cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_4Z5-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1875.677 CPU sec, 69.284 real sec [M::process] read 731128 sequences (100000165 bp) in 1.708 CPU sec, 1.710 real sec... [M::process] read 730318 sequences (100000201 bp) in 1.907 CPU sec, 1.913 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 299634, 52, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 442, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2539) [M::mem_pestat] mean and std.dev: (582.17, 469.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (201.18, 95.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 546, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3702) [M::mem_pestat] mean and std.dev: (726.62, 674.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4844) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 226, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1931) [M::mem_pestat] mean and std.dev: (418.30, 394.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2522) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 729226 sequences (100000130 bp) in 1.863 CPU sec, 1.872 real sec... [M::process] read 728132 sequences (100000269 bp) in 1.894 CPU sec, 1.923 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 299161, 47, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 529, 1693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4545) [M::mem_pestat] mean and std.dev: (937.10, 988.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (202.33, 96.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 458, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2830) [M::mem_pestat] mean and std.dev: (519.44, 487.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3736) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 331, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3457) [M::mem_pestat] mean and std.dev: (678.60, 724.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 731128 reads in 219.260 CPU sec, 6.942 real sec [M::mem_process_seqs] Processed 730318 reads in 150.921 CPU sec, 4.837 real sec sorting block 0, #reads = 1097894 mem = 536879070 peak = 605134848 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 299273, 41, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 333, 1280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3414) [M::mem_pestat] mean and std.dev: (784.07, 861.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.49, 97.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (470, 705, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2936) [M::mem_pestat] mean and std.dev: (734.08, 475.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 250, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242) [M::mem_pestat] mean and std.dev: (304.14, 219.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 727570 sequences (100000208 bp) in 2.827 CPU sec, 3.172 real sec... [M::process] read 727252 sequences (100000181 bp) in 2.070 CPU sec, 2.079 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 298569, 51, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 675, 1347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3467) [M::mem_pestat] mean and std.dev: (917.24, 841.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 185, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (206.44, 99.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (637, 997, 1317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2677) [M::mem_pestat] mean and std.dev: (884.87, 573.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 491, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2366) [M::mem_pestat] mean and std.dev: (640.20, 568.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 729226 reads in 212.187 CPU sec, 6.768 real sec [M::mem_process_seqs] Processed 728132 reads in 148.751 CPU sec, 4.765 real sec sorting block 1, #reads = 1097932 mem = 536881620 peak = 763994112 [M::process] read 727486 sequences (100000095 bp) in 3.006 CPU sec, 3.256 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 298588, 37, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 335, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (420.10, 379.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 186, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (207.68, 99.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 977, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3647) [M::mem_pestat] mean and std.dev: (845.19, 663.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 287, 1392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3866) [M::mem_pestat] mean and std.dev: (795.83, 910.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 726994 sequences (100000191 bp) in 2.213 CPU sec, 2.216 real sec... [M::mem_process_seqs] Processed 727252 reads in 120.169 CPU sec, 3.827 real sec sorting block 2, #reads = 1095527 mem = 536884472 peak = 810590208 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 298921, 41, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 466, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1894) [M::mem_pestat] mean and std.dev: (520.41, 441.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 186, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.17, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 490, 1430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4150) [M::mem_pestat] mean and std.dev: (733.77, 808.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 485, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909) [M::mem_pestat] mean and std.dev: (641.70, 686.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 298264, 57, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 347, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2806) [M::mem_pestat] mean and std.dev: (570.02, 536.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 186, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (207.59, 100.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 501, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012) [M::mem_pestat] mean and std.dev: (549.54, 390.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 543, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3706) [M::mem_pestat] mean and std.dev: (733.37, 647.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727570 reads in 328.444 CPU sec, 10.520 real sec [M::process] read 726382 sequences (100000123 bp) in 2.683 CPU sec, 2.725 real sec... [M::mem_process_seqs] Processed 727486 reads in 180.042 CPU sec, 5.831 real sec sorting block 3, #reads = 1095893 mem = 536879205 peak = 810590208 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 298220, 49, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 305, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1805) [M::mem_pestat] mean and std.dev: (441.91, 380.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 187, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (209.01, 101.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (477, 788, 1936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4854) [M::mem_pestat] mean and std.dev: (1180.21, 1018.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6313) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 455, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2443) [M::mem_pestat] mean and std.dev: (536.62, 377.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3165) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 725708 sequences (100000157 bp) in 3.742 CPU sec, 4.171 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 297762, 29, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 363, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1945) [M::mem_pestat] mean and std.dev: (442.03, 386.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (209.36, 101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (334, 564, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1717) [M::mem_pestat] mean and std.dev: (533.43, 296.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 336, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1676) [M::mem_pestat] mean and std.dev: (374.10, 261.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726994 reads in 212.883 CPU sec, 6.829 real sec [M::mem_process_seqs] Processed 726382 reads in 152.211 CPU sec, 4.824 real sec [M::process] read 724948 sequences (100000120 bp) in 2.873 CPU sec, 2.990 real sec... sorting block 4, #reads = 1096305 mem = 536880418 peak = 810590208 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 297883, 40, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 303, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1836) [M::mem_pestat] mean and std.dev: (413.24, 387.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 188, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (210.00, 101.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (348, 630, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2838) [M::mem_pestat] mean and std.dev: (658.58, 465.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 348, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2032) [M::mem_pestat] mean and std.dev: (530.62, 526.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724810 sequences (100000285 bp) in 3.047 CPU sec, 3.597 real sec... sorting block 5, #reads = 1095956 mem = 536879261 peak = 851939328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 297954, 45, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 356, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4398) [M::mem_pestat] mean and std.dev: (946.29, 1051.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (210.93, 102.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 773, 1875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5337) [M::mem_pestat] mean and std.dev: (879.00, 981.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 330, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2420) [M::mem_pestat] mean and std.dev: (522.60, 528.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725708 reads in 216.105 CPU sec, 7.025 real sec [M::process] read 724588 sequences (100000010 bp) in 2.802 CPU sec, 2.918 real sec... [M::mem_process_seqs] Processed 724948 reads in 155.643 CPU sec, 4.990 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 297857, 52, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 601, 1444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3974) [M::mem_pestat] mean and std.dev: (805.45, 761.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.00, 101.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (105, 446, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2766) [M::mem_pestat] mean and std.dev: (492.47, 488.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 506, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2623) [M::mem_pestat] mean and std.dev: (562.50, 405.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 6, #reads = 1096390 mem = 536882012 peak = 851939328 [M::process] read 724416 sequences (100000002 bp) in 3.525 CPU sec, 4.133 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 297461, 39, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 602, 1774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4836) [M::mem_pestat] mean and std.dev: (742.56, 626.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (210.86, 101.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (97, 218, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2107) [M::mem_pestat] mean and std.dev: (380.86, 463.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 314, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340) [M::mem_pestat] mean and std.dev: (386.49, 284.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724810 reads in 216.150 CPU sec, 7.040 real sec [M::mem_process_seqs] Processed 724588 reads in 153.636 CPU sec, 4.953 real sec [M::process] read 723646 sequences (100000231 bp) in 2.797 CPU sec, 3.092 real sec... sorting block 7, #reads = 1094251 mem = 536880234 peak = 851939328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 297516, 39, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 273, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2683) [M::mem_pestat] mean and std.dev: (563.52, 509.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (211.80, 102.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 573, 1884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5184) [M::mem_pestat] mean and std.dev: (1026.22, 1204.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 263, 879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2271) [M::mem_pestat] mean and std.dev: (398.07, 398.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723436 sequences (100000000 bp) in 3.485 CPU sec, 4.072 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 297128, 34, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 322, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (507.41, 560.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.62, 101.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 379, 1162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3356) [M::mem_pestat] mean and std.dev: (559.83, 788.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (334, 615, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2266) [M::mem_pestat] mean and std.dev: (625.08, 443.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724416 reads in 218.396 CPU sec, 7.128 real sec [M::mem_process_seqs] Processed 723646 reads in 154.711 CPU sec, 5.028 real sec sorting block 8, #reads = 1095476 mem = 536879886 peak = 851939328 [M::process] read 723540 sequences (100000139 bp) in 3.082 CPU sec, 3.378 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 296623, 44, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 399, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2116) [M::mem_pestat] mean and std.dev: (478.28, 335.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.72, 101.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (408, 727, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3162) [M::mem_pestat] mean and std.dev: (693.92, 482.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 377, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1901) [M::mem_pestat] mean and std.dev: (466.04, 363.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723766 sequences (100000169 bp) in 3.626 CPU sec, 4.040 real sec... sorting block 9, #reads = 1093404 mem = 536879155 peak = 851939328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 296964, 45, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 234, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1915) [M::mem_pestat] mean and std.dev: (486.67, 508.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (210.97, 101.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 809, 1480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4000) [M::mem_pestat] mean and std.dev: (835.84, 638.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 344, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032) [M::mem_pestat] mean and std.dev: (315.52, 125.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723436 reads in 223.226 CPU sec, 7.350 real sec [M::mem_process_seqs] Processed 723540 reads in 156.091 CPU sec, 5.152 real sec [M::process] read 724248 sequences (100000273 bp) in 3.465 CPU sec, 3.880 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 297255, 38, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 410, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2791) [M::mem_pestat] mean and std.dev: (654.04, 678.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.17, 101.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (170, 459, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2702) [M::mem_pestat] mean and std.dev: (607.47, 609.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3546) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 325, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437) [M::mem_pestat] mean and std.dev: (349.37, 196.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 10, #reads = 1092456 mem = 536877303 peak = 851939328 [M::process] read 723558 sequences (100000156 bp) in 3.486 CPU sec, 3.973 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 297232, 41, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 413, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3267) [M::mem_pestat] mean and std.dev: (456.60, 411.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.09, 100.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (203, 672, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2846) [M::mem_pestat] mean and std.dev: (676.33, 552.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 337, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831) [M::mem_pestat] mean and std.dev: (442.00, 405.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723766 reads in 224.451 CPU sec, 7.444 real sec [M::mem_process_seqs] Processed 724248 reads in 157.436 CPU sec, 5.228 real sec [M::process] read 724038 sequences (100000154 bp) in 4.068 CPU sec, 4.483 real sec... sorting block 11, #reads = 1092992 mem = 536877960 peak = 851939328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 296668, 36, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 402, 1784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4946) [M::mem_pestat] mean and std.dev: (952.10, 1198.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (210.20, 100.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 553, 1792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5080) [M::mem_pestat] mean and std.dev: (1153.83, 1367.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6724) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 522, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4056) [M::mem_pestat] mean and std.dev: (723.05, 616.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 296755, 34, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 235, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3488) [M::mem_pestat] mean and std.dev: (554.98, 662.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.08, 101.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (147, 622, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3675) [M::mem_pestat] mean and std.dev: (691.37, 637.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 348, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (347.02, 172.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723474 sequences (100000000 bp) in 3.322 CPU sec, 3.603 real sec... [M::mem_process_seqs] Processed 723558 reads in 226.320 CPU sec, 7.487 real sec [M::mem_process_seqs] Processed 724038 reads in 158.383 CPU sec, 5.219 real sec sorting block 12, #reads = 1093391 mem = 536877774 peak = 851939328 [M::process] read 723016 sequences (100000238 bp) in 3.396 CPU sec, 3.735 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 297065, 42, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 428, 1713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4721) [M::mem_pestat] mean and std.dev: (733.64, 828.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.27, 101.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 549, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4742) [M::mem_pestat] mean and std.dev: (922.71, 875.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 516, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2032) [M::mem_pestat] mean and std.dev: (523.73, 332.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723474 reads in 126.860 CPU sec, 4.273 real sec [M::process] read 723002 sequences (100000283 bp) in 3.759 CPU sec, 4.220 real sec... sorting block 13, #reads = 1093588 mem = 536879987 peak = 851939328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 296962, 41, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 586, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2390) [M::mem_pestat] mean and std.dev: (612.29, 450.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.28, 100.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 576, 1117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3045) [M::mem_pestat] mean and std.dev: (604.21, 577.