.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1136_3U6-T1-TDNA-1_A23MHWWLT4_1;L005;0003)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e0/b32c2caecc11a28beaaceb967dc40e/.command.err
Size: 123.1 KB
Task: DAQ:SENTIEON_BWAMEM (1136_3U6-T1-TDNA-1_A23MHWWLT4_1;L005;0003)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 2137.616 CPU sec, 498.756 real sec
[M::process] read 688392 sequences (100000159 bp) in 1.021 CPU sec, 1.029 real sec...
[M::process] read 686020 sequences (100000187 bp) in 1.180 CPU sec, 1.173 real sec...
[M::process] read 685522 sequences (100000195 bp) in 1.309 CPU sec, 1.311 real sec...
[M::process] read 684322 sequences (100000022 bp) in 1.250 CPU sec, 1.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284125, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 268, 1156)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3092)
[M::mem_pestat] mean and std.dev: (644.87, 715.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4060)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 290, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (311.58, 155.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 262, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (242.06, 97.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 667)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283736, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 290, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (330.80, 169.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 305, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (322.91, 158.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 357, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (376.36, 162.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282970, 12, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 247, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (229.00, 75.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 311, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (327.32, 160.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 196, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366)
[M::mem_pestat] mean and std.dev: (156.09, 84.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 495)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 192, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (228.73, 136.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688392 reads in 292.785 CPU sec, 9.365 real sec
[M::mem_process_seqs] Processed 686020 reads in 170.900 CPU sec, 5.555 real sec
[M::mem_process_seqs] Processed 685522 reads in 185.362 CPU sec, 6.063 real sec
sorting block 0, #reads = 1082542 mem = 536877596 peak = 587087872
[M::process] read 683664 sequences (100000163 bp) in 1.971 CPU sec, 2.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283053, 7, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 364, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155)
[M::mem_pestat] mean and std.dev: (393.12, 250.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1476)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (331.90, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (295, 364, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111)
[M::mem_pestat] mean and std.dev: (363.80, 131.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1383)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683400 sequences (100000194 bp) in 1.432 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282839, 2, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 862, 1638)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4438)
[M::mem_pestat] mean and std.dev: (958.86, 861.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5838)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (335.95, 161.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 332, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364)
[M::mem_pestat] mean and std.dev: (397.62, 233.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1752)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682656 sequences (100000025 bp) in 1.220 CPU sec, 1.213 real sec...
[M::mem_process_seqs] Processed 684322 reads in 162.067 CPU sec, 5.179 real sec
sorting block 1, #reads = 1080820 mem = 536880880 peak = 798478336
[M::mem_process_seqs] Processed 683664 reads in 102.193 CPU sec, 3.264 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282899, 9, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 275, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (346.92, 212.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1362)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (338.13, 163.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 303, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (77, 532)
[M::mem_pestat] mean and std.dev: (295.55, 67.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 623)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 2, #reads = 1081123 mem = 536880006 peak = 856084480
[M::process] read 682068 sequences (100000231 bp) in 2.594 CPU sec, 2.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282538, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 304, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (349.92, 170.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1370)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (339.07, 161.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (291, 402, 757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689)
[M::mem_pestat] mean and std.dev: (483.90, 331.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2155)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682050 sequences (100000149 bp) in 1.575 CPU sec, 1.591 real sec...
[M::mem_process_seqs] Processed 683400 reads in 162.289 CPU sec, 5.179 real sec
[M::mem_process_seqs] Processed 682656 reads in 101.150 CPU sec, 3.236 real sec
sorting block 3, #reads = 1080252 mem = 536882017 peak = 856084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282111, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 252, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (274.00, 77.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (337.75, 158.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 285, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1342)
[M::mem_pestat] mean and std.dev: (400.28, 280.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1713)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682166 sequences (100000130 bp) in 2.292 CPU sec, 2.486 real sec...
[M::process] read 681758 sequences (100000277 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281839, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 241, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (343.06, 208.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (337.26, 159.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 231, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (206.42, 89.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682068 reads in 163.314 CPU sec, 5.217 real sec
[M::mem_process_seqs] Processed 682050 reads in 103.524 CPU sec, 3.325 real sec
sorting block 4, #reads = 1078377 mem = 536879996 peak = 856084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282079, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 293, 577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295)
[M::mem_pestat] mean and std.dev: (366.76, 215.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 322, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (335.20, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 277, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (224.46, 80.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685952 sequences (100000069 bp) in 2.154 CPU sec, 2.364 real sec...
