cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_5TB-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1960.057 CPU sec, 78.174 real sec [M::process] read 697750 sequences (100000146 bp) in 1.213 CPU sec, 1.218 real sec... [M::process] read 697586 sequences (100000238 bp) in 1.318 CPU sec, 1.325 real sec... [M::process] read 697200 sequences (100000103 bp) in 1.276 CPU sec, 1.292 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285767, 14, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 255, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092) [M::mem_pestat] mean and std.dev: (328.02, 242.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (269.72, 134.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 880, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3670) [M::mem_pestat] mean and std.dev: (721.31, 702.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (296.84, 221.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696138 sequences (100000168 bp) in 1.591 CPU sec, 1.621 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285335, 12, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 246, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1871) [M::mem_pestat] mean and std.dev: (433.56, 443.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (271.72, 135.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (439, 491, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1912) [M::mem_pestat] mean and std.dev: (489.40, 275.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 244, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1335) [M::mem_pestat] mean and std.dev: (405.44, 239.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1703) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 285414, 22, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 351, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1830) [M::mem_pestat] mean and std.dev: (486.07, 455.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (272.78, 135.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (891, 898, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2577) [M::mem_pestat] mean and std.dev: (911.70, 399.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3139) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 287, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (245.26, 105.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697750 reads in 251.075 CPU sec, 8.127 real sec [M::mem_process_seqs] Processed 697586 reads in 185.965 CPU sec, 6.100 real sec [M::mem_process_seqs] Processed 697200 reads in 203.684 CPU sec, 6.705 real sec sorting block 0, #reads = 1083205 mem = 536882096 peak = 572100608 [M::process] read 695758 sequences (100000034 bp) in 2.406 CPU sec, 2.601 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 285422, 23, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 327, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4701) [M::mem_pestat] mean and std.dev: (899.86, 1273.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (275.32, 136.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (904, 1514, 4574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11914) [M::mem_pestat] mean and std.dev: (2625.70, 1989.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 251, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (274.17, 119.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695708 sequences (100000106 bp) in 1.848 CPU sec, 1.857 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285144, 23, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 282, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3184) [M::mem_pestat] mean and std.dev: (668.16, 808.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (277.66, 138.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 1333, 1390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2908) [M::mem_pestat] mean and std.dev: (1016.25, 583.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3667) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 284, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (306.07, 181.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696138 reads in 174.988 CPU sec, 5.570 real sec sorting block 1, #reads = 1082671 mem = 536887755 peak = 761585664 [M::process] read 695092 sequences (100000068 bp) in 1.699 CPU sec, 1.723 real sec... [M::mem_process_seqs] Processed 695758 reads in 119.573 CPU sec, 4.058 real sec sorting block 2, #reads = 1083081 mem = 536878196 peak = 765415424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285045, 13, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 322, 1744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4882) [M::mem_pestat] mean and std.dev: (811.25, 784.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (278.77, 139.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (962, 1157, 2333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5075) [M::mem_pestat] mean and std.dev: (1520.54, 801.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 359, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832) [M::mem_pestat] mean and std.dev: (439.57, 277.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694864 sequences (100000179 bp) in 2.046 CPU sec, 2.163 real sec... [M::process] read 695534 sequences (100000068 bp) in 1.743 CPU sec, 1.780 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285288, 31, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 571, 3023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8613) [M::mem_pestat] mean and std.dev: (1307.23, 1420.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (279.92, 139.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (549, 615, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2727) [M::mem_pestat] mean and std.dev: (748.88, 618.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 246, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1440) [M::mem_pestat] mean and std.dev: (339.30, 250.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695708 reads in 177.356 CPU sec, 5.725 real sec [M::mem_process_seqs] Processed 695092 reads in 110.159 CPU sec, 3.533 real sec sorting block 3, #reads = 1083040 mem = 536878249 peak = 765415424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284542, 24, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 202, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (328.67, 289.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (279.90, 138.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (528, 879, 6403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18153) [M::mem_pestat] mean and std.dev: (2761.71, 3233.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24028) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 328, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2585) [M::mem_pestat] mean and std.dev: (358.07, 255.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694652 sequences (100000284 bp) in 2.346 CPU sec, 2.475 real sec... [M::process] read 694470 sequences (100000293 bp) in 1.470 CPU sec, 1.472 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285016, 13, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 343, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (320.47, 174.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (279.71, 139.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (411, 870, 2627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7059) [M::mem_pestat] mean and std.dev: (1276.69, 1329.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 265, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2257) [M::mem_pestat] mean and std.dev: (488.88, 547.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694864 reads in 171.215 CPU sec, 5.481 real sec [M::mem_process_seqs] Processed 695534 reads in 108.584 CPU sec, 3.489 real sec sorting block 4, #reads = 1082080 mem = 536877893 peak = 765415424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285022, 22, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 293, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1650) [M::mem_pestat] mean and std.dev: (357.28, 319.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (281.68, 139.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (96, 215, 1324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3780) [M::mem_pestat] mean and std.dev: (630.05, 812.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 272, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1845) [M::mem_pestat] mean and std.dev: (375.24, 287.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694210 sequences (100000019 bp) in 2.562 CPU sec, 2.768 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285045, 10, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 250, 872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2318) [M::mem_pestat] mean and std.dev: (429.31, 478.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (281.98, 139.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (457, 1330, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3823) [M::mem_pestat] mean and std.dev: (968.78, 523.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 330, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (525.26, 503.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694364 sequences (100000276 bp) in 1.559 CPU sec, 1.563 real sec... [M::mem_process_seqs] Processed 694652 reads in 171.066 CPU sec, 5.486 real sec sorting block 5, #reads = 1083036 mem = 536882201 peak = 765415424 [M::mem_process_seqs] Processed 694470 reads in 116.475 CPU sec, 3.726 real sec sorting block 6, #reads = 1083324 mem = 536879864 peak = 765415424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 284604, 24, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 322, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1621) [M::mem_pestat] mean and std.dev: (323.57, 202.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 259, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (282.24, 139.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 473, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3507) [M::mem_pestat] mean and std.dev: (909.75, 831.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 472, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2892) [M::mem_pestat] mean and std.dev: (500.97, 469.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694158 sequences (100000137 bp) in 2.443 CPU sec, 2.596 real sec... [M::process] read 693688 sequences (100000027 bp) in 1.639 CPU sec, 1.635 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285297, 24, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 319, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (378.00, 287.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (283.92, 141.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (254, 665, 1398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3686) [M::mem_pestat] mean and std.dev: (675.85, 632.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4830) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 239, 1757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4983) [M::mem_pestat] mean and std.dev: (667.08, 796.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694210 reads in 178.739 CPU sec, 5.784 real sec [M::mem_process_seqs] Processed 694364 reads in 117.278 CPU sec, 3.800 real sec sorting block 7, #reads = 1082113 mem = 536877411 peak = 765415424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 284908, 11, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 347, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1305) [M::mem_pestat] mean and std.dev: (384.89, 256.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (285.74, 142.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (425, 425, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579) [M::mem_pestat] mean and std.dev: (708.27, 357.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3297) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 348, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1892) [M::mem_pestat] mean and std.dev: (429.66, 340.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695376 sequences (100000194 bp) in 2.217 CPU sec, 2.348 real sec... [M::process] read 695404 sequences (100000074 bp) in 1.515 CPU sec, 1.513 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284693, 37, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 265, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (259.05, 107.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 262, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (286.01, 142.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (825, 1819, 5790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15720) [M::mem_pestat] mean and std.dev: (3231.19, 2691.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 337, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239) [M::mem_pestat] mean and std.dev: (362.51, 257.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694158 reads in 155.253 CPU sec, 4.951 real sec [M::mem_process_seqs] Processed 693688 reads in 98.573 CPU sec, 3.135 real sec sorting block 8, #reads = 1083593 mem = 536881311 peak = 765415424 [M::process] read 694864 sequences (100000234 bp) in 2.216 CPU sec, 2.374 real sec... [M::process] read 694492 sequences (100000065 bp) in 1.459 CPU sec, 1.455 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 285155, 25, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 230, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2346) [M::mem_pestat] mean and std.dev: (496.73, 463.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (279.82, 139.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (574, 1065, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4429) [M::mem_pestat] mean and std.dev: (1212.20, 694.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 525, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2836) [M::mem_pestat] mean and std.dev: (556.03, 401.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695404 reads in 89.430 CPU sec, 2.854 real sec sorting block 9, #reads = 1083120 mem = 536881871 peak = 765415424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 285217, 21, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 519, 774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (503.21, 410.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (280.52, 140.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 852, 2083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5761) [M::mem_pestat] mean and std.dev: (1234.00, 1185.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 298, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349) [M::mem_pestat] mean and std.dev: (342.41, 260.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284834, 28, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 284, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (370.85, 219.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (280.38, 139.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 804, 2739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7459) [M::mem_pestat] mean and std.dev: (1298.81, 1285.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 293, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1586) [M::mem_pestat] mean and std.dev: (314.64, 220.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695376 reads in 240.388 CPU sec, 7.910 real sec [M::process] read 694378 sequences (100000072 bp) in 2.090 CPU sec, 2.127 real sec... [M::mem_process_seqs] Processed 694864 reads in 116.787 CPU sec, 3.731 real sec sorting block 10, #reads = 1083345 mem = 536877463 peak = 765415424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284906, 30, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 309, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270) [M::mem_pestat] mean and std.dev: (308.60, 157.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (281.73, 140.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 560, 2409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6807) [M::mem_pestat] mean and std.dev: (1496.83, 1895.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9079) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 271, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (363.38, 247.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694302 sequences (100000111 bp) in 2.969 CPU sec, 3.169 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284531, 22, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 340, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579) [M::mem_pestat] mean and std.dev: (702.14, 648.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (281.75, 139.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 1662, 2818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7208) [M::mem_pestat] mean and std.dev: (1326.83, 1062.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 358, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691) [M::mem_pestat] mean and std.dev: (402.29, 219.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694492 reads in 156.714 CPU sec, 4.994 real sec [M::mem_process_seqs] Processed 694378 reads in 103.134 CPU sec, 3.260 real sec sorting block 11, #reads = 1082772 mem = 536878675 peak = 765415424 [M::process] read 694288 sequences (100000202 bp) in 2.382 CPU sec, 2.510 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284597, 26, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 224, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (222.84, 120.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (281.72, 139.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (514, 779, 3130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8362) [M::mem_pestat] mean and std.dev: (1721.08, 1818.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 442, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2336) [M::mem_pestat] mean and std.dev: (528.77, 397.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694064 sequences (100000295 bp) in 2.352 CPU sec, 2.473 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284570, 20, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 286, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2006) [M::mem_pestat] mean and std.dev: (434.35, 381.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (281.78, 139.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (543, 578, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11373) [M::mem_pestat] mean and std.dev: (1934.80, 2019.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 277, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (287.51, 110.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693850 sequences (100000096 bp) in 1.570 CPU sec, 1.574 real sec... [M::mem_process_seqs] Processed 694302 reads in 163.091 CPU sec, 5.190 real sec sorting block 12, #reads = 1082247 mem = 536877138 peak = 765415424 [M::mem_process_seqs] Processed 694288 reads in 108.529 CPU sec, 3.436 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284728, 28, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 350, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2019) [M::mem_pestat] mean and std.dev: (399.90, 339.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (281.37, 139.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 698, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3892) [M::mem_pestat] mean and std.dev: (873.08, 781.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 330, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629) [M::mem_pestat] mean and std.dev: (434.24, 331.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 13, #reads = 1082238 mem = 536877142 peak = 765415424 [M::process] read 693212 sequences (100000187 bp) in 2.599 CPU sec, 2.788 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284371, 21, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 301, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (349.37, 270.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (281.69, 139.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 828, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4000) [M::mem_pestat] mean and std.dev: (918.29, 789.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 338, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2683) [M::mem_pestat] mean and std.dev: (558.16, 484.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694064 reads in 156.926 CPU sec, 4.996 real sec [M::process] read 693798 sequences (100000087 bp) in 1.841 CPU sec, 1.875 real sec... [M::mem_process_seqs] Processed 693850 reads in 104.167 CPU sec, 3.290 real sec sorting block 14, #reads = 1081723 mem = 536879559 peak = 765415424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 284242, 20, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 304, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1643) [M::mem_pestat] mean and std.dev: (438.51, 339.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (282.31, 138.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (506, 772, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3338) [M::mem_pestat] mean and std.dev: (767.50, 621.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 322, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (300.26, 136.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693314 sequences (100000300 bp) in 2.607 CPU sec, 2.753 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284601, 12, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 306, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215) [M::mem_pestat] mean and std.dev: (357.14, 238.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (280.14, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 461, 2517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7209) [M::mem_pestat] mean and std.dev: (1064.42, 1218.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 433, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2661) [M::mem_pestat] mean and std.dev: (671.82, 665.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693328 sequences (100000286 bp) in 1.520 CPU sec, 1.526 real sec... [M::mem_process_seqs] Processed 693212 reads in 161.989 CPU sec, 5.158 real sec [M::mem_process_seqs] Processed 693798 reads in 105.757 CPU sec, 3.357 real sec sorting block 15, #reads = 1082322 mem = 536879342 peak = 765415424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284453, 23, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 280, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (285.38, 203.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (280.45, 136.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (129, 656, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2646) [M::mem_pestat] mean and std.dev: (598.91, 478.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 350, 1267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3441) [M::mem_pestat] mean and std.dev: (555.63, 641.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694868 sequences (100000089 bp) in 2.614 CPU sec, 2.786 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 284188, 11, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 271, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1680) [M::mem_pestat] mean and std.dev: (354.77, 298.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (278.88, 135.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1060, 2526, 2526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5458) [M::mem_pestat] mean and std.dev: (1836.20, 900.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6924) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 248, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (355.68, 322.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693314 reads in 162.425 CPU sec, 5.163 real sec [M::process] read 696140 sequences (100000241 bp) in 1.919 CPU sec, 1.951 real sec... [M::mem_process_seqs] Processed 693328 reads in 107.206 CPU sec, 3.478 real sec sorting block 16, #reads = 1082939 mem = 536880664 peak = 765415424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285001, 20, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 342, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1630) [M::mem_pestat] mean and std.dev: (421.33, 341.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (272.87, 131.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (784, 1627, 2321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5395) [M::mem_pestat] mean and std.dev: (1592.05, 917.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 260, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (265.28, 133.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 17, #reads = 1081125 mem = 536879293 peak = 824008704 [M::process] read 693294 sequences (100000275 bp) in 3.112 CPU sec, 3.303 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284826, 25, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 239, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2220) [M::mem_pestat] mean and std.dev: (352.05, 320.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (270.08, 130.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (189, 408, 2525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7197) [M::mem_pestat] mean and std.dev: (1317.65, 1748.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 385, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1359) [M::mem_pestat] mean and std.dev: (375.70, 223.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694868 reads in 178.914 CPU sec, 5.711 real sec [M::mem_process_seqs] Processed 696140 reads in 123.278 CPU sec, 3.893 real sec [M::process] read 693366 sequences (100000128 bp) in 2.201 CPU sec, 2.261 real sec... sorting block 18, #reads = 1076499 mem = 536887045 peak = 824008704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 284142, 27, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (333.42, 223.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 257, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (278.83, 135.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (488, 798, 2094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5306) [M::mem_pestat] mean and std.dev: (1249.00, 1060.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 326, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (329.88, 188.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693266 sequences (100000230 bp) in 2.840 CPU sec, 3.021 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284121, 19, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 250, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (360.44, 215.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (277.85, 134.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 934, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2496) [M::mem_pestat] mean and std.dev: (615.94, 378.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 320, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1751) [M::mem_pestat] mean and std.dev: (385.05, 252.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693266 sequences (100000131 bp) in 1.610 CPU sec, 1.611 real sec... [M::mem_process_seqs] Processed 693294 reads in 167.360 CPU sec, 5.427 real sec [M::mem_process_seqs] Processed 693366 reads in 108.145 CPU sec, 3.418 real sec sorting block 19, #reads = 1078932 mem = 536881499 peak = 824008704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284315, 15, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 564, 2555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7141) [M::mem_pestat] mean and std.dev: (1376.60, 1727.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (277.63, 134.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (137, 369, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3086) [M::mem_pestat] mean and std.dev: (803.40, 904.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 258, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (311.71, 177.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693212 sequences (100000066 bp) in 3.016 CPU sec, 3.232 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284057, 32, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 181, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (225.39, 166.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (278.37, 135.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 553, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4549) [M::mem_pestat] mean and std.dev: (720.13, 739.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 324, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2548) [M::mem_pestat] mean and std.dev: (515.54, 568.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693266 reads in 161.839 CPU sec, 5.152 real sec [M::mem_process_seqs] Processed 693266 reads in 106.440 CPU sec, 3.362 real sec sorting block 20, #reads = 1080821 mem = 536879086 peak = 824008704 [M::process] read 692784 sequences (100000037 bp) in 2.806 CPU sec, 2.960 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 283947, 21, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 323, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1679) [M::mem_pestat] mean and std.dev: (360.72, 246.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (278.05, 135.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (531, 570, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429) [M::mem_pestat] mean and std.dev: (1083.71, 962.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 341, 1515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4121) [M::mem_pestat] mean and std.dev: (953.58, 1124.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692680 sequences (100000043 bp) in 2.807 CPU sec, 2.973 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284253, 21, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 282, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289) [M::mem_pestat] mean and std.dev: (341.89, 256.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 257, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (278.60, 134.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 3134, 5275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15497) [M::mem_pestat] mean and std.dev: (2812.05, 2231.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 303, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (350.55, 243.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693212 reads in 162.928 CPU sec, 5.210 real sec sorting block 21, #reads = 1081832 mem = 536879442 peak = 824008704 [M::mem_process_seqs] Processed 692784 reads in 109.328 CPU sec, 3.450 real sec [M::process] read 692488 sequences (100000269 bp) in 2.342 CPU sec, 2.481 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 283459, 15, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 417, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2734) [M::mem_pestat] mean and std.dev: (654.62, 718.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 258, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (279.50, 135.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1120, 1120, 1937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3571) [M::mem_pestat] mean and std.dev: (1106.00, 682.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 266, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (237.97, 132.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 22, #reads = 1081480 mem = 536882899 peak = 824008704 [M::process] read 692366 sequences (100000086 bp) in 3.116 CPU sec, 3.373 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 283406, 25, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 285, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1610) [M::mem_pestat] mean and std.dev: (314.60, 200.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 259, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (280.41, 135.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (289, 499, 1251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3175) [M::mem_pestat] mean and std.dev: (625.52, 578.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 297, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2132) [M::mem_pestat] mean and std.dev: (470.55, 411.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692680 reads in 175.077 CPU sec, 5.591 real sec [M::mem_process_seqs] Processed 692488 reads in 120.202 CPU sec, 3.818 real sec [M::process] read 692054 sequences (100000092 bp) in 2.751 CPU sec, 2.980 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 284335, 15, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 284, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (279.63, 137.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 260, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (280.79, 135.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (512, 1060, 1922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4742) [M::mem_pestat] mean and std.dev: (1155.07, 965.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 292, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1783) [M::mem_pestat] mean and std.dev: (453.83, 422.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2320) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 23, #reads = 1080273 mem = 536877245 peak = 824008704 [M::process] read 691874 sequences (100000028 bp) in 2.660 CPU sec, 2.970 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 283800, 18, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 277, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (257.49, 127.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 259, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (280.49, 135.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 1141, 5461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15491) [M::mem_pestat] mean and std.dev: (3195.89, 3451.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 248, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (323.91, 194.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692366 reads in 183.805 CPU sec, 5.926 real sec [M::mem_process_seqs] Processed 692054 reads in 122.924 CPU sec, 3.926 real sec sorting block 24, #reads = 1080033 mem = 536877356 peak = 824008704 [M::process] read 692066 sequences (100000030 bp) in 2.458 CPU sec, 2.627 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283653, 15, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 275, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (340.44, 262.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 261, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (281.85, 136.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 559, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2066) [M::mem_pestat] mean and std.dev: (573.07, 427.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2660) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 358, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (345.16, 161.