.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L004;0004)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e2/56195f39150905bf56c360059cd724/.command.err
Size: 134.4 KB
Task: DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L004;0004)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1648.799 CPU sec, 174.556 real sec
[M::process] read 702700 sequences (100000085 bp) in 0.962 CPU sec, 0.971 real sec...
[M::process] read 702146 sequences (100000201 bp) in 1.269 CPU sec, 1.270 real sec...
[M::process] read 701708 sequences (100000256 bp) in 1.288 CPU sec, 1.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292060, 8, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.65, 114.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 283, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1387)
[M::mem_pestat] mean and std.dev: (381.69, 246.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1778)
[M::mem_pestat] skip orientation RR
[M::process] read 700804 sequences (100000121 bp) in 1.219 CPU sec, 1.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292098, 7, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 262, 854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146)
[M::mem_pestat] mean and std.dev: (394.50, 326.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2792)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.79, 115.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 265, 594)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362)
[M::mem_pestat] mean and std.dev: (253.44, 137.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1746)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291758, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.03, 116.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702700 reads in 220.464 CPU sec, 7.047 real sec
[M::mem_process_seqs] Processed 702146 reads in 144.239 CPU sec, 4.721 real sec
[M::mem_process_seqs] Processed 701708 reads in 157.938 CPU sec, 5.221 real sec
sorting block 0, #reads = 1096291 mem = 536881175 peak = 590798848
[M::process] read 699962 sequences (100000143 bp) in 1.953 CPU sec, 2.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291653, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.34, 117.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699414 sequences (100000253 bp) in 1.319 CPU sec, 1.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291467, 14, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.56, 119.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 109, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (83.09, 52.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 651)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 700804 reads in 132.712 CPU sec, 4.231 real sec
sorting block 1, #reads = 1095190 mem = 536879188 peak = 776622080
[M::process] read 698930 sequences (100000162 bp) in 1.357 CPU sec, 1.378 real sec...
[M::mem_process_seqs] Processed 699962 reads in 82.566 CPU sec, 2.603 real sec
sorting block 2, #reads = 1095238 mem = 536880563 peak = 889462784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291246, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.58, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698614 sequences (100000234 bp) in 1.834 CPU sec, 1.921 real sec...
[M::process] read 698546 sequences (100000144 bp) in 1.300 CPU sec, 1.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291102, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.78, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699414 reads in 132.318 CPU sec, 4.220 real sec
[M::mem_process_seqs] Processed 698930 reads in 81.929 CPU sec, 2.614 real sec
sorting block 3, #reads = 1094422 mem = 536879297 peak = 889462784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291276, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.60, 120.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 662, 1666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4516)
[M::mem_pestat] mean and std.dev: (840.00, 622.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5941)
[M::mem_pestat] skip orientation RR
[M::process] read 698270 sequences (100000115 bp) in 1.836 CPU sec, 1.947 real sec...
[M::process] read 698496 sequences (100000071 bp) in 1.310 CPU sec, 1.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290981, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 204, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143)
[M::mem_pestat] mean and std.dev: (283.78, 207.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1472)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.22, 120.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 344, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (294.64, 110.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698614 reads in 133.126 CPU sec, 4.240 real sec
[M::mem_process_seqs] Processed 698546 reads in 82.680 CPU sec, 2.644 real sec
sorting block 4, #reads = 1094452 mem = 536878143 peak = 889462784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291059, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.45, 119.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698542 sequences (100000090 bp) in 2.070 CPU sec, 2.211 real sec...
[M::process] read 697420 sequences (100000296 bp) in 1.330 CPU sec, 1.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291045, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.75, 118.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 209, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (232.56, 98.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698270 reads in 134.474 CPU sec, 4.286 real sec
sorting block 5, #reads = 1094071 mem = 536877295 peak = 889462784
[M::mem_process_seqs] Processed 698496 reads in 83.727 CPU sec, 2.669 real sec
sorting block 6, #reads = 1094199 mem = 536880596 peak = 889462784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290389, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.96, 119.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697118 sequences (100000047 bp) in 2.123 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290913, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (251.92, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696696 sequences (100000291 bp) in 1.370 CPU sec, 1.375 real sec...
[M::mem_process_seqs] Processed 698542 reads in 134.983 CPU sec, 4.303 real sec
[M::process] read 696092 sequences (100000086 bp) in 1.515 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290539, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.92, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697118 reads in 77.873 CPU sec, 2.661 real sec
sorting block 7, #reads = 1093312 mem = 536878738 peak = 889462784
[M::mem_process_seqs] Processed 697420 reads in 180.126 CPU sec, 5.921 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290303, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 307, 1291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3527)
[M::mem_pestat] mean and std.dev: (594.47, 706.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4645)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.18, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 643, 1051)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2729)
[M::mem_pestat] mean and std.dev: (732.71, 553.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3568)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 695814 sequences (100000225 bp) in 2.133 CPU sec, 2.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290555, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (254.98, 121.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696696 reads in 145.771 CPU sec, 4.652 real sec
sorting block 8, #reads = 1092458 mem = 536891406 peak = 889462784
[M::process] read 695904 sequences (100000186 bp) in 1.528 CPU sec, 1.552 real sec...
