.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1136_3U6-T1-TDNA-1_A23MHWWLT4_1;L002;0003)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1e/b4c346f88b19f57b5d2db35e6eeab5/.command.log
Size: 128.7 KB
Task: DAQ:SENTIEON_BWAMEM (1136_3U6-T1-TDNA-1_A23MHWWLT4_1;L002;0003)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/92/b72bf64435ce078778748c20d26323/1136_3U6-T1-TDNA-1_A23MHWWLT4_1/0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1e/b4c346f88b19f57b5d2db35e6eeab5/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/92/b72bf64435ce078778748c20d26323/1136_3U6-T1-TDNA-1_A23MHWWLT4_1/0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1e/b4c346f88b19f57b5d2db35e6eeab5/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1640.532 CPU sec, 717.840 real sec
[M::process] read 686966 sequences (100000182 bp) in 0.967 CPU sec, 0.973 real sec...
[M::process] read 685130 sequences (100000037 bp) in 1.184 CPU sec, 1.198 real sec...
[M::process] read 684236 sequences (100000276 bp) in 1.201 CPU sec, 1.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284550, 4, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 320, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (353.69, 179.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 299, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (318.46, 158.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 299, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (243.12, 89.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 687)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683588 sequences (100000206 bp) in 1.168 CPU sec, 1.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284138, 1, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 314, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1604)
[M::mem_pestat] mean and std.dev: (413.25, 277.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2074)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 311, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (328.31, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 346, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (310.94, 111.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283032, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (257, 400, 632)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382)
[M::mem_pestat] mean and std.dev: (374.90, 220.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1757)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 317, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (334.45, 164.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 290, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (292.06, 136.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686966 reads in 219.524 CPU sec, 7.053 real sec
[M::mem_process_seqs] Processed 685130 reads in 145.902 CPU sec, 4.731 real sec
[M::mem_process_seqs] Processed 684236 reads in 157.862 CPU sec, 5.136 real sec
sorting block 0, #reads = 1081713 mem = 536877867 peak = 587251712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282839, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 256, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1392)
[M::mem_pestat] mean and std.dev: (305.78, 169.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1788)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 683040 sequences (100000073 bp) in 2.064 CPU sec, 2.245 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (201, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (337.90, 165.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 397, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (303.21, 123.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683008 sequences (100000132 bp) in 1.409 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282176, 4, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 157, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1332)
[M::mem_pestat] mean and std.dev: (257.50, 230.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1738)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (341.09, 166.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 242, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149)
[M::mem_pestat] mean and std.dev: (316.76, 206.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1460)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683588 reads in 136.455 CPU sec, 4.375 real sec
sorting block 1, #reads = 1080754 mem = 536882364 peak = 825573376
[M::mem_process_seqs] Processed 683040 reads in 85.416 CPU sec, 2.697 real sec
[M::process] read 683134 sequences (100000270 bp) in 1.458 CPU sec, 1.510 real sec...
sorting block 2, #reads = 1080324 mem = 536878689 peak = 902447104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282413, 2, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 364, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (396.73, 225.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1500)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (338.67, 165.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (286, 366, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117)
[M::mem_pestat] mean and std.dev: (435.43, 211.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1394)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682552 sequences (100000127 bp) in 1.851 CPU sec, 2.106 real sec...
[M::process] read 681862 sequences (100000120 bp) in 1.160 CPU sec, 1.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282924, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (326, 473, 1125)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2723)
[M::mem_pestat] mean and std.dev: (531.57, 370.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3522)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (335.99, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 683008 reads in 136.479 CPU sec, 4.342 real sec
[M::mem_process_seqs] Processed 683134 reads in 85.620 CPU sec, 2.723 real sec
sorting block 3, #reads = 1080381 mem = 536879791 peak = 902447104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282334, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 434, 812)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2134)
[M::mem_pestat] mean and std.dev: (434.53, 304.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2795)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (338.44, 164.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 349, 625)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461)
[M::mem_pestat] mean and std.dev: (422.00, 281.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1879)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681716 sequences (100000233 bp) in 1.799 CPU sec, 1.902 real sec...
[M::process] read 681372 sequences (100000096 bp) in 1.180 CPU sec, 1.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 281598, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 283, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 542)
[M::mem_pestat] mean and std.dev: (263.92, 92.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (341.11, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 382, 794)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844)
[M::mem_pestat] mean and std.dev: (474.11, 293.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2369)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682552 reads in 135.763 CPU sec, 4.321 real sec
[M::mem_process_seqs] Processed 681862 reads in 84.176 CPU sec, 2.682 real sec
sorting block 4, #reads = 1079507 mem = 536879688 peak = 902447104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 281921, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 153, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (162.00, 52.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (339.78, 162.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 333, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372)
[M::mem_pestat] mean and std.dev: (358.00, 205.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1760)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680900 sequences (100000245 bp) in 1.848 CPU sec, 1.944 real sec...