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 521, 1364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3692) [M::mem_pestat] mean and std.dev: (846.77, 812.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723016 reads in 155.699 CPU sec, 5.373 real sec [M::process] read 722570 sequences (100000197 bp) in 3.631 CPU sec, 4.031 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 297336, 29, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 325, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2058) [M::mem_pestat] mean and std.dev: (497.15, 467.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.77, 101.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 402, 1343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3475) [M::mem_pestat] mean and std.dev: (695.48, 751.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (332, 491, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2240) [M::mem_pestat] mean and std.dev: (645.80, 477.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 14, #reads = 1092854 mem = 536877221 peak = 851939328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 296498, 42, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 312, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3397) [M::mem_pestat] mean and std.dev: (744.59, 881.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.47, 101.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 622, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3394) [M::mem_pestat] mean and std.dev: (752.62, 687.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 387, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1468) [M::mem_pestat] mean and std.dev: (453.33, 304.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 721880 sequences (100000105 bp) in 3.552 CPU sec, 3.941 real sec... [M::mem_process_seqs] Processed 723002 reads in 263.419 CPU sec, 8.897 real sec [M::mem_process_seqs] Processed 722570 reads in 157.040 CPU sec, 5.287 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 296446, 54, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 304, 1399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3881) [M::mem_pestat] mean and std.dev: (470.19, 591.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 192, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.36, 101.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 333, 1455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4327) [M::mem_pestat] mean and std.dev: (740.18, 1010.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 323, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1540) [M::mem_pestat] mean and std.dev: (417.31, 332.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 720660 sequences (100000203 bp) in 3.711 CPU sec, 4.072 real sec... sorting block 15, #reads = 1093224 mem = 536878204 peak = 851939328 [M::mem_process_seqs] Processed 721880 reads in 130.057 CPU sec, 4.373 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 296096, 31, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 417, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3323) [M::mem_pestat] mean and std.dev: (706.11, 695.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (216.48, 104.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (409, 662, 2142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5608) [M::mem_pestat] mean and std.dev: (1038.58, 855.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 329, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153) [M::mem_pestat] mean and std.dev: (376.53, 317.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 721696 sequences (100000097 bp) in 3.700 CPU sec, 4.234 real sec... [M::mem_process_seqs] Processed 720660 reads in 121.085 CPU sec, 4.156 real sec sorting block 16, #reads = 1093415 mem = 536878139 peak = 851939328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 296402, 55, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 391, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2236) [M::mem_pestat] mean and std.dev: (568.97, 390.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 192, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (213.68, 101.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (141, 428, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3030) [M::mem_pestat] mean and std.dev: (613.60, 641.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 340, 1530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4258) [M::mem_pestat] mean and std.dev: (664.86, 794.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 721542 sequences (100000110 bp) in 3.534 CPU sec, 3.915 real sec... [M::mem_process_seqs] Processed 721696 reads in 126.246 CPU sec, 4.522 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 296627, 34, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 299, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2186) [M::mem_pestat] mean and std.dev: (515.57, 583.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 193, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (214.50, 102.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 438, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432) [M::mem_pestat] mean and std.dev: (405.83, 216.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 348, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2631) [M::mem_pestat] mean and std.dev: (622.68, 624.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3446) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 720710 sequences (100000201 bp) in 3.673 CPU sec, 4.028 real sec... sorting block 17, #reads = 1093147 mem = 536884857 peak = 851939328 [M::mem_process_seqs] Processed 721542 reads in 127.146 CPU sec, 4.492 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 296251, 48, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 291, 991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2629) [M::mem_pestat] mean and std.dev: (537.95, 570.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (215.07, 102.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (175, 814, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3931) [M::mem_pestat] mean and std.dev: (727.00, 631.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 342, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2736) [M::mem_pestat] mean and std.dev: (541.06, 477.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 720496 sequences (100000191 bp) in 3.414 CPU sec, 3.664 real sec... [M::mem_process_seqs] Processed 720710 reads in 124.937 CPU sec, 4.237 real sec sorting block 18, #reads = 1093422 mem = 536882175 peak = 851939328 [M::process] read 720458 sequences (100000248 bp) in 2.996 CPU sec, 3.305 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 295681, 47, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 385, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (349.72, 201.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (215.22, 102.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 462, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2671) [M::mem_pestat] mean and std.dev: (684.63, 642.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 450, 1703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4693) [M::mem_pestat] mean and std.dev: (883.33, 836.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 19, #reads = 1092564 mem = 536882380 peak = 854872064 [M::process] read 720424 sequences (100000215 bp) in 3.560 CPU sec, 3.808 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 295979, 43, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 321, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651) [M::mem_pestat] mean and std.dev: (439.37, 351.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2134) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 195, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.91, 103.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (361, 478, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741) [M::mem_pestat] mean and std.dev: (573.02, 393.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 373, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2035) [M::mem_pestat] mean and std.dev: (511.26, 458.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 720496 reads in 223.403 CPU sec, 7.382 real sec [M::mem_process_seqs] Processed 720458 reads in 158.575 CPU sec, 5.259 real sec [M::process] read 720272 sequences (100000072 bp) in 2.643 CPU sec, 3.022 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 296033, 36, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 511, 1431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3701) [M::mem_pestat] mean and std.dev: (863.46, 926.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 195, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.86, 103.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (583, 843, 2129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5221) [M::mem_pestat] mean and std.dev: (1190.88, 937.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 578, 1939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5395) [M::mem_pestat] mean and std.dev: (903.22, 867.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 20, #reads = 1092019 mem = 536879507 peak = 854872064 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 296194, 56, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 321, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475) [M::mem_pestat] mean and std.dev: (366.87, 302.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 719962 sequences (100000266 bp) in 3.816 CPU sec, 4.277 real sec... [M::mem_pestat] (25, 50, 75) percentile: (136, 195, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (216.02, 103.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 660, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587) [M::mem_pestat] mean and std.dev: (806.69, 755.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 786, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3455) [M::mem_pestat] mean and std.dev: (875.98, 777.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 720424 reads in 223.025 CPU sec, 7.322 real sec [M::mem_process_seqs] Processed 720272 reads in 156.917 CPU sec, 5.105 real sec [M::process] read 722916 sequences (100000005 bp) in 3.335 CPU sec, 3.687 real sec... sorting block 21, #reads = 1092230 mem = 536876954 peak = 854872064 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 296131, 71, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 346, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2178) [M::mem_pestat] mean and std.dev: (590.61, 520.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 196, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (217.61, 104.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 1312, 3085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8367) [M::mem_pestat] mean and std.dev: (1883.72, 1842.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 518, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3520) [M::mem_pestat] mean and std.dev: (661.32, 630.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4626) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 297059, 37, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 491, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3505) [M::mem_pestat] mean and std.dev: (776.53, 715.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (212.67, 101.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (350, 972, 2035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5405) [M::mem_pestat] mean and std.dev: (1228.22, 1154.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7090) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 671, 1320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3452) [M::mem_pestat] mean and std.dev: (722.51, 688.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4518) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 730610 sequences (100000089 bp) in 3.447 CPU sec, 3.978 real sec... [M::mem_process_seqs] Processed 719962 reads in 219.302 CPU sec, 7.197 real sec sorting block 22, #reads = 1092975 mem = 536878268 peak = 854872064 [M::process] read 729810 sequences (100000168 bp) in 3.203 CPU sec, 3.810 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 299302, 35, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 405, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2699) [M::mem_pestat] mean and std.dev: (574.67, 551.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (202.08, 96.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 493, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2716) [M::mem_pestat] mean and std.dev: (507.06, 447.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 414, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1734) [M::mem_pestat] mean and std.dev: (447.93, 330.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 730610 reads in 126.119 CPU sec, 4.075 real sec [M::process] read 729196 sequences (100000216 bp) in 3.461 CPU sec, 3.676 real sec... sorting block 23, #reads = 1095111 mem = 536882176 peak = 866553856 [M::mem_process_seqs] Processed 722916 reads in 374.669 CPU sec, 12.084 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 298961, 33, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 345, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3428) [M::mem_pestat] mean and std.dev: (675.38, 667.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 183, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (203.86, 97.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 468, 2016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5490) [M::mem_pestat] mean and std.dev: (1152.09, 1327.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 468, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3371) [M::mem_pestat] mean and std.dev: (737.15, 734.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 298655, 50, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 433, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2157) [M::mem_pestat] mean and std.dev: (529.32, 450.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.06, 98.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 441, 1581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4453) [M::mem_pestat] mean and std.dev: (701.04, 696.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 341, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2807) [M::mem_pestat] mean and std.dev: (664.33, 614.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 728528 sequences (100000240 bp) in 3.384 CPU sec, 4.470 real sec... [M::mem_process_seqs] Processed 729810 reads in 251.593 CPU sec, 8.119 real sec [M::mem_process_seqs] Processed 729196 reads in 154.126 CPU sec, 5.024 real sec sorting block 24, #reads = 1093454 mem = 536877291 peak = 866553856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 299271, 26, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 406, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3447) [M::mem_pestat] mean and std.dev: (584.06, 548.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 185, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (205.85, 99.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 468, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2292) [M::mem_pestat] mean and std.dev: (414.30, 372.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3027) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 333, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061) [M::mem_pestat] mean and std.dev: (467.02, 396.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 728194 sequences (100000052 bp) in 3.601 CPU sec, 3.963 real sec... [M::mem_process_seqs] Processed 728528 reads in 123.992 CPU sec, 4.152 real sec [M::process] read 727360 sequences (100000259 bp) in 3.530 CPU sec, 3.992 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 298929, 28, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 460, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2476) [M::mem_pestat] mean and std.dev: (562.17, 487.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 185, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (206.48, 99.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (476, 1079, 1283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2897) [M::mem_pestat] mean and std.dev: (855.96, 590.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 461, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2748) [M::mem_pestat] mean and std.dev: (690.11, 726.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 25, #reads = 1097809 mem = 536884271 peak = 866553856 [M::mem_process_seqs] Processed 728194 reads in 121.496 CPU sec, 4.143 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 298613, 40, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 434, 1501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4087) [M::mem_pestat] mean and std.dev: (715.31, 851.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 186, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (207.30, 99.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (249, 757, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3276) [M::mem_pestat] mean and std.dev: (690.94, 605.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 379, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2554) [M::mem_pestat] mean and std.dev: (583.63, 431.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 726726 sequences (100000240 bp) in 3.673 CPU sec, 4.132 real sec... [M::mem_process_seqs] Processed 727360 reads in 123.200 CPU sec, 4.171 real sec sorting block 26, #reads = 1097597 mem = 536883756 peak = 866553856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 298614, 39, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 289, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175) [M::mem_pestat] mean and std.dev: (459.21, 489.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 187, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (208.59, 100.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 473, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2784) [M::mem_pestat] mean and std.dev: (687.42, 661.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 364, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2450) [M::mem_pestat] mean and std.dev: (563.99, 551.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 726140 sequences (100000257 bp) in 3.508 CPU sec, 3.837 real sec... [M::mem_process_seqs] Processed 726726 reads in 124.009 CPU sec, 4.277 real sec sorting block 27, #reads = 1096352 mem = 536878483 peak = 887877632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 298386, 45, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 527, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2669) [M::mem_pestat] mean and std.dev: (643.74, 573.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 188, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (210.30, 102.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (428, 781, 1440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3464) [M::mem_pestat] mean and std.dev: (663.08, 542.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 346, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2782) [M::mem_pestat] mean and std.dev: (626.97, 588.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3633) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 726284 sequences (100000125 bp) in 3.366 CPU sec, 3.725 real sec... [M::mem_process_seqs] Processed 726140 reads in 124.915 CPU sec, 4.176 real sec [M::process] read 726168 sequences (100000206 bp) in 3.195 CPU sec, 3.744 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 298342, 33, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 540, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2242) [M::mem_pestat] mean and std.dev: (545.23, 466.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (209.17, 101.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 560, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4031) [M::mem_pestat] mean and std.dev: (837.67, 902.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 526, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2854) [M::mem_pestat] mean and std.dev: (634.92, 525.