This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_bR4-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1816.766 CPU sec, 70.429 real sec
[M::process] read 677668 sequences (100000222 bp) in 1.377 CPU sec, 1.382 real sec...
[M::process] read 677778 sequences (100000156 bp) in 2.254 CPU sec, 2.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282104, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 326, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (344.16, 155.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677740 sequences (100000298 bp) in 2.086 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282277, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (346.11, 156.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677668 reads in 199.151 CPU sec, 6.544 real sec
[M::process] read 677618 sequences (100000138 bp) in 2.047 CPU sec, 2.095 real sec...
[M::mem_process_seqs] Processed 677778 reads in 118.684 CPU sec, 3.824 real sec
sorting block 0, #reads = 1081892 mem = 536878071 peak = 562786304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282521, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 328, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (344.35, 154.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677384 sequences (100000141 bp) in 2.537 CPU sec, 2.703 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281257, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 328, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (343.93, 154.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677172 sequences (100000080 bp) in 2.179 CPU sec, 2.218 real sec...
[M::mem_process_seqs] Processed 677740 reads in 184.177 CPU sec, 5.931 real sec
[M::mem_process_seqs] Processed 677618 reads in 121.330 CPU sec, 3.899 real sec
sorting block 1, #reads = 1081211 mem = 536879456 peak = 691486720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282150, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (345.10, 154.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677006 sequences (100000183 bp) in 2.673 CPU sec, 3.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282021, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (348.50, 156.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677384 reads in 184.986 CPU sec, 6.304 real sec
[M::process] read 677120 sequences (100000157 bp) in 2.463 CPU sec, 2.628 real sec...
[M::mem_process_seqs] Processed 677172 reads in 121.493 CPU sec, 4.355 real sec
sorting block 2, #reads = 1081689 mem = 536878842 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281920, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (349.56, 156.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281956, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (351.61, 157.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676712 sequences (100000264 bp) in 3.297 CPU sec, 3.983 real sec...
[M::mem_process_seqs] Processed 677006 reads in 181.430 CPU sec, 6.368 real sec
[M::mem_process_seqs] Processed 677120 reads in 122.218 CPU sec, 4.437 real sec
sorting block 3, #reads = 1081461 mem = 536880020 peak = 805535744
[M::process] read 677084 sequences (100000073 bp) in 2.847 CPU sec, 3.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281935, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (354.90, 158.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676712 reads in 103.828 CPU sec, 4.041 real sec
[M::process] read 677598 sequences (100000066 bp) in 3.047 CPU sec, 3.502 real sec...
sorting block 4, #reads = 1080526 mem = 536883848 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281916, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.44, 158.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282130, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (348.36, 158.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676074 sequences (100000075 bp) in 3.026 CPU sec, 3.537 real sec...
[M::mem_process_seqs] Processed 677084 reads in 188.823 CPU sec, 6.679 real sec
[M::mem_process_seqs] Processed 677598 reads in 126.454 CPU sec, 4.779 real sec
sorting block 5, #reads = 1079708 mem = 536878064 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 280648, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (300, 1491, 1491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3873)
[M::mem_pestat] mean and std.dev: (883.21, 610.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5064)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (360.35, 160.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675772 sequences (100000177 bp) in 3.515 CPU sec, 4.156 real sec...
[M::mem_process_seqs] Processed 676074 reads in 104.254 CPU sec, 4.114 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280880, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (361.78, 160.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675540 sequences (100000111 bp) in 3.087 CPU sec, 3.713 real sec...
[M::mem_process_seqs] Processed 675772 reads in 105.153 CPU sec, 4.237 real sec
sorting block 6, #reads = 1080046 mem = 536878824 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280583, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (364.27, 161.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675158 sequences (100000013 bp) in 3.193 CPU sec, 3.561 real sec...
[M::mem_process_seqs] Processed 675540 reads in 105.568 CPU sec, 4.247 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281140, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (366.35, 161.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675028 sequences (100000287 bp) in 3.201 CPU sec, 3.686 real sec...
sorting block 7, #reads = 1080046 mem = 536879214 peak = 805535744
[M::mem_process_seqs] Processed 675158 reads in 104.282 CPU sec, 4.256 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280994, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (366.97, 161.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675012 sequences (100000196 bp) in 3.002 CPU sec, 3.392 real sec...
[M::mem_process_seqs] Processed 675028 reads in 102.813 CPU sec, 4.335 real sec
sorting block 8, #reads = 1079642 mem = 536879223 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281027, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (365.34, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675098 sequences (100000076 bp) in 3.297 CPU sec, 3.692 real sec...
[M::mem_process_seqs] Processed 675012 reads in 104.020 CPU sec, 4.279 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281221, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (364.85, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674988 sequences (100000117 bp) in 3.065 CPU sec, 3.417 real sec...
[M::mem_process_seqs] Processed 675098 reads in 106.641 CPU sec, 4.210 real sec
sorting block 9, #reads = 1079490 mem = 536880617 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281442, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (366.68, 160.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675402 sequences (100000004 bp) in 3.033 CPU sec, 3.414 real sec...
[M::mem_process_seqs] Processed 674988 reads in 104.704 CPU sec, 4.258 real sec
sorting block 10, #reads = 1080405 mem = 536878061 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280883, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (361.55, 159.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675504 sequences (100000159 bp) in 3.050 CPU sec, 3.404 real sec...
[M::mem_process_seqs] Processed 675402 reads in 105.855 CPU sec, 4.405 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281532, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (358.27, 158.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674430 sequences (100000216 bp) in 3.086 CPU sec, 3.507 real sec...
[M::mem_process_seqs] Processed 675504 reads in 108.836 CPU sec, 4.345 real sec
sorting block 11, #reads = 1080901 mem = 536878696 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280408, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (363.82, 158.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674246 sequences (100000271 bp) in 3.229 CPU sec, 3.647 real sec...
[M::mem_process_seqs] Processed 674430 reads in 110.065 CPU sec, 4.399 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280325, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (367.00, 159.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 12, #reads = 1079334 mem = 536878295 peak = 805535744
[M::process] read 674218 sequences (100000178 bp) in 3.263 CPU sec, 3.625 real sec...
[M::mem_process_seqs] Processed 674246 reads in 105.527 CPU sec, 4.219 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280661, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (368.38, 159.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674360 sequences (100000060 bp) in 3.353 CPU sec, 3.915 real sec...
[M::mem_process_seqs] Processed 674218 reads in 104.953 CPU sec, 4.289 real sec
sorting block 13, #reads = 1079407 mem = 536884225 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281139, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (368.79, 160.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673780 sequences (100000081 bp) in 3.410 CPU sec, 3.865 real sec...
[M::mem_process_seqs] Processed 674360 reads in 105.416 CPU sec, 4.102 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280888, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 356, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (369.55, 159.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673924 sequences (100000000 bp) in 3.275 CPU sec, 3.621 real sec...
[M::mem_process_seqs] Processed 673780 reads in 104.238 CPU sec, 4.044 real sec
sorting block 14, #reads = 1079675 mem = 536877867 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280415, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (366.77, 157.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673816 sequences (100000096 bp) in 3.359 CPU sec, 3.717 real sec...
[M::mem_process_seqs] Processed 673924 reads in 106.161 CPU sec, 4.003 real sec
sorting block 15, #reads = 1079818 mem = 536879349 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280982, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (367.11, 158.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674016 sequences (100000100 bp) in 3.332 CPU sec, 3.602 real sec...
[M::mem_process_seqs] Processed 673816 reads in 105.995 CPU sec, 3.833 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280756, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (365.32, 158.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674104 sequences (100000298 bp) in 3.430 CPU sec, 3.748 real sec...
[M::mem_process_seqs] Processed 674016 reads in 109.522 CPU sec, 3.797 real sec
sorting block 16, #reads = 1080120 mem = 536880134 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280515, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (366.03, 159.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674072 sequences (100000204 bp) in 3.407 CPU sec, 3.679 real sec...
[M::mem_process_seqs] Processed 674104 reads in 112.545 CPU sec, 3.860 real sec
sorting block 17, #reads = 1079923 mem = 536878814 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280545, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (365.32, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674208 sequences (100000185 bp) in 3.328 CPU sec, 3.654 real sec...
[M::mem_process_seqs] Processed 674072 reads in 111.885 CPU sec, 3.784 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280146, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 348, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (363.59, 157.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674274 sequences (100000224 bp) in 3.333 CPU sec, 3.606 real sec...
[M::mem_process_seqs] Processed 674208 reads in 111.002 CPU sec, 3.760 real sec
sorting block 18, #reads = 1079206 mem = 536879097 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280264, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (362.26, 156.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674274 reads in 108.459 CPU sec, 3.719 real sec
[M::process] read 674270 sequences (100000240 bp) in 3.448 CPU sec, 3.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280989, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (363.04, 157.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1079050 mem = 536879201 peak = 805535744
[M::mem_process_seqs] Processed 674270 reads in 104.906 CPU sec, 3.513 real sec
[M::process] read 673920 sequences (100000134 bp) in 3.316 CPU sec, 3.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280471, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 352, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (366.81, 158.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673758 sequences (100000258 bp) in 3.142 CPU sec, 3.366 real sec...
[M::mem_process_seqs] Processed 673920 reads in 102.505 CPU sec, 3.405 real sec
sorting block 20, #reads = 1079415 mem = 536880436 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280654, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 354, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (368.49, 159.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673758 reads in 98.516 CPU sec, 3.275 real sec
[M::process] read 673830 sequences (100000025 bp) in 3.590 CPU sec, 3.887 real sec...
sorting block 21, #reads = 1078849 mem = 536879096 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280733, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (367.58, 159.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673830 reads in 95.877 CPU sec, 3.171 real sec
[M::process] read 673644 sequences (100000185 bp) in 3.792 CPU sec, 4.218 real sec...
sorting block 22, #reads = 1079582 mem = 536878928 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280447, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 353, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (367.84, 158.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673644 reads in 99.110 CPU sec, 3.213 real sec
[M::process] read 673386 sequences (100000076 bp) in 3.449 CPU sec, 3.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280259, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 357, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (371.92, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673546 sequences (100000051 bp) in 2.784 CPU sec, 3.057 real sec...
