This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_d0M-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1976.622 CPU sec, 68.585 real sec
[M::process] read 679716 sequences (100000158 bp) in 1.394 CPU sec, 1.397 real sec...
[M::process] read 678130 sequences (100000246 bp) in 1.675 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286084, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 300, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (324.67, 153.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677644 sequences (100000285 bp) in 1.618 CPU sec, 1.670 real sec...
[M::process] read 677464 sequences (100000242 bp) in 1.403 CPU sec, 1.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285503, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 315, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (337.04, 156.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679716 reads in 190.139 CPU sec, 6.140 real sec
[M::mem_process_seqs] Processed 678130 reads in 101.746 CPU sec, 3.326 real sec
sorting block 0, #reads = 1082905 mem = 536878597 peak = 606330880
[M::process] read 677154 sequences (100000102 bp) in 1.817 CPU sec, 2.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285771, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 318, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (339.37, 157.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676956 sequences (100000118 bp) in 1.608 CPU sec, 1.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285300, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 320, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (341.33, 157.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (940, 1384, 4411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11353)
[M::mem_pestat] mean and std.dev: (2266.09, 1736.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14824)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284782, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (342.03, 157.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677644 reads in 237.095 CPU sec, 7.659 real sec
[M::mem_process_seqs] Processed 677464 reads in 177.362 CPU sec, 5.778 real sec
[M::mem_process_seqs] Processed 677154 reads in 193.481 CPU sec, 6.332 real sec
sorting block 1, #reads = 1082438 mem = 536880620 peak = 733732864
[M::process] read 676404 sequences (100000235 bp) in 1.966 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284777, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (344.68, 158.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1080370 mem = 536878587 peak = 894820352
[M::process] read 676362 sequences (100000065 bp) in 2.303 CPU sec, 2.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284841, 1, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (347.68, 159.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 425, 860)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2184)
[M::mem_pestat] mean and std.dev: (464.64, 318.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2846)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676956 reads in 171.083 CPU sec, 5.485 real sec
[M::mem_process_seqs] Processed 676404 reads in 110.802 CPU sec, 3.506 real sec
[M::process] read 676272 sequences (100000123 bp) in 2.373 CPU sec, 2.524 real sec...
sorting block 3, #reads = 1080926 mem = 536879067 peak = 894820352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284816, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (350.40, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676058 sequences (100000082 bp) in 2.311 CPU sec, 2.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284888, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (351.21, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676362 reads in 165.086 CPU sec, 5.278 real sec
[M::process] read 676230 sequences (100000194 bp) in 1.701 CPU sec, 1.716 real sec...
[M::mem_process_seqs] Processed 676272 reads in 108.232 CPU sec, 3.447 real sec
sorting block 4, #reads = 1081599 mem = 536879020 peak = 894820352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285190, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (350.39, 160.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675874 sequences (100000103 bp) in 2.039 CPU sec, 2.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285075, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (353.37, 162.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675522 sequences (100000253 bp) in 1.784 CPU sec, 1.795 real sec...
[M::mem_process_seqs] Processed 676058 reads in 167.497 CPU sec, 5.415 real sec
[M::mem_process_seqs] Processed 676230 reads in 108.626 CPU sec, 3.486 real sec
sorting block 5, #reads = 1081868 mem = 536880168 peak = 894820352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284846, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 753, 1531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4319)
[M::mem_pestat] mean and std.dev: (700.20, 589.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (355.05, 161.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675642 sequences (100000134 bp) in 2.231 CPU sec, 2.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284859, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (357.14, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675562 sequences (100000042 bp) in 1.596 CPU sec, 1.691 real sec...
[M::mem_process_seqs] Processed 675874 reads in 167.657 CPU sec, 5.419 real sec
sorting block 6, #reads = 1080931 mem = 536881598 peak = 894820352
[M::mem_process_seqs] Processed 675522 reads in 109.298 CPU sec, 3.514 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285017, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (355.29, 161.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1080614 mem = 536878959 peak = 918286336
[M::process] read 675610 sequences (100000155 bp) in 1.876 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284990, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (356.74, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675332 sequences (100000122 bp) in 1.641 CPU sec, 1.876 real sec...
[M::mem_process_seqs] Processed 675642 reads in 170.027 CPU sec, 5.539 real sec
[M::mem_process_seqs] Processed 675562 reads in 110.442 CPU sec, 3.561 real sec
sorting block 8, #reads = 1082267 mem = 536878593 peak = 918286336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284636, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (354.18, 161.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675250 sequences (100000231 bp) in 2.313 CPU sec, 2.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284881, 12, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (352.03, 159.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1068, 1098, 4418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11118)
[M::mem_pestat] mean and std.dev: (1754.67, 1579.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14468)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675436 sequences (100000010 bp) in 1.927 CPU sec, 1.965 real sec...
[M::mem_process_seqs] Processed 675610 reads in 168.421 CPU sec, 5.476 real sec
[M::mem_process_seqs] Processed 675332 reads in 107.312 CPU sec, 3.433 real sec
sorting block 9, #reads = 1080365 mem = 536877781 peak = 918286336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284251, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (301, 334, 1374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3520)
[M::mem_pestat] mean and std.dev: (666.90, 490.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (351.01, 158.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675700 sequences (100000005 bp) in 2.304 CPU sec, 2.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284261, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (348.54, 157.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 201, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (74, 384)
[M::mem_pestat] mean and std.dev: (232.36, 67.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 502)
[M::mem_pestat] skip orientation RR
[M::process] read 675634 sequences (100000184 bp) in 1.842 CPU sec, 1.851 real sec...
[M::mem_process_seqs] Processed 675250 reads in 165.839 CPU sec, 5.308 real sec
[M::mem_process_seqs] Processed 675436 reads in 106.073 CPU sec, 3.376 real sec
sorting block 10, #reads = 1080797 mem = 536880736 peak = 918286336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284088, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 326, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (345.46, 155.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675636 sequences (100000223 bp) in 2.590 CPU sec, 2.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284852, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (319, 348, 660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1342)
[M::mem_pestat] mean and std.dev: (465.92, 164.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1683)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 326, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (346.15, 156.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675774 sequences (100000288 bp) in 1.846 CPU sec, 1.849 real sec...
[M::mem_process_seqs] Processed 675700 reads in 167.941 CPU sec, 5.379 real sec
sorting block 11, #reads = 1080353 mem = 536880058 peak = 918286336
[M::mem_process_seqs] Processed 675634 reads in 109.481 CPU sec, 3.477 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285060, 5, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (581, 581, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (581, 581)
[M::mem_pestat] mean and std.dev: (581.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (581, 581)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 325, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (344.61, 154.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 12, #reads = 1080799 mem = 536879407 peak = 950358016
[M::process] read 675776 sequences (100000298 bp) in 2.238 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284609, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 325, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (344.39, 155.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675636 reads in 166.693 CPU sec, 5.418 real sec
[M::mem_process_seqs] Processed 675774 reads in 108.896 CPU sec, 3.506 real sec
[M::process] read 675668 sequences (100000195 bp) in 1.974 CPU sec, 2.058 real sec...
sorting block 13, #reads = 1080381 mem = 536883891 peak = 950358016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284450, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 324, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (343.97, 154.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675554 sequences (100000273 bp) in 2.270 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284672, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 326, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (345.33, 155.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675776 reads in 164.374 CPU sec, 5.326 real sec
[M::mem_process_seqs] Processed 675668 reads in 109.132 CPU sec, 3.490 real sec
[M::process] read 675544 sequences (100000012 bp) in 1.835 CPU sec, 2.033 real sec...
sorting block 14, #reads = 1079767 mem = 536878409 peak = 950358016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284206, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 328, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (347.34, 156.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 220, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (139, 274)
[M::mem_pestat] mean and std.dev: (215.10, 21.39)
[M::mem_pestat] low and high boundaries for proper pairs: (112, 301)
[M::mem_pestat] skip orientation RR
[M::process] read 675160 sequences (100000206 bp) in 2.343 CPU sec, 2.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284971, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (349.14, 157.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675554 reads in 167.264 CPU sec, 5.459 real sec
[M::mem_process_seqs] Processed 675544 reads in 109.462 CPU sec, 3.542 real sec
sorting block 15, #reads = 1079752 mem = 536882241 peak = 950358016
[M::process] read 675040 sequences (100000271 bp) in 2.168 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284403, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (353.02, 159.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678080 sequences (100000180 bp) in 2.372 CPU sec, 2.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284652, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 495, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (362.92, 146.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (351.62, 157.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675160 reads in 166.422 CPU sec, 5.436 real sec
sorting block 16, #reads = 1078453 mem = 536880610 peak = 971116544
[M::process] read 677936 sequences (100000185 bp) in 1.884 CPU sec, 1.930 real sec...