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691808 sequences (100000125 bp) in 2.956 CPU sec, 3.177 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 283947, 22, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 266, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1616) [M::mem_pestat] mean and std.dev: (362.48, 300.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 261, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (280.75, 134.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (283, 1740, 4020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11494) [M::mem_pestat] mean and std.dev: (2376.50, 2675.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15231) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 276, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1304) [M::mem_pestat] mean and std.dev: (319.90, 192.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691874 reads in 184.290 CPU sec, 5.906 real sec [M::mem_process_seqs] Processed 692066 reads in 122.562 CPU sec, 3.883 real sec sorting block 25, #reads = 1080167 mem = 536881374 peak = 836067328 [M::process] read 692172 sequences (100000199 bp) in 2.466 CPU sec, 2.582 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283685, 24, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 213, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (221.34, 125.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 262, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (282.48, 136.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 1781, 5129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14563) [M::mem_pestat] mean and std.dev: (2579.33, 2520.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 265, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (241.79, 85.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 26, #reads = 1081064 mem = 536884469 peak = 870047744 [M::process] read 696822 sequences (100000162 bp) in 3.263 CPU sec, 3.494 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283645, 27, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 202, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (229.88, 136.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 261, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (281.28, 135.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 860, 3425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9743) [M::mem_pestat] mean and std.dev: (2112.63, 2555.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12902) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 616, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4637) [M::mem_pestat] mean and std.dev: (1163.67, 1387.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6714) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691808 reads in 189.240 CPU sec, 6.060 real sec [M::mem_process_seqs] Processed 692172 reads in 127.361 CPU sec, 4.035 real sec [M::process] read 696480 sequences (100000258 bp) in 2.520 CPU sec, 2.662 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285708, 22, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 371, 1650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4368) [M::mem_pestat] mean and std.dev: (1076.28, 1339.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.20, 134.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (810, 1366, 4638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12294) [M::mem_pestat] mean and std.dev: (2210.23, 2059.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16122) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 282, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742) [M::mem_pestat] mean and std.dev: (399.66, 357.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 27, #reads = 1078249 mem = 536882226 peak = 870047744 [M::process] read 695952 sequences (100000193 bp) in 3.031 CPU sec, 3.250 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284768, 12, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 292, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (408.79, 301.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (274.25, 135.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1257, 1830, 1950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3336) [M::mem_pestat] mean and std.dev: (1366.10, 481.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 287, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (313.21, 188.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696822 reads in 186.056 CPU sec, 5.967 real sec [M::mem_process_seqs] Processed 696480 reads in 123.105 CPU sec, 3.901 real sec [M::process] read 695832 sequences (100000224 bp) in 2.396 CPU sec, 2.566 real sec... sorting block 28, #reads = 1081893 mem = 536882400 peak = 870047744 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285431, 19, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 251, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (231.20, 85.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.26, 137.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 1012, 2766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7410) [M::mem_pestat] mean and std.dev: (1860.79, 1936.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 380, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204) [M::mem_pestat] mean and std.dev: (663.51, 804.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695582 sequences (100000085 bp) in 3.118 CPU sec, 3.376 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 285116, 12, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 291, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (318.27, 158.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.55, 137.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (628, 1871, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11203) [M::mem_pestat] mean and std.dev: (2550.17, 2820.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 333, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (278.74, 118.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695952 reads in 187.140 CPU sec, 6.001 real sec [M::mem_process_seqs] Processed 695832 reads in 124.163 CPU sec, 3.936 real sec sorting block 29, #reads = 1084243 mem = 536879055 peak = 870047744 [M::process] read 695270 sequences (100000040 bp) in 2.650 CPU sec, 2.792 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285481, 32, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 282, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (249.95, 139.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (277.82, 138.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 1390, 2539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7253) [M::mem_pestat] mean and std.dev: (1148.71, 969.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 442, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (545.24, 478.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3463) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694968 sequences (100000232 bp) in 3.059 CPU sec, 3.267 real sec... sorting block 30, #reads = 1083917 mem = 536880383 peak = 899923968 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284777, 21, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 337, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144) [M::mem_pestat] mean and std.dev: (475.54, 487.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (280.63, 140.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (316, 646, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3520) [M::mem_pestat] mean and std.dev: (677.83, 492.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 358, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (375.93, 200.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695582 reads in 187.252 CPU sec, 6.033 real sec [M::mem_process_seqs] Processed 695270 reads in 124.766 CPU sec, 3.975 real sec [M::process] read 695082 sequences (100000114 bp) in 2.910 CPU sec, 3.089 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 284986, 12, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 356, 1973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5481) [M::mem_pestat] mean and std.dev: (788.95, 1139.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 257, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (281.28, 140.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (778, 778, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2134) [M::mem_pestat] mean and std.dev: (778.36, 381.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 303, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2672) [M::mem_pestat] mean and std.dev: (596.90, 533.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3500) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 31, #reads = 1084299 mem = 536878921 peak = 899923968 [M::process] read 694704 sequences (100000230 bp) in 2.692 CPU sec, 2.883 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285498, 23, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 277, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (337.38, 192.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 257, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (282.24, 141.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 557, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020) [M::mem_pestat] mean and std.dev: (699.09, 686.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3958) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 383, 1731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4789) [M::mem_pestat] mean and std.dev: (758.59, 774.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694968 reads in 187.696 CPU sec, 6.054 real sec [M::mem_process_seqs] Processed 695082 reads in 123.841 CPU sec, 3.953 real sec [M::process] read 694570 sequences (100000115 bp) in 2.347 CPU sec, 2.492 real sec... sorting block 32, #reads = 1082545 mem = 536876930 peak = 899923968 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 285260, 17, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 304, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1605) [M::mem_pestat] mean and std.dev: (414.45, 355.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (283.33, 141.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 553, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3704) [M::mem_pestat] mean and std.dev: (691.33, 446.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 334, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (316.83, 140.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694314 sequences (100000172 bp) in 2.937 CPU sec, 3.118 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 285114, 46, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 292, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2213) [M::mem_pestat] mean and std.dev: (400.87, 405.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (282.21, 140.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (533, 1306, 2761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7217) [M::mem_pestat] mean and std.dev: (1638.47, 1497.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 558, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1592) [M::mem_pestat] mean and std.dev: (502.52, 295.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694704 reads in 186.273 CPU sec, 5.993 real sec [M::mem_process_seqs] Processed 694570 reads in 123.070 CPU sec, 3.915 real sec sorting block 33, #reads = 1083621 mem = 536879821 peak = 899923968 [M::process] read 694096 sequences (100000241 bp) in 2.985 CPU sec, 3.156 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284950, 27, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1958) [M::mem_pestat] mean and std.dev: (307.40, 291.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 260, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (284.37, 142.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 256, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5648) [M::mem_pestat] mean and std.dev: (1207.76, 1730.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8129) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 314, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300) [M::mem_pestat] mean and std.dev: (316.90, 173.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693542 sequences (100000173 bp) in 2.620 CPU sec, 2.827 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284428, 27, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 254, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (294.09, 224.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (285.19, 142.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (448, 524, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4600) [M::mem_pestat] mean and std.dev: (790.85, 608.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5984) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 414, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2517) [M::mem_pestat] mean and std.dev: (475.80, 411.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694314 reads in 185.393 CPU sec, 5.980 real sec [M::mem_process_seqs] Processed 694096 reads in 123.298 CPU sec, 3.921 real sec sorting block 34, #reads = 1083551 mem = 536877690 peak = 899923968 [M::process] read 693738 sequences (100000240 bp) in 2.777 CPU sec, 2.951 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284634, 8, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 395, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (305.57, 157.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 263, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (287.35, 143.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 280, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (314.69, 156.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 35, #reads = 1083639 mem = 536878956 peak = 899923968 [M::process] read 693316 sequences (100000196 bp) in 2.852 CPU sec, 3.091 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 284941, 13, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 328, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524) [M::mem_pestat] mean and std.dev: (370.27, 291.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (286.74, 143.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (680, 935, 3888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10304) [M::mem_pestat] mean and std.dev: (2387.62, 3058.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14620) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 264, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (295.21, 139.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693542 reads in 187.012 CPU sec, 6.051 real sec [M::mem_process_seqs] Processed 693738 reads in 123.929 CPU sec, 3.952 real sec [M::process] read 693916 sequences (100000133 bp) in 2.839 CPU sec, 3.054 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283890, 13, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 310, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (332.92, 225.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (287.68, 143.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (506, 947, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5048) [M::mem_pestat] mean and std.dev: (1060.92, 749.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 522, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3054) [M::mem_pestat] mean and std.dev: (637.01, 576.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 36, #reads = 1082390 mem = 536878711 peak = 899923968 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285051, 17, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 268, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1496) [M::mem_pestat] mean and std.dev: (361.96, 282.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (284.88, 142.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (96, 543, 1819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5265) [M::mem_pestat] mean and std.dev: (795.53, 805.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 315, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (342.91, 247.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1412) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694392 sequences (100000179 bp) in 3.033 CPU sec, 3.244 real sec... [M::mem_process_seqs] Processed 693316 reads in 187.113 CPU sec, 6.035 real sec [M::mem_process_seqs] Processed 693916 reads in 122.880 CPU sec, 3.916 real sec [M::process] read 694218 sequences (100000026 bp) in 2.912 CPU sec, 3.105 real sec... sorting block 37, #reads = 1082164 mem = 536879529 peak = 899923968 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 285016, 15, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 333, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1591) [M::mem_pestat] mean and std.dev: (333.27, 209.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (281.39, 140.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 665, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2684) [M::mem_pestat] mean and std.dev: (801.87, 782.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 350, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179) [M::mem_pestat] mean and std.dev: (359.38, 190.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284675, 24, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 343, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (353.18, 252.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (282.02, 140.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (501, 830, 1563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3687) [M::mem_pestat] mean and std.dev: (792.52, 550.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 286, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1287) [M::mem_pestat] mean and std.dev: (362.65, 231.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1644) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694538 sequences (100000118 bp) in 3.039 CPU sec, 3.247 real sec... [M::mem_process_seqs] Processed 694392 reads in 189.314 CPU sec, 6.120 real sec [M::mem_process_seqs] Processed 694218 reads in 123.301 CPU sec, 3.949 real sec sorting block 38, #reads = 1082898 mem = 536876581 peak = 899923968 [M::process] read 694078 sequences (100000114 bp) in 3.082 CPU sec, 3.301 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285373, 26, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 280, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2873) [M::mem_pestat] mean and std.dev: (464.16, 476.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (282.73, 141.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 588, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4121) [M::mem_pestat] mean and std.dev: (601.39, 602.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 344, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (331.12, 118.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284781, 21, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 274, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1619) [M::mem_pestat] mean and std.dev: (476.14, 457.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 259, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (282.78, 140.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (326, 527, 6208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17972) [M::mem_pestat] mean and std.dev: (2026.90, 2795.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 314, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1871) [M::mem_pestat] mean and std.dev: (400.23, 375.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694538 reads in 188.288 CPU sec, 6.075 real sec sorting block 39, #reads = 1083369 mem = 536877354 peak = 899923968 [M::process] read 693820 sequences (100000221 bp) in 3.255 CPU sec, 3.504 real sec... [M::mem_process_seqs] Processed 694078 reads in 122.964 CPU sec, 3.935 real sec [M::process] read 693696 sequences (100000042 bp) in 2.853 CPU sec, 3.067 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284652, 18, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 309, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3082) [M::mem_pestat] mean and std.dev: (557.66, 507.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 259, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (282.72, 140.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (410, 534, 1370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3290) [M::mem_pestat] mean and std.dev: (717.00, 532.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 275, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (291.12, 183.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 40, #reads = 1082444 mem = 536879377 peak = 899923968 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285049, 22, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 329, 2502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6988) [M::mem_pestat] mean and std.dev: (1114.67, 1373.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (281.79, 139.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1008, 1167, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (626, 1581) [M::mem_pestat] mean and std.dev: (1138.22, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (435, 1772) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 300, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (437.16, 326.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2320) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693614 sequences (100000172 bp) in 3.001 CPU sec, 3.295 real sec... [M::mem_process_seqs] Processed 693820 reads in 194.343 CPU sec, 6.309 real sec [M::mem_process_seqs] Processed 693696 reads in 129.746 CPU sec, 4.212 real sec [M::process] read 693540 sequences (100000193 bp) in 2.794 CPU sec, 3.030 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284230, 31, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 359, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3900) [M::mem_pestat] mean and std.dev: (737.90, 878.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (280.44, 138.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (363, 971, 1435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3579) [M::mem_pestat] mean and std.dev: (1053.58, 812.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 212, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1446) [M::mem_pestat] mean and std.dev: (344.38, 301.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693614 reads in 108.963 CPU sec, 3.677 real sec sorting block 41, #reads = 1082870 mem = 536881260 peak = 899923968 [M::process] read 693556 sequences (100000059 bp) in 2.904 CPU sec, 3.120 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284147, 14, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 345, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3218) [M::mem_pestat] mean and std.dev: (659.30, 661.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (281.35, 138.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 1333, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4640) [M::mem_pestat] mean and std.dev: (970.67, 924.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6170) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 293, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2733) [M::mem_pestat] mean and std.dev: (574.31, 576.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3574) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 42, #reads = 1082958 mem = 536878005 peak = 899923968 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284313, 23, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 339, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2262) [M::mem_pestat] mean and std.dev: (463.62, 501.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (279.95, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (323, 446, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2339) [M::mem_pestat] mean and std.dev: (633.62, 588.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 380, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (476.37, 374.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693828 sequences (100000106 bp) in 2.990 CPU sec, 3.270 real sec... [M::mem_process_seqs] Processed 693540 reads in 201.229 CPU sec, 6.627 real sec [M::mem_process_seqs] Processed 693556 reads in 126.467 CPU sec, 4.090 real sec [M::process] read 694160 sequences (100000038 bp) in 2.664 CPU sec, 2.866 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284295, 18, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 297, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187) [M::mem_pestat] mean and std.dev: (290.76, 145.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (278.49, 136.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (370, 617, 2130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5650) [M::mem_pestat] mean and std.dev: (1252.67, 1166.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 236, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (259.87, 137.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 43, #reads = 1081488 mem = 536881531 peak = 899923968 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285126, 27, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 312, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (314.62, 161.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (277.78, 136.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 365, 1162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3148) [M::mem_pestat] mean and std.dev: (664.70, 546.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4141) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 341, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1740) [M::mem_pestat] mean and std.dev: (371.37, 255.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694298 sequences (100000225 bp) in 3.086 CPU sec, 3.321 real sec... [M::mem_process_seqs] Processed 693828 reads in 196.623 CPU sec, 6.362 real sec [M::mem_process_seqs] Processed 694160 reads in 129.793 CPU sec, 4.164 real sec sorting block 44, #reads = 1081893 mem = 536878442 peak = 899923968 [M::process] read 694248 sequences (100000290 bp) in 2.961 CPU sec, 3.158 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284629, 14, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 410, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1383) [M::mem_pestat] mean and std.dev: (391.60, 327.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (278.20, 137.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (657, 672, 2161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5169) [M::mem_pestat] mean and std.dev: (1195.64, 919.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 252, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1824) [M::mem_pestat] mean and std.dev: (453.54, 417.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694298 reads in 106.398 CPU sec, 3.535 real sec [M::process] read 697124 sequences (100000139 bp) in 3.200 CPU sec, 3.405 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284961, 23, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 336, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1439) [M::mem_pestat] mean and std.dev: (346.87, 260.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (277.56, 136.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 839, 4431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12405) [M::mem_pestat] mean and std.dev: (1860.83, 1970.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 397, 1239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3321) [M::mem_pestat] mean and std.dev: (690.61, 755.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 45, #reads = 1081308 mem = 536878378 peak = 899923968 [M::process] read 693948 sequences (100000293 bp) in 3.098 CPU sec, 3.340 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285430, 18, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 352, 854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2184) [M::mem_pestat] mean and std.dev: (489.74, 386.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (269.21, 132.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (115, 412, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023) [M::mem_pestat] mean and std.dev: (338.31, 253.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252) [M::mem_pestat] mean and std.dev: (340.20, 237.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1614) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694248 reads in 225.575 CPU sec, 7.317 real sec [M::mem_process_seqs] Processed 697124 reads in 144.039 CPU sec, 4.582 real sec sorting block 46, #reads = 1082280 mem = 536877425 peak = 899923968 [M::process] read 693474 sequences (100000188 bp) in 3.082 CPU sec, 3.294 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284540, 18, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 250, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (322.18, 190.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (277.06, 135.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (236, 607, 1728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4712) [M::mem_pestat] mean and std.dev: (718.73, 683.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 298, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368) [M::mem_pestat] mean and std.dev: (363.68, 249.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693734 sequences (100000026 bp) in 2.923 CPU sec, 3.141 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284493, 17, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 281, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853) [M::mem_pestat] mean and std.dev: (443.60, 357.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (278.51, 136.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (428, 1772, 2780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7484) [M::mem_pestat] mean and std.dev: (1223.00, 992.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 283, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1251) [M::mem_pestat] mean and std.dev: (397.80, 306.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693948 reads in 190.527 CPU sec, 6.122 real sec [M::mem_process_seqs] Processed 693474 reads in 124.010 CPU sec, 3.944 real sec sorting block 47, #reads = 1077158 mem = 536878130 peak = 899923968 [M::process] read 693312 sequences (100000119 bp) in 2.760 CPU sec, 2.936 real sec... sorting block 48, #reads = 1082035 mem = 536877102 peak = 926085120 [M::process] read 693378 sequences (100000147 bp) in 2.983 CPU sec, 3.184 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284327, 22, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 217, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (250.52, 130.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (278.87, 136.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (501, 775, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2664) [M::mem_pestat] mean and std.dev: (790.85, 445.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 261, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206) [M::mem_pestat] mean and std.dev: (321.26, 243.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693312 reads in 105.306 CPU sec, 3.326 real sec [M::process] read 692988 sequences (100000243 bp) in 2.619 CPU sec, 2.765 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284434, 9, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 258, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.88, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (278.15, 136.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 358, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (430.44, 282.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284283, 19, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 349, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2743) [M::mem_pestat] mean and std.dev: (507.25, 458.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (278.56, 135.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (114, 608, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4413) [M::mem_pestat] mean and std.dev: (915.16, 926.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 228, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2064) [M::mem_pestat] mean and std.dev: (538.19, 541.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693734 reads in 290.225 CPU sec, 9.269 real sec [M::mem_process_seqs] Processed 693378 reads in 142.120 CPU sec, 4.514 real sec sorting block 49, #reads = 1082261 mem = 536877693 peak = 926085120 [M::process] read 692918 sequences (100000082 bp) in 2.792 CPU sec, 2.947 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283758, 29, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 252, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4727) [M::mem_pestat] mean and std.dev: (582.76, 691.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (279.76, 136.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1404, 1483, 3287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7053) [M::mem_pestat] mean and std.dev: (2114.03, 1836.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 268, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1740) [M::mem_pestat] mean and std.dev: (315.19, 224.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 50, #reads = 1081142 mem = 536877324 peak = 926085120 [M::process] read 692382 sequences (100000173 bp) in 3.250 CPU sec, 3.473 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 283733, 26, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 300, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3674) [M::mem_pestat] mean and std.dev: (856.15, 1025.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (279.23, 135.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 412, 2544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7278) [M::mem_pestat] mean and std.dev: (1263.08, 1391.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 406, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2506) [M::mem_pestat] mean and std.dev: (640.31, 609.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692988 reads in 186.100 CPU sec, 5.958 real sec [M::mem_process_seqs] Processed 692918 reads in 121.234 CPU sec, 3.839 real sec [M::process] read 692334 sequences (100000045 bp) in 2.668 CPU sec, 2.815 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283888, 21, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 239, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425) [M::mem_pestat] mean and std.dev: (313.43, 222.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 258, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (279.43, 134.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (475, 1021, 6287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17911) [M::mem_pestat] mean and std.dev: (2476.29, 2761.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 291, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2683) [M::mem_pestat] mean and std.dev: (482.27, 452.