.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L005;0004)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/03/202db4d8dbe9a5c78144cc5a364c28/.command.err
Size: 132.6 KB
Task: DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L005;0004)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
Received only 12 licenses out of 31 requested for sentieon:klib
Received only 12 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 1269.200 CPU sec, 586.477 real sec
[M::process] read 703778 sequences (100000061 bp) in 1.009 CPU sec, 1.012 real sec...
[M::process] read 701702 sequences (100000127 bp) in 1.075 CPU sec, 1.082 real sec...
[M::process] read 701444 sequences (100000054 bp) in 1.105 CPU sec, 1.146 real sec...
[M::process] read 701378 sequences (100000291 bp) in 1.134 CPU sec, 1.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292528, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (234.42, 109.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291733, 9, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.16, 115.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 451, 945)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413)
[M::mem_pestat] mean and std.dev: (602.00, 476.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3147)
[M::mem_pestat] skip orientation RR
Received only 12 licenses out of 31 requested for sentieon:klib
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291918, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (239.72, 112.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703778 reads in 173.595 CPU sec, 14.551 real sec
[M::mem_process_seqs] Processed 701444 reads in 127.574 CPU sec, 10.908 real sec
sorting block 0, #reads = 1095673 mem = 536879655 peak = 586067968
[M::mem_process_seqs] Processed 701702 reads in 141.142 CPU sec, 12.006 real sec
[M::process] read 700500 sequences (100000007 bp) in 1.550 CPU sec, 1.601 real sec...
[M::process] read 700014 sequences (100000075 bp) in 1.447 CPU sec, 1.490 real sec...
[M::process] read 699220 sequences (100000236 bp) in 1.060 CPU sec, 1.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292066, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.84, 117.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291503, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (246.79, 118.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701378 reads in 118.780 CPU sec, 9.749 real sec
sorting block 1, #reads = 1095313 mem = 536879881 peak = 932671488
[M::process] read 698856 sequences (100000243 bp) in 1.269 CPU sec, 1.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291341, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.54, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699220 reads in 66.156 CPU sec, 5.451 real sec
sorting block 2, #reads = 1095826 mem = 536877009 peak = 932671488
[M::mem_process_seqs] Processed 700500 reads in 148.690 CPU sec, 12.376 real sec
[M::process] read 698896 sequences (100000098 bp) in 2.357 CPU sec, 2.597 real sec...
[M::process] read 698192 sequences (100000074 bp) in 1.170 CPU sec, 1.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291281, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (247, 521, 1760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4786)
[M::mem_pestat] mean and std.dev: (844.60, 847.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6299)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.19, 119.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291783, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (251.90, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290904, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.08, 121.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 2323, 2660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7462)
[M::mem_pestat] mean and std.dev: (1464.55, 1132.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9863)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700014 reads in 239.583 CPU sec, 19.713 real sec
sorting block 3, #reads = 1095193 mem = 536877215 peak = 932671488
[M::mem_process_seqs] Processed 698856 reads in 128.265 CPU sec, 10.746 real sec
[M::mem_process_seqs] Processed 698896 reads in 141.848 CPU sec, 11.853 real sec
sorting block 4, #reads = 1094652 mem = 536880730 peak = 961462272
[M::process] read 697520 sequences (100000138 bp) in 1.928 CPU sec, 2.030 real sec...
[M::process] read 696308 sequences (100000213 bp) in 1.809 CPU sec, 1.946 real sec...
[M::process] read 696412 sequences (100000291 bp) in 1.160 CPU sec, 1.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291355, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.30, 121.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290340, 6, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 292, 1206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3262)
[M::mem_pestat] mean and std.dev: (697.83, 703.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4290)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (255.64, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290609, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.67, 121.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698192 reads in 168.738 CPU sec, 13.871 real sec
sorting block 5, #reads = 1093980 mem = 536881183 peak = 961462272
[M::mem_process_seqs] Processed 696308 reads in 123.959 CPU sec, 10.377 real sec
[M::mem_process_seqs] Processed 697520 reads in 137.216 CPU sec, 11.463 real sec
sorting block 6, #reads = 1092635 mem = 536880934 peak = 961462272
[M::process] read 696418 sequences (100000007 bp) in 2.461 CPU sec, 2.709 real sec...
[M::process] read 696258 sequences (100000206 bp) in 1.804 CPU sec, 1.869 real sec...
[M::process] read 696168 sequences (100000072 bp) in 1.250 CPU sec, 1.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290126, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.62, 120.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
Received only 12 licenses out of 31 requested for sentieon:klib
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290177, 6, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.25, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 390, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (362.00, 141.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696412 reads in 122.733 CPU sec, 10.058 real sec
sorting block 7, #reads = 1092640 mem = 536879587 peak = 961462272
[M::process] read 696472 sequences (100000269 bp) in 1.599 CPU sec, 1.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289984, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (252.42, 117.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696418 reads in 130.072 CPU sec, 10.744 real sec
[M::mem_process_seqs] Processed 696258 reads in 145.012 CPU sec, 12.034 real sec
sorting block 8, #reads = 1090667 mem = 536880238 peak = 961462272
[M::process] read 699278 sequences (100000248 bp) in 2.570 CPU sec, 2.819 real sec...