[M::mem_process_seqs] Processed 673386 reads in 95.925 CPU sec, 3.087 real sec
sorting block 23, #reads = 1079474 mem = 536878047 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281194, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 357, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (371.57, 160.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673546 reads in 94.241 CPU sec, 3.041 real sec
[M::process] read 676930 sequences (100000239 bp) in 3.174 CPU sec, 3.402 real sec...
sorting block 24, #reads = 1078916 mem = 536877847 peak = 805535744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281869, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (351.29, 158.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677632 sequences (100000261 bp) in 2.932 CPU sec, 3.156 real sec...
[M::mem_process_seqs] Processed 676930 reads in 99.744 CPU sec, 3.223 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281827, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (348.87, 158.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (746, 1361, 1479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2945)
[M::mem_pestat] mean and std.dev: (1339.00, 807.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4570)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677632 reads in 94.235 CPU sec, 3.041 real sec
sorting block 25, #reads = 1080852 mem = 536878613 peak = 805535744
[M::process] read 677462 sequences (100000260 bp) in 3.707 CPU sec, 3.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281568, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (348.59, 157.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677138 sequences (100000271 bp) in 2.892 CPU sec, 3.012 real sec...
[M::mem_process_seqs] Processed 677462 reads in 95.480 CPU sec, 3.056 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281597, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (352.12, 158.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 19, 40)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 82)
[M::mem_pestat] mean and std.dev: (24.25, 9.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 103)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677138 reads in 91.706 CPU sec, 2.948 real sec
sorting block 26, #reads = 1082636 mem = 536879051 peak = 805535744
[M::process] read 677090 sequences (100000118 bp) in 3.724 CPU sec, 4.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281228, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (351.72, 157.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677172 sequences (100000137 bp) in 2.649 CPU sec, 2.825 real sec...
[M::mem_process_seqs] Processed 677090 reads in 95.288 CPU sec, 3.050 real sec
sorting block 27, #reads = 1081018 mem = 536887359 peak = 811405312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282125, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (350.08, 156.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677172 reads in 89.706 CPU sec, 2.879 real sec
[M::process] read 676990 sequences (100000239 bp) in 3.690 CPU sec, 3.926 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281263, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 338, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (353.48, 158.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676764 sequences (100000243 bp) in 2.554 CPU sec, 2.761 real sec...
[M::mem_process_seqs] Processed 676990 reads in 91.207 CPU sec, 2.936 real sec
sorting block 28, #reads = 1082359 mem = 536880423 peak = 811405312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281441, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (354.64, 158.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676764 reads in 89.551 CPU sec, 2.876 real sec
[M::process] read 676512 sequences (100000170 bp) in 3.186 CPU sec, 3.394 real sec...
sorting block 29, #reads = 1081072 mem = 536876766 peak = 811405312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281039, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (355.84, 158.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676512 reads in 91.588 CPU sec, 2.950 real sec
[M::process] read 676450 sequences (100000123 bp) in 3.112 CPU sec, 3.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281032, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 342, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (357.01, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676450 reads in 91.255 CPU sec, 2.933 real sec
[M::process] read 676190 sequences (100000055 bp) in 3.271 CPU sec, 3.500 real sec...
sorting block 30, #reads = 1081063 mem = 536878913 peak = 811405312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281415, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (359.70, 160.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676190 reads in 93.603 CPU sec, 3.001 real sec
[M::process] read 676108 sequences (100000289 bp) in 2.852 CPU sec, 3.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281439, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (364.02, 162.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1080790 mem = 536880491 peak = 839004160
[M::mem_process_seqs] Processed 676108 reads in 89.855 CPU sec, 2.904 real sec
[M::process] read 675702 sequences (100000299 bp) in 3.514 CPU sec, 3.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281153, 3, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (392, 895, 1407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3437)
[M::mem_pestat] mean and std.dev: (772.10, 468.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4452)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (365.72, 162.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675702 reads in 88.359 CPU sec, 2.834 real sec
[M::process] read 675502 sequences (100000181 bp) in 2.870 CPU sec, 2.979 real sec...
sorting block 32, #reads = 1080294 mem = 536880115 peak = 839004160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280711, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (366.47, 162.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675502 reads in 87.126 CPU sec, 2.818 real sec
[M::process] read 675032 sequences (100000226 bp) in 3.266 CPU sec, 3.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281165, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (369.17, 163.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675076 sequences (100000141 bp) in 2.496 CPU sec, 2.586 real sec...
[M::mem_process_seqs] Processed 675032 reads in 87.694 CPU sec, 2.813 real sec
sorting block 33, #reads = 1080936 mem = 536878317 peak = 839004160
[M::process] read 674722 sequences (100000136 bp) in 2.358 CPU sec, 2.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280604, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 357, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (370.34, 163.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674942 sequences (100000128 bp) in 2.010 CPU sec, 2.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280966, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 356, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (370.63, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675076 reads in 154.592 CPU sec, 4.862 real sec
[M::mem_process_seqs] Processed 674722 reads in 102.254 CPU sec, 3.208 real sec
sorting block 34, #reads = 1080877 mem = 536879981 peak = 925552640
[M::process] read 674692 sequences (100000100 bp) in 2.540 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281093, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (369.79, 162.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674492 sequences (100000119 bp) in 2.320 CPU sec, 2.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281906, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (370.30, 161.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674942 reads in 156.636 CPU sec, 4.950 real sec
sorting block 35, #reads = 1080783 mem = 536877517 peak = 925552640
[M::mem_process_seqs] Processed 674692 reads in 104.317 CPU sec, 3.274 real sec
[M::process] read 674872 sequences (100000115 bp) in 2.390 CPU sec, 2.438 real sec...
sorting block 36, #reads = 1080652 mem = 536879744 peak = 936943616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280523, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (370.11, 161.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674402 sequences (100000191 bp) in 2.391 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 280992, 1, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 127, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (218.17, 134.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (365.06, 158.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674492 reads in 159.571 CPU sec, 5.029 real sec
[M::mem_process_seqs] Processed 674872 reads in 103.599 CPU sec, 3.246 real sec
[M::process] read 674224 sequences (100000204 bp) in 2.391 CPU sec, 2.417 real sec...
sorting block 37, #reads = 1079583 mem = 536879480 peak = 936943616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281413, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (368.63, 159.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673974 sequences (100000180 bp) in 2.555 CPU sec, 2.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281594, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (368.60, 159.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674402 reads in 151.609 CPU sec, 4.800 real sec
[M::process] read 674116 sequences (100000119 bp) in 1.666 CPU sec, 1.673 real sec...
[M::mem_process_seqs] Processed 674224 reads in 99.708 CPU sec, 3.135 real sec
sorting block 38, #reads = 1079689 mem = 536879153 peak = 936943616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280959, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 356, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (369.98, 160.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674120 sequences (100000154 bp) in 2.420 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280545, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 354, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (366.85, 157.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674000 sequences (100000066 bp) in 1.840 CPU sec, 1.844 real sec...
[M::mem_process_seqs] Processed 673974 reads in 152.190 CPU sec, 4.809 real sec
[M::mem_process_seqs] Processed 674116 reads in 100.725 CPU sec, 3.181 real sec
sorting block 39, #reads = 1080286 mem = 536879605 peak = 936943616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280469, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 355, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (368.29, 158.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673708 sequences (100000003 bp) in 2.279 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280492, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 358, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (371.25, 160.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673882 sequences (100000065 bp) in 1.640 CPU sec, 1.642 real sec...
[M::mem_process_seqs] Processed 674120 reads in 154.429 CPU sec, 4.891 real sec
sorting block 40, #reads = 1079461 mem = 536880690 peak = 936943616
[M::mem_process_seqs] Processed 674000 reads in 102.446 CPU sec, 3.238 real sec
sorting block 41, #reads = 1079546 mem = 536881006 peak = 971653120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280879, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 354, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (367.57, 158.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674126 sequences (100000207 bp) in 2.491 CPU sec, 2.569 real sec...
[M::process] read 674076 sequences (100000295 bp) in 1.620 CPU sec, 1.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280591, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 353, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (367.34, 157.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673708 reads in 154.356 CPU sec, 4.880 real sec
[M::mem_process_seqs] Processed 673882 reads in 100.783 CPU sec, 3.192 real sec
sorting block 42, #reads = 1078979 mem = 536882367 peak = 971653120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280383, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (364.02, 156.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674168 sequences (100000193 bp) in 2.616 CPU sec, 2.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280584, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (363.17, 156.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674126 reads in 152.680 CPU sec, 4.828 real sec
[M::mem_process_seqs] Processed 674076 reads in 101.119 CPU sec, 3.169 real sec
[M::process] read 674236 sequences (100000166 bp) in 2.438 CPU sec, 2.454 real sec...
sorting block 43, #reads = 1079068 mem = 536879760 peak = 971653120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280492, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (364.12, 157.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674180 sequences (100000152 bp) in 2.775 CPU sec, 2.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280675, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (365.12, 158.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674168 reads in 154.940 CPU sec, 4.898 real sec
[M::mem_process_seqs] Processed 674236 reads in 104.207 CPU sec, 3.264 real sec
[M::process] read 674120 sequences (100000008 bp) in 2.393 CPU sec, 2.422 real sec...
sorting block 44, #reads = 1078978 mem = 536880658 peak = 971653120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280995, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (365.59, 158.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674198 sequences (100000116 bp) in 2.534 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280974, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (364.34, 157.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674180 reads in 149.817 CPU sec, 4.739 real sec
sorting block 45, #reads = 1077580 mem = 536878324 peak = 989134848
[M::mem_process_seqs] Processed 674120 reads in 99.771 CPU sec, 3.129 real sec
[M::process] read 674112 sequences (100000106 bp) in 2.462 CPU sec, 2.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281436, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 349, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (363.76, 157.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1079074 mem = 536876989 peak = 989134848
[M::process] read 674260 sequences (100000175 bp) in 2.730 CPU sec, 2.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280294, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (366.70, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674198 reads in 145.910 CPU sec, 4.619 real sec
[M::mem_process_seqs] Processed 674112 reads in 97.745 CPU sec, 3.071 real sec
[M::process] read 674184 sequences (100000236 bp) in 2.771 CPU sec, 2.846 real sec...
sorting block 47, #reads = 1079056 mem = 536879659 peak = 989134848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281056, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (364.54, 158.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673676 sequences (100000287 bp) in 2.518 CPU sec, 2.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281103, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 353, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (368.10, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674260 reads in 144.846 CPU sec, 4.594 real sec
[M::mem_process_seqs] Processed 674184 reads in 96.703 CPU sec, 3.039 real sec
sorting block 48, #reads = 1079477 mem = 536880406 peak = 989134848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280625, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 357, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (372.15, 162.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674596 sequences (100000072 bp) in 3.390 CPU sec, 3.556 real sec...