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 51, #reads = 1080107 mem = 536879126 peak = 926085120 [M::process] read 692236 sequences (100000254 bp) in 3.387 CPU sec, 3.570 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 283968, 12, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 392, 1064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2666) [M::mem_pestat] mean and std.dev: (457.52, 363.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 260, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (281.04, 135.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 809, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2040) [M::mem_pestat] mean and std.dev: (759.75, 366.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 376, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852) [M::mem_pestat] mean and std.dev: (442.31, 259.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692382 reads in 183.769 CPU sec, 5.882 real sec [M::mem_process_seqs] Processed 692334 reads in 120.151 CPU sec, 3.808 real sec [M::process] read 692118 sequences (100000068 bp) in 2.770 CPU sec, 2.889 real sec... sorting block 52, #reads = 1080190 mem = 536888054 peak = 926085120 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 284134, 11, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 306, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2607) [M::mem_pestat] mean and std.dev: (524.97, 546.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 260, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (280.99, 135.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (122.64, 80.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 315, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2550) [M::mem_pestat] mean and std.dev: (478.34, 383.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692078 sequences (100000226 bp) in 2.755 CPU sec, 2.897 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283889, 32, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 309, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (459.64, 411.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 261, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (282.22, 136.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (520, 910, 1840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4480) [M::mem_pestat] mean and std.dev: (1151.75, 1012.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 301, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (303.36, 114.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692236 reads in 177.235 CPU sec, 5.652 real sec [M::mem_process_seqs] Processed 692118 reads in 115.587 CPU sec, 3.647 real sec [M::process] read 691952 sequences (100000150 bp) in 1.913 CPU sec, 1.956 real sec... sorting block 53, #reads = 1080443 mem = 536881852 peak = 926085120 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284355, 13, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 300, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (319.07, 172.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 262, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (283.55, 137.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (549, 850, 2874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7524) [M::mem_pestat] mean and std.dev: (1806.85, 1707.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 285, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1675) [M::mem_pestat] mean and std.dev: (385.98, 277.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2165) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693794 sequences (100000105 bp) in 2.822 CPU sec, 2.986 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 284170, 15, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 393, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2062) [M::mem_pestat] mean and std.dev: (443.56, 367.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 262, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (283.87, 137.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (513, 1429, 3076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8202) [M::mem_pestat] mean and std.dev: (1764.07, 1517.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 286, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3179) [M::mem_pestat] mean and std.dev: (536.54, 576.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4173) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692078 reads in 173.566 CPU sec, 5.535 real sec sorting block 54, #reads = 1080412 mem = 536880727 peak = 926085120 [M::mem_process_seqs] Processed 691952 reads in 113.789 CPU sec, 3.586 real sec [M::process] read 697612 sequences (100000153 bp) in 2.381 CPU sec, 2.448 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284927, 19, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 336, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (360.43, 192.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (281.22, 138.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (189, 384, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4506) [M::mem_pestat] mean and std.dev: (820.78, 1009.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 317, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1704) [M::mem_pestat] mean and std.dev: (363.09, 258.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 55, #reads = 1080369 mem = 536879490 peak = 995360768 [M::process] read 696814 sequences (100000245 bp) in 2.872 CPU sec, 2.986 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285720, 25, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 321, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2481) [M::mem_pestat] mean and std.dev: (704.09, 738.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (272.71, 136.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 541, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3119) [M::mem_pestat] mean and std.dev: (708.12, 704.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4108) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 440, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (465.81, 296.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693794 reads in 177.707 CPU sec, 5.651 real sec [M::process] read 696314 sequences (100000277 bp) in 2.078 CPU sec, 2.086 real sec... [M::mem_process_seqs] Processed 697612 reads in 122.500 CPU sec, 3.880 real sec sorting block 56, #reads = 1082249 mem = 536879399 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 285683, 5, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 241, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (296.82, 221.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.12, 136.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 276, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533) [M::mem_pestat] mean and std.dev: (411.81, 342.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695700 sequences (100000157 bp) in 2.659 CPU sec, 2.822 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 285550, 16, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 224, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (259.79, 162.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.39, 137.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 1198, 1534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3360) [M::mem_pestat] mean and std.dev: (1335.25, 911.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 352, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (383.37, 292.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695506 sequences (100000027 bp) in 1.960 CPU sec, 1.958 real sec... [M::mem_process_seqs] Processed 696814 reads in 172.825 CPU sec, 5.505 real sec [M::mem_process_seqs] Processed 696314 reads in 119.931 CPU sec, 3.820 real sec sorting block 57, #reads = 1083674 mem = 536878987 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285101, 22, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 338, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (460.36, 396.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (277.71, 138.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 1140, 1703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4489) [M::mem_pestat] mean and std.dev: (1203.86, 1064.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5882) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 480, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2669) [M::mem_pestat] mean and std.dev: (609.51, 446.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694986 sequences (100000250 bp) in 2.722 CPU sec, 2.849 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285020, 16, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 358, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (342.74, 183.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (279.06, 139.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 305, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2428) [M::mem_pestat] mean and std.dev: (561.67, 675.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 296, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (291.54, 129.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694392 sequences (100000291 bp) in 1.910 CPU sec, 1.908 real sec... [M::mem_process_seqs] Processed 695700 reads in 170.367 CPU sec, 5.406 real sec [M::mem_process_seqs] Processed 695506 reads in 111.280 CPU sec, 3.513 real sec sorting block 58, #reads = 1082886 mem = 536880917 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285157, 17, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 450, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338) [M::mem_pestat] mean and std.dev: (375.59, 263.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (279.76, 138.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (440, 1483, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4754) [M::mem_pestat] mean and std.dev: (1388.53, 973.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 300, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1804) [M::mem_pestat] mean and std.dev: (326.09, 309.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 59, #reads = 1083402 mem = 536880042 peak = 995360768 [M::process] read 694158 sequences (100000143 bp) in 2.565 CPU sec, 2.693 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285031, 26, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 442, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690) [M::mem_pestat] mean and std.dev: (440.66, 331.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (282.26, 140.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (743, 935, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1958) [M::mem_pestat] mean and std.dev: (806.00, 263.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 373, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2141) [M::mem_pestat] mean and std.dev: (565.67, 511.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693886 sequences (100000138 bp) in 1.960 CPU sec, 1.961 real sec... [M::mem_process_seqs] Processed 694986 reads in 166.546 CPU sec, 5.294 real sec [M::mem_process_seqs] Processed 694392 reads in 108.479 CPU sec, 3.432 real sec sorting block 60, #reads = 1082297 mem = 536878313 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284553, 11, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 232, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (252.58, 117.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (283.59, 140.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 396, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1485) [M::mem_pestat] mean and std.dev: (394.56, 162.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1453) [M::mem_pestat] mean and std.dev: (430.81, 327.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693618 sequences (100000147 bp) in 2.514 CPU sec, 2.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284838, 34, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 501, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4299) [M::mem_pestat] mean and std.dev: (539.88, 548.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (284.41, 141.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (473, 770, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3002) [M::mem_pestat] mean and std.dev: (801.71, 499.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 272, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (332.59, 210.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693120 sequences (100000300 bp) in 1.610 CPU sec, 1.603 real sec... [M::mem_process_seqs] Processed 694158 reads in 166.573 CPU sec, 5.318 real sec [M::mem_process_seqs] Processed 693886 reads in 106.832 CPU sec, 3.383 real sec sorting block 61, #reads = 1082117 mem = 536881340 peak = 995360768 [M::process] read 693030 sequences (100000275 bp) in 2.767 CPU sec, 2.897 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 283981, 29, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 348, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909) [M::mem_pestat] mean and std.dev: (583.20, 567.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 263, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (286.48, 142.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 515, 3156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9252) [M::mem_pestat] mean and std.dev: (1407.55, 1816.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 273, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2007) [M::mem_pestat] mean and std.dev: (463.61, 400.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693120 reads in 89.940 CPU sec, 2.835 real sec [M::process] read 693394 sequences (100000084 bp) in 1.997 CPU sec, 2.006 real sec... sorting block 62, #reads = 1081547 mem = 536880036 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284546, 20, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 221, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (253.48, 127.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 262, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (285.87, 142.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 637, 1440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3724) [M::mem_pestat] mean and std.dev: (904.10, 701.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 369, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2115) [M::mem_pestat] mean and std.dev: (423.14, 341.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284385, 25, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 170, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (339.21, 316.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (288.40, 143.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (338, 338, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1544) [M::mem_pestat] mean and std.dev: (431.35, 201.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1946) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 196, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (235.67, 137.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693618 reads in 252.472 CPU sec, 8.015 real sec [M::process] read 693130 sequences (100000263 bp) in 2.127 CPU sec, 2.154 real sec... [M::mem_process_seqs] Processed 693030 reads in 123.105 CPU sec, 3.898 real sec sorting block 63, #reads = 1081969 mem = 536879225 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 285099, 6, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 359, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (378.12, 192.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (289.25, 145.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 313, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1598) [M::mem_pestat] mean and std.dev: (385.70, 279.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693188 sequences (100000070 bp) in 2.793 CPU sec, 2.971 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 284767, 14, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 318, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533) [M::mem_pestat] mean and std.dev: (377.80, 303.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (289.81, 145.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (682, 3379, 3379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8773) [M::mem_pestat] mean and std.dev: (2125.21, 1297.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 413, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1266) [M::mem_pestat] mean and std.dev: (398.88, 220.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1601) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693394 reads in 158.555 CPU sec, 5.049 real sec [M::process] read 694258 sequences (100000258 bp) in 1.495 CPU sec, 1.497 real sec... [M::mem_process_seqs] Processed 693130 reads in 104.134 CPU sec, 3.298 real sec sorting block 64, #reads = 1082474 mem = 536878581 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 284598, 18, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 289, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (333.20, 230.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (289.14, 144.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (285, 875, 1596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4218) [M::mem_pestat] mean and std.dev: (1081.50, 908.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 436, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355) [M::mem_pestat] mean and std.dev: (397.09, 236.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694806 sequences (100000000 bp) in 2.923 CPU sec, 3.079 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285295, 23, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 389, 1475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3979) [M::mem_pestat] mean and std.dev: (595.86, 565.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 260, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (283.81, 142.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (401, 456, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (585.71, 428.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 325, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2479) [M::mem_pestat] mean and std.dev: (544.37, 474.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693188 reads in 157.822 CPU sec, 5.011 real sec sorting block 65, #reads = 1082842 mem = 536881230 peak = 995360768 [M::mem_process_seqs] Processed 694258 reads in 102.061 CPU sec, 3.215 real sec [M::process] read 694560 sequences (100000005 bp) in 2.102 CPU sec, 2.137 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285005, 19, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 389, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3539) [M::mem_pestat] mean and std.dev: (719.02, 682.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (279.96, 139.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (342, 656, 1452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3672) [M::mem_pestat] mean and std.dev: (914.84, 781.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4782) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 247, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (329.02, 258.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 66, #reads = 1083341 mem = 536878422 peak = 995360768 [M::process] read 694166 sequences (100000004 bp) in 2.748 CPU sec, 2.880 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 284825, 17, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 357, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2578) [M::mem_pestat] mean and std.dev: (542.29, 508.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (280.30, 139.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 717, 2524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6990) [M::mem_pestat] mean and std.dev: (1268.76, 1453.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 344, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1713) [M::mem_pestat] mean and std.dev: (454.28, 304.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694806 reads in 159.256 CPU sec, 5.068 real sec [M::mem_process_seqs] Processed 694560 reads in 103.533 CPU sec, 3.260 real sec [M::process] read 693464 sequences (100000134 bp) in 2.296 CPU sec, 2.355 real sec... sorting block 67, #reads = 1083065 mem = 536878062 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284392, 10, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 239, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (226.57, 116.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (282.43, 140.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 676, 1330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2702) [M::mem_pestat] mean and std.dev: (947.40, 681.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 202, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (278.46, 181.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693698 sequences (100000203 bp) in 2.699 CPU sec, 2.850 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 284574, 30, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 376, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1582) [M::mem_pestat] mean and std.dev: (357.02, 236.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 260, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (283.16, 140.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (326, 615, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4370) [M::mem_pestat] mean and std.dev: (764.11, 661.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5718) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 293, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (248.28, 86.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694166 reads in 160.451 CPU sec, 5.112 real sec [M::mem_process_seqs] Processed 693464 reads in 103.959 CPU sec, 3.276 real sec [M::process] read 693532 sequences (100000002 bp) in 2.264 CPU sec, 2.333 real sec... sorting block 68, #reads = 1082775 mem = 536877911 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284431, 19, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 306, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (288.69, 137.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 259, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (282.26, 139.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 532, 1825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4805) [M::mem_pestat] mean and std.dev: (968.79, 956.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 307, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337) [M::mem_pestat] mean and std.dev: (357.57, 245.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1714) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693328 sequences (100000150 bp) in 2.865 CPU sec, 2.994 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284834, 13, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 241, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (297.46, 267.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (281.26, 138.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 614, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (565.23, 274.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 300, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373) [M::mem_pestat] mean and std.dev: (345.94, 219.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693698 reads in 163.743 CPU sec, 5.216 real sec [M::mem_process_seqs] Processed 693532 reads in 106.521 CPU sec, 3.354 real sec sorting block 69, #reads = 1081274 mem = 536879046 peak = 995360768 [M::process] read 693612 sequences (100000194 bp) in 2.323 CPU sec, 2.392 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284749, 19, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (268.55, 133.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (281.73, 139.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 417, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1540) [M::mem_pestat] mean and std.dev: (329.31, 233.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2017) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 617, 1358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3560) [M::mem_pestat] mean and std.dev: (798.49, 759.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4661) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 70, #reads = 1081667 mem = 536879397 peak = 995360768 [M::process] read 693540 sequences (100000060 bp) in 2.887 CPU sec, 3.015 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284780, 14, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 375, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (404.49, 299.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (281.13, 138.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 651, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (94, 1404) [M::mem_pestat] mean and std.dev: (634.25, 148.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 188, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (203.97, 77.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693328 reads in 162.633 CPU sec, 5.180 real sec [M::mem_process_seqs] Processed 693612 reads in 104.032 CPU sec, 3.283 real sec [M::process] read 693932 sequences (100000047 bp) in 2.173 CPU sec, 2.248 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284166, 21, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 253, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (258.16, 121.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (279.83, 138.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 256, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3464) [M::mem_pestat] mean and std.dev: (504.65, 534.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4605) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 234, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (309.54, 210.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 71, #reads = 1081860 mem = 536879834 peak = 995360768 [M::process] read 693620 sequences (100000184 bp) in 2.513 CPU sec, 2.653 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284403, 22, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 310, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (384.92, 287.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (278.98, 137.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (683, 2941, 4396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11822) [M::mem_pestat] mean and std.dev: (2864.00, 2408.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 254, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (286.87, 143.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693540 reads in 164.864 CPU sec, 5.261 real sec [M::process] read 693864 sequences (100000275 bp) in 1.613 CPU sec, 1.623 real sec... [M::mem_process_seqs] Processed 693932 reads in 114.036 CPU sec, 3.621 real sec sorting block 72, #reads = 1080608 mem = 536878125 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284432, 10, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 278, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2018) [M::mem_pestat] mean and std.dev: (382.88, 334.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (279.34, 137.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (203, 783, 1386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3752) [M::mem_pestat] mean and std.dev: (857.60, 778.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 364, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (402.41, 289.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2166) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694794 sequences (100000287 bp) in 2.822 CPU sec, 2.981 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284566, 23, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 229, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (249.72, 147.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (278.83, 137.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 845, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3728) [M::mem_pestat] mean and std.dev: (1026.64, 1026.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 348, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (340.92, 168.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693620 reads in 162.421 CPU sec, 5.165 real sec [M::mem_process_seqs] Processed 693864 reads in 104.111 CPU sec, 3.275 real sec [M::process] read 693990 sequences (100000101 bp) in 2.255 CPU sec, 2.311 real sec... sorting block 73, #reads = 1082013 mem = 536878954 peak = 995360768 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284671, 14, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 329, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1481) [M::mem_pestat] mean and std.dev: (398.49, 255.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 252, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (275.76, 135.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (239, 284, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2735) [M::mem_pestat] mean and std.dev: (432.83, 508.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 330, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1500) [M::mem_pestat] mean and std.dev: (380.38, 215.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693704 sequences (100000233 bp) in 2.877 CPU sec, 2.994 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284398, 20, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 400, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1975) [M::mem_pestat] mean and std.dev: (507.86, 372.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (278.03, 136.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (482, 617, 1769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4343) [M::mem_pestat] mean and std.dev: (924.25, 720.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 268, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (363.08, 313.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694794 reads in 167.831 CPU sec, 5.338 real sec sorting block 74, #reads = 1080931 mem = 536879589 peak = 995360768 [M::mem_process_seqs] Processed 693990 reads in 106.777 CPU sec, 3.359 real sec [M::process] read 693642 sequences (100000042 bp) in 2.180 CPU sec, 2.205 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284090, 13, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 342, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1487) [M::mem_pestat] mean and std.dev: (356.65, 262.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (279.38, 137.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 480, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3792) [M::mem_pestat] mean and std.dev: (618.62, 626.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5035) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 303, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300) [M::mem_pestat] mean and std.dev: (345.30, 231.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 75, #reads = 1079814 mem = 536876584 peak = 1025495040 [M::process] read 693598 sequences (100000279 bp) in 2.713 CPU sec, 2.859 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284274, 22, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 220, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (335.28, 271.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (279.70, 137.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (340, 1277, 2356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6388) [M::mem_pestat] mean and std.dev: (1354.10, 1372.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 324, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1464) [M::mem_pestat] mean and std.dev: (413.04, 340.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693704 reads in 165.056 CPU sec, 5.263 real sec [M::mem_process_seqs] Processed 693642 reads in 106.272 CPU sec, 3.346 real sec [M::process] read 693504 sequences (100000006 bp) in 2.244 CPU sec, 2.301 real sec... sorting block 76, #reads = 1080705 mem = 536882053 peak = 1025495040 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284664, 20, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 332, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361) [M::mem_pestat] mean and std.dev: (358.25, 247.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (280.25, 138.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 680, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2471) [M::mem_pestat] mean and std.dev: (666.44, 410.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3146) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 320, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (314.77, 131.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693084 sequences (100000210 bp) in 2.762 CPU sec, 2.894 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284253, 16, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 460, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279) [M::mem_pestat] mean and std.dev: (719.96, 661.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (279.89, 137.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 456, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2435) [M::mem_pestat] mean and std.dev: (489.86, 566.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 283, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (286.85, 164.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693598 reads in 161.828 CPU sec, 5.154 real sec [M::process] read 693026 sequences (100000095 bp) in 1.485 CPU sec, 1.493 real sec... [M::mem_process_seqs] Processed 693504 reads in 105.587 CPU sec, 3.338 real sec sorting block 77, #reads = 1080947 mem = 536878852 peak = 1025495040 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284558, 19, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 359, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2396) [M::mem_pestat] mean and std.dev: (504.24, 413.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (280.54, 137.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 1048, 3386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9410) [M::mem_pestat] mean and std.dev: (1544.26, 1429.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 246, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (293.02, 216.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692752 sequences (100000037 bp) in 3.115 CPU sec, 3.279 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 284040, 18, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 286, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (424.37, 366.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (280.53, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (88, 1046, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5749) [M::mem_pestat] mean and std.dev: (1002.00, 889.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 364, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1938) [M::mem_pestat] mean and std.dev: (580.59, 576.