.command.log - Workdir - DAQ:SENTIEON_BWAMEM (659_cAd-T1-TDNA-1_A23MHWWLT4_1;L001;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/70/75e712fdb88739a4c910957826fc89/.command.log
Size: 203.0 KB
Task: DAQ:SENTIEON_BWAMEM (659_cAd-T1-TDNA-1_A23MHWWLT4_1;L001;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/70/75e712fdb88739a4c910957826fc89/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c1/da64fa346dbf9be4ef889e8f23daa7/659_cAd-T1-TDNA-1_A23MHWWLT4_1/659_cAd-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/70/75e712fdb88739a4c910957826fc89/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c1/da64fa346dbf9be4ef889e8f23daa7/659_cAd-T1-TDNA-1_A23MHWWLT4_1/659_cAd-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cAd-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2257.845 CPU sec, 94.636 real sec
[M::process] read 683316 sequences (100000047 bp) in 1.296 CPU sec, 1.298 real sec...
[M::process] read 682884 sequences (100000120 bp) in 1.628 CPU sec, 1.643 real sec...
[M::process] read 681986 sequences (100000111 bp) in 1.595 CPU sec, 1.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287440, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (314.67, 144.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681674 sequences (100000225 bp) in 1.548 CPU sec, 1.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287186, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (317.33, 144.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683316 reads in 190.650 CPU sec, 6.523 real sec
[M::mem_process_seqs] Processed 682884 reads in 112.835 CPU sec, 3.808 real sec
sorting block 0, #reads = 1086247 mem = 536881065 peak = 590204928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286495, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (321.45, 146.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681324 sequences (100000048 bp) in 2.017 CPU sec, 2.273 real sec...
[M::process] read 681346 sequences (100000073 bp) in 1.725 CPU sec, 1.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287118, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 311, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (324.25, 147.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681986 reads in 171.394 CPU sec, 5.829 real sec
[M::mem_process_seqs] Processed 681674 reads in 112.302 CPU sec, 4.051 real sec
sorting block 1, #reads = 1084725 mem = 536878371 peak = 711315456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286526, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 313, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (325.37, 147.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681030 sequences (100000123 bp) in 1.759 CPU sec, 2.140 real sec...
[M::process] read 680528 sequences (100000138 bp) in 1.641 CPU sec, 1.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286885, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 314, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (326.44, 148.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681324 reads in 169.226 CPU sec, 5.540 real sec
[M::mem_process_seqs] Processed 681346 reads in 108.806 CPU sec, 3.553 real sec
sorting block 2, #reads = 1085932 mem = 536878834 peak = 831848448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287333, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 315, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (326.91, 147.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680482 sequences (100000164 bp) in 2.076 CPU sec, 2.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286347, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (329.07, 147.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680368 sequences (100000168 bp) in 1.640 CPU sec, 1.651 real sec...
[M::mem_process_seqs] Processed 681030 reads in 168.430 CPU sec, 5.522 real sec
[M::mem_process_seqs] Processed 680528 reads in 109.466 CPU sec, 3.602 real sec
sorting block 3, #reads = 1086214 mem = 536880864 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286801, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 320, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (331.76, 149.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1085402 mem = 536881477 peak = 884416512
[M::process] read 681352 sequences (100000037 bp) in 2.292 CPU sec, 2.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286793, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 321, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (332.64, 150.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680482 reads in 169.269 CPU sec, 5.611 real sec
[M::process] read 681376 sequences (100000211 bp) in 1.726 CPU sec, 1.749 real sec...
[M::mem_process_seqs] Processed 680368 reads in 110.634 CPU sec, 3.604 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286930, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 314, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (327.13, 149.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1085807 mem = 536881066 peak = 884416512
[M::process] read 681292 sequences (100000287 bp) in 2.367 CPU sec, 2.653 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286795, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 313, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (326.30, 148.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681088 sequences (100000049 bp) in 1.649 CPU sec, 1.663 real sec...
[M::mem_process_seqs] Processed 681352 reads in 171.927 CPU sec, 5.724 real sec
[M::mem_process_seqs] Processed 681376 reads in 112.092 CPU sec, 3.798 real sec
sorting block 6, #reads = 1086668 mem = 536883642 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286691, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 313, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (326.73, 148.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681176 sequences (100000020 bp) in 2.139 CPU sec, 2.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286472, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 313, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (326.49, 148.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681292 reads in 171.805 CPU sec, 5.672 real sec
[M::mem_process_seqs] Processed 681088 reads in 110.925 CPU sec, 3.617 real sec
[M::process] read 680878 sequences (100000243 bp) in 1.817 CPU sec, 1.998 real sec...
sorting block 7, #reads = 1087063 mem = 536884144 peak = 884416512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286133, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (326.11, 147.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680612 sequences (100000206 bp) in 2.282 CPU sec, 2.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286503, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 313, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (326.08, 147.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1009, 1305, 8558)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23656)
[M::mem_pestat] mean and std.dev: (2984.27, 3443.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 31205)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 681176 reads in 171.568 CPU sec, 5.629 real sec
[M::process] read 680796 sequences (100000106 bp) in 1.727 CPU sec, 1.838 real sec...
[M::mem_process_seqs] Processed 680878 reads in 110.119 CPU sec, 3.602 real sec
sorting block 8, #reads = 1085398 mem = 536877404 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286553, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (326.01, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1085844 mem = 536882273 peak = 905916416
[M::process] read 680484 sequences (100000256 bp) in 2.272 CPU sec, 2.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286238, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (323.63, 145.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680612 reads in 171.276 CPU sec, 5.605 real sec
[M::process] read 681508 sequences (100000252 bp) in 1.948 CPU sec, 2.028 real sec...
[M::mem_process_seqs] Processed 680796 reads in 109.967 CPU sec, 3.514 real sec
sorting block 10, #reads = 1085195 mem = 536877686 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286391, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 309, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (322.58, 144.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681792 sequences (100000160 bp) in 2.400 CPU sec, 2.686 real sec...
[M::process] read 680984 sequences (100000016 bp) in 1.746 CPU sec, 1.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286422, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (315.51, 139.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680484 reads in 179.799 CPU sec, 5.775 real sec
[M::mem_process_seqs] Processed 681508 reads in 118.343 CPU sec, 3.785 real sec
sorting block 11, #reads = 1085314 mem = 536878103 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287078, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 303, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (314.99, 140.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680978 sequences (100000124 bp) in 2.535 CPU sec, 2.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286265, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 305, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (319.09, 143.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680668 sequences (100000202 bp) in 2.056 CPU sec, 2.183 real sec...
[M::mem_process_seqs] Processed 681792 reads in 187.653 CPU sec, 6.123 real sec
sorting block 12, #reads = 1081997 mem = 536881274 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286503, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (319.84, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680978 reads in 95.360 CPU sec, 3.072 real sec
[M::process] read 680578 sequences (100000034 bp) in 2.604 CPU sec, 2.677 real sec...
[M::mem_process_seqs] Processed 680984 reads in 262.419 CPU sec, 8.519 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286375, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 307, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (320.25, 143.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (311, 2605, 4149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11825)
[M::mem_pestat] mean and std.dev: (2894.50, 2216.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15663)
[M::mem_pestat] skip orientation RR
sorting block 13, #reads = 1085172 mem = 536879411 peak = 905916416
[M::process] read 680654 sequences (100000112 bp) in 2.419 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286540, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 306, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (319.80, 142.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680668 reads in 185.194 CPU sec, 5.991 real sec
[M::mem_process_seqs] Processed 680578 reads in 109.081 CPU sec, 3.521 real sec
[M::process] read 680090 sequences (100000139 bp) in 2.158 CPU sec, 2.324 real sec...
sorting block 14, #reads = 1085507 mem = 536882055 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286475, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 308, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (321.16, 143.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680242 sequences (100000264 bp) in 2.535 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285866, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 309, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (322.23, 143.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680654 reads in 168.714 CPU sec, 5.468 real sec
[M::mem_process_seqs] Processed 680090 reads in 110.550 CPU sec, 3.535 real sec
sorting block 15, #reads = 1085040 mem = 536878807 peak = 905916416
[M::process] read 680034 sequences (100000169 bp) in 2.500 CPU sec, 2.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286687, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 310, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (322.48, 143.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679818 sequences (100000138 bp) in 2.686 CPU sec, 2.964 real sec...
sorting block 16, #reads = 1084135 mem = 536883851 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286513, 13, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 310, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (322.00, 142.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (451, 451, 1816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4546)
[M::mem_pestat] mean and std.dev: (1021.69, 1119.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5911)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 163, 3012)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8710)
[M::mem_pestat] mean and std.dev: (1118.75, 1338.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11559)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680242 reads in 173.457 CPU sec, 5.606 real sec
[M::mem_process_seqs] Processed 680034 reads in 113.251 CPU sec, 3.653 real sec
[M::process] read 680956 sequences (100000265 bp) in 2.262 CPU sec, 2.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285864, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 310, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (324.00, 144.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1083751 mem = 536879546 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286697, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 307, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (320.10, 143.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682412 sequences (100000196 bp) in 3.081 CPU sec, 3.363 real sec...
