.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_cVw-T1-TDNA-1_A23MHWWLT4_1;L006;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/02/a7b05caaab156822500189bd42c988/.command.err
Size: 227.0 KB
Task: DAQ:SENTIEON_BWAMEM (659_cVw-T1-TDNA-1_A23MHWWLT4_1;L006;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cVw-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1337.658 CPU sec, 47.782 real sec
[M::process] read 683814 sequences (100000147 bp) in 1.511 CPU sec, 1.512 real sec...
[M::process] read 680946 sequences (100000092 bp) in 1.594 CPU sec, 1.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284968, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 293, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (317.78, 154.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680704 sequences (100000191 bp) in 1.676 CPU sec, 1.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283429, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 312, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (333.08, 158.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680464 sequences (100000166 bp) in 1.679 CPU sec, 1.721 real sec...
[M::mem_process_seqs] Processed 683814 reads in 163.811 CPU sec, 5.435 real sec
[M::mem_process_seqs] Processed 680946 reads in 110.241 CPU sec, 3.805 real sec
sorting block 0, #reads = 1084737 mem = 536880429 peak = 599064576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283260, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (336.10, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680322 sequences (100000158 bp) in 2.113 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283625, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (336.89, 159.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679720 sequences (100000086 bp) in 1.815 CPU sec, 1.914 real sec...
[M::mem_process_seqs] Processed 680704 reads in 161.960 CPU sec, 5.443 real sec
[M::mem_process_seqs] Processed 680464 reads in 109.930 CPU sec, 3.744 real sec
sorting block 1, #reads = 1084222 mem = 536880820 peak = 736690176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283318, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (337.97, 159.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679650 sequences (100000088 bp) in 1.982 CPU sec, 2.332 real sec...
[M::process] read 679336 sequences (100000031 bp) in 1.795 CPU sec, 1.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283255, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (339.71, 159.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680322 reads in 160.847 CPU sec, 5.447 real sec
[M::mem_process_seqs] Processed 679720 reads in 109.433 CPU sec, 3.714 real sec
sorting block 2, #reads = 1084440 mem = 536879897 peak = 857722880
[M::process] read 679082 sequences (100000218 bp) in 2.240 CPU sec, 2.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284108, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (344.47, 161.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678444 sequences (100000191 bp) in 1.841 CPU sec, 1.846 real sec...
[M::mem_process_seqs] Processed 679336 reads in 92.784 CPU sec, 3.045 real sec
sorting block 3, #reads = 1083851 mem = 536880975 peak = 918642688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283717, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 324, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (342.21, 160.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 474, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190)
[M::mem_pestat] mean and std.dev: (344.00, 169.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1548)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282869, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (344.87, 160.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679650 reads in 252.113 CPU sec, 8.472 real sec
[M::process] read 678462 sequences (100000148 bp) in 1.786 CPU sec, 1.973 real sec...
[M::mem_process_seqs] Processed 679082 reads in 128.961 CPU sec, 4.509 real sec
sorting block 4, #reads = 1083541 mem = 536879374 peak = 918642688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283005, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (348.37, 162.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678380 sequences (100000105 bp) in 2.151 CPU sec, 2.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283380, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.20, 164.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678258 sequences (100000263 bp) in 1.872 CPU sec, 1.886 real sec...
[M::mem_process_seqs] Processed 678444 reads in 161.143 CPU sec, 5.495 real sec
[M::mem_process_seqs] Processed 678462 reads in 110.006 CPU sec, 3.841 real sec
sorting block 5, #reads = 1082472 mem = 536878651 peak = 918642688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282601, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (349.99, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678454 sequences (100000136 bp) in 2.250 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282731, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (349.34, 161.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677994 sequences (100000133 bp) in 1.821 CPU sec, 1.897 real sec...
[M::mem_process_seqs] Processed 678380 reads in 159.769 CPU sec, 5.508 real sec
sorting block 6, #reads = 1082693 mem = 536878748 peak = 945176576
[M::mem_process_seqs] Processed 678258 reads in 108.196 CPU sec, 3.839 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282897, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (353.26, 164.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1082246 mem = 536879545 peak = 998674432
[M::process] read 677700 sequences (100000214 bp) in 2.198 CPU sec, 2.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282917, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (354.23, 164.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678454 reads in 160.310 CPU sec, 5.591 real sec
[M::process] read 677550 sequences (100000008 bp) in 1.950 CPU sec, 1.983 real sec...
[M::mem_process_seqs] Processed 677994 reads in 109.427 CPU sec, 3.879 real sec
sorting block 8, #reads = 1083277 mem = 536879725 peak = 998674432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282518, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (354.13, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677760 sequences (100000003 bp) in 2.396 CPU sec, 2.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282563, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (357.41, 165.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677700 reads in 161.466 CPU sec, 5.425 real sec
[M::process] read 677558 sequences (100000185 bp) in 1.964 CPU sec, 2.033 real sec...
[M::mem_process_seqs] Processed 677550 reads in 110.526 CPU sec, 3.717 real sec
sorting block 9, #reads = 1082359 mem = 536876147 peak = 998674432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282414, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (353.59, 163.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677578 sequences (100000019 bp) in 2.234 CPU sec, 2.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282550, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (355.26, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677760 reads in 161.207 CPU sec, 5.553 real sec
[M::process] read 677574 sequences (100000271 bp) in 2.013 CPU sec, 2.190 real sec...
[M::mem_process_seqs] Processed 677558 reads in 110.892 CPU sec, 3.827 real sec
sorting block 10, #reads = 1082524 mem = 536879378 peak = 998674432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282484, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (354.08, 163.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677362 sequences (100000042 bp) in 2.464 CPU sec, 3.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282635, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (354.35, 164.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677578 reads in 163.773 CPU sec, 5.612 real sec
sorting block 11, #reads = 1082331 mem = 536878432 peak = 1018089472
[M::mem_process_seqs] Processed 677574 reads in 112.243 CPU sec, 3.831 real sec
[M::process] read 677414 sequences (100000213 bp) in 2.132 CPU sec, 2.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282604, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (350.99, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 12, #reads = 1081635 mem = 536881923 peak = 1056112640
[M::process] read 677286 sequences (100000061 bp) in 2.182 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282390, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (350.58, 160.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677362 reads in 163.451 CPU sec, 5.647 real sec
[M::mem_process_seqs] Processed 677414 reads in 112.860 CPU sec, 3.873 real sec
[M::process] read 677408 sequences (100000269 bp) in 2.177 CPU sec, 2.469 real sec...
sorting block 13, #reads = 1081306 mem = 536881427 peak = 1056112640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282538, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (349.37, 159.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677610 sequences (100000093 bp) in 2.400 CPU sec, 2.767 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282111, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (346.05, 158.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677286 reads in 164.363 CPU sec, 5.596 real sec
[M::process] read 677740 sequences (100000110 bp) in 2.037 CPU sec, 2.239 real sec...
[M::mem_process_seqs] Processed 677408 reads in 111.780 CPU sec, 3.948 real sec
sorting block 14, #reads = 1081935 mem = 536878544 peak = 1056112640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282221, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (344.80, 157.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677884 sequences (100000231 bp) in 2.213 CPU sec, 2.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282171, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (345.06, 157.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677610 reads in 163.599 CPU sec, 5.579 real sec
[M::mem_process_seqs] Processed 677740 reads in 111.770 CPU sec, 3.808 real sec
sorting block 15, #reads = 1081168 mem = 536879708 peak = 1056112640
[M::process] read 677876 sequences (100000143 bp) in 2.126 CPU sec, 2.470 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282697, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (342.37, 156.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677656 sequences (100000065 bp) in 2.438 CPU sec, 3.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282290, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 328, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (344.41, 157.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677884 reads in 164.419 CPU sec, 5.588 real sec
sorting block 16, #reads = 1081616 mem = 536878892 peak = 1062547456
[M::mem_process_seqs] Processed 677876 reads in 111.620 CPU sec, 3.872 real sec
[M::process] read 677630 sequences (100000010 bp) in 2.192 CPU sec, 2.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282674, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (343.22, 156.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1081645 mem = 536876992 peak = 1087266816
[M::process] read 677656 sequences (100000232 bp) in 2.315 CPU sec, 2.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282811, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (343.90, 157.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677656 reads in 165.898 CPU sec, 5.652 real sec
[M::process] read 677392 sequences (100000057 bp) in 2.117 CPU sec, 2.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282503, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 328, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (345.16, 157.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677656 reads in 93.605 CPU sec, 3.140 real sec
[M::process] read 677522 sequences (100000200 bp) in 2.302 CPU sec, 2.388 real sec...
sorting block 18, #reads = 1081979 mem = 536880048 peak = 1087266816
[M::mem_process_seqs] Processed 677630 reads in 261.505 CPU sec, 8.949 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282806, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (345.54, 158.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1080984 mem = 536879959 peak = 1095860224
[M::process] read 677324 sequences (100000079 bp) in 2.381 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282583, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (346.59, 158.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677392 reads in 185.477 CPU sec, 6.431 real sec
[M::mem_process_seqs] Processed 677522 reads in 115.330 CPU sec, 3.966 real sec
[M::process] read 676940 sequences (100000211 bp) in 2.399 CPU sec, 2.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282408, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (350.23, 159.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1081661 mem = 536879505 peak = 1095860224
[M::process] read 676960 sequences (100000175 bp) in 2.376 CPU sec, 2.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282456, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.67, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677324 reads in 165.517 CPU sec, 5.608 real sec
[M::mem_process_seqs] Processed 676940 reads in 113.707 CPU sec, 3.834 real sec
[M::process] read 679546 sequences (100000118 bp) in 2.499 CPU sec, 2.947 real sec...