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693084 reads in 160.686 CPU sec, 5.100 real sec [M::mem_process_seqs] Processed 693026 reads in 103.722 CPU sec, 3.268 real sec [M::process] read 692774 sequences (100000071 bp) in 2.148 CPU sec, 2.195 real sec... sorting block 78, #reads = 1082365 mem = 536878457 peak = 1025495040 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284415, 26, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 354, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (327.27, 149.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (281.09, 137.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (437, 1698, 3412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9362) [M::mem_pestat] mean and std.dev: (2060.42, 2066.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 453, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2610) [M::mem_pestat] mean and std.dev: (592.92, 613.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 79, #reads = 1081252 mem = 536881827 peak = 1025495040 [M::process] read 692492 sequences (100000099 bp) in 2.260 CPU sec, 2.368 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284216, 29, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 369, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (341.64, 176.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 259, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (281.80, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (142, 467, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2734) [M::mem_pestat] mean and std.dev: (485.08, 397.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 282, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739) [M::mem_pestat] mean and std.dev: (417.55, 398.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692336 sequences (100000134 bp) in 1.460 CPU sec, 1.464 real sec... [M::mem_process_seqs] Processed 692752 reads in 160.349 CPU sec, 5.119 real sec [M::mem_process_seqs] Processed 692774 reads in 103.254 CPU sec, 3.269 real sec sorting block 80, #reads = 1081091 mem = 536879417 peak = 1025495040 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 283998, 18, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 279, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1662) [M::mem_pestat] mean and std.dev: (329.46, 248.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 260, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (282.09, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 426, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2147) [M::mem_pestat] mean and std.dev: (482.27, 333.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 244, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217) [M::mem_pestat] mean and std.dev: (256.75, 134.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693114 sequences (100000079 bp) in 2.684 CPU sec, 2.838 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 284088, 20, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 223, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (251.29, 118.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 260, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (282.87, 138.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 591, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3499) [M::mem_pestat] mean and std.dev: (698.89, 750.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 379, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2595) [M::mem_pestat] mean and std.dev: (595.64, 595.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692108 sequences (100000011 bp) in 1.480 CPU sec, 1.479 real sec... [M::mem_process_seqs] Processed 692492 reads in 164.680 CPU sec, 5.250 real sec [M::mem_process_seqs] Processed 692336 reads in 107.753 CPU sec, 3.414 real sec sorting block 81, #reads = 1080994 mem = 536877346 peak = 1025495040 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283824, 11, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 329, 1649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4447) [M::mem_pestat] mean and std.dev: (1071.78, 1353.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (281.39, 138.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 272, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7283) [M::mem_pestat] mean and std.dev: (942.64, 1195.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9620) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 241, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998) [M::mem_pestat] mean and std.dev: (229.30, 101.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691702 sequences (100000134 bp) in 2.456 CPU sec, 2.596 real sec... [M::process] read 694056 sequences (100000060 bp) in 1.510 CPU sec, 1.503 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 283749, 28, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 300, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985) [M::mem_pestat] mean and std.dev: (437.06, 442.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 261, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (282.93, 138.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (577, 1065, 1937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4657) [M::mem_pestat] mean and std.dev: (1250.54, 848.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6017) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 275, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (413.05, 353.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693114 reads in 163.031 CPU sec, 5.199 real sec [M::mem_process_seqs] Processed 692108 reads in 105.122 CPU sec, 3.348 real sec sorting block 82, #reads = 1080203 mem = 536879151 peak = 1025495040 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 283706, 21, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 272, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (392.54, 358.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 263, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (284.78, 138.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (721, 1148, 2061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4741) [M::mem_pestat] mean and std.dev: (950.61, 629.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6081) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 287, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (274.78, 137.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696400 sequences (100000231 bp) in 2.570 CPU sec, 2.692 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284351, 13, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 295, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247) [M::mem_pestat] mean and std.dev: (463.41, 388.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (277.93, 136.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 557, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2515) [M::mem_pestat] mean and std.dev: (437.36, 335.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 331, 1380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3796) [M::mem_pestat] mean and std.dev: (898.82, 1092.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691702 reads in 164.616 CPU sec, 5.234 real sec [M::process] read 695932 sequences (100000105 bp) in 1.890 CPU sec, 1.888 real sec... sorting block 83, #reads = 1079936 mem = 536875910 peak = 1025495040 [M::mem_process_seqs] Processed 694056 reads in 107.629 CPU sec, 3.401 real sec sorting block 84, #reads = 1079893 mem = 536878117 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 285565, 16, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 306, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (341.76, 252.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.22, 136.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 695, 2365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6955) [M::mem_pestat] mean and std.dev: (573.85, 677.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 237, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221) [M::mem_pestat] mean and std.dev: (314.43, 218.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1566) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695344 sequences (100000135 bp) in 2.891 CPU sec, 3.026 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285305, 23, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 316, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (263.48, 138.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (276.11, 137.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 895, 2429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6687) [M::mem_pestat] mean and std.dev: (1269.26, 1236.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 288, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270) [M::mem_pestat] mean and std.dev: (339.02, 203.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696400 reads in 160.599 CPU sec, 5.099 real sec [M::process] read 695178 sequences (100000075 bp) in 1.984 CPU sec, 1.997 real sec... [M::mem_process_seqs] Processed 695932 reads in 109.095 CPU sec, 3.456 real sec sorting block 85, #reads = 1083476 mem = 536880403 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285504, 15, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 666, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2405) [M::mem_pestat] mean and std.dev: (686.86, 635.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (279.15, 139.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 214, 2580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7416) [M::mem_pestat] mean and std.dev: (1302.27, 1703.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 323, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (335.50, 196.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694826 sequences (100000064 bp) in 2.718 CPU sec, 2.851 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285144, 21, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 285, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (284.02, 150.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (279.52, 140.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 661, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264) [M::mem_pestat] mean and std.dev: (674.90, 591.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 389, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3900) [M::mem_pestat] mean and std.dev: (854.98, 880.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695344 reads in 159.712 CPU sec, 5.089 real sec [M::process] read 694532 sequences (100000205 bp) in 1.764 CPU sec, 1.781 real sec... [M::mem_process_seqs] Processed 695178 reads in 105.870 CPU sec, 3.333 real sec sorting block 86, #reads = 1084296 mem = 536880403 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285064, 26, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 306, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1620) [M::mem_pestat] mean and std.dev: (346.14, 236.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (281.39, 141.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1039, 1045, 2824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6394) [M::mem_pestat] mean and std.dev: (1779.85, 1550.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 446, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3144) [M::mem_pestat] mean and std.dev: (672.60, 651.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694448 sequences (100000296 bp) in 2.782 CPU sec, 2.924 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284642, 11, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 252, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2856) [M::mem_pestat] mean and std.dev: (729.24, 884.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (282.42, 141.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 982, 3450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9638) [M::mem_pestat] mean and std.dev: (1694.27, 1525.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 282, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (427.97, 374.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694826 reads in 158.003 CPU sec, 5.041 real sec [M::mem_process_seqs] Processed 694532 reads in 102.797 CPU sec, 3.245 real sec sorting block 87, #reads = 1083679 mem = 536877757 peak = 1091633152 [M::process] read 694040 sequences (100000169 bp) in 2.026 CPU sec, 2.089 real sec... sorting block 88, #reads = 1084504 mem = 536880353 peak = 1091633152 [M::process] read 693824 sequences (100000041 bp) in 2.677 CPU sec, 2.830 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284000, 14, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 345, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800) [M::mem_pestat] mean and std.dev: (474.98, 484.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 259, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (284.39, 142.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (249, 1175, 4799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13899) [M::mem_pestat] mean and std.dev: (2139.71, 1995.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 294, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (396.14, 297.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693832 sequences (100000001 bp) in 1.450 CPU sec, 1.445 real sec... [M::mem_process_seqs] Processed 694040 reads in 95.130 CPU sec, 3.005 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284870, 13, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 197, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (196.15, 91.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (284.18, 142.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 827, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12296) [M::mem_pestat] mean and std.dev: (2105.31, 3112.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16390) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 597, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2319) [M::mem_pestat] mean and std.dev: (601.58, 421.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 284349, 16, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 429, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1971) [M::mem_pestat] mean and std.dev: (471.91, 364.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (285.18, 142.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (496, 1198, 4823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13477) [M::mem_pestat] mean and std.dev: (2296.81, 2744.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 345, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1415) [M::mem_pestat] mean and std.dev: (351.03, 207.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694448 reads in 251.298 CPU sec, 7.989 real sec [M::process] read 692786 sequences (100000150 bp) in 2.388 CPU sec, 2.415 real sec... [M::mem_process_seqs] Processed 693824 reads in 121.256 CPU sec, 3.848 real sec sorting block 89, #reads = 1083309 mem = 536878225 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285140, 12, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 262, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (256.56, 144.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 260, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (285.09, 142.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (183, 847, 3500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10134) [M::mem_pestat] mean and std.dev: (1533.67, 1934.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 578, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2001) [M::mem_pestat] mean and std.dev: (469.45, 297.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 90, #reads = 1081816 mem = 536876944 peak = 1091633152 [M::process] read 692534 sequences (100000218 bp) in 2.822 CPU sec, 3.013 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284189, 26, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 346, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1745) [M::mem_pestat] mean and std.dev: (381.30, 289.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 265, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (288.71, 143.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 229, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1699) [M::mem_pestat] mean and std.dev: (402.84, 373.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 304, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2674) [M::mem_pestat] mean and std.dev: (484.70, 461.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693832 reads in 159.610 CPU sec, 5.081 real sec [M::mem_process_seqs] Processed 692786 reads in 104.676 CPU sec, 3.300 real sec [M::process] read 692196 sequences (100000217 bp) in 2.189 CPU sec, 2.261 real sec... sorting block 91, #reads = 1083588 mem = 536879091 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 283545, 15, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 278, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (287.67, 118.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 265, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (288.81, 143.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 645, 2435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6715) [M::mem_pestat] mean and std.dev: (1255.53, 1238.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 288, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3326) [M::mem_pestat] mean and std.dev: (482.91, 421.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692246 sequences (100000105 bp) in 2.807 CPU sec, 2.946 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284001, 18, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 245, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2350) [M::mem_pestat] mean and std.dev: (406.40, 393.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 266, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (290.01, 144.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (323, 988, 2104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5666) [M::mem_pestat] mean and std.dev: (1447.89, 1544.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 309, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2394) [M::mem_pestat] mean and std.dev: (428.00, 435.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692534 reads in 159.684 CPU sec, 5.072 real sec [M::process] read 692074 sequences (100000184 bp) in 1.862 CPU sec, 1.892 real sec... [M::mem_process_seqs] Processed 692196 reads in 104.102 CPU sec, 3.277 real sec sorting block 92, #reads = 1081541 mem = 536878924 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283939, 26, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 333, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (316.00, 173.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (291.46, 145.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (142, 597, 2407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6937) [M::mem_pestat] mean and std.dev: (1111.80, 1061.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 283, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (357.13, 258.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693940 sequences (100000027 bp) in 2.989 CPU sec, 3.162 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 283841, 31, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 262, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (273.04, 140.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 267, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (291.87, 145.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (696, 1190, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6426) [M::mem_pestat] mean and std.dev: (1809.68, 1502.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 442, 1634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4526) [M::mem_pestat] mean and std.dev: (793.70, 762.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692246 reads in 158.898 CPU sec, 5.056 real sec [M::mem_process_seqs] Processed 692074 reads in 103.739 CPU sec, 3.268 real sec [M::process] read 694306 sequences (100000133 bp) in 2.291 CPU sec, 2.363 real sec... sorting block 93, #reads = 1082467 mem = 536879722 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 284513, 21, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 233, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4037) [M::mem_pestat] mean and std.dev: (732.46, 800.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (283.98, 141.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (614, 818, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3857) [M::mem_pestat] mean and std.dev: (1180.05, 1036.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5324) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 269, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629) [M::mem_pestat] mean and std.dev: (359.69, 292.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694548 sequences (100000117 bp) in 2.817 CPU sec, 2.953 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284336, 8, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 347, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (342.58, 229.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (282.18, 141.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 262, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (303.37, 151.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693940 reads in 160.792 CPU sec, 5.127 real sec sorting block 94, #reads = 1081412 mem = 536878871 peak = 1091633152 [M::mem_process_seqs] Processed 694306 reads in 105.773 CPU sec, 3.343 real sec [M::process] read 693512 sequences (100000107 bp) in 2.310 CPU sec, 2.390 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 284617, 15, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 360, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2188) [M::mem_pestat] mean and std.dev: (449.78, 437.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 257, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (281.81, 141.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 358, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629) [M::mem_pestat] mean and std.dev: (490.93, 341.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 246, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1287) [M::mem_pestat] mean and std.dev: (338.13, 270.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 95, #reads = 1081952 mem = 536884213 peak = 1091633152 [M::process] read 693600 sequences (100000042 bp) in 2.673 CPU sec, 2.822 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 284190, 34, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 246, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1500) [M::mem_pestat] mean and std.dev: (327.69, 262.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 259, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (284.19, 141.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (419, 661, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2570) [M::mem_pestat] mean and std.dev: (759.19, 516.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 254, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (328.23, 201.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1629) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694548 reads in 160.471 CPU sec, 5.118 real sec [M::mem_process_seqs] Processed 693512 reads in 105.337 CPU sec, 3.329 real sec [M::process] read 693668 sequences (100000197 bp) in 2.405 CPU sec, 2.489 real sec... sorting block 96, #reads = 1082617 mem = 536880736 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284249, 16, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 312, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1293) [M::mem_pestat] mean and std.dev: (369.29, 232.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (283.07, 141.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (396, 3145, 7283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21057) [M::mem_pestat] mean and std.dev: (3234.94, 2999.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 27944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 425, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1662) [M::mem_pestat] mean and std.dev: (475.61, 360.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693538 sequences (100000040 bp) in 2.548 CPU sec, 2.681 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 284427, 19, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 266, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1601) [M::mem_pestat] mean and std.dev: (361.93, 306.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (281.98, 140.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (573, 1084, 2717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7005) [M::mem_pestat] mean and std.dev: (1614.89, 1475.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 320, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1670) [M::mem_pestat] mean and std.dev: (470.37, 391.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693600 reads in 160.853 CPU sec, 5.128 real sec [M::mem_process_seqs] Processed 693668 reads in 104.284 CPU sec, 3.288 real sec [M::process] read 693538 sequences (100000097 bp) in 2.059 CPU sec, 2.136 real sec... sorting block 97, #reads = 1082423 mem = 536878264 peak = 1091633152 [M::process] read 693644 sequences (100000032 bp) in 2.697 CPU sec, 2.828 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 284345, 20, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 452, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1530) [M::mem_pestat] mean and std.dev: (461.74, 315.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (281.32, 138.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 982, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3054) [M::mem_pestat] mean and std.dev: (833.84, 700.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3998) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 289, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (294.94, 139.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693538 reads in 84.500 CPU sec, 2.654 real sec [M::process] read 693650 sequences (100000064 bp) in 1.649 CPU sec, 1.689 real sec... sorting block 98, #reads = 1082011 mem = 536888080 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 284235, 17, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 308, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (447.85, 402.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (282.35, 139.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (253, 340, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1519) [M::mem_pestat] mean and std.dev: (419.94, 316.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 304, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419) [M::mem_pestat] mean and std.dev: (368.19, 276.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284483, 23, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 264, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (339.63, 284.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (280.66, 138.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 550, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3041) [M::mem_pestat] mean and std.dev: (641.00, 553.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 349, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2198) [M::mem_pestat] mean and std.dev: (561.17, 550.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693538 reads in 234.200 CPU sec, 7.438 real sec [M::process] read 693516 sequences (100000036 bp) in 1.910 CPU sec, 1.953 real sec... [M::mem_process_seqs] Processed 693644 reads in 106.699 CPU sec, 3.377 real sec sorting block 99, #reads = 1081923 mem = 536881193 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284547, 27, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 280, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173) [M::mem_pestat] mean and std.dev: (331.06, 237.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (279.41, 138.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (858, 960, 1860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3864) [M::mem_pestat] mean and std.dev: (1226.62, 882.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 241, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (319.45, 231.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693600 sequences (100000060 bp) in 2.543 CPU sec, 2.729 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 284261, 12, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 389, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1512) [M::mem_pestat] mean and std.dev: (454.55, 331.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (279.83, 137.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 314, 2671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7685) [M::mem_pestat] mean and std.dev: (1085.17, 1166.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 274, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (273.09, 153.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693650 reads in 141.212 CPU sec, 4.495 real sec [M::mem_process_seqs] Processed 693516 reads in 92.509 CPU sec, 2.921 real sec [M::process] read 693554 sequences (100000289 bp) in 1.905 CPU sec, 1.958 real sec... sorting block 100, #reads = 1081678 mem = 536877419 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283837, 30, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 394, 1374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3632) [M::mem_pestat] mean and std.dev: (793.41, 793.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (279.34, 137.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 1000, 4232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12026) [M::mem_pestat] mean and std.dev: (2363.97, 2456.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 300, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (307.57, 157.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 101, #reads = 1081329 mem = 536877781 peak = 1091633152 [M::process] read 693408 sequences (100000140 bp) in 2.891 CPU sec, 3.064 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284427, 21, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 257, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (274.85, 130.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (279.59, 137.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 602, 2577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7309) [M::mem_pestat] mean and std.dev: (1363.43, 1548.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 403, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (370.17, 180.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693600 reads in 156.235 CPU sec, 4.971 real sec [M::mem_process_seqs] Processed 693554 reads in 102.117 CPU sec, 3.227 real sec [M::process] read 693168 sequences (100000156 bp) in 2.043 CPU sec, 2.111 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 284378, 24, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1820) [M::mem_pestat] mean and std.dev: (430.91, 420.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (280.44, 137.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 374, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1670) [M::mem_pestat] mean and std.dev: (427.46, 271.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 332, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (289.07, 130.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 102, #reads = 1081508 mem = 536878372 peak = 1091633152 [M::process] read 693330 sequences (100000157 bp) in 2.333 CPU sec, 2.496 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 284308, 20, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 317, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623) [M::mem_pestat] mean and std.dev: (417.02, 332.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (281.48, 137.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 1068, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2586) [M::mem_pestat] mean and std.dev: (798.37, 519.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 244, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455) [M::mem_pestat] mean and std.dev: (315.12, 297.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693408 reads in 159.076 CPU sec, 5.081 real sec [M::process] read 693442 sequences (100000146 bp) in 1.527 CPU sec, 1.527 real sec... [M::mem_process_seqs] Processed 693168 reads in 105.420 CPU sec, 3.337 real sec sorting block 103, #reads = 1082974 mem = 536879033 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284672, 23, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 272, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2476) [M::mem_pestat] mean and std.dev: (526.70, 518.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (280.81, 138.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (464, 760, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4274) [M::mem_pestat] mean and std.dev: (1154.95, 999.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 356, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1813) [M::mem_pestat] mean and std.dev: (467.88, 347.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693332 sequences (100000161 bp) in 2.740 CPU sec, 2.886 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283769, 26, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 282, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (382.96, 325.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (281.13, 139.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (524, 1705, 2029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5039) [M::mem_pestat] mean and std.dev: (1388.12, 966.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 290, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (286.26, 135.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693330 reads in 159.685 CPU sec, 5.079 real sec [M::mem_process_seqs] Processed 693442 reads in 104.163 CPU sec, 3.284 real sec [M::process] read 693410 sequences (100000131 bp) in 2.252 CPU sec, 2.313 real sec... sorting block 104, #reads = 1080917 mem = 536877035 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284262, 22, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 287, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (281.66, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (280.49, 138.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (103, 548, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2869) [M::mem_pestat] mean and std.dev: (516.74, 593.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 300, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (299.69, 145.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693344 sequences (100000087 bp) in 2.804 CPU sec, 2.942 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 284164, 18, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 373, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3162) [M::mem_pestat] mean and std.dev: (743.05, 835.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (280.56, 138.