.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L003;0004)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/51/bcf89a18224246f10fd98cf838a42f/.command.err
Size: 133.2 KB
Task: DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L003;0004)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1694.289 CPU sec, 1026.069 real sec
[M::process] read 701768 sequences (100000125 bp) in 0.964 CPU sec, 0.971 real sec...
[M::process] read 701572 sequences (100000270 bp) in 1.250 CPU sec, 1.251 real sec...
[M::process] read 701710 sequences (100000241 bp) in 1.258 CPU sec, 1.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291599, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (239.98, 113.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701632 sequences (100000257 bp) in 1.198 CPU sec, 1.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291632, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.86, 114.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291291, 5, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (96, 96, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (200.00, 200.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (241.37, 114.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701768 reads in 209.872 CPU sec, 6.669 real sec
[M::mem_process_seqs] Processed 701572 reads in 137.145 CPU sec, 4.402 real sec
[M::mem_process_seqs] Processed 701710 reads in 148.558 CPU sec, 4.788 real sec
sorting block 0, #reads = 1095993 mem = 536885423 peak = 592994304
[M::process] read 701954 sequences (100000096 bp) in 1.898 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292195, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (311, 1653, 1653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4337)
[M::mem_pestat] mean and std.dev: (1022.67, 651.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5679)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.66, 116.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699720 sequences (100000250 bp) in 1.300 CPU sec, 1.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291916, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.54, 114.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701632 reads in 128.923 CPU sec, 4.099 real sec
sorting block 1, #reads = 1094985 mem = 536878875 peak = 767356928
[M::mem_process_seqs] Processed 701954 reads in 81.847 CPU sec, 2.585 real sec
[M::process] read 699000 sequences (100000140 bp) in 1.524 CPU sec, 1.559 real sec...
sorting block 2, #reads = 1096193 mem = 536880357 peak = 870191104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291703, 11, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 382, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (294.00, 151.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.45, 118.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 159, 2551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7335)
[M::mem_pestat] mean and std.dev: (1242.55, 1573.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9727)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 697996 sequences (100000051 bp) in 1.848 CPU sec, 1.930 real sec...
[M::process] read 697774 sequences (100000145 bp) in 1.280 CPU sec, 1.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291146, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.45, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699720 reads in 126.657 CPU sec, 4.023 real sec
[M::mem_process_seqs] Processed 699000 reads in 79.360 CPU sec, 2.521 real sec
sorting block 3, #reads = 1095313 mem = 536883921 peak = 870191104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290934, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.67, 121.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698300 sequences (100000125 bp) in 1.882 CPU sec, 1.991 real sec...
[M::process] read 696304 sequences (100000027 bp) in 1.240 CPU sec, 1.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290853, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.73, 121.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (294, 555, 769)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1719)
[M::mem_pestat] mean and std.dev: (549.09, 332.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2194)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 697996 reads in 127.536 CPU sec, 4.056 real sec
[M::mem_process_seqs] Processed 697774 reads in 80.279 CPU sec, 2.547 real sec
sorting block 4, #reads = 1094013 mem = 536878513 peak = 870191104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290782, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (249.06, 118.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 327, 1953)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5463)
[M::mem_pestat] mean and std.dev: (897.64, 1380.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7218)
[M::mem_pestat] skip orientation RR
[M::process] read 696358 sequences (100000008 bp) in 1.868 CPU sec, 1.990 real sec...
[M::process] read 695420 sequences (100000105 bp) in 1.270 CPU sec, 1.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 290163, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 236, 1411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3815)
[M::mem_pestat] mean and std.dev: (663.14, 717.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5017)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.77, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698300 reads in 127.511 CPU sec, 4.053 real sec
sorting block 5, #reads = 1094022 mem = 536880895 peak = 870191104
[M::mem_process_seqs] Processed 696304 reads in 79.635 CPU sec, 2.528 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290621, 9, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (421.14, 264.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1481)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (255.85, 121.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 6, #reads = 1093794 mem = 536877942 peak = 870191104
[M::process] read 695992 sequences (100000261 bp) in 2.091 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289566, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 234, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (256.27, 120.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696104 sequences (100000210 bp) in 1.330 CPU sec, 1.326 real sec...
[M::mem_process_seqs] Processed 696358 reads in 128.134 CPU sec, 4.074 real sec
[M::process] read 695568 sequences (100000274 bp) in 1.469 CPU sec, 1.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289872, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (254.12, 119.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695992 reads in 75.160 CPU sec, 2.577 real sec
sorting block 7, #reads = 1093661 mem = 536878116 peak = 870191104
[M::mem_process_seqs] Processed 695420 reads in 176.226 CPU sec, 5.790 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290343, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.89, 119.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695152 sequences (100000184 bp) in 2.079 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289758, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.48, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696104 reads in 139.813 CPU sec, 4.447 real sec
sorting block 8, #reads = 1092116 mem = 536877457 peak = 870191104
[M::mem_process_seqs] Processed 695568 reads in 82.315 CPU sec, 2.599 real sec
[M::process] read 694618 sequences (100000133 bp) in 1.569 CPU sec, 1.614 real sec...
sorting block 9, #reads = 1092907 mem = 536880290 peak = 870191104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289621, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 1031, 1536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4112)
[M::mem_pestat] mean and std.dev: (752.78, 559.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5400)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 234, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (256.40, 120.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701606 sequences (100000035 bp) in 2.068 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289402, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 235, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (257.70, 121.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (351, 405, 710)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428)
[M::mem_pestat] mean and std.dev: (461.91, 236.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1787)
[M::mem_pestat] skip orientation RR
[M::process] read 701742 sequences (100000207 bp) in 1.350 CPU sec, 1.353 real sec...