[M::process] read 686174 sequences (100000013 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282008, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 338, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (288.75, 139.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (343.48, 163.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (326, 443, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (406.22, 96.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682166 reads in 162.355 CPU sec, 5.205 real sec
sorting block 5, #reads = 1077007 mem = 536879910 peak = 856084480
[M::mem_process_seqs] Processed 681758 reads in 103.712 CPU sec, 3.327 real sec
sorting block 6, #reads = 1076296 mem = 536878372 peak = 856084480
[M::process] read 685188 sequences (100000064 bp) in 2.594 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283742, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 324, 994)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2626)
[M::mem_pestat] mean and std.dev: (492.96, 485.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3442)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 304, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (323.24, 159.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (263.89, 109.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684302 sequences (100000281 bp) in 1.470 CPU sec, 1.469 real sec...
[M::mem_process_seqs] Processed 686174 reads in 88.560 CPU sec, 2.816 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283657, 8, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 199, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (312.12, 244.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1405)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 304, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (324.29, 161.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (288, 373, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 681)
[M::mem_pestat] mean and std.dev: (338.56, 130.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283021, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 338, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (288.81, 106.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 310, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (326.58, 159.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 265, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (225.36, 94.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683770 sequences (100000145 bp) in 1.525 CPU sec, 1.533 real sec...
[M::mem_process_seqs] Processed 685952 reads in 249.137 CPU sec, 7.989 real sec
[M::mem_process_seqs] Processed 685188 reads in 118.198 CPU sec, 3.841 real sec
sorting block 7, #reads = 1081968 mem = 536879797 peak = 856084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282815, 12, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 339, 1076)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2824)
[M::mem_pestat] mean and std.dev: (541.41, 458.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3698)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 316, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (332.23, 161.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (97, 250, 678)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1840)
[M::mem_pestat] mean and std.dev: (275.00, 285.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2421)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 153, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (158.41, 70.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 571)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683250 sequences (100000085 bp) in 2.994 CPU sec, 3.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283074, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (309, 551, 956)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2250)
[M::mem_pestat] mean and std.dev: (572.46, 381.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2897)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 318, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (332.33, 159.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 445, 950)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2348)
[M::mem_pestat] mean and std.dev: (481.18, 304.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3047)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684302 reads in 160.109 CPU sec, 5.157 real sec
sorting block 8, #reads = 1083002 mem = 536882679 peak = 856084480
[M::mem_process_seqs] Processed 683770 reads in 103.737 CPU sec, 3.336 real sec
[M::process] read 683296 sequences (100000294 bp) in 2.013 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282172, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 342, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (296.67, 196.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (336.00, 161.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 9, #reads = 1081288 mem = 536877311 peak = 857440256
[M::process] read 682776 sequences (100000229 bp) in 2.698 CPU sec, 2.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282731, 2, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 412, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381)
[M::mem_pestat] mean and std.dev: (382.17, 163.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1777)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (336.97, 162.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 409, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204)
[M::mem_pestat] mean and std.dev: (411.59, 335.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1755)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683250 reads in 165.942 CPU sec, 5.339 real sec
[M::mem_process_seqs] Processed 683296 reads in 106.805 CPU sec, 3.428 real sec
[M::process] read 682620 sequences (100000047 bp) in 2.264 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282709, 2, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 252, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580)
[M::mem_pestat] mean and std.dev: (245.04, 77.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (337.41, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (349, 563, 1572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4018)
[M::mem_pestat] mean and std.dev: (1014.88, 1088.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5371)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 10, #reads = 1079682 mem = 536882781 peak = 857440256
[M::process] read 682544 sequences (100000277 bp) in 2.396 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282657, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 293, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (310.11, 210.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1356)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (337.61, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 316, 650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1522)
[M::mem_pestat] mean and std.dev: (409.35, 322.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1958)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682776 reads in 164.223 CPU sec, 5.291 real sec
[M::mem_process_seqs] Processed 682620 reads in 105.643 CPU sec, 3.372 real sec
[M::process] read 681922 sequences (100000098 bp) in 2.558 CPU sec, 2.768 real sec...
sorting block 11, #reads = 1079015 mem = 536880565 peak = 857440256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282579, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 242, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (301.06, 172.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (335.83, 160.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 336, 2028)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5612)
[M::mem_pestat] mean and std.dev: (719.75, 806.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7404)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 281962, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 406, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1775)
[M::mem_pestat] mean and std.dev: (415.75, 242.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2302)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (340.26, 162.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 223, 646)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1572)
[M::mem_pestat] mean and std.dev: (373.69, 299.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2035)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682544 reads in 164.467 CPU sec, 5.318 real sec
[M::process] read 684042 sequences (100000057 bp) in 3.342 CPU sec, 3.680 real sec...
