, 1651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4263)
[M::mem_pestat] mean and std.dev: (721.18, 585.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5569)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696476 reads in 69.054 CPU sec, 2.273 real sec
[M::process] read 697298 sequences (100000018 bp) in 2.425 CPU sec, 2.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290207, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.71, 121.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1092640 mem = 536880839 peak = 1094934528
[M::mem_process_seqs] Processed 697298 reads in 68.941 CPU sec, 2.265 real sec
[M::process] read 696160 sequences (100000217 bp) in 2.599 CPU sec, 2.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290344, 10, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 716, 716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516)
[M::mem_pestat] mean and std.dev: (493.40, 253.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1916)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (258.32, 123.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (90, 542, 1256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3588)
[M::mem_pestat] mean and std.dev: (523.89, 517.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4754)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 696160 reads in 68.322 CPU sec, 2.219 real sec
sorting block 42, #reads = 1093014 mem = 536880502 peak = 1094934528
[M::process] read 696758 sequences (100000101 bp) in 2.803 CPU sec, 3.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290348, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.15, 121.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696758 reads in 67.941 CPU sec, 2.196 real sec
[M::process] read 696922 sequences (100000241 bp) in 2.045 CPU sec, 2.222 real sec...
sorting block 43, #reads = 1092190 mem = 536876597 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290328, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.59, 118.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696922 reads in 68.944 CPU sec, 2.236 real sec
[M::process] read 696850 sequences (100000040 bp) in 2.542 CPU sec, 2.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289684, 9, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 343, 3873)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11163)
[M::mem_pestat] mean and std.dev: (1480.91, 2040.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14808)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.41, 118.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696970 sequences (100000021 bp) in 1.835 CPU sec, 1.934 real sec...
[M::mem_process_seqs] Processed 696850 reads in 67.855 CPU sec, 2.181 real sec
sorting block 44, #reads = 1091811 mem = 536877039 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290526, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.06, 119.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696970 reads in 69.949 CPU sec, 2.258 real sec
[M::process] read 696896 sequences (100000030 bp) in 2.519 CPU sec, 2.714 real sec...
sorting block 45, #reads = 1091809 mem = 536881415 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290538, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.61, 120.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696896 reads in 68.370 CPU sec, 2.209 real sec
[M::process] read 700298 sequences (100000272 bp) in 2.321 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291405, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.19, 116.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702010 sequences (100000073 bp) in 2.096 CPU sec, 2.246 real sec...
[M::mem_process_seqs] Processed 700298 reads in 71.426 CPU sec, 2.304 real sec
sorting block 46, #reads = 1093261 mem = 536878500 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292441, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (244.23, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 311, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 561)
[M::mem_pestat] mean and std.dev: (303.10, 86.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 672)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702010 reads in 71.999 CPU sec, 2.328 real sec
[M::process] read 702212 sequences (100000160 bp) in 2.395 CPU sec, 2.574 real sec...
sorting block 47, #reads = 1093586 mem = 536879119 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292312, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (244.18, 119.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 392, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278)
[M::mem_pestat] mean and std.dev: (434.40, 236.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1617)
[M::mem_pestat] skip orientation RR
[M::process] read 702872 sequences (100000172 bp) in 2.140 CPU sec, 2.290 real sec...
[M::mem_process_seqs] Processed 702212 reads in 71.146 CPU sec, 2.295 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292588, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 190, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 439)
[M::mem_pestat] mean and std.dev: (185.20, 50.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 522)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.88, 117.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702872 reads in 70.846 CPU sec, 2.303 real sec
[M::process] read 702392 sequences (100000251 bp) in 2.363 CPU sec, 2.568 real sec...
sorting block 48, #reads = 1094113 mem = 536877412 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292268, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.02, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 411, 1182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120)
[M::mem_pestat] mean and std.dev: (679.64, 567.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4089)
[M::mem_pestat] skip orientation RR
[M::process] read 699404 sequences (100000267 bp) in 1.980 CPU sec, 2.093 real sec...