sorting block 21, #reads = 1079031 mem = 536879707 peak = 1095860224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283113, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 336, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (352.04, 160.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680838 sequences (100000099 bp) in 2.161 CPU sec, 2.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283569, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (339.78, 159.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676960 reads in 166.119 CPU sec, 5.623 real sec
[M::mem_process_seqs] Processed 679546 reads in 115.635 CPU sec, 3.938 real sec
[M::process] read 680906 sequences (100000098 bp) in 2.263 CPU sec, 2.459 real sec...
sorting block 22, #reads = 1080065 mem = 536878555 peak = 1095860224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283300, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 315, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (335.61, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680144 sequences (100000107 bp) in 2.477 CPU sec, 2.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283816, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.23, 160.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680838 reads in 160.030 CPU sec, 5.391 real sec
sorting block 23, #reads = 1081588 mem = 536879855 peak = 1095860224
[M::mem_process_seqs] Processed 680906 reads in 109.264 CPU sec, 3.646 real sec
[M::process] read 680172 sequences (100000297 bp) in 2.200 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283401, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (339.03, 160.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1085913 mem = 536879787 peak = 1106808832
[M::process] read 679832 sequences (100000026 bp) in 2.315 CPU sec, 2.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282662, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (339.32, 160.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680144 reads in 159.402 CPU sec, 5.332 real sec
[M::mem_process_seqs] Processed 680172 reads in 108.499 CPU sec, 3.575 real sec
[M::process] read 679636 sequences (100000134 bp) in 2.236 CPU sec, 2.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283299, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (341.25, 160.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1085371 mem = 536884219 peak = 1106808832
[M::process] read 679456 sequences (100000107 bp) in 2.462 CPU sec, 2.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282951, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (342.57, 160.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679832 reads in 159.569 CPU sec, 5.156 real sec
[M::mem_process_seqs] Processed 679636 reads in 107.639 CPU sec, 3.445 real sec
[M::process] read 679260 sequences (100000246 bp) in 2.203 CPU sec, 2.260 real sec...
sorting block 26, #reads = 1085203 mem = 536880523 peak = 1106808832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283037, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (344.39, 161.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679098 sequences (100000005 bp) in 2.356 CPU sec, 2.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283173, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (345.31, 161.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679456 reads in 160.986 CPU sec, 5.204 real sec
[M::mem_process_seqs] Processed 679260 reads in 110.062 CPU sec, 3.503 real sec
[M::process] read 678788 sequences (100000281 bp) in 2.089 CPU sec, 2.151 real sec...
sorting block 27, #reads = 1085123 mem = 536879131 peak = 1106808832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283253, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (347.80, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678300 sequences (100000132 bp) in 2.591 CPU sec, 2.787 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282969, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (349.74, 162.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679098 reads in 158.938 CPU sec, 5.109 real sec
sorting block 28, #reads = 1083733 mem = 536876993 peak = 1106808832
[M::mem_process_seqs] Processed 678788 reads in 108.142 CPU sec, 3.439 real sec
[M::process] read 678254 sequences (100000195 bp) in 2.157 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282971, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (350.97, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1083685 mem = 536880039 peak = 1106808832
[M::process] read 678206 sequences (100000231 bp) in 2.493 CPU sec, 2.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282619, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (351.83, 163.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678300 reads in 156.511 CPU sec, 5.004 real sec
[M::mem_process_seqs] Processed 678254 reads in 105.885 CPU sec, 3.336 real sec
[M::process] read 678134 sequences (100000301 bp) in 2.262 CPU sec, 2.299 real sec...
sorting block 30, #reads = 1083969 mem = 536878011 peak = 1106808832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282676, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (350.52, 162.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678082 sequences (100000001 bp) in 2.523 CPU sec, 2.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282832, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (351.21, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678206 reads in 151.831 CPU sec, 4.813 real sec
[M::process] read 677696 sequences (100000035 bp) in 1.979 CPU sec, 1.999 real sec...
[M::mem_process_seqs] Processed 678134 reads in 101.954 CPU sec, 3.202 real sec
sorting block 31, #reads = 1083074 mem = 536878794 peak = 1106808832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282815, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (353.69, 163.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677654 sequences (100000212 bp) in 2.279 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282328, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (356.23, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677664 sequences (100000284 bp) in 1.678 CPU sec, 1.685 real sec...
[M::mem_process_seqs] Processed 678082 reads in 151.716 CPU sec, 4.807 real sec
sorting block 32, #reads = 1084101 mem = 536882013 peak = 1106808832
[M::mem_process_seqs] Processed 677696 reads in 102.906 CPU sec, 3.248 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282886, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (356.98, 165.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677396 sequences (100000267 bp) in 2.182 CPU sec, 2.243 real sec...
[M::process] read 677200 sequences (100000143 bp) in 1.610 CPU sec, 1.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282799, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 340, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.84, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677654 reads in 150.457 CPU sec, 4.763 real sec
sorting block 33, #reads = 1082780 mem = 536879363 peak = 1106808832
[M::mem_process_seqs] Processed 677664 reads in 99.623 CPU sec, 3.158 real sec
sorting block 34, #reads = 1083026 mem = 536880101 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282569, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 340, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (354.69, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676928 sequences (100000182 bp) in 2.030 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281973, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (358.60, 165.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 479, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353)
[M::mem_pestat] mean and std.dev: (287.60, 206.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1790)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677662 sequences (100000011 bp) in 1.890 CPU sec, 1.893 real sec...
[M::mem_process_seqs] Processed 677396 reads in 151.800 CPU sec, 4.809 real sec
[M::mem_process_seqs] Processed 677200 reads in 101.882 CPU sec, 3.216 real sec
sorting block 35, #reads = 1083026 mem = 536879252 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282244, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (356.98, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677098 sequences (100000082 bp) in 2.602 CPU sec, 2.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282720, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 328, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (344.32, 156.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677154 sequences (100000176 bp) in 2.000 CPU sec, 1.998 real sec...
[M::mem_process_seqs] Processed 676928 reads in 157.809 CPU sec, 4.982 real sec
[M::mem_process_seqs] Processed 677662 reads in 107.781 CPU sec, 3.405 real sec
sorting block 36, #reads = 1081496 mem = 536877695 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282427, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (349.04, 158.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677498 sequences (100000080 bp) in 2.287 CPU sec, 2.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282739, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (348.78, 158.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677348 sequences (100000208 bp) in 1.992 CPU sec, 1.996 real sec...
[M::mem_process_seqs] Processed 677098 reads in 155.979 CPU sec, 4.939 real sec
sorting block 37, #reads = 1081685 mem = 536877457 peak = 1127256064
[M::mem_process_seqs] Processed 677154 reads in 104.952 CPU sec, 3.319 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282113, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.14, 157.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1080913 mem = 536884231 peak = 1127256064
[M::process] read 677576 sequences (100000141 bp) in 2.569 CPU sec, 2.640 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282323, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (346.41, 157.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677796 sequences (100000120 bp) in 1.925 CPU sec, 1.926 real sec...
[M::mem_process_seqs] Processed 677498 reads in 155.862 CPU sec, 4.929 real sec
[M::mem_process_seqs] Processed 677348 reads in 103.997 CPU sec, 3.289 real sec
sorting block 39, #reads = 1081558 mem = 536878461 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282435, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (344.98, 157.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677440 sequences (100000211 bp) in 2.280 CPU sec, 2.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282471, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (346.41, 158.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 33, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (104.33, 122.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677370 sequences (100000103 bp) in 1.965 CPU sec, 1.966 real sec...
[M::mem_process_seqs] Processed 677576 reads in 157.943 CPU sec, 4.997 real sec
[M::mem_process_seqs] Processed 677796 reads in 105.740 CPU sec, 3.340 real sec
sorting block 40, #reads = 1081351 mem = 536882360 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282453, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (347.30, 158.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677716 sequences (100000151 bp) in 2.622 CPU sec, 2.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282047, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (348.67, 159.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677344 sequences (100000128 bp) in 1.980 CPU sec, 1.985 real sec...
[M::mem_process_seqs] Processed 677440 reads in 159.906 CPU sec, 5.061 real sec
[M::mem_process_seqs] Processed 677370 reads in 107.982 CPU sec, 3.404 real sec
sorting block 41, #reads = 1082040 mem = 536879412 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282800, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (345.30, 158.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677676 sequences (100000287 bp) in 2.699 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282144, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (346.64, 158.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677630 sequences (100000288 bp) in 1.966 CPU sec, 1.970 real sec...