[M::mem_process_seqs] Processed 673676 reads in 81.873 CPU sec, 2.685 real sec
sorting block 49, #reads = 1077582 mem = 536878858 peak = 989134848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281091, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (365.61, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674596 reads in 82.661 CPU sec, 2.687 real sec
[M::process] read 678072 sequences (100000132 bp) in 3.071 CPU sec, 3.269 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282786, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (345.43, 157.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677820 sequences (100000216 bp) in 2.126 CPU sec, 2.207 real sec...
[M::mem_process_seqs] Processed 678072 reads in 82.185 CPU sec, 2.640 real sec
sorting block 50, #reads = 1078664 mem = 536879389 peak = 989134848
[M::process] read 677722 sequences (100000011 bp) in 2.125 CPU sec, 2.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282265, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (348.24, 158.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677460 sequences (100000212 bp) in 1.530 CPU sec, 1.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282503, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (348.54, 157.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677820 reads in 146.988 CPU sec, 4.609 real sec
[M::process] read 677004 sequences (100000023 bp) in 1.621 CPU sec, 1.636 real sec...
[M::mem_process_seqs] Processed 677722 reads in 97.549 CPU sec, 3.062 real sec
sorting block 51, #reads = 1081488 mem = 536883492 peak = 989134848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282651, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (349.38, 157.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676870 sequences (100000222 bp) in 2.683 CPU sec, 2.781 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282117, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 335, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (350.38, 156.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677460 reads in 143.481 CPU sec, 4.538 real sec
sorting block 52, #reads = 1081080 mem = 536885144 peak = 989134848
[M::mem_process_seqs] Processed 677004 reads in 96.633 CPU sec, 3.034 real sec
[M::process] read 677018 sequences (100000201 bp) in 2.531 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281656, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 336, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (351.41, 156.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676694 sequences (100000276 bp) in 2.433 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282146, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 336, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (350.88, 156.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676870 reads in 147.056 CPU sec, 4.636 real sec
sorting block 53, #reads = 1080202 mem = 536877919 peak = 989134848
[M::mem_process_seqs] Processed 677018 reads in 98.334 CPU sec, 3.085 real sec
[M::process] read 676234 sequences (100000285 bp) in 2.380 CPU sec, 2.409 real sec...
sorting block 54, #reads = 1080516 mem = 536878908 peak = 989134848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282107, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (353.64, 157.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676034 sequences (100000060 bp) in 2.548 CPU sec, 2.616 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281829, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (356.05, 157.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676694 reads in 147.317 CPU sec, 4.668 real sec
[M::mem_process_seqs] Processed 676234 reads in 99.409 CPU sec, 3.114 real sec
[M::process] read 676056 sequences (100000270 bp) in 2.496 CPU sec, 2.545 real sec...
sorting block 55, #reads = 1080813 mem = 536881693 peak = 989134848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281485, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (358.78, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675634 sequences (100000014 bp) in 2.597 CPU sec, 2.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281759, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (359.53, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676034 reads in 150.049 CPU sec, 4.742 real sec
[M::mem_process_seqs] Processed 676056 reads in 99.772 CPU sec, 3.132 real sec
[M::process] read 675602 sequences (100000079 bp) in 2.462 CPU sec, 2.505 real sec...
sorting block 56, #reads = 1079648 mem = 536880129 peak = 994443264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281783, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (364.20, 161.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675328 sequences (100000112 bp) in 2.191 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281226, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (366.11, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675634 reads in 147.308 CPU sec, 4.666 real sec
[M::process] read 675012 sequences (100000193 bp) in 1.619 CPU sec, 1.622 real sec...
sorting block 57, #reads = 1079820 mem = 536877979 peak = 994443264
[M::mem_process_seqs] Processed 675602 reads in 98.159 CPU sec, 3.094 real sec
sorting block 58, #reads = 1079559 mem = 536878924 peak = 1012801536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281423, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (368.07, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674984 sequences (100000231 bp) in 2.646 CPU sec, 2.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281009, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 355, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (369.84, 163.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674976 sequences (100000151 bp) in 1.580 CPU sec, 1.585 real sec...
[M::mem_process_seqs] Processed 675328 reads in 149.044 CPU sec, 4.704 real sec
[M::mem_process_seqs] Processed 675012 reads in 100.479 CPU sec, 3.168 real sec
sorting block 59, #reads = 1080739 mem = 536881502 peak = 1012801536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281382, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 355, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (369.76, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674828 sequences (100000175 bp) in 2.527 CPU sec, 2.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281075, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (369.20, 163.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674984 reads in 147.676 CPU sec, 4.652 real sec
[M::process] read 674700 sequences (100000099 bp) in 2.092 CPU sec, 2.101 real sec...
[M::mem_process_seqs] Processed 674976 reads in 98.806 CPU sec, 3.103 real sec
sorting block 60, #reads = 1078885 mem = 536877165 peak = 1012801536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281147, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 356, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (369.25, 162.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674714 sequences (100000015 bp) in 2.745 CPU sec, 2.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281316, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 356, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (370.63, 163.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674828 reads in 148.659 CPU sec, 4.691 real sec
[M::mem_process_seqs] Processed 674700 reads in 98.502 CPU sec, 3.086 real sec
[M::process] read 674516 sequences (100000061 bp) in 2.468 CPU sec, 2.491 real sec...
sorting block 61, #reads = 1078921 mem = 536880152 peak = 1012801536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280941, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (369.59, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674762 sequences (100000098 bp) in 2.531 CPU sec, 2.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281193, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (369.87, 162.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674714 reads in 153.464 CPU sec, 4.862 real sec
sorting block 62, #reads = 1080796 mem = 536879051 peak = 1012801536
[M::process] read 674740 sequences (100000065 bp) in 2.247 CPU sec, 2.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280852, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (368.46, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::process] read 674684 sequences (100000267 bp) in 1.777 CPU sec, 1.781 real sec...
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674762 reads in 92.012 CPU sec, 2.909 real sec
sorting block 63, #reads = 1079464 mem = 536880370 peak = 1012801536
[M::mem_process_seqs] Processed 674516 reads in 219.127 CPU sec, 6.931 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280917, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (366.78, 161.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674840 sequences (100000107 bp) in 2.504 CPU sec, 2.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280792, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (367.41, 161.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674740 reads in 173.015 CPU sec, 5.476 real sec
[M::mem_process_seqs] Processed 674684 reads in 104.221 CPU sec, 3.277 real sec
sorting block 64, #reads = 1080030 mem = 536881227 peak = 1012801536
[M::process] read 674580 sequences (100000274 bp) in 2.511 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281160, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (367.48, 162.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674714 sequences (100000294 bp) in 2.563 CPU sec, 2.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280731, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (366.58, 162.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674840 reads in 151.821 CPU sec, 4.794 real sec
sorting block 65, #reads = 1080115 mem = 536882442 peak = 1012801536
[M::mem_process_seqs] Processed 674580 reads in 100.184 CPU sec, 3.143 real sec
[M::process] read 674726 sequences (100000161 bp) in 2.393 CPU sec, 2.406 real sec...
sorting block 66, #reads = 1078563 mem = 536876828 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281175, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (363.08, 159.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674858 sequences (100000022 bp) in 2.607 CPU sec, 2.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280538, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (361.18, 158.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674714 reads in 151.761 CPU sec, 4.806 real sec
[M::mem_process_seqs] Processed 674726 reads in 102.309 CPU sec, 3.211 real sec
[M::process] read 674518 sequences (100000120 bp) in 2.493 CPU sec, 2.533 real sec...
sorting block 67, #reads = 1079242 mem = 536880141 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280836, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (363.29, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674804 sequences (100000089 bp) in 2.783 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280604, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (365.46, 160.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674858 reads in 151.165 CPU sec, 4.785 real sec
[M::mem_process_seqs] Processed 674518 reads in 100.783 CPU sec, 3.166 real sec
sorting block 68, #reads = 1079262 mem = 536878896 peak = 1043718144
[M::process] read 674772 sequences (100000026 bp) in 2.577 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280819, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (362.38, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674362 sequences (100000103 bp) in 2.565 CPU sec, 2.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281115, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (361.99, 157.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674804 reads in 149.214 CPU sec, 4.729 real sec
[M::mem_process_seqs] Processed 674772 reads in 99.627 CPU sec, 3.125 real sec
sorting block 69, #reads = 1077443 mem = 536878871 peak = 1043718144
[M::process] read 674488 sequences (100000051 bp) in 2.573 CPU sec, 2.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280476, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (364.78, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674314 sequences (100000003 bp) in 2.646 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280776, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (367.05, 160.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674362 reads in 145.458 CPU sec, 4.609 real sec
sorting block 70, #reads = 1077684 mem = 536881564 peak = 1043718144
[M::mem_process_seqs] Processed 674488 reads in 96.911 CPU sec, 3.043 real sec
[M::process] read 674042 sequences (100000266 bp) in 2.887 CPU sec, 2.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280334, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (366.84, 160.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 71, #reads = 1079605 mem = 536877591 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280809, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 353, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (368.54, 161.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673994 sequences (100000037 bp) in 2.822 CPU sec, 2.913 real sec...