[M::process] read 702834 sequences (100000254 bp) in 1.290 CPU sec, 1.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290036, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (347, 388, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (239, 509)
[M::mem_pestat] mean and std.dev: (385.50, 18.17)
[M::mem_pestat] low and high boundaries for proper pairs: (185, 563)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.50, 120.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290522, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.37, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 276, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (171, 356)
[M::mem_pestat] mean and std.dev: (256.71, 28.27)
[M::mem_pestat] low and high boundaries for proper pairs: (134, 393)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696472 reads in 124.351 CPU sec, 10.157 real sec
sorting block 9, #reads = 1090082 mem = 536879244 peak = 961462272
[M::mem_process_seqs] Processed 696168 reads in 137.435 CPU sec, 11.282 real sec
[M::process] read 701616 sequences (100000037 bp) in 1.776 CPU sec, 1.877 real sec...
[M::process] read 701246 sequences (100000181 bp) in 1.270 CPU sec, 1.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292303, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.93, 115.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701616 reads in 61.281 CPU sec, 5.259 real sec
sorting block 10, #reads = 1090329 mem = 536879839 peak = 995422208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291313, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 265, 847)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2149)
[M::mem_pestat] mean and std.dev: (486.45, 486.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.89, 118.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700768 sequences (100000088 bp) in 1.545 CPU sec, 1.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292396, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (237.85, 112.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699278 reads in 183.312 CPU sec, 15.122 real sec
sorting block 11, #reads = 1093211 mem = 536878770 peak = 998010880
[M::process] read 700066 sequences (100000129 bp) in 1.470 CPU sec, 1.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291747, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.96, 116.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701246 reads in 62.158 CPU sec, 5.125 real sec
[M::mem_process_seqs] Processed 702834 reads in 212.523 CPU sec, 17.754 real sec
sorting block 12, #reads = 1096108 mem = 536880055 peak = 998010880
[M::process] read 700098 sequences (100000098 bp) in 2.334 CPU sec, 2.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291662, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.47, 116.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699010 sequences (100000117 bp) in 1.350 CPU sec, 1.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291727, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.10, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700768 reads in 134.004 CPU sec, 11.005 real sec
sorting block 13, #reads = 1095720 mem = 536883493 peak = 998010880
[M::mem_process_seqs] Processed 700066 reads in 79.963 CPU sec, 6.696 real sec
[M::process] read 698798 sequences (100000073 bp) in 2.508 CPU sec, 2.736 real sec...
[M::process] read 698300 sequences (100000244 bp) in 1.630 CPU sec, 1.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291513, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.04, 119.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
Received only 12 licenses out of 31 requested for sentieon:klib
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291311, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.12, 118.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700098 reads in 122.894 CPU sec, 9.998 real sec
sorting block 14, #reads = 1096037 mem = 536877776 peak = 998010880
[M::mem_process_seqs] Processed 699010 reads in 135.387 CPU sec, 11.163 real sec
sorting block 15, #reads = 1094794 mem = 536878958 peak = 1001525248
[M::process] read 698398 sequences (100000185 bp) in 2.387 CPU sec, 2.514 real sec...
[M::process] read 697844 sequences (100000178 bp) in 1.730 CPU sec, 1.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291594, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.09, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290722, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.58, 119.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698300 reads in 124.038 CPU sec, 10.194 real sec
[M::mem_process_seqs] Processed 698798 reads in 135.300 CPU sec, 11.156 real sec
sorting block 16, #reads = 1095457 mem = 536877835 peak = 1001525248
[M::process] read 696874 sequences (100000230 bp) in 2.386 CPU sec, 2.528 real sec...
[M::process] read 697182 sequences (100000036 bp) in 1.820 CPU sec, 1.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290775, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 197, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (173.10, 46.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.66, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290287, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.34, 119.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698398 reads in 125.623 CPU sec, 10.205 real sec
[M::process] read 697234 sequences (100000045 bp) in 2.158 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290686, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (252.89, 120.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696874 reads in 134.571 CPU sec, 11.059 real sec
sorting block 17, #reads = 1093317 mem = 536882202 peak = 1001525248
[M::mem_process_seqs] Processed 697844 reads in 148.656 CPU sec, 12.230 real sec
sorting block 18, #reads = 1092557 mem = 536881451 peak = 1073442816
[M::process] read 696940 sequences (100000155 bp) in 2.582 CPU sec, 2.698 real sec...
[M::process] read 696682 sequences (100000259 bp) in 1.640 CPU sec, 1.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290614, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.84, 119.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290581, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 162, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (222.71, 125.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.69, 118.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 1605, 1940)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5268)
[M::mem_pestat] mean and std.dev: (821.88, 741.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6932)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697182 reads in 120.557 CPU sec, 9.794 real sec
[M::process] read 696586 sequences (100000105 bp) in 2.283 CPU sec, 2.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290261, 11, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.04, 118.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (382, 642, 700)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336)
[M::mem_pestat] mean and std.dev: (430.56, 280.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1654)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 265, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533)
[M::mem_pestat] mean and std.dev: (291.89, 188.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1986)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696940 reads in 66.032 CPU sec, 5.353 real sec
sorting block 19, #reads = 1092874 mem = 536879183 peak = 1073442816
[M::mem_process_seqs] Processed 697234 reads in 207.678 CPU sec, 16.902 real sec
sorting block 20, #reads = 1092113 mem = 536883357 peak = 1073442816
[M::process] read 700914 sequences (100000266 bp) in 2.628 CPU sec, 2.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290385, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.39, 120.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702556 sequences (100000083 bp) in 1.650 CPU sec, 1.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291858, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.41, 114.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 304, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (290.27, 92.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290212, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.37, 121.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696682 reads in 252.558 CPU sec, 18.603 real sec
[M::mem_process_seqs] Processed 700914 reads in 137.687 CPU sec, 8.887 real sec
[M::mem_process_seqs] Processed 696586 reads in 151.916 CPU sec, 9.523 real sec
sorting block 21, #reads = 1091576 mem = 536878937 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291931, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (238.58, 112.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702556 reads in 74.797 CPU sec, 2.541 real sec
[M::process] read 700716 sequences (100000045 bp) in 2.484 CPU sec, 2.675 real sec...