[M::mem_process_seqs] Processed 679818 reads in 177.915 CPU sec, 5.796 real sec
[M::mem_process_seqs] Processed 680956 reads in 115.905 CPU sec, 3.745 real sec
[M::process] read 682078 sequences (100000095 bp) in 2.677 CPU sec, 2.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286765, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 728, 811)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913)
[M::mem_pestat] mean and std.dev: (455.50, 277.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2464)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (318.16, 144.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (791, 1101, 1101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (171, 1721)
[M::mem_pestat] mean and std.dev: (929.00, 313.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2183)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1084538 mem = 536880957 peak = 905916416
[M::process] read 681682 sequences (100000283 bp) in 2.224 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286944, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (320.68, 145.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682412 reads in 169.492 CPU sec, 5.498 real sec
[M::mem_process_seqs] Processed 682078 reads in 112.135 CPU sec, 3.637 real sec
[M::process] read 681218 sequences (100000000 bp) in 2.818 CPU sec, 3.192 real sec...
sorting block 19, #reads = 1085090 mem = 536879314 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286380, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (323.27, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681116 sequences (100000038 bp) in 2.445 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286594, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 313, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (326.82, 149.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681682 reads in 174.854 CPU sec, 5.770 real sec
[M::mem_process_seqs] Processed 681218 reads in 112.964 CPU sec, 3.783 real sec
sorting block 20, #reads = 1086258 mem = 536878933 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286842, 7, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 315, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (328.74, 149.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (203.69, 89.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RR
[M::process] read 681064 sequences (100000118 bp) in 3.032 CPU sec, 3.312 real sec...
[M::mem_process_seqs] Processed 681116 reads in 95.634 CPU sec, 3.297 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286812, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 317, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (329.60, 150.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680528 sequences (100000048 bp) in 2.290 CPU sec, 2.848 real sec...
sorting block 21, #reads = 1085163 mem = 536876944 peak = 905916416
[M::mem_process_seqs] Processed 681064 reads in 92.645 CPU sec, 3.292 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286469, 13, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 318, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (330.53, 149.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 73, 873)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2489)
[M::mem_pestat] mean and std.dev: (370.15, 398.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3297)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 680302 sequences (100000154 bp) in 2.440 CPU sec, 2.865 real sec...
[M::mem_process_seqs] Processed 680528 reads in 94.589 CPU sec, 3.240 real sec
sorting block 22, #reads = 1085834 mem = 536878700 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286773, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 321, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (333.48, 150.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680318 sequences (100000118 bp) in 2.632 CPU sec, 2.861 real sec...
[M::mem_process_seqs] Processed 680302 reads in 92.841 CPU sec, 3.203 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286845, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 323, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (335.98, 152.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679910 sequences (100000218 bp) in 2.845 CPU sec, 3.146 real sec...
[M::mem_process_seqs] Processed 680318 reads in 94.657 CPU sec, 3.214 real sec
sorting block 23, #reads = 1085749 mem = 536879237 peak = 905916416
[M::process] read 681284 sequences (100000079 bp) in 2.222 CPU sec, 2.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286243, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 337, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (248.20, 91.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 323, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (335.19, 151.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 679910 reads in 95.457 CPU sec, 3.353 real sec
sorting block 24, #reads = 1086180 mem = 536879339 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287096, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 313, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (327.25, 149.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680910 sequences (100000171 bp) in 2.727 CPU sec, 3.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287077, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (465, 465, 2279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5907)
[M::mem_pestat] mean and std.dev: (1145.50, 896.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7721)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 315, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (328.43, 149.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680784 sequences (100000047 bp) in 2.825 CPU sec, 3.154 real sec...
[M::mem_process_seqs] Processed 680910 reads in 97.721 CPU sec, 3.259 real sec
sorting block 25, #reads = 1085210 mem = 536879646 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286431, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 314, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (327.50, 148.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680782 sequences (100000032 bp) in 2.646 CPU sec, 2.989 real sec...
[M::mem_process_seqs] Processed 680784 reads in 95.658 CPU sec, 3.218 real sec
[M::mem_process_seqs] Processed 681284 reads in 289.151 CPU sec, 9.923 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286259, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 314, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (327.33, 149.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680792 sequences (100000168 bp) in 2.851 CPU sec, 3.344 real sec...
sorting block 26, #reads = 1086480 mem = 536879194 peak = 905916416
[M::mem_process_seqs] Processed 680782 reads in 99.396 CPU sec, 3.419 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286782, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (526, 875, 981)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891)
[M::mem_pestat] mean and std.dev: (701.27, 283.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2346)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (326.38, 148.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680840 sequences (100000288 bp) in 2.539 CPU sec, 2.989 real sec...
[M::mem_process_seqs] Processed 680792 reads in 96.310 CPU sec, 3.317 real sec
sorting block 27, #reads = 1086260 mem = 536880223 peak = 905916416
[M::process] read 681048 sequences (100000232 bp) in 2.353 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285806, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (324.26, 146.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 207, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (254.22, 111.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680840 reads in 98.626 CPU sec, 3.349 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286560, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (323.08, 146.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681348 sequences (100000135 bp) in 2.840 CPU sec, 3.118 real sec...
[M::mem_process_seqs] Processed 681048 reads in 97.124 CPU sec, 3.268 real sec
sorting block 28, #reads = 1086225 mem = 536879159 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287099, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (320.65, 145.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1240, 3397, 3745)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8755)
[M::mem_pestat] mean and std.dev: (2438.00, 1342.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11260)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 681362 sequences (100000038 bp) in 2.944 CPU sec, 3.235 real sec...
[M::mem_process_seqs] Processed 681348 reads in 101.188 CPU sec, 3.415 real sec
sorting block 29, #reads = 1085316 mem = 536885030 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287074, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (320.88, 146.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682546 sequences (100000174 bp) in 2.975 CPU sec, 3.299 real sec...
[M::mem_process_seqs] Processed 681362 reads in 98.674 CPU sec, 3.325 real sec
[M::process] read 681274 sequences (100000043 bp) in 2.753 CPU sec, 3.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287197, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (315.64, 143.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 30, #reads = 1085480 mem = 536877618 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287194, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (319.75, 144.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680890 sequences (100000034 bp) in 2.891 CPU sec, 3.168 real sec...
[M::mem_process_seqs] Processed 682546 reads in 192.734 CPU sec, 6.352 real sec
[M::mem_process_seqs] Processed 681274 reads in 119.276 CPU sec, 3.978 real sec
sorting block 31, #reads = 1083200 mem = 536877714 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286433, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (321.36, 145.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680906 sequences (100000213 bp) in 2.529 CPU sec, 2.806 real sec...
[M::mem_process_seqs] Processed 680890 reads in 97.855 CPU sec, 3.288 real sec
[M::process] read 680856 sequences (100000048 bp) in 2.724 CPU sec, 2.990 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286194, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (321.17, 145.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680906 reads in 99.473 CPU sec, 3.412 real sec
sorting block 32, #reads = 1085122 mem = 536878959 peak = 905916416
[M::process] read 680498 sequences (100000072 bp) in 2.439 CPU sec, 2.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286272, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 307, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (320.96, 144.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680426 sequences (100000181 bp) in 2.342 CPU sec, 2.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286678, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (322.07, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1085311 mem = 536879831 peak = 905916416
[M::mem_process_seqs] Processed 680856 reads in 174.916 CPU sec, 5.752 real sec
[M::mem_process_seqs] Processed 680498 reads in 113.842 CPU sec, 3.746 real sec
[M::process] read 680352 sequences (100000242 bp) in 2.852 CPU sec, 3.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285996, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 309, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (321.23, 142.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1085311 mem = 536877340 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286336, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 309, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (322.28, 143.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679916 sequences (100000189 bp) in 2.666 CPU sec, 3.006 real sec...