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726284 reads in 124.330 CPU sec, 4.191 real sec sorting block 28, #reads = 1096992 mem = 536882238 peak = 887877632 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 297941, 50, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 346, 1543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4299) [M::mem_pestat] mean and std.dev: (779.78, 952.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 188, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (210.08, 101.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (518, 848, 2435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6269) [M::mem_pestat] mean and std.dev: (1420.10, 1274.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8186) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 287, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1511) [M::mem_pestat] mean and std.dev: (365.07, 243.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 725218 sequences (100000097 bp) in 3.625 CPU sec, 4.165 real sec... [M::mem_process_seqs] Processed 726168 reads in 122.248 CPU sec, 4.171 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 298002, 38, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 283, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852) [M::mem_pestat] mean and std.dev: (367.19, 314.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 188, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (210.83, 102.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 660, 2049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5757) [M::mem_pestat] mean and std.dev: (1222.38, 1362.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 560, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4934) [M::mem_pestat] mean and std.dev: (1057.60, 1235.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6508) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 29, #reads = 1096576 mem = 536890039 peak = 888950784 [M::process] read 725004 sequences (100000194 bp) in 4.218 CPU sec, 4.656 real sec... [M::mem_process_seqs] Processed 725218 reads in 122.469 CPU sec, 4.132 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 297566, 60, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 325, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (383.54, 237.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.23, 102.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (228, 492, 1385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3699) [M::mem_pestat] mean and std.dev: (720.79, 640.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 492, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4815) [M::mem_pestat] mean and std.dev: (996.05, 1157.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724364 sequences (100000035 bp) in 3.780 CPU sec, 4.151 real sec... [M::mem_process_seqs] Processed 725004 reads in 122.291 CPU sec, 4.137 real sec sorting block 30, #reads = 1096048 mem = 536878223 peak = 888950784 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 297524, 53, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 376, 872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172) [M::mem_pestat] mean and std.dev: (576.26, 519.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (212.15, 102.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 380, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437) [M::mem_pestat] mean and std.dev: (369.32, 284.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 540, 1438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3894) [M::mem_pestat] mean and std.dev: (848.54, 910.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724144 sequences (100000259 bp) in 3.365 CPU sec, 3.704 real sec... [M::mem_process_seqs] Processed 724364 reads in 123.083 CPU sec, 4.194 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 297055, 57, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 289, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2048) [M::mem_pestat] mean and std.dev: (415.04, 389.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.80, 102.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (147, 531, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2250) [M::mem_pestat] mean and std.dev: (461.90, 447.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 410, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4079) [M::mem_pestat] mean and std.dev: (736.50, 705.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 31, #reads = 1095693 mem = 536878009 peak = 898555904 [M::process] read 724104 sequences (100000110 bp) in 3.811 CPU sec, 4.495 real sec... [M::mem_process_seqs] Processed 724144 reads in 124.883 CPU sec, 4.195 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 297530, 43, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 314, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792) [M::mem_pestat] mean and std.dev: (401.08, 303.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.59, 102.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (369, 579, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172) [M::mem_pestat] mean and std.dev: (657.41, 466.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 326, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2482) [M::mem_pestat] mean and std.dev: (517.87, 537.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724332 sequences (100000229 bp) in 3.287 CPU sec, 3.655 real sec... [M::mem_process_seqs] Processed 724104 reads in 122.633 CPU sec, 4.151 real sec sorting block 32, #reads = 1095384 mem = 536879218 peak = 898555904 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 297980, 39, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 235, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (322.27, 301.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.62, 102.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (131, 581, 1718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4892) [M::mem_pestat] mean and std.dev: (876.05, 880.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 356, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792) [M::mem_pestat] mean and std.dev: (411.07, 319.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723994 sequences (100000281 bp) in 3.971 CPU sec, 4.547 real sec... [M::mem_process_seqs] Processed 724332 reads in 122.220 CPU sec, 4.130 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 296807, 67, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 309, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2945) [M::mem_pestat] mean and std.dev: (530.18, 594.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.00, 101.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 563, 1421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3927) [M::mem_pestat] mean and std.dev: (865.48, 947.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5180) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 332, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301) [M::mem_pestat] mean and std.dev: (766.53, 871.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 33, #reads = 1093623 mem = 536880350 peak = 898555904 [M::process] read 724656 sequences (100000125 bp) in 3.515 CPU sec, 3.811 real sec... [M::mem_process_seqs] Processed 723994 reads in 122.005 CPU sec, 4.150 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 297262, 49, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 381, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2490) [M::mem_pestat] mean and std.dev: (490.52, 417.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (209.73, 100.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 369, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2403) [M::mem_pestat] mean and std.dev: (453.98, 427.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 468, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1887) [M::mem_pestat] mean and std.dev: (465.15, 340.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724666 sequences (100000213 bp) in 3.203 CPU sec, 3.771 real sec... [M::mem_process_seqs] Processed 724656 reads in 123.637 CPU sec, 4.208 real sec sorting block 34, #reads = 1094175 mem = 536877309 peak = 898625536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 297729, 35, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 402, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3612) [M::mem_pestat] mean and std.dev: (698.67, 609.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.03, 101.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 767, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4892) [M::mem_pestat] mean and std.dev: (1009.21, 906.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 430, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2906) [M::mem_pestat] mean and std.dev: (701.80, 735.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723926 sequences (100000039 bp) in 3.478 CPU sec, 3.845 real sec... [M::mem_process_seqs] Processed 724666 reads in 124.265 CPU sec, 4.224 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 296486, 42, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 479, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422) [M::mem_pestat] mean and std.dev: (636.79, 488.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.55, 101.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 731, 1677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4275) [M::mem_pestat] mean and std.dev: (727.69, 558.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 465, 1576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4196) [M::mem_pestat] mean and std.dev: (808.26, 909.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724042 sequences (100000039 bp) in 3.583 CPU sec, 3.886 real sec... sorting block 35, #reads = 1093225 mem = 536878716 peak = 898625536 [M::mem_process_seqs] Processed 723926 reads in 124.555 CPU sec, 4.248 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 297309, 51, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 394, 1352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3560) [M::mem_pestat] mean and std.dev: (718.16, 638.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 724176 sequences (100000153 bp) in 3.348 CPU sec, 3.699 real sec... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.34, 100.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (384, 585, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2358) [M::mem_pestat] mean and std.dev: (717.06, 565.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3016) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (296, 481, 1137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (670.89, 492.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3660) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724042 reads in 124.104 CPU sec, 4.309 real sec sorting block 36, #reads = 1093839 mem = 536883200 peak = 898625536 [M::process] read 723578 sequences (100000056 bp) in 3.377 CPU sec, 3.765 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 297452, 44, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 626, 1208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3198) [M::mem_pestat] mean and std.dev: (750.52, 612.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.26, 100.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 1015, 2708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7766) [M::mem_pestat] mean and std.dev: (1462.23, 1344.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 365, 911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2271) [M::mem_pestat] mean and std.dev: (497.45, 420.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 296785, 46, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 455, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3600) [M::mem_pestat] mean and std.dev: (679.84, 602.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (210.94, 100.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 579, 1460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4162) [M::mem_pestat] mean and std.dev: (976.77, 1070.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5513) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 306, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1765) [M::mem_pestat] mean and std.dev: (393.58, 274.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723178 sequences (100000285 bp) in 3.405 CPU sec, 3.779 real sec... sorting block 37, #reads = 1094262 mem = 536879012 peak = 937160704 [M::mem_process_seqs] Processed 724176 reads in 218.905 CPU sec, 7.191 real sec [M::mem_process_seqs] Processed 723578 reads in 154.973 CPU sec, 5.079 real sec [M::process] read 722746 sequences (100000299 bp) in 3.373 CPU sec, 3.790 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 296495, 31, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 444, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2564) [M::mem_pestat] mean and std.dev: (594.24, 533.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.79, 101.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (469, 853, 1137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2473) [M::mem_pestat] mean and std.dev: (719.52, 455.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3141) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 431, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027) [M::mem_pestat] mean and std.dev: (503.29, 409.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723178 reads in 124.902 CPU sec, 4.228 real sec sorting block 38, #reads = 1093337 mem = 536879913 peak = 937160704 [M::process] read 722602 sequences (100000145 bp) in 3.419 CPU sec, 3.607 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 296523, 45, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 230, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1674) [M::mem_pestat] mean and std.dev: (379.70, 395.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.26, 100.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 500, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2675) [M::mem_pestat] mean and std.dev: (658.81, 592.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 377, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2117) [M::mem_pestat] mean and std.dev: (531.54, 405.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 296818, 41, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 351, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2552) [M::mem_pestat] mean and std.dev: (594.76, 615.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.51, 101.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 455, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2866) [M::mem_pestat] mean and std.dev: (671.30, 599.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 549, 1470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3888) [M::mem_pestat] mean and std.dev: (729.63, 673.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723398 sequences (100000151 bp) in 3.181 CPU sec, 3.456 real sec... sorting block 39, #reads = 1093049 mem = 536890525 peak = 937160704 [M::mem_process_seqs] Processed 722746 reads in 248.023 CPU sec, 8.313 real sec [M::mem_process_seqs] Processed 722602 reads in 154.417 CPU sec, 5.268 real sec [M::process] read 722410 sequences (100000213 bp) in 2.714 CPU sec, 2.930 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 296665, 41, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 354, 1790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5026) [M::mem_pestat] mean and std.dev: (884.41, 1142.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.04, 102.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 495, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4097) [M::mem_pestat] mean and std.dev: (840.19, 975.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5420) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 328, 1569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4389) [M::mem_pestat] mean and std.dev: (803.55, 871.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 40, #reads = 1092276 mem = 536881249 peak = 937160704 [M::process] read 722616 sequences (100000204 bp) in 3.031 CPU sec, 3.369 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 296456, 48, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 361, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2851) [M::mem_pestat] mean and std.dev: (665.17, 653.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.79, 102.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 831, 1498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4138) [M::mem_pestat] mean and std.dev: (791.33, 811.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 636, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2405) [M::mem_pestat] mean and std.dev: (609.82, 497.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723398 reads in 218.541 CPU sec, 7.075 real sec [M::mem_process_seqs] Processed 722410 reads in 155.700 CPU sec, 4.990 real sec [M::process] read 722068 sequences (100000001 bp) in 2.609 CPU sec, 2.662 real sec... sorting block 41, #reads = 1093059 mem = 536881060 peak = 937160704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 296846, 53, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 329, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1617) [M::mem_pestat] mean and std.dev: (472.46, 399.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (213.50, 103.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (236, 476, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1856) [M::mem_pestat] mean and std.dev: (497.69, 391.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 472, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3590) [M::mem_pestat] mean and std.dev: (765.48, 682.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 721460 sequences (100000035 bp) in 3.182 CPU sec, 3.470 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 296244, 41, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 766, 1757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4783) [M::mem_pestat] mean and std.dev: (995.46, 1032.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 192, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.15, 102.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 668, 1066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2486) [M::mem_pestat] mean and std.dev: (640.14, 412.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 410, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3041) [M::mem_pestat] mean and std.dev: (657.26, 610.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 722616 reads in 215.462 CPU sec, 6.984 real sec [M::process] read 721102 sequences (100000202 bp) in 2.946 CPU sec, 2.974 real sec... sorting block 42, #reads = 1092421 mem = 536882919 peak = 937160704 [M::mem_process_seqs] Processed 722068 reads in 157.592 CPU sec, 5.060 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 296374, 43, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 303, 1561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4235) [M::mem_pestat] mean and std.dev: (790.47, 955.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 192, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (213.63, 101.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (101, 496, 1061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2981) [M::mem_pestat] mean and std.dev: (432.63, 394.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (325, 419, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3001) [M::mem_pestat] mean and std.dev: (708.16, 574.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 43, #reads = 1092481 mem = 536883695 peak = 998248448 [M::process] read 721160 sequences (100000196 bp) in 3.812 CPU sec, 4.194 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 296234, 64, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 446, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1903) [M::mem_pestat] mean and std.dev: (443.67, 325.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 193, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (214.67, 102.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 565, 1554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4182) [M::mem_pestat] mean and std.dev: (917.29, 964.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 366, 1889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5307) [M::mem_pestat] mean and std.dev: (788.17, 931.