sorting block 22, #reads = 1093589 mem = 536880410 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291339, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.11, 115.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700716 reads in 74.434 CPU sec, 2.459 real sec
[M::process] read 700250 sequences (100000146 bp) in 2.435 CPU sec, 2.643 real sec...
sorting block 23, #reads = 1095130 mem = 536880385 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291223, 6, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 469, 1012)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2654)
[M::mem_pestat] mean and std.dev: (692.10, 568.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.65, 117.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700250 reads in 73.092 CPU sec, 2.402 real sec
[M::process] read 699854 sequences (100000133 bp) in 2.717 CPU sec, 2.954 real sec...
sorting block 24, #reads = 1093632 mem = 536878249 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291660, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.17, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 297, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (252.40, 95.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RR
[M::process] read 699716 sequences (100000233 bp) in 2.205 CPU sec, 2.326 real sec...
[M::mem_process_seqs] Processed 699854 reads in 73.772 CPU sec, 2.386 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291991, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.11, 120.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699716 reads in 72.949 CPU sec, 2.363 real sec
[M::process] read 699478 sequences (100000222 bp) in 2.699 CPU sec, 2.923 real sec...
sorting block 25, #reads = 1094433 mem = 536884050 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291210, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.95, 121.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698732 sequences (100000218 bp) in 2.265 CPU sec, 2.396 real sec...
[M::mem_process_seqs] Processed 699478 reads in 74.012 CPU sec, 2.554 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291330, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (251.94, 121.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698732 reads in 72.160 CPU sec, 2.352 real sec
sorting block 26, #reads = 1094250 mem = 536880831 peak = 1073442816
[M::process] read 699308 sequences (100000015 bp) in 2.652 CPU sec, 2.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291522, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.80, 119.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 271, 992)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2944)
[M::mem_pestat] mean and std.dev: (625.60, 832.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3955)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 698094 sequences (100000218 bp) in 1.999 CPU sec, 2.079 real sec...
[M::mem_process_seqs] Processed 699308 reads in 72.754 CPU sec, 2.358 real sec
sorting block 27, #reads = 1095164 mem = 536878262 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 290851, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (564, 1268, 2347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5913)
[M::mem_pestat] mean and std.dev: (1096.88, 713.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7696)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.56, 121.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697298 sequences (100000017 bp) in 2.163 CPU sec, 2.285 real sec...
[M::mem_process_seqs] Processed 698094 reads in 73.218 CPU sec, 2.362 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290462, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.65, 121.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 317, 984)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2392)
[M::mem_pestat] mean and std.dev: (626.90, 562.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3096)
[M::mem_pestat] skip orientation RR
[M::process] read 696952 sequences (100000153 bp) in 2.163 CPU sec, 2.282 real sec...
[M::mem_process_seqs] Processed 697298 reads in 72.406 CPU sec, 2.344 real sec
sorting block 28, #reads = 1093888 mem = 536877958 peak = 1073442816
[M::process] read 696922 sequences (100000017 bp) in 2.115 CPU sec, 2.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290648, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 440, 2076)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5774)
[M::mem_pestat] mean and std.dev: (819.08, 884.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7623)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.82, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697478 sequences (100000182 bp) in 1.490 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290380, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.01, 119.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (336, 441, 915)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2073)
[M::mem_pestat] mean and std.dev: (537.55, 300.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2652)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696952 reads in 128.691 CPU sec, 4.049 real sec
[M::mem_process_seqs] Processed 696922 reads in 83.363 CPU sec, 2.628 real sec
sorting block 29, #reads = 1092668 mem = 536879667 peak = 1073442816
[M::process] read 697154 sequences (100000039 bp) in 1.962 CPU sec, 2.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290369, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.86, 119.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696586 sequences (100000296 bp) in 2.290 CPU sec, 2.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290885, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.69, 119.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697478 reads in 132.640 CPU sec, 4.226 real sec
[M::mem_process_seqs] Processed 697154 reads in 86.387 CPU sec, 2.732 real sec
sorting block 30, #reads = 1092214 mem = 536882299 peak = 1073442816
[M::process] read 696246 sequences (100000165 bp) in 2.082 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290557, 6, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.57, 120.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 304, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (350.50, 188.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RR
sorting block 31, #reads = 1091342 mem = 536881371 peak = 1073442816
[M::process] read 697526 sequences (100000060 bp) in 2.219 CPU sec, 2.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290329, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.57, 120.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696586 reads in 131.104 CPU sec, 4.166 real sec
[M::mem_process_seqs] Processed 696246 reads in 85.137 CPU sec, 2.686 real sec
[M::process] read 704210 sequences (100000030 bp) in 1.899 CPU sec, 1.971 real sec...