[M::mem_process_seqs] Processed 696092 reads in 84.703 CPU sec, 2.682 real sec
sorting block 9, #reads = 1092589 mem = 536883986 peak = 889462784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289970, 10, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 208, 723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1903)
[M::mem_pestat] mean and std.dev: (287.50, 253.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.74, 122.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (92, 600, 880)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2456)
[M::mem_pestat] mean and std.dev: (555.78, 639.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3244)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (297, 605, 2921)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8169)
[M::mem_pestat] mean and std.dev: (1580.93, 2009.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10793)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 703586 sequences (100000109 bp) in 2.231 CPU sec, 2.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290526, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.87, 122.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702586 sequences (100000066 bp) in 1.400 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 695814 reads in 135.051 CPU sec, 4.310 real sec
[M::mem_process_seqs] Processed 695904 reads in 85.355 CPU sec, 2.709 real sec
sorting block 10, #reads = 1092755 mem = 536876142 peak = 889462784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292611, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 556, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1705)
[M::mem_pestat] mean and std.dev: (404.56, 264.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2217)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (240.27, 116.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701952 sequences (100000130 bp) in 2.340 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292869, 6, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (242.45, 117.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 273, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233)
[M::mem_pestat] mean and std.dev: (304.00, 148.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1577)
[M::mem_pestat] skip orientation RR
[M::process] read 701410 sequences (100000126 bp) in 1.440 CPU sec, 1.441 real sec...
[M::mem_process_seqs] Processed 703586 reads in 135.473 CPU sec, 4.320 real sec
[M::mem_process_seqs] Processed 702586 reads in 84.204 CPU sec, 2.672 real sec
sorting block 11, #reads = 1094791 mem = 536878773 peak = 889462784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292212, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (264, 265, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (294.70, 141.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (243.89, 118.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700532 sequences (100000023 bp) in 2.371 CPU sec, 2.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291912, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 346, 1102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2844)
[M::mem_pestat] mean and std.dev: (549.92, 450.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3715)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.46, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700194 sequences (100000255 bp) in 1.450 CPU sec, 1.450 real sec...
[M::mem_process_seqs] Processed 701952 reads in 136.521 CPU sec, 4.349 real sec
sorting block 12, #reads = 1096779 mem = 536877727 peak = 889462784
[M::mem_process_seqs] Processed 701410 reads in 85.499 CPU sec, 2.715 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291457, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (247.92, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1097455 mem = 536878694 peak = 916656128
[M::process] read 699534 sequences (100000008 bp) in 2.408 CPU sec, 2.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291872, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.42, 121.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700532 reads in 136.893 CPU sec, 4.372 real sec
[M::process] read 699048 sequences (100000075 bp) in 1.587 CPU sec, 1.607 real sec...
[M::mem_process_seqs] Processed 700194 reads in 85.127 CPU sec, 2.690 real sec
sorting block 14, #reads = 1096447 mem = 536880401 peak = 916656128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291190, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 166, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (194.55, 76.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.55, 121.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699104 sequences (100000028 bp) in 2.349 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291500, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 357, 1703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4719)
[M::mem_pestat] mean and std.dev: (691.75, 723.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6227)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.48, 122.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699534 reads in 130.486 CPU sec, 4.160 real sec
[M::mem_process_seqs] Processed 699048 reads in 83.376 CPU sec, 2.632 real sec
[M::process] read 698580 sequences (100000147 bp) in 1.704 CPU sec, 1.742 real sec...
sorting block 15, #reads = 1094754 mem = 536880053 peak = 916656128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291399, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.18, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (395, 452, 1375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3335)
[M::mem_pestat] mean and std.dev: (538.46, 431.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4315)
[M::mem_pestat] skip orientation RR
[M::process] read 698942 sequences (100000074 bp) in 2.306 CPU sec, 2.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290788, 12, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.13, 119.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (97, 249, 710)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1936)
[M::mem_pestat] mean and std.dev: (454.33, 499.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2549)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 269, 1346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3706)
[M::mem_pestat] mean and std.dev: (478.67, 482.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4886)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699104 reads in 131.668 CPU sec, 4.189 real sec
[M::mem_process_seqs] Processed 698580 reads in 83.470 CPU sec, 2.632 real sec
[M::process] read 698668 sequences (100000095 bp) in 1.654 CPU sec, 1.676 real sec...
sorting block 16, #reads = 1094849 mem = 536880848 peak = 916656128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291206, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.64, 118.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698004 sequences (100000012 bp) in 2.354 CPU sec, 2.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290735, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (252, 317, 3019)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8553)
[M::mem_pestat] mean and std.dev: (1313.91, 1716.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11320)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.39, 118.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698942 reads in 131.755 CPU sec, 4.189 real sec
sorting block 17, #reads = 1093703 mem = 536879196 peak = 916656128
[M::mem_process_seqs] Processed 698668 reads in 83.635 CPU sec, 2.637 real sec
[M::process] read 697438 sequences (100000107 bp) in 1.607 CPU sec, 1.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290495, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 382)
[M::mem_pestat] mean and std.dev: (184.58, 60.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 455)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (249.00, 118.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 18, #reads = 1094090 mem = 536881399 peak = 988688384
[M::process] read 697714 sequences (100000191 bp) in 2.369 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290557, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 265, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (255.67, 99.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.10, 118.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698004 reads in 131.628 CPU sec, 4.187 real sec
[M::mem_process_seqs] Processed 697438 reads in 82.902 CPU sec, 2.620 real sec
[M::process] read 696802 sequences (100000100 bp) in 1.632 CPU sec, 1.662 real sec...
sorting block 19, #reads = 1092340 mem = 536880754 peak = 988688384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291277, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (251.84, 120.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697090 sequences (100000141 bp) in 2.336 CPU sec, 2.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290485, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.61, 121.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697714 reads in 131.284 CPU sec, 4.173 real sec
[M::mem_process_seqs] Processed 696802 reads in 82.644 CPU sec, 2.603 real sec
[M::process] read 699050 sequences (100000222 bp) in 1.617 CPU sec, 1.644 real sec...
sorting block 20, #reads = 1093604 mem = 536879490 peak = 988688384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290467, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (253.05, 121.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704008 sequences (100000106 bp) in 2.367 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291012, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.95, 120.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697090 reads in 132.548 CPU sec, 4.216 real sec
[M::process] read 703602 sequences (100000028 bp) in 1.594 CPU sec, 1.608 real sec...
