cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NTC_0002_0015_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1869.701 CPU sec, 72.832 real sec
[M::process] read 4994 sequences (751978 bp) in 0.005 CPU sec, 0.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 87, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 270, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (254.13, 112.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 4994 reads in 13.395 CPU sec, 0.446 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
signature: 000c06f2
features: bfebfbff 7ffefbff
extended: f3bfb7ef fb417ffe
amd bits: 2c100800 00000121
brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 4994
overall: 68433817600 mem 1632.899 user 260.779 sys 83.882 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.NTC_0002_0015_A23MHWWLT4_1.L007\tPU:L007\tSM:NTC_0002_0015_A23MHWWLT4_1\tLB:NTC_0002_0015_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NTC_0002_0015_A23MHWWLT4_1_R1.fastq.gz NTC_0002_0015_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 83.932 sec; CPU: 1893.681 sec
sorting block 0, #reads = 5131 mem = 2837221 peak = 77160448
uniq header count 1
merging 1 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
signature: 000c06f2
features: bfebfbff 7ffefbff
extended: f3bfb7ef fb417ffe
amd bits: 2c100800 00000121
brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 374978
output reads: 5131
bam_mem_sort: 1 calls 0.000 user 0.000 sys 0.002 real
bam_write: 1 calls 0.000 user 0.008 sys 0.006 real
execute: 1 calls 0.079 user 0.148 sys 84.098 real
merge_files: 1 calls 0.036 user 0.121 sys 0.178 real
parse_chunk: 1 calls 0.000 user 0.000 sys 0.005 real
read_chunk: 4 calls 0.004 user 0.004 sys 10.582 real
sort_block: 1 calls 0.009 user 0.008 sys 10.591 real
write_chunk: 1 calls 0.000 user 0.001 sys 0.002 real
overall: 265584640 mem 0.268 user 0.153 sys 84.123 real
ls: cannot access '*.cram': No such file or directory