[M::mem_process_seqs] Processed 675040 reads in 110.594 CPU sec, 3.620 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284966, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 314, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (337.30, 157.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1079405 mem = 536878195 peak = 973905920
[M::process] read 677646 sequences (100000009 bp) in 2.345 CPU sec, 2.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285279, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 315, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (337.77, 157.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678080 reads in 165.696 CPU sec, 5.461 real sec
[M::mem_process_seqs] Processed 677936 reads in 107.652 CPU sec, 3.527 real sec
[M::process] read 677442 sequences (100000044 bp) in 1.940 CPU sec, 2.065 real sec...
sorting block 18, #reads = 1081090 mem = 536877839 peak = 973905920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285075, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 318, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (339.17, 156.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677236 sequences (100000291 bp) in 2.464 CPU sec, 2.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285635, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (341.68, 157.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677646 reads in 160.942 CPU sec, 5.210 real sec
[M::mem_process_seqs] Processed 677442 reads in 105.999 CPU sec, 3.395 real sec
[M::process] read 677036 sequences (100000125 bp) in 2.247 CPU sec, 2.377 real sec...
sorting block 19, #reads = 1083142 mem = 536880892 peak = 973905920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284670, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 475, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125)
[M::mem_pestat] mean and std.dev: (273.90, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1450)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (343.81, 158.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676598 sequences (100000220 bp) in 2.281 CPU sec, 2.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285182, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (345.91, 158.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677236 reads in 162.203 CPU sec, 5.272 real sec
[M::mem_process_seqs] Processed 677036 reads in 107.911 CPU sec, 3.449 real sec
sorting block 20, #reads = 1082772 mem = 536877873 peak = 973905920
[M::process] read 676238 sequences (100000295 bp) in 2.121 CPU sec, 2.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285504, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (348.28, 159.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676176 sequences (100000240 bp) in 2.355 CPU sec, 2.654 real sec...
sorting block 21, #reads = 1080930 mem = 536882310 peak = 983093248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284745, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (350.41, 160.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676598 reads in 162.516 CPU sec, 5.247 real sec
[M::mem_process_seqs] Processed 676238 reads in 106.351 CPU sec, 3.437 real sec
[M::process] read 675944 sequences (100000190 bp) in 1.921 CPU sec, 2.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284286, 2, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (402, 1619, 1619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4053)
[M::mem_pestat] mean and std.dev: (1180.23, 998.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5270)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (351.65, 160.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 22, #reads = 1082301 mem = 536879685 peak = 983093248
[M::process] read 675964 sequences (100000289 bp) in 2.327 CPU sec, 2.749 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284624, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (352.75, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676176 reads in 162.321 CPU sec, 5.266 real sec
[M::mem_process_seqs] Processed 675944 reads in 107.655 CPU sec, 3.437 real sec
[M::process] read 675786 sequences (100000176 bp) in 2.007 CPU sec, 2.068 real sec...
sorting block 23, #reads = 1081924 mem = 536878697 peak = 983093248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284709, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.31, 159.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675840 sequences (100000155 bp) in 2.521 CPU sec, 2.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284586, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (354.42, 161.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675964 reads in 155.976 CPU sec, 5.058 real sec
[M::mem_process_seqs] Processed 675786 reads in 105.878 CPU sec, 3.398 real sec
[M::process] read 675556 sequences (100000208 bp) in 2.100 CPU sec, 2.195 real sec...
sorting block 24, #reads = 1082582 mem = 536879980 peak = 983093248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284600, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (356.13, 162.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675454 sequences (100000187 bp) in 2.038 CPU sec, 2.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284122, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (356.09, 162.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675840 reads in 161.459 CPU sec, 5.250 real sec
[M::mem_process_seqs] Processed 675556 reads in 107.012 CPU sec, 3.452 real sec
[M::process] read 675186 sequences (100000225 bp) in 2.089 CPU sec, 2.243 real sec...
sorting block 25, #reads = 1081651 mem = 536878721 peak = 983093248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284425, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (356.13, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675228 sequences (100000097 bp) in 2.573 CPU sec, 2.827 real sec...
sorting block 26, #reads = 1080519 mem = 536879835 peak = 983093248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284831, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (358.98, 163.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675454 reads in 161.117 CPU sec, 5.208 real sec
[M::process] read 675432 sequences (100000090 bp) in 2.025 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284472, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (352.74, 158.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675228 reads in 93.673 CPU sec, 3.005 real sec
[M::process] read 675482 sequences (100000131 bp) in 1.904 CPU sec, 1.970 real sec...
sorting block 27, #reads = 1080864 mem = 536883655 peak = 983093248
[M::mem_process_seqs] Processed 675186 reads in 236.512 CPU sec, 7.625 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284563, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (374, 1036, 2903)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7961)
[M::mem_pestat] mean and std.dev: (1635.82, 1710.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 328, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (346.36, 154.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675290 sequences (100000029 bp) in 2.708 CPU sec, 3.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284750, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 328, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (347.76, 156.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675432 reads in 185.175 CPU sec, 5.974 real sec
sorting block 28, #reads = 1080108 mem = 536879415 peak = 983093248
[M::mem_process_seqs] Processed 675482 reads in 109.869 CPU sec, 3.523 real sec
[M::process] read 675556 sequences (100000105 bp) in 2.506 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284303, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (348.59, 156.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1080499 mem = 536883075 peak = 983093248
[M::process] read 675616 sequences (100000219 bp) in 2.391 CPU sec, 2.807 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284829, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 326, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (345.64, 155.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675290 reads in 171.958 CPU sec, 5.600 real sec
[M::mem_process_seqs] Processed 675556 reads in 109.810 CPU sec, 3.543 real sec
[M::process] read 675522 sequences (100000078 bp) in 2.313 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284452, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (347.16, 156.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 30, #reads = 1080031 mem = 536880720 peak = 983093248
[M::process] read 675374 sequences (100000023 bp) in 2.629 CPU sec, 2.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284780, 12, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (347.61, 156.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (413, 1203, 1476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3602)
[M::mem_pestat] mean and std.dev: (925.42, 552.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4665)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675616 reads in 166.926 CPU sec, 5.392 real sec
[M::mem_process_seqs] Processed 675522 reads in 108.860 CPU sec, 3.490 real sec
[M::process] read 675752 sequences (100000260 bp) in 2.154 CPU sec, 2.334 real sec...
sorting block 31, #reads = 1080157 mem = 536878687 peak = 983093248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284774, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (347.97, 156.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675702 sequences (100000151 bp) in 2.514 CPU sec, 3.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284809, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 328, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (346.94, 156.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675374 reads in 170.046 CPU sec, 5.620 real sec
[M::mem_process_seqs] Processed 675752 reads in 109.942 CPU sec, 3.669 real sec
[M::process] read 675490 sequences (100000199 bp) in 2.057 CPU sec, 2.193 real sec...