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 794, 1798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4904) [M::mem_pestat] mean and std.dev: (897.78, 692.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 324, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1964) [M::mem_pestat] mean and std.dev: (498.22, 515.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693332 reads in 157.446 CPU sec, 5.007 real sec [M::mem_process_seqs] Processed 693410 reads in 99.499 CPU sec, 3.130 real sec sorting block 105, #reads = 1081847 mem = 536883773 peak = 1091633152 [M::process] read 692596 sequences (100000019 bp) in 2.215 CPU sec, 2.274 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284390, 14, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 318, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422) [M::mem_pestat] mean and std.dev: (545.84, 566.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (279.76, 137.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (478, 1349, 2141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5467) [M::mem_pestat] mean and std.dev: (1281.57, 867.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 246, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364) [M::mem_pestat] mean and std.dev: (346.14, 270.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 106, #reads = 1080643 mem = 536878299 peak = 1091633152 [M::process] read 692718 sequences (100000228 bp) in 2.228 CPU sec, 2.351 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284505, 19, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 273, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455) [M::mem_pestat] mean and std.dev: (346.85, 301.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (281.67, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (464, 951, 3347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9113) [M::mem_pestat] mean and std.dev: (2059.74, 2348.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 292, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (313.91, 156.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693344 reads in 141.190 CPU sec, 4.512 real sec [M::mem_process_seqs] Processed 692596 reads in 89.847 CPU sec, 2.831 real sec [M::process] read 692398 sequences (100000202 bp) in 1.779 CPU sec, 1.841 real sec... sorting block 107, #reads = 1080901 mem = 536877173 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284659, 21, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 262, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (257.98, 101.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 260, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (282.40, 138.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (249, 822, 5075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14727) [M::mem_pestat] mean and std.dev: (2004.62, 2098.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 328, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3063) [M::mem_pestat] mean and std.dev: (691.68, 759.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691970 sequences (100000085 bp) in 2.396 CPU sec, 2.549 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284487, 20, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 588, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2976) [M::mem_pestat] mean and std.dev: (803.24, 729.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 260, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (282.99, 138.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (506, 701, 5882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16634) [M::mem_pestat] mean and std.dev: (2573.30, 3122.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 327, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (289.03, 149.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692718 reads in 139.161 CPU sec, 4.433 real sec [M::mem_process_seqs] Processed 692398 reads in 89.827 CPU sec, 2.832 real sec [M::process] read 692014 sequences (100000221 bp) in 1.646 CPU sec, 1.693 real sec... sorting block 108, #reads = 1081341 mem = 536878736 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 283717, 19, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 341, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502) [M::mem_pestat] mean and std.dev: (406.77, 338.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 261, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (283.72, 138.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (587, 840, 2375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5951) [M::mem_pestat] mean and std.dev: (1344.11, 1256.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7739) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (282.98, 157.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691770 sequences (100000047 bp) in 2.453 CPU sec, 2.595 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284170, 20, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 306, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2512) [M::mem_pestat] mean and std.dev: (432.53, 392.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 263, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (284.74, 138.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 840, 1808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4900) [M::mem_pestat] mean and std.dev: (1008.65, 959.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 305, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1594) [M::mem_pestat] mean and std.dev: (440.55, 323.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691970 reads in 137.080 CPU sec, 4.365 real sec [M::process] read 691840 sequences (100000232 bp) in 1.570 CPU sec, 1.608 real sec... [M::mem_process_seqs] Processed 692014 reads in 90.993 CPU sec, 2.876 real sec sorting block 109, #reads = 1082199 mem = 536882631 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 283821, 22, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 292, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2310) [M::mem_pestat] mean and std.dev: (525.61, 491.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 263, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (285.19, 139.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (296, 1272, 5005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14423) [M::mem_pestat] mean and std.dev: (2450.95, 2860.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 324, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338) [M::mem_pestat] mean and std.dev: (415.64, 316.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 110, #reads = 1081698 mem = 536881814 peak = 1091633152 [M::process] read 691362 sequences (100000016 bp) in 2.178 CPU sec, 2.300 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 284132, 22, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 401, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828) [M::mem_pestat] mean and std.dev: (517.84, 450.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 263, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (285.37, 139.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (465, 2278, 2418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6324) [M::mem_pestat] mean and std.dev: (1544.14, 1220.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 350, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419) [M::mem_pestat] mean and std.dev: (393.41, 278.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691770 reads in 137.178 CPU sec, 4.378 real sec [M::process] read 696074 sequences (100000046 bp) in 1.575 CPU sec, 1.595 real sec... [M::mem_process_seqs] Processed 691840 reads in 90.272 CPU sec, 2.859 real sec sorting block 111, #reads = 1078703 mem = 536880619 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 283470, 13, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 323, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (351.26, 255.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 265, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (286.31, 139.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 391, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2848) [M::mem_pestat] mean and std.dev: (491.55, 368.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 279, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (325.71, 219.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702350 sequences (100000245 bp) in 2.248 CPU sec, 2.359 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285295, 15, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (186.42, 73.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (274.15, 137.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 434, 2671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7641) [M::mem_pestat] mean and std.dev: (980.50, 1129.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 451, 1103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925) [M::mem_pestat] mean and std.dev: (596.05, 617.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3836) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691362 reads in 138.765 CPU sec, 4.415 real sec [M::process] read 701236 sequences (100000179 bp) in 1.508 CPU sec, 1.514 real sec... [M::mem_process_seqs] Processed 696074 reads in 91.038 CPU sec, 2.874 real sec sorting block 112, #reads = 1079931 mem = 536877951 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 287723, 27, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 348, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (373.34, 248.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 230, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (258.46, 131.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 690, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3396) [M::mem_pestat] mean and std.dev: (742.28, 668.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 443, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1896) [M::mem_pestat] mean and std.dev: (520.83, 385.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700666 sequences (100000147 bp) in 2.184 CPU sec, 2.346 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 287671, 18, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 210, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (239.18, 166.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (262.58, 134.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 626, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027) [M::mem_pestat] mean and std.dev: (516.53, 266.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 285, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1532) [M::mem_pestat] mean and std.dev: (416.00, 315.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699976 sequences (100000112 bp) in 1.500 CPU sec, 1.499 real sec... [M::mem_process_seqs] Processed 702350 reads in 144.097 CPU sec, 4.580 real sec [M::mem_process_seqs] Processed 701236 reads in 90.607 CPU sec, 2.866 real sec sorting block 113, #reads = 1081907 mem = 536884567 peak = 1091633152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 287371, 21, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 275, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (279.71, 126.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (263.57, 134.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (576, 738, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120) [M::mem_pestat] mean and std.dev: (823.35, 483.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3968) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 229, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1404) [M::mem_pestat] mean and std.dev: (361.55, 316.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699888 sequences (100000196 bp) in 2.260 CPU sec, 2.398 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 287061, 16, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 367, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234) [M::mem_pestat] mean and std.dev: (297.83, 171.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (266.26, 136.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (84, 798, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5283) [M::mem_pestat] mean and std.dev: (778.33, 691.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7016) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 296, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166) [M::mem_pestat] mean and std.dev: (313.98, 203.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699956 sequences (100000044 bp) in 1.530 CPU sec, 1.525 real sec... [M::mem_process_seqs] Processed 700666 reads in 142.415 CPU sec, 4.526 real sec sorting block 114, #reads = 1085202 mem = 536881660 peak = 1091633152 [M::mem_process_seqs] Processed 699976 reads in 91.261 CPU sec, 2.886 real sec sorting block 115, #reads = 1084635 mem = 536880019 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286843, 11, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 283, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (292.43, 219.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (265.47, 135.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 1067, 3277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9373) [M::mem_pestat] mean and std.dev: (1428.36, 1339.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 316, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (335.38, 166.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1524) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699118 sequences (100000167 bp) in 2.313 CPU sec, 2.442 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286709, 10, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 352, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1592) [M::mem_pestat] mean and std.dev: (407.73, 335.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (265.60, 135.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (523, 1513, 1849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4501) [M::mem_pestat] mean and std.dev: (1195.33, 918.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 292, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (305.67, 160.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698722 sequences (100000139 bp) in 1.510 CPU sec, 1.511 real sec... [M::mem_process_seqs] Processed 699888 reads in 145.228 CPU sec, 4.620 real sec [M::mem_process_seqs] Processed 699956 reads in 93.175 CPU sec, 2.944 real sec sorting block 116, #reads = 1083025 mem = 536879191 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 287443, 9, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 363, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480) [M::mem_pestat] mean and std.dev: (402.82, 311.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (267.74, 135.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 241, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (271.18, 142.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697896 sequences (100000271 bp) in 2.365 CPU sec, 2.473 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 286487, 26, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 231, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (248.73, 135.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (269.94, 137.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (249, 784, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2766) [M::mem_pestat] mean and std.dev: (626.43, 441.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3605) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 274, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (331.57, 251.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697118 sequences (100000206 bp) in 1.490 CPU sec, 1.489 real sec... [M::mem_process_seqs] Processed 699118 reads in 144.458 CPU sec, 4.589 real sec [M::mem_process_seqs] Processed 698722 reads in 92.229 CPU sec, 2.917 real sec sorting block 117, #reads = 1082147 mem = 536879530 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 286414, 19, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 195, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (239.98, 112.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (271.95, 137.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 810, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3173) [M::mem_pestat] mean and std.dev: (766.39, 576.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4141) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 404, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (386.04, 186.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697328 sequences (100000025 bp) in 2.651 CPU sec, 2.765 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286363, 16, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 258, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (285.13, 176.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (275.60, 139.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 813, 1028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2372) [M::mem_pestat] mean and std.dev: (709.07, 613.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 286, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (282.12, 126.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697896 reads in 141.412 CPU sec, 4.487 real sec [M::process] read 697602 sequences (100000088 bp) in 1.499 CPU sec, 1.500 real sec... sorting block 118, #reads = 1081727 mem = 536878452 peak = 1122557952 [M::mem_process_seqs] Processed 697118 reads in 89.712 CPU sec, 2.836 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 286228, 13, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 302, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1439) [M::mem_pestat] mean and std.dev: (330.83, 239.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (274.44, 138.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 493, 1883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5293) [M::mem_pestat] mean and std.dev: (1090.00, 1460.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6998) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 291, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2063) [M::mem_pestat] mean and std.dev: (338.97, 265.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 119, #reads = 1082539 mem = 536879371 peak = 1122557952 [M::process] read 697264 sequences (100000151 bp) in 2.396 CPU sec, 2.546 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 286312, 21, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 305, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (282.02, 129.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (273.59, 138.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 702, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5119) [M::mem_pestat] mean and std.dev: (848.53, 789.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 681, 2131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5867) [M::mem_pestat] mean and std.dev: (1540.57, 1639.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697328 reads in 141.017 CPU sec, 4.480 real sec [M::process] read 698292 sequences (100000189 bp) in 1.519 CPU sec, 1.515 real sec... [M::mem_process_seqs] Processed 697602 reads in 90.564 CPU sec, 2.858 real sec sorting block 120, #reads = 1081642 mem = 536877785 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285912, 30, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 245, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (242.28, 107.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (273.95, 137.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (564, 1480, 2202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5478) [M::mem_pestat] mean and std.dev: (1496.76, 1108.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 266, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2586) [M::mem_pestat] mean and std.dev: (457.15, 489.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3379) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699164 sequences (100000288 bp) in 2.376 CPU sec, 2.507 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 286585, 25, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 282, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2939) [M::mem_pestat] mean and std.dev: (447.00, 461.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (269.78, 135.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (407, 1106, 2002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5192) [M::mem_pestat] mean and std.dev: (1227.48, 971.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 300, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1743) [M::mem_pestat] mean and std.dev: (447.55, 407.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700056 sequences (100000147 bp) in 1.510 CPU sec, 1.506 real sec... [M::mem_process_seqs] Processed 697264 reads in 147.019 CPU sec, 4.697 real sec [M::mem_process_seqs] Processed 698292 reads in 91.801 CPU sec, 2.901 real sec sorting block 121, #reads = 1082726 mem = 536878473 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 286595, 37, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 284, 2136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6136) [M::mem_pestat] mean and std.dev: (617.06, 960.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (266.53, 134.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 2710, 4664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13076) [M::mem_pestat] mean and std.dev: (2908.22, 2683.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 257, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1470) [M::mem_pestat] mean and std.dev: (323.53, 277.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699754 sequences (100000048 bp) in 2.328 CPU sec, 2.461 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 287285, 22, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 253, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (306.80, 212.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (263.85, 133.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (469, 672, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3430) [M::mem_pestat] mean and std.dev: (1061.90, 987.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 232, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1802) [M::mem_pestat] mean and std.dev: (341.26, 329.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699532 sequences (100000018 bp) in 1.560 CPU sec, 1.565 real sec... [M::mem_process_seqs] Processed 699164 reads in 146.708 CPU sec, 4.662 real sec [M::mem_process_seqs] Processed 700056 reads in 94.335 CPU sec, 2.977 real sec sorting block 122, #reads = 1082508 mem = 536878041 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 286959, 24, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (330.63, 230.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (265.04, 134.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 849, 5072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14546) [M::mem_pestat] mean and std.dev: (2260.79, 2581.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19283) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 399, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (342.91, 137.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 123, #reads = 1082181 mem = 536877356 peak = 1122557952 [M::process] read 699420 sequences (100000046 bp) in 2.371 CPU sec, 2.473 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 286836, 15, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 282, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (283.24, 165.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (265.31, 134.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (704, 1014, 2157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5063) [M::mem_pestat] mean and std.dev: (1240.40, 863.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 237, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (231.19, 95.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699796 sequences (100000226 bp) in 1.540 CPU sec, 1.541 real sec... [M::mem_process_seqs] Processed 699754 reads in 149.879 CPU sec, 4.764 real sec [M::mem_process_seqs] Processed 699532 reads in 96.280 CPU sec, 3.041 real sec sorting block 124, #reads = 1082013 mem = 536877912 peak = 1122557952 [M::process] read 698984 sequences (100000184 bp) in 2.365 CPU sec, 2.489 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 286764, 10, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 204, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (285.39, 193.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (265.32, 134.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (537, 1399, 1869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4533) [M::mem_pestat] mean and std.dev: (888.62, 627.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 368, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (384.61, 216.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698000 sequences (100000251 bp) in 1.530 CPU sec, 1.527 real sec... [M::mem_process_seqs] Processed 699796 reads in 85.142 CPU sec, 2.685 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 286950, 19, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 337, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2408) [M::mem_pestat] mean and std.dev: (554.78, 405.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (265.54, 134.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (459, 2294, 4570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12792) [M::mem_pestat] mean and std.dev: (2611.53, 2308.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16903) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 275, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (286.69, 107.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 286691, 20, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 295, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (274.64, 108.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (267.39, 135.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 411, 1112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2844) [M::mem_pestat] mean and std.dev: (602.42, 496.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 278, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (299.17, 182.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1555) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697188 sequences (100000217 bp) in 1.921 CPU sec, 1.929 real sec... [M::mem_process_seqs] Processed 699420 reads in 235.011 CPU sec, 7.455 real sec sorting block 125, #reads = 1080668 mem = 536881179 peak = 1122557952 [M::mem_process_seqs] Processed 698984 reads in 111.309 CPU sec, 3.545 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285876, 28, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 608, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2249) [M::mem_pestat] mean and std.dev: (479.67, 392.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (269.83, 136.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (471, 699, 2977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7989) [M::mem_pestat] mean and std.dev: (1280.35, 1548.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 249, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (344.94, 265.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 126, #reads = 1080750 mem = 536880193 peak = 1122557952 [M::process] read 696950 sequences (100000144 bp) in 2.541 CPU sec, 2.688 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 286134, 19, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 323, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (313.36, 188.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.27, 136.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (423, 1349, 2845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7689) [M::mem_pestat] mean and std.dev: (1600.00, 1072.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 312, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (306.77, 138.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698000 reads in 147.992 CPU sec, 4.707 real sec [M::process] read 696820 sequences (100000169 bp) in 1.571 CPU sec, 1.579 real sec... [M::mem_process_seqs] Processed 697188 reads in 94.123 CPU sec, 2.973 real sec sorting block 127, #reads = 1080975 mem = 536879228 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 286386, 34, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 225, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (277.63, 160.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.62, 135.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (176, 388, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2795) [M::mem_pestat] mean and std.dev: (417.76, 392.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 263, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (293.74, 187.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702588 sequences (100000288 bp) in 2.629 CPU sec, 2.763 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285341, 20, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 502, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3226) [M::mem_pestat] mean and std.dev: (735.44, 572.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (273.03, 136.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 416, 2408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7088) [M::mem_pestat] mean and std.dev: (943.50, 1052.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 367, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412) [M::mem_pestat] mean and std.dev: (397.07, 288.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696950 reads in 146.622 CPU sec, 4.647 real sec [M::process] read 698514 sequences (100000265 bp) in 1.590 CPU sec, 1.589 real sec... [M::mem_process_seqs] Processed 696820 reads in 94.920 CPU sec, 2.991 real sec sorting block 128, #reads = 1081936 mem = 536880978 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 287328, 19, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 237, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (235.97, 84.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 231, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (256.41, 127.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (137, 425, 1600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4526) [M::mem_pestat] mean and std.dev: (946.21, 1150.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5989) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 313, 1613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4413) [M::mem_pestat] mean and std.dev: (886.57, 1242.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697284 sequences (100000230 bp) in 2.441 CPU sec, 2.565 real sec... [M::process] read 697270 sequences (100000067 bp) in 1.550 CPU sec, 1.557 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 286221, 15, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 229, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (406.77, 380.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (266.65, 132.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 442, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7675) [M::mem_pestat] mean and std.dev: (1562.07, 2375.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11066) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 351, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (375.65, 211.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702588 reads in 171.943 CPU sec, 5.484 real sec sorting block 129, #reads = 1079622 mem = 536888041 peak = 1122557952 [M::mem_process_seqs] Processed 698514 reads in 111.386 CPU sec, 3.560 real sec sorting block 130, #reads = 1071078 mem = 536882277 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285595, 21, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 275, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (238.98, 111.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (269.01, 133.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (421, 962, 2027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5239) [M::mem_pestat] mean and std.dev: (1238.70, 1171.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 364, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2416) [M::mem_pestat] mean and std.dev: (525.63, 475.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696300 sequences (100000159 bp) in 2.328 CPU sec, 2.459 real sec... [M::process] read 696118 sequences (100000164 bp) in 1.540 CPU sec, 1.541 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 285824, 14, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 450, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3116) [M::mem_pestat] mean and std.dev: (612.42, 690.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (268.27, 132.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (422, 637, 4225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11831) [M::mem_pestat] mean and std.dev: (1783.00, 1953.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 325, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235) [M::mem_pestat] mean and std.dev: (325.11, 203.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1576) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697284 reads in 162.326 CPU sec, 5.163 real sec [M::mem_process_seqs] Processed 697270 reads in 104.163 CPU sec, 3.303 real sec sorting block 131, #reads = 1074476 mem = 536878905 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285555, 21, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 373, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (543.35, 444.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.39, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (257, 904, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587) [M::mem_pestat] mean and std.dev: (989.95, 938.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4745) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 335, 726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1686) [M::mem_pestat] mean and std.dev: (406.78, 268.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2166) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695994 sequences (100000146 bp) in 2.308 CPU sec, 2.438 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285186, 15, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 446, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2244) [M::mem_pestat] mean and std.dev: (518.67, 441.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.66, 135.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (366, 511, 905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983) [M::mem_pestat] mean and std.dev: (510.64, 355.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 243, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1707) [M::mem_pestat] mean and std.dev: (353.09, 339.