[M::mem_process_seqs] Processed 681922 reads in 105.314 CPU sec, 3.396 real sec
sorting block 12, #reads = 1077904 mem = 536882206 peak = 857440256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283592, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (387, 547, 672)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242)
[M::mem_pestat] mean and std.dev: (510.00, 219.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1527)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (334.05, 163.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 363, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (361.80, 227.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687004 sequences (100000019 bp) in 2.555 CPU sec, 2.808 real sec...
[M::mem_process_seqs] Processed 684042 reads in 90.445 CPU sec, 3.019 real sec
sorting block 13, #reads = 1077534 mem = 536880885 peak = 857440256
[M::process] read 684964 sequences (100000089 bp) in 2.406 CPU sec, 2.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284249, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (289.35, 117.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 298, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (318.73, 158.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 312, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (289.45, 106.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684322 sequences (100000175 bp) in 2.108 CPU sec, 2.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283636, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 342, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070)
[M::mem_pestat] mean and std.dev: (263.33, 136.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1378)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 312, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (328.39, 160.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (356, 417, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 863)
[M::mem_pestat] mean and std.dev: (398.39, 168.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687004 reads in 161.747 CPU sec, 5.193 real sec
[M::mem_process_seqs] Processed 684964 reads in 104.165 CPU sec, 3.329 real sec
sorting block 14, #reads = 1080565 mem = 536877790 peak = 857440256
[M::process] read 683588 sequences (100000027 bp) in 2.665 CPU sec, 2.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283195, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 294, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437)
[M::mem_pestat] mean and std.dev: (348.00, 234.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1861)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (335.05, 163.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 389, 995)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2515)
[M::mem_pestat] mean and std.dev: (545.47, 416.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3275)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282495, 0, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 267, 658)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1590)
[M::mem_pestat] mean and std.dev: (419.40, 359.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2056)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (339.31, 165.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 235, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (312.20, 168.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684322 reads in 157.275 CPU sec, 5.105 real sec
[M::mem_process_seqs] Processed 683588 reads in 101.769 CPU sec, 3.306 real sec
[M::process] read 683528 sequences (100000262 bp) in 3.722 CPU sec, 4.065 real sec...
sorting block 15, #reads = 1080562 mem = 536878823 peak = 857440256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283595, 6, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (280, 411, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (414.58, 211.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (339.17, 166.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 301, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (338.14, 196.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683528 reads in 88.032 CPU sec, 2.961 real sec
[M::process] read 682792 sequences (100000197 bp) in 3.174 CPU sec, 3.497 real sec...
sorting block 16, #reads = 1081049 mem = 536877797 peak = 857440256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282550, 11, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 498, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1457)
[M::mem_pestat] mean and std.dev: (443.88, 249.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1881)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (341.24, 165.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 182, 857)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2207)
[M::mem_pestat] mean and std.dev: (445.00, 494.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2882)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 274, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (253.79, 139.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682792 reads in 89.028 CPU sec, 2.998 real sec
[M::process] read 682094 sequences (100000191 bp) in 3.165 CPU sec, 3.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282439, 9, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 232, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (233.42, 98.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (342.93, 165.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 347, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (318.29, 123.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682418 sequences (100000170 bp) in 2.680 CPU sec, 2.945 real sec...
[M::mem_process_seqs] Processed 682094 reads in 88.319 CPU sec, 2.949 real sec
sorting block 17, #reads = 1080241 mem = 536878730 peak = 857440256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282389, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 264, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (279.81, 131.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1358)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (340.10, 164.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 356, 718)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1716)
[M::mem_pestat] mean and std.dev: (433.31, 240.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2215)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682418 reads in 87.440 CPU sec, 2.940 real sec
[M::process] read 682976 sequences (100000045 bp) in 3.111 CPU sec, 3.427 real sec...
sorting block 18, #reads = 1079289 mem = 536877414 peak = 857440256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282664, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 301, 713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1731)
[M::mem_pestat] mean and std.dev: (359.81, 219.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2240)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (339.39, 165.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 364, 992)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2446)
[M::mem_pestat] mean and std.dev: (384.81, 219.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3173)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681854 sequences (100000036 bp) in 2.471 CPU sec, 2.703 real sec...
