This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_dm4-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2795.016 CPU sec, 101.978 real sec [M::process] read 759646 sequences (100000187 bp) in 1.204 CPU sec, 1.206 real sec... [M::process] read 756558 sequences (100000094 bp) in 1.871 CPU sec, 1.934 real sec... [M::process] read 756336 sequences (100000130 bp) in 1.996 CPU sec, 2.080 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 304934, 27, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 339, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2205) [M::mem_pestat] mean and std.dev: (524.55, 482.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (165.09, 67.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 621, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3274) [M::mem_pestat] mean and std.dev: (590.74, 511.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 471, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2601) [M::mem_pestat] mean and std.dev: (637.58, 567.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754816 sequences (100000296 bp) in 1.832 CPU sec, 1.835 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 303894, 23, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 213, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (305.29, 209.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.64, 69.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 467, 1680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4458) [M::mem_pestat] mean and std.dev: (906.77, 901.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5847) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 382, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1924) [M::mem_pestat] mean and std.dev: (435.58, 336.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 303937, 21, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 358, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2403) [M::mem_pestat] mean and std.dev: (382.12, 286.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (168.35, 70.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 356, 1711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4853) [M::mem_pestat] mean and std.dev: (730.47, 968.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6424) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 323, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (296.90, 181.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 759646 reads in 367.186 CPU sec, 11.859 real sec [M::mem_process_seqs] Processed 756558 reads in 229.084 CPU sec, 7.439 real sec [M::mem_process_seqs] Processed 756336 reads in 251.376 CPU sec, 8.214 real sec sorting block 0, #reads = 1118834 mem = 536879363 peak = 573132800 [M::process] read 754474 sequences (100000137 bp) in 2.760 CPU sec, 2.926 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 303643, 27, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 219, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1404) [M::mem_pestat] mean and std.dev: (235.52, 154.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (169.33, 70.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 504, 2453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6957) [M::mem_pestat] mean and std.dev: (1276.38, 1728.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 303, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3108) [M::mem_pestat] mean and std.dev: (550.06, 730.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 1, #reads = 1118141 mem = 536881728 peak = 877621248 [M::process] read 753602 sequences (100000222 bp) in 2.485 CPU sec, 2.645 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 303072, 19, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 256, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (264.00, 143.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (169.79, 71.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 220, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (279.18, 204.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 430, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3818) [M::mem_pestat] mean and std.dev: (814.15, 996.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754816 reads in 214.465 CPU sec, 6.894 real sec [M::mem_process_seqs] Processed 754474 reads in 141.028 CPU sec, 4.490 real sec [M::process] read 752974 sequences (100000273 bp) in 2.120 CPU sec, 2.188 real sec... sorting block 2, #reads = 1118753 mem = 536884439 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 303691, 27, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 443, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4024) [M::mem_pestat] mean and std.dev: (732.51, 764.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (170.62, 71.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 695, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4734) [M::mem_pestat] mean and std.dev: (1095.78, 1121.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 316, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (289.42, 160.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751762 sequences (100000025 bp) in 2.731 CPU sec, 3.086 real sec... [M::process] read 752484 sequences (100000046 bp) in 1.884 CPU sec, 1.895 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 303744, 26, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 362, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795) [M::mem_pestat] mean and std.dev: (353.62, 241.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (170.89, 71.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 359, 1320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3716) [M::mem_pestat] mean and std.dev: (710.00, 887.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4914) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 421, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (529.05, 480.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 753602 reads in 211.523 CPU sec, 6.831 real sec [M::mem_process_seqs] Processed 752974 reads in 139.787 CPU sec, 4.532 real sec sorting block 3, #reads = 1116281 mem = 536880116 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 302834, 31, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 527, 1653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4539) [M::mem_pestat] mean and std.dev: (1060.15, 1142.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.18, 71.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (120, 405, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061) [M::mem_pestat] mean and std.dev: (467.41, 481.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 270, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012) [M::mem_pestat] mean and std.dev: (415.05, 376.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751306 sequences (100000266 bp) in 2.409 CPU sec, 2.688 real sec... [M::process] read 750604 sequences (100000044 bp) in 2.131 CPU sec, 2.142 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 303442, 24, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 730, 1518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4240) [M::mem_pestat] mean and std.dev: (822.61, 847.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.42, 72.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 1071, 1644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4348) [M::mem_pestat] mean and std.dev: (893.30, 579.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 371, 1666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4636) [M::mem_pestat] mean and std.dev: (839.68, 912.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 751762 reads in 208.652 CPU sec, 6.707 real sec [M::mem_process_seqs] Processed 752484 reads in 139.272 CPU sec, 4.466 real sec sorting block 4, #reads = 1117479 mem = 536879705 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 302828, 24, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 726, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4299) [M::mem_pestat] mean and std.dev: (915.84, 841.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (172.21, 72.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (468, 686, 1773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4383) [M::mem_pestat] mean and std.dev: (996.27, 975.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5688) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 305, 1981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5617) [M::mem_pestat] mean and std.dev: (695.19, 1021.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 5, #reads = 1117659 mem = 536880086 peak = 877621248 [M::process] read 750392 sequences (100000219 bp) in 3.164 CPU sec, 3.416 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 302324, 30, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 266, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2952) [M::mem_pestat] mean and std.dev: (559.28, 603.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (172.51, 72.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 281, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4145) [M::mem_pestat] mean and std.dev: (656.90, 771.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5520) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 985, 1551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4273) [M::mem_pestat] mean and std.dev: (854.82, 677.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749346 sequences (100000078 bp) in 2.222 CPU sec, 2.233 real sec... [M::mem_process_seqs] Processed 751306 reads in 216.959 CPU sec, 6.973 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 302066, 19, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 386, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1901) [M::mem_pestat] mean and std.dev: (404.62, 278.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (172.50, 72.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 498, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2159) [M::mem_pestat] mean and std.dev: (411.38, 271.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 368, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2990) [M::mem_pestat] mean and std.dev: (567.09, 605.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747900 sequences (100000023 bp) in 2.587 CPU sec, 2.597 real sec... [M::mem_process_seqs] Processed 750392 reads in 118.645 CPU sec, 3.788 real sec sorting block 6, #reads = 1116258 mem = 536879773 peak = 877621248 [M::mem_process_seqs] Processed 750604 reads in 307.513 CPU sec, 9.846 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 301991, 34, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 413, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1915) [M::mem_pestat] mean and std.dev: (496.78, 409.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.36, 72.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (283, 906, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3793) [M::mem_pestat] mean and std.dev: (883.94, 738.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 444, 1103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2947) [M::mem_pestat] mean and std.dev: (640.88, 670.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 7, #reads = 1115915 mem = 536878828 peak = 877621248 [M::process] read 747352 sequences (100000142 bp) in 3.445 CPU sec, 3.731 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 301147, 23, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 346, 1408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3908) [M::mem_pestat] mean and std.dev: (716.50, 827.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5158) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (174.23, 73.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 539, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2438) [M::mem_pestat] mean and std.dev: (487.61, 320.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3122) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 236, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (368.26, 309.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749346 reads in 239.622 CPU sec, 7.695 real sec [M::mem_process_seqs] Processed 747900 reads in 142.750 CPU sec, 4.564 real sec [M::process] read 746766 sequences (100000049 bp) in 2.541 CPU sec, 2.714 real sec... sorting block 8, #reads = 1114665 mem = 536881968 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 301112, 19, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 192, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (294.70, 235.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.67, 73.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (380, 594, 1986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5198) [M::mem_pestat] mean and std.dev: (1017.95, 791.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 485, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2568) [M::mem_pestat] mean and std.dev: (593.56, 511.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3361) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747052 sequences (100000097 bp) in 3.087 CPU sec, 3.359 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 301200, 19, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 265, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (292.89, 208.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.80, 73.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (121, 295, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (491.28, 535.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 222, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738) [M::mem_pestat] mean and std.dev: (405.06, 350.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747352 reads in 218.342 CPU sec, 6.997 real sec [M::process] read 747316 sequences (100000216 bp) in 2.064 CPU sec, 2.084 real sec... [M::mem_process_seqs] Processed 746766 reads in 140.063 CPU sec, 4.459 real sec sorting block 9, #reads = 1113137 mem = 536879434 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 300608, 43, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 263, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1487) [M::mem_pestat] mean and std.dev: (325.32, 241.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.88, 73.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 280, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2813) [M::mem_pestat] mean and std.dev: (478.32, 527.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 370, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4027) [M::mem_pestat] mean and std.dev: (744.81, 723.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5322) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747634 sequences (100000269 bp) in 3.742 CPU sec, 4.053 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 301357, 13, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 182, 1615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4559) [M::mem_pestat] mean and std.dev: (799.41, 1134.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (174.37, 73.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (253, 742, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3361) [M::mem_pestat] mean and std.dev: (668.42, 484.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4397) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 371, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2648) [M::mem_pestat] mean and std.dev: (484.52, 439.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747052 reads in 221.511 CPU sec, 7.097 real sec [M::process] read 747268 sequences (100000088 bp) in 2.351 CPU sec, 2.396 real sec... sorting block 10, #reads = 1112327 mem = 536883200 peak = 877621248 [M::mem_process_seqs] Processed 747316 reads in 145.171 CPU sec, 4.618 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 300886, 35, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 334, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2066) [M::mem_pestat] mean and std.dev: (579.78, 528.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (174.26, 73.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 470, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2461) [M::mem_pestat] mean and std.dev: (483.97, 472.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 645, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2476) [M::mem_pestat] mean and std.dev: (594.84, 507.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 11, #reads = 1112719 mem = 536883326 peak = 877621248 [M::process] read 747520 sequences (100000115 bp) in 3.549 CPU sec, 3.830 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 301080, 21, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 480, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2673) [M::mem_pestat] mean and std.dev: (624.41, 559.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.58, 73.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (434, 772, 2252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5888) [M::mem_pestat] mean and std.dev: (1250.19, 1075.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 291, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2365) [M::mem_pestat] mean and std.dev: (434.41, 368.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747014 sequences (100000149 bp) in 2.035 CPU sec, 2.103 real sec... [M::mem_process_seqs] Processed 747634 reads in 219.221 CPU sec, 7.029 real sec [M::mem_process_seqs] Processed 747268 reads in 148.399 CPU sec, 4.750 real sec sorting block 12, #reads = 1112487 mem = 536882570 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 300566, 26, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 321, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (306.79, 202.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.66, 73.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 1547, 2251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6167) [M::mem_pestat] mean and std.dev: (1482.42, 1385.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8125) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 277, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (250.68, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 748834 sequences (100000069 bp) in 3.558 CPU sec, 3.814 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 301200, 28, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 288, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (287.90, 171.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.37, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (159, 664, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4275) [M::mem_pestat] mean and std.dev: (862.18, 873.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5647) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 387, 1059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2863) [M::mem_pestat] mean and std.dev: (474.59, 555.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747520 reads in 220.763 CPU sec, 7.099 real sec [M::process] read 757244 sequences (100000051 bp) in 2.214 CPU sec, 2.273 real sec... [M::mem_process_seqs] Processed 747014 reads in 145.384 CPU sec, 4.683 real sec sorting block 13, #reads = 1110788 mem = 536878424 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 301102, 30, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 175, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299) [M::mem_pestat] mean and std.dev: (213.00, 158.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (173.92, 73.