[M::mem_process_seqs] Processed 695152 reads in 128.877 CPU sec, 4.104 real sec
[M::mem_process_seqs] Processed 694618 reads in 81.138 CPU sec, 2.575 real sec
sorting block 10, #reads = 1092035 mem = 536882060 peak = 870191104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291755, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.49, 116.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701632 sequences (100000243 bp) in 2.335 CPU sec, 2.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291772, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.21, 115.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701606 reads in 130.427 CPU sec, 4.150 real sec
[M::process] read 700956 sequences (100000021 bp) in 1.472 CPU sec, 1.475 real sec...
[M::mem_process_seqs] Processed 701742 reads in 81.868 CPU sec, 2.590 real sec
sorting block 11, #reads = 1092572 mem = 536878733 peak = 870191104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291799, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.82, 116.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700556 sequences (100000135 bp) in 2.269 CPU sec, 2.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291897, 9, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 136, 717)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913)
[M::mem_pestat] mean and std.dev: (382.00, 401.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.60, 117.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 210, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (264.59, 141.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701632 reads in 128.384 CPU sec, 4.084 real sec
[M::process] read 699982 sequences (100000286 bp) in 1.531 CPU sec, 1.550 real sec...
sorting block 12, #reads = 1097013 mem = 536880723 peak = 870191104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291672, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 221, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (231.56, 97.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.37, 118.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698774 sequences (100000192 bp) in 1.601 CPU sec, 1.609 real sec...
[M::mem_process_seqs] Processed 700556 reads in 75.656 CPU sec, 2.589 real sec
sorting block 13, #reads = 1095558 mem = 536878322 peak = 877355008
[M::mem_process_seqs] Processed 700956 reads in 177.018 CPU sec, 5.796 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291412, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.41, 120.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697924 sequences (100000163 bp) in 2.318 CPU sec, 2.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291371, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 469, 707)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629)
[M::mem_pestat] mean and std.dev: (398.73, 187.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2090)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.42, 122.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699982 reads in 140.083 CPU sec, 4.459 real sec
[M::mem_process_seqs] Processed 698774 reads in 81.708 CPU sec, 2.585 real sec
sorting block 14, #reads = 1095439 mem = 536878901 peak = 877355008
[M::process] read 698204 sequences (100000060 bp) in 1.929 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290643, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.26, 123.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1095278 mem = 536879957 peak = 886865920
[M::process] read 697228 sequences (100000032 bp) in 2.105 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290837, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.07, 122.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697924 reads in 129.605 CPU sec, 4.133 real sec
[M::mem_process_seqs] Processed 698204 reads in 82.507 CPU sec, 2.602 real sec
[M::process] read 696198 sequences (100000027 bp) in 1.804 CPU sec, 1.866 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 290583, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 328, 618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456)
[M::mem_pestat] mean and std.dev: (359.47, 250.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1875)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 16, #reads = 1093322 mem = 536882769 peak = 886865920
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.52, 122.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696266 sequences (100000141 bp) in 2.154 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290152, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 231, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (317.30, 126.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.45, 122.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697228 reads in 129.252 CPU sec, 4.118 real sec
[M::mem_process_seqs] Processed 696198 reads in 82.066 CPU sec, 2.590 real sec
[M::process] read 695858 sequences (100000295 bp) in 1.637 CPU sec, 1.685 real sec...
sorting block 17, #reads = 1093922 mem = 536877539 peak = 886865920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290347, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 186, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 376)
[M::mem_pestat] mean and std.dev: (181.00, 35.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.34, 121.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695658 sequences (100000040 bp) in 2.174 CPU sec, 2.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289958, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 238, 708)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718)
[M::mem_pestat] mean and std.dev: (449.30, 337.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2223)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (257.64, 122.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696266 reads in 128.925 CPU sec, 4.105 real sec
[M::mem_process_seqs] Processed 695858 reads in 82.146 CPU sec, 2.595 real sec
[M::process] read 695926 sequences (100000150 bp) in 1.590 CPU sec, 1.632 real sec...
sorting block 18, #reads = 1092893 mem = 536881165 peak = 890806272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289923, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.42, 120.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (195.80, 58.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] skip orientation RR
[M::process] read 696018 sequences (100000241 bp) in 2.278 CPU sec, 2.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290555, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.55, 120.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 236, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (250.75, 72.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695658 reads in 130.043 CPU sec, 4.142 real sec
sorting block 19, #reads = 1091577 mem = 536878635 peak = 890806272
[M::mem_process_seqs] Processed 695926 reads in 82.975 CPU sec, 2.616 real sec
[M::process] read 695644 sequences (100000085 bp) in 1.583 CPU sec, 1.616 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290573, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.25, 119.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1091974 mem = 536881130 peak = 966787072
[M::process] read 695756 sequences (100000225 bp) in 2.258 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289923, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (422, 472, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (322, 572)
[M::mem_pestat] mean and std.dev: (450.14, 25.26)
[M::mem_pestat] low and high boundaries for proper pairs: (272, 622)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (255.18, 120.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 251, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 572)
[M::mem_pestat] mean and std.dev: (247.22, 78.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 683)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696018 reads in 129.736 CPU sec, 4.128 real sec
[M::process] read 700656 sequences (100000029 bp) in 1.540 CPU sec, 1.556 real sec...