sorting block 32, #reads = 1090831 mem = 536881354 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290509, 5, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 208, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (252.18, 126.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.08, 122.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701552 sequences (100000016 bp) in 2.231 CPU sec, 2.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292452, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (233.09, 109.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 283, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1413)
[M::mem_pestat] mean and std.dev: (362.38, 361.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1837)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697526 reads in 133.837 CPU sec, 4.264 real sec
[M::mem_process_seqs] Processed 704210 reads in 85.396 CPU sec, 2.692 real sec
[M::process] read 700526 sequences (100000244 bp) in 2.037 CPU sec, 2.116 real sec...
sorting block 33, #reads = 1089089 mem = 536880216 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291518, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.93, 115.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700082 sequences (100000094 bp) in 2.180 CPU sec, 2.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291863, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.97, 118.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701552 reads in 127.447 CPU sec, 4.053 real sec
sorting block 34, #reads = 1096389 mem = 536881683 peak = 1073442816
[M::process] read 699344 sequences (100000044 bp) in 2.007 CPU sec, 2.111 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291199, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.09, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700082 reads in 70.984 CPU sec, 2.244 real sec
[M::process] read 699024 sequences (100000165 bp) in 2.046 CPU sec, 2.107 real sec...
[M::mem_process_seqs] Processed 700526 reads in 188.802 CPU sec, 5.967 real sec
sorting block 35, #reads = 1095829 mem = 536877962 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290989, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (770, 1000, 1104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (102, 1772)
[M::mem_pestat] mean and std.dev: (866.18, 270.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2106)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.61, 120.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (667, 1133, 2203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5275)
[M::mem_pestat] mean and std.dev: (1154.00, 682.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6811)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291032, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 327, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (252.25, 102.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.48, 121.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698570 sequences (100000128 bp) in 2.581 CPU sec, 2.679 real sec...
[M::mem_process_seqs] Processed 699344 reads in 140.819 CPU sec, 4.464 real sec
[M::mem_process_seqs] Processed 699024 reads in 83.947 CPU sec, 2.654 real sec
sorting block 36, #reads = 1095559 mem = 536882978 peak = 1073442816
[M::process] read 698564 sequences (100000201 bp) in 2.281 CPU sec, 2.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290906, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.21, 121.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290909, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.66, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698614 sequences (100000133 bp) in 2.043 CPU sec, 2.128 real sec...
[M::mem_process_seqs] Processed 698570 reads in 127.773 CPU sec, 4.077 real sec
sorting block 37, #reads = 1094339 mem = 536879127 peak = 1073442816
[M::mem_process_seqs] Processed 698564 reads in 82.719 CPU sec, 2.619 real sec
[M::process] read 697030 sequences (100000098 bp) in 2.279 CPU sec, 2.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291298, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.45, 121.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1094099 mem = 536879907 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290386, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 387, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2217)
[M::mem_pestat] mean and std.dev: (405.30, 297.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2904)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.70, 121.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696236 sequences (100000246 bp) in 2.056 CPU sec, 2.128 real sec...
[M::mem_process_seqs] Processed 698614 reads in 127.545 CPU sec, 4.067 real sec
[M::mem_process_seqs] Processed 697030 reads in 82.143 CPU sec, 2.600 real sec
sorting block 39, #reads = 1092878 mem = 536879242 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290224, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.52, 120.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696532 sequences (100000132 bp) in 2.375 CPU sec, 2.526 real sec...
[M::mem_process_seqs] Processed 696236 reads in 71.358 CPU sec, 2.352 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290170, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 312, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (303.11, 126.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.32, 118.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696532 reads in 71.803 CPU sec, 2.363 real sec
[M::process] read 696522 sequences (100000014 bp) in 2.583 CPU sec, 2.794 real sec...
sorting block 40, #reads = 1091966 mem = 536879588 peak = 1073442816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290110, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 253, 1600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4418)
[M::mem_pestat] mean and std.dev: (686.71, 635.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5827)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.71, 119.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (308, 486, 4626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13262)
[M::mem_pestat] mean and std.dev: (1564.77, 1966.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17580)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696522 reads in 72.441 CPU sec, 2.366 real sec
[M::process] read 696676 sequences (100000004 bp) in 2.430 CPU sec, 2.608 real sec...
sorting block 41, #reads = 1091466 mem = 536880751 peak = 1125335040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290705, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.27, 118.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696676 reads in 71.586 CPU sec, 2.330 real sec
[M::process] read 696518 sequences (100000253 bp) in 2.733 CPU sec, 2.964 real sec...
sorting block 42, #reads = 1091281 mem = 536878784 peak = 1125335040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290491, 12, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.28, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (64, 106, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078)
[M::mem_pestat] mean and std.dev: (268.83, 291.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1433)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 200, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (254.18, 117.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696518 reads in 70.259 CPU sec, 2.266 real sec
[M::process] read 696478 sequences (100000233 bp) in 2.257 CPU sec, 2.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290255, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.53, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696478 reads in 71.596 CPU sec, 2.327 real sec
[M::process] read 696284 sequences (100000154 bp) in 2.381 CPU sec, 2.512 real sec...
sorting block 43, #reads = 1091343 mem = 536880170 peak = 1125335040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290034, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (255.92, 122.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700672 sequences (100000260 bp) in 2.131 CPU sec, 2.230 real sec...
[M::mem_process_seqs] Processed 696284 reads in 72.282 CPU sec, 2.340 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291626, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 341, 651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507)
[M::mem_pestat] mean and std.dev: (480.33, 397.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2071)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (243.50, 116.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 705570 sequences (100000185 bp) in 2.191 CPU sec, 2.295 real sec...