[M::mem_process_seqs] Processed 677716 reads in 158.422 CPU sec, 5.012 real sec
sorting block 42, #reads = 1081095 mem = 536876607 peak = 1127256064
[M::mem_process_seqs] Processed 677344 reads in 107.141 CPU sec, 3.383 real sec
sorting block 43, #reads = 1081308 mem = 536879052 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282390, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (346.99, 159.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677408 sequences (100000221 bp) in 2.344 CPU sec, 2.404 real sec...
[M::process] read 677540 sequences (100000001 bp) in 1.650 CPU sec, 1.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282670, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (348.49, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677676 reads in 155.151 CPU sec, 4.917 real sec
[M::mem_process_seqs] Processed 677630 reads in 104.133 CPU sec, 3.295 real sec
sorting block 44, #reads = 1080730 mem = 536877423 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282224, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (351.04, 161.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677402 sequences (100000174 bp) in 2.592 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283282, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (352.48, 162.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679172 sequences (100000168 bp) in 1.940 CPU sec, 1.944 real sec...
[M::mem_process_seqs] Processed 677408 reads in 155.265 CPU sec, 4.896 real sec
[M::mem_process_seqs] Processed 677540 reads in 104.395 CPU sec, 3.292 real sec
sorting block 45, #reads = 1080380 mem = 536878281 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282559, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (353.91, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681490 sequences (100000091 bp) in 2.324 CPU sec, 2.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283685, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (343.47, 161.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681578 sequences (100000266 bp) in 1.950 CPU sec, 1.945 real sec...
[M::mem_process_seqs] Processed 677402 reads in 156.531 CPU sec, 4.945 real sec
sorting block 46, #reads = 1079374 mem = 536880007 peak = 1127256064
[M::mem_process_seqs] Processed 679172 reads in 104.952 CPU sec, 3.320 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283966, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 311, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (331.47, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681402 sequences (100000271 bp) in 2.571 CPU sec, 2.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283596, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 311, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (332.02, 158.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681174 sequences (100000145 bp) in 1.910 CPU sec, 1.907 real sec...
[M::mem_process_seqs] Processed 681490 reads in 155.530 CPU sec, 4.906 real sec
sorting block 47, #reads = 1081268 mem = 536881626 peak = 1127256064
[M::mem_process_seqs] Processed 681578 reads in 102.377 CPU sec, 3.233 real sec
sorting block 48, #reads = 1084846 mem = 536878778 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283574, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 311, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (332.39, 158.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681010 sequences (100000268 bp) in 2.593 CPU sec, 2.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283423, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (333.85, 158.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680686 sequences (100000253 bp) in 1.940 CPU sec, 1.943 real sec...
[M::mem_process_seqs] Processed 681402 reads in 153.489 CPU sec, 4.839 real sec
[M::mem_process_seqs] Processed 681174 reads in 102.247 CPU sec, 3.226 real sec
sorting block 49, #reads = 1085301 mem = 536881002 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284050, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (335.80, 159.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680230 sequences (100000175 bp) in 2.627 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283127, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (337.26, 159.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679886 sequences (100000198 bp) in 1.920 CPU sec, 1.922 real sec...
[M::mem_process_seqs] Processed 681010 reads in 150.354 CPU sec, 4.735 real sec
[M::mem_process_seqs] Processed 680686 reads in 100.660 CPU sec, 3.175 real sec
sorting block 50, #reads = 1085368 mem = 536881579 peak = 1127256064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283560, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (338.19, 159.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679642 sequences (100000254 bp) in 2.426 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283488, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (341.65, 160.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679272 sequences (100000220 bp) in 1.700 CPU sec, 1.692 real sec...
[M::mem_process_seqs] Processed 680230 reads in 146.673 CPU sec, 4.632 real sec
sorting block 51, #reads = 1083009 mem = 536881692 peak = 1127256064
[M::mem_process_seqs] Processed 679886 reads in 96.476 CPU sec, 3.046 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282981, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (343.04, 161.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679692 sequences (100000005 bp) in 2.624 CPU sec, 2.658 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283195, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (345.14, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679642 reads in 147.146 CPU sec, 4.633 real sec
[M::process] read 678846 sequences (100000119 bp) in 1.910 CPU sec, 1.918 real sec...
sorting block 52, #reads = 1083350 mem = 536877495 peak = 1145806848
[M::mem_process_seqs] Processed 679272 reads in 98.312 CPU sec, 3.097 real sec
sorting block 53, #reads = 1082140 mem = 536874644 peak = 1158070272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283219, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (342.28, 161.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679060 sequences (100000043 bp) in 2.354 CPU sec, 2.378 real sec...
[M::process] read 678370 sequences (100000191 bp) in 1.450 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283462, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (348.99, 163.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679692 reads in 143.905 CPU sec, 4.555 real sec
[M::mem_process_seqs] Processed 678846 reads in 94.096 CPU sec, 2.974 real sec
sorting block 54, #reads = 1083617 mem = 536883369 peak = 1158070272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283032, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (347.92, 163.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678610 sequences (100000202 bp) in 2.513 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282294, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.49, 161.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679060 reads in 145.146 CPU sec, 4.575 real sec
[M::process] read 678202 sequences (100000024 bp) in 1.910 CPU sec, 1.909 real sec...
[M::mem_process_seqs] Processed 678370 reads in 95.963 CPU sec, 3.013 real sec
sorting block 55, #reads = 1084282 mem = 536879222 peak = 1158070272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283649, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (351.75, 164.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678348 sequences (100000209 bp) in 2.550 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282665, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (354.69, 165.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678610 reads in 143.911 CPU sec, 4.539 real sec
[M::process] read 678212 sequences (100000215 bp) in 1.968 CPU sec, 1.965 real sec...
[M::mem_process_seqs] Processed 678202 reads in 96.257 CPU sec, 3.024 real sec
sorting block 56, #reads = 1083445 mem = 536878798 peak = 1158070272
[M::process] read 677924 sequences (100000059 bp) in 2.542 CPU sec, 2.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283265, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (353.86, 164.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678212 reads in 81.509 CPU sec, 2.543 real sec
[M::process] read 678050 sequences (100000128 bp) in 1.928 CPU sec, 1.932 real sec...
sorting block 57, #reads = 1082758 mem = 536877257 peak = 1158070272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283274, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (353.87, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282401, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (354.64, 164.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678348 reads in 228.401 CPU sec, 7.203 real sec
[M::process] read 678326 sequences (100000089 bp) in 2.164 CPU sec, 2.178 real sec...
sorting block 58, #reads = 1083047 mem = 536878641 peak = 1175498752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283135, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (354.73, 164.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678722 sequences (100000208 bp) in 2.116 CPU sec, 2.123 real sec...
[M::mem_process_seqs] Processed 678050 reads in 82.970 CPU sec, 2.616 real sec
[M::mem_process_seqs] Processed 677924 reads in 222.484 CPU sec, 7.028 real sec
sorting block 59, #reads = 1082662 mem = 536878773 peak = 1175498752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282804, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (348.55, 161.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678598 sequences (100000111 bp) in 2.374 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282563, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 321, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (339.60, 157.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678326 reads in 166.241 CPU sec, 5.238 real sec
[M::mem_process_seqs] Processed 678722 reads in 99.430 CPU sec, 3.118 real sec
[M::process] read 678666 sequences (100000031 bp) in 2.111 CPU sec, 2.132 real sec...
sorting block 60, #reads = 1081680 mem = 536880154 peak = 1175498752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282605, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 325, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (343.36, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677674 sequences (100000046 bp) in 2.529 CPU sec, 2.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282474, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (345.30, 161.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678598 reads in 147.218 CPU sec, 4.654 real sec
[M::process] read 677280 sequences (100000100 bp) in 2.017 CPU sec, 2.024 real sec...
[M::mem_process_seqs] Processed 678666 reads in 98.102 CPU sec, 3.077 real sec
sorting block 61, #reads = 1082569 mem = 536879968 peak = 1175498752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282694, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (352.63, 162.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677534 sequences (100000127 bp) in 2.162 CPU sec, 2.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282344, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (353.91, 162.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677598 sequences (100000122 bp) in 1.900 CPU sec, 1.904 real sec...
[M::mem_process_seqs] Processed 677674 reads in 149.409 CPU sec, 4.723 real sec
sorting block 62, #reads = 1082536 mem = 536880975 peak = 1175498752
[M::mem_process_seqs] Processed 677280 reads in 99.000 CPU sec, 3.117 real sec
sorting block 63, #reads = 1082386 mem = 536878839 peak = 1216671744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282604, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (351.82, 161.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677096 sequences (100000228 bp) in 2.620 CPU sec, 2.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282678, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.74, 161.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677534 reads in 144.933 CPU sec, 4.562 real sec
[M::process] read 677332 sequences (100000245 bp) in 1.910 CPU sec, 1.915 real sec...
[M::mem_process_seqs] Processed 677598 reads in 96.002 CPU sec, 3.024 real sec
sorting block 64, #reads = 1081032 mem = 536878489 peak = 1216671744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282385, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.58, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677606 sequences (100000019 bp) in 2.349 CPU sec, 2.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282265, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (353.03, 163.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677096 reads in 145.499 CPU sec, 4.589 real sec
[M::process] read 677652 sequences (100000100 bp) in 1.930 CPU sec, 1.928 real sec...