[M::process] read 686166 sequences (100000224 bp) in 1.210 CPU sec, 1.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281944, 6, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 171, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (195.81, 82.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 527)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (342.11, 163.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (308, 384, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 689)
[M::mem_pestat] mean and std.dev: (358.19, 132.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681716 reads in 134.075 CPU sec, 4.269 real sec
sorting block 5, #reads = 1079767 mem = 536880183 peak = 902447104
[M::mem_process_seqs] Processed 681372 reads in 83.109 CPU sec, 2.650 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 281934, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 221, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738)
[M::mem_pestat] mean and std.dev: (354.21, 282.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2272)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (347.30, 166.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 325, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1526)
[M::mem_pestat] mean and std.dev: (424.31, 316.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1957)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684958 sequences (100000198 bp) in 1.952 CPU sec, 2.082 real sec...
[M::process] read 683670 sequences (100000259 bp) in 1.250 CPU sec, 1.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283415, 11, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 301, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (331.50, 152.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 305, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (325.27, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (84, 142, 975)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2757)
[M::mem_pestat] mean and std.dev: (415.10, 412.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3648)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 208, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (327.22, 254.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1345)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680900 reads in 133.147 CPU sec, 4.233 real sec
sorting block 6, #reads = 1079807 mem = 536878247 peak = 902447104
[M::mem_process_seqs] Processed 686166 reads in 83.684 CPU sec, 2.663 real sec
sorting block 7, #reads = 1079877 mem = 536884022 peak = 927141888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283284, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 212, 1058)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2768)
[M::mem_pestat] mean and std.dev: (373.56, 309.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3623)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (333.50, 165.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 339, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429)
[M::mem_pestat] mean and std.dev: (421.23, 199.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1823)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683402 sequences (100000077 bp) in 2.010 CPU sec, 2.129 real sec...
[M::process] read 682914 sequences (100000238 bp) in 1.286 CPU sec, 1.295 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282784, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 291, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (376.19, 192.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 321, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (338.00, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 267, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354)
[M::mem_pestat] mean and std.dev: (399.75, 252.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1756)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684958 reads in 132.616 CPU sec, 4.218 real sec
[M::mem_process_seqs] Processed 683670 reads in 82.522 CPU sec, 2.622 real sec
sorting block 8, #reads = 1082738 mem = 536880033 peak = 927141888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282694, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 257, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (328.20, 138.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 323, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (339.69, 166.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 246, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (281.93, 113.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682986 sequences (100000059 bp) in 2.047 CPU sec, 2.177 real sec...
[M::process] read 683170 sequences (100000148 bp) in 1.310 CPU sec, 1.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282633, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 236, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253)
[M::mem_pestat] mean and std.dev: (237.14, 124.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1624)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (343.57, 168.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (303, 942, 1394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3576)
[M::mem_pestat] mean and std.dev: (958.44, 767.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4667)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683402 reads in 133.118 CPU sec, 4.231 real sec
[M::mem_process_seqs] Processed 682914 reads in 83.521 CPU sec, 2.649 real sec
sorting block 9, #reads = 1081857 mem = 536879507 peak = 927141888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282669, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 255, 659)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569)
[M::mem_pestat] mean and std.dev: (403.26, 344.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2024)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (340.24, 166.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (291, 388, 1205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3033)
[M::mem_pestat] mean and std.dev: (458.76, 342.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3947)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683064 sequences (100000113 bp) in 2.142 CPU sec, 2.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282517, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 335, 712)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1806)
[M::mem_pestat] mean and std.dev: (481.72, 449.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2353)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 321, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (338.90, 166.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (296, 369, 818)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862)
[M::mem_pestat] mean and std.dev: (442.40, 222.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2384)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682836 sequences (100000156 bp) in 1.330 CPU sec, 1.329 real sec...