[M::mem_process_seqs] Processed 702392 reads in 71.067 CPU sec, 2.294 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291630, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 225, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (251.97, 123.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1094765 mem = 536877297 peak = 1094934528
[M::mem_process_seqs] Processed 699404 reads in 71.347 CPU sec, 2.298 real sec
[M::process] read 702584 sequences (100000245 bp) in 2.346 CPU sec, 2.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292625, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 522, 971)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545)
[M::mem_pestat] mean and std.dev: (543.80, 429.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3332)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.43, 116.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 268, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1375)
[M::mem_pestat] mean and std.dev: (381.55, 310.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1775)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 701478 sequences (100000028 bp) in 2.010 CPU sec, 2.096 real sec...
[M::mem_process_seqs] Processed 702584 reads in 73.868 CPU sec, 2.382 real sec
sorting block 50, #reads = 1093419 mem = 536877839 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292346, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.43, 119.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698832 sequences (100000040 bp) in 2.302 CPU sec, 2.474 real sec...
[M::mem_process_seqs] Processed 701478 reads in 71.252 CPU sec, 2.297 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291361, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.50, 123.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698832 reads in 70.567 CPU sec, 2.293 real sec
sorting block 51, #reads = 1093734 mem = 536880617 peak = 1094934528
[M::process] read 698540 sequences (100000235 bp) in 2.433 CPU sec, 2.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291403, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.75, 123.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697604 sequences (100000088 bp) in 2.101 CPU sec, 2.265 real sec...
[M::mem_process_seqs] Processed 698540 reads in 70.170 CPU sec, 2.274 real sec
sorting block 52, #reads = 1092428 mem = 536884619 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290500, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.22, 122.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 273, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (237.62, 67.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697604 reads in 70.179 CPU sec, 2.271 real sec
[M::process] read 697434 sequences (100000176 bp) in 2.559 CPU sec, 2.801 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290629, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 305, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1687)
[M::mem_pestat] mean and std.dev: (394.40, 318.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2196)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.24, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697434 reads in 70.605 CPU sec, 2.281 real sec
[M::process] read 696800 sequences (100000113 bp) in 2.220 CPU sec, 2.372 real sec...
sorting block 53, #reads = 1091102 mem = 536881814 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290721, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.81, 123.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696800 reads in 70.900 CPU sec, 2.299 real sec
[M::process] read 697100 sequences (100000097 bp) in 2.477 CPU sec, 2.713 real sec...
sorting block 54, #reads = 1091036 mem = 536876906 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290506, 10, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 179, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (217.11, 91.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.25, 121.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 504, 631)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1553)
[M::mem_pestat] mean and std.dev: (380.80, 290.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2014)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (303, 373, 859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1971)
[M::mem_pestat] mean and std.dev: (484.90, 323.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2527)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697114 sequences (100000123 bp) in 1.838 CPU sec, 1.886 real sec...
[M::mem_process_seqs] Processed 697100 reads in 71.656 CPU sec, 2.306 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290409, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.98, 121.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697482 sequences (100000033 bp) in 2.300 CPU sec, 2.476 real sec...
[M::mem_process_seqs] Processed 697114 reads in 70.662 CPU sec, 2.299 real sec
sorting block 55, #reads = 1090001 mem = 536877601 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290887, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.99, 121.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697490 sequences (100000114 bp) in 2.022 CPU sec, 2.172 real sec...
[M::mem_process_seqs] Processed 697482 reads in 71.907 CPU sec, 2.330 real sec
sorting block 56, #reads = 1091071 mem = 536880537 peak = 1094934528
[M::process] read 703310 sequences (100000140 bp) in 2.010 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290369, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.77, 121.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704512 sequences (100000015 bp) in 1.440 CPU sec, 1.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292201, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.74, 116.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697490 reads in 139.575 CPU sec, 4.384 real sec
[M::process] read 703714 sequences (100000191 bp) in 1.468 CPU sec, 1.475 real sec...