[M::mem_process_seqs] Processed 695644 reads in 82.431 CPU sec, 2.599 real sec
sorting block 21, #reads = 1092546 mem = 536879121 peak = 966787072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290327, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (255.90, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702366 sequences (100000050 bp) in 2.372 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291591, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.33, 116.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695756 reads in 131.163 CPU sec, 4.173 real sec
[M::mem_process_seqs] Processed 700656 reads in 83.577 CPU sec, 2.635 real sec
[M::process] read 701492 sequences (100000010 bp) in 1.613 CPU sec, 1.642 real sec...
sorting block 22, #reads = 1091844 mem = 536877148 peak = 966787072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292282, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.69, 115.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (250.60, 153.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RR
[M::process] read 700958 sequences (100000015 bp) in 2.362 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291969, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.87, 116.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702366 reads in 131.769 CPU sec, 4.192 real sec
[M::mem_process_seqs] Processed 701492 reads in 83.963 CPU sec, 2.647 real sec
[M::process] read 700150 sequences (100000020 bp) in 1.602 CPU sec, 1.632 real sec...
sorting block 23, #reads = 1094773 mem = 536880461 peak = 966787072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291878, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.48, 117.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1095621 mem = 536885310 peak = 966787072
[M::process] read 699740 sequences (100000034 bp) in 2.318 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291902, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 284, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (226.64, 84.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.67, 118.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700958 reads in 131.917 CPU sec, 4.206 real sec
[M::mem_process_seqs] Processed 700150 reads in 83.192 CPU sec, 2.628 real sec
[M::process] read 699230 sequences (100000066 bp) in 1.676 CPU sec, 1.692 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290900, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.49, 119.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1093963 mem = 536880205 peak = 966787072
[M::process] read 698350 sequences (100000126 bp) in 2.371 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291290, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.78, 121.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699740 reads in 130.024 CPU sec, 4.142 real sec
[M::mem_process_seqs] Processed 699230 reads in 82.558 CPU sec, 2.604 real sec
[M::process] read 698170 sequences (100000253 bp) in 1.760 CPU sec, 1.818 real sec...
sorting block 26, #reads = 1094752 mem = 536885019 peak = 966787072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290980, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.94, 122.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697806 sequences (100000129 bp) in 2.258 CPU sec, 2.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291025, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 324, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (239.70, 95.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.17, 122.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698350 reads in 129.507 CPU sec, 4.127 real sec
[M::mem_process_seqs] Processed 698170 reads in 82.299 CPU sec, 2.595 real sec
[M::process] read 697670 sequences (100000222 bp) in 1.751 CPU sec, 1.791 real sec...
sorting block 27, #reads = 1094444 mem = 536881660 peak = 966787072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290727, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.12, 122.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697634 sequences (100000189 bp) in 2.327 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290535, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.46, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697806 reads in 129.610 CPU sec, 4.132 real sec
[M::mem_process_seqs] Processed 697670 reads in 82.709 CPU sec, 2.614 real sec
sorting block 28, #reads = 1093517 mem = 536877762 peak = 966787072
[M::process] read 697212 sequences (100000023 bp) in 1.789 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290613, 3, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.56, 120.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 229, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (256.53, 129.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RR
sorting block 29, #reads = 1092560 mem = 536878709 peak = 1017212928
[M::process] read 697274 sequences (100000077 bp) in 2.347 CPU sec, 2.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290514, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 155, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (190.67, 84.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 634)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.55, 118.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697634 reads in 129.779 CPU sec, 4.144 real sec
[M::mem_process_seqs] Processed 697212 reads in 82.558 CPU sec, 2.610 real sec
[M::process] read 696710 sequences (100000223 bp) in 1.868 CPU sec, 1.935 real sec...
sorting block 30, #reads = 1093995 mem = 536878099 peak = 1017212928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290615, 11, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 262, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (253.00, 68.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.32, 119.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 7, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (61.11, 108.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 696450 sequences (100000060 bp) in 2.232 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289901, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.65, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697274 reads in 131.954 CPU sec, 4.206 real sec
[M::mem_process_seqs] Processed 696710 reads in 84.266 CPU sec, 2.660 real sec
[M::process] read 695886 sequences (100000181 bp) in 1.739 CPU sec, 1.804 real sec...