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 405, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1910) [M::mem_pestat] mean and std.dev: (502.23, 500.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 217, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (285.78, 273.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 756996 sequences (100000134 bp) in 3.417 CPU sec, 3.711 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 304251, 28, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 244, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2947) [M::mem_pestat] mean and std.dev: (678.80, 773.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.11, 69.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 611, 3311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9619) [M::mem_pestat] mean and std.dev: (1607.86, 2072.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 593, 2163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6055) [M::mem_pestat] mean and std.dev: (1115.58, 1416.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755862 sequences (100000192 bp) in 2.139 CPU sec, 2.144 real sec... [M::mem_process_seqs] Processed 748834 reads in 223.346 CPU sec, 7.180 real sec sorting block 14, #reads = 1109112 mem = 536878128 peak = 877621248 [M::mem_process_seqs] Processed 757244 reads in 144.946 CPU sec, 4.647 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 304675, 14, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 238, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2720) [M::mem_pestat] mean and std.dev: (551.36, 575.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (167.77, 70.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 995, 1438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4024) [M::mem_pestat] mean and std.dev: (949.14, 942.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5317) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 347, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2008) [M::mem_pestat] mean and std.dev: (327.43, 204.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 15, #reads = 1112173 mem = 536876891 peak = 877621248 [M::process] read 755192 sequences (100000103 bp) in 3.669 CPU sec, 3.937 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 304497, 18, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 599, 1985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5449) [M::mem_pestat] mean and std.dev: (1024.85, 1033.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (168.52, 70.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 623, 2557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7169) [M::mem_pestat] mean and std.dev: (1276.67, 1459.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 272, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (263.64, 173.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 756996 reads in 211.038 CPU sec, 6.776 real sec [M::mem_process_seqs] Processed 755862 reads in 138.321 CPU sec, 4.397 real sec [M::process] read 755568 sequences (100000013 bp) in 2.409 CPU sec, 2.534 real sec... sorting block 16, #reads = 1121735 mem = 536878613 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304171, 22, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 690, 1826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5160) [M::mem_pestat] mean and std.dev: (1278.90, 1392.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (168.99, 70.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 281, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112) [M::mem_pestat] mean and std.dev: (461.84, 469.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 277, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (300.97, 227.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754030 sequences (100000179 bp) in 3.870 CPU sec, 4.250 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 304013, 23, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 293, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (237.76, 91.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (169.02, 71.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 776, 2031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5737) [M::mem_pestat] mean and std.dev: (1185.86, 1277.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 259, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (484.91, 514.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2544) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755192 reads in 212.028 CPU sec, 6.865 real sec [M::mem_process_seqs] Processed 755568 reads in 144.733 CPU sec, 4.635 real sec sorting block 17, #reads = 1119940 mem = 536881918 peak = 877621248 [M::process] read 753748 sequences (100000272 bp) in 3.059 CPU sec, 3.259 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 303903, 19, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 321, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831) [M::mem_pestat] mean and std.dev: (448.31, 368.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (170.04, 71.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (181, 296, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1501) [M::mem_pestat] mean and std.dev: (402.83, 331.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 340, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2763) [M::mem_pestat] mean and std.dev: (637.25, 634.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 752368 sequences (100000024 bp) in 3.327 CPU sec, 3.732 real sec... sorting block 18, #reads = 1119934 mem = 536888083 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 303600, 18, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 430, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (662.94, 654.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (170.62, 72.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (161, 475, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4250) [M::mem_pestat] mean and std.dev: (950.33, 1155.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 853, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2300) [M::mem_pestat] mean and std.dev: (634.16, 469.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754030 reads in 212.407 CPU sec, 6.847 real sec [M::mem_process_seqs] Processed 753748 reads in 142.950 CPU sec, 4.583 real sec [M::process] read 751966 sequences (100000231 bp) in 2.992 CPU sec, 3.191 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 302821, 18, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 370, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2465) [M::mem_pestat] mean and std.dev: (491.91, 351.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.34, 72.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 721, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2110) [M::mem_pestat] mean and std.dev: (629.20, 409.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 196, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (196.25, 73.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 19, #reads = 1117968 mem = 536881757 peak = 877621248 [M::process] read 751430 sequences (100000085 bp) in 3.787 CPU sec, 4.149 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 303129, 23, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 276, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172) [M::mem_pestat] mean and std.dev: (360.30, 286.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.64, 72.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (336, 1260, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3597) [M::mem_pestat] mean and std.dev: (820.85, 657.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 383, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3854) [M::mem_pestat] mean and std.dev: (741.42, 772.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 752368 reads in 208.947 CPU sec, 6.792 real sec [M::mem_process_seqs] Processed 751966 reads in 139.625 CPU sec, 4.512 real sec sorting block 20, #reads = 1117480 mem = 536876041 peak = 877621248 [M::process] read 751056 sequences (100000083 bp) in 3.297 CPU sec, 3.573 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 302630, 26, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 277, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2008) [M::mem_pestat] mean and std.dev: (365.45, 369.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.94, 72.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 286, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2575) [M::mem_pestat] mean and std.dev: (479.00, 562.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 235, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (242.22, 140.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750108 sequences (100000108 bp) in 3.039 CPU sec, 3.344 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 302522, 24, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 317, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153) [M::mem_pestat] mean and std.dev: (418.66, 441.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (172.04, 72.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (175, 360, 2087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5911) [M::mem_pestat] mean and std.dev: (942.42, 1052.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 226, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1387) [M::mem_pestat] mean and std.dev: (362.11, 233.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 751430 reads in 211.705 CPU sec, 6.839 real sec [M::mem_process_seqs] Processed 751056 reads in 140.519 CPU sec, 4.510 real sec sorting block 21, #reads = 1116854 mem = 536883952 peak = 877621248 [M::process] read 749096 sequences (100000056 bp) in 3.045 CPU sec, 3.217 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 302833, 23, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 425, 1549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4283) [M::mem_pestat] mean and std.dev: (809.32, 907.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (173.36, 73.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (348, 601, 2098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5598) [M::mem_pestat] mean and std.dev: (899.52, 864.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 298, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2268) [M::mem_pestat] mean and std.dev: (505.11, 476.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747218 sequences (100000198 bp) in 3.125 CPU sec, 3.411 real sec... sorting block 22, #reads = 1115006 mem = 536882361 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 301641, 14, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 278, 1352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3760) [M::mem_pestat] mean and std.dev: (792.15, 902.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (173.70, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 458, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2046) [M::mem_pestat] mean and std.dev: (452.31, 399.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 305, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1822) [M::mem_pestat] mean and std.dev: (457.81, 423.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 750108 reads in 210.206 CPU sec, 6.770 real sec [M::mem_process_seqs] Processed 749096 reads in 139.050 CPU sec, 4.438 real sec [M::process] read 747726 sequences (100000052 bp) in 2.607 CPU sec, 2.713 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 300883, 29, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 205, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744) [M::mem_pestat] mean and std.dev: (349.34, 388.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.17, 73.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (324, 431, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2364) [M::mem_pestat] mean and std.dev: (587.79, 461.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 338, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2670) [M::mem_pestat] mean and std.dev: (532.50, 480.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 23, #reads = 1116064 mem = 536879347 peak = 877621248 [M::process] read 746646 sequences (100000120 bp) in 2.686 CPU sec, 2.928 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 300925, 19, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 444, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2395) [M::mem_pestat] mean and std.dev: (485.96, 400.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.48, 73.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 360, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3487) [M::mem_pestat] mean and std.dev: (632.33, 672.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4599) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 431, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2590) [M::mem_pestat] mean and std.dev: (554.36, 451.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747218 reads in 200.970 CPU sec, 6.466 real sec [M::process] read 747538 sequences (100000057 bp) in 2.117 CPU sec, 2.139 real sec... [M::mem_process_seqs] Processed 747726 reads in 135.713 CPU sec, 4.341 real sec sorting block 24, #reads = 1113017 mem = 536880099 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 300615, 21, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 463, 1486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4104) [M::mem_pestat] mean and std.dev: (696.17, 765.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.33, 73.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 570, 2059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5961) [M::mem_pestat] mean and std.dev: (1126.57, 1392.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 380, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3648) [M::mem_pestat] mean and std.dev: (823.86, 809.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747500 sequences (100000118 bp) in 2.991 CPU sec, 3.261 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 301163, 44, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 452, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1994) [M::mem_pestat] mean and std.dev: (488.13, 403.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (174.11, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 718, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2875) [M::mem_pestat] mean and std.dev: (844.40, 649.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (279, 843, 991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2415) [M::mem_pestat] mean and std.dev: (680.47, 500.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 746646 reads in 200.014 CPU sec, 6.430 real sec [M::process] read 747424 sequences (100000203 bp) in 2.270 CPU sec, 2.304 real sec... sorting block 25, #reads = 1112643 mem = 536877217 peak = 877621248 [M::mem_process_seqs] Processed 747538 reads in 135.865 CPU sec, 4.351 real sec sorting block 26, #reads = 1112491 mem = 536879061 peak = 877621248 [M::process] read 748248 sequences (100000257 bp) in 3.137 CPU sec, 3.410 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 301534, 24, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 310, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2660) [M::mem_pestat] mean and std.dev: (617.81, 668.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.69, 73.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 656, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4452) [M::mem_pestat] mean and std.dev: (745.18, 821.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5874) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 444, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2975) [M::mem_pestat] mean and std.dev: (692.32, 706.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747424 reads in 113.713 CPU sec, 3.650 real sec [M::process] read 748044 sequences (100000020 bp) in 2.315 CPU sec, 2.411 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 301535, 31, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 214, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1605) [M::mem_pestat] mean and std.dev: (315.41, 255.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (174.28, 73.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 356, 1328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3614) [M::mem_pestat] mean and std.dev: (756.00, 949.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4757) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 366, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3063) [M::mem_pestat] mean and std.dev: (496.59, 403.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 301401, 20, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 241, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (341.53, 276.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (174.16, 73.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 893, 1587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4293) [M::mem_pestat] mean and std.dev: (1049.32, 1105.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 291, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146) [M::mem_pestat] mean and std.dev: (508.87, 493.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747500 reads in 312.132 CPU sec, 10.104 real sec [M::process] read 748580 sequences (100000100 bp) in 2.906 CPU sec, 3.071 real sec... [M::mem_process_seqs] Processed 748248 reads in 159.438 CPU sec, 5.202 real sec sorting block 27, #reads = 1113237 mem = 536878738 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 300989, 29, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (284.27, 209.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (174.31, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 485, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3088) [M::mem_pestat] mean and std.dev: (695.11, 758.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 192, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (313.84, 255.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 28, #reads = 1112786 mem = 536881440 peak = 877621248 [M::process] read 750930 sequences (100000261 bp) in 3.581 CPU sec, 3.940 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 301022, 36, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 403, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2546) [M::mem_pestat] mean and std.dev: (558.61, 542.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (174.03, 73.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (111, 428, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2676) [M::mem_pestat] mean and std.dev: (565.00, 522.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 315, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828) [M::mem_pestat] mean and std.dev: (446.12, 427.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 748044 reads in 208.137 CPU sec, 6.748 real sec [M::mem_process_seqs] Processed 748580 reads in 142.373 CPU sec, 4.584 real sec [M::process] read 758638 sequences (100000081 bp) in 2.379 CPU sec, 2.478 real sec... sorting block 29, #reads = 1111299 mem = 536878795 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 301751, 28, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 286, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761) [M::mem_pestat] mean and std.dev: (401.21, 283.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (172.43, 72.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 327, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061) [M::mem_pestat] mean and std.dev: (449.52, 500.