[M::mem_process_seqs] Processed 682976 reads in 89.004 CPU sec, 2.955 real sec
sorting block 19, #reads = 1078429 mem = 536879007 peak = 857440256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282324, 8, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 227, 1230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420)
[M::mem_pestat] mean and std.dev: (455.33, 470.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682018 sequences (100000148 bp) in 2.410 CPU sec, 2.667 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (342.54, 164.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 329, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432)
[M::mem_pestat] mean and std.dev: (497.96, 411.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2143)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687684 sequences (100000081 bp) in 1.887 CPU sec, 1.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282314, 16, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 280, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (333.82, 174.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (344.52, 167.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 908, 908)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2492)
[M::mem_pestat] mean and std.dev: (771.56, 651.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3376)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 320, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (292.75, 114.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681854 reads in 161.649 CPU sec, 5.209 real sec
[M::mem_process_seqs] Processed 682018 reads in 103.762 CPU sec, 3.317 real sec
[M::process] read 684504 sequences (100000172 bp) in 1.988 CPU sec, 2.128 real sec...
sorting block 20, #reads = 1076789 mem = 536875952 peak = 857440256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283965, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 145, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (222.93, 120.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 294, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (317.62, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 371, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (314.54, 113.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684076 sequences (100000034 bp) in 2.506 CPU sec, 2.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283200, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 210, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (267.75, 172.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1369)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (332.87, 165.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 331, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (381.39, 224.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687684 reads in 157.072 CPU sec, 5.043 real sec
[M::mem_process_seqs] Processed 684504 reads in 100.399 CPU sec, 3.213 real sec
[M::process] read 683244 sequences (100000233 bp) in 2.058 CPU sec, 2.188 real sec...
sorting block 21, #reads = 1077917 mem = 536881980 peak = 857440256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283407, 6, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (262, 430, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (348.76, 143.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (337.95, 166.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 303, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (265.64, 110.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682878 sequences (100000128 bp) in 2.398 CPU sec, 2.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283038, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 182, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (218.22, 103.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (343.03, 168.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 372, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (322.89, 120.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684076 reads in 156.779 CPU sec, 5.024 real sec
[M::process] read 682832 sequences (100000214 bp) in 1.577 CPU sec, 1.607 real sec...
sorting block 22, #reads = 1082343 mem = 536880051 peak = 875610112
[M::mem_process_seqs] Processed 683244 reads in 100.381 CPU sec, 3.203 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282956, 3, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (291, 374, 807)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839)
[M::mem_pestat] mean and std.dev: (439.25, 320.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2355)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (343.19, 167.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 229, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458)
[M::mem_pestat] mean and std.dev: (255.25, 122.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1885)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 23, #reads = 1080501 mem = 536878645 peak = 922218496
[M::process] read 682104 sequences (100000281 bp) in 2.422 CPU sec, 2.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282935, 6, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 335, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (255.82, 96.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (342.21, 166.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 249, 660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1658)
[M::mem_pestat] mean and std.dev: (414.19, 300.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2157)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682878 reads in 158.285 CPU sec, 5.070 real sec
[M::process] read 682092 sequences (100000045 bp) in 1.596 CPU sec, 1.600 real sec...
[M::mem_process_seqs] Processed 682832 reads in 102.359 CPU sec, 3.276 real sec
sorting block 24, #reads = 1079924 mem = 536878662 peak = 922218496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282388, 11, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 188, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (245.46, 141.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (341.16, 163.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (656, 1211, 2310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5618)
[M::mem_pestat] mean and std.dev: (1530.82, 1292.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7272)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 313, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (299.50, 91.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682046 sequences (100000214 bp) in 2.524 CPU sec, 2.672 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282830, 11, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 428, 799)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2041)
[M::mem_pestat] mean and std.dev: (451.93, 282.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2662)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 322, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (337.00, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (452, 706, 1151)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2549)
[M::mem_pestat] mean and std.dev: (737.27, 418.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3248)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 293, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (293.29, 72.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681746 sequences (100000055 bp) in 1.520 CPU sec, 1.523 real sec...