[M::mem_process_seqs] Processed 680426 reads in 175.609 CPU sec, 5.761 real sec
[M::mem_process_seqs] Processed 680352 reads in 114.269 CPU sec, 3.752 real sec
[M::process] read 679970 sequences (100000290 bp) in 2.568 CPU sec, 2.749 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286379, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 311, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (324.11, 144.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1084863 mem = 536880548 peak = 905916416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286261, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 312, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (324.84, 144.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680112 sequences (100000254 bp) in 2.700 CPU sec, 2.958 real sec...
[M::mem_process_seqs] Processed 679916 reads in 175.328 CPU sec, 5.684 real sec
[M::mem_process_seqs] Processed 679970 reads in 112.934 CPU sec, 3.682 real sec
sorting block 36, #reads = 1084544 mem = 536877190 peak = 905916416
[M::process] read 682762 sequences (100000162 bp) in 2.826 CPU sec, 3.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286143, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (325.92, 146.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (711, 711, 3128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7962)
[M::mem_pestat] mean and std.dev: (1686.70, 1660.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10379)
[M::mem_pestat] skip orientation RR
[M::process] read 682136 sequences (100000270 bp) in 2.487 CPU sec, 2.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287048, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (318.08, 145.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680112 reads in 179.464 CPU sec, 5.835 real sec
[M::mem_process_seqs] Processed 682762 reads in 117.680 CPU sec, 3.815 real sec
sorting block 37, #reads = 1084709 mem = 536878944 peak = 905916416
[M::process] read 681486 sequences (100000265 bp) in 2.367 CPU sec, 2.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287594, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (321.30, 146.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286398, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (323.37, 147.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681090 sequences (100000076 bp) in 2.817 CPU sec, 3.108 real sec...
sorting block 38, #reads = 1084532 mem = 536884934 peak = 924946432
[M::mem_process_seqs] Processed 682136 reads in 178.946 CPU sec, 5.811 real sec
[M::mem_process_seqs] Processed 681486 reads in 116.631 CPU sec, 3.746 real sec
[M::process] read 680688 sequences (100000021 bp) in 2.856 CPU sec, 3.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286802, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 313, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (325.70, 147.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1085227 mem = 536878476 peak = 924946432
[M::process] read 680390 sequences (100000040 bp) in 2.429 CPU sec, 2.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286562, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 317, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (329.52, 149.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681090 reads in 175.139 CPU sec, 5.693 real sec
[M::mem_process_seqs] Processed 680688 reads in 114.254 CPU sec, 3.715 real sec
[M::process] read 680128 sequences (100000274 bp) in 2.356 CPU sec, 2.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286272, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 317, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (329.82, 148.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1084194 mem = 536880751 peak = 924946432
[M::process] read 679856 sequences (100000136 bp) in 2.718 CPU sec, 3.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286689, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 321, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (332.88, 150.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680390 reads in 169.607 CPU sec, 5.486 real sec
[M::mem_process_seqs] Processed 680128 reads in 111.152 CPU sec, 3.559 real sec
sorting block 41, #reads = 1085594 mem = 536877525 peak = 924946432
[M::process] read 679778 sequences (100000025 bp) in 2.774 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286585, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 322, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (333.64, 149.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (618, 618, 1139)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181)
[M::mem_pestat] mean and std.dev: (582.62, 286.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2702)
[M::mem_pestat] skip orientation RR
[M::process] read 679632 sequences (100000214 bp) in 2.598 CPU sec, 2.805 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286667, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 324, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (337.06, 153.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679856 reads in 169.385 CPU sec, 5.493 real sec
[M::mem_process_seqs] Processed 679778 reads in 111.442 CPU sec, 3.599 real sec
sorting block 42, #reads = 1085592 mem = 536879339 peak = 924946432
[M::process] read 680778 sequences (100000245 bp) in 2.343 CPU sec, 2.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286913, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 325, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (338.17, 153.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286920, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 318, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (331.32, 151.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1085093 mem = 536877756 peak = 925630464
[M::process] read 681434 sequences (100000068 bp) in 2.451 CPU sec, 2.951 real sec...
[M::mem_process_seqs] Processed 679632 reads in 170.109 CPU sec, 5.567 real sec
[M::mem_process_seqs] Processed 680778 reads in 110.347 CPU sec, 3.562 real sec
[M::process] read 680820 sequences (100000292 bp) in 2.723 CPU sec, 3.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287090, 3, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 309, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (324.41, 148.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (375, 658, 1432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3546)
[M::mem_pestat] mean and std.dev: (746.33, 627.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4603)
[M::mem_pestat] skip orientation RR
sorting block 44, #reads = 1086469 mem = 536881060 peak = 925630464
[M::process] read 680322 sequences (100000175 bp) in 2.374 CPU sec, 2.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287057, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 314, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (327.22, 148.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 513, 805)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2031)
[M::mem_pestat] mean and std.dev: (527.00, 431.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2644)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681434 reads in 174.709 CPU sec, 5.704 real sec
[M::mem_process_seqs] Processed 680820 reads in 111.167 CPU sec, 3.641 real sec
[M::process] read 680756 sequences (100000215 bp) in 2.466 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286665, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 314, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (327.77, 148.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (313, 399, 1094)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2656)
[M::mem_pestat] mean and std.dev: (584.50, 417.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3437)
[M::mem_pestat] skip orientation RR
sorting block 45, #reads = 1086376 mem = 536878565 peak = 925630464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286762, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (325.91, 148.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680684 sequences (100000255 bp) in 2.702 CPU sec, 2.987 real sec...
[M::mem_process_seqs] Processed 680322 reads in 173.199 CPU sec, 5.663 real sec
[M::mem_process_seqs] Processed 680756 reads in 110.872 CPU sec, 3.619 real sec
sorting block 46, #reads = 1085399 mem = 536882773 peak = 925630464
[M::process] read 680802 sequences (100000288 bp) in 2.785 CPU sec, 3.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286236, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (324.81, 147.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680684 reads in 97.475 CPU sec, 3.296 real sec
[M::process] read 680768 sequences (100000251 bp) in 2.606 CPU sec, 2.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286477, 13, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (323.80, 147.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (417, 2901, 2901)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7869)
[M::mem_pestat] mean and std.dev: (1706.08, 1341.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10353)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 680802 reads in 94.076 CPU sec, 3.278 real sec
sorting block 47, #reads = 1085401 mem = 536879444 peak = 925630464
[M::process] read 680760 sequences (100000090 bp) in 2.548 CPU sec, 2.937 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286530, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (323.27, 146.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (451, 452, 985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2053)
[M::mem_pestat] mean and std.dev: (635.55, 484.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2587)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286410, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 334, 735)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1805)
[M::mem_pestat] mean and std.dev: (347.75, 236.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2340)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (322.44, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681828 sequences (100000122 bp) in 2.767 CPU sec, 3.098 real sec...
sorting block 48, #reads = 1085275 mem = 536879461 peak = 925630464
[M::mem_process_seqs] Processed 680768 reads in 174.978 CPU sec, 5.851 real sec
[M::mem_process_seqs] Processed 680760 reads in 113.027 CPU sec, 3.865 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286305, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 681276 sequences (100000073 bp) in 2.846 CPU sec, 3.188 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (318.61, 145.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681828 reads in 102.332 CPU sec, 3.555 real sec
sorting block 49, #reads = 1085954 mem = 536881720 peak = 925630464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286774, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (320.87, 145.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (429, 477, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (333, 573)
[M::mem_pestat] mean and std.dev: (459.00, 23.24)
[M::mem_pestat] low and high boundaries for proper pairs: (285, 621)
[M::mem_pestat] skip orientation RR
[M::process] read 680906 sequences (100000019 bp) in 2.786 CPU sec, 3.088 real sec...
[M::mem_process_seqs] Processed 681276 reads in 96.937 CPU sec, 3.349 real sec
[M::process] read 680850 sequences (100000210 bp) in 2.614 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286925, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (323.14, 146.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1084707 mem = 536878389 peak = 925630464
[M::mem_process_seqs] Processed 680906 reads in 100.969 CPU sec, 3.428 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285958, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (319, 346, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (265, 400)
[M::mem_pestat] mean and std.dev: (335.29, 17.85)
[M::mem_pestat] low and high boundaries for proper pairs: (238, 427)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 680758 sequences (100000059 bp) in 2.739 CPU sec, 3.279 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (322.85, 147.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 680850 reads in 97.545 CPU sec, 3.389 real sec
sorting block 51, #reads = 1085783 mem = 536878517 peak = 925630464
[M::process] read 680606 sequences (100000127 bp) in 2.911 CPU sec, 3.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286649, 6, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (323.86, 147.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 245, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (287.77, 102.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286002, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (323.49, 146.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680460 sequences (100000131 bp) in 2.594 CPU sec, 2.816 real sec...