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 721460 reads in 214.196 CPU sec, 6.962 real sec [M::mem_process_seqs] Processed 721102 reads in 152.674 CPU sec, 4.932 real sec [M::process] read 720880 sequences (100000103 bp) in 3.209 CPU sec, 3.436 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 296344, 49, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 297, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3221) [M::mem_pestat] mean and std.dev: (549.63, 508.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.74, 103.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (316, 577, 2856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7936) [M::mem_pestat] mean and std.dev: (1428.53, 1610.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 494, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1851) [M::mem_pestat] mean and std.dev: (532.61, 381.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2379) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 44, #reads = 1093368 mem = 536879802 peak = 998248448 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 296613, 33, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 262, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327) [M::mem_pestat] mean and std.dev: (339.18, 228.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.77, 104.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (333, 835, 2544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6966) [M::mem_pestat] mean and std.dev: (1149.57, 1144.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 310, 1718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4740) [M::mem_pestat] mean and std.dev: (920.80, 967.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 720852 sequences (100000168 bp) in 4.063 CPU sec, 4.616 real sec... [M::mem_process_seqs] Processed 721160 reads in 215.995 CPU sec, 7.028 real sec [M::mem_process_seqs] Processed 720880 reads in 154.548 CPU sec, 4.990 real sec sorting block 45, #reads = 1091510 mem = 536877199 peak = 998248448 [M::process] read 729784 sequences (100000083 bp) in 3.753 CPU sec, 4.105 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 296459, 46, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 719, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2905) [M::mem_pestat] mean and std.dev: (843.40, 718.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (216.88, 105.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (253, 554, 1362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3580) [M::mem_pestat] mean and std.dev: (874.47, 804.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 376, 1249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329) [M::mem_pestat] mean and std.dev: (697.43, 675.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 298732, 31, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 255, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1829) [M::mem_pestat] mean and std.dev: (413.20, 387.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 183, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (203.74, 97.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (123, 433, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2760) [M::mem_pestat] mean and std.dev: (569.73, 576.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 361, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1918) [M::mem_pestat] mean and std.dev: (545.34, 405.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 730412 sequences (100000260 bp) in 3.508 CPU sec, 4.054 real sec... [M::mem_process_seqs] Processed 720852 reads in 222.822 CPU sec, 7.290 real sec [M::mem_process_seqs] Processed 729784 reads in 159.685 CPU sec, 5.155 real sec sorting block 46, #reads = 1091740 mem = 536877268 peak = 998248448 [M::process] read 729316 sequences (100000239 bp) in 3.363 CPU sec, 3.636 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 298773, 48, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 650, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3387) [M::mem_pestat] mean and std.dev: (617.45, 540.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (202.31, 96.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 349, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2185) [M::mem_pestat] mean and std.dev: (422.84, 406.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2863) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 574, 1171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3065) [M::mem_pestat] mean and std.dev: (650.78, 658.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 298962, 34, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 627, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2631) [M::mem_pestat] mean and std.dev: (662.69, 601.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.32, 97.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (180, 473, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (554.26, 508.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 376, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3471) [M::mem_pestat] mean and std.dev: (718.96, 720.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 727944 sequences (100000107 bp) in 3.376 CPU sec, 3.883 real sec... sorting block 47, #reads = 1094009 mem = 536879722 peak = 998248448 [M::mem_process_seqs] Processed 730412 reads in 219.668 CPU sec, 7.168 real sec [M::mem_process_seqs] Processed 729316 reads in 154.865 CPU sec, 5.048 real sec [M::process] read 727586 sequences (100000141 bp) in 3.552 CPU sec, 4.008 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 298794, 38, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 292, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (300.80, 169.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 185, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (205.85, 98.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 534, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3283) [M::mem_pestat] mean and std.dev: (879.44, 935.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4621) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 436, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2550) [M::mem_pestat] mean and std.dev: (584.20, 500.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 48, #reads = 1097821 mem = 536883003 peak = 998248448 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 298908, 42, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 435, 2321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6575) [M::mem_pestat] mean and std.dev: (908.75, 1061.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 186, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.27, 99.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (424, 805, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2701) [M::mem_pestat] mean and std.dev: (876.18, 696.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 310, 1103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2935) [M::mem_pestat] mean and std.dev: (612.20, 586.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 727214 sequences (100000011 bp) in 3.375 CPU sec, 3.825 real sec... [M::mem_process_seqs] Processed 727944 reads in 216.946 CPU sec, 7.125 real sec [M::mem_process_seqs] Processed 727586 reads in 154.732 CPU sec, 5.057 real sec sorting block 49, #reads = 1096389 mem = 536880485 peak = 998248448 [M::process] read 726468 sequences (100000099 bp) in 3.510 CPU sec, 3.975 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 298931, 49, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 291, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1971) [M::mem_pestat] mean and std.dev: (525.30, 518.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 186, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.47, 99.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (236, 544, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3401) [M::mem_pestat] mean and std.dev: (666.49, 605.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 395, 1651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4463) [M::mem_pestat] mean and std.dev: (737.22, 857.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 298126, 48, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 430, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2885) [M::mem_pestat] mean and std.dev: (671.35, 650.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 187, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (208.39, 100.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (121, 472, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3943) [M::mem_pestat] mean and std.dev: (722.85, 788.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 370, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3520) [M::mem_pestat] mean and std.dev: (684.00, 615.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4626) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 725790 sequences (100000032 bp) in 3.724 CPU sec, 4.415 real sec... [M::mem_process_seqs] Processed 727214 reads in 218.458 CPU sec, 7.145 real sec [M::mem_process_seqs] Processed 726468 reads in 153.100 CPU sec, 4.991 real sec sorting block 50, #reads = 1096065 mem = 536877030 peak = 998248448 [M::process] read 725384 sequences (100000215 bp) in 3.127 CPU sec, 3.384 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 298270, 41, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 337, 1581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4367) [M::mem_pestat] mean and std.dev: (917.71, 1004.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 188, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (209.56, 100.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 475, 1575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4299) [M::mem_pestat] mean and std.dev: (587.94, 617.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5661) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (368, 1180, 1879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4901) [M::mem_pestat] mean and std.dev: (892.55, 653.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6412) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 298153, 43, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 440, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137) [M::mem_pestat] mean and std.dev: (434.80, 330.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (210.18, 101.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (406, 851, 2227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5869) [M::mem_pestat] mean and std.dev: (1418.65, 1248.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7690) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 626, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3640) [M::mem_pestat] mean and std.dev: (815.94, 722.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724666 sequences (100000151 bp) in 3.335 CPU sec, 4.007 real sec... sorting block 51, #reads = 1096695 mem = 536878403 peak = 1008746496 [M::mem_process_seqs] Processed 725790 reads in 215.334 CPU sec, 7.070 real sec [M::mem_process_seqs] Processed 725384 reads in 150.830 CPU sec, 5.002 real sec [M::process] read 725114 sequences (100000077 bp) in 3.596 CPU sec, 4.030 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 297944, 36, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 474, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2297) [M::mem_pestat] mean and std.dev: (556.81, 443.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.29, 101.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 771, 1264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3168) [M::mem_pestat] mean and std.dev: (704.27, 588.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4120) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 330, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2214) [M::mem_pestat] mean and std.dev: (476.76, 447.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724666 reads in 124.174 CPU sec, 4.195 real sec sorting block 52, #reads = 1094043 mem = 536878446 peak = 1008746496 [M::process] read 724542 sequences (100000205 bp) in 2.978 CPU sec, 3.237 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 298077, 57, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 410, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859) [M::mem_pestat] mean and std.dev: (574.90, 478.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 189, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (211.42, 102.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 550, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2241) [M::mem_pestat] mean and std.dev: (603.72, 465.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2898) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 447, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2044) [M::mem_pestat] mean and std.dev: (464.19, 318.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 53, #reads = 1094432 mem = 536878162 peak = 1021157376 [M::process] read 724130 sequences (100000043 bp) in 2.895 CPU sec, 3.287 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 297733, 36, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 311, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614) [M::mem_pestat] mean and std.dev: (369.50, 306.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (211.87, 102.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (459, 1451, 3278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8916) [M::mem_pestat] mean and std.dev: (1771.82, 2026.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11735) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 518, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756) [M::mem_pestat] mean and std.dev: (673.14, 567.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725114 reads in 246.474 CPU sec, 8.256 real sec [M::mem_process_seqs] Processed 724542 reads in 153.895 CPU sec, 5.188 real sec [M::process] read 724228 sequences (100000291 bp) in 3.046 CPU sec, 3.286 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 297280, 47, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 235, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (286.08, 237.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (212.30, 102.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 478, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4075) [M::mem_pestat] mean and std.dev: (807.89, 709.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5360) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 325, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3753) [M::mem_pestat] mean and std.dev: (671.62, 741.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 54, #reads = 1094255 mem = 536881855 peak = 1021157376 [M::process] read 723888 sequences (100000053 bp) in 3.686 CPU sec, 4.095 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 297785, 40, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 506, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2010) [M::mem_pestat] mean and std.dev: (566.47, 438.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (212.79, 103.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (161, 295, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2585) [M::mem_pestat] mean and std.dev: (508.87, 478.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3393) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 341, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1920) [M::mem_pestat] mean and std.dev: (430.98, 347.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2504) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724130 reads in 213.321 CPU sec, 7.005 real sec [M::mem_process_seqs] Processed 724228 reads in 150.077 CPU sec, 4.912 real sec sorting block 55, #reads = 1094752 mem = 536883709 peak = 1021157376 [M::process] read 723440 sequences (100000194 bp) in 3.534 CPU sec, 3.889 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 297312, 42, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 360, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3018) [M::mem_pestat] mean and std.dev: (615.89, 561.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (213.34, 103.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (149, 480, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238) [M::mem_pestat] mean and std.dev: (365.76, 245.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 483, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2868) [M::mem_pestat] mean and std.dev: (718.47, 664.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 296429, 29, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 401, 1520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4252) [M::mem_pestat] mean and std.dev: (827.92, 917.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (213.52, 103.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 533, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3894) [M::mem_pestat] mean and std.dev: (737.39, 846.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 342, 1332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3570) [M::mem_pestat] mean and std.dev: (644.00, 680.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723958 sequences (100000095 bp) in 3.423 CPU sec, 4.095 real sec... [M::mem_process_seqs] Processed 723888 reads in 216.478 CPU sec, 7.106 real sec [M::mem_process_seqs] Processed 723440 reads in 153.363 CPU sec, 5.033 real sec sorting block 56, #reads = 1095027 mem = 536880776 peak = 1021157376 [M::process] read 723108 sequences (100000121 bp) in 3.379 CPU sec, 3.850 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 296872, 43, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 657, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3882) [M::mem_pestat] mean and std.dev: (788.56, 656.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.71, 101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 655, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3022) [M::mem_pestat] mean and std.dev: (730.05, 687.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3981) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 525, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4841) [M::mem_pestat] mean and std.dev: (1080.53, 1143.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6374) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723958 reads in 124.686 CPU sec, 4.376 real sec sorting block 57, #reads = 1093481 mem = 536881701 peak = 1021157376 [M::process] read 723336 sequences (100000122 bp) in 3.105 CPU sec, 3.535 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 296769, 39, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 373, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269) [M::mem_pestat] mean and std.dev: (640.81, 695.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.20, 101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (253, 852, 1431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3787) [M::mem_pestat] mean and std.dev: (850.11, 717.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 499, 1500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4094) [M::mem_pestat] mean and std.dev: (725.29, 736.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723332 sequences (100000223 bp) in 3.157 CPU sec, 3.705 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 297224, 50, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 451, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3724) [M::mem_pestat] mean and std.dev: (722.69, 779.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.97, 101.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 823, 1307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3609) [M::mem_pestat] mean and std.dev: (732.96, 619.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 322, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328) [M::mem_pestat] mean and std.dev: (445.04, 434.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723108 reads in 249.396 CPU sec, 8.372 real sec [M::mem_process_seqs] Processed 723336 reads in 152.873 CPU sec, 5.096 real sec sorting block 58, #reads = 1093544 mem = 536879822 peak = 1021157376 [M::process] read 723586 sequences (100000253 bp) in 3.591 CPU sec, 4.111 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 296709, 46, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 408, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2685) [M::mem_pestat] mean and std.dev: (642.09, 675.