[M::mem_process_seqs] Processed 682986 reads in 135.590 CPU sec, 4.307 real sec
sorting block 10, #reads = 1081840 mem = 536879674 peak = 927141888
[M::mem_process_seqs] Processed 683170 reads in 85.013 CPU sec, 2.697 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282869, 4, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 272, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (360.23, 227.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 321, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (338.73, 165.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 269, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (288.48, 106.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 11, #reads = 1080054 mem = 536879466 peak = 927141888
[M::process] read 682006 sequences (100000047 bp) in 2.229 CPU sec, 2.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282881, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 336, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210)
[M::mem_pestat] mean and std.dev: (279.89, 134.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1557)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (339.58, 165.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 321, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (270.38, 74.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 659)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681638 sequences (100000082 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 683064 reads in 135.070 CPU sec, 4.289 real sec
[M::mem_process_seqs] Processed 682836 reads in 85.002 CPU sec, 2.696 real sec
sorting block 12, #reads = 1079779 mem = 536880948 peak = 927141888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282742, 9, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (296, 398, 807)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1829)
[M::mem_pestat] mean and std.dev: (565.86, 514.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2623)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (343.39, 167.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 439, 738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1810)
[M::mem_pestat] mean and std.dev: (506.42, 338.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2346)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681358 sequences (100000121 bp) in 2.286 CPU sec, 2.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282299, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 296, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (281.40, 125.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (343.40, 166.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 287, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 549)
[M::mem_pestat] mean and std.dev: (300.62, 64.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686580 sequences (100000210 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_process_seqs] Processed 682006 reads in 133.936 CPU sec, 4.253 real sec
[M::mem_process_seqs] Processed 681638 reads in 84.935 CPU sec, 2.688 real sec
sorting block 13, #reads = 1079937 mem = 536881256 peak = 927141888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282264, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 447, 672)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1652)
[M::mem_pestat] mean and std.dev: (440.77, 329.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2142)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (348.45, 168.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 415, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428)
[M::mem_pestat] mean and std.dev: (410.53, 214.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1837)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685086 sequences (100000159 bp) in 2.221 CPU sec, 2.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284406, 9, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 214, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (301.31, 155.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 302, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (320.97, 158.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 262, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (315.55, 152.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684120 sequences (100000024 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 681358 reads in 133.893 CPU sec, 4.251 real sec
[M::mem_process_seqs] Processed 686580 reads in 84.865 CPU sec, 2.687 real sec
sorting block 14, #reads = 1079242 mem = 536880029 peak = 927141888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283264, 6, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 342, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (333.54, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 311, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (328.10, 160.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 226, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (235.24, 77.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683270 sequences (100000238 bp) in 2.224 CPU sec, 2.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283064, 6, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 280, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084)
[M::mem_pestat] mean and std.dev: (320.16, 190.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1387)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 316, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (331.96, 160.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 413, 645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1459)
[M::mem_pestat] mean and std.dev: (467.28, 310.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1866)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682684 sequences (100000192 bp) in 1.370 CPU sec, 1.367 real sec...
[M::mem_process_seqs] Processed 685086 reads in 134.703 CPU sec, 4.277 real sec
sorting block 15, #reads = 1080748 mem = 536881020 peak = 927141888
[M::mem_process_seqs] Processed 684120 reads in 84.414 CPU sec, 2.674 real sec
sorting block 16, #reads = 1081836 mem = 536876904 peak = 1011752960
[M::process] read 682496 sequences (100000117 bp) in 2.320 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282165, 2, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 209, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (275.28, 189.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (340.53, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 315, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (311.79, 137.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682878 sequences (100000265 bp) in 1.370 CPU sec, 1.367 real sec...
[M::mem_process_seqs] Processed 682684 reads in 79.730 CPU sec, 2.521 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282661, 2, 25)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (336.58, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 301, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (299.52, 119.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282339, 6, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (393, 723, 1493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3693)
[M::mem_pestat] mean and std.dev: (815.30, 511.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4793)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (340.06, 164.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 274, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1182)
[M::mem_pestat] mean and std.dev: (343.54, 186.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1500)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682772 sequences (100000087 bp) in 1.544 CPU sec, 1.547 real sec...
[M::mem_process_seqs] Processed 683270 reads in 212.611 CPU sec, 6.755 real sec
sorting block 17, #reads = 1079744 mem = 536877144 peak = 1011752960
[M::mem_process_seqs] Processed 682496 reads in 100.339 CPU sec, 3.198 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282180, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 198, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (229.00, 120.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (338.41, 164.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 462, 1185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3059)
[M::mem_pestat] mean and std.dev: (430.46, 271.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3996)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1079311 mem = 536880682 peak = 1011752960
[M::process] read 682356 sequences (100000050 bp) in 2.134 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282193, 0, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 193, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238)
[M::mem_pestat] mean and std.dev: (260.84, 183.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1599)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (338.03, 163.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 215, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (240.27, 124.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682016 sequences (100000257 bp) in 1.340 CPU sec, 1.335 real sec...
[M::mem_process_seqs] Processed 682878 reads in 140.678 CPU sec, 4.477 real sec
[M::mem_process_seqs] Processed 682772 reads in 88.161 CPU sec, 2.796 real sec
sorting block 19, #reads = 1079283 mem = 536879545 peak = 1011752960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282459, 12, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 391, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428)
[M::mem_pestat] mean and std.dev: (378.45, 277.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1853)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (340.88, 165.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1072, 1750, 1750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3106)
[M::mem_pestat] mean and std.dev: (1323.75, 607.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3784)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 384, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195)
[M::mem_pestat] mean and std.dev: (330.97, 128.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1507)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681500 sequences (100000134 bp) in 2.193 CPU sec, 2.337 real sec...