[M::mem_process_seqs] Processed 700672 reads in 76.183 CPU sec, 2.453 real sec
sorting block 44, #reads = 1090168 mem = 536879302 peak = 1125335040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293313, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.95, 108.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705474 sequences (100000214 bp) in 2.465 CPU sec, 2.625 real sec...
[M::mem_process_seqs] Processed 705570 reads in 78.051 CPU sec, 2.516 real sec
sorting block 45, #reads = 1092481 mem = 536879392 peak = 1125335040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293341, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (233.09, 111.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704380 sequences (100000269 bp) in 2.362 CPU sec, 2.506 real sec...
[M::mem_process_seqs] Processed 705474 reads in 76.123 CPU sec, 2.453 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292951, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.94, 113.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702834 sequences (100000166 bp) in 2.143 CPU sec, 2.236 real sec...
[M::mem_process_seqs] Processed 704380 reads in 73.321 CPU sec, 2.367 real sec
sorting block 46, #reads = 1094324 mem = 536884197 peak = 1125335040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292650, 7, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (299, 299, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (267, 347)
[M::mem_pestat] mean and std.dev: (298.00, 13.33)
[M::mem_pestat] low and high boundaries for proper pairs: (245, 363)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (241.20, 116.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 302, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (304.67, 99.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 702122 sequences (100000202 bp) in 2.185 CPU sec, 2.292 real sec...
[M::mem_process_seqs] Processed 702834 reads in 73.232 CPU sec, 2.361 real sec
sorting block 47, #reads = 1095659 mem = 536877231 peak = 1125335040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292433, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.11, 116.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702000 sequences (100000114 bp) in 2.148 CPU sec, 2.235 real sec...
[M::mem_process_seqs] Processed 702122 reads in 72.916 CPU sec, 2.346 real sec
[M::process] read 701212 sequences (100000040 bp) in 2.126 CPU sec, 2.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291972, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (435, 1659, 2478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6564)
[M::mem_pestat] mean and std.dev: (1354.75, 1014.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.44, 118.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701484 sequences (100000020 bp) in 1.500 CPU sec, 1.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292166, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (321, 346, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (271, 396)
[M::mem_pestat] mean and std.dev: (341.50, 9.18)
[M::mem_pestat] low and high boundaries for proper pairs: (246, 421)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.61, 118.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702000 reads in 131.076 CPU sec, 4.117 real sec
[M::mem_process_seqs] Processed 701212 reads in 82.831 CPU sec, 2.601 real sec
sorting block 48, #reads = 1094082 mem = 536881092 peak = 1125335040
[M::process] read 701350 sequences (100000127 bp) in 1.953 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291962, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.01, 119.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 264, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (56, 481)
[M::mem_pestat] mean and std.dev: (254.27, 92.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 623)
[M::mem_pestat] skip orientation RR
sorting block 49, #reads = 1094406 mem = 536876530 peak = 1125335040
[M::process] read 700864 sequences (100000129 bp) in 2.659 CPU sec, 2.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291847, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.20, 119.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 247, 676)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1580)
[M::mem_pestat] mean and std.dev: (297.64, 147.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2032)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701484 reads in 131.241 CPU sec, 4.180 real sec
[M::mem_process_seqs] Processed 701350 reads in 85.052 CPU sec, 2.686 real sec
sorting block 50, #reads = 1093635 mem = 536882242 peak = 1125335040
[M::process] read 700430 sequences (100000046 bp) in 1.998 CPU sec, 2.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291808, 11, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (246.05, 119.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 303, 1267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3535)
[M::mem_pestat] mean and std.dev: (742.27, 940.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4669)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 699296 sequences (100000164 bp) in 1.920 CPU sec, 1.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291625, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 261, 2478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7066)
[M::mem_pestat] mean and std.dev: (1134.58, 1135.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9360)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.08, 119.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700864 reads in 127.109 CPU sec, 4.030 real sec
[M::mem_process_seqs] Processed 700430 reads in 81.953 CPU sec, 2.582 real sec
[M::process] read 698214 sequences (100000036 bp) in 2.079 CPU sec, 2.186 real sec...
sorting block 51, #reads = 1092576 mem = 536875981 peak = 1125335040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291249, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.01, 120.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698004 sequences (100000244 bp) in 2.290 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290785, 10, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 418, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3113)
[M::mem_pestat] mean and std.dev: (598.20, 591.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4109)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.00, 119.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 421, 689)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815)
[M::mem_pestat] mean and std.dev: (438.90, 374.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2378)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 699296 reads in 132.632 CPU sec, 4.242 real sec
[M::mem_process_seqs] Processed 698214 reads in 86.294 CPU sec, 2.725 real sec
sorting block 52, #reads = 1092663 mem = 536879390 peak = 1125335040
[M::process] read 697258 sequences (100000293 bp) in 2.144 CPU sec, 2.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290776, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.12, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697518 sequences (100000019 bp) in 2.107 CPU sec, 2.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290517, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 225, 951)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2547)
[M::mem_pestat] mean and std.dev: (507.87, 491.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3345)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.19, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 244, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (114, 334)
[M::mem_pestat] mean and std.dev: (212.69, 41.70)
[M::mem_pestat] low and high boundaries for proper pairs: (46, 380)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698004 reads in 132.948 CPU sec, 4.219 real sec
sorting block 53, #reads = 1090918 mem = 536878675 peak = 1125335040
[M::mem_process_seqs] Processed 697258 reads in 86.415 CPU sec, 2.716 real sec
[M::process] read 706288 sequences (100000052 bp) in 2.150 CPU sec, 2.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290895, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.53, 121.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (335, 480, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 770)
[M::mem_pestat] mean and std.dev: (391.23, 125.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RR
sorting block 54, #reads = 1089920 mem = 536879954 peak = 1127624704
[M::process] read 709870 sequences (100000185 bp) in 2.296 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293342, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (228.97, 107.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 736)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697518 reads in 139.889 CPU sec, 4.440 real sec
[M::mem_process_seqs] Processed 706288 reads in 94.982 CPU sec, 2.992 real sec
[M::process] read 708846 sequences (100000035 bp) in 2.002 CPU sec, 2.049 real sec...