[M::mem_process_seqs] Processed 703310 reads in 100.545 CPU sec, 3.169 real sec
sorting block 57, #reads = 1088938 mem = 536879454 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293035, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (238.59, 115.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 1405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3803)
[M::mem_pestat] mean and std.dev: (545.67, 583.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5002)
[M::mem_pestat] skip orientation RR
[M::process] read 703240 sequences (100000053 bp) in 2.189 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293014, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 701400 sequences (100000153 bp) in 1.540 CPU sec, 1.535 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (239.80, 115.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704512 reads in 158.049 CPU sec, 5.031 real sec
[M::mem_process_seqs] Processed 703714 reads in 102.019 CPU sec, 3.245 real sec
sorting block 58, #reads = 1087465 mem = 536878061 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293093, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.16, 115.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701948 sequences (100000150 bp) in 2.277 CPU sec, 2.441 real sec...
[M::process] read 702502 sequences (100000078 bp) in 1.520 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292503, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.38, 117.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703240 reads in 152.120 CPU sec, 4.827 real sec
[M::mem_process_seqs] Processed 701400 reads in 98.095 CPU sec, 3.119 real sec
sorting block 59, #reads = 1089494 mem = 536887416 peak = 1094934528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292519, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.04, 115.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701006 sequences (100000298 bp) in 2.074 CPU sec, 2.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292681, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.00, 116.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698768 sequences (100000183 bp) in 1.620 CPU sec, 1.618 real sec...
[M::mem_process_seqs] Processed 701948 reads in 148.842 CPU sec, 4.746 real sec
sorting block 60, #reads = 1090938 mem = 536882027 peak = 1105465344
[M::mem_process_seqs] Processed 702502 reads in 96.744 CPU sec, 3.069 real sec
sorting block 61, #reads = 1091210 mem = 536878457 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292474, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.75, 119.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699136 sequences (100000216 bp) in 2.171 CPU sec, 2.213 real sec...
[M::process] read 698396 sequences (100000151 bp) in 1.550 CPU sec, 1.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291785, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (254.02, 124.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701006 reads in 148.348 CPU sec, 4.709 real sec
[M::mem_process_seqs] Processed 698768 reads in 94.020 CPU sec, 2.988 real sec
sorting block 62, #reads = 1090511 mem = 536883324 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291104, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.70, 121.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697506 sequences (100000080 bp) in 1.950 CPU sec, 2.006 real sec...
[M::process] read 696976 sequences (100000101 bp) in 1.570 CPU sec, 1.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290662, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.61, 121.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 547, 5320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15482)
[M::mem_pestat] mean and std.dev: (2021.50, 2345.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20563)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699136 reads in 143.778 CPU sec, 4.564 real sec
[M::mem_process_seqs] Processed 698396 reads in 92.881 CPU sec, 2.954 real sec
sorting block 63, #reads = 1091233 mem = 536876807 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290238, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 347, 860)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2184)
[M::mem_pestat] mean and std.dev: (334.60, 218.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2846)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.28, 122.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 233, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (224.80, 79.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698362 sequences (100000025 bp) in 2.214 CPU sec, 2.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290695, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 698316 sequences (100000179 bp) in 1.580 CPU sec, 1.573 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (255.75, 124.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697506 reads in 142.558 CPU sec, 4.517 real sec
sorting block 64, #reads = 1089881 mem = 536880240 peak = 1166016512
[M::mem_process_seqs] Processed 696976 reads in 91.686 CPU sec, 2.911 real sec
sorting block 65, #reads = 1090146 mem = 536879676 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291008, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.07, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698382 sequences (100000280 bp) in 2.049 CPU sec, 2.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290869, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.49, 121.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702066 sequences (100000032 bp) in 1.640 CPU sec, 1.642 real sec...
[M::mem_process_seqs] Processed 698362 reads in 143.452 CPU sec, 4.550 real sec
[M::mem_process_seqs] Processed 698316 reads in 92.672 CPU sec, 2.937 real sec
sorting block 66, #reads = 1091478 mem = 536886257 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291234, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.38, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702982 sequences (100000176 bp) in 2.235 CPU sec, 2.280 real sec...