sorting block 32, #reads = 1079802 mem = 536878458 peak = 983093248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284796, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 328, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (346.96, 156.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675480 sequences (100000060 bp) in 2.615 CPU sec, 2.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284871, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (349.41, 158.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 286, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (264.93, 72.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675702 reads in 168.987 CPU sec, 5.567 real sec
[M::mem_process_seqs] Processed 675490 reads in 111.309 CPU sec, 3.709 real sec
sorting block 33, #reads = 1080234 mem = 536879718 peak = 983093248
[M::process] read 675382 sequences (100000158 bp) in 2.254 CPU sec, 2.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285088, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (352.41, 159.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (486, 606, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (246, 846)
[M::mem_pestat] mean and std.dev: (591.00, 39.69)
[M::mem_pestat] low and high boundaries for proper pairs: (126, 966)
[M::mem_pestat] skip orientation RR
sorting block 34, #reads = 1079750 mem = 536880023 peak = 1003024384
[M::process] read 677066 sequences (100000279 bp) in 2.775 CPU sec, 3.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284820, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (354.71, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675480 reads in 172.241 CPU sec, 5.665 real sec
[M::mem_process_seqs] Processed 675382 reads in 115.570 CPU sec, 3.850 real sec
[M::process] read 678442 sequences (100000189 bp) in 2.092 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285428, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (308, 625, 818)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1838)
[M::mem_pestat] mean and std.dev: (523.30, 241.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2348)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (342.65, 158.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (357, 524, 828)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1770)
[M::mem_pestat] mean and std.dev: (490.62, 229.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2241)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 35, #reads = 1079473 mem = 536878465 peak = 1003024384
[M::process] read 678590 sequences (100000215 bp) in 2.498 CPU sec, 2.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285318, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 311, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (334.19, 156.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677066 reads in 173.076 CPU sec, 5.636 real sec
[M::mem_process_seqs] Processed 678442 reads in 112.462 CPU sec, 3.589 real sec
[M::process] read 678146 sequences (100000113 bp) in 2.401 CPU sec, 2.501 real sec...
sorting block 36, #reads = 1079931 mem = 536880266 peak = 1003024384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285328, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (334.35, 156.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678128 sequences (100000271 bp) in 2.352 CPU sec, 3.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285626, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 314, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (335.77, 155.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678590 reads in 167.033 CPU sec, 5.485 real sec
[M::mem_process_seqs] Processed 678146 reads in 108.147 CPU sec, 3.535 real sec
sorting block 37, #reads = 1082044 mem = 536879940 peak = 1003024384
[M::process] read 677924 sequences (100000119 bp) in 2.395 CPU sec, 2.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285307, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (337.90, 157.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677760 sequences (100000294 bp) in 2.432 CPU sec, 2.850 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285646, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (338.77, 157.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678128 reads in 167.488 CPU sec, 5.456 real sec
sorting block 38, #reads = 1082955 mem = 536879359 peak = 1003024384
[M::mem_process_seqs] Processed 677924 reads in 108.882 CPU sec, 3.528 real sec
[M::process] read 677064 sequences (100000227 bp) in 2.393 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285389, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (342.30, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1082366 mem = 536877618 peak = 1003024384
[M::process] read 677064 sequences (100000038 bp) in 2.543 CPU sec, 3.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285805, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (345.97, 159.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677760 reads in 168.664 CPU sec, 5.542 real sec
[M::mem_process_seqs] Processed 677064 reads in 106.929 CPU sec, 3.485 real sec
[M::process] read 676874 sequences (100000284 bp) in 2.281 CPU sec, 2.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284932, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (345.23, 158.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1081996 mem = 536878609 peak = 1003024384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285250, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (348.17, 160.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676624 sequences (100000233 bp) in 2.458 CPU sec, 3.000 real sec...
[M::mem_process_seqs] Processed 677064 reads in 170.344 CPU sec, 5.657 real sec
[M::mem_process_seqs] Processed 676874 reads in 107.752 CPU sec, 3.586 real sec
[M::process] read 676194 sequences (100000267 bp) in 2.464 CPU sec, 2.725 real sec...
sorting block 41, #reads = 1081454 mem = 536879221 peak = 1003024384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285574, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 669, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541)
[M::mem_pestat] mean and std.dev: (521.60, 226.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1977)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.68, 160.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676624 reads in 94.690 CPU sec, 3.299 real sec
[M::process] read 676292 sequences (100000163 bp) in 2.345 CPU sec, 2.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284404, 11, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 835, 2531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7043)
[M::mem_pestat] mean and std.dev: (1269.40, 1054.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9299)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (351.40, 160.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (766, 4104, 9504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 26980)
[M::mem_pestat] mean and std.dev: (4864.73, 4362.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 35718)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676194 reads in 90.735 CPU sec, 3.160 real sec
sorting block 42, #reads = 1081418 mem = 536880080 peak = 1003024384
[M::process] read 676148 sequences (100000066 bp) in 2.445 CPU sec, 2.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284701, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 359, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (275.27, 92.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (354.05, 163.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (403, 403, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (403, 403)
[M::mem_pestat] mean and std.dev: (403.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (403, 403)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 676236 sequences (100000079 bp) in 2.515 CPU sec, 2.829 real sec...
sorting block 43, #reads = 1081648 mem = 536879659 peak = 1003024384
[M::mem_process_seqs] Processed 676148 reads in 95.435 CPU sec, 3.326 real sec
[M::process] read 675872 sequences (100000221 bp) in 2.477 CPU sec, 2.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284645, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (353.74, 162.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284930, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 336, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (355.39, 163.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676236 reads in 93.763 CPU sec, 3.187 real sec
[M::mem_process_seqs] Processed 676292 reads in 288.048 CPU sec, 9.836 real sec
sorting block 44, #reads = 1081111 mem = 536881523 peak = 1003024384
[M::process] read 676062 sequences (100000004 bp) in 2.314 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284937, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (354.70, 162.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676466 sequences (100000236 bp) in 2.532 CPU sec, 3.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284923, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (350.06, 160.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675872 reads in 172.043 CPU sec, 5.616 real sec
[M::mem_process_seqs] Processed 676062 reads in 110.666 CPU sec, 3.595 real sec
sorting block 45, #reads = 1082168 mem = 536879372 peak = 1003024384
[M::process] read 676346 sequences (100000213 bp) in 2.320 CPU sec, 2.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285398, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 320, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (341.09, 155.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1081175 mem = 536882609 peak = 1003024384
[M::process] read 675880 sequences (100000060 bp) in 2.462 CPU sec, 2.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285446, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 324, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (344.52, 157.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676466 reads in 168.733 CPU sec, 5.559 real sec
[M::mem_process_seqs] Processed 676346 reads in 111.757 CPU sec, 3.622 real sec
[M::process] read 675350 sequences (100000079 bp) in 2.327 CPU sec, 2.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284887, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.16, 160.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1081437 mem = 536879904 peak = 1003024384
[M::process] read 675544 sequences (100000119 bp) in 2.359 CPU sec, 3.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284811, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (354.82, 160.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675880 reads in 169.440 CPU sec, 5.557 real sec
[M::mem_process_seqs] Processed 675350 reads in 110.406 CPU sec, 3.592 real sec
[M::process] read 675536 sequences (100000263 bp) in 2.481 CPU sec, 2.628 real sec...
sorting block 48, #reads = 1081161 mem = 536881530 peak = 1003024384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284309, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (352.71, 160.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675272 sequences (100000172 bp) in 2.586 CPU sec, 3.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284521, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (353.35, 160.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675544 reads in 170.236 CPU sec, 5.534 real sec
[M::mem_process_seqs] Processed 675536 reads in 111.364 CPU sec, 3.654 real sec
sorting block 49, #reads = 1081267 mem = 536884138 peak = 1003024384
[M::process] read 675418 sequences (100000019 bp) in 2.116 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284324, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (353.36, 160.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675632 sequences (100000255 bp) in 2.532 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284953, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (353.25, 161.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675272 reads in 171.560 CPU sec, 5.655 real sec
[M::mem_process_seqs] Processed 675418 reads in 111.831 CPU sec, 3.643 real sec
sorting block 50, #reads = 1080762 mem = 536882938 peak = 1003024384
[M::process] read 675634 sequences (100000112 bp) in 2.639 CPU sec, 2.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284647, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.26, 159.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1079031 mem = 536878483 peak = 1003024384
[M::process] read 675766 sequences (100000108 bp) in 2.511 CPU sec, 2.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284651, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (351.50, 159.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675632 reads in 170.524 CPU sec, 5.676 real sec
[M::mem_process_seqs] Processed 675634 reads in 113.321 CPU sec, 3.864 real sec
[M::process] read 675038 sequences (100000012 bp) in 2.022 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285206, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (352.34, 161.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 52, #reads = 1079402 mem = 536877561 peak = 1003024384
[M::process] read 676700 sequences (100000274 bp) in 2.555 CPU sec, 2.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284819, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (359.65, 164.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (670, 670, 1180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2200)
[M::mem_pestat] mean and std.dev: (716.27, 351.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2710)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675766 reads in 168.407 CPU sec, 5.520 real sec
[M::mem_process_seqs] Processed 675038 reads in 110.450 CPU sec, 3.640 real sec
[M::process] read 678552 sequences (100000044 bp) in 2.258 CPU sec, 2.446 real sec...