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 299, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2691) [M::mem_pestat] mean and std.dev: (451.09, 521.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 758032 sequences (100000090 bp) in 3.147 CPU sec, 3.411 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 305167, 26, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 270, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2596) [M::mem_pestat] mean and std.dev: (477.89, 527.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 154, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (166.51, 69.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 455, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3163) [M::mem_pestat] mean and std.dev: (778.33, 879.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 395, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3382) [M::mem_pestat] mean and std.dev: (804.35, 863.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 750930 reads in 209.602 CPU sec, 6.745 real sec [M::process] read 757770 sequences (100000211 bp) in 2.171 CPU sec, 2.189 real sec... [M::mem_process_seqs] Processed 758638 reads in 136.384 CPU sec, 4.361 real sec sorting block 30, #reads = 1108872 mem = 536882887 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 305038, 21, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 523, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2301) [M::mem_pestat] mean and std.dev: (550.18, 465.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 154, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (166.48, 68.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 889, 3008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8688) [M::mem_pestat] mean and std.dev: (1871.76, 2436.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 396, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2140) [M::mem_pestat] mean and std.dev: (491.80, 463.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2812) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 757202 sequences (100000176 bp) in 3.647 CPU sec, 4.008 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 304952, 20, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 252, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193) [M::mem_pestat] mean and std.dev: (438.12, 381.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 154, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (166.72, 69.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 818, 2034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5686) [M::mem_pestat] mean and std.dev: (965.95, 937.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 403, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3137) [M::mem_pestat] mean and std.dev: (645.24, 504.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 758032 reads in 211.290 CPU sec, 6.827 real sec sorting block 31, #reads = 1120048 mem = 536879933 peak = 877621248 [M::mem_process_seqs] Processed 757770 reads in 140.205 CPU sec, 4.489 real sec [M::process] read 756054 sequences (100000146 bp) in 3.200 CPU sec, 3.378 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 304570, 15, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 290, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (329.29, 198.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.24, 69.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 505, 1347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3419) [M::mem_pestat] mean and std.dev: (788.93, 771.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 376, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739) [M::mem_pestat] mean and std.dev: (519.57, 382.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 32, #reads = 1120323 mem = 536876760 peak = 877621248 [M::process] read 755206 sequences (100000205 bp) in 3.012 CPU sec, 3.403 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 304694, 27, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 273, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2574) [M::mem_pestat] mean and std.dev: (416.50, 373.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.47, 69.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 307, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2903) [M::mem_pestat] mean and std.dev: (527.54, 617.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 339, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3707) [M::mem_pestat] mean and std.dev: (705.12, 729.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 757202 reads in 208.323 CPU sec, 6.776 real sec [M::mem_process_seqs] Processed 756054 reads in 141.529 CPU sec, 4.537 real sec [M::process] read 755554 sequences (100000004 bp) in 2.646 CPU sec, 2.779 real sec... sorting block 33, #reads = 1120563 mem = 536879258 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 303724, 24, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 183, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2052) [M::mem_pestat] mean and std.dev: (410.51, 426.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (168.68, 70.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 506, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2824) [M::mem_pestat] mean and std.dev: (642.41, 635.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3687) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 294, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1998) [M::mem_pestat] mean and std.dev: (419.32, 355.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754008 sequences (100000127 bp) in 3.406 CPU sec, 3.786 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 304377, 28, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 235, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1638) [M::mem_pestat] mean and std.dev: (361.58, 259.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (168.63, 70.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 110, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (342.50, 447.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 215, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (220.19, 103.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755206 reads in 206.023 CPU sec, 6.642 real sec [M::mem_process_seqs] Processed 755554 reads in 140.215 CPU sec, 4.553 real sec [M::process] read 753104 sequences (100000079 bp) in 2.958 CPU sec, 3.195 real sec... sorting block 34, #reads = 1118331 mem = 536878132 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 303825, 20, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 454, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2536) [M::mem_pestat] mean and std.dev: (447.43, 372.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (169.82, 71.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 645, 1680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4728) [M::mem_pestat] mean and std.dev: (862.15, 991.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 471, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2547) [M::mem_pestat] mean and std.dev: (620.58, 551.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 752922 sequences (100000096 bp) in 2.912 CPU sec, 3.196 real sec... sorting block 35, #reads = 1119468 mem = 536878261 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 302962, 23, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 296, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1563) [M::mem_pestat] mean and std.dev: (328.12, 231.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (170.60, 71.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 698, 2096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6008) [M::mem_pestat] mean and std.dev: (669.11, 715.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7964) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 374, 1572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4310) [M::mem_pestat] mean and std.dev: (581.50, 555.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754008 reads in 207.436 CPU sec, 6.726 real sec [M::mem_process_seqs] Processed 753104 reads in 139.316 CPU sec, 4.484 real sec [M::process] read 753142 sequences (100000093 bp) in 2.627 CPU sec, 2.791 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 303480, 14, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 604, 1884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5186) [M::mem_pestat] mean and std.dev: (862.28, 993.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (170.88, 72.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 858, 1955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5467) [M::mem_pestat] mean and std.dev: (1032.54, 1060.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 245, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1754) [M::mem_pestat] mean and std.dev: (363.77, 265.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 36, #reads = 1118352 mem = 536877377 peak = 877621248 [M::process] read 751920 sequences (100000294 bp) in 3.552 CPU sec, 3.935 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 303476, 22, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 517, 1358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3764) [M::mem_pestat] mean and std.dev: (734.82, 763.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (170.37, 71.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 509, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1856) [M::mem_pestat] mean and std.dev: (377.32, 318.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3762) [M::mem_pestat] mean and std.dev: (674.24, 930.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 752922 reads in 207.120 CPU sec, 6.774 real sec [M::mem_process_seqs] Processed 753142 reads in 139.021 CPU sec, 4.565 real sec [M::process] read 751470 sequences (100000163 bp) in 2.684 CPU sec, 2.900 real sec... sorting block 37, #reads = 1117477 mem = 536880833 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 302875, 26, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 267, 1867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5277) [M::mem_pestat] mean and std.dev: (996.91, 1246.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.62, 72.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 526, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3034) [M::mem_pestat] mean and std.dev: (549.64, 591.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4002) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 522, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3714) [M::mem_pestat] mean and std.dev: (762.56, 711.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749950 sequences (100000111 bp) in 3.080 CPU sec, 3.397 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 302972, 27, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 372, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3800) [M::mem_pestat] mean and std.dev: (710.64, 664.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.75, 72.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (403, 557, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3211) [M::mem_pestat] mean and std.dev: (799.19, 672.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4147) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 274, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (331.57, 227.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 751920 reads in 208.310 CPU sec, 6.752 real sec sorting block 38, #reads = 1116626 mem = 536880099 peak = 877621248 [M::process] read 750202 sequences (100000099 bp) in 2.453 CPU sec, 2.583 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 302254, 31, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 477, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2526) [M::mem_pestat] mean and std.dev: (662.48, 571.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.38, 72.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (139, 476, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368) [M::mem_pestat] mean and std.dev: (481.59, 475.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 417, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2197) [M::mem_pestat] mean and std.dev: (509.86, 429.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749950 reads in 118.459 CPU sec, 3.795 real sec [M::process] read 749272 sequences (100000172 bp) in 2.493 CPU sec, 2.519 real sec... sorting block 39, #reads = 1114814 mem = 536880655 peak = 877621248 [M::mem_process_seqs] Processed 751470 reads in 310.935 CPU sec, 10.032 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 301867, 23, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 276, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757) [M::mem_pestat] mean and std.dev: (327.17, 323.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.35, 72.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (381, 544, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1986) [M::mem_pestat] mean and std.dev: (681.17, 485.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 257, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (420.69, 320.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 748838 sequences (100000141 bp) in 3.083 CPU sec, 3.390 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 302459, 21, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 200, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480) [M::mem_pestat] mean and std.dev: (317.46, 268.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (173.71, 73.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 422, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2969) [M::mem_pestat] mean and std.dev: (551.15, 499.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 305, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (248.23, 101.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 750202 reads in 220.226 CPU sec, 7.095 real sec [M::mem_process_seqs] Processed 749272 reads in 134.644 CPU sec, 4.325 real sec sorting block 40, #reads = 1116100 mem = 536881052 peak = 877621248 [M::process] read 748282 sequences (100000046 bp) in 3.187 CPU sec, 3.386 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 301603, 32, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 224, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (257.25, 139.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (173.76, 73.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 674, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2880) [M::mem_pestat] mean and std.dev: (648.63, 521.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 557, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3835) [M::mem_pestat] mean and std.dev: (679.03, 688.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 41, #reads = 1115166 mem = 536878734 peak = 877621248 [M::process] read 748504 sequences (100000157 bp) in 2.868 CPU sec, 3.237 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 301853, 27, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 409, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2271) [M::mem_pestat] mean and std.dev: (525.68, 508.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (173.92, 73.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 710, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3541) [M::mem_pestat] mean and std.dev: (818.73, 843.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 230, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1604) [M::mem_pestat] mean and std.dev: (307.07, 289.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 748838 reads in 199.506 CPU sec, 6.424 real sec [M::mem_process_seqs] Processed 748282 reads in 134.355 CPU sec, 4.303 real sec [M::process] read 748470 sequences (100000035 bp) in 2.413 CPU sec, 2.546 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 301870, 39, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 338, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3497) [M::mem_pestat] mean and std.dev: (617.37, 551.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (173.82, 73.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (207, 391, 854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2148) [M::mem_pestat] mean and std.dev: (496.17, 471.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 244, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1413) [M::mem_pestat] mean and std.dev: (329.13, 289.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 42, #reads = 1113501 mem = 536880273 peak = 877621248 [M::process] read 749230 sequences (100000250 bp) in 3.358 CPU sec, 3.616 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 301605, 46, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 292, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1393) [M::mem_pestat] mean and std.dev: (287.39, 192.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (173.98, 73.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 709, 1825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4825) [M::mem_pestat] mean and std.dev: (1256.96, 1218.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 414, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2568) [M::mem_pestat] mean and std.dev: (540.64, 410.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 748504 reads in 199.962 CPU sec, 6.439 real sec [M::mem_process_seqs] Processed 748470 reads in 137.509 CPU sec, 4.380 real sec [M::process] read 748604 sequences (100000051 bp) in 2.800 CPU sec, 2.955 real sec... sorting block 43, #reads = 1113695 mem = 536877920 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 301658, 22, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 375, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3496) [M::mem_pestat] mean and std.dev: (716.77, 868.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.21, 72.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 601, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2428) [M::mem_pestat] mean and std.dev: (525.28, 428.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 192, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1778) [M::mem_pestat] mean and std.dev: (359.29, 373.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2320) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 752932 sequences (100000238 bp) in 3.137 CPU sec, 3.414 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 301631, 26, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 253, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (246.44, 128.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (173.98, 73.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 550, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3529) [M::mem_pestat] mean and std.dev: (760.12, 759.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 276, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2355) [M::mem_pestat] mean and std.dev: (540.70, 538.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749230 reads in 205.050 CPU sec, 6.574 real sec [M::process] read 758392 sequences (100000094 bp) in 2.290 CPU sec, 2.350 real sec... sorting block 44, #reads = 1113145 mem = 536884713 peak = 877621248 [M::mem_process_seqs] Processed 748604 reads in 138.317 CPU sec, 4.414 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 302742, 23, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 733, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5329) [M::mem_pestat] mean and std.dev: (931.41, 1106.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (170.50, 71.