[M::mem_process_seqs] Processed 682104 reads in 158.764 CPU sec, 5.074 real sec
[M::mem_process_seqs] Processed 682092 reads in 101.466 CPU sec, 3.248 real sec
sorting block 25, #reads = 1079219 mem = 536879275 peak = 922218496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282872, 7, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 328, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (295.71, 111.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (338.84, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 329, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353)
[M::mem_pestat] mean and std.dev: (366.33, 197.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1727)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681482 sequences (100000060 bp) in 2.371 CPU sec, 2.603 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282466, 7, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (305, 334, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (398.12, 156.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (341.15, 163.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 372, 657)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561)
[M::mem_pestat] mean and std.dev: (393.35, 232.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2013)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682046 reads in 159.659 CPU sec, 5.134 real sec
[M::mem_process_seqs] Processed 681746 reads in 102.334 CPU sec, 3.267 real sec
[M::process] read 688192 sequences (100000215 bp) in 2.151 CPU sec, 2.256 real sec...
sorting block 26, #reads = 1078380 mem = 536881432 peak = 922218496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281683, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (238.45, 117.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (345.92, 166.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (316, 501, 723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1537)
[M::mem_pestat] mean and std.dev: (537.41, 365.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1997)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1079046 mem = 536879629 peak = 922218496
[M::process] read 689164 sequences (100000137 bp) in 2.601 CPU sec, 2.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284837, 10, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 245, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418)
[M::mem_pestat] mean and std.dev: (397.35, 253.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1816)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 293, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (317.81, 161.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (52, 69, 1017)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2947)
[M::mem_pestat] mean and std.dev: (430.80, 536.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3912)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 318, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (278.43, 137.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681482 reads in 164.717 CPU sec, 5.284 real sec
[M::mem_process_seqs] Processed 688192 reads in 109.994 CPU sec, 3.502 real sec
[M::process] read 687212 sequences (100000147 bp) in 1.851 CPU sec, 1.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285405, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (384, 515, 623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101)
[M::mem_pestat] mean and std.dev: (457.42, 218.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 289, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (313.54, 158.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 274, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (252.18, 118.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 28, #reads = 1076785 mem = 536879395 peak = 922218496
[M::process] read 685884 sequences (100000007 bp) in 2.434 CPU sec, 2.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284495, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 336, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1568)
[M::mem_pestat] mean and std.dev: (463.00, 367.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2037)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 301, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (320.91, 159.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 420, 741)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1879)
[M::mem_pestat] mean and std.dev: (469.46, 335.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2448)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685640 sequences (100000070 bp) in 1.660 CPU sec, 1.652 real sec...
[M::mem_process_seqs] Processed 689164 reads in 165.761 CPU sec, 5.311 real sec
[M::mem_process_seqs] Processed 687212 reads in 104.396 CPU sec, 3.340 real sec
sorting block 29, #reads = 1080605 mem = 536877208 peak = 922218496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284276, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 337, 1629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4489)
[M::mem_pestat] mean and std.dev: (781.00, 666.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5919)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 309, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (327.46, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 340, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (284.31, 75.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685070 sequences (100000046 bp) in 2.866 CPU sec, 3.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283667, 9, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 210, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (171.50, 46.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 311, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (328.01, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684828 sequences (100000194 bp) in 1.640 CPU sec, 1.643 real sec...
[M::mem_process_seqs] Processed 685884 reads in 165.046 CPU sec, 5.266 real sec
[M::mem_process_seqs] Processed 685640 reads in 105.314 CPU sec, 3.363 real sec
sorting block 30, #reads = 1080337 mem = 536878435 peak = 922218496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283785, 8, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 287, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (244.92, 70.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 716)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 314, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (329.80, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 384, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (361.58, 126.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684648 sequences (100000225 bp) in 3.201 CPU sec, 3.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283598, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (266, 380, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (329.22, 123.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (331.01, 161.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 409, 787)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2007)
[M::mem_pestat] mean and std.dev: (555.28, 502.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2617)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685070 reads in 168.656 CPU sec, 5.440 real sec
[M::mem_process_seqs] Processed 684828 reads in 108.287 CPU sec, 3.477 real sec
sorting block 31, #reads = 1079509 mem = 536883219 peak = 922218496
[M::process] read 683606 sequences (100000285 bp) in 2.888 CPU sec, 3.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283370, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 335, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (286.07, 135.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (331.49, 160.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 295, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (275.05, 157.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 32, #reads = 1077628 mem = 536880666 peak = 922218496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283080, 7, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 262, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 583)
[M::mem_pestat] mean and std.dev: (240.94, 64.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 692)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 318, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (332.48, 158.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 401, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (361.21, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682940 sequences (100000244 bp) in 2.720 CPU sec, 2.975 real sec...