[M::mem_process_seqs] Processed 680758 reads in 179.210 CPU sec, 5.854 real sec
[M::mem_process_seqs] Processed 680606 reads in 112.053 CPU sec, 3.610 real sec
sorting block 52, #reads = 1084827 mem = 536878128 peak = 925630464
[M::process] read 680260 sequences (100000261 bp) in 2.471 CPU sec, 2.781 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286526, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 310, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (325.21, 147.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1086018 mem = 536877681 peak = 953651200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286441, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (324.58, 146.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680248 sequences (100000218 bp) in 2.798 CPU sec, 3.053 real sec...
[M::mem_process_seqs] Processed 680460 reads in 179.671 CPU sec, 5.854 real sec
[M::mem_process_seqs] Processed 680260 reads in 114.598 CPU sec, 3.724 real sec
[M::process] read 680634 sequences (100000300 bp) in 2.763 CPU sec, 3.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285891, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (325.45, 147.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1085186 mem = 536882332 peak = 953651200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286689, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (323.75, 146.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679880 sequences (100000026 bp) in 2.614 CPU sec, 2.895 real sec...
[M::mem_process_seqs] Processed 680248 reads in 171.850 CPU sec, 5.652 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286341, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 313, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (327.36, 148.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681366 sequences (100000053 bp) in 2.327 CPU sec, 2.786 real sec...
sorting block 55, #reads = 1084018 mem = 536878841 peak = 953651200
[M::mem_process_seqs] Processed 679880 reads in 100.834 CPU sec, 3.497 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286764, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (320.42, 145.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681852 sequences (100000181 bp) in 2.763 CPU sec, 3.147 real sec...
[M::mem_process_seqs] Processed 680634 reads in 302.247 CPU sec, 10.133 real sec
[M::mem_process_seqs] Processed 681366 reads in 100.952 CPU sec, 3.327 real sec
sorting block 56, #reads = 1083902 mem = 536878240 peak = 953651200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 287081, 0, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 163, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (283.29, 163.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (321.33, 147.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681786 sequences (100000209 bp) in 2.707 CPU sec, 2.982 real sec...
[M::mem_process_seqs] Processed 681852 reads in 91.090 CPU sec, 3.092 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286914, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (750, 750, 750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (750, 750)
[M::mem_pestat] mean and std.dev: (750.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (750, 750)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (323.84, 148.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681166 sequences (100000113 bp) in 2.476 CPU sec, 2.762 real sec...
[M::mem_process_seqs] Processed 681786 reads in 95.085 CPU sec, 3.228 real sec
sorting block 57, #reads = 1084014 mem = 536886024 peak = 953651200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286767, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 314, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (328.15, 150.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680858 sequences (100000107 bp) in 2.905 CPU sec, 3.105 real sec...
[M::mem_process_seqs] Processed 681166 reads in 92.761 CPU sec, 3.107 real sec
sorting block 58, #reads = 1087012 mem = 536878014 peak = 991514624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286705, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (314, 362, 820)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832)
[M::mem_pestat] mean and std.dev: (449.17, 283.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2338)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 316, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (329.10, 150.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680398 sequences (100000211 bp) in 2.469 CPU sec, 2.858 real sec...
[M::mem_process_seqs] Processed 680858 reads in 93.592 CPU sec, 3.185 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286030, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 317, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (331.71, 151.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680400 sequences (100000294 bp) in 2.861 CPU sec, 3.076 real sec...
[M::mem_process_seqs] Processed 680398 reads in 94.616 CPU sec, 3.190 real sec
sorting block 59, #reads = 1085427 mem = 536878177 peak = 991514624
[M::process] read 679828 sequences (100000111 bp) in 2.521 CPU sec, 2.749 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286571, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 320, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (332.43, 151.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (456, 676, 1555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3753)
[M::mem_pestat] mean and std.dev: (836.55, 562.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4852)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286001, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 321, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (333.70, 150.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1086181 mem = 536883115 peak = 991514624
[M::mem_process_seqs] Processed 680400 reads in 176.500 CPU sec, 5.794 real sec
[M::process] read 679380 sequences (100000296 bp) in 3.175 CPU sec, 3.535 real sec...
[M::mem_process_seqs] Processed 679828 reads in 117.454 CPU sec, 3.794 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286366, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 323, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (335.68, 151.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 1415, 1415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3745)
[M::mem_pestat] mean and std.dev: (818.10, 597.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4910)
[M::mem_pestat] skip orientation RR
[M::process] read 679492 sequences (100000027 bp) in 2.549 CPU sec, 2.871 real sec...
[M::mem_process_seqs] Processed 679380 reads in 95.223 CPU sec, 3.204 real sec
sorting block 61, #reads = 1085384 mem = 536878991 peak = 991514624
[M::process] read 679368 sequences (100000016 bp) in 2.949 CPU sec, 3.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286063, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 324, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (336.90, 152.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286479, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 327, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (339.53, 153.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (951, 951, 1005)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (843, 1113)
[M::mem_pestat] mean and std.dev: (958.71, 18.90)
[M::mem_pestat] low and high boundaries for proper pairs: (789, 1167)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 679492 reads in 171.115 CPU sec, 5.600 real sec
[M::process] read 681110 sequences (100000202 bp) in 2.630 CPU sec, 3.147 real sec...
[M::mem_process_seqs] Processed 679368 reads in 110.155 CPU sec, 3.579 real sec
sorting block 62, #reads = 1084871 mem = 536878421 peak = 991514624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286902, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 314, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (329.44, 151.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680962 sequences (100000198 bp) in 2.859 CPU sec, 3.049 real sec...
[M::mem_process_seqs] Processed 681110 reads in 97.144 CPU sec, 3.267 real sec
sorting block 63, #reads = 1085713 mem = 536881315 peak = 991514624
[M::process] read 680730 sequences (100000153 bp) in 2.544 CPU sec, 2.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286698, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 311, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (326.76, 150.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286280, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 315, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (329.27, 150.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680516 sequences (100000090 bp) in 2.576 CPU sec, 2.831 real sec...
[M::mem_process_seqs] Processed 680962 reads in 174.754 CPU sec, 5.682 real sec
[M::mem_process_seqs] Processed 680730 reads in 113.810 CPU sec, 3.682 real sec
sorting block 64, #reads = 1085476 mem = 536878924 peak = 991514624
[M::process] read 680894 sequences (100000228 bp) in 2.630 CPU sec, 2.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286707, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 314, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (328.05, 149.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286288, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (325.30, 148.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 65, #reads = 1086195 mem = 536876723 peak = 991514624
[M::process] read 680796 sequences (100000295 bp) in 2.730 CPU sec, 3.045 real sec...
[M::mem_process_seqs] Processed 680516 reads in 175.343 CPU sec, 5.704 real sec
[M::mem_process_seqs] Processed 680894 reads in 114.645 CPU sec, 3.700 real sec
[M::process] read 680842 sequences (100000183 bp) in 2.460 CPU sec, 2.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286557, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (324.03, 147.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 66, #reads = 1085378 mem = 536881174 peak = 991514624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286629, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (323.19, 146.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680934 sequences (100000057 bp) in 2.607 CPU sec, 2.947 real sec...
[M::mem_process_seqs] Processed 680796 reads in 174.243 CPU sec, 5.684 real sec
[M::mem_process_seqs] Processed 680842 reads in 111.873 CPU sec, 3.649 real sec
[M::process] read 680756 sequences (100000106 bp) in 2.749 CPU sec, 2.952 real sec...
sorting block 67, #reads = 1086234 mem = 536878496 peak = 991514624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286815, 2, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (295, 295, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (359.50, 159.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1467)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (322.69, 146.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286444, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (323.18, 146.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680648 sequences (100000065 bp) in 2.655 CPU sec, 3.019 real sec...