[M::mem_process_seqs] Processed 699050 reads in 84.758 CPU sec, 2.672 real sec
sorting block 21, #reads = 1092735 mem = 536876226 peak = 988688384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292355, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 248, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172)
[M::mem_pestat] mean and std.dev: (307.42, 164.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1497)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.73, 115.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 22, #reads = 1092015 mem = 536883017 peak = 988688384
[M::process] read 702474 sequences (100000018 bp) in 2.357 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292774, 6, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (239.99, 115.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 385, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (327.73, 125.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 704008 reads in 131.142 CPU sec, 4.167 real sec
[M::process] read 701532 sequences (100000050 bp) in 1.630 CPU sec, 1.645 real sec...
[M::mem_process_seqs] Processed 703602 reads in 83.660 CPU sec, 2.633 real sec
sorting block 23, #reads = 1097387 mem = 536886588 peak = 988688384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292527, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.71, 116.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700292 sequences (100000142 bp) in 2.430 CPU sec, 2.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291911, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 355, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559)
[M::mem_pestat] mean and std.dev: (365.09, 196.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2019)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.14, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702474 reads in 131.219 CPU sec, 4.175 real sec
[M::mem_process_seqs] Processed 701532 reads in 83.652 CPU sec, 2.638 real sec
[M::process] read 700036 sequences (100000148 bp) in 1.719 CPU sec, 1.749 real sec...
sorting block 24, #reads = 1095948 mem = 536881834 peak = 988688384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291909, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.78, 119.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699474 sequences (100000221 bp) in 2.107 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291412, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.87, 119.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700292 reads in 129.576 CPU sec, 4.129 real sec
[M::mem_process_seqs] Processed 700036 reads in 81.963 CPU sec, 2.586 real sec
[M::process] read 699174 sequences (100000006 bp) in 1.715 CPU sec, 1.760 real sec...
sorting block 25, #reads = 1096082 mem = 536878203 peak = 988688384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291123, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.13, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699018 sequences (100000083 bp) in 2.345 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291795, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.80, 120.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699474 reads in 132.598 CPU sec, 4.220 real sec
sorting block 26, #reads = 1095291 mem = 536879982 peak = 988688384
[M::mem_process_seqs] Processed 699174 reads in 83.696 CPU sec, 2.636 real sec
[M::process] read 698488 sequences (100000081 bp) in 1.701 CPU sec, 1.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291130, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.94, 120.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1094651 mem = 536878572 peak = 988688384
[M::process] read 698306 sequences (100000121 bp) in 2.370 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291080, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.66, 118.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699018 reads in 132.849 CPU sec, 4.227 real sec
[M::mem_process_seqs] Processed 698488 reads in 84.420 CPU sec, 2.663 real sec
[M::process] read 698108 sequences (100000029 bp) in 1.706 CPU sec, 1.749 real sec...
sorting block 28, #reads = 1095464 mem = 536878027 peak = 988688384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290694, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 191, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110)
[M::mem_pestat] mean and std.dev: (337.73, 287.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1486)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.12, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697572 sequences (100000143 bp) in 2.167 CPU sec, 2.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291217, 8, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 204, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (144, 244)
[M::mem_pestat] mean and std.dev: (198.33, 8.20)
[M::mem_pestat] low and high boundaries for proper pairs: (124, 264)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.57, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698306 reads in 132.261 CPU sec, 4.217 real sec
[M::mem_process_seqs] Processed 698108 reads in 83.692 CPU sec, 2.637 real sec
[M::process] read 697286 sequences (100000283 bp) in 1.702 CPU sec, 1.738 real sec...
sorting block 29, #reads = 1092769 mem = 536876713 peak = 988688384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290844, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.05, 119.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697772 sequences (100000116 bp) in 2.339 CPU sec, 2.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290785, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.68, 119.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697572 reads in 133.371 CPU sec, 4.241 real sec
[M::mem_process_seqs] Processed 697286 reads in 84.331 CPU sec, 2.651 real sec
[M::process] read 697860 sequences (100000022 bp) in 1.638 CPU sec, 1.658 real sec...
sorting block 30, #reads = 1093377 mem = 536878476 peak = 988688384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290594, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 386, 1078)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2748)
[M::mem_pestat] mean and std.dev: (608.06, 638.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3583)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.89, 119.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 31, #reads = 1091960 mem = 536883315 peak = 998526976
[M::process] read 697372 sequences (100000058 bp) in 2.106 CPU sec, 2.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291080, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.97, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697772 reads in 133.759 CPU sec, 4.260 real sec
[M::process] read 701520 sequences (100000041 bp) in 1.565 CPU sec, 1.580 real sec...