sorting block 31, #reads = 1092410 mem = 536879912 peak = 1017212928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290475, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.58, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695684 sequences (100000087 bp) in 2.376 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289909, 8, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 183, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (72, 282)
[M::mem_pestat] mean and std.dev: (158.50, 35.20)
[M::mem_pestat] low and high boundaries for proper pairs: (18, 324)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.85, 120.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696450 reads in 130.093 CPU sec, 4.146 real sec
[M::mem_process_seqs] Processed 695886 reads in 82.489 CPU sec, 2.624 real sec
sorting block 32, #reads = 1091890 mem = 536876927 peak = 1017212928
[M::process] read 700742 sequences (100000084 bp) in 1.807 CPU sec, 1.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289771, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (254.59, 119.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1092975 mem = 536878535 peak = 1017212928
[M::process] read 702084 sequences (100000041 bp) in 2.328 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291930, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.52, 117.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695684 reads in 132.149 CPU sec, 4.215 real sec
[M::mem_process_seqs] Processed 700742 reads in 84.617 CPU sec, 2.677 real sec
[M::process] read 701384 sequences (100000231 bp) in 1.860 CPU sec, 1.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292152, 8, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (249, 255, 642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428)
[M::mem_pestat] mean and std.dev: (330.55, 192.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1821)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.49, 115.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 34, #reads = 1091976 mem = 536877794 peak = 1017212928
[M::process] read 700686 sequences (100000162 bp) in 2.227 CPU sec, 2.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291793, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.71, 117.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702084 reads in 130.181 CPU sec, 4.152 real sec
[M::mem_process_seqs] Processed 701384 reads in 82.900 CPU sec, 2.619 real sec
[M::process] read 699678 sequences (100000251 bp) in 1.849 CPU sec, 1.920 real sec...
sorting block 35, #reads = 1096574 mem = 536878671 peak = 1017212928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291957, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.61, 118.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699234 sequences (100000023 bp) in 2.233 CPU sec, 2.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291376, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.96, 119.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700686 reads in 129.325 CPU sec, 4.126 real sec
[M::mem_process_seqs] Processed 699678 reads in 81.819 CPU sec, 2.589 real sec
[M::process] read 699020 sequences (100000072 bp) in 1.814 CPU sec, 1.904 real sec...
sorting block 36, #reads = 1095995 mem = 536877614 peak = 1017212928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291196, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (249.94, 120.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698416 sequences (100000228 bp) in 2.334 CPU sec, 2.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291380, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.22, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (566, 3450, 3450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9218)
[M::mem_pestat] mean and std.dev: (2544.20, 2154.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12102)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 699234 reads in 129.225 CPU sec, 4.130 real sec
[M::mem_process_seqs] Processed 699020 reads in 82.324 CPU sec, 2.606 real sec
sorting block 37, #reads = 1095805 mem = 536877292 peak = 1017212928
[M::process] read 698320 sequences (100000044 bp) in 1.905 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290767, 7, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 236, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1685)
[M::mem_pestat] mean and std.dev: (410.71, 389.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2182)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.90, 121.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 421, 748)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1930)
[M::mem_pestat] mean and std.dev: (407.42, 280.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2521)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 38, #reads = 1095113 mem = 536884670 peak = 1043423232
[M::process] read 698016 sequences (100000053 bp) in 2.112 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291175, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.43, 122.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698416 reads in 129.563 CPU sec, 4.130 real sec
[M::mem_process_seqs] Processed 698320 reads in 82.272 CPU sec, 2.600 real sec
[M::process] read 698252 sequences (100000208 bp) in 1.764 CPU sec, 1.819 real sec...
sorting block 39, #reads = 1093935 mem = 536881472 peak = 1043423232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291140, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.18, 121.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698470 sequences (100000066 bp) in 2.307 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291128, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.43, 120.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 266, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (295.11, 104.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698016 reads in 129.109 CPU sec, 4.118 real sec
[M::mem_process_seqs] Processed 698252 reads in 82.234 CPU sec, 2.600 real sec
[M::process] read 698462 sequences (100000018 bp) in 1.883 CPU sec, 1.989 real sec...
sorting block 40, #reads = 1094240 mem = 536879838 peak = 1043423232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291345, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.69, 119.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698302 sequences (100000223 bp) in 2.238 CPU sec, 2.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290752, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 221, 757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2035)
[M::mem_pestat] mean and std.dev: (401.80, 307.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2674)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.72, 117.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698470 reads in 129.803 CPU sec, 4.142 real sec
[M::mem_process_seqs] Processed 698462 reads in 82.507 CPU sec, 2.607 real sec
sorting block 41, #reads = 1095021 mem = 536879452 peak = 1043423232
[M::process] read 697756 sequences (100000264 bp) in 1.922 CPU sec, 2.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290877, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (185.33, 67.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (247.87, 118.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 1126)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3064)
[M::mem_pestat] mean and std.dev: (493.82, 494.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4033)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697224 sequences (100000052 bp) in 2.216 CPU sec, 2.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290453, 10, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.93, 118.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 388, 829)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2079)
[M::mem_pestat] mean and std.dev: (456.20, 338.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2704)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 1660, 1746)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4896)
[M::mem_pestat] mean and std.dev: (1001.80, 838.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6471)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698302 reads in 130.931 CPU sec, 4.179 real sec
sorting block 42, #reads = 1094485 mem = 536884497 peak = 1043423232
[M::mem_process_seqs] Processed 697756 reads in 83.336 CPU sec, 2.636 real sec
[M::process] read 696948 sequences (100000121 bp) in 1.900 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290640, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 292, 1653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4549)
[M::mem_pestat] mean and std.dev: (868.36, 1213.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5997)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.44, 119.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (360, 531, 1500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3780)
[M::mem_pestat] mean and std.dev: (777.91, 643.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4920)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 43, #reads = 1093091 mem = 536879550 peak = 1043423232
[M::process] read 696482 sequences (100000192 bp) in 2.254 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290398, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.00, 119.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697224 reads in 130.804 CPU sec, 4.175 real sec
[M::process] read 701174 sequences (100000093 bp) in 1.983 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290408, 4, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 309, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (256.00, 102.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.59, 119.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696482 reads in 72.320 CPU sec, 2.286 real sec
sorting block 44, #reads = 1092778 mem = 536878972 peak = 1043423232
[M::process] read 706526 sequences (100000053 bp) in 1.869 CPU sec, 1.933 real sec...