[M::mem_process_seqs] Processed 677332 reads in 96.938 CPU sec, 3.045 real sec
sorting block 65, #reads = 1082380 mem = 536879982 peak = 1216671744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282232, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (352.88, 163.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677428 sequences (100000187 bp) in 2.416 CPU sec, 2.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282784, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (351.64, 162.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677954 sequences (100000288 bp) in 1.960 CPU sec, 1.953 real sec...
[M::mem_process_seqs] Processed 677606 reads in 147.549 CPU sec, 4.650 real sec
sorting block 66, #reads = 1081700 mem = 536878402 peak = 1216671744
[M::mem_process_seqs] Processed 677652 reads in 97.536 CPU sec, 3.068 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282164, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.17, 162.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677490 sequences (100000185 bp) in 2.377 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282800, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (349.20, 162.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677428 reads in 147.142 CPU sec, 4.646 real sec
[M::process] read 676934 sequences (100000089 bp) in 1.930 CPU sec, 1.929 real sec...
sorting block 67, #reads = 1081918 mem = 536879502 peak = 1216671744
[M::mem_process_seqs] Processed 677954 reads in 99.291 CPU sec, 3.124 real sec
sorting block 68, #reads = 1080625 mem = 536879616 peak = 1223094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282776, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (355.03, 165.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677014 sequences (100000055 bp) in 2.227 CPU sec, 2.273 real sec...
[M::process] read 681680 sequences (100000124 bp) in 1.500 CPU sec, 1.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282296, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 344, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (361.15, 167.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677490 reads in 145.912 CPU sec, 4.617 real sec
[M::mem_process_seqs] Processed 676934 reads in 97.385 CPU sec, 3.090 real sec
sorting block 69, #reads = 1081354 mem = 536879592 peak = 1223094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282535, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (358.00, 165.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681386 sequences (100000188 bp) in 2.062 CPU sec, 2.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283609, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 309, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (330.78, 158.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680886 sequences (100000293 bp) in 1.890 CPU sec, 1.896 real sec...
[M::mem_process_seqs] Processed 677014 reads in 152.675 CPU sec, 4.831 real sec
sorting block 70, #reads = 1079142 mem = 536878874 peak = 1223094272
[M::mem_process_seqs] Processed 681680 reads in 102.476 CPU sec, 3.234 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282913, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 313, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (334.23, 159.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681144 sequences (100000150 bp) in 2.528 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282673, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (333.01, 159.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680828 sequences (100000236 bp) in 1.910 CPU sec, 1.911 real sec...
[M::mem_process_seqs] Processed 680886 reads in 149.484 CPU sec, 4.708 real sec
sorting block 71, #reads = 1084725 mem = 536880390 peak = 1223094272
[M::mem_process_seqs] Processed 681386 reads in 165.799 CPU sec, 5.240 real sec
sorting block 72, #reads = 1086007 mem = 536876743 peak = 1223094272
[M::process] read 680630 sequences (100000098 bp) in 2.273 CPU sec, 2.317 real sec...
[M::process] read 680540 sequences (100000299 bp) in 1.540 CPU sec, 1.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283254, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (333.32, 157.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680828 reads in 80.603 CPU sec, 2.539 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283606, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (333.58, 159.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282457, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (336.11, 159.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680880 sequences (100000166 bp) in 1.839 CPU sec, 1.843 real sec...
[M::mem_process_seqs] Processed 681144 reads in 224.768 CPU sec, 7.105 real sec
sorting block 73, #reads = 1085711 mem = 536879164 peak = 1223094272
[M::mem_process_seqs] Processed 680630 reads in 111.287 CPU sec, 3.538 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283533, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (337.84, 160.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679926 sequences (100000040 bp) in 2.626 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283307, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (336.15, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680540 reads in 143.527 CPU sec, 4.535 real sec
sorting block 74, #reads = 1085657 mem = 536877356 peak = 1223094272
[M::process] read 680068 sequences (100000086 bp) in 2.037 CPU sec, 2.046 real sec...
[M::mem_process_seqs] Processed 680880 reads in 95.000 CPU sec, 2.980 real sec
sorting block 75, #reads = 1084581 mem = 536877699 peak = 1223094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283542, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (341.20, 161.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679698 sequences (100000135 bp) in 2.276 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283025, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (341.75, 162.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679876 sequences (100000239 bp) in 1.540 CPU sec, 1.537 real sec...
[M::mem_process_seqs] Processed 679926 reads in 145.915 CPU sec, 4.620 real sec
[M::mem_process_seqs] Processed 680068 reads in 96.979 CPU sec, 3.057 real sec
sorting block 76, #reads = 1084679 mem = 536878965 peak = 1223094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282654, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (342.27, 161.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679324 sequences (100000129 bp) in 2.230 CPU sec, 2.278 real sec...
[M::process] read 679218 sequences (100000089 bp) in 1.620 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283440, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (342.07, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679698 reads in 147.818 CPU sec, 4.680 real sec
[M::mem_process_seqs] Processed 679876 reads in 97.548 CPU sec, 3.092 real sec
sorting block 77, #reads = 1084505 mem = 536878721 peak = 1223094272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283448, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (345.94, 162.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678788 sequences (100000205 bp) in 2.260 CPU sec, 2.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282772, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (347.54, 163.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678850 sequences (100000051 bp) in 1.920 CPU sec, 1.917 real sec...
[M::mem_process_seqs] Processed 679324 reads in 147.464 CPU sec, 4.655 real sec
sorting block 78, #reads = 1084516 mem = 536878691 peak = 1223094272
[M::mem_process_seqs] Processed 679218 reads in 98.047 CPU sec, 3.084 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282628, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (348.72, 163.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678600 sequences (100000231 bp) in 2.271 CPU sec, 2.325 real sec...
[M::process] read 678532 sequences (100000065 bp) in 1.460 CPU sec, 1.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282503, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (349.63, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678788 reads in 148.545 CPU sec, 4.702 real sec
sorting block 79, #reads = 1083669 mem = 536880646 peak = 1223094272
[M::mem_process_seqs] Processed 678850 reads in 99.565 CPU sec, 3.154 real sec
sorting block 80, #reads = 1084015 mem = 536880119 peak = 1227968512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282234, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (351.56, 165.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678564 sequences (100000085 bp) in 2.336 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283038, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.97, 164.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677976 sequences (100000277 bp) in 1.900 CPU sec, 1.901 real sec...
[M::mem_process_seqs] Processed 678600 reads in 151.583 CPU sec, 4.790 real sec
[M::mem_process_seqs] Processed 678532 reads in 99.705 CPU sec, 3.137 real sec
sorting block 81, #reads = 1082855 mem = 536880452 peak = 1227968512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283209, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (352.38, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678008 sequences (100000007 bp) in 2.547 CPU sec, 2.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282693, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 338, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (355.34, 165.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678564 reads in 147.352 CPU sec, 4.649 real sec
[M::process] read 677996 sequences (100000110 bp) in 1.929 CPU sec, 1.932 real sec...
[M::mem_process_seqs] Processed 677976 reads in 96.964 CPU sec, 3.046 real sec
sorting block 82, #reads = 1084498 mem = 536880296 peak = 1227968512
[M::process] read 677978 sequences (100000135 bp) in 2.009 CPU sec, 2.051 real sec...
[M::process] read 677918 sequences (100000005 bp) in 1.470 CPU sec, 1.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283034, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (353.19, 163.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677996 reads in 82.614 CPU sec, 2.613 real sec
sorting block 83, #reads = 1084164 mem = 536877787 peak = 1227968512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282354, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (353.12, 164.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282591, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.31, 161.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678008 reads in 227.526 CPU sec, 7.204 real sec
[M::process] read 677556 sequences (100000117 bp) in 2.263 CPU sec, 2.276 real sec...
[M::mem_process_seqs] Processed 677978 reads in 113.944 CPU sec, 3.611 real sec
sorting block 84, #reads = 1082912 mem = 536879059 peak = 1227968512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282333, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.90, 162.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677210 sequences (100000127 bp) in 2.590 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282414, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (352.35, 161.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677918 reads in 150.854 CPU sec, 4.759 real sec
[M::process] read 677228 sequences (100000019 bp) in 1.860 CPU sec, 1.853 real sec...
sorting block 85, #reads = 1082123 mem = 536880470 peak = 1227968512
[M::mem_process_seqs] Processed 677556 reads in 99.406 CPU sec, 3.123 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282536, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (354.69, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677080 sequences (100000190 bp) in 2.576 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281726, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (353.30, 160.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677066 sequences (100000055 bp) in 1.890 CPU sec, 1.890 real sec...
[M::mem_process_seqs] Processed 677210 reads in 147.184 CPU sec, 4.633 real sec
sorting block 86, #reads = 1081675 mem = 536889311 peak = 1227968512
[M::mem_process_seqs] Processed 677228 reads in 96.995 CPU sec, 3.056 real sec
sorting block 87, #reads = 1081538 mem = 536878400 peak = 1227968512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281941, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 339, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (353.58, 160.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676914 sequences (100000106 bp) in 2.269 CPU sec, 2.310 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282399, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (352.52, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676946 sequences (100000043 bp) in 1.590 CPU sec, 1.593 real sec...