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695636 sequences (100000125 bp) in 1.520 CPU sec, 1.521 real sec... [M::mem_process_seqs] Processed 696300 reads in 153.997 CPU sec, 4.910 real sec [M::mem_process_seqs] Processed 696118 reads in 98.694 CPU sec, 3.199 real sec sorting block 132, #reads = 1076601 mem = 536880563 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285314, 19, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 224, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (250.00, 143.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (272.98, 135.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (141, 1553, 5133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15117) [M::mem_pestat] mean and std.dev: (2455.32, 2800.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20109) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 293, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1470) [M::mem_pestat] mean and std.dev: (394.26, 294.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695356 sequences (100000044 bp) in 2.326 CPU sec, 2.444 real sec... [M::process] read 695934 sequences (100000030 bp) in 1.530 CPU sec, 1.531 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285348, 22, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 210, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (234.07, 118.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.34, 137.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (403, 1881, 3334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9196) [M::mem_pestat] mean and std.dev: (2003.84, 2070.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12127) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (311, 463, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3842) [M::mem_pestat] mean and std.dev: (763.21, 740.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695994 reads in 160.554 CPU sec, 5.171 real sec [M::mem_process_seqs] Processed 695636 reads in 99.294 CPU sec, 3.147 real sec sorting block 133, #reads = 1077351 mem = 536880153 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284717, 18, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 375, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2595) [M::mem_pestat] mean and std.dev: (556.21, 556.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (276.09, 137.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 456, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2248) [M::mem_pestat] mean and std.dev: (508.06, 358.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 356, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1398) [M::mem_pestat] mean and std.dev: (475.70, 371.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 134, #reads = 1076117 mem = 536883641 peak = 1122557952 [M::process] read 696348 sequences (100000282 bp) in 2.320 CPU sec, 2.439 real sec... [M::process] read 695410 sequences (100000119 bp) in 1.520 CPU sec, 1.519 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 285283, 26, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 280, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (269.40, 143.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.66, 136.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 1059, 1683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4545) [M::mem_pestat] mean and std.dev: (884.41, 699.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 307, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (300.57, 134.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695356 reads in 161.279 CPU sec, 5.200 real sec [M::mem_process_seqs] Processed 695934 reads in 102.125 CPU sec, 3.248 real sec sorting block 135, #reads = 1075580 mem = 536879485 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 285337, 16, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 318, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (287.69, 154.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (272.14, 135.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (331, 1072, 2818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7792) [M::mem_pestat] mean and std.dev: (1428.56, 1549.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 296, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (337.46, 181.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697162 sequences (100000256 bp) in 2.387 CPU sec, 2.493 real sec... [M::process] read 707902 sequences (100000106 bp) in 1.580 CPU sec, 1.580 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285085, 30, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 235, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (307.45, 249.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (274.52, 137.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 576, 1644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4866) [M::mem_pestat] mean and std.dev: (954.40, 966.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 278, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1901) [M::mem_pestat] mean and std.dev: (469.50, 463.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696348 reads in 158.062 CPU sec, 5.022 real sec [M::mem_process_seqs] Processed 695410 reads in 100.539 CPU sec, 3.186 real sec sorting block 136, #reads = 1074857 mem = 536878671 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285588, 19, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 320, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (307.72, 179.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (271.06, 136.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (401, 1653, 4031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11291) [M::mem_pestat] mean and std.dev: (2387.58, 2589.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 561, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (603.59, 407.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3300) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705558 sequences (100000080 bp) in 2.449 CPU sec, 2.577 real sec... [M::process] read 702826 sequences (100000244 bp) in 1.570 CPU sec, 1.563 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 289472, 27, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 339, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1564) [M::mem_pestat] mean and std.dev: (366.10, 234.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 216, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (242.79, 122.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 386, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2591) [M::mem_pestat] mean and std.dev: (545.58, 625.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 297, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (260.87, 93.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697162 reads in 169.052 CPU sec, 5.454 real sec [M::mem_process_seqs] Processed 707902 reads in 108.783 CPU sec, 3.452 real sec sorting block 137, #reads = 1074480 mem = 536881631 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 289002, 18, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 269, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (275.83, 180.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 138, #reads = 1074520 mem = 536882473 peak = 1122557952 [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (248.42, 125.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (331, 1157, 3097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8629) [M::mem_pestat] mean and std.dev: (2289.33, 2667.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (304.55, 179.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701562 sequences (100000275 bp) in 2.341 CPU sec, 2.452 real sec... [M::process] read 700970 sequences (100000058 bp) in 1.560 CPU sec, 1.561 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 287654, 15, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 281, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1601) [M::mem_pestat] mean and std.dev: (416.21, 370.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 228, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (255.15, 128.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 397, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3186) [M::mem_pestat] mean and std.dev: (470.38, 486.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4194) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 299, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2930) [M::mem_pestat] mean and std.dev: (577.41, 589.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705558 reads in 164.681 CPU sec, 5.237 real sec [M::mem_process_seqs] Processed 702826 reads in 103.171 CPU sec, 3.274 real sec sorting block 139, #reads = 1075158 mem = 536880852 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 287495, 25, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 367, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3333) [M::mem_pestat] mean and std.dev: (745.72, 800.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (259.29, 130.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (402, 829, 1964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5088) [M::mem_pestat] mean and std.dev: (1147.84, 946.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6650) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 502, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029) [M::mem_pestat] mean and std.dev: (577.44, 474.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701498 sequences (100000094 bp) in 2.345 CPU sec, 2.450 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 287549, 13, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (196.24, 63.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (260.89, 131.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1112, 1602, 2730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5966) [M::mem_pestat] mean and std.dev: (1788.46, 1255.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 354, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831) [M::mem_pestat] mean and std.dev: (412.63, 366.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2373) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700792 sequences (100000301 bp) in 1.560 CPU sec, 1.559 real sec... [M::mem_process_seqs] Processed 701562 reads in 159.998 CPU sec, 5.129 real sec [M::mem_process_seqs] Processed 700970 reads in 99.820 CPU sec, 3.159 real sec sorting block 140, #reads = 1079987 mem = 536885805 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 287638, 15, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 212, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (259.97, 168.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (260.03, 131.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 315, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2795) [M::mem_pestat] mean and std.dev: (580.27, 459.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 207, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (241.73, 136.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699126 sequences (100000066 bp) in 2.350 CPU sec, 2.458 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 287473, 22, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (396.73, 401.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 234, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (262.03, 132.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (540, 1119, 2041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5043) [M::mem_pestat] mean and std.dev: (1217.60, 865.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 350, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (363.12, 265.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698770 sequences (100000044 bp) in 1.560 CPU sec, 1.556 real sec... [M::mem_process_seqs] Processed 701498 reads in 157.295 CPU sec, 5.004 real sec [M::mem_process_seqs] Processed 700792 reads in 99.965 CPU sec, 3.166 real sec sorting block 141, #reads = 1078599 mem = 536881695 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 287251, 28, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 237, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2289) [M::mem_pestat] mean and std.dev: (374.78, 398.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (266.01, 132.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (91, 413, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4135) [M::mem_pestat] mean and std.dev: (793.86, 894.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 297, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (272.90, 162.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698452 sequences (100000089 bp) in 2.449 CPU sec, 2.558 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 286402, 17, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 265, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (268.80, 129.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (267.55, 134.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 1940, 2714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8068) [M::mem_pestat] mean and std.dev: (1570.24, 1581.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10745) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171) [M::mem_pestat] mean and std.dev: (310.68, 236.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697628 sequences (100000164 bp) in 1.540 CPU sec, 1.537 real sec... [M::mem_process_seqs] Processed 699126 reads in 154.525 CPU sec, 4.913 real sec sorting block 142, #reads = 1079205 mem = 536879015 peak = 1122557952 [M::mem_process_seqs] Processed 698770 reads in 99.150 CPU sec, 3.134 real sec sorting block 143, #reads = 1077438 mem = 536878782 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 286582, 27, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 283, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1749) [M::mem_pestat] mean and std.dev: (299.06, 174.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (268.68, 135.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (732, 918, 1957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4407) [M::mem_pestat] mean and std.dev: (1308.96, 913.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5632) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 375, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1649) [M::mem_pestat] mean and std.dev: (451.06, 311.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697518 sequences (100000046 bp) in 2.754 CPU sec, 2.891 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 287082, 20, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 257, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258) [M::mem_pestat] mean and std.dev: (288.51, 176.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.19, 135.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (502, 893, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3514) [M::mem_pestat] mean and std.dev: (929.39, 865.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 291, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (344.76, 209.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699176 sequences (100000272 bp) in 1.550 CPU sec, 1.546 real sec... [M::mem_process_seqs] Processed 698452 reads in 150.303 CPU sec, 4.771 real sec [M::mem_process_seqs] Processed 697628 reads in 95.887 CPU sec, 3.032 real sec sorting block 144, #reads = 1078010 mem = 536881816 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 286439, 19, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 263, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531) [M::mem_pestat] mean and std.dev: (316.33, 199.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (271.87, 136.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 639, 2730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7952) [M::mem_pestat] mean and std.dev: (1332.68, 1549.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 282, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (286.84, 140.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699082 sequences (100000105 bp) in 2.426 CPU sec, 2.528 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 287312, 12, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 276, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2084) [M::mem_pestat] mean and std.dev: (478.28, 397.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (266.23, 133.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 1549, 2838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7760) [M::mem_pestat] mean and std.dev: (1658.67, 1407.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 287, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (297.32, 145.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697518 reads in 145.377 CPU sec, 4.621 real sec [M::process] read 698866 sequences (100000167 bp) in 1.550 CPU sec, 1.546 real sec... [M::mem_process_seqs] Processed 699176 reads in 93.082 CPU sec, 2.936 real sec sorting block 145, #reads = 1080576 mem = 536877292 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 286600, 8, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 348, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346) [M::mem_pestat] mean and std.dev: (339.31, 227.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (266.00, 133.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 347, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763) [M::mem_pestat] mean and std.dev: (391.48, 268.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 698350 sequences (100000274 bp) in 2.365 CPU sec, 2.477 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 287143, 15, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 406, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1925) [M::mem_pestat] mean and std.dev: (408.20, 293.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (266.63, 133.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 883, 3657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9701) [M::mem_pestat] mean and std.dev: (2161.80, 2348.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 246, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1518) [M::mem_pestat] mean and std.dev: (377.89, 309.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698492 sequences (100000273 bp) in 1.540 CPU sec, 1.542 real sec... [M::mem_process_seqs] Processed 699082 reads in 147.080 CPU sec, 4.685 real sec sorting block 146, #reads = 1082402 mem = 536880189 peak = 1122557952 [M::mem_process_seqs] Processed 698866 reads in 95.056 CPU sec, 3.019 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 286534, 26, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 215, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (250.66, 124.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (266.60, 133.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1028, 1090, 2748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6188) [M::mem_pestat] mean and std.dev: (1703.35, 1220.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 276, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1994) [M::mem_pestat] mean and std.dev: (435.78, 367.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 147, #reads = 1082146 mem = 536882665 peak = 1122557952 [M::process] read 698298 sequences (100000120 bp) in 2.448 CPU sec, 2.567 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 286965, 15, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 352, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1557) [M::mem_pestat] mean and std.dev: (391.85, 270.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (267.03, 133.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1026, 1244, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (468, 1863) [M::mem_pestat] mean and std.dev: (1201.75, 107.81) [M::mem_pestat] low and high boundaries for proper pairs: (189, 2142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 370, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272) [M::mem_pestat] mean and std.dev: (398.82, 275.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698350 reads in 145.771 CPU sec, 4.631 real sec [M::process] read 698026 sequences (100000040 bp) in 1.540 CPU sec, 1.545 real sec... [M::mem_process_seqs] Processed 698492 reads in 92.708 CPU sec, 2.927 real sec sorting block 148, #reads = 1081589 mem = 536880331 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 286586, 12, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 264, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2383) [M::mem_pestat] mean and std.dev: (349.03, 292.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.31, 134.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 665, 2538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7090) [M::mem_pestat] mean and std.dev: (1002.67, 968.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 360, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1583) [M::mem_pestat] mean and std.dev: (412.33, 281.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698758 sequences (100000217 bp) in 2.346 CPU sec, 2.444 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 286335, 19, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 302, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (393.11, 293.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.84, 134.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (230, 288, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302) [M::mem_pestat] mean and std.dev: (696.00, 644.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 343, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1799) [M::mem_pestat] mean and std.dev: (442.32, 380.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698668 sequences (100000169 bp) in 1.550 CPU sec, 1.552 real sec... [M::mem_process_seqs] Processed 698298 reads in 146.427 CPU sec, 4.657 real sec [M::mem_process_seqs] Processed 698026 reads in 94.229 CPU sec, 3.228 real sec sorting block 149, #reads = 1083837 mem = 536881590 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 286351, 21, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 274, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1202) [M::mem_pestat] mean and std.dev: (336.67, 225.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (267.05, 134.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (525, 1425, 8618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24804) [M::mem_pestat] mean and std.dev: (3130.71, 3526.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32897) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 367, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (324.64, 123.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697762 sequences (100000147 bp) in 2.522 CPU sec, 2.612 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286405, 23, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 207, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (230.69, 125.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (267.24, 134.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 429, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2438) [M::mem_pestat] mean and std.dev: (581.59, 599.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 301, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373) [M::mem_pestat] mean and std.dev: (291.36, 180.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697294 sequences (100000295 bp) in 1.580 CPU sec, 1.579 real sec... [M::mem_process_seqs] Processed 698758 reads in 156.411 CPU sec, 5.211 real sec [M::mem_process_seqs] Processed 698668 reads in 98.314 CPU sec, 3.110 real sec sorting block 150, #reads = 1080856 mem = 536887045 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285857, 41, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 260, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (272.03, 145.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.79, 134.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (116, 783, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4136) [M::mem_pestat] mean and std.dev: (738.05, 785.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 312, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (292.57, 161.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 151, #reads = 1078877 mem = 536883658 peak = 1122557952 [M::process] read 702332 sequences (100000172 bp) in 2.551 CPU sec, 2.633 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 285749, 20, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 269, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634) [M::mem_pestat] mean and std.dev: (385.65, 359.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (271.33, 136.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 585, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3558) [M::mem_pestat] mean and std.dev: (507.61, 540.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 407, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688) [M::mem_pestat] mean and std.dev: (430.91, 278.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2166) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698214 sequences (100000137 bp) in 1.580 CPU sec, 1.578 real sec... [M::mem_process_seqs] Processed 697762 reads in 152.779 CPU sec, 4.851 real sec [M::mem_process_seqs] Processed 697294 reads in 98.033 CPU sec, 3.103 real sec sorting block 152, #reads = 1077235 mem = 536877926 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 287155, 24, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 238, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (275.02, 158.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (256.74, 128.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (906, 1372, 3394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8370) [M::mem_pestat] mean and std.dev: (2101.08, 2018.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10858) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 310, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2141) [M::mem_pestat] mean and std.dev: (464.95, 387.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2794) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695574 sequences (100000028 bp) in 2.509 CPU sec, 2.592 real sec... [M::process] read 695522 sequences (100000068 bp) in 1.540 CPU sec, 1.537 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285717, 16, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 276, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (256.26, 112.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (266.91, 133.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 1007, 1860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5016) [M::mem_pestat] mean and std.dev: (1081.31, 811.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 322, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779) [M::mem_pestat] mean and std.dev: (488.29, 433.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702332 reads in 169.524 CPU sec, 5.395 real sec [M::mem_process_seqs] Processed 698214 reads in 104.964 CPU sec, 3.329 real sec sorting block 153, #reads = 1071973 mem = 536878621 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285346, 18, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 291, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (357.37, 270.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.78, 139.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (427, 986, 1386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3304) [M::mem_pestat] mean and std.dev: (825.00, 530.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 493, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2642) [M::mem_pestat] mean and std.dev: (612.43, 435.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695646 sequences (100000109 bp) in 2.480 CPU sec, 2.560 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 285221, 14, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 331, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (293.35, 122.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.70, 139.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 1392, 3653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10411) [M::mem_pestat] mean and std.dev: (1573.93, 1497.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 297, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (333.66, 256.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695482 sequences (100000056 bp) in 1.530 CPU sec, 1.527 real sec... [M::mem_process_seqs] Processed 695574 reads in 144.997 CPU sec, 4.604 real sec [M::mem_process_seqs] Processed 695522 reads in 93.534 CPU sec, 2.953 real sec sorting block 154, #reads = 1076806 mem = 536878340 peak = 1122557952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285247, 22, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 264, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3210) [M::mem_pestat] mean and std.dev: (615.73, 729.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (276.12, 138.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (196, 831, 2476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7036) [M::mem_pestat] mean and std.dev: (1681.14, 1924.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 299, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (275.51, 146.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695242 sequences (100000048 bp) in 2.482 CPU sec, 2.559 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 285143, 17, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 354, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651) [M::mem_pestat] mean and std.dev: (384.59, 323.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (276.86, 139.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (433, 732, 2262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5920) [M::mem_pestat] mean and std.dev: (1157.59, 957.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 321, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828) [M::mem_pestat] mean and std.dev: (328.21, 212.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2381) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694668 sequences (100000197 bp) in 1.530 CPU sec, 1.532 real sec... [M::mem_process_seqs] Processed 695646 reads in 147.401 CPU sec, 4.686 real sec sorting block 155, #reads = 1079599 mem = 536878471 peak = 1122557952 [M::mem_process_seqs] Processed 695482 reads in 94.862 CPU sec, 2.996 real sec sorting block 156, #reads = 1079324 mem = 536883496 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285443, 22, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 268, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (235.46, 108.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.75, 140.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 832, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3140) [M::mem_pestat] mean and std.dev: (822.24, 636.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 310, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (338.66, 219.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694714 sequences (100000191 bp) in 2.550 CPU sec, 2.665 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284757, 16, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 243, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (280.42, 173.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (279.65, 141.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (696, 926, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3156) [M::mem_pestat] mean and std.dev: (886.57, 488.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 294, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268) [M::mem_pestat] mean and std.dev: (304.00, 170.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1611) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694506 sequences (100000066 bp) in 1.550 CPU sec, 1.550 real sec... [M::mem_process_seqs] Processed 695242 reads in 145.935 CPU sec, 4.636 real sec [M::mem_process_seqs] Processed 694668 reads in 93.428 CPU sec, 2.956 real sec sorting block 157, #reads = 1080033 mem = 536880879 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284664, 15, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 311, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1581) [M::mem_pestat] mean and std.dev: (389.51, 247.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (281.02, 142.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 686, 2495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6709) [M::mem_pestat] mean and std.dev: (1124.46, 1658.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 285, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (280.07, 139.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694830 sequences (100000168 bp) in 2.418 CPU sec, 2.543 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 284982, 17, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 274, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (353.82, 268.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (282.00, 143.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 286, 8515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25187) [M::mem_pestat] mean and std.dev: (2343.94, 3452.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 33523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 268, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (237.61, 95.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694714 reads in 145.662 CPU sec, 4.648 real sec [M::process] read 694352 sequences (100000189 bp) in 1.575 CPU sec, 1.582 real sec... [M::mem_process_seqs] Processed 694506 reads in 95.314 CPU sec, 3.033 real sec sorting block 158, #reads = 1080770 mem = 536877163 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284855, 17, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 241, 1982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5644) [M::mem_pestat] mean and std.dev: (1007.85, 1442.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (281.18, 143.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (486, 1000, 3749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10275) [M::mem_pestat] mean and std.dev: (1873.94, 1699.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 336, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (330.38, 175.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694318 sequences (100000079 bp) in 2.374 CPU sec, 2.461 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284530, 19, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 250, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (242.06, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (280.58, 141.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (705, 918, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2538) [M::mem_pestat] mean and std.dev: (731.33, 387.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 245, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (255.72, 133.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694830 reads in 146.633 CPU sec, 4.661 real sec [M::process] read 695584 sequences (100000031 bp) in 1.570 CPU sec, 1.574 real sec... sorting block 159, #reads = 1079214 mem = 536878772 peak = 1122684928 [M::mem_process_seqs] Processed 694352 reads in 94.882 CPU sec, 2.994 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284630, 8, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 314, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2053) [M::mem_pestat] mean and std.dev: (384.64, 413.