[M::mem_process_seqs] Processed 696948 reads in 187.745 CPU sec, 5.947 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291874, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.12, 115.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1091964 mem = 536879849 peak = 1043464192
[M::process] read 705336 sequences (100000093 bp) in 2.343 CPU sec, 2.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293289, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.24, 108.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701174 reads in 150.697 CPU sec, 4.772 real sec
[M::mem_process_seqs] Processed 706526 reads in 88.228 CPU sec, 2.789 real sec
[M::process] read 704222 sequences (100000055 bp) in 1.908 CPU sec, 2.024 real sec...
sorting block 46, #reads = 1093372 mem = 536878395 peak = 1043464192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293435, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (232.29, 111.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704606 sequences (100000105 bp) in 2.084 CPU sec, 2.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292599, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (235.04, 113.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705336 reads in 136.213 CPU sec, 4.335 real sec
[M::process] read 703150 sequences (100000215 bp) in 1.510 CPU sec, 1.537 real sec...
[M::mem_process_seqs] Processed 704222 reads in 85.721 CPU sec, 2.706 real sec
sorting block 47, #reads = 1096606 mem = 536879264 peak = 1043464192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292941, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (232.10, 110.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 749)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702740 sequences (100000022 bp) in 2.322 CPU sec, 2.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292630, 9, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 346, 1425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3929)
[M::mem_pestat] mean and std.dev: (580.92, 581.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5181)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (237.44, 114.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 704606 reads in 133.439 CPU sec, 4.241 real sec
[M::process] read 701326 sequences (100000174 bp) in 1.430 CPU sec, 1.434 real sec...
sorting block 48, #reads = 1096284 mem = 536878367 peak = 1043464192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292415, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (240.32, 116.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702414 sequences (100000218 bp) in 1.666 CPU sec, 1.668 real sec...
[M::mem_process_seqs] Processed 702740 reads in 78.319 CPU sec, 2.710 real sec
sorting block 49, #reads = 1096293 mem = 536881379 peak = 1043464192
[M::mem_process_seqs] Processed 703150 reads in 182.694 CPU sec, 6.012 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292193, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.66, 118.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701092 sequences (100000246 bp) in 2.263 CPU sec, 2.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292301, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (239.10, 115.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701326 reads in 146.702 CPU sec, 4.661 real sec
[M::mem_process_seqs] Processed 702414 reads in 85.201 CPU sec, 2.692 real sec
sorting block 50, #reads = 1094598 mem = 536883417 peak = 1043464192
[M::process] read 701086 sequences (100000196 bp) in 1.785 CPU sec, 1.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291936, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (243.13, 117.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1094715 mem = 536885750 peak = 1066741760
[M::process] read 699404 sequences (100000148 bp) in 1.989 CPU sec, 2.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292160, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.29, 119.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701092 reads in 135.807 CPU sec, 4.324 real sec
[M::process] read 697940 sequences (100000103 bp) in 1.567 CPU sec, 1.580 real sec...
[M::mem_process_seqs] Processed 701086 reads in 85.459 CPU sec, 2.698 real sec
sorting block 52, #reads = 1093128 mem = 536879496 peak = 1066741760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291080, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (249.37, 121.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 388, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (306.50, 112.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RR
[M::process] read 697048 sequences (100000188 bp) in 2.325 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290890, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 171, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (259.00, 172.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.92, 122.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696640 sequences (100000296 bp) in 1.440 CPU sec, 1.447 real sec...
[M::mem_process_seqs] Processed 699404 reads in 137.181 CPU sec, 4.360 real sec
[M::mem_process_seqs] Processed 697940 reads in 86.935 CPU sec, 2.747 real sec
sorting block 53, #reads = 1092270 mem = 536880718 peak = 1066741760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290865, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.45, 122.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696618 sequences (100000243 bp) in 2.368 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290300, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (254.72, 122.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696664 sequences (100000216 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_process_seqs] Processed 697048 reads in 137.013 CPU sec, 4.348 real sec
[M::mem_process_seqs] Processed 696640 reads in 86.291 CPU sec, 2.727 real sec
sorting block 54, #reads = 1090475 mem = 536878627 peak = 1066741760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290280, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.67, 122.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708198 sequences (100000113 bp) in 2.475 CPU sec, 2.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290712, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (256.03, 124.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565)
[M::mem_pestat] mean and std.dev: (270.33, 65.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 679)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696618 reads in 136.292 CPU sec, 4.324 real sec
[M::process] read 707878 sequences (100000263 bp) in 1.639 CPU sec, 1.647 real sec...
sorting block 55, #reads = 1090173 mem = 536879630 peak = 1066741760
[M::mem_process_seqs] Processed 696664 reads in 87.606 CPU sec, 2.759 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 294259, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 202, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (224.83, 105.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1089650 mem = 536877315 peak = 1085595648
[M::process] read 705860 sequences (100000123 bp) in 2.496 CPU sec, 2.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 294166, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 202, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (225.55, 106.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704748 sequences (100000165 bp) in 1.500 CPU sec, 1.505 real sec...