[M::mem_process_seqs] Processed 677080 reads in 144.628 CPU sec, 4.575 real sec
[M::mem_process_seqs] Processed 677066 reads in 94.629 CPU sec, 2.996 real sec
sorting block 88, #reads = 1081296 mem = 536878520 peak = 1227968512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283100, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 339, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (353.65, 160.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676990 sequences (100000261 bp) in 2.098 CPU sec, 2.154 real sec...
[M::process] read 677046 sequences (100000097 bp) in 1.630 CPU sec, 1.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282310, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (354.77, 161.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676914 reads in 146.980 CPU sec, 4.656 real sec
[M::mem_process_seqs] Processed 676946 reads in 98.061 CPU sec, 3.101 real sec
sorting block 89, #reads = 1081137 mem = 536880030 peak = 1227968512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282050, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (352.26, 160.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677156 sequences (100000238 bp) in 2.364 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282288, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (351.78, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677070 sequences (100000015 bp) in 1.920 CPU sec, 1.917 real sec...
[M::mem_process_seqs] Processed 676990 reads in 146.700 CPU sec, 4.628 real sec
sorting block 90, #reads = 1081466 mem = 536878890 peak = 1227968512
[M::mem_process_seqs] Processed 677046 reads in 98.089 CPU sec, 3.092 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282501, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (349.24, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677082 sequences (100000248 bp) in 2.574 CPU sec, 2.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282692, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 335, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (350.69, 159.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676894 sequences (100000262 bp) in 1.700 CPU sec, 1.693 real sec...
[M::mem_process_seqs] Processed 677156 reads in 147.207 CPU sec, 4.642 real sec
sorting block 91, #reads = 1080754 mem = 536881296 peak = 1227968512
[M::mem_process_seqs] Processed 677070 reads in 98.793 CPU sec, 3.117 real sec
sorting block 92, #reads = 1080588 mem = 536879706 peak = 1227968512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281983, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (349.66, 158.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676918 sequences (100000012 bp) in 2.492 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282281, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 336, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (351.82, 160.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685416 sequences (100000299 bp) in 1.720 CPU sec, 1.717 real sec...
[M::mem_process_seqs] Processed 677082 reads in 153.314 CPU sec, 4.845 real sec
[M::mem_process_seqs] Processed 676894 reads in 101.884 CPU sec, 3.220 real sec
sorting block 93, #reads = 1080628 mem = 536882895 peak = 1227968512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282329, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (355.84, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683152 sequences (100000172 bp) in 2.363 CPU sec, 2.397 real sec...
[M::process] read 683156 sequences (100000035 bp) in 1.570 CPU sec, 1.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285224, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 281, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (308.78, 152.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676918 reads in 157.653 CPU sec, 4.990 real sec
[M::mem_process_seqs] Processed 685416 reads in 109.923 CPU sec, 3.477 real sec
sorting block 94, #reads = 1078529 mem = 536879275 peak = 1227968512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284716, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (324.97, 159.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683124 sequences (100000078 bp) in 2.482 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284650, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 300, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (326.26, 160.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682398 sequences (100000073 bp) in 2.010 CPU sec, 2.006 real sec...
[M::mem_process_seqs] Processed 683152 reads in 157.835 CPU sec, 4.980 real sec
sorting block 95, #reads = 1080298 mem = 536881596 peak = 1227968512
[M::mem_process_seqs] Processed 683156 reads in 103.769 CPU sec, 3.266 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285018, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 301, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (325.60, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681188 sequences (100000086 bp) in 2.247 CPU sec, 2.275 real sec...
[M::process] read 680876 sequences (100000211 bp) in 1.540 CPU sec, 1.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284800, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 304, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (328.90, 159.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683124 reads in 151.701 CPU sec, 4.801 real sec
sorting block 96, #reads = 1082080 mem = 536879052 peak = 1227968512
[M::mem_process_seqs] Processed 682398 reads in 101.135 CPU sec, 3.201 real sec
sorting block 97, #reads = 1081644 mem = 536881347 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284493, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (336.18, 161.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680458 sequences (100000190 bp) in 2.046 CPU sec, 2.078 real sec...
[M::process] read 680110 sequences (100000093 bp) in 1.470 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283980, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (338.70, 162.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681188 reads in 146.062 CPU sec, 4.628 real sec
[M::mem_process_seqs] Processed 680876 reads in 96.062 CPU sec, 3.050 real sec
sorting block 98, #reads = 1083028 mem = 536883485 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283808, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (341.38, 162.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679766 sequences (100000026 bp) in 2.070 CPU sec, 2.102 real sec...
[M::process] read 679628 sequences (100000009 bp) in 1.490 CPU sec, 1.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283952, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (345.02, 164.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680458 reads in 147.815 CPU sec, 4.688 real sec
[M::mem_process_seqs] Processed 680110 reads in 96.814 CPU sec, 3.074 real sec
sorting block 99, #reads = 1081990 mem = 536878003 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283565, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (346.58, 164.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679390 sequences (100000281 bp) in 2.616 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283799, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 330, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (348.40, 165.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679766 reads in 150.298 CPU sec, 4.738 real sec
[M::process] read 679470 sequences (100000279 bp) in 1.950 CPU sec, 1.948 real sec...
sorting block 100, #reads = 1082731 mem = 536877584 peak = 1248706560
[M::mem_process_seqs] Processed 679628 reads in 99.380 CPU sec, 3.127 real sec
sorting block 101, #reads = 1081666 mem = 536879681 peak = 1248706560
[M::process] read 678680 sequences (100000269 bp) in 2.668 CPU sec, 2.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283582, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (347.24, 163.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678608 sequences (100000116 bp) in 1.970 CPU sec, 1.971 real sec...
[M::mem_process_seqs] Processed 679470 reads in 90.429 CPU sec, 2.838 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283139, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (347.15, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283185, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 337, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (353.75, 166.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679390 reads in 247.397 CPU sec, 7.806 real sec
[M::process] read 679014 sequences (100000157 bp) in 1.969 CPU sec, 1.978 real sec...
sorting block 102, #reads = 1080373 mem = 536879900 peak = 1248706560
[M::mem_process_seqs] Processed 678680 reads in 126.157 CPU sec, 4.001 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283434, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 340, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (355.78, 166.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678826 sequences (100000200 bp) in 2.757 CPU sec, 2.811 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283066, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 337, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (353.87, 167.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678406 sequences (100000017 bp) in 1.970 CPU sec, 1.968 real sec...
[M::mem_process_seqs] Processed 678608 reads in 161.218 CPU sec, 5.085 real sec
sorting block 103, #reads = 1079018 mem = 536877743 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282918, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 334, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (352.75, 166.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678212 sequences (100000030 bp) in 2.299 CPU sec, 2.308 real sec...
[M::mem_process_seqs] Processed 678826 reads in 89.324 CPU sec, 2.810 real sec
sorting block 104, #reads = 1081192 mem = 536881970 peak = 1248706560
[M::mem_process_seqs] Processed 679014 reads in 226.330 CPU sec, 7.147 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283299, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (350.50, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678250 sequences (100000228 bp) in 2.409 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282984, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (350.85, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678406 reads in 181.126 CPU sec, 5.719 real sec
[M::process] read 677854 sequences (100000162 bp) in 1.984 CPU sec, 1.988 real sec...
sorting block 105, #reads = 1079506 mem = 536883938 peak = 1248706560
[M::mem_process_seqs] Processed 678212 reads in 109.508 CPU sec, 3.447 real sec
sorting block 106, #reads = 1078859 mem = 536882454 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283277, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.43, 163.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677866 sequences (100000293 bp) in 2.566 CPU sec, 2.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283009, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (352.77, 163.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677568 sequences (100000187 bp) in 1.970 CPU sec, 1.972 real sec...
[M::mem_process_seqs] Processed 678250 reads in 164.790 CPU sec, 5.199 real sec
[M::mem_process_seqs] Processed 677854 reads in 107.076 CPU sec, 3.379 real sec
sorting block 107, #reads = 1078774 mem = 536878274 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283126, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (351.24, 162.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677834 sequences (100000065 bp) in 2.600 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282639, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (352.64, 163.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678302 sequences (100000070 bp) in 1.970 CPU sec, 1.973 real sec...
[M::mem_process_seqs] Processed 677866 reads in 157.396 CPU sec, 4.961 real sec
[M::mem_process_seqs] Processed 677568 reads in 105.580 CPU sec, 3.332 real sec
sorting block 108, #reads = 1079461 mem = 536879730 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282558, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.47, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678132 sequences (100000168 bp) in 2.504 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282816, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (349.10, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678456 sequences (100000096 bp) in 2.040 CPU sec, 2.032 real sec...
[M::mem_process_seqs] Processed 677834 reads in 157.019 CPU sec, 4.944 real sec
[M::mem_process_seqs] Processed 678302 reads in 175.167 CPU sec, 5.538 real sec
sorting block 109, #reads = 1079418 mem = 536878817 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283279, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (350.04, 162.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678162 sequences (100000296 bp) in 2.668 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283253, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (347.24, 161.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678050 sequences (100000085 bp) in 2.000 CPU sec, 1.999 real sec...