[M::mem_process_seqs] Processed 684648 reads in 172.461 CPU sec, 5.581 real sec
[M::mem_process_seqs] Processed 683606 reads in 109.320 CPU sec, 3.505 real sec
[M::process] read 683656 sequences (100000154 bp) in 2.482 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283223, 7, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 200, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (202.14, 68.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (337.19, 159.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 659)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561)
[M::mem_pestat] mean and std.dev: (433.33, 371.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2012)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 33, #reads = 1077641 mem = 536878135 peak = 922218496
[M::process] read 695700 sequences (100000252 bp) in 2.306 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283447, 0, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 295, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2018)
[M::mem_pestat] mean and std.dev: (401.00, 292.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2632)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 320, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (335.95, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 400, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (341.38, 112.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682940 reads in 172.355 CPU sec, 5.529 real sec
[M::mem_process_seqs] Processed 683656 reads in 111.278 CPU sec, 3.546 real sec
[M::process] read 691842 sequences (100000223 bp) in 2.278 CPU sec, 2.453 real sec...
sorting block 34, #reads = 1074871 mem = 536880449 peak = 922218496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 288222, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (286, 381, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (320.12, 134.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 253, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (281.92, 143.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 278, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (101, 396)
[M::mem_pestat] mean and std.dev: (229.64, 52.07)
[M::mem_pestat] low and high boundaries for proper pairs: (21, 455)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688924 sequences (100000143 bp) in 2.524 CPU sec, 2.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286431, 3, 21)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 274, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (297.97, 148.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 265, 1019)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609)
[M::mem_pestat] mean and std.dev: (350.61, 267.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3404)
[M::mem_pestat] skip orientation RR
[M::process] read 687404 sequences (100000122 bp) in 1.530 CPU sec, 1.523 real sec...
[M::mem_process_seqs] Processed 695700 reads in 186.923 CPU sec, 5.976 real sec
[M::mem_process_seqs] Processed 691842 reads in 117.716 CPU sec, 3.767 real sec
sorting block 35, #reads = 1073627 mem = 536877417 peak = 922218496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 285767, 8, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 382, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546)
[M::mem_pestat] mean and std.dev: (504.12, 429.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2221)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 288, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (308.44, 151.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 252, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (352.81, 258.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1387)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686552 sequences (100000049 bp) in 2.532 CPU sec, 2.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285411, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 246, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (225.32, 87.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 298, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (315.77, 154.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 208, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (246.12, 128.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685734 sequences (100000249 bp) in 1.640 CPU sec, 1.647 real sec...
[M::mem_process_seqs] Processed 688924 reads in 177.355 CPU sec, 5.695 real sec
sorting block 36, #reads = 1074692 mem = 536876577 peak = 922218496
[M::mem_process_seqs] Processed 687404 reads in 111.285 CPU sec, 3.564 real sec
sorting block 37, #reads = 1074859 mem = 536879062 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285063, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 174, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (212.79, 83.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 302, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (319.95, 156.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 357, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (272.07, 130.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684526 sequences (100000245 bp) in 2.647 CPU sec, 2.826 real sec...
[M::process] read 683450 sequences (100000180 bp) in 1.630 CPU sec, 1.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284023, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 219, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (223.25, 68.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 557)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (328.18, 161.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 287, 671)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531)
[M::mem_pestat] mean and std.dev: (337.85, 181.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1961)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686552 reads in 171.236 CPU sec, 5.465 real sec
[M::mem_process_seqs] Processed 685734 reads in 107.216 CPU sec, 3.424 real sec
sorting block 38, #reads = 1075966 mem = 536878413 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283466, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 269, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290)
[M::mem_pestat] mean and std.dev: (384.07, 255.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1641)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 318, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (335.24, 165.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 371, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317)
[M::mem_pestat] mean and std.dev: (432.87, 254.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1669)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682688 sequences (100000043 bp) in 2.479 CPU sec, 2.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282958, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (281.48, 147.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (339.03, 166.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 222, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (246.37, 108.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687662 sequences (100000218 bp) in 1.680 CPU sec, 1.674 real sec...
[M::mem_process_seqs] Processed 684526 reads in 170.254 CPU sec, 5.442 real sec
[M::mem_process_seqs] Processed 683450 reads in 107.793 CPU sec, 3.440 real sec
sorting block 39, #reads = 1076582 mem = 536879143 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282568, 3, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 264, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (319.25, 207.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 327, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (345.30, 170.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 307, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482)
[M::mem_pestat] mean and std.dev: (333.73, 184.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1912)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687772 sequences (100000053 bp) in 2.878 CPU sec, 3.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284258, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 227, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (282.44, 152.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 295, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (319.66, 162.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 320, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1383)
[M::mem_pestat] mean and std.dev: (365.12, 238.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1782)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686476 sequences (100000008 bp) in 1.660 CPU sec, 1.657 real sec...