sorting block 53, #reads = 1078639 mem = 536879568 peak = 1003024384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284712, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (347.21, 161.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678220 sequences (100000090 bp) in 2.563 CPU sec, 3.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285119, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 311, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (334.50, 156.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676700 reads in 172.661 CPU sec, 5.670 real sec
[M::mem_process_seqs] Processed 678552 reads in 108.438 CPU sec, 3.529 real sec
sorting block 54, #reads = 1078710 mem = 536879887 peak = 1003024384
[M::process] read 678004 sequences (100000167 bp) in 2.072 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285073, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (335.67, 156.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677978 sequences (100000213 bp) in 2.590 CPU sec, 3.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285007, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 313, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (335.31, 155.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678220 reads in 168.746 CPU sec, 5.499 real sec
sorting block 55, #reads = 1083333 mem = 536879873 peak = 1003024384
[M::mem_process_seqs] Processed 678004 reads in 109.781 CPU sec, 3.529 real sec
[M::process] read 677624 sequences (100000008 bp) in 2.380 CPU sec, 2.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285312, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 315, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (337.18, 156.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1083226 mem = 536883290 peak = 1034010624
[M::process] read 678034 sequences (100000294 bp) in 2.371 CPU sec, 2.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285305, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 318, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (339.81, 158.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (312, 338, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (260, 390)
[M::mem_pestat] mean and std.dev: (329.33, 12.26)
[M::mem_pestat] low and high boundaries for proper pairs: (234, 416)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677978 reads in 167.382 CPU sec, 5.443 real sec
[M::mem_process_seqs] Processed 677624 reads in 108.906 CPU sec, 3.508 real sec
[M::process] read 677502 sequences (100000025 bp) in 2.171 CPU sec, 2.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285190, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (339.31, 159.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 57, #reads = 1083363 mem = 536878310 peak = 1034010624
[M::process] read 677282 sequences (100000190 bp) in 2.591 CPU sec, 2.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285101, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 322, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (342.83, 158.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678034 reads in 165.077 CPU sec, 5.347 real sec
[M::mem_process_seqs] Processed 677502 reads in 109.446 CPU sec, 3.518 real sec
[M::process] read 677250 sequences (100000098 bp) in 2.426 CPU sec, 2.639 real sec...
sorting block 58, #reads = 1083895 mem = 536880980 peak = 1034010624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284830, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (343.92, 159.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676874 sequences (100000132 bp) in 2.508 CPU sec, 2.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284117, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (343.77, 159.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677282 reads in 166.884 CPU sec, 5.423 real sec
[M::mem_process_seqs] Processed 677250 reads in 109.133 CPU sec, 3.514 real sec
sorting block 59, #reads = 1082583 mem = 536879034 peak = 1034010624
[M::process] read 676760 sequences (100000129 bp) in 2.280 CPU sec, 2.413 real sec...
[M::process] read 676530 sequences (100000060 bp) in 2.545 CPU sec, 2.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284857, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (349.37, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676760 reads in 93.902 CPU sec, 3.008 real sec
sorting block 60, #reads = 1082001 mem = 536878501 peak = 1034010624
[M::process] read 676558 sequences (100000028 bp) in 2.444 CPU sec, 2.672 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284954, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (346.94, 160.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283938, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.72, 161.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676874 reads in 259.759 CPU sec, 8.403 real sec
[M::mem_process_seqs] Processed 676530 reads in 125.887 CPU sec, 4.036 real sec
[M::process] read 676544 sequences (100000099 bp) in 2.120 CPU sec, 2.377 real sec...
sorting block 61, #reads = 1082416 mem = 536877094 peak = 1034010624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284771, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (353.17, 163.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676008 sequences (100000100 bp) in 2.661 CPU sec, 3.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284672, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (352.33, 162.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676558 reads in 168.499 CPU sec, 5.530 real sec
[M::mem_process_seqs] Processed 676544 reads in 109.881 CPU sec, 3.536 real sec
sorting block 62, #reads = 1081776 mem = 536879338 peak = 1034010624
[M::process] read 676194 sequences (100000216 bp) in 2.181 CPU sec, 2.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284501, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.67, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1221, 1221, 5476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13986)
[M::mem_pestat] mean and std.dev: (2359.85, 2112.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18241)
[M::mem_pestat] skip orientation RR
sorting block 63, #reads = 1082299 mem = 536878478 peak = 1039437824
[M::process] read 676010 sequences (100000078 bp) in 2.701 CPU sec, 3.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284821, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 246, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 498)
[M::mem_pestat] mean and std.dev: (231.00, 57.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 582)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (353.23, 162.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676008 reads in 168.981 CPU sec, 5.476 real sec
[M::mem_process_seqs] Processed 676194 reads in 108.988 CPU sec, 3.584 real sec
[M::process] read 675672 sequences (100000109 bp) in 1.823 CPU sec, 1.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284661, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (353.30, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 64, #reads = 1082347 mem = 536877985 peak = 1039437824
[M::process] read 675574 sequences (100000183 bp) in 2.736 CPU sec, 3.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284356, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.74, 159.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676010 reads in 169.453 CPU sec, 5.593 real sec
[M::mem_process_seqs] Processed 675672 reads in 109.810 CPU sec, 3.641 real sec
[M::process] read 675334 sequences (100000191 bp) in 2.605 CPU sec, 2.799 real sec...
sorting block 65, #reads = 1081357 mem = 536876396 peak = 1039437824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285433, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (353.09, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675266 sequences (100000267 bp) in 2.355 CPU sec, 2.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284686, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 267, 2164)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5972)
[M::mem_pestat] mean and std.dev: (772.55, 856.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7876)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (355.03, 159.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675574 reads in 168.467 CPU sec, 5.523 real sec
[M::mem_process_seqs] Processed 675334 reads in 107.657 CPU sec, 3.520 real sec
sorting block 66, #reads = 1080638 mem = 536877692 peak = 1039437824
[M::process] read 675208 sequences (100000237 bp) in 2.362 CPU sec, 2.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284266, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (355.37, 160.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675182 sequences (100000112 bp) in 2.447 CPU sec, 2.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284514, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (354.32, 158.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675266 reads in 169.272 CPU sec, 5.544 real sec
[M::mem_process_seqs] Processed 675208 reads in 110.730 CPU sec, 3.617 real sec
sorting block 67, #reads = 1080401 mem = 536880248 peak = 1039437824
[M::process] read 675040 sequences (100000272 bp) in 2.529 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284318, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (353.79, 158.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (325, 408, 8154)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23812)
[M::mem_pestat] mean and std.dev: (3133.00, 3796.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 31641)
[M::mem_pestat] skip orientation RR
sorting block 68, #reads = 1080033 mem = 536879441 peak = 1039437824
[M::process] read 675092 sequences (100000239 bp) in 2.575 CPU sec, 2.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285115, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (355.89, 159.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675182 reads in 169.938 CPU sec, 5.571 real sec
[M::mem_process_seqs] Processed 675040 reads in 112.625 CPU sec, 3.733 real sec
[M::process] read 675222 sequences (100000220 bp) in 2.085 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284649, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (353.33, 158.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1079857 mem = 536879473 peak = 1039437824
[M::process] read 675168 sequences (100000299 bp) in 2.403 CPU sec, 2.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284412, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 266, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (220.75, 80.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (351.16, 157.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675092 reads in 169.129 CPU sec, 5.527 real sec
[M::mem_process_seqs] Processed 675222 reads in 111.673 CPU sec, 3.635 real sec
[M::process] read 675498 sequences (100000182 bp) in 2.427 CPU sec, 2.769 real sec...