[M::mem_process_seqs] Processed 708198 reads in 147.395 CPU sec, 4.679 real sec
[M::mem_process_seqs] Processed 707878 reads in 94.683 CPU sec, 2.995 real sec
sorting block 57, #reads = 1092344 mem = 536877329 peak = 1085595648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293567, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (231.19, 109.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703354 sequences (100000114 bp) in 2.561 CPU sec, 2.701 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293747, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 470, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (345.40, 149.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1484)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.73, 111.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702750 sequences (100000145 bp) in 1.500 CPU sec, 1.506 real sec...
[M::mem_process_seqs] Processed 705860 reads in 145.423 CPU sec, 4.613 real sec
[M::mem_process_seqs] Processed 704748 reads in 92.499 CPU sec, 2.925 real sec
sorting block 58, #reads = 1091096 mem = 536878989 peak = 1085595648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292796, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.77, 115.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702336 sequences (100000219 bp) in 2.264 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292765, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.67, 116.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703354 reads in 139.657 CPU sec, 4.442 real sec
[M::process] read 701536 sequences (100000061 bp) in 1.500 CPU sec, 1.499 real sec...
sorting block 59, #reads = 1092735 mem = 536878637 peak = 1085595648
[M::mem_process_seqs] Processed 702750 reads in 87.946 CPU sec, 2.778 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292642, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.34, 117.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1092621 mem = 536883184 peak = 1085595648
[M::process] read 700670 sequences (100000115 bp) in 2.579 CPU sec, 2.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292388, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.16, 117.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700874 sequences (100000008 bp) in 1.490 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 702336 reads in 145.971 CPU sec, 4.632 real sec
[M::mem_process_seqs] Processed 701536 reads in 90.941 CPU sec, 2.880 real sec
sorting block 61, #reads = 1089964 mem = 536879582 peak = 1085595648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291707, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.48, 118.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 373, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (330.10, 81.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RR
[M::process] read 699946 sequences (100000261 bp) in 2.268 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292487, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.53, 119.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700670 reads in 138.120 CPU sec, 4.396 real sec
[M::process] read 698464 sequences (100000178 bp) in 1.489 CPU sec, 1.486 real sec...
[M::mem_process_seqs] Processed 700874 reads in 88.169 CPU sec, 2.791 real sec
sorting block 62, #reads = 1092798 mem = 536878534 peak = 1085595648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291889, 9, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 265, 989)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2521)
[M::mem_pestat] mean and std.dev: (425.27, 348.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3287)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.13, 120.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697452 sequences (100000240 bp) in 2.482 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290912, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.46, 123.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (547, 547, 2109)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5233)
[M::mem_pestat] mean and std.dev: (806.50, 696.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6795)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699946 reads in 135.799 CPU sec, 4.311 real sec
[M::process] read 698006 sequences (100000189 bp) in 1.522 CPU sec, 1.535 real sec...
[M::mem_process_seqs] Processed 698464 reads in 85.400 CPU sec, 2.695 real sec
sorting block 63, #reads = 1091612 mem = 536879344 peak = 1085595648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290754, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.91, 123.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698386 sequences (100000132 bp) in 2.502 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291054, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.83, 122.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697452 reads in 135.215 CPU sec, 4.296 real sec
sorting block 64, #reads = 1091878 mem = 536878737 peak = 1085595648
[M::process] read 699282 sequences (100000006 bp) in 1.644 CPU sec, 1.655 real sec...
[M::mem_process_seqs] Processed 698006 reads in 86.209 CPU sec, 2.713 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290905, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.42, 120.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (287, 387, 2241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6149)
[M::mem_pestat] mean and std.dev: (1170.36, 979.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8103)
[M::mem_pestat] skip orientation RR
sorting block 65, #reads = 1091294 mem = 536879256 peak = 1098317824
[M::process] read 706864 sequences (100000268 bp) in 2.429 CPU sec, 2.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290279, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.58, 120.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705602 sequences (100000161 bp) in 1.460 CPU sec, 1.461 real sec...