[M::mem_process_seqs] Processed 680934 reads in 177.765 CPU sec, 5.758 real sec
[M::mem_process_seqs] Processed 680756 reads in 115.981 CPU sec, 3.716 real sec
sorting block 68, #reads = 1085307 mem = 536879704 peak = 991514624
[M::process] read 680642 sequences (100000273 bp) in 2.547 CPU sec, 2.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286516, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (323.59, 146.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680742 sequences (100000063 bp) in 2.587 CPU sec, 2.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286462, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (324.14, 147.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680648 reads in 176.465 CPU sec, 5.699 real sec
sorting block 69, #reads = 1085987 mem = 536879116 peak = 991514624
[M::mem_process_seqs] Processed 680642 reads in 114.862 CPU sec, 3.660 real sec
[M::process] read 680998 sequences (100000276 bp) in 2.603 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286538, 3, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 174, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1259)
[M::mem_pestat] mean and std.dev: (317.00, 252.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1629)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 309, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (323.92, 147.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 70, #reads = 1086206 mem = 536878453 peak = 992481280
[M::process] read 680854 sequences (100000207 bp) in 2.719 CPU sec, 3.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286371, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (323.93, 148.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680742 reads in 175.839 CPU sec, 5.676 real sec
[M::mem_process_seqs] Processed 680998 reads in 116.691 CPU sec, 3.721 real sec
[M::process] read 680502 sequences (100000058 bp) in 2.095 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286732, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (324.30, 147.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 71, #reads = 1084842 mem = 536877873 peak = 992481280
[M::process] read 680514 sequences (100000208 bp) in 2.564 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286784, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (352, 535, 1207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917)
[M::mem_pestat] mean and std.dev: (753.82, 527.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3772)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (324.44, 146.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 680854 reads in 175.960 CPU sec, 5.671 real sec
[M::mem_process_seqs] Processed 680502 reads in 115.252 CPU sec, 3.684 real sec
[M::process] read 679956 sequences (100000158 bp) in 2.511 CPU sec, 2.621 real sec...
sorting block 72, #reads = 1085487 mem = 536878380 peak = 992481280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286978, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (324.94, 146.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679976 sequences (100000250 bp) in 2.548 CPU sec, 2.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285671, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (326.74, 147.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680514 reads in 173.097 CPU sec, 5.620 real sec
sorting block 73, #reads = 1085712 mem = 536881225 peak = 992481280
[M::process] read 679902 sequences (100000068 bp) in 2.452 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286357, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (327.84, 147.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679976 reads in 95.821 CPU sec, 3.070 real sec
[M::process] read 679544 sequences (100000190 bp) in 2.084 CPU sec, 2.256 real sec...
[M::mem_process_seqs] Processed 679956 reads in 258.502 CPU sec, 8.328 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286889, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (328.24, 147.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1085174 mem = 536881232 peak = 992481280
[M::process] read 685512 sequences (100000064 bp) in 3.120 CPU sec, 3.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286437, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (329.81, 147.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679902 reads in 193.213 CPU sec, 6.215 real sec
[M::mem_process_seqs] Processed 679544 reads in 115.787 CPU sec, 3.688 real sec
sorting block 75, #reads = 1083773 mem = 536878034 peak = 1010118656
[M::process] read 686534 sequences (100000005 bp) in 2.535 CPU sec, 2.707 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288318, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.68, 146.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685940 sequences (100000097 bp) in 2.509 CPU sec, 2.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288111, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 2917, 2917)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8277)
[M::mem_pestat] mean and std.dev: (1721.70, 1285.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10957)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 285, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (305.76, 147.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 685512 reads in 190.624 CPU sec, 6.155 real sec
[M::mem_process_seqs] Processed 686534 reads in 123.807 CPU sec, 3.950 real sec
[M::process] read 685876 sequences (100000190 bp) in 1.891 CPU sec, 1.998 real sec...
sorting block 76, #reads = 1083624 mem = 536880279 peak = 1010118656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288210, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (308.67, 147.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 77, #reads = 1085418 mem = 536878207 peak = 1010118656
[M::process] read 685238 sequences (100000298 bp) in 3.068 CPU sec, 3.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288329, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 290, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (309.28, 147.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685940 reads in 191.729 CPU sec, 6.173 real sec
[M::mem_process_seqs] Processed 685876 reads in 124.848 CPU sec, 3.963 real sec
[M::process] read 684368 sequences (100000210 bp) in 2.185 CPU sec, 2.250 real sec...
sorting block 78, #reads = 1086753 mem = 536883945 peak = 1010118656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287762, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 294, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (311.62, 146.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683348 sequences (100000228 bp) in 2.481 CPU sec, 2.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287981, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (314.80, 147.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683496 sequences (100000196 bp) in 1.920 CPU sec, 1.931 real sec...
[M::mem_process_seqs] Processed 685238 reads in 190.109 CPU sec, 6.099 real sec
[M::mem_process_seqs] Processed 684368 reads in 122.365 CPU sec, 3.896 real sec
sorting block 79, #reads = 1083814 mem = 536877246 peak = 1010118656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287323, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (318.43, 146.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683444 sequences (100000024 bp) in 2.708 CPU sec, 3.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287712, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 303, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (318.59, 148.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683348 reads in 184.316 CPU sec, 5.971 real sec
[M::mem_process_seqs] Processed 683496 reads in 118.393 CPU sec, 3.801 real sec
[M::process] read 684898 sequences (100000042 bp) in 2.282 CPU sec, 2.364 real sec...
sorting block 80, #reads = 1082828 mem = 536876956 peak = 1010118656
[M::process] read 685486 sequences (100000087 bp) in 2.533 CPU sec, 2.889 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287705, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (309.95, 144.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685340 sequences (100000042 bp) in 2.002 CPU sec, 2.013 real sec...
[M::mem_process_seqs] Processed 684898 reads in 111.994 CPU sec, 3.542 real sec
sorting block 81, #reads = 1083394 mem = 536880305 peak = 1010118656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287611, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 304, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (318.17, 146.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288740, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.78, 145.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683444 reads in 292.219 CPU sec, 9.383 real sec
[M::mem_process_seqs] Processed 685486 reads in 141.511 CPU sec, 4.547 real sec
[M::process] read 685108 sequences (100000024 bp) in 2.653 CPU sec, 2.777 real sec...
sorting block 82, #reads = 1083952 mem = 536878189 peak = 1010118656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288684, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.06, 144.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684876 sequences (100000283 bp) in 2.867 CPU sec, 3.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288058, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (310.15, 145.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685340 reads in 203.052 CPU sec, 6.536 real sec
[M::process] read 683934 sequences (100000299 bp) in 2.074 CPU sec, 2.092 real sec...
sorting block 83, #reads = 1084037 mem = 536883136 peak = 1010118656
[M::mem_process_seqs] Processed 685108 reads in 131.086 CPU sec, 4.174 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287932, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (311.15, 145.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 504, 854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2004)
[M::mem_pestat] mean and std.dev: (379.22, 222.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2579)
[M::mem_pestat] skip orientation RR
[M::process] read 683136 sequences (100000106 bp) in 2.448 CPU sec, 2.683 real sec...
sorting block 84, #reads = 1082972 mem = 536877009 peak = 1045745664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287771, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (315.47, 147.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 422, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (367.60, 75.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RR
[M::process] read 683150 sequences (100000052 bp) in 2.153 CPU sec, 2.346 real sec...
[M::mem_process_seqs] Processed 684876 reads in 203.569 CPU sec, 6.546 real sec
[M::mem_process_seqs] Processed 683934 reads in 127.376 CPU sec, 4.079 real sec
sorting block 85, #reads = 1081573 mem = 536877591 peak = 1045745664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287471, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 300, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (315.90, 145.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685900 sequences (100000041 bp) in 3.007 CPU sec, 3.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287485, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (317.10, 146.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683136 reads in 192.855 CPU sec, 6.250 real sec
[M::process] read 683294 sequences (100000186 bp) in 2.097 CPU sec, 2.138 real sec...
[M::mem_process_seqs] Processed 683150 reads in 126.197 CPU sec, 4.092 real sec
sorting block 86, #reads = 1082806 mem = 536880525 peak = 1045745664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288312, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (303.60, 140.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683262 sequences (100000055 bp) in 2.380 CPU sec, 2.870 real sec...
[M::process] read 682452 sequences (100000221 bp) in 1.972 CPU sec, 2.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287101, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (313.04, 143.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (388, 388, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (254, 589)
[M::mem_pestat] mean and std.dev: (406.20, 32.43)
[M::mem_pestat] low and high boundaries for proper pairs: (187, 656)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685900 reads in 223.771 CPU sec, 7.235 real sec
[M::mem_process_seqs] Processed 683294 reads in 142.632 CPU sec, 4.583 real sec
sorting block 87, #reads = 1077499 mem = 536878372 peak = 1045745664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287670, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (313.76, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682630 sequences (100000171 bp) in 3.087 CPU sec, 3.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286964, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (317.93, 145.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682400 sequences (100000138 bp) in 2.111 CPU sec, 2.123 real sec...