sorting block 70, #reads = 1079968 mem = 536880184 peak = 1039437824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284739, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (350.57, 156.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675108 sequences (100000212 bp) in 2.626 CPU sec, 2.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284832, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (350.50, 157.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675168 reads in 168.384 CPU sec, 5.456 real sec
[M::mem_process_seqs] Processed 675498 reads in 111.160 CPU sec, 3.598 real sec
sorting block 71, #reads = 1078540 mem = 536877120 peak = 1039437824
[M::process] read 680020 sequences (100000056 bp) in 2.367 CPU sec, 2.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284582, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (354.72, 159.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680300 sequences (100000293 bp) in 2.739 CPU sec, 2.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286780, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 297, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (322.91, 153.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675108 reads in 179.118 CPU sec, 5.736 real sec
sorting block 72, #reads = 1078779 mem = 536879514 peak = 1078202368
[M::mem_process_seqs] Processed 680020 reads in 121.744 CPU sec, 3.861 real sec
[M::process] read 679928 sequences (100000057 bp) in 2.055 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287065, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 298, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (326.74, 157.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 73, #reads = 1077303 mem = 536884387 peak = 1107369984
[M::process] read 679762 sequences (100000264 bp) in 2.822 CPU sec, 3.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286718, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 301, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (328.89, 157.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678510 sequences (100000037 bp) in 1.841 CPU sec, 1.843 real sec...
[M::mem_process_seqs] Processed 680300 reads in 181.051 CPU sec, 5.851 real sec
[M::mem_process_seqs] Processed 679928 reads in 119.924 CPU sec, 3.864 real sec
sorting block 74, #reads = 1079620 mem = 536878004 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286303, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 302, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (328.62, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677936 sequences (100000122 bp) in 2.732 CPU sec, 3.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286050, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (337.10, 158.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677840 sequences (100000165 bp) in 2.004 CPU sec, 2.015 real sec...
[M::mem_process_seqs] Processed 679762 reads in 179.355 CPU sec, 5.731 real sec
sorting block 75, #reads = 1078888 mem = 536880318 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286398, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 318, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (340.90, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677936 reads in 93.689 CPU sec, 2.985 real sec
[M::process] read 677364 sequences (100000123 bp) in 2.300 CPU sec, 2.401 real sec...
[M::mem_process_seqs] Processed 678510 reads in 244.104 CPU sec, 7.796 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285769, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (343.19, 160.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 76, #reads = 1081071 mem = 536877388 peak = 1107369984
[M::process] read 677060 sequences (100000133 bp) in 2.829 CPU sec, 3.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285545, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 325, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (347.17, 162.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677840 reads in 190.209 CPU sec, 6.086 real sec
[M::mem_process_seqs] Processed 677364 reads in 111.165 CPU sec, 3.524 real sec
[M::process] read 677072 sequences (100000001 bp) in 2.077 CPU sec, 2.131 real sec...
sorting block 77, #reads = 1080149 mem = 536877683 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285804, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 302, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 495)
[M::mem_pestat] mean and std.dev: (264.64, 57.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (349.48, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676764 sequences (100000033 bp) in 2.458 CPU sec, 2.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285623, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (347.66, 160.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677060 reads in 173.278 CPU sec, 5.577 real sec
[M::process] read 676506 sequences (100000100 bp) in 2.045 CPU sec, 2.082 real sec...
[M::mem_process_seqs] Processed 677072 reads in 113.795 CPU sec, 3.618 real sec
sorting block 78, #reads = 1080635 mem = 536878323 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285599, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (352.86, 163.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676580 sequences (100000195 bp) in 2.799 CPU sec, 3.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285685, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (355.99, 165.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676764 reads in 178.111 CPU sec, 5.747 real sec
[M::process] read 676222 sequences (100000010 bp) in 1.843 CPU sec, 1.853 real sec...
sorting block 79, #reads = 1079035 mem = 536881866 peak = 1107369984
[M::mem_process_seqs] Processed 676506 reads in 116.179 CPU sec, 3.696 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285986, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (355.01, 165.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1078736 mem = 536881930 peak = 1107369984
[M::process] read 676070 sequences (100000058 bp) in 2.923 CPU sec, 3.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285165, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (354.47, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676580 reads in 179.360 CPU sec, 5.754 real sec
[M::process] read 676194 sequences (100000207 bp) in 1.991 CPU sec, 2.011 real sec...
[M::mem_process_seqs] Processed 676222 reads in 118.225 CPU sec, 3.742 real sec
sorting block 81, #reads = 1079479 mem = 536878881 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285030, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (352.71, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (459, 1355, 1861)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4665)
[M::mem_pestat] mean and std.dev: (1071.64, 789.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6067)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675632 sequences (100000015 bp) in 2.870 CPU sec, 3.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284933, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.76, 161.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675660 sequences (100000209 bp) in 1.784 CPU sec, 1.796 real sec...
[M::mem_process_seqs] Processed 676070 reads in 185.347 CPU sec, 5.943 real sec
[M::mem_process_seqs] Processed 676194 reads in 120.974 CPU sec, 3.850 real sec
sorting block 82, #reads = 1077064 mem = 536880025 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285032, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.11, 161.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675886 sequences (100000047 bp) in 2.934 CPU sec, 3.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284909, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (352.07, 160.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675632 reads in 182.026 CPU sec, 5.848 real sec
[M::mem_process_seqs] Processed 675660 reads in 120.148 CPU sec, 3.826 real sec
[M::process] read 676026 sequences (100000017 bp) in 2.022 CPU sec, 2.087 real sec...
sorting block 83, #reads = 1078081 mem = 536887930 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284828, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (352.82, 161.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676102 sequences (100000220 bp) in 2.875 CPU sec, 3.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284295, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (350.45, 160.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675886 reads in 184.990 CPU sec, 5.936 real sec
[M::process] read 676546 sequences (100000059 bp) in 2.028 CPU sec, 2.034 real sec...
sorting block 84, #reads = 1077861 mem = 536880967 peak = 1107369984
[M::mem_process_seqs] Processed 676026 reads in 124.602 CPU sec, 3.970 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285229, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (349.86, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 181, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (216.92, 60.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] skip orientation RR
sorting block 85, #reads = 1077615 mem = 536880874 peak = 1107369984
[M::process] read 676094 sequences (100000186 bp) in 2.898 CPU sec, 3.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285694, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (347.73, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676102 reads in 184.611 CPU sec, 5.934 real sec
[M::process] read 676124 sequences (100000226 bp) in 2.234 CPU sec, 2.310 real sec...
[M::mem_process_seqs] Processed 676546 reads in 123.617 CPU sec, 3.932 real sec
sorting block 86, #reads = 1077326 mem = 536881125 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285182, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.16, 160.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (939, 6809, 6809)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18549)
[M::mem_pestat] mean and std.dev: (5043.00, 2697.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 24419)
[M::mem_pestat] skip orientation RR
[M::process] read 675896 sequences (100000053 bp) in 2.809 CPU sec, 3.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285146, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (350.92, 161.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 140, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (249.58, 193.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RR
[M::process] read 675914 sequences (100000001 bp) in 2.004 CPU sec, 2.017 real sec...
[M::mem_process_seqs] Processed 676094 reads in 184.912 CPU sec, 5.909 real sec
[M::mem_process_seqs] Processed 676124 reads in 122.270 CPU sec, 3.892 real sec
sorting block 87, #reads = 1077783 mem = 536878232 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284816, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (349.81, 159.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676012 sequences (100000079 bp) in 2.868 CPU sec, 3.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284526, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (350.00, 160.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (407, 407, 1131)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579)
[M::mem_pestat] mean and std.dev: (640.75, 591.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3303)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675896 reads in 182.453 CPU sec, 5.856 real sec
[M::process] read 679656 sequences (100000153 bp) in 2.134 CPU sec, 2.151 real sec...