[M::mem_process_seqs] Processed 674314 reads in 146.518 CPU sec, 4.653 real sec
[M::mem_process_seqs] Processed 674042 reads in 97.465 CPU sec, 3.071 real sec
sorting block 72, #reads = 1078368 mem = 536878614 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280942, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 353, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (367.99, 160.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674048 sequences (100000080 bp) in 2.923 CPU sec, 3.026 real sec...
[M::process] read 673988 sequences (100000231 bp) in 2.168 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280700, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 351, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (366.20, 159.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674048 reads in 78.926 CPU sec, 2.470 real sec
sorting block 73, #reads = 1079431 mem = 536879831 peak = 1043718144
[M::mem_process_seqs] Processed 673994 reads in 207.886 CPU sec, 6.615 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280477, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (368.08, 161.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674910 sequences (100000142 bp) in 2.794 CPU sec, 2.862 real sec...
[M::mem_process_seqs] Processed 673988 reads in 100.169 CPU sec, 3.200 real sec
sorting block 74, #reads = 1078456 mem = 536879426 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281230, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (363.72, 159.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677704 sequences (100000011 bp) in 2.788 CPU sec, 2.899 real sec...
[M::mem_process_seqs] Processed 674910 reads in 90.326 CPU sec, 2.900 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282359, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (350.97, 161.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677244 sequences (100000123 bp) in 2.657 CPU sec, 2.717 real sec...
[M::mem_process_seqs] Processed 677704 reads in 83.542 CPU sec, 2.667 real sec
sorting block 75, #reads = 1077573 mem = 536878084 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281510, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (353.47, 161.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677122 sequences (100000078 bp) in 2.598 CPU sec, 2.695 real sec...
[M::mem_process_seqs] Processed 677244 reads in 82.234 CPU sec, 2.641 real sec
sorting block 76, #reads = 1083378 mem = 536880210 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282149, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (355.33, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677010 sequences (100000286 bp) in 2.549 CPU sec, 2.610 real sec...
[M::mem_process_seqs] Processed 677122 reads in 82.007 CPU sec, 2.621 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281263, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (356.00, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676672 sequences (100000061 bp) in 2.569 CPU sec, 2.633 real sec...
[M::mem_process_seqs] Processed 677010 reads in 80.455 CPU sec, 2.580 real sec
sorting block 77, #reads = 1082449 mem = 536880201 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281496, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (358.10, 162.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676310 sequences (100000069 bp) in 2.536 CPU sec, 2.603 real sec...
[M::mem_process_seqs] Processed 676672 reads in 80.231 CPU sec, 2.566 real sec
sorting block 78, #reads = 1082196 mem = 536882664 peak = 1043718144
[M::process] read 676460 sequences (100000045 bp) in 1.986 CPU sec, 2.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281657, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (358.10, 162.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676374 sequences (100000166 bp) in 1.480 CPU sec, 1.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281204, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 343, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (359.42, 162.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676310 reads in 141.775 CPU sec, 4.445 real sec
[M::mem_process_seqs] Processed 676460 reads in 94.356 CPU sec, 2.952 real sec
[M::process] read 675958 sequences (100000097 bp) in 1.617 CPU sec, 1.636 real sec...
sorting block 79, #reads = 1082291 mem = 536880504 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281312, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (360.07, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675912 sequences (100000055 bp) in 2.567 CPU sec, 2.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282015, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (361.41, 161.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676374 reads in 140.406 CPU sec, 4.451 real sec
[M::mem_process_seqs] Processed 675958 reads in 92.538 CPU sec, 2.907 real sec
sorting block 80, #reads = 1082024 mem = 536880070 peak = 1043718144
[M::process] read 675636 sequences (100000252 bp) in 2.303 CPU sec, 2.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281774, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (362.10, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675486 sequences (100000135 bp) in 2.321 CPU sec, 2.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281153, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (363.46, 162.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675912 reads in 143.154 CPU sec, 4.515 real sec
[M::mem_process_seqs] Processed 675636 reads in 94.975 CPU sec, 2.979 real sec
[M::process] read 675568 sequences (100000234 bp) in 2.141 CPU sec, 2.166 real sec...
sorting block 81, #reads = 1082324 mem = 536879678 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281025, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (365.65, 163.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675246 sequences (100000145 bp) in 2.518 CPU sec, 2.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281689, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (367.00, 164.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675486 reads in 144.180 CPU sec, 4.573 real sec
sorting block 82, #reads = 1080219 mem = 536881848 peak = 1043718144
[M::mem_process_seqs] Processed 675568 reads in 96.775 CPU sec, 3.036 real sec
[M::process] read 675294 sequences (100000289 bp) in 2.539 CPU sec, 2.570 real sec...
sorting block 83, #reads = 1081432 mem = 536878120 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282336, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (368.39, 163.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675208 sequences (100000060 bp) in 2.204 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281598, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (369.94, 165.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (397, 522, 743)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435)
[M::mem_pestat] mean and std.dev: (488.36, 206.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1781)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675246 reads in 145.199 CPU sec, 4.586 real sec
[M::process] read 674986 sequences (100000074 bp) in 1.696 CPU sec, 1.701 real sec...
[M::mem_process_seqs] Processed 675294 reads in 96.366 CPU sec, 3.025 real sec
sorting block 84, #reads = 1081784 mem = 536880598 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280852, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (369.85, 165.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674946 sequences (100000098 bp) in 2.607 CPU sec, 2.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281450, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 355, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (371.20, 166.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675208 reads in 145.184 CPU sec, 4.590 real sec
[M::mem_process_seqs] Processed 674986 reads in 96.928 CPU sec, 3.039 real sec
[M::process] read 674822 sequences (100000061 bp) in 2.129 CPU sec, 2.159 real sec...
sorting block 85, #reads = 1080701 mem = 536877810 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280850, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 356, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (372.61, 167.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674956 sequences (100000129 bp) in 2.583 CPU sec, 2.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281432, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 356, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (372.65, 166.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674946 reads in 139.673 CPU sec, 4.415 real sec
[M::mem_process_seqs] Processed 674822 reads in 91.495 CPU sec, 2.865 real sec
sorting block 86, #reads = 1080314 mem = 536879656 peak = 1043718144
[M::process] read 674778 sequences (100000011 bp) in 2.574 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281212, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 355, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (371.27, 166.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675118 sequences (100000038 bp) in 2.472 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281302, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (368.43, 164.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674956 reads in 143.400 CPU sec, 4.549 real sec
sorting block 87, #reads = 1080599 mem = 536880843 peak = 1043718144
[M::mem_process_seqs] Processed 674778 reads in 96.075 CPU sec, 3.017 real sec
[M::process] read 675280 sequences (100000112 bp) in 2.695 CPU sec, 2.748 real sec...
sorting block 88, #reads = 1080792 mem = 536883318 peak = 1043718144
[M::process] read 675104 sequences (100000004 bp) in 2.433 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281364, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (366.32, 164.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675280 reads in 81.136 CPU sec, 2.548 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280703, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (367.99, 164.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675378 sequences (100000000 bp) in 2.413 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281274, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (366.45, 164.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675118 reads in 228.705 CPU sec, 7.220 real sec
sorting block 89, #reads = 1080168 mem = 536881826 peak = 1043718144
[M::mem_process_seqs] Processed 675104 reads in 115.024 CPU sec, 3.621 real sec
[M::process] read 675494 sequences (100000150 bp) in 2.417 CPU sec, 2.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281006, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (364.69, 164.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 90, #reads = 1079347 mem = 536880495 peak = 1043718144
[M::process] read 675324 sequences (100000175 bp) in 2.281 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281137, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (363.49, 163.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675378 reads in 149.541 CPU sec, 4.731 real sec
[M::mem_process_seqs] Processed 675494 reads in 99.422 CPU sec, 3.118 real sec
[M::process] read 675194 sequences (100000150 bp) in 2.304 CPU sec, 2.323 real sec...
sorting block 91, #reads = 1080197 mem = 536880002 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281262, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (362.92, 162.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675246 sequences (100000200 bp) in 2.729 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281084, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (362.99, 162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675324 reads in 147.375 CPU sec, 4.662 real sec
[M::mem_process_seqs] Processed 675194 reads in 98.968 CPU sec, 3.107 real sec
[M::process] read 675172 sequences (100000255 bp) in 2.620 CPU sec, 2.665 real sec...