[M::mem_process_seqs] Processed 682688 reads in 176.006 CPU sec, 5.616 real sec
sorting block 40, #reads = 1074878 mem = 536879980 peak = 1006088192
[M::mem_process_seqs] Processed 687662 reads in 114.855 CPU sec, 3.666 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285012, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 227, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (251.52, 110.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 294, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (316.29, 158.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 211, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 503)
[M::mem_pestat] mean and std.dev: (208.82, 50.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 41, #reads = 1072106 mem = 536880768 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284615, 5, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 175, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (221.94, 76.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 303, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (323.16, 160.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 270, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (270.46, 117.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685404 sequences (100000072 bp) in 3.299 CPU sec, 3.613 real sec...
[M::mem_process_seqs] Processed 687772 reads in 161.144 CPU sec, 5.207 real sec
[M::mem_process_seqs] Processed 686476 reads in 103.654 CPU sec, 3.331 real sec
[M::process] read 684664 sequences (100000255 bp) in 2.463 CPU sec, 2.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284197, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 252, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (252.19, 103.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 309, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (326.44, 160.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 236, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (270.26, 131.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 42, #reads = 1080874 mem = 536877556 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283480, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 206, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (308.92, 251.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (331.09, 162.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 266, 850)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2046)
[M::mem_pestat] mean and std.dev: (434.00, 266.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2644)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685404 reads in 161.238 CPU sec, 5.199 real sec
[M::mem_process_seqs] Processed 684664 reads in 103.964 CPU sec, 3.356 real sec
[M::process] read 685524 sequences (100000098 bp) in 3.272 CPU sec, 3.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284071, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (281, 351, 1959)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5315)
[M::mem_pestat] mean and std.dev: (701.76, 710.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6993)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 308, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (326.77, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 285, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (278.21, 119.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685524 reads in 89.491 CPU sec, 3.007 real sec
sorting block 43, #reads = 1079927 mem = 536881285 peak = 1006088192
[M::process] read 684350 sequences (100000120 bp) in 3.490 CPU sec, 3.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283789, 5, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (326, 384, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (339.13, 123.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (334.08, 164.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 259, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (274.93, 86.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683668 sequences (100000194 bp) in 2.581 CPU sec, 2.835 real sec...
[M::mem_process_seqs] Processed 684350 reads in 91.462 CPU sec, 3.067 real sec
sorting block 44, #reads = 1079249 mem = 536880509 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283165, 7, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 250, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (324.56, 245.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (338.82, 166.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 268, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (291.08, 106.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683592 sequences (100000225 bp) in 2.622 CPU sec, 2.872 real sec...
[M::mem_process_seqs] Processed 683668 reads in 91.972 CPU sec, 3.057 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283175, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 452, 1233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3399)
[M::mem_pestat] mean and std.dev: (461.47, 362.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4482)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (338.17, 166.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 249, 996)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2584)
[M::mem_pestat] mean and std.dev: (360.11, 340.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3378)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683592 reads in 93.521 CPU sec, 3.128 real sec
sorting block 45, #reads = 1078123 mem = 536882218 peak = 1006088192
[M::process] read 683154 sequences (100000071 bp) in 3.415 CPU sec, 3.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283081, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 347, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1314)
[M::mem_pestat] mean and std.dev: (308.00, 145.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1689)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (339.83, 166.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 337, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (332.31, 154.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683154 reads in 94.145 CPU sec, 3.167 real sec
[M::process] read 683130 sequences (100000005 bp) in 3.458 CPU sec, 3.797 real sec...
sorting block 46, #reads = 1075666 mem = 536882669 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283375, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 235, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156)
[M::mem_pestat] mean and std.dev: (315.90, 218.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (342.33, 168.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 266, 946)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2462)
[M::mem_pestat] mean and std.dev: (456.31, 425.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3220)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683130 reads in 97.590 CPU sec, 3.261 real sec
[M::process] read 694132 sequences (100000217 bp) in 3.534 CPU sec, 3.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286893, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 248, 583)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345)
[M::mem_pestat] mean and std.dev: (422.55, 323.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1726)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (290.95, 149.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 253, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (296.41, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 777)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 47, #reads = 1074398 mem = 536878390 peak = 1006088192
[M::mem_process_seqs] Processed 694132 reads in 104.650 CPU sec, 3.467 real sec
[M::process] read 690042 sequences (100000202 bp) in 3.427 CPU sec, 3.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286195, 7, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (342, 417, 1492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3792)
[M::mem_pestat] mean and std.dev: (865.56, 942.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4942)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 284, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (306.97, 153.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 390, 706)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614)
[M::mem_pestat] mean and std.dev: (377.38, 187.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2068)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690042 reads in 99.616 CPU sec, 3.296 real sec
[M::process] read 687432 sequences (100000215 bp) in 3.482 CPU sec, 3.798 real sec...
sorting block 48, #reads = 1070962 mem = 536885486 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 285047, 8, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 385, 566)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292)
[M::mem_pestat] mean and std.dev: (329.32, 192.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1655)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 301, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (319.40, 157.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 278, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (283.85, 93.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685856 sequences (100000047 bp) in 2.944 CPU sec, 3.219 real sec...