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.26, 101.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 512, 1298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3342) [M::mem_pestat] mean and std.dev: (726.18, 677.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 257, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (246.42, 94.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 297163, 52, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 389, 1600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4366) [M::mem_pestat] mean and std.dev: (806.43, 829.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.15, 101.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (545, 921, 1334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2912) [M::mem_pestat] mean and std.dev: (920.96, 645.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 307, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2723) [M::mem_pestat] mean and std.dev: (582.32, 638.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 59, #reads = 1093444 mem = 536879942 peak = 1021157376 [M::process] read 723276 sequences (100000198 bp) in 3.423 CPU sec, 4.028 real sec... [M::mem_process_seqs] Processed 723332 reads in 227.316 CPU sec, 7.508 real sec [M::mem_process_seqs] Processed 723586 reads in 150.329 CPU sec, 4.979 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 297078, 32, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 527, 1888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5178) [M::mem_pestat] mean and std.dev: (813.26, 805.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.81, 101.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 320, 1338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3780) [M::mem_pestat] mean and std.dev: (571.00, 645.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5001) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 375, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2798) [M::mem_pestat] mean and std.dev: (519.60, 579.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723684 sequences (100000173 bp) in 3.831 CPU sec, 4.361 real sec... [M::mem_process_seqs] Processed 723276 reads in 123.430 CPU sec, 4.192 real sec sorting block 60, #reads = 1093652 mem = 536878159 peak = 1021157376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 296878, 36, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 371, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2884) [M::mem_pestat] mean and std.dev: (609.24, 665.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.04, 101.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 504, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1932) [M::mem_pestat] mean and std.dev: (414.16, 302.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 384, 1173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3083) [M::mem_pestat] mean and std.dev: (652.02, 593.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723096 sequences (100000000 bp) in 3.667 CPU sec, 4.031 real sec... [M::mem_process_seqs] Processed 723684 reads in 124.748 CPU sec, 4.255 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 296827, 63, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 264, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2366) [M::mem_pestat] mean and std.dev: (497.16, 506.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (211.99, 101.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (405, 943, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3549) [M::mem_pestat] mean and std.dev: (937.45, 773.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 524, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2970) [M::mem_pestat] mean and std.dev: (679.69, 541.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 61, #reads = 1092913 mem = 536885553 peak = 1021157376 [M::process] read 723118 sequences (100000201 bp) in 3.427 CPU sec, 4.006 real sec... [M::mem_process_seqs] Processed 723096 reads in 123.188 CPU sec, 4.309 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 296482, 33, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 273, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2076) [M::mem_pestat] mean and std.dev: (362.33, 305.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.23, 102.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 393, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137) [M::mem_pestat] mean and std.dev: (510.37, 475.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 591, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3330) [M::mem_pestat] mean and std.dev: (726.52, 619.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 722700 sequences (100000148 bp) in 3.697 CPU sec, 4.064 real sec... [M::mem_process_seqs] Processed 723118 reads in 121.783 CPU sec, 4.386 real sec sorting block 62, #reads = 1092467 mem = 536878743 peak = 1021157376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 296721, 57, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 605, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2621) [M::mem_pestat] mean and std.dev: (736.38, 586.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.38, 101.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (219, 447, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2607) [M::mem_pestat] mean and std.dev: (715.05, 761.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 579, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2313) [M::mem_pestat] mean and std.dev: (551.18, 427.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 722374 sequences (100000069 bp) in 3.306 CPU sec, 3.655 real sec... [M::mem_process_seqs] Processed 722700 reads in 121.827 CPU sec, 4.349 real sec sorting block 63, #reads = 1093654 mem = 536881560 peak = 1021157376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 296396, 42, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 422, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3449) [M::mem_pestat] mean and std.dev: (676.78, 697.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.61, 101.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 749, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3570) [M::mem_pestat] mean and std.dev: (629.91, 678.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4702) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 310, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757) [M::mem_pestat] mean and std.dev: (411.50, 356.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 722368 sequences (100000054 bp) in 3.747 CPU sec, 4.128 real sec... [M::mem_process_seqs] Processed 722374 reads in 124.477 CPU sec, 4.260 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 296818, 33, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 280, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3421) [M::mem_pestat] mean and std.dev: (483.37, 509.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (212.53, 101.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 287, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4327) [M::mem_pestat] mean and std.dev: (639.55, 736.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 368, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2421) [M::mem_pestat] mean and std.dev: (482.64, 418.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 722824 sequences (100000275 bp) in 3.069 CPU sec, 3.585 real sec... [M::mem_process_seqs] Processed 722368 reads in 121.169 CPU sec, 4.200 real sec sorting block 64, #reads = 1093399 mem = 536881432 peak = 1021157376 [M::process] read 722456 sequences (100000212 bp) in 3.219 CPU sec, 3.597 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 296546, 46, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 529, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4033) [M::mem_pestat] mean and std.dev: (869.12, 1074.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.80, 102.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 792, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5138) [M::mem_pestat] mean and std.dev: (1070.43, 1154.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6788) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 487, 1111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2777) [M::mem_pestat] mean and std.dev: (645.85, 539.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 722824 reads in 124.436 CPU sec, 4.205 real sec [M::process] read 721456 sequences (100000238 bp) in 3.255 CPU sec, 3.607 real sec... sorting block 65, #reads = 1093815 mem = 536892087 peak = 1021157376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 296622, 47, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 492, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1679) [M::mem_pestat] mean and std.dev: (513.56, 359.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 192, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (213.81, 103.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 1125, 3771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10769) [M::mem_pestat] mean and std.dev: (2080.96, 2283.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 554, 1452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3900) [M::mem_pestat] mean and std.dev: (674.26, 681.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5124) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 296617, 19, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 372, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2401) [M::mem_pestat] mean and std.dev: (482.05, 458.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 192, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (214.61, 103.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 504, 1886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5422) [M::mem_pestat] mean and std.dev: (755.47, 962.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7190) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 326, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657) [M::mem_pestat] mean and std.dev: (433.63, 374.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 721470 sequences (100000049 bp) in 3.529 CPU sec, 4.163 real sec... [M::mem_process_seqs] Processed 722456 reads in 253.169 CPU sec, 8.507 real sec [M::mem_process_seqs] Processed 721456 reads in 153.192 CPU sec, 5.101 real sec sorting block 66, #reads = 1092484 mem = 536881016 peak = 1021157376 [M::process] read 720792 sequences (100000086 bp) in 2.837 CPU sec, 3.046 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 296155, 42, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 426, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2021) [M::mem_pestat] mean and std.dev: (562.73, 423.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 193, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (214.90, 103.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 552, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3853) [M::mem_pestat] mean and std.dev: (831.17, 871.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 474, 912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192) [M::mem_pestat] mean and std.dev: (588.68, 466.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 67, #reads = 1093028 mem = 536882880 peak = 1034948608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 296688, 28, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 412, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2456) [M::mem_pestat] mean and std.dev: (528.91, 406.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.71, 103.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (391, 924, 1627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4099) [M::mem_pestat] mean and std.dev: (1034.58, 874.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 532, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4243) [M::mem_pestat] mean and std.dev: (607.48, 651.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5585) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 720060 sequences (100000096 bp) in 3.501 CPU sec, 4.030 real sec... [M::mem_process_seqs] Processed 721470 reads in 214.256 CPU sec, 7.055 real sec [M::mem_process_seqs] Processed 720792 reads in 152.773 CPU sec, 5.067 real sec [M::process] read 720218 sequences (100000288 bp) in 3.527 CPU sec, 3.977 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 296174, 43, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 395, 861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2211) [M::mem_pestat] mean and std.dev: (533.81, 494.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (217.10, 104.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 169, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2060) [M::mem_pestat] mean and std.dev: (391.60, 427.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 391, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983) [M::mem_pestat] mean and std.dev: (486.61, 408.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 68, #reads = 1094067 mem = 536878271 peak = 1034948608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 296450, 32, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 543, 1671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4615) [M::mem_pestat] mean and std.dev: (1058.44, 1156.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 195, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (217.58, 105.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 461, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2549) [M::mem_pestat] mean and std.dev: (607.13, 541.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 381, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1471) [M::mem_pestat] mean and std.dev: (482.70, 351.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 727852 sequences (100000031 bp) in 3.338 CPU sec, 3.819 real sec... [M::mem_process_seqs] Processed 720060 reads in 219.738 CPU sec, 7.251 real sec [M::mem_process_seqs] Processed 720218 reads in 151.639 CPU sec, 5.037 real sec sorting block 69, #reads = 1092341 mem = 536883613 peak = 1034948608 [M::process] read 731428 sequences (100000288 bp) in 3.250 CPU sec, 3.564 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 298216, 42, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 250, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2213) [M::mem_pestat] mean and std.dev: (397.54, 356.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 185, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (205.94, 98.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 364, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150) [M::mem_pestat] mean and std.dev: (446.21, 390.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 258, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (338.00, 277.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 299444, 36, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 303, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861) [M::mem_pestat] mean and std.dev: (402.25, 285.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (200.92, 95.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (257, 716, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020) [M::mem_pestat] mean and std.dev: (651.43, 505.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 377, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1865) [M::mem_pestat] mean and std.dev: (464.45, 380.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2406) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 730290 sequences (100000141 bp) in 3.339 CPU sec, 3.882 real sec... [M::mem_process_seqs] Processed 727852 reads in 221.305 CPU sec, 7.208 real sec [M::mem_process_seqs] Processed 731428 reads in 153.596 CPU sec, 4.971 real sec sorting block 70, #reads = 1091593 mem = 536877130 peak = 1034948608 [M::process] read 729474 sequences (100000048 bp) in 3.552 CPU sec, 3.880 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 299682, 50, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 371, 2872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8262) [M::mem_pestat] mean and std.dev: (1005.06, 1186.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (202.41, 96.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (207, 360, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2376) [M::mem_pestat] mean and std.dev: (566.52, 466.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3099) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 646, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2910) [M::mem_pestat] mean and std.dev: (706.00, 501.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 730290 reads in 122.564 CPU sec, 4.187 real sec [M::process] read 728592 sequences (100000156 bp) in 3.408 CPU sec, 4.014 real sec... sorting block 71, #reads = 1098506 mem = 536881892 peak = 1034948608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 298857, 50, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 482, 1399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3835) [M::mem_pestat] mean and std.dev: (820.98, 875.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 183, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (203.34, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (219, 660, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2355) [M::mem_pestat] mean and std.dev: (641.64, 557.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 361, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2853) [M::mem_pestat] mean and std.dev: (655.67, 745.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3738) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 729474 reads in 152.355 CPU sec, 5.217 real sec [M::process] read 728626 sequences (100000157 bp) in 3.210 CPU sec, 3.627 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 299048, 46, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 423, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3909) [M::mem_pestat] mean and std.dev: (833.11, 861.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.59, 97.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 513, 1466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4070) [M::mem_pestat] mean and std.dev: (894.43, 993.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 359, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3395) [M::mem_pestat] mean and std.dev: (567.61, 551.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 72, #reads = 1098322 mem = 536885448 peak = 1034948608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 298768, 43, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 455, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (533.17, 395.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (204.93, 98.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (137, 570, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4352) [M::mem_pestat] mean and std.dev: (649.56, 852.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5757) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 410, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2498) [M::mem_pestat] mean and std.dev: (685.87, 626.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 728098 sequences (100000202 bp) in 3.333 CPU sec, 3.730 real sec... [M::mem_process_seqs] Processed 728592 reads in 244.212 CPU sec, 8.032 real sec [M::mem_process_seqs] Processed 728626 reads in 150.305 CPU sec, 4.843 real sec sorting block 73, #reads = 1097695 mem = 536880241 peak = 1034948608 [M::process] read 727990 sequences (100000265 bp) in 3.556 CPU sec, 3.882 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 298601, 36, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 399, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4177) [M::mem_pestat] mean and std.dev: (702.08, 727.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 185, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (206.26, 99.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 279, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2534) [M::mem_pestat] mean and std.dev: (495.51, 539.