[M::mem_process_seqs] Processed 678132 reads in 164.000 CPU sec, 5.176 real sec
sorting block 110, #reads = 1078538 mem = 536881253 peak = 1248706560
[M::mem_process_seqs] Processed 678456 reads in 108.580 CPU sec, 3.420 real sec
sorting block 111, #reads = 1078883 mem = 536879093 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283212, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (348.75, 162.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677968 sequences (100000030 bp) in 2.667 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282747, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (350.07, 163.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677722 sequences (100000098 bp) in 2.010 CPU sec, 2.015 real sec...
[M::mem_process_seqs] Processed 678162 reads in 163.452 CPU sec, 5.153 real sec
[M::mem_process_seqs] Processed 678050 reads in 108.502 CPU sec, 3.425 real sec
sorting block 112, #reads = 1078722 mem = 536880061 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283030, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.01, 163.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677770 sequences (100000047 bp) in 2.529 CPU sec, 2.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282453, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (350.21, 162.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677854 sequences (100000205 bp) in 1.960 CPU sec, 1.966 real sec...
[M::mem_process_seqs] Processed 677968 reads in 160.192 CPU sec, 5.054 real sec
[M::mem_process_seqs] Processed 677722 reads in 106.137 CPU sec, 3.351 real sec
sorting block 113, #reads = 1078736 mem = 536875659 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283065, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.50, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678034 sequences (100000287 bp) in 2.557 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282699, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (351.52, 163.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685328 sequences (100000286 bp) in 2.100 CPU sec, 2.095 real sec...
[M::mem_process_seqs] Processed 677770 reads in 160.444 CPU sec, 5.053 real sec
sorting block 114, #reads = 1077859 mem = 536880259 peak = 1248706560
[M::mem_process_seqs] Processed 677854 reads in 109.725 CPU sec, 3.463 real sec
[M::process] read 685662 sequences (100000270 bp) in 2.441 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282914, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (350.09, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684158 sequences (100000201 bp) in 1.850 CPU sec, 1.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285589, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (310.52, 150.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678034 reads in 175.373 CPU sec, 5.545 real sec
sorting block 115, #reads = 1078298 mem = 536878589 peak = 1248706560
[M::mem_process_seqs] Processed 685328 reads in 195.991 CPU sec, 6.214 real sec
sorting block 116, #reads = 1075816 mem = 536879789 peak = 1248706560
[M::process] read 684294 sequences (100000188 bp) in 2.243 CPU sec, 2.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286096, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (308.02, 149.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684256 sequences (100000262 bp) in 1.602 CPU sec, 1.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285457, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (315.93, 152.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285321, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (315.02, 151.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685662 reads in 250.167 CPU sec, 7.974 real sec
[M::mem_process_seqs] Processed 684158 reads in 190.547 CPU sec, 6.098 real sec
[M::mem_process_seqs] Processed 684294 reads in 210.492 CPU sec, 6.738 real sec
sorting block 117, #reads = 1077758 mem = 536880909 peak = 1248706560
[M::process] read 683096 sequences (100000282 bp) in 2.429 CPU sec, 2.500 real sec...
sorting block 118, #reads = 1077439 mem = 536880513 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285579, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (315.10, 149.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682556 sequences (100000007 bp) in 1.774 CPU sec, 1.798 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285303, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 298, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (318.83, 150.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682160 sequences (100000294 bp) in 1.580 CPU sec, 1.583 real sec...
[M::mem_process_seqs] Processed 684256 reads in 175.625 CPU sec, 5.566 real sec
[M::mem_process_seqs] Processed 683096 reads in 114.941 CPU sec, 3.632 real sec
sorting block 119, #reads = 1076836 mem = 536882132 peak = 1248706560
[M::process] read 681472 sequences (100000037 bp) in 2.254 CPU sec, 2.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285069, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 304, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (323.05, 152.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680960 sequences (100000193 bp) in 1.740 CPU sec, 1.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284454, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 311, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (330.16, 155.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284625, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 307, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (326.23, 153.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682556 reads in 235.152 CPU sec, 7.487 real sec
[M::mem_process_seqs] Processed 681472 reads in 178.246 CPU sec, 5.694 real sec
sorting block 120, #reads = 1076832 mem = 536878662 peak = 1248706560
[M::mem_process_seqs] Processed 682160 reads in 196.731 CPU sec, 6.293 real sec
sorting block 121, #reads = 1076654 mem = 536879289 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284203, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (331.28, 155.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680660 sequences (100000043 bp) in 2.764 CPU sec, 2.867 real sec...
[M::mem_process_seqs] Processed 680960 reads in 94.810 CPU sec, 3.088 real sec
[M::process] read 680344 sequences (100000168 bp) in 2.417 CPU sec, 2.471 real sec...
[M::process] read 680230 sequences (100000185 bp) in 2.010 CPU sec, 2.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283837, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (336.15, 157.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680344 reads in 93.056 CPU sec, 2.907 real sec
sorting block 122, #reads = 1078209 mem = 536876840 peak = 1248706560
[M::process] read 679986 sequences (100000000 bp) in 1.867 CPU sec, 1.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284237, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (333.69, 155.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284760, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 321, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (338.34, 157.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679868 sequences (100000035 bp) in 1.674 CPU sec, 1.679 real sec...
[M::mem_process_seqs] Processed 680660 reads in 259.975 CPU sec, 8.168 real sec
[M::mem_process_seqs] Processed 680230 reads in 129.038 CPU sec, 4.079 real sec
sorting block 123, #reads = 1077942 mem = 536879622 peak = 1248706560
[M::process] read 679652 sequences (100000079 bp) in 1.865 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284370, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (339.99, 159.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679596 sequences (100000059 bp) in 1.530 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283739, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (341.31, 160.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283556, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (344.80, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679986 reads in 228.783 CPU sec, 7.370 real sec
sorting block 124, #reads = 1079154 mem = 536878664 peak = 1248706560
[M::mem_process_seqs] Processed 679868 reads in 171.396 CPU sec, 5.481 real sec
[M::mem_process_seqs] Processed 679652 reads in 189.721 CPU sec, 6.080 real sec
sorting block 125, #reads = 1079039 mem = 536877782 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283776, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (342.46, 160.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679208 sequences (100000240 bp) in 2.854 CPU sec, 2.957 real sec...
[M::mem_process_seqs] Processed 679596 reads in 92.754 CPU sec, 3.013 real sec
sorting block 126, #reads = 1079842 mem = 536879502 peak = 1248706560
[M::process] read 678586 sequences (100000119 bp) in 2.226 CPU sec, 2.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283830, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (342.04, 159.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678590 sequences (100000220 bp) in 1.970 CPU sec, 1.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283715, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (347.49, 161.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679208 reads in 162.173 CPU sec, 5.080 real sec
[M::mem_process_seqs] Processed 678586 reads in 107.548 CPU sec, 3.368 real sec
[M::process] read 678196 sequences (100000163 bp) in 2.191 CPU sec, 2.205 real sec...
sorting block 127, #reads = 1078465 mem = 536883802 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283536, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (348.61, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678274 sequences (100000142 bp) in 2.232 CPU sec, 2.279 real sec...
[M::process] read 677836 sequences (100000133 bp) in 1.440 CPU sec, 1.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283193, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (351.01, 163.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678590 reads in 162.186 CPU sec, 5.144 real sec
sorting block 128, #reads = 1079016 mem = 536879464 peak = 1248706560
[M::mem_process_seqs] Processed 678196 reads in 109.171 CPU sec, 3.467 real sec
[M::process] read 678022 sequences (100000032 bp) in 2.305 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283347, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (350.84, 163.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677948 sequences (100000104 bp) in 1.980 CPU sec, 1.980 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283478, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (354.54, 166.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678274 reads in 163.202 CPU sec, 5.149 real sec
sorting block 129, #reads = 1078583 mem = 536879761 peak = 1248706560
[M::mem_process_seqs] Processed 677836 reads in 181.329 CPU sec, 5.736 real sec
sorting block 130, #reads = 1079070 mem = 536877791 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283061, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (352.23, 165.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678054 sequences (100000095 bp) in 2.349 CPU sec, 2.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282442, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.75, 166.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677746 sequences (100000105 bp) in 1.960 CPU sec, 1.956 real sec...
[M::mem_process_seqs] Processed 678022 reads in 156.542 CPU sec, 4.938 real sec
[M::mem_process_seqs] Processed 677948 reads in 104.873 CPU sec, 3.313 real sec
sorting block 131, #reads = 1079387 mem = 536878447 peak = 1248706560
[M::process] read 678196 sequences (100000191 bp) in 2.299 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282912, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (353.91, 167.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677726 sequences (100000142 bp) in 2.000 CPU sec, 1.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282941, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (356.34, 167.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678054 reads in 163.114 CPU sec, 5.148 real sec
[M::mem_process_seqs] Processed 677746 reads in 182.086 CPU sec, 5.761 real sec
sorting block 132, #reads = 1079166 mem = 536878256 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282431, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (352.61, 166.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677738 sequences (100000265 bp) in 2.586 CPU sec, 2.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282715, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (357.33, 169.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677544 sequences (100000217 bp) in 2.020 CPU sec, 2.020 real sec...