sorting block 55, #reads = 1089788 mem = 536882603 peak = 1127624704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 294118, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 199, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (221.11, 102.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 700)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706740 sequences (100000011 bp) in 2.311 CPU sec, 2.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 294335, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.09, 105.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 709870 reads in 145.779 CPU sec, 4.628 real sec
[M::process] read 705206 sequences (100000071 bp) in 1.644 CPU sec, 1.663 real sec...
[M::mem_process_seqs] Processed 708846 reads in 94.303 CPU sec, 2.970 real sec
sorting block 56, #reads = 1087458 mem = 536878113 peak = 1127624704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293917, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.62, 108.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703670 sequences (100000249 bp) in 2.319 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293834, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.55, 111.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706740 reads in 136.000 CPU sec, 4.322 real sec
sorting block 57, #reads = 1089333 mem = 536878488 peak = 1127624704
[M::mem_process_seqs] Processed 705206 reads in 87.531 CPU sec, 2.762 real sec
[M::process] read 703240 sequences (100000231 bp) in 2.096 CPU sec, 2.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292609, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (237.12, 113.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 261, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1587)
[M::mem_pestat] mean and std.dev: (336.92, 222.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2067)
[M::mem_pestat] skip orientation RR
sorting block 58, #reads = 1091150 mem = 536880890 peak = 1134260224
[M::process] read 702684 sequences (100000216 bp) in 2.222 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292955, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.64, 114.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703670 reads in 131.025 CPU sec, 4.181 real sec
[M::mem_process_seqs] Processed 703240 reads in 85.347 CPU sec, 2.700 real sec
[M::process] read 702098 sequences (100000264 bp) in 1.857 CPU sec, 1.959 real sec...
sorting block 59, #reads = 1090570 mem = 536882067 peak = 1134260224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292374, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.67, 114.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701282 sequences (100000152 bp) in 2.006 CPU sec, 2.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292241, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.77, 115.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702684 reads in 127.909 CPU sec, 4.083 real sec
[M::mem_process_seqs] Processed 702098 reads in 84.108 CPU sec, 2.662 real sec
[M::process] read 700480 sequences (100000188 bp) in 1.599 CPU sec, 1.631 real sec...
sorting block 60, #reads = 1091102 mem = 536879415 peak = 1134260224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292119, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.65, 116.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 355, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (311.40, 104.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RR
[M::process] read 699872 sequences (100000209 bp) in 2.268 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291640, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (246.01, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701282 reads in 124.913 CPU sec, 3.985 real sec
[M::mem_process_seqs] Processed 700480 reads in 81.788 CPU sec, 2.587 real sec
sorting block 61, #reads = 1092032 mem = 536878187 peak = 1134260224
[M::process] read 698680 sequences (100000076 bp) in 1.697 CPU sec, 1.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291408, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 172, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593)
[M::mem_pestat] mean and std.dev: (176.55, 85.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.92, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 62, #reads = 1092048 mem = 536879141 peak = 1134260224
[M::process] read 698234 sequences (100000295 bp) in 2.181 CPU sec, 2.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290735, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 285, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (243.82, 94.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.24, 120.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699872 reads in 125.360 CPU sec, 3.998 real sec
[M::mem_process_seqs] Processed 698680 reads in 82.600 CPU sec, 2.612 real sec
[M::process] read 698108 sequences (100000185 bp) in 1.765 CPU sec, 1.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290521, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (295, 295, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (203, 433)
[M::mem_pestat] mean and std.dev: (302.43, 43.68)
[M::mem_pestat] low and high boundaries for proper pairs: (128, 479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.05, 121.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 63, #reads = 1091531 mem = 536879301 peak = 1134260224
[M::process] read 699416 sequences (100000107 bp) in 2.216 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290494, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.56, 121.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698234 reads in 125.136 CPU sec, 3.992 real sec
[M::mem_process_seqs] Processed 698108 reads in 82.998 CPU sec, 2.632 real sec
[M::process] read 705986 sequences (100000031 bp) in 2.198 CPU sec, 2.355 real sec...
sorting block 64, #reads = 1089794 mem = 536878286 peak = 1134260224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290443, 7, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.88, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 280, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (324.76, 165.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1511)
[M::mem_pestat] skip orientation RR
[M::process] read 703740 sequences (100000002 bp) in 2.012 CPU sec, 2.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293357, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 289, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (354.00, 189.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.32, 108.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699416 reads in 137.178 CPU sec, 4.376 real sec
[M::mem_process_seqs] Processed 705986 reads in 86.831 CPU sec, 2.746 real sec
[M::process] read 702966 sequences (100000043 bp) in 1.809 CPU sec, 1.891 real sec...