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 386, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2374) [M::mem_pestat] mean and std.dev: (562.42, 482.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3100) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 298977, 34, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 521, 1066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2832) [M::mem_pestat] mean and std.dev: (710.35, 651.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (206.12, 99.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (462, 672, 1799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4473) [M::mem_pestat] mean and std.dev: (968.81, 954.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 830, 1595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4239) [M::mem_pestat] mean and std.dev: (859.98, 661.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 727032 sequences (100000085 bp) in 2.744 CPU sec, 3.142 real sec... [M::mem_process_seqs] Processed 728098 reads in 216.170 CPU sec, 7.134 real sec [M::mem_process_seqs] Processed 727990 reads in 149.485 CPU sec, 4.938 real sec sorting block 74, #reads = 1097733 mem = 536877817 peak = 1034948608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 298524, 36, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 546, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2385) [M::mem_pestat] mean and std.dev: (630.21, 526.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 186, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (207.97, 100.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 621, 1854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5096) [M::mem_pestat] mean and std.dev: (949.16, 1051.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 486, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3012) [M::mem_pestat] mean and std.dev: (627.43, 638.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 726156 sequences (100000013 bp) in 4.003 CPU sec, 4.524 real sec... [M::mem_process_seqs] Processed 727032 reads in 124.418 CPU sec, 4.187 real sec sorting block 75, #reads = 1097093 mem = 536879373 peak = 1034948608 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 298249, 35, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 338, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3477) [M::mem_pestat] mean and std.dev: (626.47, 730.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (208.49, 100.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (137, 503, 4611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13559) [M::mem_pestat] mean and std.dev: (1775.54, 2322.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 434, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2456) [M::mem_pestat] mean and std.dev: (599.07, 468.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 726038 sequences (100000111 bp) in 3.489 CPU sec, 3.842 real sec... [M::process] read 725420 sequences (100000140 bp) in 3.087 CPU sec, 3.508 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 298522, 56, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 391, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2520) [M::mem_pestat] mean and std.dev: (501.90, 468.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (209.12, 101.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 684, 1614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4296) [M::mem_pestat] mean and std.dev: (1009.79, 1040.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 316, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1955) [M::mem_pestat] mean and std.dev: (427.91, 329.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726038 reads in 119.793 CPU sec, 4.023 real sec sorting block 76, #reads = 1097020 mem = 536886677 peak = 1034948608 [M::process] read 727226 sequences (100000019 bp) in 3.129 CPU sec, 3.301 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 297859, 38, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 536, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4522) [M::mem_pestat] mean and std.dev: (806.36, 838.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 188, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (209.87, 101.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 380, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2762) [M::mem_pestat] mean and std.dev: (572.64, 610.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 430, 1262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3414) [M::mem_pestat] mean and std.dev: (873.02, 989.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726156 reads in 338.940 CPU sec, 11.394 real sec [M::mem_process_seqs] Processed 725420 reads in 152.849 CPU sec, 5.147 real sec sorting block 77, #reads = 1095507 mem = 536881038 peak = 1047678976 [M::process] read 724940 sequences (100000279 bp) in 2.875 CPU sec, 3.508 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 298938, 25, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 456, 1455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3705) [M::mem_pestat] mean and std.dev: (808.27, 828.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 185, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.72, 99.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 497, 2158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6066) [M::mem_pestat] mean and std.dev: (1208.36, 1399.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 526, 1162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2920) [M::mem_pestat] mean and std.dev: (595.97, 481.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 297769, 38, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 455, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2090) [M::mem_pestat] mean and std.dev: (560.87, 477.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.32, 102.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 576, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2725) [M::mem_pestat] mean and std.dev: (638.57, 464.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3546) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 411, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2486) [M::mem_pestat] mean and std.dev: (529.24, 473.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724684 sequences (100000047 bp) in 3.456 CPU sec, 3.978 real sec... [M::mem_process_seqs] Processed 727226 reads in 253.933 CPU sec, 8.470 real sec [M::mem_process_seqs] Processed 724940 reads in 150.990 CPU sec, 4.967 real sec sorting block 78, #reads = 1095670 mem = 536879428 peak = 1047678976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 297785, 32, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 298, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (276.38, 129.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.33, 102.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (230, 779, 1225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3215) [M::mem_pestat] mean and std.dev: (711.89, 546.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 380, 1698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4648) [M::mem_pestat] mean and std.dev: (874.08, 874.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724822 sequences (100000236 bp) in 4.042 CPU sec, 4.515 real sec... [M::mem_process_seqs] Processed 724684 reads in 121.418 CPU sec, 4.154 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 297537, 27, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 650, 1138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2890) [M::mem_pestat] mean and std.dev: (702.26, 571.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (210.79, 102.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (106, 475, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2491) [M::mem_pestat] mean and std.dev: (545.44, 528.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 583, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3652) [M::mem_pestat] mean and std.dev: (757.48, 611.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 79, #reads = 1096186 mem = 536879233 peak = 1047678976 [M::process] read 724546 sequences (100000104 bp) in 3.059 CPU sec, 3.440 real sec... [M::mem_process_seqs] Processed 724822 reads in 122.535 CPU sec, 4.190 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 297725, 46, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 335, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3093) [M::mem_pestat] mean and std.dev: (611.10, 581.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (210.90, 101.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 582, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3843) [M::mem_pestat] mean and std.dev: (852.98, 906.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (313, 809, 1590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4144) [M::mem_pestat] mean and std.dev: (1031.86, 909.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723772 sequences (100000260 bp) in 3.363 CPU sec, 3.739 real sec... [M::mem_process_seqs] Processed 724546 reads in 122.178 CPU sec, 4.127 real sec sorting block 80, #reads = 1094648 mem = 536879336 peak = 1047678976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 297413, 40, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 405, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1964) [M::mem_pestat] mean and std.dev: (462.15, 340.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.78, 102.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (107, 471, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4322) [M::mem_pestat] mean and std.dev: (669.95, 666.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 456, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3496) [M::mem_pestat] mean and std.dev: (582.55, 693.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723510 sequences (100000213 bp) in 3.144 CPU sec, 3.670 real sec... [M::mem_process_seqs] Processed 723772 reads in 119.541 CPU sec, 4.136 real sec sorting block 81, #reads = 1095285 mem = 536879165 peak = 1047678976 [M::process] read 723272 sequences (100000171 bp) in 3.270 CPU sec, 3.543 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 297047, 41, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 340, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2308) [M::mem_pestat] mean and std.dev: (572.48, 592.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.11, 102.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (189, 307, 1841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5145) [M::mem_pestat] mean and std.dev: (791.33, 1042.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 361, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1798) [M::mem_pestat] mean and std.dev: (464.31, 385.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723510 reads in 124.525 CPU sec, 4.153 real sec [M::process] read 723478 sequences (100000257 bp) in 3.329 CPU sec, 3.771 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 296682, 35, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 561, 1890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5144) [M::mem_pestat] mean and std.dev: (940.49, 1108.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.12, 100.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (163, 572, 1480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4114) [M::mem_pestat] mean and std.dev: (1006.03, 1254.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6026) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 277, 1779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4855) [M::mem_pestat] mean and std.dev: (778.72, 942.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723272 reads in 152.766 CPU sec, 5.181 real sec sorting block 82, #reads = 1094099 mem = 536878485 peak = 1047678976 [M::process] read 723730 sequences (100000105 bp) in 3.687 CPU sec, 4.004 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 297167, 53, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 394, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2118) [M::mem_pestat] mean and std.dev: (578.91, 487.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.35, 101.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 537, 1883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5227) [M::mem_pestat] mean and std.dev: (1119.56, 1295.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 541, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3378) [M::mem_pestat] mean and std.dev: (802.71, 740.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 83, #reads = 1093124 mem = 536879267 peak = 1047678976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 297008, 45, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 565, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3069) [M::mem_pestat] mean and std.dev: (838.89, 824.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.23, 101.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (215, 533, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4490) [M::mem_pestat] mean and std.dev: (889.19, 965.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5915) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 391, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1735) [M::mem_pestat] mean and std.dev: (492.69, 387.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723448 sequences (100000059 bp) in 3.377 CPU sec, 3.602 real sec... [M::mem_process_seqs] Processed 723478 reads in 240.081 CPU sec, 7.805 real sec [M::mem_process_seqs] Processed 723730 reads in 140.539 CPU sec, 4.478 real sec sorting block 84, #reads = 1093357 mem = 536879341 peak = 1047678976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 296781, 55, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 596, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2270) [M::mem_pestat] mean and std.dev: (538.07, 436.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.32, 101.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (236, 512, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3062) [M::mem_pestat] mean and std.dev: (742.52, 695.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4004) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 529, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3876) [M::mem_pestat] mean and std.dev: (867.22, 886.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 722900 sequences (100000035 bp) in 3.968 CPU sec, 4.173 real sec... [M::process] read 722704 sequences (100000116 bp) in 2.158 CPU sec, 2.174 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 297231, 46, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131) [M::mem_pestat] mean and std.dev: (444.77, 372.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (212.12, 101.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 754, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4867) [M::mem_pestat] mean and std.dev: (920.67, 842.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6420) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 455, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2650) [M::mem_pestat] mean and std.dev: (628.75, 627.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3471) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 722900 reads in 115.394 CPU sec, 3.623 real sec [M::process] read 722592 sequences (100000051 bp) in 2.246 CPU sec, 2.266 real sec... sorting block 85, #reads = 1093330 mem = 536875777 peak = 1047678976 [M::mem_process_seqs] Processed 723448 reads in 287.441 CPU sec, 9.234 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 296592, 32, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 327, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3958) [M::mem_pestat] mean and std.dev: (777.65, 808.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (212.18, 101.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 719, 2769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8149) [M::mem_pestat] mean and std.dev: (1354.32, 1803.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 381, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1936) [M::mem_pestat] mean and std.dev: (417.88, 306.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2499) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 296085, 27, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 436, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2008) [M::mem_pestat] mean and std.dev: (437.22, 353.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.17, 101.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (203, 394, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418) [M::mem_pestat] mean and std.dev: (351.14, 215.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 352, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2711) [M::mem_pestat] mean and std.dev: (593.71, 455.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 722446 sequences (100000013 bp) in 3.513 CPU sec, 4.580 real sec... [M::mem_process_seqs] Processed 722704 reads in 242.089 CPU sec, 7.770 real sec [M::mem_process_seqs] Processed 722592 reads in 151.451 CPU sec, 4.850 real sec sorting block 86, #reads = 1093587 mem = 536885579 peak = 1047678976 [M::process] read 722152 sequences (100000259 bp) in 4.094 CPU sec, 4.424 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 297262, 62, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 368, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2626) [M::mem_pestat] mean and std.dev: (519.00, 418.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 191, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.03, 102.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (248, 730, 1965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5399) [M::mem_pestat] mean and std.dev: (1332.82, 1479.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 453, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2947) [M::mem_pestat] mean and std.dev: (601.92, 633.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 722446 reads in 122.102 CPU sec, 4.072 real sec sorting block 87, #reads = 1093218 mem = 536891127 peak = 1047678976 [M::process] read 722068 sequences (100000057 bp) in 3.456 CPU sec, 4.205 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 296609, 46, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 345, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3262) [M::mem_pestat] mean and std.dev: (723.38, 690.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 192, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.29, 102.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (361, 679, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2728) [M::mem_pestat] mean and std.dev: (758.89, 514.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 374, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976) [M::mem_pestat] mean and std.dev: (526.55, 539.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 722152 reads in 151.346 CPU sec, 5.140 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 296912, 46, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 390, 1487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4183) [M::mem_pestat] mean and std.dev: (895.46, 1084.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5531) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 88, #reads = 1093168 mem = 536882343 peak = 1047678976 [M::mem_pestat] (25, 50, 75) percentile: (135, 192, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.35, 102.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (231, 601, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3411) [M::mem_pestat] mean and std.dev: (737.86, 626.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 355, 1020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2656) [M::mem_pestat] mean and std.dev: (624.86, 622.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 721616 sequences (100000075 bp) in 3.895 CPU sec, 4.286 real sec... [M::mem_process_seqs] Processed 722068 reads in 148.930 CPU sec, 4.997 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 296432, 35, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 654, 1791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4771) [M::mem_pestat] mean and std.dev: (968.88, 988.