[M::process] read 701610 sequences (100000227 bp) in 1.560 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291771, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (242.04, 117.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698382 reads in 150.700 CPU sec, 4.778 real sec
[M::mem_process_seqs] Processed 702066 reads in 99.855 CPU sec, 3.170 real sec
sorting block 67, #reads = 1089367 mem = 536880329 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 293046, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (242.10, 117.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701160 sequences (100000270 bp) in 1.975 CPU sec, 2.019 real sec...
[M::process] read 698118 sequences (100000136 bp) in 1.560 CPU sec, 1.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292214, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (244.77, 118.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702982 reads in 137.762 CPU sec, 4.362 real sec
sorting block 68, #reads = 1087753 mem = 536878774 peak = 1166016512
[M::mem_process_seqs] Processed 701610 reads in 87.386 CPU sec, 2.771 real sec
sorting block 69, #reads = 1094916 mem = 536881596 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292156, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.33, 120.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701606 sequences (100000069 bp) in 2.033 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290915, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (254.99, 123.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 304, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (246.38, 94.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RR
[M::process] read 702578 sequences (100000053 bp) in 1.620 CPU sec, 1.623 real sec...
[M::mem_process_seqs] Processed 701160 reads in 136.588 CPU sec, 4.324 real sec
[M::mem_process_seqs] Processed 698118 reads in 87.634 CPU sec, 2.771 real sec
sorting block 70, #reads = 1093204 mem = 536880507 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292068, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.53, 116.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701086 sequences (100000056 bp) in 2.012 CPU sec, 2.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292210, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 236, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (238.11, 111.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.09, 115.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700800 sequences (100000178 bp) in 1.600 CPU sec, 1.600 real sec...
[M::mem_process_seqs] Processed 701606 reads in 139.223 CPU sec, 4.411 real sec
[M::mem_process_seqs] Processed 702578 reads in 89.849 CPU sec, 2.838 real sec
sorting block 71, #reads = 1094259 mem = 536879943 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291924, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (319, 1268, 2798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7756)
[M::mem_pestat] mean and std.dev: (1624.27, 1284.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10235)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (243.85, 116.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698856 sequences (100000075 bp) in 2.161 CPU sec, 2.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291899, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.60, 118.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700172 sequences (100000257 bp) in 1.760 CPU sec, 1.758 real sec...
[M::mem_process_seqs] Processed 701086 reads in 138.310 CPU sec, 4.372 real sec
[M::mem_process_seqs] Processed 700800 reads in 87.933 CPU sec, 2.767 real sec
sorting block 72, #reads = 1093886 mem = 536882312 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291551, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 233, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (220.44, 104.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (253.64, 123.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700076 sequences (100000041 bp) in 1.997 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291607, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 592, 694)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1680)
[M::mem_pestat] mean and std.dev: (416.73, 239.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2173)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.21, 118.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699296 sequences (100000269 bp) in 1.600 CPU sec, 1.600 real sec...
[M::mem_process_seqs] Processed 698856 reads in 136.856 CPU sec, 4.337 real sec
sorting block 73, #reads = 1094124 mem = 536878651 peak = 1166016512
[M::mem_process_seqs] Processed 700172 reads in 87.052 CPU sec, 2.745 real sec
sorting block 74, #reads = 1093001 mem = 536880626 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291808, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.64, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698866 sequences (100000142 bp) in 2.090 CPU sec, 2.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291356, 4, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 699270 sequences (100000043 bp) in 1.620 CPU sec, 1.616 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (249.80, 121.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 287, 3395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9689)
[M::mem_pestat] mean and std.dev: (1414.60, 1888.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12836)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700076 reads in 139.484 CPU sec, 4.414 real sec
[M::mem_process_seqs] Processed 699296 reads in 89.835 CPU sec, 2.840 real sec
sorting block 75, #reads = 1092983 mem = 536880033 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291050, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.95, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699072 sequences (100000205 bp) in 2.217 CPU sec, 2.253 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291235, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.19, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 264, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (264.89, 96.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RR
[M::process] read 699446 sequences (100000119 bp) in 1.660 CPU sec, 1.663 real sec...