sorting block 92, #reads = 1079074 mem = 536877784 peak = 1043718144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280990, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (363.01, 162.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675076 sequences (100000106 bp) in 2.514 CPU sec, 2.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281374, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (361.65, 161.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675246 reads in 149.026 CPU sec, 4.715 real sec
[M::mem_process_seqs] Processed 675172 reads in 98.564 CPU sec, 3.089 real sec
sorting block 93, #reads = 1079329 mem = 536878903 peak = 1043718144
[M::process] read 675026 sequences (100000126 bp) in 2.498 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280772, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 342, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (359.17, 158.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674736 sequences (100000066 bp) in 2.728 CPU sec, 2.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281429, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 344, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (360.90, 159.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675076 reads in 145.202 CPU sec, 4.599 real sec
sorting block 94, #reads = 1080140 mem = 536878390 peak = 1043718144
[M::mem_process_seqs] Processed 675026 reads in 96.919 CPU sec, 3.044 real sec
[M::process] read 674730 sequences (100000135 bp) in 2.613 CPU sec, 2.679 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281010, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (363.77, 161.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 95, #reads = 1079606 mem = 536881698 peak = 1043718144
[M::process] read 674662 sequences (100000268 bp) in 1.992 CPU sec, 2.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281553, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (364.37, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674736 reads in 145.347 CPU sec, 4.600 real sec
[M::process] read 674510 sequences (100000239 bp) in 1.698 CPU sec, 1.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281247, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (363.57, 159.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674662 reads in 78.527 CPU sec, 2.474 real sec
[M::process] read 674658 sequences (100000223 bp) in 2.381 CPU sec, 2.395 real sec...
sorting block 96, #reads = 1079972 mem = 536884007 peak = 1043718144
[M::mem_process_seqs] Processed 674730 reads in 205.162 CPU sec, 6.467 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281256, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (367.81, 162.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674378 sequences (100000290 bp) in 2.340 CPU sec, 2.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281327, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (367.52, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674510 reads in 158.457 CPU sec, 4.998 real sec
sorting block 97, #reads = 1079368 mem = 536877367 peak = 1046466560
[M::mem_process_seqs] Processed 674658 reads in 95.860 CPU sec, 3.014 real sec
[M::process] read 674528 sequences (100000217 bp) in 2.194 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281284, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (369.11, 163.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 98, #reads = 1079157 mem = 536883166 peak = 1082400768
[M::process] read 674130 sequences (100000183 bp) in 2.749 CPU sec, 2.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280928, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (366.49, 162.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674378 reads in 142.756 CPU sec, 4.516 real sec
[M::mem_process_seqs] Processed 674528 reads in 96.437 CPU sec, 3.027 real sec
[M::process] read 678578 sequences (100000185 bp) in 2.114 CPU sec, 2.171 real sec...
sorting block 99, #reads = 1078332 mem = 536878925 peak = 1082400768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281211, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (368.63, 162.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679716 sequences (100000149 bp) in 2.574 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282828, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (348.82, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674130 reads in 151.601 CPU sec, 4.806 real sec
[M::mem_process_seqs] Processed 678578 reads in 107.982 CPU sec, 3.396 real sec
sorting block 100, #reads = 1078762 mem = 536878276 peak = 1082400768
[M::process] read 680068 sequences (100000006 bp) in 2.482 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282841, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (344.61, 165.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679570 sequences (100000077 bp) in 2.146 CPU sec, 2.200 real sec...
[M::process] read 678880 sequences (100000202 bp) in 1.560 CPU sec, 1.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282708, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (339.03, 161.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679716 reads in 161.351 CPU sec, 5.112 real sec
sorting block 101, #reads = 1077703 mem = 536886779 peak = 1082400768
[M::mem_process_seqs] Processed 680068 reads in 107.228 CPU sec, 3.393 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283475, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (342.00, 161.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678676 sequences (100000046 bp) in 2.668 CPU sec, 2.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282672, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (347.12, 163.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679570 reads in 162.631 CPU sec, 5.129 real sec
sorting block 102, #reads = 1078656 mem = 536878052 peak = 1082400768
[M::process] read 678216 sequences (100000063 bp) in 2.257 CPU sec, 2.267 real sec...
[M::mem_process_seqs] Processed 678880 reads in 105.623 CPU sec, 3.315 real sec
sorting block 103, #reads = 1078846 mem = 536878505 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281896, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 328, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (349.22, 163.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678456 sequences (100000094 bp) in 2.565 CPU sec, 2.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282389, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (349.97, 163.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678676 reads in 160.865 CPU sec, 5.072 real sec
[M::process] read 678702 sequences (100000253 bp) in 2.249 CPU sec, 2.255 real sec...
[M::mem_process_seqs] Processed 678216 reads in 104.702 CPU sec, 3.295 real sec
sorting block 104, #reads = 1080971 mem = 536879820 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281847, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.15, 163.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679312 sequences (100000088 bp) in 2.627 CPU sec, 2.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282268, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (346.56, 162.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678456 reads in 157.783 CPU sec, 4.985 real sec
[M::mem_process_seqs] Processed 678702 reads in 103.337 CPU sec, 3.241 real sec
[M::process] read 677628 sequences (100000012 bp) in 2.465 CPU sec, 2.481 real sec...
sorting block 105, #reads = 1080299 mem = 536879809 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282666, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (340.79, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677224 sequences (100000205 bp) in 2.273 CPU sec, 2.342 real sec...
[M::process] read 677408 sequences (100000183 bp) in 1.680 CPU sec, 1.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282114, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (353.70, 163.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679312 reads in 154.101 CPU sec, 4.877 real sec
sorting block 106, #reads = 1079896 mem = 536886813 peak = 1112723456
[M::mem_process_seqs] Processed 677628 reads in 99.926 CPU sec, 3.158 real sec
sorting block 107, #reads = 1079587 mem = 536875260 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281793, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (355.45, 163.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676818 sequences (100000109 bp) in 2.703 CPU sec, 2.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282157, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (355.22, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677224 reads in 153.566 CPU sec, 4.854 real sec
[M::mem_process_seqs] Processed 677408 reads in 101.279 CPU sec, 3.172 real sec
[M::process] read 676522 sequences (100000171 bp) in 2.277 CPU sec, 2.313 real sec...
sorting block 108, #reads = 1079467 mem = 536881875 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281674, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (360.44, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676678 sequences (100000246 bp) in 2.259 CPU sec, 2.336 real sec...
[M::process] read 676758 sequences (100000164 bp) in 1.630 CPU sec, 1.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281591, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (361.63, 164.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676818 reads in 163.157 CPU sec, 5.174 real sec
[M::mem_process_seqs] Processed 676522 reads in 107.960 CPU sec, 3.420 real sec
sorting block 109, #reads = 1077210 mem = 536890168 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281923, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 342, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (358.60, 162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678126 sequences (100000083 bp) in 2.449 CPU sec, 2.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281312, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 342, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (358.23, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677080 sequences (100000041 bp) in 1.690 CPU sec, 1.686 real sec...
[M::mem_process_seqs] Processed 676678 reads in 154.930 CPU sec, 4.896 real sec
[M::mem_process_seqs] Processed 676758 reads in 101.233 CPU sec, 3.200 real sec
sorting block 110, #reads = 1077110 mem = 536883533 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282244, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (346.35, 160.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675912 sequences (100000005 bp) in 2.991 CPU sec, 3.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282167, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (351.25, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676218 sequences (100000024 bp) in 1.720 CPU sec, 1.719 real sec...
[M::mem_process_seqs] Processed 678126 reads in 163.334 CPU sec, 5.164 real sec
sorting block 111, #reads = 1077148 mem = 536884929 peak = 1112723456
[M::mem_process_seqs] Processed 677080 reads in 109.344 CPU sec, 3.453 real sec
sorting block 112, #reads = 1078408 mem = 536882598 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281579, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 348, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (362.99, 162.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676532 sequences (100000273 bp) in 2.719 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281895, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 348, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (364.42, 164.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675912 reads in 166.663 CPU sec, 5.260 real sec
[M::process] read 675670 sequences (100000263 bp) in 2.347 CPU sec, 2.354 real sec...
[M::mem_process_seqs] Processed 676218 reads in 108.919 CPU sec, 3.416 real sec
sorting block 113, #reads = 1077491 mem = 536877931 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281288, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (358.87, 161.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675732 sequences (100000178 bp) in 2.904 CPU sec, 2.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281469, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 352, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (366.13, 163.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676532 reads in 166.033 CPU sec, 5.239 real sec
[M::process] read 675190 sequences (100000134 bp) in 2.327 CPU sec, 2.332 real sec...
[M::mem_process_seqs] Processed 675670 reads in 110.304 CPU sec, 3.467 real sec
sorting block 114, #reads = 1076203 mem = 536879775 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281720, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (360.62, 158.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674852 sequences (100000243 bp) in 2.735 CPU sec, 2.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281546, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (362.72, 158.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675732 reads in 167.824 CPU sec, 5.293 real sec
[M::process] read 674770 sequences (100000126 bp) in 2.309 CPU sec, 2.315 real sec...
[M::mem_process_seqs] Processed 675190 reads in 109.199 CPU sec, 3.434 real sec
sorting block 115, #reads = 1075210 mem = 536879933 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280849, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (366.41, 159.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674570 sequences (100000176 bp) in 2.958 CPU sec, 3.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281166, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (365.59, 158.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674852 reads in 168.539 CPU sec, 5.311 real sec
[M::process] read 674160 sequences (100000187 bp) in 2.377 CPU sec, 2.378 real sec...
sorting block 116, #reads = 1075949 mem = 536878475 peak = 1112723456
[M::mem_process_seqs] Processed 674770 reads in 112.396 CPU sec, 3.527 real sec
sorting block 117, #reads = 1074880 mem = 536881920 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281586, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (366.25, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674152 sequences (100000166 bp) in 2.438 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280871, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (369.50, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674540 sequences (100000122 bp) in 2.260 CPU sec, 2.261 real sec...
[M::mem_process_seqs] Processed 674570 reads in 168.066 CPU sec, 5.309 real sec
[M::mem_process_seqs] Processed 674160 reads in 110.208 CPU sec, 3.471 real sec
sorting block 118, #reads = 1074845 mem = 536878150 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280941, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (369.60, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674210 sequences (100000038 bp) in 2.966 CPU sec, 3.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281645, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (367.39, 159.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674152 reads in 165.974 CPU sec, 5.232 real sec
[M::process] read 674262 sequences (100000113 bp) in 2.382 CPU sec, 2.391 real sec...