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 513, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3285) [M::mem_pestat] mean and std.dev: (773.04, 814.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 240, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (274.96, 176.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 45, #reads = 1110865 mem = 536880436 peak = 877621248 [M::process] read 757868 sequences (100000218 bp) in 3.243 CPU sec, 3.815 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 304319, 27, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 204, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1717) [M::mem_pestat] mean and std.dev: (275.35, 247.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 154, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (166.35, 68.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 504, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329) [M::mem_pestat] mean and std.dev: (612.52, 504.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 427, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2495) [M::mem_pestat] mean and std.dev: (671.00, 540.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 752932 reads in 200.085 CPU sec, 6.462 real sec [M::mem_process_seqs] Processed 758392 reads in 132.639 CPU sec, 4.272 real sec [M::process] read 756706 sequences (100000191 bp) in 2.478 CPU sec, 2.606 real sec... sorting block 46, #reads = 1113203 mem = 536882615 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 305148, 21, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 239, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2418) [M::mem_pestat] mean and std.dev: (578.62, 578.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 154, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (166.75, 69.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (110, 259, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1751) [M::mem_pestat] mean and std.dev: (310.32, 265.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 185, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2249) [M::mem_pestat] mean and std.dev: (430.79, 445.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2946) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 756140 sequences (100000125 bp) in 2.988 CPU sec, 3.324 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 304792, 13, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 296, 1540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4270) [M::mem_pestat] mean and std.dev: (653.29, 766.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (167.56, 69.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 490, 991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2693) [M::mem_pestat] mean and std.dev: (597.77, 546.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 263, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1982) [M::mem_pestat] mean and std.dev: (446.82, 417.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 757868 reads in 196.285 CPU sec, 6.328 real sec [M::process] read 756932 sequences (100000089 bp) in 2.173 CPU sec, 2.211 real sec... [M::mem_process_seqs] Processed 756706 reads in 132.130 CPU sec, 4.200 real sec sorting block 47, #reads = 1121658 mem = 536884585 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 304398, 22, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 252, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1145) [M::mem_pestat] mean and std.dev: (249.33, 148.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (167.90, 69.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 558, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3861) [M::mem_pestat] mean and std.dev: (792.14, 685.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 309, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2575) [M::mem_pestat] mean and std.dev: (470.13, 409.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755374 sequences (100000071 bp) in 2.762 CPU sec, 3.060 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 304826, 25, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 155, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (223.50, 154.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (167.04, 69.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (173, 520, 1679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4691) [M::mem_pestat] mean and std.dev: (933.33, 961.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 295, 1594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4508) [M::mem_pestat] mean and std.dev: (499.33, 784.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 756140 reads in 195.917 CPU sec, 6.284 real sec [M::process] read 755172 sequences (100000181 bp) in 2.221 CPU sec, 2.242 real sec... sorting block 48, #reads = 1121501 mem = 536878493 peak = 877621248 [M::mem_process_seqs] Processed 756932 reads in 133.304 CPU sec, 4.242 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304263, 21, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 208, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2037) [M::mem_pestat] mean and std.dev: (455.60, 565.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (168.31, 70.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 446, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2928) [M::mem_pestat] mean and std.dev: (643.50, 591.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 379, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2833) [M::mem_pestat] mean and std.dev: (656.58, 641.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 49, #reads = 1119496 mem = 536877996 peak = 877621248 [M::process] read 754758 sequences (100000064 bp) in 3.383 CPU sec, 3.763 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 303787, 19, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 207, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (280.03, 223.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (168.84, 70.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 84, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1814) [M::mem_pestat] mean and std.dev: (331.72, 383.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2393) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 249, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2870) [M::mem_pestat] mean and std.dev: (507.36, 496.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755374 reads in 191.871 CPU sec, 6.187 real sec [M::mem_process_seqs] Processed 755172 reads in 129.928 CPU sec, 4.154 real sec [M::process] read 754186 sequences (100000158 bp) in 2.582 CPU sec, 2.722 real sec... sorting block 50, #reads = 1119607 mem = 536877293 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 304128, 35, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 313, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2102) [M::mem_pestat] mean and std.dev: (395.83, 399.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.25, 70.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 707, 1783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4729) [M::mem_pestat] mean and std.dev: (1139.71, 1119.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 342, 1059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2791) [M::mem_pestat] mean and std.dev: (627.94, 490.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3657) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 303424, 25, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 216, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1398) [M::mem_pestat] mean and std.dev: (290.23, 259.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.32, 70.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 137, 2430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7176) [M::mem_pestat] mean and std.dev: (630.09, 1121.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9549) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 338, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940) [M::mem_pestat] mean and std.dev: (518.43, 499.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754090 sequences (100000152 bp) in 3.931 CPU sec, 4.407 real sec... [M::mem_process_seqs] Processed 754758 reads in 194.531 CPU sec, 6.311 real sec [M::mem_process_seqs] Processed 754186 reads in 130.288 CPU sec, 4.197 real sec sorting block 51, #reads = 1119331 mem = 536880827 peak = 877621248 [M::process] read 753862 sequences (100000087 bp) in 3.063 CPU sec, 3.347 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 303556, 30, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 256, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3214) [M::mem_pestat] mean and std.dev: (586.69, 606.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.78, 71.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 741, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1969) [M::mem_pestat] mean and std.dev: (494.56, 342.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 255, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (243.50, 79.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 303189, 17, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681) [M::mem_pestat] mean and std.dev: (415.18, 379.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (169.94, 71.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 524, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4582) [M::mem_pestat] mean and std.dev: (718.31, 715.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 255, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432) [M::mem_pestat] mean and std.dev: (349.07, 270.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 753016 sequences (100000149 bp) in 3.189 CPU sec, 3.561 real sec... sorting block 52, #reads = 1118799 mem = 536878008 peak = 877621248 [M::mem_process_seqs] Processed 754090 reads in 197.216 CPU sec, 6.385 real sec [M::mem_process_seqs] Processed 753862 reads in 132.472 CPU sec, 4.261 real sec [M::process] read 751904 sequences (100000044 bp) in 2.868 CPU sec, 3.196 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 302988, 26, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 340, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013) [M::mem_pestat] mean and std.dev: (367.15, 280.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (170.70, 71.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (85, 673, 1552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4486) [M::mem_pestat] mean and std.dev: (855.31, 982.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 433, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2594) [M::mem_pestat] mean and std.dev: (501.29, 514.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 53, #reads = 1118599 mem = 536881987 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 302858, 22, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 309, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (411.39, 386.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.68, 72.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (84, 973, 1809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5259) [M::mem_pestat] mean and std.dev: (919.83, 1043.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6984) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 258, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (313.35, 192.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 753016 reads in 198.642 CPU sec, 6.435 real sec [M::process] read 751218 sequences (100000167 bp) in 3.620 CPU sec, 3.954 real sec... [M::mem_process_seqs] Processed 751904 reads in 134.963 CPU sec, 4.338 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 302791, 18, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 341, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2667) [M::mem_pestat] mean and std.dev: (439.66, 459.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (172.40, 72.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (982, 1842, 7208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19660) [M::mem_pestat] mean and std.dev: (3511.94, 3237.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 225, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718) [M::mem_pestat] mean and std.dev: (386.58, 368.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 54, #reads = 1117609 mem = 536882981 peak = 877621248 [M::process] read 750194 sequences (100000155 bp) in 3.202 CPU sec, 3.474 real sec... [M::mem_process_seqs] Processed 751218 reads in 109.186 CPU sec, 3.662 real sec [M::process] read 750054 sequences (100000172 bp) in 2.889 CPU sec, 3.131 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 302397, 20, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 187, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (176.37, 56.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (173.02, 73.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (370, 766, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2737) [M::mem_pestat] mean and std.dev: (693.11, 488.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 314, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4082) [M::mem_pestat] mean and std.dev: (480.10, 549.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 55, #reads = 1117055 mem = 536884805 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 301882, 27, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 297, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2500) [M::mem_pestat] mean and std.dev: (518.79, 653.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (173.54, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (183, 322, 1314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3576) [M::mem_pestat] mean and std.dev: (700.35, 682.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 211, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344) [M::mem_pestat] mean and std.dev: (323.44, 308.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749134 sequences (100000107 bp) in 3.346 CPU sec, 3.635 real sec... [M::mem_process_seqs] Processed 750194 reads in 198.688 CPU sec, 6.416 real sec [M::mem_process_seqs] Processed 750054 reads in 135.343 CPU sec, 4.356 real sec [M::process] read 747920 sequences (100000141 bp) in 3.112 CPU sec, 3.384 real sec... sorting block 56, #reads = 1116075 mem = 536882324 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 302036, 19, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 247, 1722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4876) [M::mem_pestat] mean and std.dev: (807.70, 1123.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (174.31, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (294, 495, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2073) [M::mem_pestat] mean and std.dev: (604.47, 490.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 273, 1311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3525) [M::mem_pestat] mean and std.dev: (758.10, 875.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4632) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749134 reads in 109.537 CPU sec, 3.675 real sec [M::process] read 747692 sequences (100000247 bp) in 3.054 CPU sec, 3.380 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 301460, 26, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 464, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533) [M::mem_pestat] mean and std.dev: (531.56, 480.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (174.98, 74.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (173, 431, 2787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8015) [M::mem_pestat] mean and std.dev: (1328.65, 1602.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 233, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (223.07, 77.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 57, #reads = 1115437 mem = 536879293 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 300806, 28, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 327, 1147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3005) [M::mem_pestat] mean and std.dev: (614.87, 516.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.15, 74.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 428, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4849) [M::mem_pestat] mean and std.dev: (1080.30, 1397.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 215, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (308.25, 231.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747920 reads in 200.442 CPU sec, 6.537 real sec [M::process] read 747270 sequences (100000048 bp) in 3.496 CPU sec, 3.876 real sec... [M::mem_process_seqs] Processed 747692 reads in 134.552 CPU sec, 4.349 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 301172, 22, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 471, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3588) [M::mem_pestat] mean and std.dev: (604.19, 566.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (175.02, 73.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (95, 863, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4559) [M::mem_pestat] mean and std.dev: (845.29, 709.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 442, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3553) [M::mem_pestat] mean and std.dev: (883.97, 956.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 58, #reads = 1114680 mem = 536878659 peak = 877621248 [M::process] read 746234 sequences (100000124 bp) in 3.355 CPU sec, 3.672 real sec... [M::mem_process_seqs] Processed 747270 reads in 110.550 CPU sec, 3.736 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 300543, 18, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 304, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2626) [M::mem_pestat] mean and std.dev: (657.47, 740.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.73, 74.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 672, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3175) [M::mem_pestat] mean and std.dev: (786.78, 783.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 244, 991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2695) [M::mem_pestat] mean and std.dev: (489.26, 598.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 746572 sequences (100000056 bp) in 2.974 CPU sec, 3.292 real sec... [M::mem_process_seqs] Processed 746234 reads in 113.678 CPU sec, 3.842 real sec sorting block 59, #reads = 1113234 mem = 536880432 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 300485, 27, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 412, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4213) [M::mem_pestat] mean and std.dev: (820.11, 842.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.62, 74.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (197, 359, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2657) [M::mem_pestat] mean and std.dev: (463.79, 438.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 506, 2100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5798) [M::mem_pestat] mean and std.dev: (1349.92, 1700.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750878 sequences (100000117 bp) in 3.161 CPU sec, 3.457 real sec... sorting block 60, #reads = 1111592 mem = 536879822 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 301967, 19, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 322, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4008) [M::mem_pestat] mean and std.dev: (790.91, 944.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (172.01, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 962, 2132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5876) [M::mem_pestat] mean and std.dev: (1200.67, 1060.