[M::mem_process_seqs] Processed 697860 reads in 84.575 CPU sec, 2.664 real sec
sorting block 32, #reads = 1091255 mem = 536880605 peak = 998526976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290481, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.70, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 212, 1145)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3153)
[M::mem_pestat] mean and std.dev: (575.67, 709.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4157)
[M::mem_pestat] skip orientation RR
[M::process] read 703212 sequences (100000152 bp) in 2.358 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292154, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 181, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (195.18, 51.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 575)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.70, 116.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697372 reads in 134.906 CPU sec, 4.287 real sec
[M::process] read 702496 sequences (100000058 bp) in 1.484 CPU sec, 1.491 real sec...
[M::mem_process_seqs] Processed 701520 reads in 85.620 CPU sec, 2.703 real sec
sorting block 33, #reads = 1092479 mem = 536881399 peak = 998526976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292359, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 193, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (213.11, 58.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (241.88, 116.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702034 sequences (100000138 bp) in 2.337 CPU sec, 2.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292354, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.42, 116.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 274, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077)
[M::mem_pestat] mean and std.dev: (290.45, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1372)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 703212 reads in 131.652 CPU sec, 4.184 real sec
[M::process] read 701270 sequences (100000016 bp) in 1.546 CPU sec, 1.551 real sec...
[M::mem_process_seqs] Processed 702496 reads in 83.103 CPU sec, 2.618 real sec
sorting block 34, #reads = 1095474 mem = 536880296 peak = 998526976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292318, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (244.60, 118.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700270 sequences (100000227 bp) in 2.135 CPU sec, 2.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292130, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (246.42, 118.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702034 reads in 132.211 CPU sec, 4.215 real sec
sorting block 35, #reads = 1097015 mem = 536880128 peak = 998526976
[M::mem_process_seqs] Processed 701270 reads in 83.797 CPU sec, 2.637 real sec
[M::process] read 700000 sequences (100000156 bp) in 1.630 CPU sec, 1.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292113, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.45, 121.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1097183 mem = 536879987 peak = 998526976
[M::process] read 699464 sequences (100000182 bp) in 2.159 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291476, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.63, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700270 reads in 131.799 CPU sec, 4.200 real sec
[M::mem_process_seqs] Processed 700000 reads in 83.522 CPU sec, 2.630 real sec
[M::process] read 699426 sequences (100000076 bp) in 1.648 CPU sec, 1.674 real sec...
sorting block 37, #reads = 1095708 mem = 536877092 peak = 998526976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291250, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 359, 1317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3521)
[M::mem_pestat] mean and std.dev: (765.09, 849.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4623)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (250.89, 121.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700304 sequences (100000215 bp) in 2.410 CPU sec, 2.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 291179, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 317, 1313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3549)
[M::mem_pestat] mean and std.dev: (836.19, 1073.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5129)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.85, 121.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699464 reads in 134.051 CPU sec, 4.263 real sec
[M::mem_process_seqs] Processed 699426 reads in 84.755 CPU sec, 2.670 real sec
[M::process] read 699090 sequences (100000143 bp) in 1.607 CPU sec, 1.635 real sec...
sorting block 38, #reads = 1094744 mem = 536879306 peak = 998526976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291704, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 258, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (227.73, 80.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.03, 117.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698848 sequences (100000002 bp) in 2.212 CPU sec, 2.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291092, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (246.94, 117.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (328, 586, 1341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3367)
[M::mem_pestat] mean and std.dev: (778.85, 661.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4380)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700304 reads in 133.715 CPU sec, 4.259 real sec
[M::process] read 698120 sequences (100000271 bp) in 1.626 CPU sec, 1.651 real sec...
sorting block 39, #reads = 1094411 mem = 536878799 peak = 998526976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290855, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.05, 118.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 201, 702)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1818)
[M::mem_pestat] mean and std.dev: (403.50, 303.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2376)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698848 reads in 73.313 CPU sec, 2.318 real sec
[M::process] read 698010 sequences (100000200 bp) in 1.816 CPU sec, 1.827 real sec...
sorting block 40, #reads = 1094514 mem = 536881497 peak = 999641088
[M::mem_process_seqs] Processed 699090 reads in 180.814 CPU sec, 5.720 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290958, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (247.91, 116.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697374 sequences (100000199 bp) in 2.549 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290287, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (247.91, 116.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698120 reads in 145.250 CPU sec, 4.606 real sec
[M::mem_process_seqs] Processed 698010 reads in 85.160 CPU sec, 2.687 real sec
sorting block 41, #reads = 1092779 mem = 536882256 peak = 999641088
[M::process] read 697448 sequences (100000297 bp) in 1.939 CPU sec, 2.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290445, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (248.52, 116.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1092416 mem = 536879643 peak = 999641088
[M::process] read 697380 sequences (100000272 bp) in 1.968 CPU sec, 2.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290590, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.94, 119.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697374 reads in 132.994 CPU sec, 4.238 real sec
[M::mem_process_seqs] Processed 697448 reads in 84.246 CPU sec, 2.654 real sec
[M::process] read 697076 sequences (100000072 bp) in 1.677 CPU sec, 1.710 real sec...
sorting block 43, #reads = 1091933 mem = 536878119 peak = 999641088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291161, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 267, 3418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9804)
[M::mem_pestat] mean and std.dev: (1052.83, 1370.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12997)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.04, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 708302 sequences (100000192 bp) in 2.204 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290419, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.64, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697380 reads in 133.222 CPU sec, 4.242 real sec
[M::mem_process_seqs] Processed 697076 reads in 84.792 CPU sec, 2.672 real sec
[M::process] read 706208 sequences (100000180 bp) in 1.894 CPU sec, 1.947 real sec...
sorting block 44, #reads = 1092390 mem = 536887656 peak = 999641088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 294440, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (223.65, 105.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 562, 972)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2522)
[M::mem_pestat] mean and std.dev: (524.55, 391.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3297)
[M::mem_pestat] skip orientation RR
[M::process] read 703986 sequences (100000002 bp) in 2.079 CPU sec, 2.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293817, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.70, 110.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 708302 reads in 139.907 CPU sec, 4.457 real sec
[M::process] read 702878 sequences (100000140 bp) in 1.602 CPU sec, 1.620 real sec...