[M::mem_process_seqs] Processed 698386 reads in 145.425 CPU sec, 4.621 real sec
[M::mem_process_seqs] Processed 699282 reads in 97.578 CPU sec, 3.088 real sec
sorting block 66, #reads = 1090909 mem = 536878230 peak = 1098317824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293542, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (227.70, 107.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703786 sequences (100000091 bp) in 2.327 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293399, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 208, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (231.61, 110.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702854 sequences (100000165 bp) in 1.430 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 706864 reads in 137.847 CPU sec, 4.377 real sec
[M::mem_process_seqs] Processed 705602 reads in 86.388 CPU sec, 2.735 real sec
sorting block 67, #reads = 1087259 mem = 536883430 peak = 1098317824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292696, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (236.45, 113.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702552 sequences (100000170 bp) in 2.452 CPU sec, 2.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292979, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (238.32, 114.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703786 reads in 131.793 CPU sec, 4.189 real sec
[M::process] read 701524 sequences (100000244 bp) in 1.530 CPU sec, 1.539 real sec...
sorting block 68, #reads = 1094446 mem = 536879216 peak = 1098317824
[M::mem_process_seqs] Processed 702854 reads in 83.556 CPU sec, 2.642 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292930, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.20, 116.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1095701 mem = 536878441 peak = 1098317824
[M::process] read 701332 sequences (100000174 bp) in 2.137 CPU sec, 2.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292205, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (755, 1072, 1919)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4247)
[M::mem_pestat] mean and std.dev: (1011.10, 666.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5411)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.22, 117.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702552 reads in 132.089 CPU sec, 4.208 real sec
[M::process] read 701532 sequences (100000112 bp) in 1.620 CPU sec, 1.639 real sec...
[M::mem_process_seqs] Processed 701524 reads in 83.803 CPU sec, 2.642 real sec
sorting block 70, #reads = 1095719 mem = 536880148 peak = 1098317824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292126, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.21, 118.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700522 sequences (100000091 bp) in 2.404 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292056, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 176, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324)
[M::mem_pestat] mean and std.dev: (182.10, 69.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 461)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (244.17, 119.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701332 reads in 134.464 CPU sec, 4.275 real sec
[M::process] read 699504 sequences (100000040 bp) in 1.600 CPU sec, 1.623 real sec...
[M::mem_process_seqs] Processed 701532 reads in 86.088 CPU sec, 2.713 real sec
sorting block 71, #reads = 1093265 mem = 536879799 peak = 1098317824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291891, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (247.01, 120.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699186 sequences (100000154 bp) in 2.276 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291736, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.76, 122.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700522 reads in 134.010 CPU sec, 4.265 real sec
[M::process] read 698394 sequences (100000247 bp) in 1.597 CPU sec, 1.619 real sec...
[M::mem_process_seqs] Processed 699504 reads in 85.402 CPU sec, 2.693 real sec
sorting block 72, #reads = 1093254 mem = 536881247 peak = 1098317824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291182, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (307.67, 125.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.58, 121.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698706 sequences (100000089 bp) in 2.329 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291053, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.15, 120.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699186 reads in 136.184 CPU sec, 4.326 real sec
[M::process] read 698866 sequences (100000097 bp) in 1.440 CPU sec, 1.445 real sec...
sorting block 73, #reads = 1092610 mem = 536879488 peak = 1098317824
[M::mem_process_seqs] Processed 698394 reads in 86.993 CPU sec, 2.747 real sec
sorting block 74, #reads = 1091466 mem = 536884028 peak = 1108516864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291521, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.50, 120.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698696 sequences (100000286 bp) in 2.427 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291229, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.80, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698706 reads in 138.114 CPU sec, 4.384 real sec
[M::process] read 698368 sequences (100000181 bp) in 1.480 CPU sec, 1.474 real sec...
[M::mem_process_seqs] Processed 698866 reads in 87.668 CPU sec, 2.769 real sec
sorting block 75, #reads = 1091491 mem = 536879182 peak = 1108516864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290900, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.22, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701366 sequences (100000122 bp) in 2.384 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290672, 8, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (363, 389, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (155, 675)
[M::mem_pestat] mean and std.dev: (365.57, 89.73)
[M::mem_pestat] low and high boundaries for proper pairs: (7, 779)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.88, 119.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 709706 sequences (100000032 bp) in 1.490 CPU sec, 1.494 real sec...
[M::mem_process_seqs] Processed 698696 reads in 139.652 CPU sec, 4.437 real sec
[M::mem_process_seqs] Processed 698368 reads in 88.833 CPU sec, 2.817 real sec
sorting block 76, #reads = 1090925 mem = 536878166 peak = 1108516864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291409, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 194, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (237.75, 111.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.26, 116.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 460, 787)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1981)
[M::mem_pestat] mean and std.dev: (398.11, 256.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2578)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 708714 sequences (100000038 bp) in 2.474 CPU sec, 2.571 real sec...
[M::process] read 706268 sequences (100000265 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 294952, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 199, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (221.32, 103.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (407, 1656, 1656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4154)
[M::mem_pestat] mean and std.dev: (1119.22, 982.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5403)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701366 reads in 155.120 CPU sec, 4.929 real sec
sorting block 77, #reads = 1088365 mem = 536880401 peak = 1108516864
[M::mem_process_seqs] Processed 709706 reads in 96.116 CPU sec, 3.046 real sec
sorting block 78, #reads = 1086930 mem = 536878942 peak = 1108516864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 294274, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.17, 105.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 191, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (182.17, 70.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RR
[M::process] read 704530 sequences (100000103 bp) in 2.487 CPU sec, 2.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293610, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 334, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (225.75, 84.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (230.80, 110.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703092 sequences (100000094 bp) in 1.490 CPU sec, 1.494 real sec...