[M::process] read 682098 sequences (100000184 bp) in 1.370 CPU sec, 1.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281999, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 307, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (321.55, 191.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (342.59, 165.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (315, 384, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (137, 582)
[M::mem_pestat] mean and std.dev: (360.18, 95.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682356 reads in 139.629 CPU sec, 4.434 real sec
[M::mem_process_seqs] Processed 682016 reads in 86.920 CPU sec, 2.755 real sec
sorting block 20, #reads = 1081071 mem = 536880382 peak = 1011752960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282524, 1, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 355, 562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288)
[M::mem_pestat] mean and std.dev: (372.52, 205.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1651)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (346.97, 167.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 317, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (317.00, 134.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686330 sequences (100000008 bp) in 2.183 CPU sec, 2.299 real sec...
[M::process] read 684988 sequences (100000281 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282866, 9, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 177, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477)
[M::mem_pestat] mean and std.dev: (178.14, 53.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 583)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (343.79, 166.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 263, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (308.75, 165.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1387)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681500 reads in 135.072 CPU sec, 4.290 real sec
[M::mem_process_seqs] Processed 682098 reads in 83.618 CPU sec, 2.654 real sec
sorting block 21, #reads = 1079382 mem = 536878512 peak = 1011752960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283657, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 200, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (224.41, 79.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 582)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 305, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (326.32, 164.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (358, 373, 1174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2806)
[M::mem_pestat] mean and std.dev: (701.42, 508.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3622)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684318 sequences (100000253 bp) in 2.184 CPU sec, 2.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282836, 4, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 281, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (336.00, 132.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 313, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (332.03, 165.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 333, 572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1392)
[M::mem_pestat] mean and std.dev: (445.27, 360.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1889)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683952 sequences (100000252 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_process_seqs] Processed 686330 reads in 135.918 CPU sec, 4.319 real sec
sorting block 22, #reads = 1080053 mem = 536879324 peak = 1011752960
[M::mem_process_seqs] Processed 684988 reads in 85.305 CPU sec, 2.707 real sec
sorting block 23, #reads = 1082880 mem = 536880936 peak = 1041584128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283132, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 343, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170)
[M::mem_pestat] mean and std.dev: (315.53, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 318, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (335.71, 165.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (332, 360, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 635)
[M::mem_pestat] mean and std.dev: (336.70, 112.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683220 sequences (100000281 bp) in 2.239 CPU sec, 2.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282836, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 327, 1238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3244)
[M::mem_pestat] mean and std.dev: (647.93, 585.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4247)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (336.17, 165.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 282, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (283.67, 100.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682944 sequences (100000095 bp) in 1.370 CPU sec, 1.372 real sec...
[M::mem_process_seqs] Processed 684318 reads in 137.415 CPU sec, 4.373 real sec
[M::mem_process_seqs] Processed 683952 reads in 85.938 CPU sec, 2.725 real sec
sorting block 24, #reads = 1081886 mem = 536878604 peak = 1041584128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282185, 2, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 237, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970)
[M::mem_pestat] mean and std.dev: (405.58, 368.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (340.06, 166.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 316, 1163)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3033)
[M::mem_pestat] mean and std.dev: (593.11, 543.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3968)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683056 sequences (100000106 bp) in 2.221 CPU sec, 2.357 real sec...
[M::process] read 683018 sequences (100000002 bp) in 1.360 CPU sec, 1.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 281945, 8, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 269, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (297.95, 202.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 323, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (339.38, 165.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 374, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (326.78, 140.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683220 reads in 140.278 CPU sec, 4.464 real sec
[M::mem_process_seqs] Processed 682944 reads in 87.930 CPU sec, 2.789 real sec
sorting block 25, #reads = 1081611 mem = 536877930 peak = 1041584128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282708, 5, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (341, 508, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (410.77, 156.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1333)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (337.94, 164.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 332, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281)
[M::mem_pestat] mean and std.dev: (354.77, 170.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1638)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682690 sequences (100000025 bp) in 2.257 CPU sec, 2.381 real sec...
[M::process] read 681898 sequences (100000017 bp) in 1.350 CPU sec, 1.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282034, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 248, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (247.25, 67.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 684)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (337.55, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 337, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (313.59, 114.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683056 reads in 140.797 CPU sec, 4.480 real sec
[M::mem_process_seqs] Processed 683018 reads in 87.836 CPU sec, 2.787 real sec
sorting block 26, #reads = 1079943 mem = 536881549 peak = 1041584128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282398, 5, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 213, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (259.50, 116.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (338.54, 163.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 280, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 522)
[M::mem_pestat] mean and std.dev: (278.81, 75.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681630 sequences (100000004 bp) in 2.354 CPU sec, 2.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282030, 9, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 225, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (248.44, 76.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (338.48, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 388, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412)
[M::mem_pestat] mean and std.dev: (411.46, 217.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1814)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684488 sequences (100000110 bp) in 1.380 CPU sec, 1.377 real sec...