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 253, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (281.05, 142.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 295, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (361.73, 288.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 160, #reads = 1078995 mem = 536879646 peak = 1122684928 [M::process] read 703044 sequences (100000276 bp) in 2.485 CPU sec, 2.561 real sec... [M::process] read 700126 sequences (100000077 bp) in 1.540 CPU sec, 1.535 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284247, 17, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 342, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1505) [M::mem_pestat] mean and std.dev: (369.43, 226.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.68, 138.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 490, 2679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7419) [M::mem_pestat] mean and std.dev: (1359.88, 1680.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 322, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1818) [M::mem_pestat] mean and std.dev: (461.33, 401.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694318 reads in 163.204 CPU sec, 5.194 real sec [M::mem_process_seqs] Processed 695584 reads in 112.428 CPU sec, 3.567 real sec sorting block 161, #reads = 1074734 mem = 536878266 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 288232, 31, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 334, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (258.41, 134.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (256.88, 132.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 1365, 2489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7033) [M::mem_pestat] mean and std.dev: (1712.68, 1771.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 209, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (238.70, 112.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699464 sequences (100000173 bp) in 2.783 CPU sec, 2.906 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 287507, 14, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 234, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (239.41, 105.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (266.21, 137.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 807, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1995) [M::mem_pestat] mean and std.dev: (612.31, 438.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 293, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1369) [M::mem_pestat] mean and std.dev: (365.18, 268.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698346 sequences (100000196 bp) in 1.500 CPU sec, 1.503 real sec... [M::mem_process_seqs] Processed 703044 reads in 147.151 CPU sec, 4.666 real sec [M::mem_process_seqs] Processed 700126 reads in 91.388 CPU sec, 2.890 real sec sorting block 162, #reads = 1072841 mem = 536877478 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 287174, 16, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 300, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538) [M::mem_pestat] mean and std.dev: (338.43, 235.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (268.24, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 988, 1731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3957) [M::mem_pestat] mean and std.dev: (1129.50, 832.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 263, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418) [M::mem_pestat] mean and std.dev: (366.96, 226.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698404 sequences (100000062 bp) in 2.483 CPU sec, 2.555 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 286654, 23, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 433, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (429.45, 286.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (270.77, 138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (365, 523, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1910) [M::mem_pestat] mean and std.dev: (498.25, 344.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 349, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2226) [M::mem_pestat] mean and std.dev: (493.81, 462.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699464 reads in 137.890 CPU sec, 4.389 real sec [M::mem_process_seqs] Processed 698346 reads in 87.897 CPU sec, 2.774 real sec [M::process] read 698298 sequences (100000139 bp) in 1.727 CPU sec, 1.775 real sec... sorting block 163, #reads = 1084646 mem = 536878623 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 286547, 8, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 309, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3617) [M::mem_pestat] mean and std.dev: (635.07, 621.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.20, 138.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 336, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (327.61, 149.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 164, #reads = 1085238 mem = 536880410 peak = 1122684928 [M::process] read 697966 sequences (100000125 bp) in 2.308 CPU sec, 2.454 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 286436, 13, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 332, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498) [M::mem_pestat] mean and std.dev: (409.69, 313.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.37, 138.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1064, 2089, 3560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8552) [M::mem_pestat] mean and std.dev: (2199.62, 1450.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11048) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 293, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (334.08, 177.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698404 reads in 140.039 CPU sec, 4.467 real sec [M::mem_process_seqs] Processed 698298 reads in 90.347 CPU sec, 2.851 real sec [M::process] read 696810 sequences (100000178 bp) in 1.746 CPU sec, 1.776 real sec... sorting block 165, #reads = 1083189 mem = 536880808 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 285718, 28, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 258, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (448.13, 511.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.45, 138.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (425, 1075, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3545) [M::mem_pestat] mean and std.dev: (1046.11, 826.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 298, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (277.67, 128.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695708 sequences (100000277 bp) in 2.461 CPU sec, 2.599 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 286100, 14, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 354, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (279.05, 135.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (275.76, 139.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 870, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3654) [M::mem_pestat] mean and std.dev: (770.00, 651.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (215.53, 95.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697966 reads in 140.048 CPU sec, 4.487 real sec [M::mem_process_seqs] Processed 696810 reads in 89.054 CPU sec, 2.831 real sec [M::process] read 695640 sequences (100000190 bp) in 1.830 CPU sec, 1.880 real sec... sorting block 166, #reads = 1082379 mem = 536881286 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 286099, 23, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 185, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1453) [M::mem_pestat] mean and std.dev: (293.59, 267.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (277.83, 139.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (407, 587, 1858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4760) [M::mem_pestat] mean and std.dev: (1073.17, 1012.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 216, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (242.40, 135.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695826 sequences (100000271 bp) in 2.540 CPU sec, 2.673 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285457, 28, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 288, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774) [M::mem_pestat] mean and std.dev: (445.64, 364.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (278.92, 140.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (884, 1052, 2338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5246) [M::mem_pestat] mean and std.dev: (1464.54, 975.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 364, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (330.06, 155.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695708 reads in 137.548 CPU sec, 4.382 real sec [M::mem_process_seqs] Processed 695640 reads in 88.826 CPU sec, 2.804 real sec [M::process] read 695556 sequences (100000285 bp) in 1.860 CPU sec, 1.915 real sec... sorting block 167, #reads = 1084001 mem = 536877946 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 286016, 24, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 259, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1819) [M::mem_pestat] mean and std.dev: (424.12, 388.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (278.66, 140.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 2192, 3506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9950) [M::mem_pestat] mean and std.dev: (1932.46, 1465.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 342, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744) [M::mem_pestat] mean and std.dev: (592.77, 523.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695532 sequences (100000159 bp) in 2.290 CPU sec, 2.401 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285714, 19, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 295, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (291.61, 151.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (279.78, 141.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (558, 674, 1394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3066) [M::mem_pestat] mean and std.dev: (723.82, 434.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3902) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 337, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1459) [M::mem_pestat] mean and std.dev: (382.55, 306.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695826 reads in 137.685 CPU sec, 4.398 real sec sorting block 168, #reads = 1082213 mem = 536878524 peak = 1122684928 [M::mem_process_seqs] Processed 695556 reads in 88.308 CPU sec, 2.790 real sec [M::process] read 695922 sequences (100000284 bp) in 1.934 CPU sec, 2.015 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285315, 13, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 391, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4231) [M::mem_pestat] mean and std.dev: (897.44, 1136.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 254, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (280.62, 141.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (507, 507, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1707) [M::mem_pestat] mean and std.dev: (585.33, 217.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2107) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 331, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3795) [M::mem_pestat] mean and std.dev: (594.08, 782.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 169, #reads = 1082879 mem = 536876556 peak = 1122684928 [M::process] read 697596 sequences (100000133 bp) in 2.398 CPU sec, 2.502 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 285544, 21, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 323, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013) [M::mem_pestat] mean and std.dev: (533.43, 488.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (279.34, 141.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (427, 733, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3490) [M::mem_pestat] mean and std.dev: (715.11, 483.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4511) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 426, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326) [M::mem_pestat] mean and std.dev: (431.33, 262.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695532 reads in 137.272 CPU sec, 4.376 real sec [M::mem_process_seqs] Processed 695922 reads in 88.169 CPU sec, 2.783 real sec [M::process] read 698870 sequences (100000048 bp) in 1.827 CPU sec, 1.881 real sec... sorting block 170, #reads = 1082730 mem = 536878987 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 286242, 15, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 200, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (292.55, 249.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (273.15, 138.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1068, 1068, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (976, 1206) [M::mem_pestat] mean and std.dev: (1091.00, 23.00) [M::mem_pestat] low and high boundaries for proper pairs: (930, 1252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 372, 1656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4530) [M::mem_pestat] mean and std.dev: (642.94, 705.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697968 sequences (100000044 bp) in 2.469 CPU sec, 2.580 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 287065, 12, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 279, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2305) [M::mem_pestat] mean and std.dev: (435.17, 420.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (267.23, 135.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 414, 2441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6595) [M::mem_pestat] mean and std.dev: (906.64, 892.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 221, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1521) [M::mem_pestat] mean and std.dev: (323.84, 290.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697596 reads in 138.534 CPU sec, 4.412 real sec [M::mem_process_seqs] Processed 698870 reads in 88.386 CPU sec, 2.788 real sec [M::process] read 697388 sequences (100000078 bp) in 1.789 CPU sec, 1.837 real sec... sorting block 171, #reads = 1083745 mem = 536880417 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 286460, 11, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 328, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609) [M::mem_pestat] mean and std.dev: (586.51, 604.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (269.26, 136.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (107, 170, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (124.44, 51.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 238, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (264.30, 117.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696930 sequences (100000177 bp) in 2.496 CPU sec, 2.603 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 286393, 21, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 239, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (208.70, 85.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (272.84, 138.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 1108, 3883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10855) [M::mem_pestat] mean and std.dev: (2286.29, 2746.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 286, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (291.76, 142.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697968 reads in 140.772 CPU sec, 4.481 real sec [M::mem_process_seqs] Processed 697388 reads in 90.379 CPU sec, 2.851 real sec [M::process] read 696968 sequences (100000299 bp) in 1.662 CPU sec, 1.689 real sec... sorting block 172, #reads = 1085758 mem = 536879707 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 286456, 19, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 292, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2214) [M::mem_pestat] mean and std.dev: (463.11, 491.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.53, 139.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1412, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4862) [M::mem_pestat] mean and std.dev: (1101.06, 882.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6283) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 241, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (261.32, 122.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1372) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 173, #reads = 1082310 mem = 536878371 peak = 1122684928 [M::process] read 696662 sequences (100000121 bp) in 2.565 CPU sec, 2.678 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 286108, 28, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 384, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2259) [M::mem_pestat] mean and std.dev: (539.69, 496.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (274.13, 139.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 1205, 3754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10982) [M::mem_pestat] mean and std.dev: (1953.43, 2186.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 221, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (247.81, 117.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696930 reads in 140.496 CPU sec, 4.466 real sec [M::process] read 696050 sequences (100000048 bp) in 1.610 CPU sec, 1.630 real sec... [M::mem_process_seqs] Processed 696968 reads in 90.775 CPU sec, 2.861 real sec sorting block 174, #reads = 1083992 mem = 536881991 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 286055, 13, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 275, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (260.60, 140.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (275.57, 139.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1021, 2481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6123) [M::mem_pestat] mean and std.dev: (1399.08, 979.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 500, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983) [M::mem_pestat] mean and std.dev: (578.49, 478.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695292 sequences (100000144 bp) in 2.597 CPU sec, 2.735 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285715, 19, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 310, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (389.62, 246.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (276.60, 140.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (897, 1871, 3249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7953) [M::mem_pestat] mean and std.dev: (1698.53, 1141.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 313, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (310.34, 145.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696662 reads in 143.728 CPU sec, 4.567 real sec [M::process] read 695006 sequences (100000109 bp) in 1.514 CPU sec, 1.519 real sec... [M::mem_process_seqs] Processed 696050 reads in 92.410 CPU sec, 2.917 real sec sorting block 175, #reads = 1081404 mem = 536879379 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284990, 20, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 185, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (190.42, 70.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (279.37, 141.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1395, 1478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3194) [M::mem_pestat] mean and std.dev: (907.41, 514.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4052) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 403, 834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1978) [M::mem_pestat] mean and std.dev: (493.57, 322.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2550) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695048 sequences (100000240 bp) in 2.572 CPU sec, 2.693 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 284827, 15, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 315, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498) [M::mem_pestat] mean and std.dev: (407.44, 307.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (279.51, 142.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 1441, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4691) [M::mem_pestat] mean and std.dev: (1312.93, 1056.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6166) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1597) [M::mem_pestat] mean and std.dev: (381.41, 292.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695292 reads in 141.200 CPU sec, 4.483 real sec [M::process] read 695176 sequences (100000228 bp) in 1.509 CPU sec, 1.507 real sec... sorting block 176, #reads = 1081234 mem = 536877826 peak = 1122684928 [M::mem_process_seqs] Processed 695006 reads in 91.317 CPU sec, 2.882 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285454, 26, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 260, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1807) [M::mem_pestat] mean and std.dev: (381.16, 342.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (279.48, 141.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (138, 436, 1205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3339) [M::mem_pestat] mean and std.dev: (765.54, 866.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 312, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1467) [M::mem_pestat] mean and std.dev: (371.96, 252.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 177, #reads = 1080589 mem = 536877580 peak = 1122684928 [M::process] read 695302 sequences (100000252 bp) in 2.331 CPU sec, 2.465 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284837, 33, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 276, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1543) [M::mem_pestat] mean and std.dev: (380.49, 275.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (279.31, 141.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (357, 976, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3477) [M::mem_pestat] mean and std.dev: (854.00, 685.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 339, 991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2553) [M::mem_pestat] mean and std.dev: (592.14, 527.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695048 reads in 140.727 CPU sec, 4.475 real sec [M::process] read 696742 sequences (100000092 bp) in 1.618 CPU sec, 1.627 real sec... [M::mem_process_seqs] Processed 695176 reads in 91.072 CPU sec, 2.872 real sec sorting block 178, #reads = 1081906 mem = 536878848 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284919, 17, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 325, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1414) [M::mem_pestat] mean and std.dev: (319.00, 202.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (276.00, 139.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 845, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (616.20, 418.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 296, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (350.07, 215.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695454 sequences (100000279 bp) in 2.201 CPU sec, 2.308 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 285880, 22, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 333, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (311.93, 187.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.48, 137.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (425, 1000, 1333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3149) [M::mem_pestat] mean and std.dev: (846.65, 627.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 269, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (284.38, 169.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695942 sequences (100000213 bp) in 1.500 CPU sec, 1.494 real sec... [M::mem_process_seqs] Processed 695302 reads in 150.974 CPU sec, 4.805 real sec [M::mem_process_seqs] Processed 696742 reads in 98.952 CPU sec, 3.132 real sec sorting block 179, #reads = 1078828 mem = 536882197 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 285414, 12, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 254, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (284.03, 188.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (275.63, 138.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (255, 932, 2770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7800) [M::mem_pestat] mean and std.dev: (1231.92, 1303.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 409, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3168) [M::mem_pestat] mean and std.dev: (585.14, 620.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695220 sequences (100000114 bp) in 2.619 CPU sec, 2.759 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 285029, 22, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 215, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (270.77, 174.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.70, 138.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 582, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2888) [M::mem_pestat] mean and std.dev: (613.84, 760.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 436, 1102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2864) [M::mem_pestat] mean and std.dev: (493.12, 416.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695178 sequences (100000092 bp) in 1.490 CPU sec, 1.492 real sec... [M::mem_process_seqs] Processed 695454 reads in 147.699 CPU sec, 4.688 real sec [M::mem_process_seqs] Processed 695942 reads in 94.253 CPU sec, 2.980 real sec sorting block 180, #reads = 1077583 mem = 536878151 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284726, 13, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 285, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1440) [M::mem_pestat] mean and std.dev: (356.69, 283.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.57, 140.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1132, 1302, 1958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3610) [M::mem_pestat] mean and std.dev: (1404.85, 756.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 277, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444) [M::mem_pestat] mean and std.dev: (358.93, 290.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1859) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695184 sequences (100000281 bp) in 2.352 CPU sec, 2.462 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284681, 18, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 215, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (217.02, 102.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.66, 141.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 765, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3773) [M::mem_pestat] mean and std.dev: (861.61, 818.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 396, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1967) [M::mem_pestat] mean and std.dev: (444.48, 342.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2552) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695920 sequences (100000028 bp) in 1.500 CPU sec, 1.497 real sec... [M::mem_process_seqs] Processed 695220 reads in 148.791 CPU sec, 4.742 real sec sorting block 181, #reads = 1079300 mem = 536878294 peak = 1122684928 [M::mem_process_seqs] Processed 695178 reads in 93.891 CPU sec, 2.980 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284895, 17, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 344, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1500) [M::mem_pestat] mean and std.dev: (343.67, 212.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.86, 140.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (489, 1195, 6149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17469) [M::mem_pestat] mean and std.dev: (2426.53, 2524.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23129) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 264, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1813) [M::mem_pestat] mean and std.dev: (436.25, 427.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2363) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 182, #reads = 1080313 mem = 536879036 peak = 1122684928 [M::process] read 695452 sequences (100000272 bp) in 2.383 CPU sec, 2.480 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285794, 16, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 314, 908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2382) [M::mem_pestat] mean and std.dev: (506.33, 451.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.69, 140.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (686, 1266, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3242) [M::mem_pestat] mean and std.dev: (1131.44, 741.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4096) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 330, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (343.47, 184.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695392 sequences (100000279 bp) in 1.520 CPU sec, 1.523 real sec... [M::mem_process_seqs] Processed 695184 reads in 147.590 CPU sec, 4.690 real sec [M::mem_process_seqs] Processed 695920 reads in 96.291 CPU sec, 3.048 real sec sorting block 183, #reads = 1080189 mem = 536880559 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 284982, 12, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 250, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277) [M::mem_pestat] mean and std.dev: (315.43, 229.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (276.06, 140.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (447, 748, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2316) [M::mem_pestat] mean and std.dev: (629.64, 380.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2939) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 264, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1699) [M::mem_pestat] mean and std.dev: (386.17, 325.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695370 sequences (100000013 bp) in 2.424 CPU sec, 2.545 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 285534, 13, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 322, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2162) [M::mem_pestat] mean and std.dev: (453.32, 440.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.90, 140.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 543, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4683) [M::mem_pestat] mean and std.dev: (1209.08, 1191.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6141) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 336, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1650) [M::mem_pestat] mean and std.dev: (460.71, 379.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695886 sequences (100000273 bp) in 1.530 CPU sec, 1.530 real sec... [M::mem_process_seqs] Processed 695452 reads in 147.116 CPU sec, 4.675 real sec [M::mem_process_seqs] Processed 695392 reads in 94.976 CPU sec, 3.001 real sec sorting block 184, #reads = 1079605 mem = 536885914 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285329, 18, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 231, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (264.31, 202.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (275.36, 139.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (330, 1585, 1925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5115) [M::mem_pestat] mean and std.dev: (1228.76, 1044.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 243, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1638) [M::mem_pestat] mean and std.dev: (366.02, 295.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695196 sequences (100000197 bp) in 2.381 CPU sec, 2.489 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285210, 24, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 242, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1643) [M::mem_pestat] mean and std.dev: (330.20, 265.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (272.29, 137.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (344, 531, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2564) [M::mem_pestat] mean and std.dev: (641.77, 527.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 428, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (455.22, 312.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1937) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699104 sequences (100000069 bp) in 1.560 CPU sec, 1.553 real sec... [M::mem_process_seqs] Processed 695370 reads in 152.333 CPU sec, 4.839 real sec sorting block 185, #reads = 1079244 mem = 536877852 peak = 1122684928 [M::mem_process_seqs] Processed 695886 reads in 98.987 CPU sec, 3.133 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284440, 13, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 292, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1673) [M::mem_pestat] mean and std.dev: (385.24, 364.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (275.06, 138.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (149, 506, 2580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7442) [M::mem_pestat] mean and std.dev: (1021.69, 1121.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 318, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (289.88, 127.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 186, #reads = 1077911 mem = 536880431 peak = 1122684928 [M::process] read 704824 sequences (100000213 bp) in 2.517 CPU sec, 2.650 real sec... [M::process] read 702548 sequences (100000170 bp) in 1.570 CPU sec, 1.571 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 285970, 10, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 328, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (324.60, 173.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 237, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (264.76, 134.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (267, 522, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419) [M::mem_pestat] mean and std.dev: (346.25, 216.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 358, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358) [M::mem_pestat] mean and std.dev: (411.17, 319.