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 366, 1720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4782) [M::mem_pestat] mean and std.dev: (992.12, 1068.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 761826 sequences (100000193 bp) in 3.231 CPU sec, 3.483 real sec... [M::mem_process_seqs] Processed 750878 reads in 120.878 CPU sec, 4.020 real sec [M::mem_process_seqs] Processed 746572 reads in 325.453 CPU sec, 10.801 real sec [M::process] read 760692 sequences (100000200 bp) in 3.122 CPU sec, 3.436 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 305875, 21, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 220, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (257.92, 135.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (162.36, 65.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 570, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3512) [M::mem_pestat] mean and std.dev: (692.63, 519.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4554) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 259, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2795) [M::mem_pestat] mean and std.dev: (606.03, 680.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 61, #reads = 1108642 mem = 536879533 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 305738, 34, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779) [M::mem_pestat] mean and std.dev: (313.22, 299.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 152, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (163.07, 65.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 335, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3372) [M::mem_pestat] mean and std.dev: (677.59, 764.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 397, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175) [M::mem_pestat] mean and std.dev: (492.58, 403.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 761826 reads in 213.472 CPU sec, 6.852 real sec [M::process] read 760222 sequences (100000216 bp) in 3.721 CPU sec, 4.070 real sec... [M::mem_process_seqs] Processed 760692 reads in 142.590 CPU sec, 4.662 real sec sorting block 62, #reads = 1111508 mem = 536879256 peak = 877621248 [M::process] read 757910 sequences (100000153 bp) in 3.296 CPU sec, 3.600 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 305697, 26, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 246, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375) [M::mem_pestat] mean and std.dev: (442.81, 510.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 152, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (163.22, 65.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 1333, 2946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8398) [M::mem_pestat] mean and std.dev: (1858.15, 2020.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11124) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 325, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2121) [M::mem_pestat] mean and std.dev: (495.79, 539.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 304826, 20, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 349, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3886) [M::mem_pestat] mean and std.dev: (648.81, 604.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (165.24, 67.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 715, 1340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3946) [M::mem_pestat] mean and std.dev: (711.17, 773.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 382, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2078) [M::mem_pestat] mean and std.dev: (485.26, 446.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 757134 sequences (100000038 bp) in 2.997 CPU sec, 3.298 real sec... [M::mem_process_seqs] Processed 760222 reads in 211.295 CPU sec, 6.914 real sec [M::mem_process_seqs] Processed 757910 reads in 137.883 CPU sec, 4.492 real sec sorting block 63, #reads = 1119590 mem = 536885919 peak = 877621248 [M::process] read 756710 sequences (100000067 bp) in 3.227 CPU sec, 3.637 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 304421, 23, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 291, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (384.24, 333.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (165.88, 67.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (197, 526, 1513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4145) [M::mem_pestat] mean and std.dev: (1060.48, 1112.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 270, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (257.41, 113.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 757134 reads in 115.766 CPU sec, 3.919 real sec sorting block 64, #reads = 1118637 mem = 536877786 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 304867, 22, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 253, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769) [M::mem_pestat] mean and std.dev: (359.89, 290.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (166.17, 67.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 380, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2663) [M::mem_pestat] mean and std.dev: (433.05, 392.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 298, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (473.76, 465.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755892 sequences (100000203 bp) in 3.232 CPU sec, 3.478 real sec... [M::mem_process_seqs] Processed 756710 reads in 110.996 CPU sec, 3.836 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 304095, 21, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 392, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405) [M::mem_pestat] mean and std.dev: (404.59, 313.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.58, 69.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (331, 729, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3544) [M::mem_pestat] mean and std.dev: (767.21, 587.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 256, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2499) [M::mem_pestat] mean and std.dev: (585.08, 655.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754770 sequences (100000140 bp) in 3.186 CPU sec, 3.522 real sec... [M::mem_process_seqs] Processed 755892 reads in 113.601 CPU sec, 3.814 real sec sorting block 65, #reads = 1118015 mem = 536878008 peak = 877621248 [M::process] read 754556 sequences (100000096 bp) in 2.977 CPU sec, 3.347 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 303864, 15, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 191, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2578) [M::mem_pestat] mean and std.dev: (471.64, 517.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.12, 69.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 277, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617) [M::mem_pestat] mean and std.dev: (629.67, 777.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 374, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3568) [M::mem_pestat] mean and std.dev: (690.28, 615.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 66, #reads = 1117905 mem = 536878957 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 304020, 22, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 251, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876) [M::mem_pestat] mean and std.dev: (425.05, 419.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.13, 69.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (219, 540, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2625) [M::mem_pestat] mean and std.dev: (716.55, 676.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3427) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 309, 2065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5871) [M::mem_pestat] mean and std.dev: (1014.77, 1329.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754944 sequences (100000266 bp) in 2.963 CPU sec, 3.296 real sec... [M::mem_process_seqs] Processed 754770 reads in 204.647 CPU sec, 6.689 real sec [M::mem_process_seqs] Processed 754556 reads in 139.107 CPU sec, 4.510 real sec [M::process] read 754274 sequences (100000156 bp) in 3.164 CPU sec, 3.414 real sec... sorting block 67, #reads = 1116511 mem = 536875770 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 303717, 18, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 385, 1888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5300) [M::mem_pestat] mean and std.dev: (737.54, 786.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.20, 69.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (451, 1041, 1525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3673) [M::mem_pestat] mean and std.dev: (1005.00, 860.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 214, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2842) [M::mem_pestat] mean and std.dev: (380.13, 349.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 303738, 24, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 349, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3446) [M::mem_pestat] mean and std.dev: (618.32, 680.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (168.44, 69.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 743, 1730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4646) [M::mem_pestat] mean and std.dev: (971.48, 869.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6104) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 160, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (229.85, 140.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751116 sequences (100000040 bp) in 3.055 CPU sec, 3.729 real sec... [M::mem_process_seqs] Processed 754944 reads in 206.004 CPU sec, 6.686 real sec [M::mem_process_seqs] Processed 754274 reads in 139.939 CPU sec, 4.515 real sec sorting block 68, #reads = 1115294 mem = 536879023 peak = 877621248 [M::process] read 750320 sequences (100000185 bp) in 2.967 CPU sec, 3.224 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 302024, 26, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 233, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (254.71, 170.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.19, 71.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 771, 1572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4274) [M::mem_pestat] mean and std.dev: (909.08, 882.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 236, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458) [M::mem_pestat] mean and std.dev: (356.56, 336.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749288 sequences (100000238 bp) in 2.789 CPU sec, 3.198 real sec... sorting block 69, #reads = 1114651 mem = 536878217 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 302351, 19, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 207, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1693) [M::mem_pestat] mean and std.dev: (348.66, 373.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.74, 71.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 452, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4610) [M::mem_pestat] mean and std.dev: (938.61, 1188.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6114) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 218, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (272.93, 193.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 751116 reads in 206.042 CPU sec, 6.655 real sec [M::mem_process_seqs] Processed 750320 reads in 141.950 CPU sec, 4.541 real sec [M::process] read 748822 sequences (100000261 bp) in 2.959 CPU sec, 3.128 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 301242, 19, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 241, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1440) [M::mem_pestat] mean and std.dev: (284.94, 171.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (172.03, 71.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 360, 1138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2986) [M::mem_pestat] mean and std.dev: (688.00, 812.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3939) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 262, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3252) [M::mem_pestat] mean and std.dev: (623.24, 841.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 70, #reads = 1111747 mem = 536879607 peak = 877621248 [M::process] read 749696 sequences (100000151 bp) in 3.045 CPU sec, 3.370 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 301119, 29, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 359, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2187) [M::mem_pestat] mean and std.dev: (490.33, 504.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (172.18, 71.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 582, 1408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3694) [M::mem_pestat] mean and std.dev: (776.72, 633.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 203, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (258.67, 172.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749288 reads in 205.380 CPU sec, 6.669 real sec [M::mem_process_seqs] Processed 748822 reads in 138.501 CPU sec, 4.474 real sec [M::process] read 749124 sequences (100000010 bp) in 3.137 CPU sec, 3.311 real sec... sorting block 71, #reads = 1112692 mem = 536878785 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 301706, 26, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 327, 1117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2985) [M::mem_pestat] mean and std.dev: (508.57, 586.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (171.80, 71.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 363, 2045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5807) [M::mem_pestat] mean and std.dev: (845.70, 1089.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7688) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 200, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974) [M::mem_pestat] mean and std.dev: (431.68, 544.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750126 sequences (100000141 bp) in 3.447 CPU sec, 3.830 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 301871, 18, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 420, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4838) [M::mem_pestat] mean and std.dev: (806.29, 871.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (172.08, 71.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (181, 854, 2486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7096) [M::mem_pestat] mean and std.dev: (1670.78, 2083.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10004) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 241, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3775) [M::mem_pestat] mean and std.dev: (677.12, 719.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749696 reads in 208.789 CPU sec, 6.779 real sec [M::mem_process_seqs] Processed 749124 reads in 143.398 CPU sec, 4.628 real sec sorting block 72, #reads = 1110615 mem = 536879310 peak = 877621248 [M::process] read 749326 sequences (100000227 bp) in 3.023 CPU sec, 3.251 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 302247, 25, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 197, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (227.08, 153.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (171.37, 71.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 683, 2143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6053) [M::mem_pestat] mean and std.dev: (1120.96, 1606.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 235, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (297.89, 225.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749270 sequences (100000065 bp) in 2.995 CPU sec, 3.330 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 301709, 17, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 255, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2965) [M::mem_pestat] mean and std.dev: (493.50, 495.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (171.80, 71.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (337, 777, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5059) [M::mem_pestat] mean and std.dev: (971.12, 974.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 349, 1511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4151) [M::mem_pestat] mean and std.dev: (769.04, 816.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5471) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 73, #reads = 1110817 mem = 536879367 peak = 877621248 [M::mem_process_seqs] Processed 750126 reads in 209.301 CPU sec, 6.859 real sec [M::mem_process_seqs] Processed 749326 reads in 142.346 CPU sec, 4.641 real sec [M::process] read 748902 sequences (100000150 bp) in 3.162 CPU sec, 3.373 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 301569, 21, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 228, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2703) [M::mem_pestat] mean and std.dev: (612.62, 710.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.76, 72.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (180, 490, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2367) [M::mem_pestat] mean and std.dev: (499.21, 468.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3096) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 271, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1939) [M::mem_pestat] mean and std.dev: (358.46, 318.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 74, #reads = 1111999 mem = 536878899 peak = 877621248 [M::process] read 749916 sequences (100000010 bp) in 2.842 CPU sec, 3.307 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 301388, 18, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 377, 1716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4710) [M::mem_pestat] mean and std.dev: (701.22, 843.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.56, 71.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 620, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4600) [M::mem_pestat] mean and std.dev: (981.24, 1340.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 399, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2212) [M::mem_pestat] mean and std.dev: (501.84, 429.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749270 reads in 208.305 CPU sec, 6.747 real sec [M::mem_process_seqs] Processed 748902 reads in 141.445 CPU sec, 4.552 real sec sorting block 75, #reads = 1111113 mem = 536879168 peak = 877621248 [M::process] read 763540 sequences (100000178 bp) in 3.098 CPU sec, 3.338 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 301367, 33, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 328, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023) [M::mem_pestat] mean and std.dev: (409.77, 351.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (171.74, 71.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 475, 1543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4299) [M::mem_pestat] mean and std.dev: (540.71, 554.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5677) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 296, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2525) [M::mem_pestat] mean and std.dev: (421.46, 445.