[M::mem_process_seqs] Processed 675914 reads in 120.645 CPU sec, 3.844 real sec
sorting block 88, #reads = 1077475 mem = 536878094 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285422, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.85, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681634 sequences (100000039 bp) in 2.880 CPU sec, 3.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286455, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 302, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (327.29, 153.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676012 reads in 192.659 CPU sec, 6.210 real sec
[M::process] read 680818 sequences (100000059 bp) in 1.892 CPU sec, 1.931 real sec...
sorting block 89, #reads = 1076748 mem = 536879385 peak = 1107369984
[M::mem_process_seqs] Processed 679656 reads in 130.281 CPU sec, 4.181 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287040, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 287, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (312.37, 146.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 90, #reads = 1074843 mem = 536878540 peak = 1107369984
[M::process] read 680940 sequences (100000246 bp) in 2.952 CPU sec, 3.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287626, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 295, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (319.87, 150.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680130 sequences (100000050 bp) in 2.118 CPU sec, 2.187 real sec...
[M::mem_process_seqs] Processed 681634 reads in 197.561 CPU sec, 6.374 real sec
[M::mem_process_seqs] Processed 680818 reads in 129.007 CPU sec, 4.125 real sec
sorting block 91, #reads = 1075924 mem = 536879299 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287452, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 293, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (316.73, 147.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679586 sequences (100000145 bp) in 2.696 CPU sec, 3.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286627, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 299, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (320.61, 147.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679400 sequences (100000123 bp) in 2.019 CPU sec, 2.066 real sec...
[M::mem_process_seqs] Processed 680940 reads in 199.484 CPU sec, 6.425 real sec
[M::mem_process_seqs] Processed 680130 reads in 130.440 CPU sec, 4.177 real sec
sorting block 92, #reads = 1075260 mem = 536878441 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286664, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (325.17, 149.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678638 sequences (100000255 bp) in 2.955 CPU sec, 3.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286963, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (328.53, 152.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678154 sequences (100000063 bp) in 2.107 CPU sec, 2.111 real sec...
[M::mem_process_seqs] Processed 679586 reads in 197.090 CPU sec, 6.306 real sec
sorting block 93, #reads = 1074138 mem = 536877323 peak = 1107369984
[M::mem_process_seqs] Processed 679400 reads in 127.680 CPU sec, 4.073 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286335, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (333.06, 153.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 94, #reads = 1075191 mem = 536880666 peak = 1107369984
[M::process] read 677784 sequences (100000160 bp) in 2.963 CPU sec, 3.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286321, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 313, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (334.14, 152.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 292, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (229, 334)
[M::mem_pestat] mean and std.dev: (275.14, 18.36)
[M::mem_pestat] low and high boundaries for proper pairs: (202, 355)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 678638 reads in 187.693 CPU sec, 5.998 real sec
[M::process] read 677790 sequences (100000240 bp) in 2.222 CPU sec, 2.499 real sec...
[M::mem_process_seqs] Processed 678154 reads in 123.036 CPU sec, 3.907 real sec
sorting block 95, #reads = 1076408 mem = 536881703 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286010, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (336.75, 153.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2169, 2169, 2169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2169, 2169)
[M::mem_pestat] mean and std.dev: (2169.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (2169, 2169)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677458 sequences (100000190 bp) in 2.662 CPU sec, 2.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286187, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (338.91, 156.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677456 sequences (100000234 bp) in 1.983 CPU sec, 1.993 real sec...
[M::mem_process_seqs] Processed 677784 reads in 181.657 CPU sec, 5.791 real sec
[M::mem_process_seqs] Processed 677790 reads in 118.820 CPU sec, 3.763 real sec
sorting block 96, #reads = 1076724 mem = 536880511 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285753, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (341.20, 157.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677280 sequences (100000078 bp) in 3.083 CPU sec, 3.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285846, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (344.52, 160.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677458 reads in 181.393 CPU sec, 5.858 real sec
[M::process] read 676962 sequences (100000173 bp) in 2.000 CPU sec, 2.026 real sec...
[M::mem_process_seqs] Processed 677456 reads in 119.036 CPU sec, 3.838 real sec
sorting block 97, #reads = 1077031 mem = 536878710 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285910, 12, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (344.71, 159.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 348, 1613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4789)
[M::mem_pestat] mean and std.dev: (728.00, 782.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6377)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676738 sequences (100000035 bp) in 2.937 CPU sec, 3.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285451, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (343.40, 157.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676262 sequences (100000059 bp) in 1.900 CPU sec, 1.906 real sec...
[M::mem_process_seqs] Processed 677280 reads in 184.132 CPU sec, 5.893 real sec
sorting block 98, #reads = 1077128 mem = 536879404 peak = 1107369984
[M::mem_process_seqs] Processed 676962 reads in 117.338 CPU sec, 3.728 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285646, 1, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (348.94, 160.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (494, 494, 4144)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11444)
[M::mem_pestat] mean and std.dev: (1945.93, 1805.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15094)
[M::mem_pestat] skip orientation RR
sorting block 99, #reads = 1078092 mem = 536877070 peak = 1107369984
[M::process] read 676234 sequences (100000001 bp) in 3.058 CPU sec, 3.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285188, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (350.77, 161.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676738 reads in 185.237 CPU sec, 5.933 real sec
[M::process] read 675996 sequences (100000127 bp) in 1.934 CPU sec, 2.036 real sec...
[M::mem_process_seqs] Processed 676262 reads in 117.499 CPU sec, 3.739 real sec
sorting block 100, #reads = 1077042 mem = 536877414 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285333, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (351.30, 161.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676152 sequences (100000245 bp) in 2.998 CPU sec, 3.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284897, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (354.08, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676234 reads in 182.466 CPU sec, 5.851 real sec
[M::process] read 676284 sequences (100000056 bp) in 1.965 CPU sec, 1.978 real sec...
[M::mem_process_seqs] Processed 675996 reads in 116.801 CPU sec, 3.716 real sec
sorting block 101, #reads = 1077280 mem = 536878210 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285334, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 218, 1391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3779)
[M::mem_pestat] mean and std.dev: (491.69, 499.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4973)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (352.57, 163.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675970 sequences (100000286 bp) in 2.793 CPU sec, 3.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285599, 12, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (352.60, 164.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 1866, 2489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7087)
[M::mem_pestat] mean and std.dev: (1311.92, 1017.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9386)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676152 reads in 181.080 CPU sec, 5.792 real sec
[M::process] read 676344 sequences (100000238 bp) in 1.907 CPU sec, 1.917 real sec...
sorting block 102, #reads = 1077680 mem = 536880149 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285261, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (354.86, 165.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676184 sequences (100000212 bp) in 2.318 CPU sec, 2.447 real sec...
[M::mem_process_seqs] Processed 675970 reads in 106.390 CPU sec, 3.514 real sec
[M::mem_process_seqs] Processed 676284 reads in 259.256 CPU sec, 8.395 real sec
sorting block 103, #reads = 1078266 mem = 536882848 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285172, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (353.37, 164.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676048 sequences (100000074 bp) in 2.565 CPU sec, 2.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285272, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 345, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (271.60, 109.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (356.10, 167.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 358, 719)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1705)
[M::mem_pestat] mean and std.dev: (364.75, 212.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2198)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676344 reads in 203.950 CPU sec, 6.556 real sec
[M::mem_process_seqs] Processed 676184 reads in 120.274 CPU sec, 3.863 real sec
[M::process] read 676898 sequences (100000214 bp) in 2.587 CPU sec, 2.690 real sec...
sorting block 104, #reads = 1077627 mem = 536878317 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285330, 11, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (528, 528, 825)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419)
[M::mem_pestat] mean and std.dev: (575.50, 239.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1716)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (359.19, 169.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (307, 311, 2365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6481)
[M::mem_pestat] mean and std.dev: (909.64, 920.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8539)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 680422 sequences (100000242 bp) in 2.359 CPU sec, 2.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284740, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (350.57, 165.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680478 sequences (100000180 bp) in 1.960 CPU sec, 1.968 real sec...