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695196 reads in 166.327 CPU sec, 5.291 real sec [M::mem_process_seqs] Processed 699104 reads in 108.892 CPU sec, 3.452 real sec sorting block 187, #reads = 1070191 mem = 536879189 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 288433, 14, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 307, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2751) [M::mem_pestat] mean and std.dev: (514.46, 491.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (251.20, 128.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (508, 864, 4144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11416) [M::mem_pestat] mean and std.dev: (2006.00, 2011.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15052) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458) [M::mem_pestat] mean and std.dev: (338.38, 265.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700836 sequences (100000194 bp) in 2.499 CPU sec, 2.605 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 287876, 15, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 287, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (313.60, 228.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (257.67, 131.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (370, 696, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1987) [M::mem_pestat] mean and std.dev: (612.27, 381.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 256, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (244.71, 130.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699806 sequences (100000191 bp) in 1.550 CPU sec, 1.550 real sec... [M::mem_process_seqs] Processed 704824 reads in 156.555 CPU sec, 4.970 real sec [M::mem_process_seqs] Processed 702548 reads in 99.743 CPU sec, 3.162 real sec sorting block 188, #reads = 1078477 mem = 536879466 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 287534, 19, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 405, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2990) [M::mem_pestat] mean and std.dev: (737.45, 821.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (262.94, 135.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 243, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (310.59, 286.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 353, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4799) [M::mem_pestat] mean and std.dev: (753.17, 979.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699654 sequences (100000266 bp) in 2.486 CPU sec, 2.632 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 287240, 20, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 194, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1909) [M::mem_pestat] mean and std.dev: (384.27, 429.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (267.17, 137.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 796, 1981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5143) [M::mem_pestat] mean and std.dev: (1000.11, 1130.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6724) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 380, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1787) [M::mem_pestat] mean and std.dev: (492.62, 373.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700836 reads in 150.031 CPU sec, 4.770 real sec [M::process] read 698588 sequences (100000242 bp) in 1.589 CPU sec, 1.590 real sec... [M::mem_process_seqs] Processed 699806 reads in 95.965 CPU sec, 3.029 real sec sorting block 189, #reads = 1079585 mem = 536879113 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 287162, 25, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 257, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1920) [M::mem_pestat] mean and std.dev: (439.12, 441.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (268.11, 137.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (120, 302, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2712) [M::mem_pestat] mean and std.dev: (465.09, 458.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 337, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (374.37, 159.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 190, #reads = 1081928 mem = 536879525 peak = 1122684928 [M::process] read 697282 sequences (100000076 bp) in 2.411 CPU sec, 2.521 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 286750, 17, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 430, 1569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4315) [M::mem_pestat] mean and std.dev: (864.46, 953.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.01, 138.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (931, 1072, 2400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5338) [M::mem_pestat] mean and std.dev: (1353.18, 803.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 309, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (501.42, 421.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698590 sequences (100000051 bp) in 1.550 CPU sec, 1.549 real sec... [M::mem_process_seqs] Processed 699654 reads in 148.963 CPU sec, 4.734 real sec [M::mem_process_seqs] Processed 698588 reads in 95.268 CPU sec, 3.008 real sec sorting block 191, #reads = 1081604 mem = 536881145 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 286366, 19, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 390, 1522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4200) [M::mem_pestat] mean and std.dev: (865.86, 1090.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (274.70, 139.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 612, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4759) [M::mem_pestat] mean and std.dev: (894.61, 926.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 267, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (308.65, 218.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696494 sequences (100000169 bp) in 2.610 CPU sec, 2.744 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286385, 13, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 205, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1873) [M::mem_pestat] mean and std.dev: (406.79, 418.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (269.92, 137.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 384, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4326) [M::mem_pestat] mean and std.dev: (762.15, 706.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 495, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2184) [M::mem_pestat] mean and std.dev: (461.81, 261.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697282 reads in 148.077 CPU sec, 4.702 real sec [M::process] read 696318 sequences (100000023 bp) in 1.738 CPU sec, 1.762 real sec... [M::mem_process_seqs] Processed 698590 reads in 94.800 CPU sec, 2.990 real sec sorting block 192, #reads = 1080337 mem = 536878270 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 286226, 19, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 296, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443) [M::mem_pestat] mean and std.dev: (370.18, 230.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (276.96, 140.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (315, 846, 3004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8382) [M::mem_pestat] mean and std.dev: (1345.44, 1626.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11071) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 322, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (328.10, 154.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696230 sequences (100000040 bp) in 2.341 CPU sec, 2.479 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 286232, 10, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 227, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (274.32, 147.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (278.04, 141.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (124, 483, 1989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5719) [M::mem_pestat] mean and std.dev: (881.20, 907.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 328, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (286.48, 154.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695386 sequences (100000126 bp) in 1.570 CPU sec, 1.570 real sec... [M::mem_process_seqs] Processed 696494 reads in 151.689 CPU sec, 4.880 real sec [M::mem_process_seqs] Processed 696318 reads in 94.671 CPU sec, 2.989 real sec sorting block 193, #reads = 1080873 mem = 536878464 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285850, 25, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 415, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153) [M::mem_pestat] mean and std.dev: (339.31, 240.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (278.72, 141.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (447, 733, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4965) [M::mem_pestat] mean and std.dev: (1085.80, 844.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 297, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1305) [M::mem_pestat] mean and std.dev: (343.14, 268.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696262 sequences (100000111 bp) in 2.422 CPU sec, 2.550 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285610, 25, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 264, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1895) [M::mem_pestat] mean and std.dev: (414.00, 320.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 254, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (281.33, 142.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (133, 711, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4270) [M::mem_pestat] mean and std.dev: (797.92, 634.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 338, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3496) [M::mem_pestat] mean and std.dev: (563.33, 501.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696892 sequences (100000093 bp) in 1.540 CPU sec, 1.543 real sec... [M::mem_process_seqs] Processed 696230 reads in 146.500 CPU sec, 4.654 real sec sorting block 194, #reads = 1080172 mem = 536878539 peak = 1122684928 [M::mem_process_seqs] Processed 695386 reads in 93.245 CPU sec, 2.945 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 285705, 18, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 262, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217) [M::mem_pestat] mean and std.dev: (246.96, 160.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 195, #reads = 1079962 mem = 536882498 peak = 1122684928 [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (277.68, 141.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 1012, 1657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4529) [M::mem_pestat] mean and std.dev: (818.40, 783.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 319, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (326.30, 192.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696912 sequences (100000132 bp) in 2.302 CPU sec, 2.402 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 285715, 17, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1462) [M::mem_pestat] mean and std.dev: (300.37, 223.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (273.99, 138.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (126, 479, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3249) [M::mem_pestat] mean and std.dev: (665.47, 636.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4290) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 428, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (421.10, 205.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695540 sequences (100000215 bp) in 1.510 CPU sec, 1.514 real sec... [M::mem_process_seqs] Processed 696262 reads in 145.046 CPU sec, 4.612 real sec [M::mem_process_seqs] Processed 696892 reads in 93.111 CPU sec, 2.942 real sec sorting block 196, #reads = 1080664 mem = 536879000 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 285875, 23, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 284, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (302.64, 173.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.32, 136.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 444, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1892) [M::mem_pestat] mean and std.dev: (479.14, 306.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 359, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2904) [M::mem_pestat] mean and std.dev: (605.82, 640.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695296 sequences (100000126 bp) in 2.309 CPU sec, 2.531 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285675, 23, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 248, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240) [M::mem_pestat] mean and std.dev: (264.26, 153.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.94, 138.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (719, 1113, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3170) [M::mem_pestat] mean and std.dev: (867.79, 476.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 343, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (383.54, 246.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694852 sequences (100000175 bp) in 1.520 CPU sec, 1.514 real sec... [M::mem_process_seqs] Processed 696912 reads in 144.654 CPU sec, 4.602 real sec [M::mem_process_seqs] Processed 695540 reads in 100.592 CPU sec, 3.205 real sec sorting block 197, #reads = 1081555 mem = 536879646 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 285591, 22, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 361, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1649) [M::mem_pestat] mean and std.dev: (374.29, 229.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (277.71, 139.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (428, 939, 1705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4259) [M::mem_pestat] mean and std.dev: (1085.05, 911.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 375, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138) [M::mem_pestat] mean and std.dev: (307.13, 169.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694764 sequences (100000081 bp) in 2.406 CPU sec, 2.533 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 284909, 22, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 270, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1762) [M::mem_pestat] mean and std.dev: (451.46, 417.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (278.59, 139.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 504, 1476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3628) [M::mem_pestat] mean and std.dev: (1032.59, 1089.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (323, 548, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2492) [M::mem_pestat] mean and std.dev: (637.31, 474.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694850 sequences (100000059 bp) in 1.510 CPU sec, 1.517 real sec... [M::mem_process_seqs] Processed 695296 reads in 145.793 CPU sec, 4.633 real sec sorting block 198, #reads = 1080444 mem = 536878511 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (102, 285062, 25, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1154) [M::mem_pestat] mean and std.dev: (315.21, 216.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (279.00, 139.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 524, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1786) [M::mem_pestat] mean and std.dev: (609.62, 458.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 276, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (266.44, 107.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694946 sequences (100000296 bp) in 1.817 CPU sec, 1.822 real sec... [M::mem_process_seqs] Processed 694764 reads in 86.051 CPU sec, 2.732 real sec sorting block 199, #reads = 1079666 mem = 536877423 peak = 1122684928 [M::mem_process_seqs] Processed 694852 reads in 200.371 CPU sec, 6.361 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284835, 32, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 350, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (284.39, 128.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (278.16, 138.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 1233, 2149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6045) [M::mem_pestat] mean and std.dev: (1526.74, 1629.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 367, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (391.73, 248.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694354 sequences (100000119 bp) in 2.481 CPU sec, 2.588 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284379, 23, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 312, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1474) [M::mem_pestat] mean and std.dev: (382.21, 311.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (275.38, 135.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 738, 8515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25077) [M::mem_pestat] mean and std.dev: (3275.70, 3801.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 33358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 297, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2225) [M::mem_pestat] mean and std.dev: (450.79, 433.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694850 reads in 166.034 CPU sec, 5.266 real sec [M::process] read 693862 sequences (100000206 bp) in 1.603 CPU sec, 1.617 real sec... [M::mem_process_seqs] Processed 694946 reads in 99.312 CPU sec, 3.133 real sec sorting block 200, #reads = 1079525 mem = 536880114 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 285162, 21, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 252, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (263.11, 109.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (277.20, 136.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 879, 2162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6066) [M::mem_pestat] mean and std.dev: (1136.90, 968.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 355, 1718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4738) [M::mem_pestat] mean and std.dev: (837.12, 956.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 201, #reads = 1076183 mem = 536883087 peak = 1122684928 [M::process] read 693908 sequences (100000017 bp) in 2.487 CPU sec, 2.643 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284617, 18, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 389, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452) [M::mem_pestat] mean and std.dev: (387.65, 250.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (278.00, 136.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (416, 960, 2306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6086) [M::mem_pestat] mean and std.dev: (1422.50, 1134.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 357, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (346.49, 130.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693820 sequences (100000197 bp) in 1.500 CPU sec, 1.500 real sec... [M::mem_process_seqs] Processed 694354 reads in 152.876 CPU sec, 4.871 real sec [M::mem_process_seqs] Processed 693862 reads in 95.021 CPU sec, 3.004 real sec sorting block 202, #reads = 1079041 mem = 536880416 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284534, 20, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 292, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (316.38, 174.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (277.35, 135.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 512, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2499) [M::mem_pestat] mean and std.dev: (641.37, 695.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 241, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349) [M::mem_pestat] mean and std.dev: (350.70, 284.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694486 sequences (100000190 bp) in 2.310 CPU sec, 2.437 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 284724, 26, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 223, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550) [M::mem_pestat] mean and std.dev: (342.71, 314.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (277.16, 135.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 529, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3065) [M::mem_pestat] mean and std.dev: (676.38, 616.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4022) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 312, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (303.38, 144.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693470 sequences (100000263 bp) in 1.480 CPU sec, 1.488 real sec... [M::mem_process_seqs] Processed 693908 reads in 152.267 CPU sec, 4.921 real sec [M::mem_process_seqs] Processed 693820 reads in 96.059 CPU sec, 3.051 real sec sorting block 203, #reads = 1079690 mem = 536883697 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 284697, 20, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 382, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2078) [M::mem_pestat] mean and std.dev: (477.05, 347.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 252, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (275.81, 135.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (366, 1456, 2197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5859) [M::mem_pestat] mean and std.dev: (1514.89, 1551.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 347, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389) [M::mem_pestat] mean and std.dev: (369.94, 252.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693568 sequences (100000149 bp) in 2.369 CPU sec, 2.492 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284596, 31, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 321, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (363.25, 245.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (277.57, 135.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (107, 403, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2294) [M::mem_pestat] mean and std.dev: (362.00, 338.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 342, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (381.05, 274.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693150 sequences (100000163 bp) in 1.490 CPU sec, 1.483 real sec... [M::mem_process_seqs] Processed 694486 reads in 146.343 CPU sec, 4.656 real sec [M::mem_process_seqs] Processed 693470 reads in 93.127 CPU sec, 2.944 real sec sorting block 204, #reads = 1079816 mem = 536881203 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284178, 16, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 271, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (366.44, 277.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (276.32, 134.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (723, 1883, 2853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7113) [M::mem_pestat] mean and std.dev: (1613.20, 1091.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 389, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1932) [M::mem_pestat] mean and std.dev: (489.17, 431.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693990 sequences (100000147 bp) in 2.428 CPU sec, 2.572 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283981, 20, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 268, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1241) [M::mem_pestat] mean and std.dev: (375.12, 272.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 254, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (276.99, 135.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (671, 1348, 2677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6689) [M::mem_pestat] mean and std.dev: (1672.00, 1519.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 296, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767) [M::mem_pestat] mean and std.dev: (415.58, 349.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693850 sequences (100000027 bp) in 1.480 CPU sec, 1.485 real sec... [M::mem_process_seqs] Processed 693568 reads in 150.101 CPU sec, 4.795 real sec sorting block 205, #reads = 1079806 mem = 536877616 peak = 1122684928 [M::mem_process_seqs] Processed 693150 reads in 92.662 CPU sec, 2.936 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 283421, 23, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 277, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2548) [M::mem_pestat] mean and std.dev: (386.20, 375.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 251, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (274.93, 134.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 560, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1921) [M::mem_pestat] mean and std.dev: (561.82, 379.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 251, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (281.00, 136.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 206, #reads = 1078407 mem = 536880537 peak = 1122684928 [M::process] read 693172 sequences (100000111 bp) in 2.451 CPU sec, 2.567 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284253, 28, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 262, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1553) [M::mem_pestat] mean and std.dev: (320.31, 255.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 252, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (276.08, 135.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 771, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4907) [M::mem_pestat] mean and std.dev: (1099.25, 1165.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 350, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454) [M::mem_pestat] mean and std.dev: (397.95, 253.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693072 sequences (100000101 bp) in 1.480 CPU sec, 1.474 real sec... [M::mem_process_seqs] Processed 693990 reads in 145.073 CPU sec, 4.614 real sec [M::mem_process_seqs] Processed 693850 reads in 92.664 CPU sec, 2.933 real sec sorting block 207, #reads = 1080100 mem = 536879131 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 284042, 21, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 260, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (283.64, 175.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 254, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (277.09, 135.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 635, 2005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5725) [M::mem_pestat] mean and std.dev: (930.90, 850.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 431, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (675.70, 650.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693292 sequences (100000151 bp) in 2.446 CPU sec, 2.575 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 284088, 19, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 271, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (280.76, 136.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (278.25, 135.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (742, 1033, 2971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7429) [M::mem_pestat] mean and std.dev: (1792.11, 1934.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9658) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 355, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (316.02, 167.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693172 reads in 143.826 CPU sec, 4.574 real sec [M::process] read 693204 sequences (100000078 bp) in 1.509 CPU sec, 1.512 real sec... [M::mem_process_seqs] Processed 693072 reads in 92.408 CPU sec, 2.917 real sec sorting block 208, #reads = 1079922 mem = 536882778 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 284054, 15, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 281, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (258.86, 124.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 255, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (278.26, 135.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (198, 1213, 2971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8517) [M::mem_pestat] mean and std.dev: (1835.80, 1787.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11290) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 336, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2773) [M::mem_pestat] mean and std.dev: (593.23, 497.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693006 sequences (100000200 bp) in 2.438 CPU sec, 2.561 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284427, 15, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 221, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (260.64, 174.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 254, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (277.14, 135.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (227, 319, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2594) [M::mem_pestat] mean and std.dev: (549.57, 550.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3383) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 214, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1479) [M::mem_pestat] mean and std.dev: (280.02, 208.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693292 reads in 141.470 CPU sec, 4.502 real sec [M::process] read 693298 sequences (100000049 bp) in 1.566 CPU sec, 1.563 real sec... [M::mem_process_seqs] Processed 693204 reads in 90.296 CPU sec, 2.844 real sec sorting block 209, #reads = 1080956 mem = 536880345 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284235, 26, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 234, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (222.27, 88.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 255, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (278.47, 136.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 1480, 2531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7103) [M::mem_pestat] mean and std.dev: (1963.46, 1974.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 256, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (267.31, 118.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 210, #reads = 1080390 mem = 536880450 peak = 1122684928 [M::process] read 693146 sequences (100000138 bp) in 2.481 CPU sec, 2.608 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 284307, 15, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 216, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (277.41, 168.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (278.83, 137.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (519, 610, 5243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14691) [M::mem_pestat] mean and std.dev: (2304.93, 3137.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 334, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483) [M::mem_pestat] mean and std.dev: (537.57, 490.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693006 reads in 140.791 CPU sec, 4.473 real sec [M::process] read 693342 sequences (100000221 bp) in 1.644 CPU sec, 1.663 real sec... [M::mem_process_seqs] Processed 693298 reads in 90.468 CPU sec, 2.852 real sec sorting block 211, #reads = 1080633 mem = 536877298 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 284018, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 334, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (311.68, 122.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (279.81, 138.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (700, 1384, 3370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8710) [M::mem_pestat] mean and std.dev: (1926.75, 1805.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 237, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (318.67, 253.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 444224 sequences (63984235 bp) in 1.868 CPU sec, 1.980 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 284705, 10, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 339, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794) [M::mem_pestat] mean and std.dev: (420.60, 305.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (279.73, 138.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 1346, 1675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4405) [M::mem_pestat] mean and std.dev: (1092.30, 717.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 368, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (420.44, 257.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693146 reads in 140.900 CPU sec, 4.542 real sec [M::mem_process_seqs] Processed 693342 reads in 90.209 CPU sec, 2.910 real sec sorting block 212, #reads = 1079119 mem = 536879539 peak = 1122684928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 181804, 14, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 398, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1384) [M::mem_pestat] mean and std.dev: (499.62, 291.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (275.69, 136.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 1362, 3633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10355) [M::mem_pestat] mean and std.dev: (2510.79, 2810.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 368, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502) [M::mem_pestat] mean and std.dev: (376.76, 182.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 444224 reads in 54.128 CPU sec, 1.801 real sec sorting block 213, #reads = 1078693 mem = 536879751 peak = 1165729792 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 229937742 overall: 70109253632 mem 30225.722 user 688.314 sys 1012.740 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_5TB-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:1136_5TB-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_5TB-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_5TB-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_5TB-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 1012.777 sec; CPU: 30914.039 sec sorting block 214, #reads = 114389 mem = 57210566 peak = 1165729792 uniq header count 1 merging 215 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 12031616438 output reads: 231436866 bam_mem_sort: 215 calls 110.257 user 2.177 sys 112.865 real bam_write: 215 calls 67.479 user 4.062 sys 79.584 real execute: 1 calls 45.028 user 2.685 sys 1076.998 real merge_files: 1 calls 45.008 user 2.665 sys 64.038 real parse_chunk: 3426 calls 134.600 user 34.724 sys 173.796 real read_chunk: 9255 calls 79.644 user 79.945 sys 958.656 real sort_block: 1 calls 55.683 user 79.068 sys 925.408 real write_chunk: 5827 calls 44.632 user 2.276 sys 62.967 real overall: 3636510720 mem 2622.209 user 145.663 sys 1077.017 real ls: cannot access '*.cram': No such file or directory