[M::mem_process_seqs] Processed 674540 reads in 109.626 CPU sec, 3.439 real sec
sorting block 119, #reads = 1075094 mem = 536881030 peak = 1112723456
[M::process] read 674036 sequences (100000137 bp) in 2.732 CPU sec, 2.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280955, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (369.48, 160.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674262 reads in 100.388 CPU sec, 3.145 real sec
[M::process] read 674002 sequences (100000181 bp) in 2.349 CPU sec, 2.354 real sec...
sorting block 120, #reads = 1075415 mem = 536879976 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281204, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (368.68, 160.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281118, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 357, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (372.06, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674210 reads in 278.922 CPU sec, 8.802 real sec
[M::process] read 675196 sequences (100000037 bp) in 2.677 CPU sec, 2.680 real sec...
[M::mem_process_seqs] Processed 674036 reads in 143.945 CPU sec, 4.542 real sec
sorting block 121, #reads = 1074438 mem = 536878402 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281486, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 360, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (374.43, 163.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682306 sequences (100000048 bp) in 2.894 CPU sec, 3.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281305, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (368.71, 164.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681730 sequences (100000111 bp) in 2.370 CPU sec, 2.370 real sec...
[M::mem_process_seqs] Processed 674002 reads in 184.200 CPU sec, 5.811 real sec
sorting block 122, #reads = 1074890 mem = 536878354 peak = 1112723456
[M::mem_process_seqs] Processed 675196 reads in 126.495 CPU sec, 4.001 real sec
[M::process] read 682332 sequences (100000152 bp) in 3.063 CPU sec, 3.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283632, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 302, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (322.14, 149.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681730 sequences (100000211 bp) in 2.430 CPU sec, 2.431 real sec...
[M::mem_process_seqs] Processed 681730 reads in 109.066 CPU sec, 3.432 real sec
sorting block 123, #reads = 1073961 mem = 536879351 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284026, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 299, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (319.94, 148.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680286 sequences (100000089 bp) in 2.105 CPU sec, 2.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283932, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 299, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (317.70, 145.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682306 reads in 313.091 CPU sec, 9.895 real sec
sorting block 124, #reads = 1075339 mem = 536881941 peak = 1112723456
[M::mem_process_seqs] Processed 682332 reads in 161.522 CPU sec, 5.143 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283734, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 303, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (320.16, 145.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680294 sequences (100000269 bp) in 2.831 CPU sec, 2.927 real sec...
[M::process] read 679484 sequences (100000264 bp) in 1.840 CPU sec, 1.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282943, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (329.60, 149.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681730 reads in 203.440 CPU sec, 6.446 real sec
sorting block 125, #reads = 1072616 mem = 536879580 peak = 1112723456
[M::mem_process_seqs] Processed 680286 reads in 133.832 CPU sec, 4.254 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283304, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (330.66, 150.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679094 sequences (100000237 bp) in 2.572 CPU sec, 2.670 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283448, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (336.73, 153.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678154 sequences (100000059 bp) in 2.350 CPU sec, 2.345 real sec...
[M::mem_process_seqs] Processed 680294 reads in 191.263 CPU sec, 6.057 real sec
sorting block 126, #reads = 1073696 mem = 536883143 peak = 1112723456
[M::mem_process_seqs] Processed 679484 reads in 126.682 CPU sec, 4.000 real sec
sorting block 127, #reads = 1074910 mem = 536879239 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283446, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (338.51, 153.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677852 sequences (100000281 bp) in 3.158 CPU sec, 3.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282681, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (344.68, 155.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677736 sequences (100000199 bp) in 2.000 CPU sec, 1.996 real sec...
[M::mem_process_seqs] Processed 679094 reads in 196.479 CPU sec, 6.223 real sec
[M::mem_process_seqs] Processed 678154 reads in 126.878 CPU sec, 4.009 real sec
sorting block 128, #reads = 1074505 mem = 536878811 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282929, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (346.98, 156.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677712 sequences (100000149 bp) in 3.062 CPU sec, 3.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282292, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (348.92, 157.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677852 reads in 182.443 CPU sec, 5.749 real sec
[M::process] read 677234 sequences (100000202 bp) in 2.406 CPU sec, 2.414 real sec...
[M::mem_process_seqs] Processed 677736 reads in 118.568 CPU sec, 3.733 real sec
sorting block 129, #reads = 1075266 mem = 536881251 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282214, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (348.71, 156.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677616 sequences (100000002 bp) in 2.780 CPU sec, 2.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282519, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (351.87, 157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677202 sequences (100000296 bp) in 2.260 CPU sec, 2.256 real sec...
[M::mem_process_seqs] Processed 677712 reads in 192.413 CPU sec, 6.086 real sec
sorting block 130, #reads = 1075772 mem = 536880596 peak = 1112723456
[M::mem_process_seqs] Processed 677234 reads in 130.139 CPU sec, 4.107 real sec
sorting block 131, #reads = 1071480 mem = 536878729 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282181, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.11, 155.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677098 sequences (100000201 bp) in 2.988 CPU sec, 3.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282042, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (349.29, 156.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676820 sequences (100000011 bp) in 2.320 CPU sec, 2.324 real sec...
[M::mem_process_seqs] Processed 677202 reads in 182.397 CPU sec, 5.750 real sec
[M::mem_process_seqs] Processed 677616 reads in 204.538 CPU sec, 6.474 real sec
sorting block 132, #reads = 1075228 mem = 536880094 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282424, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (350.90, 156.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676572 sequences (100000039 bp) in 2.268 CPU sec, 2.339 real sec...
[M::process] read 676476 sequences (100000065 bp) in 1.930 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281850, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (351.58, 156.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677098 reads in 180.790 CPU sec, 5.735 real sec
[M::mem_process_seqs] Processed 676820 reads in 119.060 CPU sec, 3.782 real sec
sorting block 133, #reads = 1074417 mem = 536883561 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282482, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (355.62, 157.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676624 sequences (100000230 bp) in 3.100 CPU sec, 3.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282135, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (356.23, 158.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676362 sequences (100000177 bp) in 1.950 CPU sec, 1.950 real sec...
[M::mem_process_seqs] Processed 676572 reads in 183.387 CPU sec, 5.793 real sec
sorting block 134, #reads = 1074732 mem = 536883752 peak = 1112723456
[M::mem_process_seqs] Processed 676476 reads in 123.452 CPU sec, 3.905 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281838, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (355.40, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676110 sequences (100000197 bp) in 2.663 CPU sec, 2.725 real sec...
[M::process] read 675234 sequences (100000205 bp) in 2.240 CPU sec, 2.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281550, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (356.40, 158.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676624 reads in 183.626 CPU sec, 5.804 real sec
sorting block 135, #reads = 1074386 mem = 536879240 peak = 1112723456
[M::mem_process_seqs] Processed 676362 reads in 125.374 CPU sec, 3.965 real sec
sorting block 136, #reads = 1074941 mem = 536879016 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281368, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 347, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (361.53, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675248 sequences (100000198 bp) in 3.037 CPU sec, 3.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281353, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (366.21, 160.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674606 sequences (100000181 bp) in 2.400 CPU sec, 2.393 real sec...
[M::mem_process_seqs] Processed 676110 reads in 181.058 CPU sec, 5.702 real sec
[M::mem_process_seqs] Processed 675234 reads in 120.222 CPU sec, 3.785 real sec
sorting block 137, #reads = 1075890 mem = 536883783 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281098, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 356, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (369.56, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674470 sequences (100000079 bp) in 2.368 CPU sec, 2.406 real sec...
[M::process] read 674874 sequences (100000296 bp) in 1.776 CPU sec, 1.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281144, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 358, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (371.78, 162.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675248 reads in 180.589 CPU sec, 5.738 real sec
[M::mem_process_seqs] Processed 674606 reads in 121.757 CPU sec, 3.874 real sec
sorting block 138, #reads = 1076661 mem = 536883974 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281783, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 357, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (371.84, 163.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674728 sequences (100000214 bp) in 3.076 CPU sec, 3.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281483, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (369.01, 163.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674814 sequences (100000260 bp) in 2.340 CPU sec, 2.348 real sec...
[M::mem_process_seqs] Processed 674470 reads in 181.060 CPU sec, 5.705 real sec
sorting block 139, #reads = 1075406 mem = 536878115 peak = 1112723456
[M::mem_process_seqs] Processed 674874 reads in 119.120 CPU sec, 3.747 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281038, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 355, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (372.15, 166.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674620 sequences (100000053 bp) in 3.112 CPU sec, 3.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281366, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 356, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (372.24, 166.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675166 sequences (100000218 bp) in 2.320 CPU sec, 2.323 real sec...
[M::mem_process_seqs] Processed 674728 reads in 183.871 CPU sec, 5.799 real sec
sorting block 140, #reads = 1076162 mem = 536876796 peak = 1112723456
[M::mem_process_seqs] Processed 674814 reads in 124.446 CPU sec, 3.923 real sec
sorting block 141, #reads = 1075996 mem = 536880358 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281494, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 356, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (372.01, 165.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675200 sequences (100000301 bp) in 3.038 CPU sec, 3.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281721, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 351, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (369.44, 167.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675004 sequences (100000151 bp) in 2.440 CPU sec, 2.434 real sec...
[M::mem_process_seqs] Processed 674620 reads in 185.374 CPU sec, 5.843 real sec
[M::mem_process_seqs] Processed 675166 reads in 126.523 CPU sec, 3.988 real sec
sorting block 142, #reads = 1074406 mem = 536878238 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281768, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (369.10, 168.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679866 sequences (100000186 bp) in 2.627 CPU sec, 2.701 real sec...