sorting block 65, #reads = 1087783 mem = 536878432 peak = 1146703872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292802, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (236.46, 112.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 268, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (292.08, 113.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RR
[M::process] read 702038 sequences (100000045 bp) in 2.278 CPU sec, 2.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292859, 11, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (238.43, 114.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 170, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318)
[M::mem_pestat] mean and std.dev: (315.91, 258.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1706)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 703740 reads in 122.211 CPU sec, 3.915 real sec
sorting block 66, #reads = 1089235 mem = 536877774 peak = 1146703872
[M::mem_process_seqs] Processed 702966 reads in 80.291 CPU sec, 2.556 real sec
[M::process] read 701754 sequences (100000293 bp) in 2.446 CPU sec, 2.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292766, 8, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 241, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (199.44, 85.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.58, 115.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 67, #reads = 1094013 mem = 536880332 peak = 1146703872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292085, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (242.04, 115.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702038 reads in 121.416 CPU sec, 3.901 real sec
[M::process] read 700614 sequences (100000265 bp) in 2.383 CPU sec, 2.535 real sec...
[M::mem_process_seqs] Processed 701754 reads in 80.265 CPU sec, 2.554 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291557, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.64, 116.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1095086 mem = 536878694 peak = 1146703872
[M::mem_process_seqs] Processed 700614 reads in 67.480 CPU sec, 2.370 real sec
[M::process] read 700524 sequences (100000223 bp) in 2.943 CPU sec, 3.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292249, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.62, 118.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700524 reads in 66.661 CPU sec, 2.200 real sec
sorting block 69, #reads = 1094000 mem = 536886922 peak = 1146703872
[M::process] read 700986 sequences (100000226 bp) in 2.544 CPU sec, 2.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292150, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 277, 1569)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4465)
[M::mem_pestat] mean and std.dev: (918.60, 987.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5913)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.31, 115.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700986 reads in 67.612 CPU sec, 2.190 real sec
[M::process] read 701280 sequences (100000238 bp) in 2.066 CPU sec, 2.199 real sec...
sorting block 70, #reads = 1093276 mem = 536879440 peak = 1146703872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291922, 9, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 204, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (79, 329)
[M::mem_pestat] mean and std.dev: (182.50, 43.91)
[M::mem_pestat] low and high boundaries for proper pairs: (7, 379)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.90, 116.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701280 reads in 68.591 CPU sec, 2.223 real sec
[M::process] read 700030 sequences (100000029 bp) in 2.128 CPU sec, 2.285 real sec...
sorting block 71, #reads = 1093732 mem = 536878871 peak = 1146703872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291692, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.36, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700030 reads in 68.088 CPU sec, 2.206 real sec
[M::process] read 698912 sequences (100000024 bp) in 2.152 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291180, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.33, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699108 sequences (100000086 bp) in 2.015 CPU sec, 2.132 real sec...
[M::mem_process_seqs] Processed 698912 reads in 68.309 CPU sec, 2.216 real sec
sorting block 72, #reads = 1092824 mem = 536880864 peak = 1146703872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291539, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (274, 359, 1550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4102)
[M::mem_pestat] mean and std.dev: (858.36, 880.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5378)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.16, 120.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699108 reads in 69.096 CPU sec, 2.242 real sec
[M::process] read 699292 sequences (100000139 bp) in 2.785 CPU sec, 2.970 real sec...
sorting block 73, #reads = 1092162 mem = 536880906 peak = 1146703872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290992, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (247.02, 118.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 210, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1649)
[M::mem_pestat] mean and std.dev: (295.75, 206.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2152)
[M::mem_pestat] skip orientation RR
[M::process] read 697898 sequences (100000011 bp) in 1.845 CPU sec, 2.015 real sec...
[M::process] read 698230 sequences (100000008 bp) in 1.280 CPU sec, 1.274 real sec...
[M::process] read 698180 sequences (100000253 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290622, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.68, 119.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698230 reads in 66.809 CPU sec, 2.250 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290567, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.09, 120.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 257, 801)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2057)
[M::mem_pestat] mean and std.dev: (366.00, 276.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2685)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697898 reads in 142.659 CPU sec, 4.998 real sec
sorting block 74, #reads = 1090058 mem = 536878936 peak = 1146703872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289827, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.08, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699292 reads in 270.779 CPU sec, 9.139 real sec
[M::mem_process_seqs] Processed 698180 reads in 86.384 CPU sec, 2.792 real sec
[M::process] read 700740 sequences (100000255 bp) in 2.944 CPU sec, 3.126 real sec...
sorting block 75, #reads = 1090069 mem = 536879381 peak = 1146703872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291057, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.99, 117.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 214, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (215.78, 113.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700740 reads in 83.551 CPU sec, 2.746 real sec
[M::process] read 711718 sequences (100000164 bp) in 2.571 CPU sec, 2.793 real sec...
sorting block 76, #reads = 1085634 mem = 536882641 peak = 1146703872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 294965, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 196, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (216.87, 100.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706908 sequences (100000238 bp) in 2.060 CPU sec, 2.189 real sec...