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 762538 sequences (100000271 bp) in 2.985 CPU sec, 3.241 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 306040, 25, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2776) [M::mem_pestat] mean and std.dev: (626.38, 654.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (161.73, 65.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (183, 630, 1983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5583) [M::mem_pestat] mean and std.dev: (853.35, 785.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7383) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 332, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2125) [M::mem_pestat] mean and std.dev: (331.51, 235.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749916 reads in 227.593 CPU sec, 7.324 real sec [M::process] read 761570 sequences (100000169 bp) in 2.414 CPU sec, 2.433 real sec... sorting block 76, #reads = 1109280 mem = 536880431 peak = 877621248 [M::mem_process_seqs] Processed 763540 reads in 158.699 CPU sec, 5.078 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 306028, 20, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 430, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2170) [M::mem_pestat] mean and std.dev: (490.58, 393.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (162.26, 65.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 488, 1723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4839) [M::mem_pestat] mean and std.dev: (978.50, 1171.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6397) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2433) [M::mem_pestat] mean and std.dev: (578.29, 598.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 77, #reads = 1108929 mem = 536878683 peak = 877621248 [M::process] read 760558 sequences (100000078 bp) in 3.182 CPU sec, 3.663 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 306328, 20, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 449, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2129) [M::mem_pestat] mean and std.dev: (508.00, 369.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 152, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (163.08, 66.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (173, 458, 2484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7106) [M::mem_pestat] mean and std.dev: (1442.10, 1859.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 258, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2479) [M::mem_pestat] mean and std.dev: (456.18, 534.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 759098 sequences (100000136 bp) in 2.273 CPU sec, 2.322 real sec... [M::mem_process_seqs] Processed 762538 reads in 224.316 CPU sec, 7.252 real sec [M::mem_process_seqs] Processed 761570 reads in 152.150 CPU sec, 4.879 real sec sorting block 78, #reads = 1114402 mem = 536883592 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 305717, 18, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 271, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2031) [M::mem_pestat] mean and std.dev: (370.35, 385.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 152, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (163.61, 66.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 299, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1608) [M::mem_pestat] mean and std.dev: (464.06, 425.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2166) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 199, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (198.86, 109.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 757898 sequences (100000026 bp) in 3.332 CPU sec, 3.730 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 304588, 23, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 347, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2740) [M::mem_pestat] mean and std.dev: (576.07, 555.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (164.91, 67.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (316, 880, 1390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3538) [M::mem_pestat] mean and std.dev: (897.19, 812.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4612) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 495, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3313) [M::mem_pestat] mean and std.dev: (662.35, 585.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 757454 sequences (100000147 bp) in 2.216 CPU sec, 2.226 real sec... [M::mem_process_seqs] Processed 760558 reads in 220.903 CPU sec, 7.096 real sec [M::mem_process_seqs] Processed 759098 reads in 150.161 CPU sec, 4.807 real sec sorting block 79, #reads = 1112948 mem = 536880862 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 304445, 17, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 371, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2253) [M::mem_pestat] mean and std.dev: (450.04, 366.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 153, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (165.06, 67.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (549, 621, 1765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4197) [M::mem_pestat] mean and std.dev: (810.33, 641.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 205, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1487) [M::mem_pestat] mean and std.dev: (275.29, 245.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 756252 sequences (100000003 bp) in 3.375 CPU sec, 3.807 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 304485, 23, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (257.56, 194.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (165.90, 68.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (123, 314, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (302.75, 211.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1663) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 195, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (353.38, 323.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755148 sequences (100000231 bp) in 2.201 CPU sec, 2.210 real sec... [M::mem_process_seqs] Processed 757898 reads in 217.731 CPU sec, 7.010 real sec sorting block 80, #reads = 1112020 mem = 536884331 peak = 877621248 [M::mem_process_seqs] Processed 757454 reads in 146.606 CPU sec, 4.697 real sec sorting block 81, #reads = 1112334 mem = 536879236 peak = 877621248 [M::process] read 754752 sequences (100000211 bp) in 3.518 CPU sec, 3.805 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 303597, 26, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 206, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4036) [M::mem_pestat] mean and std.dev: (759.53, 1011.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (167.88, 69.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 485, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3121) [M::mem_pestat] mean and std.dev: (716.56, 675.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 302, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2213) [M::mem_pestat] mean and std.dev: (554.00, 530.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755148 reads in 118.231 CPU sec, 3.774 real sec [M::process] read 754024 sequences (100000153 bp) in 2.569 CPU sec, 2.698 real sec... sorting block 82, #reads = 1115462 mem = 536877619 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 304309, 10, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 379, 1287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3579) [M::mem_pestat] mean and std.dev: (755.70, 700.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.22, 68.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 355, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2250) [M::mem_pestat] mean and std.dev: (421.22, 329.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 458, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2378) [M::mem_pestat] mean and std.dev: (586.56, 451.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 303827, 29, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 222, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133) [M::mem_pestat] mean and std.dev: (272.94, 185.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.08, 69.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (120, 464, 1918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5514) [M::mem_pestat] mean and std.dev: (1100.79, 1168.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 346, 2524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7118) [M::mem_pestat] mean and std.dev: (948.80, 1254.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 756252 reads in 327.969 CPU sec, 10.551 real sec [M::mem_process_seqs] Processed 754752 reads in 166.653 CPU sec, 5.330 real sec [M::process] read 754124 sequences (100000295 bp) in 2.545 CPU sec, 2.650 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 303420, 21, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 364, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2784) [M::mem_pestat] mean and std.dev: (651.19, 600.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.34, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 1079, 2850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8326) [M::mem_pestat] mean and std.dev: (1675.05, 2050.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11064) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 203, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1752) [M::mem_pestat] mean and std.dev: (316.34, 297.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2291) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 83, #reads = 1115322 mem = 536878885 peak = 877621248 [M::process] read 752788 sequences (100000098 bp) in 3.435 CPU sec, 3.787 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 303465, 26, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 214, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2978) [M::mem_pestat] mean and std.dev: (566.58, 700.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (168.60, 69.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 469, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1843) [M::mem_pestat] mean and std.dev: (488.12, 388.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 373, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2762) [M::mem_pestat] mean and std.dev: (534.81, 477.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3618) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754024 reads in 204.513 CPU sec, 6.562 real sec [M::mem_process_seqs] Processed 754124 reads in 136.456 CPU sec, 4.321 real sec [M::process] read 751504 sequences (100000218 bp) in 2.489 CPU sec, 2.566 real sec... sorting block 84, #reads = 1114152 mem = 536880914 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 303028, 20, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 182, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3059) [M::mem_pestat] mean and std.dev: (540.96, 588.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.88, 70.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 506, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4142) [M::mem_pestat] mean and std.dev: (865.16, 984.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 467, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1834) [M::mem_pestat] mean and std.dev: (504.35, 379.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2363) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750986 sequences (100000080 bp) in 3.173 CPU sec, 3.396 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 302278, 26, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (181.78, 62.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (170.44, 70.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (417, 1249, 2164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5658) [M::mem_pestat] mean and std.dev: (1533.50, 1308.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 217, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (215.79, 94.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 752788 reads in 196.870 CPU sec, 6.286 real sec [M::process] read 749680 sequences (100000058 bp) in 2.281 CPU sec, 2.328 real sec... sorting block 85, #reads = 1114690 mem = 536879388 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 302159, 18, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 277, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712) [M::mem_pestat] mean and std.dev: (366.57, 345.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (170.70, 70.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 510, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2205) [M::mem_pestat] mean and std.dev: (563.83, 475.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 215, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1823) [M::mem_pestat] mean and std.dev: (283.08, 235.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 750986 reads in 113.042 CPU sec, 3.607 real sec [M::process] read 750078 sequences (100000144 bp) in 2.688 CPU sec, 2.695 real sec... sorting block 86, #reads = 1112867 mem = 536878478 peak = 877621248 [M::mem_process_seqs] Processed 751504 reads in 291.065 CPU sec, 9.276 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 302137, 31, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 419, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4074) [M::mem_pestat] mean and std.dev: (669.67, 770.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (171.45, 70.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 476, 1312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3580) [M::mem_pestat] mean and std.dev: (611.93, 690.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 382, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3628) [M::mem_pestat] mean and std.dev: (681.76, 629.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750072 sequences (100000008 bp) in 3.491 CPU sec, 3.691 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 301858, 17, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 316, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2032) [M::mem_pestat] mean and std.dev: (501.19, 448.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.47, 71.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (173, 324, 3296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9542) [M::mem_pestat] mean and std.dev: (1282.94, 2022.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 710, 1697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4587) [M::mem_pestat] mean and std.dev: (1046.51, 922.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749680 reads in 225.928 CPU sec, 7.223 real sec sorting block 87, #reads = 1111762 mem = 536883055 peak = 877621248 [M::mem_process_seqs] Processed 750078 reads in 137.171 CPU sec, 4.348 real sec [M::process] read 750284 sequences (100000181 bp) in 2.805 CPU sec, 2.955 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 302108, 24, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 328, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4036) [M::mem_pestat] mean and std.dev: (700.73, 818.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.38, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (571, 1247, 3256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8626) [M::mem_pestat] mean and std.dev: (1670.58, 1312.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (370, 639, 1783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4609) [M::mem_pestat] mean and std.dev: (955.34, 861.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 88, #reads = 1110078 mem = 536877854 peak = 877621248 [M::process] read 750576 sequences (100000146 bp) in 2.907 CPU sec, 3.070 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 302159, 31, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 294, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (522.00, 492.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.12, 71.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 764, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3937) [M::mem_pestat] mean and std.dev: (1050.68, 874.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5154) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 257, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (468.44, 437.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2845) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 750072 reads in 203.197 CPU sec, 6.501 real sec [M::process] read 749788 sequences (100000201 bp) in 2.506 CPU sec, 2.606 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 301544, 33, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 370, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4158) [M::mem_pestat] mean and std.dev: (892.37, 1065.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (170.71, 70.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 280, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3028) [M::mem_pestat] mean and std.dev: (580.12, 598.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 268, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1873) [M::mem_pestat] mean and std.dev: (415.10, 351.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 750576 reads in 118.724 CPU sec, 3.783 real sec [M::process] read 753826 sequences (100000196 bp) in 2.886 CPU sec, 2.927 real sec... sorting block 89, #reads = 1109927 mem = 536878801 peak = 877621248 [M::mem_process_seqs] Processed 750284 reads in 314.283 CPU sec, 9.998 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 301465, 34, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 236, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (263.24, 196.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (171.21, 70.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 463, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2725) [M::mem_pestat] mean and std.dev: (526.00, 526.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 269, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2203) [M::mem_pestat] mean and std.dev: (385.00, 361.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 90, #reads = 1112539 mem = 536889919 peak = 877621248 [M::process] read 761992 sequences (100000170 bp) in 3.056 CPU sec, 3.253 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 302439, 24, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 388, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2331) [M::mem_pestat] mean and std.dev: (459.69, 508.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.18, 69.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (141, 420, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3126) [M::mem_pestat] mean and std.dev: (699.96, 726.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4121) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 390, 997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609) [M::mem_pestat] mean and std.dev: (555.49, 466.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749788 reads in 249.805 CPU sec, 7.939 real sec [M::process] read 761406 sequences (100000040 bp) in 2.006 CPU sec, 2.013 real sec... [M::mem_process_seqs] Processed 753826 reads in 163.037 CPU sec, 5.188 real sec sorting block 91, #reads = 1109047 mem = 536879298 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306029, 17, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 207, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2540) [M::mem_pestat] mean and std.dev: (629.32, 681.