[M::mem_process_seqs] Processed 706208 reads in 87.566 CPU sec, 2.758 real sec
sorting block 45, #reads = 1092401 mem = 536876294 peak = 999641088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292517, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (236.97, 114.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702070 sequences (100000008 bp) in 2.366 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 292662, 7, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 838, 1887)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5233)
[M::mem_pestat] mean and std.dev: (951.94, 802.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6906)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.76, 116.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (314, 566, 1705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4487)
[M::mem_pestat] mean and std.dev: (842.00, 967.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5878)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 703986 reads in 134.017 CPU sec, 4.259 real sec
[M::process] read 701206 sequences (100000204 bp) in 1.464 CPU sec, 1.467 real sec...
sorting block 46, #reads = 1096265 mem = 536881493 peak = 999641088
[M::mem_process_seqs] Processed 702878 reads in 84.707 CPU sec, 2.679 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291993, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (242.10, 117.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1095237 mem = 536877520 peak = 1011552256
[M::process] read 700942 sequences (100000087 bp) in 2.039 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291522, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.38, 118.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702070 reads in 133.805 CPU sec, 4.258 real sec
[M::process] read 700012 sequences (100000262 bp) in 1.430 CPU sec, 1.424 real sec...
[M::mem_process_seqs] Processed 701206 reads in 84.112 CPU sec, 2.656 real sec
sorting block 48, #reads = 1094531 mem = 536880897 peak = 1011552256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292284, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.72, 120.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699744 sequences (100000132 bp) in 2.331 CPU sec, 2.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 291591, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.71, 120.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698912 sequences (100000263 bp) in 1.440 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 700942 reads in 134.311 CPU sec, 4.261 real sec
[M::mem_process_seqs] Processed 700012 reads in 85.186 CPU sec, 2.688 real sec
sorting block 49, #reads = 1095079 mem = 536879952 peak = 1011552256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291694, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.40, 119.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699194 sequences (100000200 bp) in 2.392 CPU sec, 2.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290873, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.48, 119.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698448 sequences (100000170 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 699744 reads in 135.296 CPU sec, 4.291 real sec
sorting block 50, #reads = 1093235 mem = 536877530 peak = 1011552256
[M::mem_process_seqs] Processed 698912 reads in 85.603 CPU sec, 2.702 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291432, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.79, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1093578 mem = 536880508 peak = 1011552256
[M::process] read 698900 sequences (100000217 bp) in 2.396 CPU sec, 2.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290720, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.94, 119.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698298 sequences (100000200 bp) in 1.450 CPU sec, 1.449 real sec...
[M::mem_process_seqs] Processed 699194 reads in 136.405 CPU sec, 4.326 real sec
[M::mem_process_seqs] Processed 698448 reads in 86.082 CPU sec, 2.717 real sec
sorting block 52, #reads = 1090991 mem = 536878207 peak = 1011552256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290991, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.70, 118.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697904 sequences (100000005 bp) in 2.384 CPU sec, 2.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291057, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.08, 119.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697936 sequences (100000008 bp) in 1.440 CPU sec, 1.439 real sec...
[M::mem_process_seqs] Processed 698900 reads in 136.588 CPU sec, 4.333 real sec
[M::mem_process_seqs] Processed 698298 reads in 86.283 CPU sec, 2.725 real sec
sorting block 53, #reads = 1090880 mem = 536883794 peak = 1011552256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290827, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.92, 119.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698752 sequences (100000283 bp) in 2.428 CPU sec, 2.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290791, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (381, 807, 2525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6813)
[M::mem_pestat] mean and std.dev: (1097.60, 956.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8957)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.39, 120.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 710502 sequences (100000097 bp) in 1.460 CPU sec, 1.466 real sec...
[M::mem_process_seqs] Processed 697904 reads in 136.663 CPU sec, 4.334 real sec
[M::mem_process_seqs] Processed 697936 reads in 86.904 CPU sec, 2.743 real sec
sorting block 54, #reads = 1091683 mem = 536883052 peak = 1011552256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291036, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.25, 120.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 709322 sequences (100000301 bp) in 2.426 CPU sec, 2.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 294953, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 197, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (219.11, 102.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706988 sequences (100000262 bp) in 1.480 CPU sec, 1.477 real sec...