[M::mem_process_seqs] Processed 711718 reads in 83.230 CPU sec, 2.695 real sec
[M::process] read 705578 sequences (100000037 bp) in 2.058 CPU sec, 2.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293741, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.38, 109.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703812 sequences (100000104 bp) in 1.610 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293233, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 189, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (198.22, 82.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (233.23, 111.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 706908 reads in 137.550 CPU sec, 4.324 real sec
sorting block 77, #reads = 1086498 mem = 536879382 peak = 1146703872
[M::mem_process_seqs] Processed 705578 reads in 89.770 CPU sec, 2.823 real sec
[M::process] read 702692 sequences (100000133 bp) in 1.737 CPU sec, 1.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293119, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.29, 114.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1088492 mem = 536878855 peak = 1155162112
[M::process] read 701488 sequences (100000093 bp) in 2.597 CPU sec, 2.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292237, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (242.05, 117.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703812 reads in 134.001 CPU sec, 4.284 real sec
[M::mem_process_seqs] Processed 702692 reads in 88.542 CPU sec, 2.803 real sec
[M::process] read 700784 sequences (100000097 bp) in 2.036 CPU sec, 2.129 real sec...
sorting block 79, #reads = 1088747 mem = 536882784 peak = 1155162112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291684, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.80, 119.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699562 sequences (100000236 bp) in 2.109 CPU sec, 2.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291779, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.52, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 686, 1012)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2696)
[M::mem_pestat] mean and std.dev: (519.44, 445.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3538)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701488 reads in 132.875 CPU sec, 4.223 real sec
[M::mem_process_seqs] Processed 700784 reads in 86.313 CPU sec, 2.726 real sec
[M::process] read 699330 sequences (100000173 bp) in 2.105 CPU sec, 2.192 real sec...
sorting block 80, #reads = 1088736 mem = 536878929 peak = 1155162112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291445, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.50, 121.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 324, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (292.55, 138.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1416)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291678, 10, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.65, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 475, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1506)
[M::mem_pestat] mean and std.dev: (421.20, 251.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1935)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 698162 sequences (100000267 bp) in 2.384 CPU sec, 2.569 real sec...
[M::mem_process_seqs] Processed 699562 reads in 128.320 CPU sec, 4.109 real sec
[M::mem_process_seqs] Processed 699330 reads in 84.456 CPU sec, 2.676 real sec
sorting block 81, #reads = 1089440 mem = 536881361 peak = 1155162112
[M::process] read 698132 sequences (100000266 bp) in 2.332 CPU sec, 2.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290565, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.88, 119.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 287, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (333.38, 172.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1352)
[M::mem_pestat] skip orientation RR
sorting block 82, #reads = 1090299 mem = 536879420 peak = 1155162112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290817, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 387, 1268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3388)
[M::mem_pestat] mean and std.dev: (607.30, 473.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4448)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.53, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698010 sequences (100000236 bp) in 2.145 CPU sec, 2.220 real sec...
[M::mem_process_seqs] Processed 698162 reads in 126.511 CPU sec, 4.048 real sec
[M::mem_process_seqs] Processed 698132 reads in 82.580 CPU sec, 2.619 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290887, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 242, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 420)
[M::mem_pestat] mean and std.dev: (240.45, 44.48)
[M::mem_pestat] low and high boundaries for proper pairs: (12, 488)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.13, 119.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697240 sequences (100000203 bp) in 2.430 CPU sec, 2.630 real sec...
[M::mem_process_seqs] Processed 698010 reads in 69.825 CPU sec, 2.315 real sec
sorting block 83, #reads = 1089516 mem = 536877404 peak = 1155162112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290422, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.87, 120.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 208, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (253.92, 113.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RR
[M::process] read 697524 sequences (100000036 bp) in 2.172 CPU sec, 2.344 real sec...
[M::mem_process_seqs] Processed 697240 reads in 70.564 CPU sec, 2.324 real sec
sorting block 84, #reads = 1090070 mem = 536878971 peak = 1155162112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290280, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.48, 120.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697524 reads in 70.364 CPU sec, 2.311 real sec
[M::process] read 697132 sequences (100000024 bp) in 2.484 CPU sec, 2.683 real sec...
sorting block 85, #reads = 1089403 mem = 536880713 peak = 1155162112
[M::process] read 485214 sequences (69537185 bp) in 1.508 CPU sec, 1.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290653, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.66, 121.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 202221, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (251.80, 121.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697132 reads in 106.028 CPU sec, 3.413 real sec
[M::mem_process_seqs] Processed 485214 reads in 60.253 CPU sec, 1.960 real sec
sorting block 86, #reads = 1090167 mem = 536879498 peak = 1155162112
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 94986792
overall: 70727249920 mem 10530.760 user 345.684 sys 1025.820 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1025.857 sec; CPU: 10876.447 sec
sorting block 87, #reads = 444790 mem = 219325256 peak = 1155162112
uniq header count 1
merging 88 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5273287987
output reads: 95471801
bam_mem_sort: 88 calls 32.137 user 0.396 sys 32.694 real
bam_write: 88 calls 91.239 user 2.052 sys 93.679 real
execute: 1 calls 21.221 user 0.684 sys 1054.804 real
merge_files: 1 calls 21.197 user 0.661 sys 28.231 real
parse_chunk: 1399 calls 51.908 user 14.687 sys 67.448 real
read_chunk: 3779 calls 34.530 user 25.323 sys 439.086 real
sort_block: 1 calls 24.124 user 24.990 sys 424.498 real
write_chunk: 2378 calls 20.801 user 0.428 sys 27.358 real
overall: 3000143872 mem 1094.813 user 48.820 sys 1054.827 real
ls: cannot access '*.cram': No such file or directory