[M::mem_process_seqs] Processed 678196 reads in 164.124 CPU sec, 5.174 real sec
sorting block 133, #reads = 1078075 mem = 536879037 peak = 1248706560
[M::mem_process_seqs] Processed 677726 reads in 106.696 CPU sec, 3.371 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282541, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (359.79, 171.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682224 sequences (100000165 bp) in 2.645 CPU sec, 2.676 real sec...
[M::process] read 683618 sequences (100000215 bp) in 1.980 CPU sec, 1.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282303, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (359.21, 171.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677738 reads in 168.395 CPU sec, 5.313 real sec
sorting block 134, #reads = 1077345 mem = 536879646 peak = 1248706560
[M::mem_process_seqs] Processed 677544 reads in 116.398 CPU sec, 3.681 real sec
sorting block 135, #reads = 1075253 mem = 536880431 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284168, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 683804 sequences (100000214 bp) in 2.613 CPU sec, 2.644 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (201, 306, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (329.53, 160.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683136 sequences (100000208 bp) in 1.990 CPU sec, 1.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284974, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (321.87, 157.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682224 reads in 176.702 CPU sec, 5.581 real sec
[M::mem_process_seqs] Processed 683618 reads in 109.745 CPU sec, 3.476 real sec
sorting block 136, #reads = 1071623 mem = 536877095 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285329, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (321.83, 158.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682568 sequences (100000050 bp) in 2.453 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284817, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (322.72, 156.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681774 sequences (100000206 bp) in 1.990 CPU sec, 1.993 real sec...
[M::mem_process_seqs] Processed 683804 reads in 158.738 CPU sec, 5.011 real sec
[M::mem_process_seqs] Processed 683136 reads in 104.754 CPU sec, 3.302 real sec
sorting block 137, #reads = 1082670 mem = 536882868 peak = 1248706560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285182, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 303, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (326.60, 158.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681732 sequences (100000052 bp) in 2.652 CPU sec, 2.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284491, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 310, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (333.52, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681140 sequences (100000035 bp) in 1.970 CPU sec, 1.971 real sec...
[M::mem_process_seqs] Processed 682568 reads in 153.347 CPU sec, 4.830 real sec
sorting block 138, #reads = 1080982 mem = 536878120 peak = 1248706560
[M::mem_process_seqs] Processed 681774 reads in 100.711 CPU sec, 3.168 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284513, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 310, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (333.39, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680270 sequences (100000286 bp) in 2.486 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284122, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (334.65, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680172 sequences (100000255 bp) in 1.970 CPU sec, 1.966 real sec...
[M::mem_process_seqs] Processed 681732 reads in 151.492 CPU sec, 4.773 real sec
sorting block 139, #reads = 1082688 mem = 536880433 peak = 1248706560
[M::mem_process_seqs] Processed 681140 reads in 98.373 CPU sec, 3.101 real sec
sorting block 140, #reads = 1083000 mem = 536877740 peak = 1267736576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283333, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (336.87, 160.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680288 sequences (100000148 bp) in 2.645 CPU sec, 2.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283297, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (338.03, 161.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680270 reads in 147.786 CPU sec, 4.650 real sec
[M::process] read 680246 sequences (100000288 bp) in 1.950 CPU sec, 1.956 real sec...
[M::mem_process_seqs] Processed 680172 reads in 97.867 CPU sec, 3.081 real sec
sorting block 141, #reads = 1082163 mem = 536881764 peak = 1267736576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283318, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (338.00, 161.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679890 sequences (100000139 bp) in 2.622 CPU sec, 2.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284357, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (338.56, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680288 reads in 148.704 CPU sec, 4.691 real sec
[M::process] read 679850 sequences (100000037 bp) in 2.007 CPU sec, 2.004 real sec...
[M::mem_process_seqs] Processed 680246 reads in 98.688 CPU sec, 3.099 real sec
sorting block 142, #reads = 1082146 mem = 536878104 peak = 1267736576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283404, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (337.94, 159.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679802 sequences (100000052 bp) in 2.204 CPU sec, 2.245 real sec...
[M::process] read 679386 sequences (100000260 bp) in 1.470 CPU sec, 1.469 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283817, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (339.19, 158.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679890 reads in 154.385 CPU sec, 5.035 real sec
sorting block 143, #reads = 1082908 mem = 536881507 peak = 1267736576
[M::mem_process_seqs] Processed 679850 reads in 99.631 CPU sec, 3.155 real sec
sorting block 144, #reads = 1081452 mem = 536878238 peak = 1267736576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283555, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (337.94, 158.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679472 sequences (100000082 bp) in 2.325 CPU sec, 2.367 real sec...
[M::process] read 679868 sequences (100000220 bp) in 1.540 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283867, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (340.05, 159.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679802 reads in 155.325 CPU sec, 4.916 real sec
[M::mem_process_seqs] Processed 679386 reads in 100.846 CPU sec, 3.205 real sec
sorting block 145, #reads = 1080605 mem = 536879694 peak = 1267736576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283524, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (340.56, 160.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679890 sequences (100000165 bp) in 2.139 CPU sec, 2.195 real sec...
[M::process] read 680534 sequences (100000209 bp) in 1.500 CPU sec, 1.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283322, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (340.24, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679472 reads in 155.799 CPU sec, 4.940 real sec
[M::mem_process_seqs] Processed 679868 reads in 102.040 CPU sec, 3.239 real sec
sorting block 146, #reads = 1079834 mem = 536880765 peak = 1267736576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283632, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (337.62, 159.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680346 sequences (100000180 bp) in 2.644 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284207, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 313, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (333.89, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680390 sequences (100000151 bp) in 2.010 CPU sec, 2.007 real sec...
[M::mem_process_seqs] Processed 679890 reads in 158.057 CPU sec, 4.978 real sec
sorting block 147, #reads = 1080656 mem = 536878420 peak = 1267736576
[M::mem_process_seqs] Processed 680534 reads in 104.309 CPU sec, 3.293 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283535, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.25, 159.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680016 sequences (100000292 bp) in 2.648 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284409, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (335.09, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679178 sequences (100000123 bp) in 1.980 CPU sec, 1.974 real sec...
[M::mem_process_seqs] Processed 680346 reads in 155.635 CPU sec, 4.900 real sec
sorting block 148, #reads = 1080750 mem = 536879073 peak = 1267736576
[M::mem_process_seqs] Processed 680390 reads in 101.758 CPU sec, 3.211 real sec
sorting block 149, #reads = 1080885 mem = 536879559 peak = 1267736576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283274, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (341.48, 163.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678916 sequences (100000037 bp) in 2.621 CPU sec, 2.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283645, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 328, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (348.72, 166.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678780 sequences (100000227 bp) in 2.010 CPU sec, 2.002 real sec...
[M::mem_process_seqs] Processed 680016 reads in 158.236 CPU sec, 4.985 real sec
[M::mem_process_seqs] Processed 679178 reads in 104.590 CPU sec, 3.302 real sec
sorting block 150, #reads = 1079663 mem = 536878697 peak = 1267736576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283505, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 329, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (349.73, 166.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678586 sequences (100000163 bp) in 2.424 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282763, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 330, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (350.40, 166.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678402 sequences (100000241 bp) in 1.970 CPU sec, 1.970 real sec...
[M::mem_process_seqs] Processed 678916 reads in 156.841 CPU sec, 4.947 real sec
[M::mem_process_seqs] Processed 678780 reads in 104.724 CPU sec, 3.309 real sec
sorting block 151, #reads = 1079223 mem = 536879099 peak = 1267736576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282983, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (350.37, 164.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678110 sequences (100000279 bp) in 2.185 CPU sec, 2.206 real sec...
[M::process] read 678134 sequences (100000039 bp) in 1.540 CPU sec, 1.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282885, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (351.17, 165.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678586 reads in 160.326 CPU sec, 5.152 real sec
[M::mem_process_seqs] Processed 678402 reads in 103.713 CPU sec, 3.299 real sec
sorting block 152, #reads = 1079405 mem = 536880040 peak = 1267736576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283471, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.95, 167.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679036 sequences (100000078 bp) in 2.124 CPU sec, 2.163 real sec...
[M::process] read 677974 sequences (100000044 bp) in 2.030 CPU sec, 2.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283118, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (354.60, 169.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678110 reads in 158.444 CPU sec, 5.005 real sec
sorting block 153, #reads = 1078468 mem = 536878843 peak = 1267736576
[M::mem_process_seqs] Processed 678134 reads in 106.687 CPU sec, 3.377 real sec
sorting block 154, #reads = 1078819 mem = 536876285 peak = 1285292032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283059, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (345.93, 167.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678830 sequences (100000172 bp) in 2.484 CPU sec, 2.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283159, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (354.88, 170.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685948 sequences (100000285 bp) in 2.090 CPU sec, 2.093 real sec...
[M::mem_process_seqs] Processed 679036 reads in 166.323 CPU sec, 5.248 real sec
[M::mem_process_seqs] Processed 677974 reads in 112.724 CPU sec, 3.558 real sec
sorting block 155, #reads = 1077186 mem = 536879553 peak = 1285292032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283059, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (351.40, 169.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685534 sequences (100000043 bp) in 2.709 CPU sec, 2.733 real sec...