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 193, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.52, 101.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (88, 404, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2791) [M::mem_pestat] mean and std.dev: (410.90, 421.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 342, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2897) [M::mem_pestat] mean and std.dev: (626.53, 653.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 721082 sequences (100000017 bp) in 3.545 CPU sec, 3.846 real sec... [M::mem_process_seqs] Processed 721616 reads in 121.517 CPU sec, 4.011 real sec sorting block 89, #reads = 1092418 mem = 536886147 peak = 1047678976 [M::process] read 720702 sequences (100000145 bp) in 3.167 CPU sec, 3.363 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 295985, 28, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 474, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756) [M::mem_pestat] mean and std.dev: (579.88, 505.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (214.66, 102.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 642, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2890) [M::mem_pestat] mean and std.dev: (681.52, 545.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (291, 672, 2057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5589) [M::mem_pestat] mean and std.dev: (1245.33, 1226.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 720752 sequences (100000098 bp) in 2.126 CPU sec, 2.169 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 295994, 50, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 288, 1780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4964) [M::mem_pestat] mean and std.dev: (977.95, 1213.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 193, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (214.87, 102.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 402, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005) [M::mem_pestat] mean and std.dev: (432.11, 372.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 321, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2074) [M::mem_pestat] mean and std.dev: (489.48, 424.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 721082 reads in 197.381 CPU sec, 6.266 real sec [M::process] read 720420 sequences (100000020 bp) in 1.707 CPU sec, 1.710 real sec... sorting block 90, #reads = 1092482 mem = 536876593 peak = 1047678976 [M::mem_process_seqs] Processed 720702 reads in 137.446 CPU sec, 4.351 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 296211, 35, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 425, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844) [M::mem_pestat] mean and std.dev: (490.21, 417.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.95, 103.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 344, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2359) [M::mem_pestat] mean and std.dev: (491.00, 468.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 319, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1465) [M::mem_pestat] mean and std.dev: (333.92, 238.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 91, #reads = 1091665 mem = 536881083 peak = 1047678976 [M::process] read 720736 sequences (100000043 bp) in 3.673 CPU sec, 3.902 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 296383, 45, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 447, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1797) [M::mem_pestat] mean and std.dev: (484.95, 368.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 195, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (216.69, 104.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 606, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3572) [M::mem_pestat] mean and std.dev: (847.14, 793.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 386, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2177) [M::mem_pestat] mean and std.dev: (468.24, 283.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 720752 reads in 211.908 CPU sec, 6.786 real sec [M::mem_process_seqs] Processed 720420 reads in 151.909 CPU sec, 4.826 real sec [M::process] read 730286 sequences (100000164 bp) in 2.991 CPU sec, 3.086 real sec... sorting block 92, #reads = 1091042 mem = 536881247 peak = 1047678976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 296587, 48, 98) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 283, 841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2161) [M::mem_pestat] mean and std.dev: (490.88, 450.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (216.26, 104.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (323, 672, 2087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5615) [M::mem_pestat] mean and std.dev: (1099.35, 1040.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 370, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2347) [M::mem_pestat] mean and std.dev: (455.62, 388.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 735790 sequences (100000289 bp) in 2.871 CPU sec, 3.077 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 299294, 39, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 323, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3053) [M::mem_pestat] mean and std.dev: (549.64, 515.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (202.29, 96.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (468, 824, 2161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5547) [M::mem_pestat] mean and std.dev: (1176.20, 1118.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 635, 1534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4128) [M::mem_pestat] mean and std.dev: (852.22, 745.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 734372 sequences (100000217 bp) in 1.700 CPU sec, 1.696 real sec... [M::mem_process_seqs] Processed 720736 reads in 203.497 CPU sec, 6.490 real sec sorting block 93, #reads = 1091061 mem = 536890684 peak = 1047678976 [M::mem_process_seqs] Processed 730286 reads in 149.631 CPU sec, 4.774 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 301989, 50, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 446, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2002) [M::mem_pestat] mean and std.dev: (545.25, 496.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (193.60, 90.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 444, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2471) [M::mem_pestat] mean and std.dev: (599.52, 441.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 411, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2141) [M::mem_pestat] mean and std.dev: (478.32, 427.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 733200 sequences (100000021 bp) in 2.841 CPU sec, 2.951 real sec... [M::process] read 732004 sequences (100000097 bp) in 1.760 CPU sec, 1.756 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 301104, 45, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 371, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2406) [M::mem_pestat] mean and std.dev: (556.64, 479.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (195.54, 92.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (105, 424, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2376) [M::mem_pestat] mean and std.dev: (428.71, 410.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 352, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4699) [M::mem_pestat] mean and std.dev: (1057.61, 1264.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 735790 reads in 192.279 CPU sec, 6.098 real sec sorting block 94, #reads = 1093859 mem = 536879225 peak = 1047678976 [M::process] read 731298 sequences (100000250 bp) in 2.389 CPU sec, 2.393 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 301022, 32, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 279, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547) [M::mem_pestat] mean and std.dev: (444.31, 357.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (197.42, 93.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 490, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3151) [M::mem_pestat] mean and std.dev: (654.03, 595.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 376, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1468) [M::mem_pestat] mean and std.dev: (393.83, 253.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 733200 reads in 105.400 CPU sec, 3.606 real sec sorting block 95, #reads = 1100130 mem = 536879585 peak = 1075654656 [M::mem_process_seqs] Processed 734372 reads in 257.293 CPU sec, 8.437 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 300490, 39, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 619, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4522) [M::mem_pestat] mean and std.dev: (752.98, 789.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (198.81, 94.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 448, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3208) [M::mem_pestat] mean and std.dev: (737.25, 818.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 299, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2721) [M::mem_pestat] mean and std.dev: (490.87, 509.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 730318 sequences (100000046 bp) in 2.760 CPU sec, 2.825 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 300294, 29, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 297, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3365) [M::mem_pestat] mean and std.dev: (508.91, 579.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (200.00, 95.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (372, 634, 2113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5595) [M::mem_pestat] mean and std.dev: (1175.34, 1121.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 492, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2468) [M::mem_pestat] mean and std.dev: (680.54, 599.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 730080 sequences (100000130 bp) in 2.320 CPU sec, 2.321 real sec... [M::mem_process_seqs] Processed 732004 reads in 221.845 CPU sec, 7.065 real sec sorting block 96, #reads = 1099580 mem = 536882165 peak = 1075654656 [M::mem_process_seqs] Processed 731298 reads in 140.412 CPU sec, 4.442 real sec sorting block 97, #reads = 1099690 mem = 536878665 peak = 1106149376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 300211, 50, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 311, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2752) [M::mem_pestat] mean and std.dev: (579.37, 602.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (201.81, 96.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 686, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937) [M::mem_pestat] mean and std.dev: (567.64, 302.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2454) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 334, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2510) [M::mem_pestat] mean and std.dev: (427.83, 408.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 729456 sequences (100000161 bp) in 3.214 CPU sec, 3.356 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 300428, 38, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 411, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3611) [M::mem_pestat] mean and std.dev: (679.57, 709.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (202.75, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (341, 738, 1522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3884) [M::mem_pestat] mean and std.dev: (832.32, 704.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 261, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2040) [M::mem_pestat] mean and std.dev: (450.86, 451.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 728548 sequences (100000158 bp) in 2.551 CPU sec, 2.572 real sec... [M::mem_process_seqs] Processed 730318 reads in 210.359 CPU sec, 6.783 real sec [M::mem_process_seqs] Processed 730080 reads in 143.827 CPU sec, 4.687 real sec sorting block 98, #reads = 1097452 mem = 536884557 peak = 1106149376 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 299387, 46, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 435, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2814) [M::mem_pestat] mean and std.dev: (618.18, 628.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 182, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (203.47, 97.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (161, 619, 1684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4730) [M::mem_pestat] mean and std.dev: (831.55, 1043.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (323, 499, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2540) [M::mem_pestat] mean and std.dev: (691.74, 558.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3279) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 728218 sequences (100000100 bp) in 3.052 CPU sec, 3.162 real sec... [M::process] read 727596 sequences (100000273 bp) in 2.380 CPU sec, 2.381 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 299760, 30, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 302, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (343.40, 226.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 183, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.39, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 514, 2881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8279) [M::mem_pestat] mean and std.dev: (1334.90, 1427.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 509, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3886) [M::mem_pestat] mean and std.dev: (657.49, 631.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 729456 reads in 222.772 CPU sec, 7.166 real sec sorting block 99, #reads = 1094456 mem = 536885441 peak = 1106149376 [M::mem_process_seqs] Processed 728548 reads in 143.269 CPU sec, 4.677 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 298966, 42, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1410) [M::mem_pestat] mean and std.dev: (336.76, 267.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (205.88, 99.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 1483, 2717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7599) [M::mem_pestat] mean and std.dev: (1695.20, 1592.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 285, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (332.38, 205.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 727020 sequences (100000088 bp) in 3.380 CPU sec, 3.534 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 299110, 46, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 290, 1257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3409) [M::mem_pestat] mean and std.dev: (695.88, 754.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 184, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.74, 100.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 717, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3457) [M::mem_pestat] mean and std.dev: (724.40, 623.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 464, 1307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3451) [M::mem_pestat] mean and std.dev: (567.97, 532.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 727078 sequences (100000129 bp) in 2.289 CPU sec, 2.292 real sec... [M::mem_process_seqs] Processed 728218 reads in 210.985 CPU sec, 6.825 real sec sorting block 100, #reads = 1095707 mem = 536882775 peak = 1106149376 [M::mem_process_seqs] Processed 727596 reads in 144.576 CPU sec, 4.718 real sec sorting block 101, #reads = 1096799 mem = 536889736 peak = 1106149376 [M::process] read 726690 sequences (100000168 bp) in 2.937 CPU sec, 3.058 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 298573, 52, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 494, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3724) [M::mem_pestat] mean and std.dev: (663.96, 517.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 185, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.93, 100.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 567, 2018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5716) [M::mem_pestat] mean and std.dev: (1088.59, 1425.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 337, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3402) [M::mem_pestat] mean and std.dev: (766.96, 818.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 725870 sequences (100000115 bp) in 2.250 CPU sec, 2.257 real sec... [M::mem_process_seqs] Processed 727078 reads in 122.388 CPU sec, 3.889 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 298852, 62, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 379, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959) [M::mem_pestat] mean and std.dev: (507.83, 405.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 185, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.35, 100.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 741, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3163) [M::mem_pestat] mean and std.dev: (781.76, 631.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 432, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3173) [M::mem_pestat] mean and std.dev: (672.93, 731.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 298387, 48, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 444, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3820) [M::mem_pestat] mean and std.dev: (676.92, 740.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 186, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (208.24, 101.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (198, 564, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (527.48, 424.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 457, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2526) [M::mem_pestat] mean and std.dev: (576.28, 521.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727020 reads in 323.584 CPU sec, 10.387 real sec [M::process] read 725280 sequences (100000245 bp) in 2.688 CPU sec, 2.696 real sec... sorting block 102, #reads = 1094770 mem = 536879706 peak = 1106149376 [M::mem_process_seqs] Processed 726690 reads in 174.287 CPU sec, 5.822 real sec sorting block 103, #reads = 1094663 mem = 536883677 peak = 1110777856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 297969, 55, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 346, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2746) [M::mem_pestat] mean and std.dev: (547.31, 505.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (208.84, 101.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 319, 1190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3242) [M::mem_pestat] mean and std.dev: (551.66, 635.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 315, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2068) [M::mem_pestat] mean and std.dev: (443.06, 407.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 725186 sequences (100000243 bp) in 3.327 CPU sec, 3.499 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 297387, 28, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 416, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193) [M::mem_pestat] mean and std.dev: (566.69, 512.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (209.45, 101.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (308, 569, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2627) [M::mem_pestat] mean and std.dev: (682.62, 519.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 387, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061) [M::mem_pestat] mean and std.dev: (506.53, 437.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724618 sequences (100000067 bp) in 2.258 CPU sec, 2.258 real sec... [M::mem_process_seqs] Processed 725870 reads in 214.675 CPU sec, 7.062 real sec [M::mem_process_seqs] Processed 725280 reads in 149.448 CPU sec, 4.777 real sec sorting block 104, #reads = 1092269 mem = 536878794 peak = 1110777856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 297817, 42, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 340, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2217) [M::mem_pestat] mean and std.dev: (487.04, 410.