[M::mem_process_seqs] Processed 708714 reads in 149.233 CPU sec, 4.737 real sec
[M::mem_process_seqs] Processed 706268 reads in 93.288 CPU sec, 2.959 real sec
sorting block 79, #reads = 1090770 mem = 536880048 peak = 1108516864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292871, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (234.86, 112.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702318 sequences (100000190 bp) in 2.417 CPU sec, 2.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292273, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (239.16, 115.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701198 sequences (100000136 bp) in 1.490 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 704530 reads in 141.751 CPU sec, 4.499 real sec
[M::mem_process_seqs] Processed 703092 reads in 89.062 CPU sec, 2.814 real sec
sorting block 80, #reads = 1092346 mem = 536879641 peak = 1108516864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292101, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.66, 116.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700956 sequences (100000136 bp) in 2.485 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291761, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 327, 716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1778)
[M::mem_pestat] mean and std.dev: (350.50, 228.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2309)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.65, 119.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 293, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (235.43, 80.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 700298 sequences (100000209 bp) in 1.460 CPU sec, 1.464 real sec...
[M::mem_process_seqs] Processed 702318 reads in 141.868 CPU sec, 4.502 real sec
[M::mem_process_seqs] Processed 701198 reads in 89.840 CPU sec, 2.843 real sec
sorting block 81, #reads = 1091625 mem = 536878751 peak = 1108516864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292253, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.90, 119.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 314, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (252.09, 95.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RR
sorting block 82, #reads = 1091050 mem = 536879476 peak = 1125773312
[M::process] read 699934 sequences (100000285 bp) in 2.311 CPU sec, 2.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291686, 6, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 244, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441)
[M::mem_pestat] mean and std.dev: (336.58, 233.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1857)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (245.02, 118.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 173, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (192.67, 94.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 700434 sequences (100000289 bp) in 1.470 CPU sec, 1.478 real sec...
[M::mem_process_seqs] Processed 700956 reads in 140.688 CPU sec, 4.466 real sec
[M::mem_process_seqs] Processed 700298 reads in 88.850 CPU sec, 2.811 real sec
sorting block 83, #reads = 1090155 mem = 536877095 peak = 1125773312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291214, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (245.07, 118.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699356 sequences (100000042 bp) in 2.512 CPU sec, 2.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291580, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (243.56, 117.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699934 reads in 139.090 CPU sec, 4.415 real sec
[M::process] read 699280 sequences (100000112 bp) in 1.536 CPU sec, 1.540 real sec...
[M::mem_process_seqs] Processed 700434 reads in 88.433 CPU sec, 2.790 real sec
sorting block 84, #reads = 1090724 mem = 536878645 peak = 1125773312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291132, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.23, 118.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 197, 935)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2471)
[M::mem_pestat] mean and std.dev: (266.33, 242.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3239)
[M::mem_pestat] skip orientation RR
[M::process] read 698986 sequences (100000004 bp) in 2.488 CPU sec, 2.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291689, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (276, 707, 2130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5838)
[M::mem_pestat] mean and std.dev: (1259.25, 1543.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7692)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (247.06, 118.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699356 reads in 136.273 CPU sec, 4.325 real sec
[M::process] read 698468 sequences (100000240 bp) in 1.498 CPU sec, 1.495 real sec...
[M::mem_process_seqs] Processed 699280 reads in 86.325 CPU sec, 2.725 real sec
sorting block 85, #reads = 1091475 mem = 536879778 peak = 1125773312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290988, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.81, 119.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 653242 sequences (93541135 bp) in 2.425 CPU sec, 2.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290728, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.75, 119.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698986 reads in 132.595 CPU sec, 4.253 real sec
sorting block 86, #reads = 1091837 mem = 536877437 peak = 1125773312
[M::mem_process_seqs] Processed 698468 reads in 83.557 CPU sec, 2.684 real sec
sorting block 87, #reads = 1092808 mem = 536879324 peak = 1221144576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 272230, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.35, 119.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 653242 reads in 70.046 CPU sec, 2.334 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96539944
overall: 70041395200 mem 11401.053 user 406.053 sys 1356.580 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1356.620 sec; CPU: 11807.109 sec
sorting block 88, #reads = 842635 mem = 414505847 peak = 1221144576
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5182016170
output reads: 97032518
bam_mem_sort: 89 calls 31.082 user 0.478 sys 31.773 real
bam_write: 89 calls 61.404 user 1.768 sys 63.608 real
execute: 1 calls 18.298 user 1.307 sys 1383.995 real
merge_files: 1 calls 18.282 user 1.283 sys 26.275 real
parse_chunk: 1421 calls 45.474 user 11.032 sys 57.475 real
read_chunk: 3837 calls 37.822 user 35.163 sys 335.171 real
sort_block: 1 calls 30.182 user 34.506 sys 323.997 real
write_chunk: 2414 calls 17.937 user 1.079 sys 25.486 real
overall: 3025780736 mem 1027.900 user 56.205 sys 1384.026 real
ls: cannot access '*.cram': No such file or directory