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (163.10, 66.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (88, 196, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1687) [M::mem_pestat] mean and std.dev: (277.07, 293.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 315, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (303.43, 209.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 760154 sequences (100000260 bp) in 3.463 CPU sec, 3.652 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 306181, 29, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 452, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2958) [M::mem_pestat] mean and std.dev: (519.96, 429.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 152, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (163.23, 66.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (114, 244, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908) [M::mem_pestat] mean and std.dev: (360.28, 320.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 230, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (429.45, 441.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 758802 sequences (100000122 bp) in 2.008 CPU sec, 2.012 real sec... [M::mem_process_seqs] Processed 761992 reads in 203.118 CPU sec, 6.474 real sec [M::mem_process_seqs] Processed 761406 reads in 129.514 CPU sec, 4.112 real sec sorting block 92, #reads = 1106950 mem = 536879464 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 305213, 22, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 280, 1072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2914) [M::mem_pestat] mean and std.dev: (525.94, 579.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (164.13, 66.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (209, 590, 1443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3911) [M::mem_pestat] mean and std.dev: (802.55, 865.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5145) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 360, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3303) [M::mem_pestat] mean and std.dev: (612.72, 524.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 758004 sequences (100000041 bp) in 3.334 CPU sec, 3.467 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 305467, 24, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 257, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418) [M::mem_pestat] mean and std.dev: (285.84, 212.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (164.84, 67.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 413, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4083) [M::mem_pestat] mean and std.dev: (904.21, 925.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 526, 2402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6782) [M::mem_pestat] mean and std.dev: (1230.33, 1516.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 760154 reads in 175.343 CPU sec, 5.619 real sec sorting block 93, #reads = 1118701 mem = 536881116 peak = 877621248 [M::mem_process_seqs] Processed 758802 reads in 117.497 CPU sec, 3.713 real sec [M::process] read 756280 sequences (100000186 bp) in 2.684 CPU sec, 2.817 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 305095, 17, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 561, 1700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4552) [M::mem_pestat] mean and std.dev: (798.30, 739.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (165.36, 67.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (102, 308, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3804) [M::mem_pestat] mean and std.dev: (580.88, 638.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 300, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2056) [M::mem_pestat] mean and std.dev: (398.66, 365.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 94, #reads = 1118637 mem = 536880325 peak = 877621248 [M::process] read 755776 sequences (100000282 bp) in 2.701 CPU sec, 2.867 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 304828, 15, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 175, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246) [M::mem_pestat] mean and std.dev: (319.06, 301.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (167.08, 68.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 294, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3603) [M::mem_pestat] mean and std.dev: (645.27, 557.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 275, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (350.78, 252.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 758004 reads in 183.777 CPU sec, 5.853 real sec [M::process] read 755162 sequences (100000037 bp) in 1.792 CPU sec, 1.806 real sec... [M::mem_process_seqs] Processed 756280 reads in 121.866 CPU sec, 3.859 real sec sorting block 95, #reads = 1119511 mem = 536882769 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 304776, 20, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 318, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2386) [M::mem_pestat] mean and std.dev: (623.41, 592.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.41, 69.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 642, 1457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4199) [M::mem_pestat] mean and std.dev: (730.79, 763.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 276, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473) [M::mem_pestat] mean and std.dev: (387.79, 283.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754810 sequences (100000159 bp) in 3.527 CPU sec, 3.720 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 304311, 16, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 265, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (376.42, 268.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (167.94, 69.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (181, 385, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2158) [M::mem_pestat] mean and std.dev: (555.50, 487.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 438, 1232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3256) [M::mem_pestat] mean and std.dev: (802.10, 835.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755776 reads in 181.327 CPU sec, 5.761 real sec [M::process] read 754222 sequences (100000029 bp) in 2.138 CPU sec, 2.189 real sec... [M::mem_process_seqs] Processed 755162 reads in 123.390 CPU sec, 3.894 real sec sorting block 96, #reads = 1118829 mem = 536882844 peak = 877621248 sorting block 97, #reads = 1118554 mem = 536875983 peak = 877621248 [M::process] read 753866 sequences (100000238 bp) in 3.305 CPU sec, 3.459 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 303553, 21, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 338, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1716) [M::mem_pestat] mean and std.dev: (413.91, 327.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.34, 69.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 531, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4636) [M::mem_pestat] mean and std.dev: (703.45, 798.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 236, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (267.80, 144.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754222 reads in 106.026 CPU sec, 3.332 real sec [M::process] read 753912 sequences (100000070 bp) in 2.253 CPU sec, 2.275 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 303745, 30, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 296, 861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2281) [M::mem_pestat] mean and std.dev: (448.77, 422.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.17, 69.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 674, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379) [M::mem_pestat] mean and std.dev: (869.23, 854.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 287, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2326) [M::mem_pestat] mean and std.dev: (396.18, 383.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 303560, 18, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 363, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (349.93, 257.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (169.08, 70.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 560, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2352) [M::mem_pestat] mean and std.dev: (549.71, 371.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 249, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (378.12, 374.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754810 reads in 296.042 CPU sec, 9.408 real sec [M::mem_process_seqs] Processed 753866 reads in 151.292 CPU sec, 4.815 real sec [M::process] read 754442 sequences (100000062 bp) in 2.837 CPU sec, 2.894 real sec... sorting block 98, #reads = 1115869 mem = 536880311 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 303869, 20, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 405, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3407) [M::mem_pestat] mean and std.dev: (659.11, 713.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (168.92, 69.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 1089, 2672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7626) [M::mem_pestat] mean and std.dev: (1383.45, 1360.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10103) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 319, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1580) [M::mem_pestat] mean and std.dev: (374.58, 348.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 99, #reads = 1116670 mem = 536878960 peak = 877621248 [M::process] read 754388 sequences (100000154 bp) in 3.609 CPU sec, 3.806 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 303716, 19, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 274, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3767) [M::mem_pestat] mean and std.dev: (859.00, 1086.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.17, 69.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 924, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4301) [M::mem_pestat] mean and std.dev: (880.71, 906.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5660) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 269, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665) [M::mem_pestat] mean and std.dev: (300.85, 272.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 753912 reads in 197.108 CPU sec, 6.291 real sec [M::mem_process_seqs] Processed 754442 reads in 131.785 CPU sec, 4.167 real sec [M::process] read 754056 sequences (100000258 bp) in 2.402 CPU sec, 2.484 real sec... sorting block 100, #reads = 1117223 mem = 536884307 peak = 877621248 [M::process] read 754052 sequences (100000292 bp) in 3.082 CPU sec, 3.309 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 302805, 22, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 191, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (283.03, 230.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.14, 69.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 577, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497) [M::mem_pestat] mean and std.dev: (435.14, 293.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1940) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 194, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473) [M::mem_pestat] mean and std.dev: (294.06, 217.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754056 reads in 109.592 CPU sec, 3.458 real sec [M::process] read 753140 sequences (100000253 bp) in 2.208 CPU sec, 2.240 real sec... sorting block 101, #reads = 1115636 mem = 536880431 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 303931, 27, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 254, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2780) [M::mem_pestat] mean and std.dev: (557.24, 688.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.30, 69.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 233, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502) [M::mem_pestat] mean and std.dev: (344.67, 382.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 269, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1819) [M::mem_pestat] mean and std.dev: (315.34, 303.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 302865, 42, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 334, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1743) [M::mem_pestat] mean and std.dev: (401.42, 356.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.05, 68.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 369, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2659) [M::mem_pestat] mean and std.dev: (509.23, 555.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 316, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (369.14, 274.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754388 reads in 298.830 CPU sec, 9.544 real sec [M::mem_process_seqs] Processed 754052 reads in 153.970 CPU sec, 4.909 real sec [M::process] read 752358 sequences (100000023 bp) in 2.662 CPU sec, 2.712 real sec... sorting block 102, #reads = 1116064 mem = 536882349 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 303411, 30, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 603, 1587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4335) [M::mem_pestat] mean and std.dev: (906.21, 787.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.32, 70.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 747, 2301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6549) [M::mem_pestat] mean and std.dev: (1201.46, 1276.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 212, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4103) [M::mem_pestat] mean and std.dev: (667.86, 834.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5424) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751824 sequences (100000015 bp) in 3.184 CPU sec, 3.358 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 302964, 29, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 278, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2442) [M::mem_pestat] mean and std.dev: (318.00, 294.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (170.15, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (490, 992, 1931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4813) [M::mem_pestat] mean and std.dev: (1164.11, 966.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 396, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1591) [M::mem_pestat] mean and std.dev: (427.65, 283.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 753140 reads in 195.814 CPU sec, 6.276 real sec [M::mem_process_seqs] Processed 752358 reads in 133.786 CPU sec, 4.228 real sec [M::process] read 751424 sequences (100000113 bp) in 2.388 CPU sec, 2.452 real sec... sorting block 103, #reads = 1115747 mem = 536880251 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 302752, 20, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 221, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1495) [M::mem_pestat] mean and std.dev: (315.17, 289.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (170.73, 71.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (173, 337, 2648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7598) [M::mem_pestat] mean and std.dev: (1591.95, 2336.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 251, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (286.03, 196.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 752020 sequences (100000175 bp) in 3.037 CPU sec, 3.185 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 302324, 20, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 349, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (306.04, 120.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (170.63, 70.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (150, 661, 1839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5217) [M::mem_pestat] mean and std.dev: (946.40, 897.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6906) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 279, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2997) [M::mem_pestat] mean and std.dev: (643.28, 695.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751768 sequences (100000147 bp) in 1.810 CPU sec, 1.808 real sec... [M::mem_process_seqs] Processed 751824 reads in 188.115 CPU sec, 5.988 real sec sorting block 104, #reads = 1112814 mem = 536878655 peak = 877621248 [M::mem_process_seqs] Processed 751424 reads in 125.009 CPU sec, 3.961 real sec sorting block 105, #reads = 1113549 mem = 536876953 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 302448, 28, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 302, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3151) [M::mem_pestat] mean and std.dev: (618.84, 626.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 157, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (169.14, 69.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 360, 1725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4741) [M::mem_pestat] mean and std.dev: (940.78, 1051.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 701, 1581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4421) [M::mem_pestat] mean and std.dev: (945.67, 921.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 759968 sequences (100000082 bp) in 2.989 CPU sec, 3.109 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 301840, 14, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 182, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2276) [M::mem_pestat] mean and std.dev: (450.62, 493.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (169.96, 70.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 1072, 2590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7686) [M::mem_pestat] mean and std.dev: (1165.08, 1172.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 335, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1495) [M::mem_pestat] mean and std.dev: (346.40, 271.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 764436 sequences (100000091 bp) in 2.220 CPU sec, 2.221 real sec... [M::mem_process_seqs] Processed 752020 reads in 198.155 CPU sec, 6.289 real sec [M::mem_process_seqs] Processed 751768 reads in 138.636 CPU sec, 4.384 real sec sorting block 106, #reads = 1111448 mem = 536880828 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 304529, 23, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 489, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3034) [M::mem_pestat] mean and std.dev: (673.29, 584.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (164.19, 66.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (320, 544, 2216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6008) [M::mem_pestat] mean and std.dev: (990.24, 1054.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7904) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 249, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (240.75, 141.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 763344 sequences (100000279 bp) in 2.615 CPU sec, 2.762 real sec... [M::process] read 762748 sequences (100000161 bp) in 1.788 CPU sec, 1.793 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306394, 29, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 163, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1400) [M::mem_pestat] mean and std.dev: (213.47, 157.