[M::mem_process_seqs] Processed 683262 reads in 208.699 CPU sec, 6.743 real sec
sorting block 88, #reads = 1077247 mem = 536877922 peak = 1045745664
[M::mem_process_seqs] Processed 682452 reads in 134.455 CPU sec, 4.320 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287767, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (317.24, 145.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 89, #reads = 1079880 mem = 536881240 peak = 1070673920
[M::process] read 682518 sequences (100000171 bp) in 2.804 CPU sec, 3.264 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287404, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (319.42, 147.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 198, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098)
[M::mem_pestat] mean and std.dev: (301.69, 199.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1398)
[M::mem_pestat] skip orientation RR
[M::process] read 682824 sequences (100000008 bp) in 2.098 CPU sec, 2.107 real sec...
[M::mem_process_seqs] Processed 682630 reads in 206.119 CPU sec, 6.622 real sec
[M::mem_process_seqs] Processed 682400 reads in 134.802 CPU sec, 4.294 real sec
sorting block 90, #reads = 1080254 mem = 536878798 peak = 1070673920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287298, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 252, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (257.70, 108.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (317.02, 146.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682242 sequences (100000023 bp) in 3.171 CPU sec, 3.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287721, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 300, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (316.45, 146.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691442 sequences (100000246 bp) in 2.122 CPU sec, 2.132 real sec...
[M::mem_process_seqs] Processed 682518 reads in 208.519 CPU sec, 6.706 real sec
[M::mem_process_seqs] Processed 682824 reads in 136.397 CPU sec, 4.377 real sec
sorting block 91, #reads = 1079689 mem = 536888161 peak = 1070673920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287030, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (319.77, 148.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688414 sequences (100000264 bp) in 2.866 CPU sec, 3.122 real sec...
[M::process] read 686672 sequences (100000279 bp) in 2.050 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290001, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 264, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (285.22, 137.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682242 reads in 215.747 CPU sec, 6.925 real sec
[M::mem_process_seqs] Processed 691442 reads in 146.338 CPU sec, 4.677 real sec
sorting block 92, #reads = 1079456 mem = 536876119 peak = 1070673920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 288817, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 276, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (295.41, 140.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686792 sequences (100000015 bp) in 3.422 CPU sec, 3.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288731, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (302.56, 143.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685468 sequences (100000171 bp) in 2.032 CPU sec, 2.033 real sec...
[M::mem_process_seqs] Processed 688414 reads in 212.828 CPU sec, 6.819 real sec
sorting block 93, #reads = 1076382 mem = 536879152 peak = 1070673920
[M::mem_process_seqs] Processed 686672 reads in 131.062 CPU sec, 4.183 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288932, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 285, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (302.72, 142.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 94, #reads = 1079249 mem = 536878857 peak = 1071685632
[M::process] read 685094 sequences (100000213 bp) in 2.841 CPU sec, 3.255 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288314, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (307.80, 143.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683794 sequences (100000015 bp) in 2.143 CPU sec, 2.198 real sec...
[M::mem_process_seqs] Processed 686792 reads in 214.345 CPU sec, 6.911 real sec
[M::mem_process_seqs] Processed 685468 reads in 134.291 CPU sec, 4.306 real sec
sorting block 95, #reads = 1080508 mem = 536879589 peak = 1071685632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288181, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (310.20, 144.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 250, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (211.89, 72.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 619)
[M::mem_pestat] skip orientation RR
[M::process] read 684158 sequences (100000250 bp) in 3.242 CPU sec, 3.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288247, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (313.57, 144.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685298 sequences (100000096 bp) in 2.107 CPU sec, 2.118 real sec...
[M::mem_process_seqs] Processed 685094 reads in 202.790 CPU sec, 6.494 real sec
[M::mem_process_seqs] Processed 683794 reads in 131.002 CPU sec, 4.173 real sec
sorting block 96, #reads = 1079718 mem = 536879395 peak = 1071685632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287850, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (313.25, 145.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684970 sequences (100000128 bp) in 2.698 CPU sec, 3.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288210, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (307.99, 144.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684158 reads in 194.680 CPU sec, 6.239 real sec
[M::process] read 685114 sequences (100000142 bp) in 2.159 CPU sec, 2.245 real sec...
sorting block 97, #reads = 1081541 mem = 536879721 peak = 1071685632
[M::mem_process_seqs] Processed 685298 reads in 128.214 CPU sec, 4.062 real sec
[M::process] read 684552 sequences (100000005 bp) in 2.650 CPU sec, 3.315 real sec...
sorting block 98, #reads = 1083936 mem = 536878418 peak = 1071685632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 288557, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (478, 478, 860)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1624)
[M::mem_pestat] mean and std.dev: (515.73, 296.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2006)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (309.24, 144.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 685114 reads in 110.856 CPU sec, 3.547 real sec
[M::process] read 684362 sequences (100000152 bp) in 2.252 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288323, 7, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (421, 655, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123)
[M::mem_pestat] mean and std.dev: (538.11, 172.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1357)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (308.88, 144.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287694, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 295, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (311.86, 145.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684970 reads in 297.618 CPU sec, 9.588 real sec
[M::process] read 684216 sequences (100000045 bp) in 2.464 CPU sec, 2.524 real sec...
[M::mem_process_seqs] Processed 684552 reads in 141.169 CPU sec, 4.507 real sec
sorting block 99, #reads = 1083626 mem = 536878311 peak = 1071685632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287803, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (311.41, 145.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683888 sequences (100000143 bp) in 2.858 CPU sec, 3.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288181, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (312.16, 145.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684362 reads in 203.491 CPU sec, 6.553 real sec
sorting block 100, #reads = 1082490 mem = 536877463 peak = 1071685632
[M::process] read 686276 sequences (100000291 bp) in 2.459 CPU sec, 2.522 real sec...
[M::mem_process_seqs] Processed 684216 reads in 132.341 CPU sec, 4.204 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287895, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 298, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (314.83, 147.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 101, #reads = 1081241 mem = 536878513 peak = 1075163136
[M::process] read 682344 sequences (100000043 bp) in 3.043 CPU sec, 3.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288606, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (302.72, 141.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682248 sequences (100000211 bp) in 2.127 CPU sec, 2.130 real sec...
[M::mem_process_seqs] Processed 683888 reads in 218.298 CPU sec, 7.044 real sec
[M::mem_process_seqs] Processed 686276 reads in 151.804 CPU sec, 4.863 real sec
sorting block 102, #reads = 1081332 mem = 536877136 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287233, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 1023, 1440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3968)
[M::mem_pestat] mean and std.dev: (882.30, 762.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5232)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (320.32, 148.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 240, 1456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4012)
[M::mem_pestat] mean and std.dev: (614.67, 633.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5290)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682222 sequences (100000124 bp) in 2.890 CPU sec, 3.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287185, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (322.11, 150.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682344 reads in 196.344 CPU sec, 6.324 real sec
[M::process] read 681938 sequences (100000181 bp) in 2.037 CPU sec, 2.038 real sec...
[M::mem_process_seqs] Processed 682248 reads in 125.695 CPU sec, 4.022 real sec
sorting block 103, #reads = 1076011 mem = 536883264 peak = 1075163136
[M::process] read 681682 sequences (100000258 bp) in 3.101 CPU sec, 3.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287125, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (322.98, 150.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681424 sequences (100000179 bp) in 2.003 CPU sec, 2.008 real sec...
[M::mem_process_seqs] Processed 681938 reads in 110.808 CPU sec, 3.499 real sec
sorting block 104, #reads = 1082873 mem = 536876231 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286911, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (321.08, 149.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (499, 499, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (499, 499)
[M::mem_pestat] mean and std.dev: (499.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (499, 499)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286753, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 215, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (232.38, 95.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1358)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 309, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (327.00, 153.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 682222 reads in 299.989 CPU sec, 9.620 real sec
[M::process] read 681614 sequences (100000057 bp) in 2.244 CPU sec, 2.317 real sec...
sorting block 105, #reads = 1083443 mem = 536880507 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287556, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 309, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (328.16, 154.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681414 sequences (100000243 bp) in 2.169 CPU sec, 2.169 real sec...
[M::mem_process_seqs] Processed 681424 reads in 107.919 CPU sec, 3.685 real sec
[M::mem_process_seqs] Processed 681682 reads in 294.920 CPU sec, 9.737 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286813, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 310, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (328.05, 154.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 106, #reads = 1083096 mem = 536876382 peak = 1075163136
[M::process] read 681874 sequences (100000134 bp) in 2.844 CPU sec, 3.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286696, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (327.24, 154.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681614 reads in 214.131 CPU sec, 6.926 real sec
[M::mem_process_seqs] Processed 681414 reads in 129.522 CPU sec, 4.167 real sec
[M::process] read 687186 sequences (100000145 bp) in 2.736 CPU sec, 2.899 real sec...
sorting block 107, #reads = 1082775 mem = 536878830 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286738, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (324.06, 152.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685354 sequences (100000024 bp) in 2.684 CPU sec, 3.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288453, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 282, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (304.63, 148.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684252 sequences (100000221 bp) in 1.885 CPU sec, 1.986 real sec...