[M::mem_process_seqs] Processed 698866 reads in 143.339 CPU sec, 4.534 real sec
[M::mem_process_seqs] Processed 699270 reads in 92.317 CPU sec, 2.914 real sec
sorting block 76, #reads = 1091251 mem = 536877494 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290900, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.55, 120.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 305, 650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1638)
[M::mem_pestat] mean and std.dev: (350.42, 223.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2132)
[M::mem_pestat] skip orientation RR
[M::process] read 703904 sequences (100000159 bp) in 2.118 CPU sec, 2.158 real sec...
[M::process] read 703988 sequences (100000264 bp) in 1.650 CPU sec, 1.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291245, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 378, 755)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1911)
[M::mem_pestat] mean and std.dev: (534.77, 465.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2489)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (245.30, 117.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 315, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (327.23, 173.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699072 reads in 145.896 CPU sec, 4.620 real sec
sorting block 77, #reads = 1090540 mem = 536880995 peak = 1166016512
[M::mem_process_seqs] Processed 699446 reads in 94.821 CPU sec, 2.999 real sec
sorting block 78, #reads = 1088903 mem = 536876408 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 292695, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 251, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 340)
[M::mem_pestat] mean and std.dev: (228.45, 32.38)
[M::mem_pestat] low and high boundaries for proper pairs: (82, 383)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.48, 115.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 337, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (263.36, 78.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 704738 sequences (100000230 bp) in 2.103 CPU sec, 2.147 real sec...
[M::process] read 701880 sequences (100000008 bp) in 1.670 CPU sec, 1.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292731, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (241.17, 117.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703904 reads in 163.544 CPU sec, 5.190 real sec
[M::mem_process_seqs] Processed 703988 reads in 104.082 CPU sec, 3.308 real sec
sorting block 79, #reads = 1085095 mem = 536878135 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293527, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (237.16, 114.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703852 sequences (100000218 bp) in 2.139 CPU sec, 2.171 real sec...
[M::process] read 704226 sequences (100000075 bp) in 1.620 CPU sec, 1.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292161, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 363, 602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1492)
[M::mem_pestat] mean and std.dev: (321.08, 195.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1937)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (247.11, 120.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 704738 reads in 154.608 CPU sec, 4.901 real sec
[M::mem_process_seqs] Processed 701880 reads in 97.385 CPU sec, 3.087 real sec
sorting block 80, #reads = 1089485 mem = 536879573 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292572, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (240.73, 117.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703392 sequences (100000117 bp) in 2.074 CPU sec, 2.107 real sec...
[M::process] read 702014 sequences (100000281 bp) in 1.620 CPU sec, 1.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292506, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (237.13, 114.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703852 reads in 148.254 CPU sec, 4.698 real sec
[M::mem_process_seqs] Processed 704226 reads in 94.290 CPU sec, 2.992 real sec
sorting block 81, #reads = 1089349 mem = 536879280 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292522, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.73, 112.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699688 sequences (100000211 bp) in 2.123 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291882, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.22, 115.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 295, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171)
[M::mem_pestat] mean and std.dev: (330.20, 271.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1506)
[M::mem_pestat] skip orientation RR
[M::process] read 700874 sequences (100000024 bp) in 1.610 CPU sec, 1.604 real sec...
[M::mem_process_seqs] Processed 703392 reads in 148.209 CPU sec, 4.718 real sec
sorting block 82, #reads = 1091890 mem = 536877329 peak = 1166016512
[M::mem_process_seqs] Processed 702014 reads in 93.608 CPU sec, 2.950 real sec
sorting block 83, #reads = 1091893 mem = 536879340 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291316, 10, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 285, 695)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689)
[M::mem_pestat] mean and std.dev: (376.50, 244.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2186)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (248.96, 119.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 316, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (263.78, 186.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 700016 sequences (100000262 bp) in 2.099 CPU sec, 2.145 real sec...