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 150, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.46, 65.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 344, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2621) [M::mem_pestat] mean and std.dev: (417.64, 377.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 238, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016) [M::mem_pestat] mean and std.dev: (328.52, 264.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2633) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 759968 reads in 201.059 CPU sec, 6.407 real sec sorting block 107, #reads = 1101846 mem = 536881137 peak = 877621248 [M::mem_process_seqs] Processed 764436 reads in 131.059 CPU sec, 4.179 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 306178, 12, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 313, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3782) [M::mem_pestat] mean and std.dev: (654.40, 777.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 150, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (161.74, 65.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (133, 377, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593) [M::mem_pestat] mean and std.dev: (551.83, 572.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 320, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2151) [M::mem_pestat] mean and std.dev: (477.76, 465.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 762476 sequences (100000052 bp) in 3.137 CPU sec, 3.274 real sec... [M::process] read 761680 sequences (100000053 bp) in 2.229 CPU sec, 2.225 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 306337, 17, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (171.74, 65.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (162.19, 65.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 425, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4180) [M::mem_pestat] mean and std.dev: (769.00, 973.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5534) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 259, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1948) [M::mem_pestat] mean and std.dev: (464.30, 493.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 763344 reads in 203.461 CPU sec, 6.456 real sec sorting block 108, #reads = 1115474 mem = 536878326 peak = 877621248 [M::mem_process_seqs] Processed 762748 reads in 142.699 CPU sec, 4.530 real sec sorting block 109, #reads = 1113073 mem = 536879484 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 306692, 25, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 288, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2306) [M::mem_pestat] mean and std.dev: (488.45, 518.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (162.39, 65.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (83, 573, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3269) [M::mem_pestat] mean and std.dev: (655.40, 592.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4331) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 380, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800) [M::mem_pestat] mean and std.dev: (576.15, 582.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 759500 sequences (100000174 bp) in 3.179 CPU sec, 3.296 real sec... [M::process] read 758254 sequences (100000064 bp) in 2.157 CPU sec, 2.163 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 305359, 26, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 267, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4055) [M::mem_pestat] mean and std.dev: (482.58, 533.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (163.04, 66.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 748, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420) [M::mem_pestat] mean and std.dev: (583.78, 474.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 322, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027) [M::mem_pestat] mean and std.dev: (551.11, 570.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 762476 reads in 203.420 CPU sec, 6.455 real sec [M::mem_process_seqs] Processed 761680 reads in 141.325 CPU sec, 4.498 real sec sorting block 110, #reads = 1115429 mem = 536878079 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 305033, 27, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 377, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2687) [M::mem_pestat] mean and std.dev: (521.26, 537.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (164.51, 66.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 554, 1717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4685) [M::mem_pestat] mean and std.dev: (837.68, 945.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 300, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (249.73, 89.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 757536 sequences (100000054 bp) in 3.419 CPU sec, 3.549 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 304634, 12, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 267, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273) [M::mem_pestat] mean and std.dev: (437.00, 498.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (165.38, 67.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 281, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (407.18, 350.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 286, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (293.76, 217.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 756970 sequences (100000292 bp) in 2.140 CPU sec, 2.137 real sec... [M::mem_process_seqs] Processed 759500 reads in 202.613 CPU sec, 6.433 real sec sorting block 111, #reads = 1112061 mem = 536883166 peak = 877621248 [M::mem_process_seqs] Processed 758254 reads in 136.816 CPU sec, 4.347 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 304360, 31, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 394, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4315) [M::mem_pestat] mean and std.dev: (699.34, 655.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (165.98, 67.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (126, 1068, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3603) [M::mem_pestat] mean and std.dev: (790.76, 695.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 230, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2055) [M::mem_pestat] mean and std.dev: (396.13, 380.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 112, #reads = 1114569 mem = 536879535 peak = 877621248 [M::process] read 756442 sequences (100000128 bp) in 3.129 CPU sec, 3.233 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 304508, 32, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 234, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394) [M::mem_pestat] mean and std.dev: (395.68, 349.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (166.54, 68.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 956, 2948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8088) [M::mem_pestat] mean and std.dev: (1689.66, 1964.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10658) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 282, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1399) [M::mem_pestat] mean and std.dev: (353.07, 236.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755780 sequences (100000029 bp) in 2.180 CPU sec, 2.192 real sec... [M::mem_process_seqs] Processed 757536 reads in 199.182 CPU sec, 6.364 real sec [M::mem_process_seqs] Processed 756970 reads in 135.626 CPU sec, 4.332 real sec sorting block 113, #reads = 1117012 mem = 536883493 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 304356, 23, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 338, 1378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3764) [M::mem_pestat] mean and std.dev: (689.33, 822.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (166.70, 68.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 777, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2565) [M::mem_pestat] mean and std.dev: (717.45, 549.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 271, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5393) [M::mem_pestat] mean and std.dev: (1023.66, 1245.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 756174 sequences (100000197 bp) in 3.300 CPU sec, 3.438 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 303818, 27, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 171, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (185.14, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.27, 68.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (121, 590, 2392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6934) [M::mem_pestat] mean and std.dev: (1019.04, 1353.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 283, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2124) [M::mem_pestat] mean and std.dev: (406.62, 455.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755188 sequences (100000189 bp) in 2.175 CPU sec, 2.176 real sec... [M::mem_process_seqs] Processed 756442 reads in 199.576 CPU sec, 6.359 real sec [M::mem_process_seqs] Processed 755780 reads in 135.769 CPU sec, 4.313 real sec sorting block 114, #reads = 1117184 mem = 536878390 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 304502, 16, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 268, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2638) [M::mem_pestat] mean and std.dev: (486.48, 403.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (166.66, 68.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 597, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4056) [M::mem_pestat] mean and std.dev: (904.75, 947.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 291, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2938) [M::mem_pestat] mean and std.dev: (554.06, 633.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754552 sequences (100000103 bp) in 3.319 CPU sec, 3.447 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 304521, 27, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 354, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3039) [M::mem_pestat] mean and std.dev: (743.36, 854.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (167.96, 69.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 698, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7190) [M::mem_pestat] mean and std.dev: (1116.44, 1372.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 505, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925) [M::mem_pestat] mean and std.dev: (634.34, 532.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754128 sequences (100000118 bp) in 1.996 CPU sec, 2.008 real sec... [M::mem_process_seqs] Processed 756174 reads in 196.954 CPU sec, 6.276 real sec sorting block 115, #reads = 1115414 mem = 536885306 peak = 877621248 [M::mem_process_seqs] Processed 755188 reads in 133.063 CPU sec, 4.225 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 303880, 22, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 473, 1486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4114) [M::mem_pestat] mean and std.dev: (713.64, 756.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.34, 69.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 304, 1672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4916) [M::mem_pestat] mean and std.dev: (911.91, 1063.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 188, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (201.27, 129.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 116, #reads = 1117069 mem = 536887445 peak = 877621248 [M::process] read 753422 sequences (100000190 bp) in 3.336 CPU sec, 3.503 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 303443, 17, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 188, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (300.35, 209.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (169.00, 70.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (461, 718, 1898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4772) [M::mem_pestat] mean and std.dev: (1244.29, 1271.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6332) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 345, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3777) [M::mem_pestat] mean and std.dev: (774.47, 870.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 752822 sequences (100000093 bp) in 2.130 CPU sec, 2.137 real sec... [M::mem_process_seqs] Processed 754552 reads in 193.253 CPU sec, 6.153 real sec [M::mem_process_seqs] Processed 754128 reads in 130.839 CPU sec, 4.146 real sec sorting block 117, #reads = 1116236 mem = 536877712 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 303326, 18, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1624) [M::mem_pestat] mean and std.dev: (421.29, 424.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.34, 70.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 292, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723) [M::mem_pestat] mean and std.dev: (374.88, 408.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 447, 1614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4518) [M::mem_pestat] mean and std.dev: (823.62, 976.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751944 sequences (100000082 bp) in 3.202 CPU sec, 3.382 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 303296, 20, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364) [M::mem_pestat] mean and std.dev: (287.83, 304.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (170.37, 71.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 394, 1496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4130) [M::mem_pestat] mean and std.dev: (554.50, 544.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 271, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1659) [M::mem_pestat] mean and std.dev: (365.22, 262.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751672 sequences (100000200 bp) in 1.755 CPU sec, 1.763 real sec... [M::mem_process_seqs] Processed 753422 reads in 186.441 CPU sec, 5.926 real sec sorting block 118, #reads = 1114484 mem = 536878837 peak = 877621248 [M::mem_process_seqs] Processed 752822 reads in 126.757 CPU sec, 4.029 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 302830, 17, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 356, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3026) [M::mem_pestat] mean and std.dev: (608.39, 534.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.24, 71.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 507, 1298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3260) [M::mem_pestat] mean and std.dev: (491.40, 399.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 306, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985) [M::mem_pestat] mean and std.dev: (544.95, 534.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750556 sequences (100000134 bp) in 3.235 CPU sec, 3.355 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 302913, 33, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 333, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2208) [M::mem_pestat] mean and std.dev: (487.08, 523.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.19, 71.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 796, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2738) [M::mem_pestat] mean and std.dev: (844.06, 834.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 322, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3920) [M::mem_pestat] mean and std.dev: (828.96, 876.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 748944 sequences (100000077 bp) in 2.210 CPU sec, 2.205 real sec... [M::mem_process_seqs] Processed 751944 reads in 191.304 CPU sec, 6.066 real sec sorting block 119, #reads = 1116853 mem = 536878795 peak = 877621248 [M::mem_process_seqs] Processed 751672 reads in 131.210 CPU sec, 4.154 real sec [M::process] read 229196 sequences (30489986 bp) in 1.564 CPU sec, 1.593 real sec... sorting block 120, #reads = 1114414 mem = 536880878 peak = 877621248 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 302350, 20, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 413, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4727) [M::mem_pestat] mean and std.dev: (918.31, 1073.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.89, 71.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 487, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3109) [M::mem_pestat] mean and std.dev: (612.44, 557.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 230, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (321.05, 306.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 301831, 26, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 256, 2047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5761) [M::mem_pestat] mean and std.dev: (811.88, 1246.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.86, 72.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (360, 965, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2898) [M::mem_pestat] mean and std.dev: (882.04, 622.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 223, 1262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3488) [M::mem_pestat] mean and std.dev: (552.76, 577.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4601) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 91954, 14, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 383, 2298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6572) [M::mem_pestat] mean and std.dev: (818.23, 887.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.09, 71.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (137, 482, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1847) [M::mem_pestat] mean and std.dev: (347.42, 237.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2417) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 750556 reads in 206.335 CPU sec, 6.660 real sec [M::mem_process_seqs] Processed 748944 reads in 144.951 CPU sec, 4.688 real sec [M::mem_process_seqs] Processed 229196 reads in 153.242 CPU sec, 4.972 real sec sorting block 121, #reads = 1113444 mem = 536887026 peak = 877621248 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 135831310 overall: 70743760896 mem 22412.420 user 989.719 sys 778.349 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_dm4-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:659_dm4-T1-TDNA-1_A23MHWWLT4_1\tLB:659_dm4-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_dm4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_dm4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 778.377 sec; CPU: 23402.142 sec sorting block 122, #reads = 982987 mem = 475313588 peak = 877621248 uniq header count 1 merging 123 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7227402985 output reads: 137011650 bam_mem_sort: 123 calls 112.839 user 1.783 sys 116.673 real bam_write: 123 calls 80.257 user 7.629 sys 135.119 real execute: 1 calls 44.362 user 3.676 sys 830.059 real merge_files: 1 calls 44.335 user 3.647 sys 50.438 real parse_chunk: 1967 calls 96.194 user 27.587 sys 143.283 real read_chunk: 5342 calls 70.717 user 32.862 sys 690.685 real sort_block: 1 calls 51.492 user 31.355 sys 664.526 real write_chunk: 3373 calls 43.864 user 3.202 sys 49.058 real overall: 3251675136 mem 1982.388 user 112.861 sys 830.077 real ls: cannot access '*.cram': No such file or directory