[M::process] read 680266 sequences (100000192 bp) in 2.340 CPU sec, 2.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280966, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 353, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (370.87, 167.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675200 reads in 199.702 CPU sec, 6.321 real sec
sorting block 143, #reads = 1074318 mem = 536877274 peak = 1112723456
[M::mem_process_seqs] Processed 675004 reads in 138.832 CPU sec, 4.393 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282073, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 316, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (335.83, 155.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680366 sequences (100000163 bp) in 3.124 CPU sec, 3.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283027, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (333.61, 155.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679424 sequences (100000070 bp) in 2.337 CPU sec, 2.344 real sec...
[M::mem_process_seqs] Processed 679866 reads in 193.493 CPU sec, 6.100 real sec
sorting block 144, #reads = 1065625 mem = 536879127 peak = 1112723456
[M::mem_process_seqs] Processed 680266 reads in 122.351 CPU sec, 3.871 real sec
[M::process] read 678682 sequences (100000054 bp) in 2.876 CPU sec, 2.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282600, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (339.21, 157.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678498 sequences (100000166 bp) in 2.270 CPU sec, 2.269 real sec...
[M::mem_process_seqs] Processed 679424 reads in 98.112 CPU sec, 3.076 real sec
sorting block 145, #reads = 1078301 mem = 536885612 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283141, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (331.08, 153.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282847, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (341.47, 156.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677964 sequences (100000018 bp) in 2.029 CPU sec, 2.032 real sec...
[M::mem_process_seqs] Processed 680366 reads in 275.188 CPU sec, 8.693 real sec
[M::mem_process_seqs] Processed 678682 reads in 136.515 CPU sec, 4.330 real sec
sorting block 146, #reads = 1078568 mem = 536878612 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282288, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (343.94, 156.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678070 sequences (100000279 bp) in 2.954 CPU sec, 3.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282077, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (347.34, 158.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678498 reads in 173.309 CPU sec, 5.469 real sec
[M::process] read 677680 sequences (100000088 bp) in 2.319 CPU sec, 2.324 real sec...
sorting block 147, #reads = 1079034 mem = 536879981 peak = 1112723456
[M::mem_process_seqs] Processed 677964 reads in 114.417 CPU sec, 3.597 real sec
sorting block 148, #reads = 1078906 mem = 536876944 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282249, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (348.40, 159.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677534 sequences (100000149 bp) in 2.886 CPU sec, 2.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282500, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (349.51, 159.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678070 reads in 174.496 CPU sec, 5.506 real sec
[M::process] read 677270 sequences (100000171 bp) in 2.443 CPU sec, 2.450 real sec...
[M::mem_process_seqs] Processed 677680 reads in 116.120 CPU sec, 3.643 real sec
sorting block 149, #reads = 1080200 mem = 536878960 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282335, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (351.35, 159.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676890 sequences (100000165 bp) in 2.941 CPU sec, 2.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281949, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (353.87, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677534 reads in 172.724 CPU sec, 5.457 real sec
[M::process] read 676802 sequences (100000082 bp) in 2.404 CPU sec, 2.415 real sec...
[M::mem_process_seqs] Processed 677270 reads in 113.388 CPU sec, 3.557 real sec
sorting block 150, #reads = 1079465 mem = 536879627 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282343, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (355.53, 160.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676868 sequences (100000265 bp) in 2.763 CPU sec, 2.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282673, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (356.20, 159.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676890 reads in 172.456 CPU sec, 5.456 real sec
[M::process] read 676636 sequences (100000095 bp) in 2.539 CPU sec, 2.546 real sec...
[M::mem_process_seqs] Processed 676802 reads in 113.968 CPU sec, 3.574 real sec
sorting block 151, #reads = 1079231 mem = 536878274 peak = 1112723456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281821, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (355.90, 159.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676702 sequences (100000165 bp) in 2.940 CPU sec, 3.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282127, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (357.74, 160.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676868 reads in 176.631 CPU sec, 5.582 real sec
[M::process] read 676412 sequences (100000009 bp) in 2.358 CPU sec, 2.371 real sec...
sorting block 152, #reads = 1078530 mem = 536881877 peak = 1112723456
[M::mem_process_seqs] Processed 676636 reads in 117.937 CPU sec, 3.709 real sec
sorting block 153, #reads = 1077783 mem = 536888612 peak = 1129177088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282003, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 343, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (358.20, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676188 sequences (100000268 bp) in 2.798 CPU sec, 2.868 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281889, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (358.74, 160.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675768 sequences (100000226 bp) in 2.340 CPU sec, 2.337 real sec...
[M::mem_process_seqs] Processed 676702 reads in 178.476 CPU sec, 5.632 real sec
[M::mem_process_seqs] Processed 676412 reads in 118.182 CPU sec, 3.729 real sec
sorting block 154, #reads = 1077984 mem = 536878918 peak = 1129177088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281323, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (360.20, 160.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675786 sequences (100000268 bp) in 2.991 CPU sec, 3.061 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281941, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 349, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (363.94, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675538 sequences (100000212 bp) in 1.800 CPU sec, 1.795 real sec...
[M::mem_process_seqs] Processed 676188 reads in 177.589 CPU sec, 5.618 real sec
[M::mem_process_seqs] Processed 675768 reads in 114.926 CPU sec, 3.638 real sec
sorting block 155, #reads = 1077493 mem = 536880643 peak = 1129177088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281560, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (365.47, 163.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675708 sequences (100000261 bp) in 2.218 CPU sec, 2.290 real sec...
[M::process] read 675794 sequences (100000133 bp) in 1.899 CPU sec, 1.904 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280983, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 351, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (365.72, 163.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675786 reads in 176.597 CPU sec, 5.606 real sec
sorting block 156, #reads = 1077231 mem = 536883693 peak = 1129177088
[M::mem_process_seqs] Processed 675538 reads in 117.456 CPU sec, 3.727 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281834, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (364.66, 161.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675514 sequences (100000245 bp) in 3.195 CPU sec, 3.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281744, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 349, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (363.58, 161.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675580 sequences (100000219 bp) in 2.417 CPU sec, 2.423 real sec...
[M::mem_process_seqs] Processed 675708 reads in 191.669 CPU sec, 6.069 real sec
sorting block 157, #reads = 1076207 mem = 536879658 peak = 1129177088
[M::mem_process_seqs] Processed 675794 reads in 129.389 CPU sec, 4.098 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281957, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (362.44, 159.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 158, #reads = 1075551 mem = 536876547 peak = 1129177088
[M::process] read 675540 sequences (100000155 bp) in 2.862 CPU sec, 3.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281500, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (360.22, 158.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675514 reads in 195.349 CPU sec, 6.249 real sec
[M::process] read 675410 sequences (100000090 bp) in 2.422 CPU sec, 2.469 real sec...
[M::mem_process_seqs] Processed 675580 reads in 131.676 CPU sec, 4.173 real sec
sorting block 159, #reads = 1076031 mem = 536879515 peak = 1129177088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281534, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (361.68, 158.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675384 sequences (100000291 bp) in 3.113 CPU sec, 3.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281852, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (361.78, 158.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675540 reads in 195.192 CPU sec, 6.211 real sec
[M::process] read 675506 sequences (100000234 bp) in 2.455 CPU sec, 2.471 real sec...
[M::mem_process_seqs] Processed 675410 reads in 129.873 CPU sec, 4.102 real sec
sorting block 160, #reads = 1075181 mem = 536877653 peak = 1129177088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281802, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (360.89, 157.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675548 sequences (100000019 bp) in 2.638 CPU sec, 2.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281456, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (359.36, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675432 sequences (100000090 bp) in 2.469 CPU sec, 2.487 real sec...
[M::mem_process_seqs] Processed 675384 reads in 198.808 CPU sec, 6.339 real sec
sorting block 161, #reads = 1076064 mem = 536882726 peak = 1129177088
[M::mem_process_seqs] Processed 675506 reads in 134.414 CPU sec, 4.261 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281716, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (361.81, 160.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675434 sequences (100000055 bp) in 3.053 CPU sec, 3.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281440, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (362.24, 161.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675510 sequences (100000186 bp) in 2.298 CPU sec, 2.305 real sec...
[M::mem_process_seqs] Processed 675548 reads in 197.494 CPU sec, 6.287 real sec
sorting block 162, #reads = 1074093 mem = 536879230 peak = 1131429888
[M::mem_process_seqs] Processed 675432 reads in 132.574 CPU sec, 4.196 real sec
sorting block 163, #reads = 1074476 mem = 536876182 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281468, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (362.51, 161.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675344 sequences (100000171 bp) in 3.051 CPU sec, 3.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281037, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (361.70, 161.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675434 reads in 200.740 CPU sec, 6.403 real sec
[M::process] read 675430 sequences (100000197 bp) in 2.504 CPU sec, 2.521 real sec...
[M::mem_process_seqs] Processed 675510 reads in 135.531 CPU sec, 4.279 real sec
sorting block 164, #reads = 1074543 mem = 536878077 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281764, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (364.53, 162.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675468 sequences (100000167 bp) in 3.773 CPU sec, 4.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281818, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (364.98, 163.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675344 reads in 193.544 CPU sec, 6.142 real sec
[M::mem_process_seqs] Processed 675430 reads in 126.176 CPU sec, 3.973 real sec
[M::process] read 680190 sequences (100000284 bp) in 2.594 CPU sec, 2.614 real sec...
sorting block 165, #reads = 1074225 mem = 536878299 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280831, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 347, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (364.49, 164.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683386 sequences (100000172 bp) in 2.711 CPU sec, 2.812 real sec...