[M::mem_process_seqs] Processed 676048 reads in 204.354 CPU sec, 6.590 real sec
sorting block 105, #reads = 1077013 mem = 536876362 peak = 1107369984
[M::mem_process_seqs] Processed 676898 reads in 140.991 CPU sec, 4.542 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287170, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 297, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (322.84, 153.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 106, #reads = 1069107 mem = 536878664 peak = 1107369984
[M::process] read 680128 sequences (100000026 bp) in 3.116 CPU sec, 3.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286716, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 297, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (323.42, 154.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679302 sequences (100000286 bp) in 1.994 CPU sec, 1.995 real sec...
[M::mem_process_seqs] Processed 680422 reads in 187.925 CPU sec, 6.057 real sec
[M::mem_process_seqs] Processed 680478 reads in 116.600 CPU sec, 3.754 real sec
sorting block 107, #reads = 1079265 mem = 536878923 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286991, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 300, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (325.39, 154.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 275, 2103)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5759)
[M::mem_pestat] mean and std.dev: (937.00, 787.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7587)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 679334 sequences (100000113 bp) in 2.709 CPU sec, 2.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286530, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (331.17, 157.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680128 reads in 183.798 CPU sec, 5.900 real sec
[M::process] read 678458 sequences (100000186 bp) in 2.356 CPU sec, 2.390 real sec...
[M::mem_process_seqs] Processed 679302 reads in 114.993 CPU sec, 3.664 real sec
sorting block 108, #reads = 1079838 mem = 536881828 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286397, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (333.53, 158.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677692 sequences (100000164 bp) in 2.791 CPU sec, 3.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286430, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (336.17, 158.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679334 reads in 177.217 CPU sec, 5.738 real sec
[M::mem_process_seqs] Processed 678458 reads in 113.767 CPU sec, 3.648 real sec
[M::process] read 677846 sequences (100000117 bp) in 2.072 CPU sec, 2.149 real sec...
sorting block 109, #reads = 1080545 mem = 536879529 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286206, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (338.79, 157.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677488 sequences (100000160 bp) in 2.582 CPU sec, 2.805 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286038, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (337.16, 157.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677692 reads in 173.308 CPU sec, 5.538 real sec
[M::process] read 677580 sequences (100000064 bp) in 2.080 CPU sec, 2.114 real sec...
[M::mem_process_seqs] Processed 677846 reads in 112.595 CPU sec, 3.563 real sec
sorting block 110, #reads = 1081146 mem = 536879253 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285818, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (340.87, 158.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 111, #reads = 1081673 mem = 536880113 peak = 1107369984
[M::process] read 677450 sequences (100000051 bp) in 2.875 CPU sec, 3.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286377, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (338.88, 156.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677488 reads in 174.736 CPU sec, 5.610 real sec
[M::mem_process_seqs] Processed 677580 reads in 114.195 CPU sec, 3.611 real sec
[M::process] read 677448 sequences (100000058 bp) in 2.338 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286092, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 319, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (339.67, 155.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 112, #reads = 1081247 mem = 536879036 peak = 1107369984
[M::process] read 677388 sequences (100000220 bp) in 2.445 CPU sec, 2.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285920, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 319, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (339.48, 156.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677450 reads in 178.897 CPU sec, 5.754 real sec
[M::mem_process_seqs] Processed 677448 reads in 115.567 CPU sec, 3.684 real sec
[M::process] read 677476 sequences (100000089 bp) in 2.066 CPU sec, 2.164 real sec...
sorting block 113, #reads = 1080528 mem = 536880164 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285735, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 320, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (340.52, 156.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677666 sequences (100000002 bp) in 2.946 CPU sec, 3.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286114, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (340.63, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677388 reads in 180.157 CPU sec, 5.770 real sec
[M::mem_process_seqs] Processed 677476 reads in 118.649 CPU sec, 3.756 real sec
[M::process] read 677902 sequences (100000287 bp) in 2.183 CPU sec, 2.223 real sec...
sorting block 114, #reads = 1079545 mem = 536880454 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285750, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (338.72, 157.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677808 sequences (100000218 bp) in 2.621 CPU sec, 2.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285584, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (335.11, 155.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677666 reads in 182.539 CPU sec, 5.853 real sec
[M::process] read 677832 sequences (100000216 bp) in 2.056 CPU sec, 2.072 real sec...
[M::mem_process_seqs] Processed 677902 reads in 118.558 CPU sec, 3.755 real sec
sorting block 115, #reads = 1079067 mem = 536878366 peak = 1107369984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286080, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (336.62, 156.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 400, 1957)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5393)
[M::mem_pestat] mean and std.dev: (881.33, 773.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7111)
[M::mem_pestat] skip orientation RR
sorting block 116, #reads = 1079380 mem = 536883299 peak = 1115996160
[M::process] read 676886 sequences (100000269 bp) in 2.644 CPU sec, 2.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286124, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 316, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (339.07, 157.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677808 reads in 183.403 CPU sec, 5.899 real sec
[M::process] read 676922 sequences (100000146 bp) in 2.039 CPU sec, 2.058 real sec...
[M::mem_process_seqs] Processed 677832 reads in 119.459 CPU sec, 3.827 real sec
sorting block 117, #reads = 1079312 mem = 536880454 peak = 1115996160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285844, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (348.87, 163.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676708 sequences (100000070 bp) in 2.196 CPU sec, 2.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285436, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (349.77, 164.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676612 sequences (100000291 bp) in 2.096 CPU sec, 2.104 real sec...
[M::mem_process_seqs] Processed 676886 reads in 182.274 CPU sec, 5.861 real sec
[M::mem_process_seqs] Processed 676922 reads in 118.671 CPU sec, 3.786 real sec
sorting block 118, #reads = 1078771 mem = 536878793 peak = 1115996160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285122, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 328, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.49, 163.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676226 sequences (100000271 bp) in 2.848 CPU sec, 3.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284983, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (351.36, 163.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676708 reads in 188.917 CPU sec, 6.073 real sec
[M::process] read 676178 sequences (100000059 bp) in 2.029 CPU sec, 2.039 real sec...
[M::mem_process_seqs] Processed 676612 reads in 124.877 CPU sec, 3.966 real sec
sorting block 119, #reads = 1078066 mem = 536879300 peak = 1115996160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285599, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (353.17, 164.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 201, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (201, 201)
[M::mem_pestat] mean and std.dev: (201.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (201, 201)
[M::mem_pestat] skip orientation RR
[M::process] read 677066 sequences (100000145 bp) in 2.620 CPU sec, 3.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285110, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (354.79, 166.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676226 reads in 179.708 CPU sec, 5.791 real sec
[M::process] read 676474 sequences (100000252 bp) in 2.133 CPU sec, 2.162 real sec...
sorting block 120, #reads = 1076807 mem = 536878045 peak = 1115996160
[M::mem_process_seqs] Processed 676178 reads in 119.730 CPU sec, 3.795 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285770, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 319, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (345.67, 164.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 121, #reads = 1077512 mem = 536878444 peak = 1115996160
[M::process] read 678914 sequences (100000115 bp) in 2.847 CPU sec, 3.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284861, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (283, 337, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225)
[M::mem_pestat] mean and std.dev: (485.73, 264.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1545)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 328, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (352.87, 167.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682166 sequences (100000258 bp) in 2.077 CPU sec, 2.085 real sec...
[M::mem_process_seqs] Processed 677066 reads in 191.216 CPU sec, 6.153 real sec
[M::mem_process_seqs] Processed 676474 reads in 127.132 CPU sec, 4.072 real sec
sorting block 122, #reads = 1076240 mem = 536879174 peak = 1115996160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286270, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (336.54, 160.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681840 sequences (100000028 bp) in 2.342 CPU sec, 2.606 real sec...