[M::mem_process_seqs] Processed 682690 reads in 138.935 CPU sec, 4.416 real sec
sorting block 27, #reads = 1080411 mem = 536876316 peak = 1041584128
[M::mem_process_seqs] Processed 681898 reads in 86.467 CPU sec, 2.743 real sec
sorting block 28, #reads = 1079718 mem = 536881306 peak = 1041584128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282260, 1, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 178, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (212.95, 81.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (342.38, 163.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 213, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437)
[M::mem_pestat] mean and std.dev: (322.60, 242.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1872)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689002 sequences (100000271 bp) in 2.345 CPU sec, 2.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282534, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 258, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125)
[M::mem_pestat] mean and std.dev: (377.36, 277.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1487)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (334.90, 167.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (348.14, 128.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687152 sequences (100000071 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 681630 reads in 137.632 CPU sec, 4.376 real sec
[M::mem_process_seqs] Processed 684488 reads in 88.005 CPU sec, 2.785 real sec
sorting block 29, #reads = 1078738 mem = 536878053 peak = 1041584128
[M::process] read 685602 sequences (100000118 bp) in 2.297 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284090, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 563, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279)
[M::mem_pestat] mean and std.dev: (346.05, 191.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1626)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 301, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (324.19, 164.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684584 sequences (100000268 bp) in 1.380 CPU sec, 1.377 real sec...
[M::mem_process_seqs] Processed 687152 reads in 76.413 CPU sec, 2.411 real sec
sorting block 30, #reads = 1080132 mem = 536877400 peak = 1041584128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285088, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 245, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108)
[M::mem_pestat] mean and std.dev: (254.25, 144.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1427)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 291, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (317.96, 164.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 402, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (384.79, 148.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283606, 9, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 260, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (229.19, 84.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 312, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (332.41, 167.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684036 sequences (100000109 bp) in 1.775 CPU sec, 1.784 real sec...
[M::mem_process_seqs] Processed 689002 reads in 219.501 CPU sec, 6.966 real sec
[M::mem_process_seqs] Processed 685602 reads in 104.904 CPU sec, 3.348 real sec
sorting block 31, #reads = 1082683 mem = 536879060 peak = 1041584128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283500, 8, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077)
[M::mem_pestat] mean and std.dev: (333.41, 144.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1366)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (337.13, 167.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 264, 971)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2581)
[M::mem_pestat] mean and std.dev: (478.50, 410.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3386)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683572 sequences (100000123 bp) in 2.276 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283810, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (261, 348, 1130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2868)
[M::mem_pestat] mean and std.dev: (590.68, 523.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3737)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 322, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (340.36, 169.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 327, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (320.90, 138.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1364)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683234 sequences (100000294 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_process_seqs] Processed 684584 reads in 149.973 CPU sec, 4.784 real sec
[M::mem_process_seqs] Processed 684036 reads in 94.339 CPU sec, 2.995 real sec
sorting block 32, #reads = 1078956 mem = 536878345 peak = 1041584128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282983, 7, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 254, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (290.33, 114.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 322, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (339.64, 167.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 358, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (310.33, 117.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683256 sequences (100000178 bp) in 2.437 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282770, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 308, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313)
[M::mem_pestat] mean and std.dev: (355.50, 237.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1700)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682748 sequences (100000197 bp) in 1.440 CPU sec, 1.438 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (339.94, 166.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 289, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1598)
[M::mem_pestat] mean and std.dev: (460.80, 388.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2073)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683572 reads in 151.712 CPU sec, 4.827 real sec
[M::mem_process_seqs] Processed 683234 reads in 94.661 CPU sec, 3.002 real sec
sorting block 33, #reads = 1078587 mem = 536877750 peak = 1041584128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282749, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 215, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447)
[M::mem_pestat] mean and std.dev: (194.00, 69.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 542)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (339.14, 165.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 197, 688)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1800)
[M::mem_pestat] mean and std.dev: (315.80, 265.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2356)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683028 sequences (100000006 bp) in 2.356 CPU sec, 2.504 real sec...
[M::process] read 684562 sequences (100000036 bp) in 1.450 CPU sec, 1.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282325, 7, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (355, 448, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (451.31, 136.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (337.45, 162.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 512, 811)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1915)
[M::mem_pestat] mean and std.dev: (502.67, 339.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2467)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683256 reads in 150.976 CPU sec, 4.803 real sec
sorting block 34, #reads = 1077328 mem = 536879001 peak = 1041584128
[M::mem_process_seqs] Processed 682748 reads in 93.851 CPU sec, 2.978 real sec
sorting block 35, #reads = 1076639 mem = 536880571 peak = 1067839488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283161, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 260, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (262.41, 150.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (337.98, 163.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 412, 3435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9767)
[M::mem_pestat] mean and std.dev: (1992.18, 2743.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12964)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690930 sequences (100000203 bp) in 2.356 CPU sec, 2.491 real sec...