[M::mem_process_seqs] Processed 681874 reads in 218.794 CPU sec, 7.021 real sec
[M::mem_process_seqs] Processed 687186 reads in 135.601 CPU sec, 4.326 real sec
sorting block 108, #reads = 1078332 mem = 536883226 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288044, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 294, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (314.21, 151.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684118 sequences (100000264 bp) in 2.863 CPU sec, 3.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287985, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (316.63, 151.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685354 reads in 182.431 CPU sec, 5.885 real sec
[M::mem_process_seqs] Processed 684252 reads in 116.195 CPU sec, 3.693 real sec
[M::process] read 683460 sequences (100000167 bp) in 2.287 CPU sec, 2.361 real sec...
sorting block 109, #reads = 1080178 mem = 536880484 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288285, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 299, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (316.67, 150.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 110, #reads = 1086637 mem = 536879019 peak = 1075163136
[M::process] read 682384 sequences (100000196 bp) in 3.048 CPU sec, 3.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287754, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 302, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (318.96, 150.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684118 reads in 185.137 CPU sec, 5.970 real sec
[M::mem_process_seqs] Processed 683460 reads in 117.694 CPU sec, 3.748 real sec
[M::process] read 682446 sequences (100000222 bp) in 2.032 CPU sec, 2.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287279, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 231, 911)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2393)
[M::mem_pestat] mean and std.dev: (324.18, 279.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3134)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (322.41, 149.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 111, #reads = 1085507 mem = 536880213 peak = 1075163136
[M::process] read 682106 sequences (100000111 bp) in 3.125 CPU sec, 3.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287448, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 308, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (323.96, 151.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682384 reads in 179.391 CPU sec, 5.836 real sec
[M::mem_process_seqs] Processed 682446 reads in 119.461 CPU sec, 3.827 real sec
[M::process] read 682536 sequences (100000198 bp) in 2.542 CPU sec, 2.726 real sec...
sorting block 112, #reads = 1086243 mem = 536880155 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287025, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 310, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (325.57, 151.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683586 sequences (100000184 bp) in 2.553 CPU sec, 2.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286988, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 309, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (325.35, 152.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682106 reads in 181.259 CPU sec, 5.858 real sec
[M::mem_process_seqs] Processed 682536 reads in 121.487 CPU sec, 3.882 real sec
sorting block 113, #reads = 1084176 mem = 536888592 peak = 1075163136
[M::process] read 685008 sequences (100000275 bp) in 2.555 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287541, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 300, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (317.88, 150.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (422, 422, 1158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2630)
[M::mem_pestat] mean and std.dev: (690.70, 428.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3366)
[M::mem_pestat] skip orientation RR
[M::process] read 683850 sequences (100000212 bp) in 2.611 CPU sec, 2.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288139, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (310.37, 147.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683586 reads in 183.860 CPU sec, 5.903 real sec
[M::mem_process_seqs] Processed 685008 reads in 123.823 CPU sec, 3.934 real sec
sorting block 114, #reads = 1085422 mem = 536879533 peak = 1075163136
[M::process] read 683268 sequences (100000162 bp) in 2.772 CPU sec, 2.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288024, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 300, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (317.46, 150.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 115, #reads = 1085896 mem = 536876851 peak = 1075163136
[M::process] read 682958 sequences (100000018 bp) in 2.597 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287639, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 302, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (320.14, 151.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683850 reads in 180.280 CPU sec, 5.820 real sec
[M::mem_process_seqs] Processed 683268 reads in 118.791 CPU sec, 3.802 real sec
[M::process] read 682130 sequences (100000072 bp) in 2.272 CPU sec, 2.474 real sec...
sorting block 116, #reads = 1086114 mem = 536877645 peak = 1075163136
[M::process] read 681546 sequences (100000154 bp) in 2.735 CPU sec, 3.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287203, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 308, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (325.40, 153.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (429, 707, 707)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263)
[M::mem_pestat] mean and std.dev: (545.91, 259.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1585)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682130 reads in 101.978 CPU sec, 3.234 real sec
[M::process] read 681852 sequences (100000029 bp) in 2.244 CPU sec, 2.303 real sec...
sorting block 117, #reads = 1083045 mem = 536878590 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287581, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 303, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (321.26, 151.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287042, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 309, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (326.39, 152.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (349, 423, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (408.62, 163.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682958 reads in 290.336 CPU sec, 9.341 real sec
[M::mem_process_seqs] Processed 681546 reads in 140.462 CPU sec, 4.480 real sec
[M::process] read 682202 sequences (100000007 bp) in 2.265 CPU sec, 2.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287261, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 309, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (327.14, 153.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 118, #reads = 1084140 mem = 536880029 peak = 1075163136
[M::process] read 682982 sequences (100000138 bp) in 2.993 CPU sec, 3.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287522, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (322.69, 151.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681852 reads in 182.586 CPU sec, 5.907 real sec
[M::mem_process_seqs] Processed 682202 reads in 120.692 CPU sec, 3.871 real sec
[M::process] read 682432 sequences (100000272 bp) in 2.350 CPU sec, 2.538 real sec...
sorting block 119, #reads = 1085486 mem = 536883026 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287229, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (317.95, 149.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682104 sequences (100000166 bp) in 2.567 CPU sec, 3.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287217, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 303, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (322.03, 152.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682982 reads in 195.691 CPU sec, 6.372 real sec
[M::mem_process_seqs] Processed 682432 reads in 124.227 CPU sec, 4.019 real sec
[M::process] read 681970 sequences (100000105 bp) in 2.314 CPU sec, 2.557 real sec...
sorting block 120, #reads = 1082187 mem = 536878652 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287377, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (323.06, 153.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682492 sequences (100000228 bp) in 2.767 CPU sec, 3.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286756, 2, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 259, 2312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6528)
[M::mem_pestat] mean and std.dev: (791.17, 894.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8636)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (323.16, 152.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 682104 reads in 191.951 CPU sec, 6.251 real sec
[M::mem_process_seqs] Processed 681970 reads in 123.032 CPU sec, 3.977 real sec
sorting block 121, #reads = 1082152 mem = 536877862 peak = 1075163136
[M::process] read 682430 sequences (100000087 bp) in 2.235 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287144, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (321.37, 152.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 122, #reads = 1083039 mem = 536886209 peak = 1075163136
[M::process] read 682138 sequences (100000220 bp) in 3.179 CPU sec, 3.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287303, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (322.64, 154.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682492 reads in 194.462 CPU sec, 6.273 real sec
[M::mem_process_seqs] Processed 682430 reads in 122.990 CPU sec, 3.933 real sec
[M::process] read 682852 sequences (100000231 bp) in 2.092 CPU sec, 2.214 real sec...
sorting block 123, #reads = 1082576 mem = 536880489 peak = 1075163136
[M::process] read 682374 sequences (100000001 bp) in 3.096 CPU sec, 3.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287466, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (421, 433, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (397, 457)
[M::mem_pestat] mean and std.dev: (431.00, 4.47)
[M::mem_pestat] low and high boundaries for proper pairs: (385, 469)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 296, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (316.75, 150.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688532 sequences (100000059 bp) in 2.040 CPU sec, 2.067 real sec...
[M::mem_process_seqs] Processed 682852 reads in 113.689 CPU sec, 3.603 real sec
sorting block 124, #reads = 1081935 mem = 536878844 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287223, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (323.37, 153.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286977, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 336, 981)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2423)
[M::mem_pestat] mean and std.dev: (541.10, 379.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3144)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 300, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (320.10, 151.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 682138 reads in 319.748 CPU sec, 10.302 real sec
[M::process] read 686716 sequences (100000086 bp) in 2.235 CPU sec, 2.439 real sec...
[M::mem_process_seqs] Processed 682374 reads in 162.080 CPU sec, 5.262 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289055, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 275, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (298.82, 146.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (335, 421, 1361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3413)
[M::mem_pestat] mean and std.dev: (737.38, 637.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4439)
[M::mem_pestat] skip orientation RR
sorting block 125, #reads = 1079272 mem = 536877642 peak = 1075163136
[M::process] read 685494 sequences (100000007 bp) in 2.930 CPU sec, 3.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288564, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 283, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (305.66, 148.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684498 sequences (100000031 bp) in 2.210 CPU sec, 2.222 real sec...