[M::mem_process_seqs] Processed 698752 reads in 140.872 CPU sec, 4.469 real sec
sorting block 55, #reads = 1089949 mem = 536878531 peak = 1011552256
[M::mem_process_seqs] Processed 710502 reads in 89.562 CPU sec, 2.831 real sec
sorting block 56, #reads = 1093867 mem = 536878602 peak = 1060605952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 294995, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 200, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (222.09, 104.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 213, 1136)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3138)
[M::mem_pestat] mean and std.dev: (549.69, 627.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4139)
[M::mem_pestat] skip orientation RR
[M::process] read 705488 sequences (100000154 bp) in 2.404 CPU sec, 2.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293568, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.33, 108.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705290 sequences (100000041 bp) in 1.470 CPU sec, 1.468 real sec...
[M::mem_process_seqs] Processed 709322 reads in 145.407 CPU sec, 4.616 real sec
[M::mem_process_seqs] Processed 706988 reads in 90.597 CPU sec, 2.867 real sec
sorting block 57, #reads = 1093866 mem = 536878741 peak = 1060605952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 294057, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 208, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (232.04, 110.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704398 sequences (100000108 bp) in 2.416 CPU sec, 2.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 293627, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 211, 735)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1929)
[M::mem_pestat] mean and std.dev: (371.38, 313.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2526)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.98, 112.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703816 sequences (100000293 bp) in 1.470 CPU sec, 1.473 real sec...
[M::mem_process_seqs] Processed 705488 reads in 141.755 CPU sec, 4.501 real sec
[M::mem_process_seqs] Processed 705290 reads in 89.331 CPU sec, 2.827 real sec
sorting block 58, #reads = 1093941 mem = 536878076 peak = 1060605952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293137, 1, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (236.86, 114.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (288, 370, 3852)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10980)
[M::mem_pestat] mean and std.dev: (1408.00, 1764.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14544)
[M::mem_pestat] skip orientation RR
[M::process] read 702682 sequences (100000185 bp) in 2.468 CPU sec, 2.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293150, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.92, 115.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701072 sequences (100000227 bp) in 1.470 CPU sec, 1.469 real sec...
[M::mem_process_seqs] Processed 704398 reads in 138.422 CPU sec, 4.390 real sec
sorting block 59, #reads = 1094469 mem = 536881779 peak = 1060605952
[M::mem_process_seqs] Processed 703816 reads in 87.032 CPU sec, 2.748 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292467, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (264, 353, 1032)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2568)
[M::mem_pestat] mean and std.dev: (566.36, 378.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3336)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (242.47, 118.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 60, #reads = 1093465 mem = 536879906 peak = 1060605952
[M::process] read 700042 sequences (100000285 bp) in 2.212 CPU sec, 2.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292107, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.40, 118.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699744 sequences (100000133 bp) in 1.460 CPU sec, 1.453 real sec...
[M::mem_process_seqs] Processed 702682 reads in 139.232 CPU sec, 4.426 real sec
[M::mem_process_seqs] Processed 701072 reads in 88.437 CPU sec, 2.803 real sec
sorting block 61, #reads = 1092851 mem = 536879823 peak = 1060605952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291733, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.52, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 227, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1422)
[M::mem_pestat] mean and std.dev: (245.00, 170.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1846)
[M::mem_pestat] skip orientation RR
[M::process] read 699324 sequences (100000176 bp) in 2.425 CPU sec, 2.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291861, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 283, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261)
[M::mem_pestat] mean and std.dev: (373.11, 304.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1614)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.21, 119.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698942 sequences (100000069 bp) in 1.450 CPU sec, 1.447 real sec...
[M::mem_process_seqs] Processed 700042 reads in 137.694 CPU sec, 4.369 real sec
[M::mem_process_seqs] Processed 699744 reads in 87.108 CPU sec, 2.753 real sec
sorting block 62, #reads = 1091633 mem = 536878334 peak = 1060605952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291299, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.67, 119.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 338, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (334.78, 201.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RR
[M::process] read 698062 sequences (100000127 bp) in 2.425 CPU sec, 2.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290591, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.60, 118.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697978 sequences (100000161 bp) in 1.440 CPU sec, 1.436 real sec...
[M::mem_process_seqs] Processed 699324 reads in 136.805 CPU sec, 4.341 real sec
[M::mem_process_seqs] Processed 698942 reads in 86.938 CPU sec, 2.745 real sec
sorting block 63, #reads = 1092166 mem = 536880223 peak = 1060605952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291052, 7, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 194, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299)
[M::mem_pestat] mean and std.dev: (294.33, 313.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1693)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.01, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698702 sequences (100000144 bp) in 2.417 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290899, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 348, 4484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13096)
[M::mem_pestat] mean and std.dev: (1529.92, 2217.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17402)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.35, 120.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698062 reads in 133.690 CPU sec, 4.241 real sec
[M::process] read 707002 sequences (100000189 bp) in 1.489 CPU sec, 1.489 real sec...
sorting block 64, #reads = 1090763 mem = 536880070 peak = 1060605952
[M::mem_process_seqs] Processed 697978 reads in 84.548 CPU sec, 2.670 real sec
sorting block 65, #reads = 1091834 mem = 536878469 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290998, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.27, 119.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 707360 sequences (100000002 bp) in 2.418 CPU sec, 2.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 293458, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 246, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (272.94, 133.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.15, 108.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 705938 sequences (100000258 bp) in 1.420 CPU sec, 1.423 real sec...
[M::mem_process_seqs] Processed 698702 reads in 141.494 CPU sec, 4.492 real sec
[M::mem_process_seqs] Processed 707002 reads in 90.788 CPU sec, 2.873 real sec
sorting block 66, #reads = 1088089 mem = 536881252 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293972, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (228.35, 108.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704528 sequences (100000111 bp) in 2.425 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293436, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 208, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (232.49, 111.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707360 reads in 132.201 CPU sec, 4.194 real sec
[M::process] read 703462 sequences (100000050 bp) in 1.469 CPU sec, 1.467 real sec...