[M::process] read 688230 sequences (100000283 bp) in 1.490 CPU sec, 1.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283810, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 277, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243)
[M::mem_pestat] mean and std.dev: (304.43, 195.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (334.60, 167.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 683028 reads in 151.594 CPU sec, 4.819 real sec
[M::mem_process_seqs] Processed 684562 reads in 95.209 CPU sec, 3.024 real sec
sorting block 36, #reads = 1076656 mem = 536883014 peak = 1067839488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 286338, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 277, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (266.81, 98.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 279, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (306.57, 157.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (529, 538, 635)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (317, 847)
[M::mem_pestat] mean and std.dev: (529.56, 63.31)
[M::mem_pestat] low and high boundaries for proper pairs: (211, 953)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687780 sequences (100000184 bp) in 2.500 CPU sec, 2.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285497, 6, 34)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 293, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (316.24, 159.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 301, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (298.38, 145.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RR
[M::process] read 686042 sequences (100000076 bp) in 1.470 CPU sec, 1.462 real sec...
[M::mem_process_seqs] Processed 690930 reads in 153.680 CPU sec, 4.889 real sec
[M::mem_process_seqs] Processed 688230 reads in 95.613 CPU sec, 3.038 real sec
sorting block 37, #reads = 1078464 mem = 536878594 peak = 1067839488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 285129, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 298, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (340.77, 195.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 296, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (319.30, 160.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 310, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (333.36, 177.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685198 sequences (100000182 bp) in 2.461 CPU sec, 2.610 real sec...
[M::process] read 684196 sequences (100000085 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284322, 10, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 249, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (263.87, 107.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 307, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (326.77, 162.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 629, 1103)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2889)
[M::mem_pestat] mean and std.dev: (616.50, 436.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3782)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 373, 758)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832)
[M::mem_pestat] mean and std.dev: (321.86, 176.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2369)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687780 reads in 154.168 CPU sec, 4.905 real sec
sorting block 38, #reads = 1078971 mem = 536878820 peak = 1067839488
[M::mem_process_seqs] Processed 686042 reads in 95.056 CPU sec, 3.020 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284005, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 377, 875)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247)
[M::mem_pestat] mean and std.dev: (496.23, 354.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2933)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 39, #reads = 1077540 mem = 536884008 peak = 1067839488
[M::mem_pestat] (25, 50, 75) percentile: (195, 315, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (333.29, 165.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 221, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (236.76, 142.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683830 sequences (100000192 bp) in 2.377 CPU sec, 2.516 real sec...
[M::process] read 683272 sequences (100000261 bp) in 1.460 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283910, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 167, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (176.50, 44.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 533)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (337.25, 166.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 336, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1374)
[M::mem_pestat] mean and std.dev: (374.94, 210.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1754)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685198 reads in 154.952 CPU sec, 4.932 real sec
[M::mem_process_seqs] Processed 684196 reads in 96.578 CPU sec, 3.066 real sec
sorting block 40, #reads = 1077237 mem = 536877137 peak = 1067839488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283558, 4, 26)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 320, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (339.02, 167.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 189, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (216.18, 103.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RR
[M::process] read 683068 sequences (100000117 bp) in 2.575 CPU sec, 2.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283039, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 319, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1306)
[M::mem_pestat] mean and std.dev: (297.26, 193.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1687)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (342.35, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 250, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (255.90, 64.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683830 reads in 153.867 CPU sec, 4.908 real sec
[M::process] read 682534 sequences (100000201 bp) in 1.450 CPU sec, 1.450 real sec...
[M::mem_process_seqs] Processed 683272 reads in 96.651 CPU sec, 3.059 real sec
sorting block 41, #reads = 1077911 mem = 536879580 peak = 1067839488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283266, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 165, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1255)
[M::mem_pestat] mean and std.dev: (273.55, 221.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1620)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 326, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (345.29, 171.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 352, 689)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1693)
[M::mem_pestat] mean and std.dev: (432.82, 285.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2195)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687904 sequences (100000037 bp) in 2.501 CPU sec, 2.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282663, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (252, 404, 831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1989)
[M::mem_pestat] mean and std.dev: (513.24, 361.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 331, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (351.24, 176.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 241, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (275.05, 176.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687384 sequences (100000078 bp) in 1.433 CPU sec, 1.441 real sec...
[M::mem_process_seqs] Processed 683068 reads in 151.016 CPU sec, 4.804 real sec
[M::mem_process_seqs] Processed 682534 reads in 95.926 CPU sec, 3.043 real sec
sorting block 42, #reads = 1077702 mem = 536880122 peak = 1067839488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283838, 1, 30)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 295, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (321.23, 165.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 393, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331)
[M::mem_pestat] mean and std.dev: (382.31, 174.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1694)
[M::mem_pestat] skip orientation RR
[M::process] read 686728 sequences (100000143 bp) in 2.410 CPU sec, 2.540 real sec...