[M::mem_process_seqs] Processed 688532 reads in 212.140 CPU sec, 6.819 real sec
[M::mem_process_seqs] Processed 686716 reads in 136.774 CPU sec, 4.377 real sec
sorting block 126, #reads = 1077106 mem = 536881610 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288453, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 292, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (312.07, 149.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683350 sequences (100000197 bp) in 3.079 CPU sec, 3.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287681, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 341, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120)
[M::mem_pestat] mean and std.dev: (322.62, 100.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1408)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (316.06, 150.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683688 sequences (100000034 bp) in 1.971 CPU sec, 2.007 real sec...
[M::mem_process_seqs] Processed 685494 reads in 202.993 CPU sec, 6.487 real sec
[M::mem_process_seqs] Processed 684498 reads in 131.284 CPU sec, 4.179 real sec
sorting block 127, #reads = 1081407 mem = 536881737 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287754, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 302, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (319.31, 150.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682958 sequences (100000129 bp) in 3.210 CPU sec, 3.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287996, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 302, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (319.72, 150.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682046 sequences (100000258 bp) in 2.104 CPU sec, 2.116 real sec...
[M::mem_process_seqs] Processed 683350 reads in 203.161 CPU sec, 6.510 real sec
sorting block 128, #reads = 1082211 mem = 536876765 peak = 1075163136
[M::mem_process_seqs] Processed 683688 reads in 132.505 CPU sec, 4.207 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287858, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (323.56, 150.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 129, #reads = 1082207 mem = 536880828 peak = 1075163136
[M::process] read 682642 sequences (100000078 bp) in 3.187 CPU sec, 3.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287505, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (327.50, 152.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683238 sequences (100000075 bp) in 2.044 CPU sec, 2.051 real sec...
[M::mem_process_seqs] Processed 682958 reads in 197.686 CPU sec, 6.325 real sec
[M::mem_process_seqs] Processed 682046 reads in 126.307 CPU sec, 4.008 real sec
sorting block 130, #reads = 1082489 mem = 536880110 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287444, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 308, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (324.02, 151.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682240 sequences (100000110 bp) in 2.994 CPU sec, 3.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287569, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (318.55, 149.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682642 reads in 190.298 CPU sec, 6.117 real sec
[M::process] read 682018 sequences (100000150 bp) in 1.982 CPU sec, 2.108 real sec...
[M::mem_process_seqs] Processed 683238 reads in 123.160 CPU sec, 3.935 real sec
sorting block 131, #reads = 1083371 mem = 536880796 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287518, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (322.82, 149.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681250 sequences (100000219 bp) in 2.999 CPU sec, 3.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287028, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (322.93, 149.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682240 reads in 188.940 CPU sec, 6.092 real sec
[M::process] read 681264 sequences (100000191 bp) in 1.953 CPU sec, 1.984 real sec...
[M::mem_process_seqs] Processed 682018 reads in 123.361 CPU sec, 3.929 real sec
sorting block 132, #reads = 1084193 mem = 536880039 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286952, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 309, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (324.77, 149.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681636 sequences (100000161 bp) in 3.191 CPU sec, 3.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286956, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (487, 669, 1257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2797)
[M::mem_pestat] mean and std.dev: (657.54, 367.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3567)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 308, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (322.28, 146.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 681250 reads in 189.051 CPU sec, 6.085 real sec
sorting block 133, #reads = 1082621 mem = 536879252 peak = 1075163136
[M::mem_process_seqs] Processed 681264 reads in 124.920 CPU sec, 3.959 real sec
[M::process] read 681250 sequences (100000250 bp) in 2.412 CPU sec, 2.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287403, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (318.83, 144.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 134, #reads = 1082598 mem = 536876340 peak = 1075163136
[M::process] read 681220 sequences (100000214 bp) in 3.090 CPU sec, 3.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287104, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (320.95, 145.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681636 reads in 190.223 CPU sec, 6.179 real sec
[M::process] read 681290 sequences (100000189 bp) in 1.892 CPU sec, 2.032 real sec...
[M::mem_process_seqs] Processed 681250 reads in 120.196 CPU sec, 3.885 real sec
sorting block 135, #reads = 1081628 mem = 536881265 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286570, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 305, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (320.00, 145.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681238 sequences (100000299 bp) in 2.905 CPU sec, 3.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286845, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (319.53, 145.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (327, 327, 646)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284)
[M::mem_pestat] mean and std.dev: (479.09, 270.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1603)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681220 reads in 186.949 CPU sec, 6.014 real sec
[M::process] read 680958 sequences (100000271 bp) in 2.084 CPU sec, 2.126 real sec...
[M::mem_process_seqs] Processed 681290 reads in 118.240 CPU sec, 3.772 real sec
sorting block 136, #reads = 1082931 mem = 536878234 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286661, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (319.14, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681210 sequences (100000141 bp) in 2.971 CPU sec, 3.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286705, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 303, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (318.90, 144.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681238 reads in 183.706 CPU sec, 5.926 real sec
[M::process] read 681186 sequences (100000098 bp) in 1.879 CPU sec, 1.950 real sec...
[M::mem_process_seqs] Processed 680958 reads in 117.984 CPU sec, 3.757 real sec
sorting block 137, #reads = 1083725 mem = 536880233 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286166, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 302, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (318.35, 145.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681118 sequences (100000245 bp) in 2.940 CPU sec, 3.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286376, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 301, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (317.53, 144.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681210 reads in 169.135 CPU sec, 5.432 real sec
sorting block 138, #reads = 1083308 mem = 536884308 peak = 1075163136
[M::process] read 680826 sequences (100000190 bp) in 1.560 CPU sec, 1.587 real sec...
[M::mem_process_seqs] Processed 681186 reads in 96.849 CPU sec, 3.060 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286799, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (319.02, 144.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 139, #reads = 1084225 mem = 536879191 peak = 1075163136
[M::process] read 681062 sequences (100000290 bp) in 2.478 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287011, 2, 20)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 305, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (319.92, 145.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (444, 872, 1917)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4863)
[M::mem_pestat] mean and std.dev: (968.85, 643.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6336)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681118 reads in 139.212 CPU sec, 4.423 real sec
[M::mem_process_seqs] Processed 680826 reads in 88.018 CPU sec, 2.766 real sec
[M::process] read 681016 sequences (100000201 bp) in 2.147 CPU sec, 2.210 real sec...
sorting block 140, #reads = 1084203 mem = 536879126 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286814, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (319.78, 145.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681010 sequences (100000050 bp) in 2.938 CPU sec, 3.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286685, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 305, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (321.27, 147.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681062 reads in 165.199 CPU sec, 5.259 real sec
[M::mem_process_seqs] Processed 681016 reads in 111.404 CPU sec, 3.516 real sec
[M::process] read 594008 sequences (87174261 bp) in 2.118 CPU sec, 2.190 real sec...
sorting block 141, #reads = 1084210 mem = 536878400 peak = 1075163136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286213, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (322.10, 147.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 249670, 3, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (262, 262, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (283.60, 69.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (321.38, 148.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 681010 reads in 168.321 CPU sec, 5.494 real sec
[M::mem_process_seqs] Processed 594008 reads in 106.279 CPU sec, 3.448 real sec
sorting block 142, #reads = 1084629 mem = 536878499 peak = 1077583872
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 155415760
overall: 69759012864 mem 24584.854 user 666.407 sys 858.174 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cAd-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:659_cAd-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cAd-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cAd-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cAd-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 858.208 sec; CPU: 25251.264 sec
sorting block 143, #reads = 1003544 mem = 498231659 peak = 1089896448
uniq header count 1
merging 144 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8047146798
output reads: 156012534
bam_mem_sort: 144 calls 105.603 user 2.191 sys 111.105 real
bam_write: 144 calls 90.004 user 5.809 sys 187.603 real
execute: 1 calls 47.463 user 2.888 sys 915.133 real
merge_files: 1 calls 47.439 user 2.864 sys 55.856 real
parse_chunk: 2303 calls 106.866 user 28.990 sys 178.041 real
read_chunk: 6207 calls 55.938 user 33.950 sys 782.895 real
sort_block: 1 calls 39.530 user 32.982 sys 756.786 real
write_chunk: 3902 calls 47.116 user 2.514 sys 54.820 real
overall: 3292467200 mem 2118.909 user 102.085 sys 915.155 real
ls: cannot access '*.cram': No such file or directory