[M::mem_process_seqs] Processed 705938 reads in 82.567 CPU sec, 2.605 real sec
sorting block 67, #reads = 1096749 mem = 536882571 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292481, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.07, 113.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702728 sequences (100000095 bp) in 2.209 CPU sec, 2.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293017, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.23, 115.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704528 reads in 130.914 CPU sec, 4.173 real sec
[M::mem_process_seqs] Processed 703462 reads in 82.504 CPU sec, 2.601 real sec
[M::process] read 702614 sequences (100000056 bp) in 1.583 CPU sec, 1.600 real sec...
sorting block 68, #reads = 1098205 mem = 536885444 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292797, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (240.99, 116.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1096385 mem = 536879461 peak = 1104531456
[M::process] read 702578 sequences (100000221 bp) in 2.378 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 292662, 6, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 172, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (192.64, 65.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 597)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.55, 115.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702728 reads in 135.921 CPU sec, 4.322 real sec
[M::process] read 701800 sequences (100000043 bp) in 1.482 CPU sec, 1.493 real sec...
[M::mem_process_seqs] Processed 702614 reads in 83.625 CPU sec, 2.641 real sec
sorting block 70, #reads = 1093515 mem = 536878422 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292097, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (241.20, 116.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 337, 1557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4303)
[M::mem_pestat] mean and std.dev: (693.83, 847.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5676)
[M::mem_pestat] skip orientation RR
[M::process] read 701756 sequences (100000049 bp) in 2.433 CPU sec, 2.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292392, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.97, 116.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702578 reads in 132.466 CPU sec, 4.214 real sec
[M::mem_process_seqs] Processed 701800 reads in 84.290 CPU sec, 2.655 real sec
[M::process] read 700094 sequences (100000109 bp) in 1.629 CPU sec, 1.644 real sec...
sorting block 71, #reads = 1095456 mem = 536882714 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291753, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.21, 115.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 380, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (329.00, 145.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RR
[M::process] read 699768 sequences (100000142 bp) in 2.298 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291518, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.47, 119.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701756 reads in 136.038 CPU sec, 4.331 real sec
[M::process] read 699604 sequences (100000086 bp) in 1.477 CPU sec, 1.483 real sec...
[M::mem_process_seqs] Processed 700094 reads in 86.525 CPU sec, 2.735 real sec
sorting block 72, #reads = 1094881 mem = 536878262 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291484, 11, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.40, 119.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (95, 191, 883)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2459)
[M::mem_pestat] mean and std.dev: (282.00, 292.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3247)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 73, #reads = 1091986 mem = 536881937 peak = 1104531456
[M::process] read 699134 sequences (100000137 bp) in 2.514 CPU sec, 2.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291930, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.09, 118.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 351, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (280.20, 81.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699768 reads in 138.912 CPU sec, 4.415 real sec
[M::process] read 699078 sequences (100000066 bp) in 1.549 CPU sec, 1.561 real sec...
[M::mem_process_seqs] Processed 699604 reads in 88.579 CPU sec, 2.796 real sec
sorting block 74, #reads = 1092376 mem = 536880673 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290937, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.79, 118.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698876 sequences (100000257 bp) in 2.327 CPU sec, 2.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291056, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (247.26, 118.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 236, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (227.40, 66.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RR
[M::process] read 699158 sequences (100000029 bp) in 1.480 CPU sec, 1.485 real sec...
[M::mem_process_seqs] Processed 699134 reads in 138.491 CPU sec, 4.397 real sec
[M::mem_process_seqs] Processed 699078 reads in 87.551 CPU sec, 2.762 real sec
sorting block 75, #reads = 1090809 mem = 536877918 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290842, 10, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.60, 118.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 495, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538)
[M::mem_pestat] mean and std.dev: (309.44, 271.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2027)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 711718 sequences (100000252 bp) in 2.509 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290882, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.31, 118.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 710060 sequences (100000229 bp) in 1.520 CPU sec, 1.517 real sec...
[M::mem_process_seqs] Processed 698876 reads in 149.677 CPU sec, 4.755 real sec
[M::mem_process_seqs] Processed 699158 reads in 98.508 CPU sec, 3.112 real sec
sorting block 76, #reads = 1090399 mem = 536878755 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 295104, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 196, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (218.07, 101.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 221, 789)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1979)
[M::mem_pestat] mean and std.dev: (325.70, 270.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2574)
[M::mem_pestat] skip orientation RR
[M::process] read 708190 sequences (100000054 bp) in 2.452 CPU sec, 2.545 real sec...
[M::process] read 706514 sequences (100000266 bp) in 1.490 CPU sec, 1.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 295088, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 200, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (222.82, 105.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 711718 reads in 148.454 CPU sec, 4.710 real sec
sorting block 77, #reads = 1083623 mem = 536882713 peak = 1104531456
[M::mem_process_seqs] Processed 710060 reads in 92.770 CPU sec, 2.941 real sec
sorting block 78, #reads = 1095442 mem = 536878373 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 294366, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 201, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159)
[M::mem_pestat] mean and std.dev: (259.82, 146.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1502)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (227.72, 108.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 705216 sequences (100000055 bp) in 2.491 CPU sec, 2.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293812, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 273, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (353.56, 193.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 208, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (233.06, 111.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 764)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 423, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (315.92, 114.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 704266 sequences (100000104 bp) in 1.500 CPU sec, 1.498 real sec...