[M::process] read 685396 sequences (100000169 bp) in 2.050 CPU sec, 2.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285588, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (308.34, 150.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678830 reads in 187.476 CPU sec, 5.925 real sec
[M::mem_process_seqs] Processed 685948 reads in 124.542 CPU sec, 3.955 real sec
sorting block 156, #reads = 1070541 mem = 536879443 peak = 1285292032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286029, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (308.83, 149.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686120 sequences (100000045 bp) in 2.701 CPU sec, 2.722 real sec...
[M::process] read 684516 sequences (100000200 bp) in 2.090 CPU sec, 2.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285727, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (309.65, 149.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685534 reads in 174.036 CPU sec, 5.488 real sec
sorting block 157, #reads = 1078061 mem = 536880135 peak = 1285292032
[M::mem_process_seqs] Processed 685396 reads in 115.309 CPU sec, 3.650 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286131, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (304.99, 146.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684554 sequences (100000238 bp) in 2.712 CPU sec, 2.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285580, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (312.07, 148.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684652 sequences (100000019 bp) in 2.090 CPU sec, 2.084 real sec...
[M::mem_process_seqs] Processed 686120 reads in 176.347 CPU sec, 5.568 real sec
sorting block 158, #reads = 1079225 mem = 536883106 peak = 1285292032
[M::mem_process_seqs] Processed 684516 reads in 114.735 CPU sec, 3.626 real sec
sorting block 159, #reads = 1076681 mem = 536878509 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285919, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (311.59, 149.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683112 sequences (100000036 bp) in 2.693 CPU sec, 2.706 real sec...
[M::process] read 682404 sequences (100000218 bp) in 2.060 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285308, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (311.41, 147.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684554 reads in 176.902 CPU sec, 5.588 real sec
[M::mem_process_seqs] Processed 684652 reads in 117.827 CPU sec, 3.730 real sec
sorting block 160, #reads = 1077765 mem = 536878873 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284773, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 298, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (318.05, 149.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681702 sequences (100000058 bp) in 2.693 CPU sec, 2.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284901, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 304, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (323.89, 153.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681390 sequences (100000020 bp) in 2.030 CPU sec, 2.029 real sec...
[M::mem_process_seqs] Processed 683112 reads in 167.193 CPU sec, 5.273 real sec
[M::mem_process_seqs] Processed 682404 reads in 109.162 CPU sec, 3.442 real sec
sorting block 161, #reads = 1075323 mem = 536882068 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284803, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 309, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (328.73, 154.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680928 sequences (100000106 bp) in 2.691 CPU sec, 2.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284420, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (331.88, 156.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681164 sequences (100000026 bp) in 2.030 CPU sec, 2.026 real sec...
[M::mem_process_seqs] Processed 681702 reads in 161.451 CPU sec, 5.090 real sec
sorting block 162, #reads = 1079409 mem = 536878860 peak = 1288720384
[M::mem_process_seqs] Processed 681390 reads in 105.933 CPU sec, 3.344 real sec
sorting block 163, #reads = 1078592 mem = 536878502 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284069, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (332.52, 156.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680496 sequences (100000121 bp) in 2.477 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284537, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (334.28, 158.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680680 sequences (100000254 bp) in 2.020 CPU sec, 2.016 real sec...
[M::mem_process_seqs] Processed 680928 reads in 165.188 CPU sec, 5.214 real sec
[M::mem_process_seqs] Processed 681164 reads in 109.196 CPU sec, 3.455 real sec
sorting block 164, #reads = 1078752 mem = 536880166 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283875, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (335.13, 157.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680296 sequences (100000182 bp) in 2.673 CPU sec, 2.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283846, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 318, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (337.17, 159.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680292 sequences (100000294 bp) in 2.030 CPU sec, 2.031 real sec...
[M::mem_process_seqs] Processed 680496 reads in 162.904 CPU sec, 5.133 real sec
[M::mem_process_seqs] Processed 680680 reads in 180.213 CPU sec, 5.697 real sec
sorting block 165, #reads = 1078769 mem = 536877956 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283289, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (340.03, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679754 sequences (100000145 bp) in 2.672 CPU sec, 2.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283910, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (340.90, 161.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679562 sequences (100000151 bp) in 2.000 CPU sec, 2.001 real sec...
[M::mem_process_seqs] Processed 680296 reads in 162.047 CPU sec, 5.111 real sec
sorting block 166, #reads = 1079142 mem = 536878546 peak = 1288720384
[M::mem_process_seqs] Processed 680292 reads in 107.180 CPU sec, 3.378 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283253, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (341.67, 161.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679248 sequences (100000172 bp) in 2.660 CPU sec, 2.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283608, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 325, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (343.22, 161.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679400 sequences (100000177 bp) in 2.020 CPU sec, 2.021 real sec...
[M::mem_process_seqs] Processed 679754 reads in 161.873 CPU sec, 5.105 real sec
sorting block 167, #reads = 1079030 mem = 536879716 peak = 1288720384
[M::mem_process_seqs] Processed 679562 reads in 105.959 CPU sec, 3.344 real sec
sorting block 168, #reads = 1079102 mem = 536881649 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282795, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (342.83, 159.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679098 sequences (100000177 bp) in 2.482 CPU sec, 2.519 real sec...
[M::process] read 678650 sequences (100000296 bp) in 1.930 CPU sec, 1.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283585, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (341.74, 158.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679248 reads in 158.009 CPU sec, 4.984 real sec
[M::mem_process_seqs] Processed 679400 reads in 103.091 CPU sec, 3.264 real sec
sorting block 169, #reads = 1079169 mem = 536878329 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283102, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (345.85, 161.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678412 sequences (100000224 bp) in 2.555 CPU sec, 2.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282617, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (348.96, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677886 sequences (100000073 bp) in 1.570 CPU sec, 1.568 real sec...
[M::mem_process_seqs] Processed 679098 reads in 145.709 CPU sec, 4.601 real sec
[M::mem_process_seqs] Processed 678650 reads in 88.476 CPU sec, 2.794 real sec
sorting block 170, #reads = 1079619 mem = 536879765 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283104, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (351.32, 163.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678090 sequences (100000215 bp) in 1.925 CPU sec, 1.948 real sec...
[M::process] read 677978 sequences (100000294 bp) in 1.410 CPU sec, 1.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282573, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (352.81, 162.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678412 reads in 128.490 CPU sec, 4.068 real sec
sorting block 171, #reads = 1079561 mem = 536878198 peak = 1288720384
[M::mem_process_seqs] Processed 677886 reads in 83.477 CPU sec, 2.642 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282328, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (352.12, 162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678276 sequences (100000072 bp) in 2.074 CPU sec, 2.093 real sec...
[M::process] read 677362 sequences (100000300 bp) in 1.490 CPU sec, 1.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282729, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (350.72, 162.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678090 reads in 143.903 CPU sec, 4.563 real sec
sorting block 172, #reads = 1079484 mem = 536878426 peak = 1288720384
[M::mem_process_seqs] Processed 677978 reads in 96.003 CPU sec, 3.039 real sec
sorting block 173, #reads = 1079791 mem = 536877917 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282977, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (347.04, 160.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677576 sequences (100000192 bp) in 2.374 CPU sec, 2.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282627, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (352.48, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 660290 sequences (97348451 bp) in 1.890 CPU sec, 1.890 real sec...
[M::mem_process_seqs] Processed 678276 reads in 150.128 CPU sec, 4.733 real sec
[M::mem_process_seqs] Processed 677362 reads in 98.169 CPU sec, 3.097 real sec
sorting block 174, #reads = 1079413 mem = 536884278 peak = 1288720384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282347, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (352.42, 162.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 275910, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (346.41, 161.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677576 reads in 146.620 CPU sec, 4.734 real sec
[M::mem_process_seqs] Processed 660290 reads in 99.794 CPU sec, 3.222 real sec
sorting block 175, #reads = 1079900 mem = 536881861 peak = 1288720384
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 190177238
overall: 70051737600 mem 25596.193 user 204.795 sys 839.776 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cVw-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 839.807 sec; CPU: 25800.994 sec
sorting block 176, #reads = 736946 mem = 367495034 peak = 1313931264
uniq header count 1
merging 177 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 10518505717
output reads: 190999239
bam_mem_sort: 177 calls 96.135 user 0.314 sys 98.307 real
bam_write: 177 calls 62.750 user 2.395 sys 95.132 real
execute: 1 calls 48.648 user 1.081 sys 919.676 real
merge_files: 1 calls 48.623 user 1.053 sys 79.073 real
parse_chunk: 2827 calls 163.634 user 30.351 sys 212.740 real
read_chunk: 7617 calls 64.343 user 48.343 sys 816.723 real
sort_block: 1 calls 42.084 user 47.986 sys 785.840 real
write_chunk: 4788 calls 48.220 user 0.743 sys 78.108 real
overall: 3409076224 mem 3089.988 user 95.112 sys 919.694 real
ls: cannot access '*.cram': No such file or directory