[M::mem_process_seqs] Processed 687432 reads in 97.374 CPU sec, 3.219 real sec
sorting block 49, #reads = 1073907 mem = 536879971 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284511, 9, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 193, 568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1410)
[M::mem_pestat] mean and std.dev: (274.95, 183.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1831)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (326.24, 159.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 238, 975)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2563)
[M::mem_pestat] mean and std.dev: (395.50, 318.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3357)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684760 sequences (100000268 bp) in 2.650 CPU sec, 2.956 real sec...
[M::mem_process_seqs] Processed 685856 reads in 97.763 CPU sec, 3.221 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284091, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 276, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (346.91, 267.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1415)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (331.54, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473)
[M::mem_pestat] mean and std.dev: (330.46, 227.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1909)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684760 reads in 96.476 CPU sec, 3.142 real sec
[M::process] read 684024 sequences (100000074 bp) in 3.359 CPU sec, 3.725 real sec...
sorting block 50, #reads = 1073572 mem = 536881252 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283747, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 359, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340)
[M::mem_pestat] mean and std.dev: (413.00, 264.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1711)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (333.40, 161.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 405, 853)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2055)
[M::mem_pestat] mean and std.dev: (598.30, 503.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2656)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683088 sequences (100000282 bp) in 2.743 CPU sec, 3.025 real sec...
[M::mem_process_seqs] Processed 684024 reads in 95.183 CPU sec, 3.123 real sec
sorting block 51, #reads = 1074475 mem = 536880774 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283193, 8, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 347, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129)
[M::mem_pestat] mean and std.dev: (347.05, 193.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1433)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (336.70, 163.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (317.95, 175.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683088 reads in 93.188 CPU sec, 3.037 real sec
[M::process] read 683062 sequences (100000136 bp) in 3.317 CPU sec, 3.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282635, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 220, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (229.20, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (338.10, 163.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 236, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265)
[M::mem_pestat] mean and std.dev: (279.55, 185.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1618)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682352 sequences (100000021 bp) in 2.579 CPU sec, 2.837 real sec...
[M::mem_process_seqs] Processed 683062 reads in 93.079 CPU sec, 3.040 real sec
sorting block 52, #reads = 1075576 mem = 536877655 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282769, 1, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 240, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (273.35, 127.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (343.33, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 338, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (91, 556)
[M::mem_pestat] mean and std.dev: (301.50, 77.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 646478 sequences (94746312 bp) in 2.532 CPU sec, 2.832 real sec...
[M::mem_process_seqs] Processed 682352 reads in 91.901 CPU sec, 2.992 real sec
sorting block 53, #reads = 1075255 mem = 536878223 peak = 1006088192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 268102, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 270, 750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1858)
[M::mem_pestat] mean and std.dev: (357.45, 248.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2412)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (345.65, 169.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 309, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (295.20, 97.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1373)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 646478 reads in 84.743 CPU sec, 2.805 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 58844382
overall: 69744160768 mem 9377.402 user 650.424 sys 771.357 real
sorting block 54, #reads = 945113 mem = 471598933 peak = 1006088192
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 771.414 sec; CPU: 10027.850 sec
uniq header count 1
merging 55 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3601943436
output reads: 59158247
bam_mem_sort: 55 calls 22.428 user 0.507 sys 22.983 real
bam_write: 55 calls 45.204 user 1.209 sys 46.686 real
execute: 1 calls 12.505 user 1.084 sys 793.500 real
merge_files: 1 calls 12.494 user 1.044 sys 20.813 real
parse_chunk: 879 calls 28.636 user 7.788 sys 37.380 real
read_chunk: 2372 calls 30.751 user 26.089 sys 271.595 real
sort_block: 1 calls 25.692 user 25.394 sys 263.500 real
write_chunk: 1491 calls 12.169 user 0.779 sys 19.980 real
overall: 2548674560 mem 781.234 user 43.263 sys 793.522 real
ls: cannot access '*.cram': No such file or directory