[M::process] read 699990 sequences (100000173 bp) in 1.600 CPU sec, 1.598 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292237, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 586, 1395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3847)
[M::mem_pestat] mean and std.dev: (717.08, 602.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5073)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.26, 116.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699688 reads in 143.576 CPU sec, 4.549 real sec
[M::mem_process_seqs] Processed 700874 reads in 90.290 CPU sec, 2.860 real sec
sorting block 84, #reads = 1090875 mem = 536878620 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291695, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (282, 524, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194)
[M::mem_pestat] mean and std.dev: (445.20, 182.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1498)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.29, 118.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699184 sequences (100000205 bp) in 2.015 CPU sec, 2.117 real sec...
[M::process] read 698832 sequences (100000195 bp) in 1.610 CPU sec, 1.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291626, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.75, 118.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 861, 905)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273)
[M::mem_pestat] mean and std.dev: (675.78, 449.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2957)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 700016 reads in 144.699 CPU sec, 4.594 real sec
[M::mem_process_seqs] Processed 699990 reads in 89.125 CPU sec, 2.829 real sec
sorting block 85, #reads = 1092077 mem = 536878644 peak = 1166016512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291532, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.64, 119.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 253, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669)
[M::mem_pestat] mean and std.dev: (350.62, 287.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2185)
[M::mem_pestat] skip orientation RR
[M::process] read 698796 sequences (100000158 bp) in 2.024 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290903, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.06, 118.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 263, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (327.55, 121.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RR
[M::process] read 698640 sequences (100000110 bp) in 1.590 CPU sec, 1.596 real sec...
[M::mem_process_seqs] Processed 699184 reads in 139.873 CPU sec, 4.432 real sec
sorting block 86, #reads = 1092646 mem = 536879582 peak = 1166016512
[M::mem_process_seqs] Processed 698832 reads in 88.985 CPU sec, 2.811 real sec
[M::process] read 245962 sequences (35093840 bp) in 1.137 CPU sec, 1.150 real sec...
sorting block 87, #reads = 1090481 mem = 536880095 peak = 1172070400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291374, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (285, 336, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221)
[M::mem_pestat] mean and std.dev: (443.36, 219.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1533)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.93, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 675, 1093)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2837)
[M::mem_pestat] mean and std.dev: (609.62, 390.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3709)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 102242, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.20, 117.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290710, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (176.67, 70.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.54, 120.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698796 reads in 160.285 CPU sec, 5.164 real sec
[M::mem_process_seqs] Processed 245962 reads in 101.840 CPU sec, 3.287 real sec
[M::mem_process_seqs] Processed 698640 reads in 115.548 CPU sec, 3.752 real sec
sorting block 88, #reads = 1091048 mem = 536879605 peak = 1172070400
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 97368698
overall: 69772455936 mem 11245.912 user 351.851 sys 813.536 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 813.584 sec; CPU: 11597.775 sec
sorting block 89, #reads = 647424 mem = 318929913 peak = 1172070400
uniq header count 1
merging 90 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5229015759
output reads: 97864175
bam_mem_sort: 90 calls 35.865 user 0.610 sys 36.641 real
bam_write: 90 calls 83.137 user 2.248 sys 85.897 real
execute: 1 calls 26.649 user 1.172 sys 844.885 real
merge_files: 1 calls 26.623 user 1.139 sys 30.503 real
parse_chunk: 1434 calls 59.613 user 15.514 sys 76.193 real
read_chunk: 3872 calls 32.795 user 26.229 sys 356.085 real
sort_block: 1 calls 23.874 user 25.881 sys 342.744 real
write_chunk: 2436 calls 26.167 user 0.896 sys 29.528 real
overall: 3055292416 mem 1195.066 user 53.387 sys 844.908 real
ls: cannot access '*.cram': No such file or directory