[M::mem_process_seqs] Processed 708190 reads in 145.745 CPU sec, 4.624 real sec
[M::mem_process_seqs] Processed 706514 reads in 91.983 CPU sec, 2.911 real sec
sorting block 79, #reads = 1093963 mem = 536878468 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293545, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (235.83, 113.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 777)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 212, 1557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4337)
[M::mem_pestat] mean and std.dev: (685.22, 1032.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5727)
[M::mem_pestat] skip orientation RR
[M::process] read 704474 sequences (100000232 bp) in 2.482 CPU sec, 2.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293138, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 213, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (238.88, 115.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703504 sequences (100000279 bp) in 1.490 CPU sec, 1.483 real sec...
[M::mem_process_seqs] Processed 705216 reads in 142.318 CPU sec, 4.514 real sec
[M::mem_process_seqs] Processed 704266 reads in 89.467 CPU sec, 2.830 real sec
sorting block 80, #reads = 1091983 mem = 536880794 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292901, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (238.72, 116.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702624 sequences (100000060 bp) in 2.481 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292890, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (240.29, 116.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 235, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (250.69, 118.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RR
[M::process] read 700408 sequences (100000074 bp) in 1.470 CPU sec, 1.476 real sec...
[M::mem_process_seqs] Processed 704474 reads in 142.363 CPU sec, 4.524 real sec
sorting block 81, #reads = 1093062 mem = 536877562 peak = 1104531456
[M::mem_process_seqs] Processed 703504 reads in 88.441 CPU sec, 2.800 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292483, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (326, 395, 1385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3503)
[M::mem_pestat] mean and std.dev: (819.58, 842.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (238.73, 113.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 195, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (196.11, 67.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 575)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 82, #reads = 1093552 mem = 536878352 peak = 1104531456
[M::process] read 699228 sequences (100000165 bp) in 2.448 CPU sec, 2.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291727, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.58, 117.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699006 sequences (100000060 bp) in 1.460 CPU sec, 1.460 real sec...
[M::mem_process_seqs] Processed 702624 reads in 140.452 CPU sec, 4.456 real sec
[M::mem_process_seqs] Processed 700408 reads in 88.216 CPU sec, 2.792 real sec
sorting block 83, #reads = 1091403 mem = 536876790 peak = 1104531456
[M::process] read 698272 sequences (100000217 bp) in 2.460 CPU sec, 2.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291076, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 205, 978)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2606)
[M::mem_pestat] mean and std.dev: (403.08, 381.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3420)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (245.97, 116.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697686 sequences (100000171 bp) in 1.450 CPU sec, 1.453 real sec...
[M::mem_process_seqs] Processed 699006 reads in 79.254 CPU sec, 2.498 real sec
sorting block 84, #reads = 1090622 mem = 536878409 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291639, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.50, 117.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1749)
[M::mem_pestat] mean and std.dev: (369.25, 250.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2271)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290367, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (246.22, 115.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699228 reads in 211.166 CPU sec, 6.703 real sec
[M::process] read 697772 sequences (100000038 bp) in 1.779 CPU sec, 1.799 real sec...
[M::mem_process_seqs] Processed 698272 reads in 99.504 CPU sec, 3.159 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290680, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (248.39, 116.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 85, #reads = 1091489 mem = 536880728 peak = 1104531456
[M::process] read 697368 sequences (100000074 bp) in 2.554 CPU sec, 2.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290806, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 281562 sequences (40351500 bp) in 0.770 CPU sec, 0.770 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.84, 119.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697686 reads in 133.864 CPU sec, 4.272 real sec
[M::mem_process_seqs] Processed 697772 reads in 84.544 CPU sec, 2.683 real sec
sorting block 86, #reads = 1090673 mem = 536886365 peak = 1104531456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290043, 11, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 497, 716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742)
[M::mem_pestat] mean and std.dev: (444.70, 220.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2255)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.62, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 268, 2615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7345)
[M::mem_pestat] mean and std.dev: (593.44, 795.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9710)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 117080, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.12, 119.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697368 reads in 96.762 CPU sec, 3.145 real sec
[M::mem_process_seqs] Processed 281562 reads in 42.410 CPU sec, 1.373 real sec
sorting block 87, #reads = 1091731 mem = 536881236 peak = 1123995648
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96272516
overall: 70010769408 mem 11449.632 user 381.187 sys 507.641 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 507.684 sec; CPU: 11830.828 sec
sorting block 88, #reads = 530908 mem = 261827304 peak = 1123995648
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5226466193
output reads: 96763754
bam_mem_sort: 89 calls 35.048 user 0.595 sys 35.824 real
bam_write: 89 calls 33.995 user 1.435 sys 35.757 real
execute: 1 calls 17.557 user 0.995 sys 535.142 real
merge_files: 1 calls 17.547 user 0.964 sys 26.765 real
parse_chunk: 1416 calls 45.418 user 12.976 sys 59.399 real
read_chunk: 3825 calls 36.347 user 32.587 sys 337.866 real
sort_block: 1 calls 27.868 user 32.087 sys 325.787 real
write_chunk: 2407 calls 17.200 user 0.730 sys 25.931 real
overall: 3070984192 mem 1051.910 user 56.833 sys 535.167 real
ls: cannot access '*.cram': No such file or directory