[M::process] read 682298 sequences (100000140 bp) in 2.060 CPU sec, 2.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287063, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 286, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (311.75, 146.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678914 reads in 208.973 CPU sec, 6.754 real sec
[M::mem_process_seqs] Processed 682166 reads in 130.803 CPU sec, 4.213 real sec
sorting block 123, #reads = 1069749 mem = 536879951 peak = 1115996160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287356, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 288, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (313.18, 147.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681576 sequences (100000111 bp) in 2.819 CPU sec, 3.175 real sec...
[M::process] read 681286 sequences (100000078 bp) in 2.060 CPU sec, 2.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287786, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 302, 839)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2155)
[M::mem_pestat] mean and std.dev: (471.50, 332.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2813)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 285, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (309.40, 144.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 681840 reads in 201.260 CPU sec, 6.482 real sec
[M::mem_process_seqs] Processed 682298 reads in 132.343 CPU sec, 4.220 real sec
sorting block 124, #reads = 1076425 mem = 536878912 peak = 1115996160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287136, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 289, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (313.82, 146.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680452 sequences (100000268 bp) in 2.602 CPU sec, 3.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286980, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 292, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (314.81, 145.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679820 sequences (100000184 bp) in 2.026 CPU sec, 2.030 real sec...
[M::mem_process_seqs] Processed 681576 reads in 200.872 CPU sec, 6.480 real sec
sorting block 125, #reads = 1074455 mem = 536877227 peak = 1115996160
[M::mem_process_seqs] Processed 681286 reads in 130.969 CPU sec, 4.167 real sec
sorting block 126, #reads = 1074866 mem = 536884967 peak = 1147428864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287148, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 297, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (319.76, 147.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679078 sequences (100000066 bp) in 2.776 CPU sec, 3.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286454, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 303, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (325.11, 150.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678836 sequences (100000284 bp) in 2.009 CPU sec, 2.051 real sec...
[M::mem_process_seqs] Processed 680452 reads in 195.128 CPU sec, 6.287 real sec
[M::mem_process_seqs] Processed 679820 reads in 124.988 CPU sec, 4.001 real sec
sorting block 127, #reads = 1073712 mem = 536878025 peak = 1147428864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286967, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (330.58, 152.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 281, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 628)
[M::mem_pestat] mean and std.dev: (236.00, 92.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation RR
[M::process] read 678618 sequences (100000101 bp) in 2.562 CPU sec, 2.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286782, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (333.51, 154.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679078 reads in 185.908 CPU sec, 6.047 real sec
[M::process] read 678528 sequences (100000008 bp) in 2.158 CPU sec, 2.266 real sec...
[M::mem_process_seqs] Processed 678836 reads in 119.607 CPU sec, 3.893 real sec
sorting block 128, #reads = 1077431 mem = 536878148 peak = 1147428864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286221, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (334.16, 154.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678408 sequences (100000104 bp) in 2.782 CPU sec, 3.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286538, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (336.02, 155.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678618 reads in 184.292 CPU sec, 5.958 real sec
[M::process] read 678038 sequences (100000076 bp) in 1.950 CPU sec, 1.968 real sec...
[M::mem_process_seqs] Processed 678528 reads in 117.107 CPU sec, 3.743 real sec
sorting block 129, #reads = 1078241 mem = 536878687 peak = 1147428864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286180, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 316, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (337.73, 157.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 130, #reads = 1078937 mem = 536878294 peak = 1147428864
[M::process] read 677774 sequences (100000078 bp) in 2.799 CPU sec, 2.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285276, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (342.15, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678408 reads in 187.295 CPU sec, 6.033 real sec
[M::process] read 677532 sequences (100000127 bp) in 1.906 CPU sec, 1.914 real sec...
[M::mem_process_seqs] Processed 678038 reads in 118.462 CPU sec, 3.815 real sec
sorting block 131, #reads = 1079066 mem = 536881007 peak = 1147428864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285918, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (341.84, 158.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677112 sequences (100000170 bp) in 3.161 CPU sec, 3.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286032, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (344.15, 159.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677210 sequences (100000063 bp) in 1.920 CPU sec, 1.943 real sec...
[M::mem_process_seqs] Processed 677774 reads in 188.616 CPU sec, 6.066 real sec
[M::mem_process_seqs] Processed 677532 reads in 117.615 CPU sec, 3.776 real sec
sorting block 132, #reads = 1077577 mem = 536881269 peak = 1147428864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285814, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (344.43, 158.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677006 sequences (100000295 bp) in 2.711 CPU sec, 3.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285492, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 323, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (343.01, 156.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677112 reads in 186.026 CPU sec, 6.012 real sec
[M::process] read 676612 sequences (100000283 bp) in 1.916 CPU sec, 1.929 real sec...
[M::mem_process_seqs] Processed 677210 reads in 117.288 CPU sec, 3.742 real sec
sorting block 133, #reads = 1078317 mem = 536877667 peak = 1147428864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285084, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (905, 1104, 2888)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6854)
[M::mem_pestat] mean and std.dev: (1528.50, 989.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8837)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.49, 160.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676182 sequences (100000230 bp) in 2.743 CPU sec, 3.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285353, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.43, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677006 reads in 184.685 CPU sec, 5.937 real sec
[M::process] read 676044 sequences (100000135 bp) in 1.998 CPU sec, 2.038 real sec...
sorting block 134, #reads = 1076929 mem = 536880616 peak = 1147428864
[M::mem_process_seqs] Processed 676612 reads in 117.932 CPU sec, 3.755 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285189, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.60, 162.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 135, #reads = 1077264 mem = 536879248 peak = 1147428864
[M::process] read 676306 sequences (100000194 bp) in 2.609 CPU sec, 3.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285557, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (353.12, 161.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676182 reads in 180.054 CPU sec, 5.854 real sec
[M::process] read 676080 sequences (100000157 bp) in 1.968 CPU sec, 1.990 real sec...
[M::mem_process_seqs] Processed 676044 reads in 113.588 CPU sec, 3.676 real sec
sorting block 136, #reads = 1079271 mem = 536879171 peak = 1147428864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285368, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 403, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1884)
[M::mem_pestat] mean and std.dev: (398.42, 233.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2439)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (349.35, 159.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675576 sequences (100000184 bp) in 3.026 CPU sec, 3.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284887, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 368, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (299.33, 108.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (349.05, 159.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676306 reads in 178.777 CPU sec, 5.747 real sec
[M::process] read 619590 sequences (91616768 bp) in 1.922 CPU sec, 1.949 real sec...
[M::mem_process_seqs] Processed 676080 reads in 117.043 CPU sec, 3.730 real sec
sorting block 137, #reads = 1078473 mem = 536877447 peak = 1147428864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284949, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.02, 159.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 261088, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (347.20, 159.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675576 reads in 170.225 CPU sec, 5.557 real sec
[M::mem_process_seqs] Processed 619590 reads in 110.517 CPU sec, 3.583 real sec
sorting block 138, #reads = 1078400 mem = 536882171 peak = 1147428864
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 150232352
overall: 69862920192 mem 22982.413 user 499.094 sys 775.056 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_d0M-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tLB:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 775.090 sec; CPU: 23481.510 sec
sorting block 139, #reads = 921613 mem = 459833681 peak = 1147428864
uniq header count 1
merging 140 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8333481101
output reads: 150966597
bam_mem_sort: 140 calls 101.799 user 2.002 sys 107.941 real
bam_write: 140 calls 88.665 user 6.505 sys 190.713 real
execute: 1 calls 48.922 user 2.269 sys 837.658 real
merge_files: 1 calls 48.888 user 2.257 sys 61.792 real
parse_chunk: 2238 calls 104.281 user 28.349 sys 175.494 real
read_chunk: 6030 calls 58.270 user 31.055 sys 730.184 real
sort_block: 1 calls 38.332 user 29.933 sys 699.716 real
write_chunk: 3790 calls 48.253 user 1.953 sys 60.204 real
overall: 3147292672 mem 2317.842 user 94.616 sys 837.678 real
ls: cannot access '*.cram': No such file or directory