[M::process] read 682710 sequences (100000123 bp) in 1.950 CPU sec, 1.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282703, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 315, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (335.86, 157.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675468 reads in 227.278 CPU sec, 7.228 real sec
sorting block 166, #reads = 1071855 mem = 536886014 peak = 1185476608
[M::mem_process_seqs] Processed 680190 reads in 158.994 CPU sec, 5.088 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283776, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 294, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (315.54, 148.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 167, #reads = 1064719 mem = 536879429 peak = 1185476608
[M::process] read 682680 sequences (100000134 bp) in 3.307 CPU sec, 3.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283997, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 298, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (318.31, 148.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681216 sequences (100000009 bp) in 2.593 CPU sec, 2.600 real sec...
[M::mem_process_seqs] Processed 683386 reads in 214.237 CPU sec, 6.805 real sec
[M::mem_process_seqs] Processed 682710 reads in 146.228 CPU sec, 4.636 real sec
sorting block 168, #reads = 1074116 mem = 536879777 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284254, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 300, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (319.28, 148.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680270 sequences (100000050 bp) in 3.173 CPU sec, 3.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283623, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (326.38, 150.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679886 sequences (100000111 bp) in 2.512 CPU sec, 2.520 real sec...
[M::mem_process_seqs] Processed 682680 reads in 221.233 CPU sec, 7.037 real sec
[M::mem_process_seqs] Processed 681216 reads in 149.353 CPU sec, 4.738 real sec
sorting block 169, #reads = 1072126 mem = 536879895 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283176, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 314, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (331.88, 152.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679296 sequences (100000179 bp) in 3.090 CPU sec, 3.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283153, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (334.28, 152.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680270 reads in 204.689 CPU sec, 6.505 real sec
[M::process] read 678472 sequences (100000258 bp) in 2.603 CPU sec, 2.616 real sec...
sorting block 170, #reads = 1073526 mem = 536880630 peak = 1185476608
[M::mem_process_seqs] Processed 679886 reads in 137.463 CPU sec, 4.331 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282873, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 320, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (337.20, 152.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678144 sequences (100000029 bp) in 3.200 CPU sec, 3.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282940, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (341.35, 154.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677946 sequences (100000300 bp) in 2.470 CPU sec, 2.475 real sec...
[M::mem_process_seqs] Processed 679296 reads in 195.726 CPU sec, 6.175 real sec
sorting block 171, #reads = 1073697 mem = 536878519 peak = 1185476608
[M::mem_process_seqs] Processed 678472 reads in 131.111 CPU sec, 4.135 real sec
sorting block 172, #reads = 1073661 mem = 536881187 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282186, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (345.85, 156.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677700 sequences (100000069 bp) in 3.422 CPU sec, 3.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283169, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (347.05, 156.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678144 reads in 194.571 CPU sec, 6.122 real sec
[M::process] read 677062 sequences (100000283 bp) in 3.158 CPU sec, 3.171 real sec...
[M::mem_process_seqs] Processed 677946 reads in 130.823 CPU sec, 4.105 real sec
sorting block 173, #reads = 1074434 mem = 536878874 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282584, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (349.07, 156.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676942 sequences (100000218 bp) in 2.863 CPU sec, 2.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282527, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 339, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (353.07, 156.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677000 sequences (100000085 bp) in 2.480 CPU sec, 2.477 real sec...
[M::mem_process_seqs] Processed 677700 reads in 192.241 CPU sec, 6.078 real sec
[M::mem_process_seqs] Processed 677062 reads in 130.213 CPU sec, 4.102 real sec
sorting block 174, #reads = 1075117 mem = 536878251 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282607, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (355.43, 158.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676724 sequences (100000263 bp) in 3.143 CPU sec, 3.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282381, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (356.35, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676626 sequences (100000135 bp) in 1.720 CPU sec, 1.718 real sec...
[M::mem_process_seqs] Processed 676942 reads in 165.927 CPU sec, 5.241 real sec
sorting block 175, #reads = 1074185 mem = 536878948 peak = 1185476608
[M::mem_process_seqs] Processed 677000 reads in 100.305 CPU sec, 3.165 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282574, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 343, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (357.67, 160.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676394 sequences (100000013 bp) in 2.638 CPU sec, 2.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281884, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 342, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (357.77, 160.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676724 reads in 163.325 CPU sec, 5.142 real sec
[M::process] read 676470 sequences (100000092 bp) in 2.378 CPU sec, 2.387 real sec...
sorting block 176, #reads = 1073845 mem = 536880694 peak = 1185476608
[M::mem_process_seqs] Processed 676626 reads in 115.691 CPU sec, 3.636 real sec
sorting block 177, #reads = 1073901 mem = 536878795 peak = 1185476608
[M::process] read 676218 sequences (100000223 bp) in 3.016 CPU sec, 3.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282002, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (355.51, 158.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676324 sequences (100000229 bp) in 2.410 CPU sec, 2.408 real sec...
[M::mem_process_seqs] Processed 676470 reads in 103.051 CPU sec, 3.231 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282187, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (357.13, 159.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281890, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (359.19, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676394 reads in 285.089 CPU sec, 8.982 real sec
[M::process] read 676340 sequences (100000241 bp) in 2.690 CPU sec, 2.697 real sec...
sorting block 178, #reads = 1074344 mem = 536879207 peak = 1185476608
[M::mem_process_seqs] Processed 676218 reads in 144.148 CPU sec, 4.560 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282216, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (358.62, 160.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 179, #reads = 1074634 mem = 536879815 peak = 1185476608
[M::process] read 676564 sequences (100000235 bp) in 2.687 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281215, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (357.92, 160.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675868 sequences (100000043 bp) in 1.780 CPU sec, 1.778 real sec...
[M::mem_process_seqs] Processed 676324 reads in 181.282 CPU sec, 5.756 real sec
[M::mem_process_seqs] Processed 676340 reads in 121.341 CPU sec, 3.844 real sec
sorting block 180, #reads = 1075625 mem = 536878090 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282054, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (356.92, 161.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675788 sequences (100000102 bp) in 3.084 CPU sec, 3.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282187, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 345, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (362.24, 162.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675752 sequences (100000134 bp) in 1.840 CPU sec, 1.839 real sec...
[M::mem_process_seqs] Processed 676564 reads in 180.455 CPU sec, 5.707 real sec
[M::mem_process_seqs] Processed 675868 reads in 121.200 CPU sec, 3.831 real sec
sorting block 181, #reads = 1074989 mem = 536879347 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281897, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (363.52, 163.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675696 sequences (100000301 bp) in 2.766 CPU sec, 2.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281567, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (360.60, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675788 reads in 175.024 CPU sec, 5.531 real sec
[M::process] read 675562 sequences (100000124 bp) in 2.359 CPU sec, 2.367 real sec...
sorting block 182, #reads = 1076152 mem = 536878455 peak = 1185476608
[M::mem_process_seqs] Processed 675752 reads in 116.607 CPU sec, 3.667 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281436, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 344, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (360.55, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675328 sequences (100000111 bp) in 3.013 CPU sec, 3.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281187, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (363.60, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675696 reads in 176.479 CPU sec, 5.559 real sec
[M::process] read 675260 sequences (100000098 bp) in 2.360 CPU sec, 2.363 real sec...
sorting block 183, #reads = 1076011 mem = 536877574 peak = 1185476608
[M::mem_process_seqs] Processed 675562 reads in 116.835 CPU sec, 3.680 real sec
sorting block 184, #reads = 1076937 mem = 536881908 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281636, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (365.22, 164.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675164 sequences (100000262 bp) in 2.567 CPU sec, 2.641 real sec...
[M::process] read 674834 sequences (100000228 bp) in 1.760 CPU sec, 1.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281349, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (366.12, 164.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675328 reads in 175.363 CPU sec, 5.558 real sec
[M::mem_process_seqs] Processed 675260 reads in 117.396 CPU sec, 3.718 real sec
sorting block 185, #reads = 1076971 mem = 536881017 peak = 1185476608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281993, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (368.61, 165.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675030 sequences (100000224 bp) in 2.928 CPU sec, 2.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281592, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (370.04, 167.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675164 reads in 173.977 CPU sec, 5.482 real sec
[M::process] read 675072 sequences (100000100 bp) in 2.420 CPU sec, 2.427 real sec...
[M::mem_process_seqs] Processed 674834 reads in 116.018 CPU sec, 3.656 real sec
sorting block 186, #reads = 1077932 mem = 536882018 peak = 1185476608
[M::process] read 124442 sequences (18402722 bp) in 0.548 CPU sec, 0.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280864, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 355, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (372.78, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281278, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 352, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (370.93, 168.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 51805, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (359.17, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675030 reads in 190.050 CPU sec, 6.138 real sec
sorting block 187, #reads = 1076490 mem = 536877914 peak = 1185476608
[M::mem_process_seqs] Processed 675072 reads in 136.017 CPU sec, 4.388 real sec
[M::mem_process_seqs] Processed 124442 reads in 140.915 CPU sec, 4.554 real sec
sorting block 188, #reads = 1075002 mem = 536882282 peak = 1214717952
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 202950320
overall: 69833981952 mem 29619.561 user 599.763 sys 1012.562 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_bR4-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tLB:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1012.600 sec; CPU: 30219.327 sec
sorting block 189, #reads = 44635 mem = 22478856 peak = 1214717952
uniq header count 1
merging 190 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 10994223714
output reads: 203791229
bam_mem_sort: 190 calls 122.368 user 2.005 sys 125.163 real
bam_write: 190 calls 79.003 user 4.229 sys 104.697 real
execute: 1 calls 60.497 user 1.664 sys 1096.670 real
merge_files: 1 calls 60.464 user 1.621 sys 84.001 real
parse_chunk: 3025 calls 182.676 user 39.123 sys 232.694 real
read_chunk: 8149 calls 85.300 user 56.522 sys 969.301 real
sort_block: 1 calls 60.564 user 56.161 sys 934.264 real
write_chunk: 5122 calls 60.077 user 1.217 sys 82.866 real
overall: 3344535552 mem 3361.232 user 127.857 sys 1096.692 real
ls: cannot access '*.cram': No such file or directory