[M::process] read 685554 sequences (100000294 bp) in 1.440 CPU sec, 1.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284315, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 235, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (253.20, 87.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 298, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (319.60, 160.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (287, 333, 1048)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2570)
[M::mem_pestat] mean and std.dev: (606.58, 417.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3331)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687904 reads in 155.649 CPU sec, 4.952 real sec
sorting block 43, #reads = 1076382 mem = 536876843 peak = 1067839488
[M::mem_process_seqs] Processed 687384 reads in 93.400 CPU sec, 2.976 real sec
sorting block 44, #reads = 1078859 mem = 536880922 peak = 1081102336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283937, 6, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 303, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (331.21, 187.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 301, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (322.69, 161.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 332, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (287.74, 99.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685402 sequences (100000209 bp) in 2.181 CPU sec, 2.313 real sec...
[M::process] read 684712 sequences (100000019 bp) in 1.460 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283839, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 338, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103)
[M::mem_pestat] mean and std.dev: (295.73, 147.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1405)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (329.64, 164.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 308, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (161, 441)
[M::mem_pestat] mean and std.dev: (285.00, 55.19)
[M::mem_pestat] low and high boundaries for proper pairs: (64, 506)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686728 reads in 153.003 CPU sec, 4.882 real sec
[M::mem_process_seqs] Processed 685554 reads in 94.861 CPU sec, 3.013 real sec
sorting block 45, #reads = 1080656 mem = 536878154 peak = 1081102336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283698, 1, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 240, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 408)
[M::mem_pestat] mean and std.dev: (234.05, 69.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 312, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (330.86, 164.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 549, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668)
[M::mem_pestat] mean and std.dev: (534.05, 361.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2135)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683744 sequences (100000206 bp) in 3.051 CPU sec, 3.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283137, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 335, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (323.55, 184.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 317, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (335.10, 165.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 279, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081)
[M::mem_pestat] mean and std.dev: (255.25, 93.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1361)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685402 reads in 159.305 CPU sec, 5.068 real sec
[M::mem_process_seqs] Processed 684712 reads in 99.559 CPU sec, 3.141 real sec
[M::process] read 683538 sequences (100000132 bp) in 1.992 CPU sec, 2.025 real sec...
sorting block 46, #reads = 1079192 mem = 536879507 peak = 1081102336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283130, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 557, 846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112)
[M::mem_pestat] mean and std.dev: (550.93, 321.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2745)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (338.49, 165.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 359, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (282.29, 91.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683670 sequences (100000027 bp) in 3.019 CPU sec, 3.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282700, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 381, 834)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080)
[M::mem_pestat] mean and std.dev: (542.64, 379.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2703)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (338.39, 165.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 307, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (271.79, 113.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683744 reads in 160.609 CPU sec, 5.114 real sec
[M::mem_process_seqs] Processed 683538 reads in 99.345 CPU sec, 3.136 real sec
[M::process] read 683266 sequences (100000043 bp) in 2.071 CPU sec, 2.131 real sec...
sorting block 47, #reads = 1078119 mem = 536878808 peak = 1081102336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283216, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 198, 594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418)
[M::mem_pestat] mean and std.dev: (364.79, 354.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1830)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (337.91, 166.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 599, 1485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3905)
[M::mem_pestat] mean and std.dev: (952.58, 1010.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5115)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 48, #reads = 1076094 mem = 536879386 peak = 1081102336
[M::process] read 683530 sequences (100000260 bp) in 3.108 CPU sec, 3.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282761, 8, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 198, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (268.90, 167.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (340.00, 166.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 309, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (317.78, 138.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683670 reads in 160.958 CPU sec, 5.136 real sec
[M::mem_process_seqs] Processed 683266 reads in 100.639 CPU sec, 3.178 real sec
[M::process] read 691054 sequences (100000086 bp) in 2.424 CPU sec, 2.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283178, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 263, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1620)
[M::mem_pestat] mean and std.dev: (375.20, 342.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2102)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 322, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (341.08, 169.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 373, 1694)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4556)
[M::mem_pestat] mean and std.dev: (632.13, 690.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5987)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 49, #reads = 1074375 mem = 536878523 peak = 1081102336
[M::process] read 689680 sequences (100000226 bp) in 2.577 CPU sec, 2.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 286157, 11, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 451, 1014)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2596)
[M::mem_pestat] mean and std.dev: (701.50, 659.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3387)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 276, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (304.13, 156.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (404, 914, 1897)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4883)
[M::mem_pestat] mean and std.dev: (1162.82, 1067.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6376)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 286, 1050)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2674)
[M::mem_pestat] mean and std.dev: (645.86, 580.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3486)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683530 reads in 166.347 CPU sec, 5.304 real sec
[M::mem_process_seqs] Processed 691054 reads in 106.739 CPU sec, 3.373 real sec
[M::process] read 689844 sequences (100000202 bp) in 1.933 CPU sec, 1.986 real sec...
sorting block 50, #reads = 1072579 mem = 536883671 peak = 1081102336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285750, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 291, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (232.47, 93.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 285, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (309.52, 156.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (222.33, 105.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1345)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687728 sequences (100000046 bp) in 2.884 CPU sec, 3.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 286271, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 330, 635)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541)
[M::mem_pestat] mean and std.dev: (496.96, 450.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2300)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 282, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (307.63, 155.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 468, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763)
[M::mem_pestat] mean and std.dev: (441.71, 316.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2278)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689680 reads in 159.742 CPU sec, 5.091 real sec
[M::mem_process_seqs] Processed 689844 reads in 101.681 CPU sec, 3.210 real sec
[M::process] read 686398 sequences (100000133 bp) in 1.905 CPU sec, 1.965 real sec...
sorting block 51, #reads = 1077829 mem = 536878844 peak = 1081102336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285211, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 260, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1579)
[M::mem_pestat] mean and std.dev: (382.00, 363.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2044)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 293, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (318.08, 160.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 220, 817)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2077)
[M::mem_pestat] mean and std.dev: (383.67, 377.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2707)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684688 sequences (100000164 bp) in 2.688 CPU sec, 2.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284386, 2, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 239, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434)
[M::mem_pestat] mean and std.dev: (369.52, 258.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1847)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 305, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (326.51, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 345, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112)
[M::mem_pestat] mean and std.dev: (383.37, 198.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1396)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684202 sequences (100000292 bp) in 1.590 CPU sec, 1.590 real sec...
[M::mem_process_seqs] Processed 687728 reads in 161.131 CPU sec, 5.120 real sec
sorting block 52, #reads = 1076839 mem = 536879302 peak = 1081102336
[M::mem_process_seqs] Processed 686398 reads in 100.845 CPU sec, 3.190 real sec
sorting block 53, #reads = 1076524 mem = 536882436 peak = 1081102336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283306, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (266, 310, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (345.79, 166.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (332.66, 163.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 356, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (339.07, 148.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1328)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683346 sequences (100000024 bp) in 2.605 CPU sec, 2.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283249, 7, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 206, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (217.80, 80.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682718 sequences (100000141 bp) in 1.540 CPU sec, 1.541 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (198, 316, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (335.28, 165.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 402, 1072)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2826)
[M::mem_pestat] mean and std.dev: (501.45, 392.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3703)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684688 reads in 159.262 CPU sec, 5.059 real sec
[M::mem_process_seqs] Processed 684202 reads in 98.438 CPU sec, 3.114 real sec
sorting block 54, #reads = 1075500 mem = 536879103 peak = 1081102336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282350, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 361, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1481)
[M::mem_pestat] mean and std.dev: (451.83, 312.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1901)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (338.94, 165.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 284, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (271.46, 125.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682244 sequences (100000225 bp) in 2.798 CPU sec, 2.943 real sec...
[M::process] read 213156 sequences (31219711 bp) in 0.760 CPU sec, 0.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282798, 8, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 415, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318)
[M::mem_pestat] mean and std.dev: (444.21, 227.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1671)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (340.89, 166.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (313, 381, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (357.78, 131.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683346 reads in 156.474 CPU sec, 4.991 real sec
[M::mem_process_seqs] Processed 682718 reads in 96.039 CPU sec, 3.059 real sec
sorting block 55, #reads = 1076149 mem = 536878749 peak = 1081102336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281942, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 226, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (231.86, 133.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (345.71, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 643, 716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1762)
[M::mem_pestat] mean and std.dev: (514.62, 409.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2285)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 88309, 1, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 323, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (341.82, 168.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 447, 954)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2462)
[M::mem_pestat] mean and std.dev: (761.47, 800.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3963)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682244 reads in 104.673 CPU sec, 3.402 real sec
[M::mem_process_seqs] Processed 213156 reads in 39.309 CPU sec, 1.277 real sec
sorting block 56, #reads = 1076856 mem = 536878959 peak = 1081102336
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 61792406
overall: 69898362880 mem 8452.806 user 336.273 sys 953.693 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 953.729 sec; CPU: 8789.082 sec
sorting block 57, #reads = 613673 mem = 306392474 peak = 1081102336
uniq header count 1
merging 58 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3598745918
output reads: 62122624
bam_mem_sort: 58 calls 23.563 user 0.312 sys 24.034 real
bam_write: 58 calls 27.347 user 0.939 sys 28.484 real
execute: 1 calls 13.586 user 0.840 sys 973.950 real
merge_files: 1 calls 13.575 user 0.811 sys 19.677 real
parse_chunk: 922 calls 31.236 user 8.538 sys 40.493 real
read_chunk: 2487 calls 22.146 user 21.927 sys 235.687 real
sort_block: 1 calls 18.575 user 21.284 sys 228.385 real
write_chunk: 1563 calls 13.217 user 0.647 sys 18.960 real
overall: 2673840128 mem 752.968 user 40.993 sys 973.972 real
ls: cannot access '*.cram': No such file or directory