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (209.60, 101.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 814, 1435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3497) [M::mem_pestat] mean and std.dev: (924.61, 723.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4528) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 319, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477) [M::mem_pestat] mean and std.dev: (385.39, 247.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724308 sequences (100000163 bp) in 3.806 CPU sec, 4.058 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 297303, 41, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 429, 1988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5432) [M::mem_pestat] mean and std.dev: (952.51, 1127.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (209.66, 101.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (113, 542, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3740) [M::mem_pestat] mean and std.dev: (754.00, 954.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 367, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2082) [M::mem_pestat] mean and std.dev: (466.67, 415.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2714) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725186 reads in 210.311 CPU sec, 6.687 real sec [M::process] read 724362 sequences (100000069 bp) in 2.742 CPU sec, 2.759 real sec... [M::mem_process_seqs] Processed 724618 reads in 149.674 CPU sec, 4.932 real sec sorting block 105, #reads = 1093102 mem = 536882182 peak = 1110777856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 296717, 37, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 457, 1425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3749) [M::mem_pestat] mean and std.dev: (761.31, 720.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (210.79, 101.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 733, 2674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7438) [M::mem_pestat] mean and std.dev: (1228.94, 1316.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 502, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3187) [M::mem_pestat] mean and std.dev: (733.77, 715.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4165) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724054 sequences (100000222 bp) in 2.947 CPU sec, 3.099 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 297007, 33, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 230, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2182) [M::mem_pestat] mean and std.dev: (449.74, 470.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (210.02, 101.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 951, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5740) [M::mem_pestat] mean and std.dev: (908.77, 831.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 393, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1888) [M::mem_pestat] mean and std.dev: (464.50, 378.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724808 sequences (100000091 bp) in 2.523 CPU sec, 2.544 real sec... [M::mem_process_seqs] Processed 724308 reads in 221.640 CPU sec, 7.273 real sec sorting block 106, #reads = 1091669 mem = 536879816 peak = 1110777856 [M::mem_process_seqs] Processed 724362 reads in 151.664 CPU sec, 4.924 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 297179, 46, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 536, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2547) [M::mem_pestat] mean and std.dev: (632.03, 547.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.20, 101.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 671, 1805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4795) [M::mem_pestat] mean and std.dev: (994.21, 1071.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6290) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 1037, 2387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6477) [M::mem_pestat] mean and std.dev: (1458.32, 1418.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8522) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 107, #reads = 1092082 mem = 536878899 peak = 1110777856 [M::process] read 723854 sequences (100000172 bp) in 3.360 CPU sec, 3.519 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 297831, 55, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 443, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264) [M::mem_pestat] mean and std.dev: (667.06, 651.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (209.54, 101.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (290, 505, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2360) [M::mem_pestat] mean and std.dev: (608.78, 558.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3050) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 434, 1369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3607) [M::mem_pestat] mean and std.dev: (817.10, 840.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724054 reads in 216.851 CPU sec, 7.016 real sec [M::process] read 723496 sequences (100000169 bp) in 2.624 CPU sec, 2.635 real sec... [M::mem_process_seqs] Processed 724808 reads in 155.303 CPU sec, 5.177 real sec sorting block 108, #reads = 1091221 mem = 536880561 peak = 1110777856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 297093, 52, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 366, 1710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4660) [M::mem_pestat] mean and std.dev: (792.65, 912.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.47, 102.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (257, 534, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2453) [M::mem_pestat] mean and std.dev: (576.62, 441.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 386, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3021) [M::mem_pestat] mean and std.dev: (562.92, 545.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723540 sequences (100000178 bp) in 3.344 CPU sec, 3.507 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 296652, 45, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2693) [M::mem_pestat] mean and std.dev: (546.85, 658.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.55, 103.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 404, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2665) [M::mem_pestat] mean and std.dev: (436.62, 366.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 508, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2670) [M::mem_pestat] mean and std.dev: (652.74, 523.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 722972 sequences (100000060 bp) in 2.598 CPU sec, 2.603 real sec... [M::mem_process_seqs] Processed 723854 reads in 224.781 CPU sec, 7.406 real sec [M::mem_process_seqs] Processed 723496 reads in 155.038 CPU sec, 5.013 real sec sorting block 109, #reads = 1090239 mem = 536886555 peak = 1110777856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 297410, 48, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 319, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143) [M::mem_pestat] mean and std.dev: (436.05, 369.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.12, 101.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 514, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3152) [M::mem_pestat] mean and std.dev: (550.68, 554.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 333, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827) [M::mem_pestat] mean and std.dev: (478.14, 533.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723052 sequences (100000017 bp) in 3.552 CPU sec, 3.783 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 296930, 40, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 321, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2322) [M::mem_pestat] mean and std.dev: (483.60, 481.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.78, 101.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (332, 681, 1887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4997) [M::mem_pestat] mean and std.dev: (1090.18, 1175.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 322, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (419.47, 363.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723540 reads in 225.178 CPU sec, 7.282 real sec [M::process] read 722782 sequences (100000269 bp) in 2.561 CPU sec, 2.567 real sec... sorting block 110, #reads = 1090895 mem = 536880502 peak = 1110777856 [M::mem_process_seqs] Processed 722972 reads in 158.253 CPU sec, 5.246 real sec sorting block 111, #reads = 1091206 mem = 536884445 peak = 1110777856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 296556, 50, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 401, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2040) [M::mem_pestat] mean and std.dev: (476.75, 351.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.82, 101.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 606, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2914) [M::mem_pestat] mean and std.dev: (718.84, 546.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 411, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2224) [M::mem_pestat] mean and std.dev: (508.87, 444.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723398 sequences (100000284 bp) in 3.419 CPU sec, 3.597 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 297022, 34, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 380, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2655) [M::mem_pestat] mean and std.dev: (665.76, 639.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.12, 101.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (480, 852, 1520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3600) [M::mem_pestat] mean and std.dev: (875.50, 594.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 389, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (586.50, 532.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723296 sequences (100000058 bp) in 2.676 CPU sec, 2.689 real sec... [M::mem_process_seqs] Processed 723052 reads in 229.190 CPU sec, 7.582 real sec [M::mem_process_seqs] Processed 722782 reads in 158.595 CPU sec, 5.221 real sec sorting block 112, #reads = 1090773 mem = 536877520 peak = 1110777856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 297217, 34, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 389, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3242) [M::mem_pestat] mean and std.dev: (717.32, 681.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.77, 101.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 813, 1334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3380) [M::mem_pestat] mean and std.dev: (910.72, 918.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4583) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 280, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2073) [M::mem_pestat] mean and std.dev: (420.38, 376.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723302 sequences (100000238 bp) in 3.413 CPU sec, 3.720 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 296950, 56, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 588, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3528) [M::mem_pestat] mean and std.dev: (866.54, 741.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.62, 101.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 550, 1328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3508) [M::mem_pestat] mean and std.dev: (799.33, 714.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 442, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2352) [M::mem_pestat] mean and std.dev: (492.00, 315.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723398 reads in 227.363 CPU sec, 7.473 real sec [M::mem_process_seqs] Processed 723296 reads in 161.428 CPU sec, 5.177 real sec [M::process] read 724096 sequences (100000057 bp) in 3.583 CPU sec, 3.843 real sec... sorting block 113, #reads = 1091064 mem = 536876771 peak = 1110777856 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 296726, 19, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 349, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1596) [M::mem_pestat] mean and std.dev: (384.13, 256.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.58, 101.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 314, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2844) [M::mem_pestat] mean and std.dev: (641.79, 625.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 391, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1946) [M::mem_pestat] mean and std.dev: (529.66, 412.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 737170 sequences (100000171 bp) in 3.198 CPU sec, 3.355 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 297024, 37, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 465, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2404) [M::mem_pestat] mean and std.dev: (483.42, 418.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.69, 101.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 508, 1511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4085) [M::mem_pestat] mean and std.dev: (763.29, 906.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 443, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4755) [M::mem_pestat] mean and std.dev: (930.73, 1008.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723302 reads in 229.023 CPU sec, 7.367 real sec sorting block 114, #reads = 1091963 mem = 536877054 peak = 1110777856 [M::mem_process_seqs] Processed 724096 reads in 162.724 CPU sec, 5.167 real sec [M::process] read 737002 sequences (100000138 bp) in 3.019 CPU sec, 3.121 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 302079, 35, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 481, 1825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5099) [M::mem_pestat] mean and std.dev: (803.70, 970.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (191.81, 89.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 444, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3122) [M::mem_pestat] mean and std.dev: (611.84, 629.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 398, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1893) [M::mem_pestat] mean and std.dev: (561.76, 476.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 115, #reads = 1089668 mem = 536883378 peak = 1137336320 [M::process] read 734766 sequences (100000161 bp) in 3.437 CPU sec, 4.082 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 301839, 32, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 361, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2062) [M::mem_pestat] mean and std.dev: (500.51, 393.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (191.94, 89.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 630, 1801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5075) [M::mem_pestat] mean and std.dev: (894.66, 1103.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 539, 1698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4576) [M::mem_pestat] mean and std.dev: (1006.66, 1084.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 737170 reads in 239.134 CPU sec, 8.043 real sec [M::process] read 733236 sequences (100000118 bp) in 2.516 CPU sec, 2.910 real sec... [M::mem_process_seqs] Processed 737002 reads in 167.428 CPU sec, 5.871 real sec sorting block 116, #reads = 1093266 mem = 536882318 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 302023, 23, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 352, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1927) [M::mem_pestat] mean and std.dev: (396.86, 267.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (195.58, 91.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (243, 997, 2028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5598) [M::mem_pestat] mean and std.dev: (1196.52, 1192.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7383) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 287, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296) [M::mem_pestat] mean and std.dev: (344.52, 230.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1667) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 732410 sequences (100000024 bp) in 3.226 CPU sec, 3.949 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 300926, 36, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 429, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2542) [M::mem_pestat] mean and std.dev: (521.12, 465.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (197.24, 92.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 193, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2477) [M::mem_pestat] mean and std.dev: (385.09, 439.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 551, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4827) [M::mem_pestat] mean and std.dev: (951.67, 1063.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6361) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 734766 reads in 232.194 CPU sec, 7.964 real sec [M::process] read 731908 sequences (100000009 bp) in 2.798 CPU sec, 2.965 real sec... [M::mem_process_seqs] Processed 733236 reads in 167.559 CPU sec, 5.709 real sec sorting block 117, #reads = 1096193 mem = 536878133 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 300850, 46, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 317, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2000) [M::mem_pestat] mean and std.dev: (560.52, 482.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (199.32, 94.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 553, 1378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3780) [M::mem_pestat] mean and std.dev: (831.19, 920.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4981) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 348, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739) [M::mem_pestat] mean and std.dev: (457.14, 396.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 731436 sequences (100000169 bp) in 3.379 CPU sec, 3.746 real sec... sorting block 118, #reads = 1096065 mem = 536878971 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 300244, 48, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 469, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1922) [M::mem_pestat] mean and std.dev: (476.52, 385.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (199.23, 94.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (382, 558, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2317) [M::mem_pestat] mean and std.dev: (744.87, 621.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 357, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2523) [M::mem_pestat] mean and std.dev: (647.65, 628.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3295) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 732410 reads in 230.678 CPU sec, 7.809 real sec [M::process] read 731828 sequences (100000031 bp) in 2.767 CPU sec, 2.933 real sec... [M::mem_process_seqs] Processed 731908 reads in 168.142 CPU sec, 5.717 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 300451, 42, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 390, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2371) [M::mem_pestat] mean and std.dev: (512.58, 444.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (199.20, 94.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (326, 733, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2942) [M::mem_pestat] mean and std.dev: (913.65, 846.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 298, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1912) [M::mem_pestat] mean and std.dev: (431.17, 359.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 119, #reads = 1096630 mem = 536879813 peak = 1137336320 [M::process] read 731046 sequences (100000034 bp) in 3.240 CPU sec, 3.910 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 300345, 44, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 365, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2499) [M::mem_pestat] mean and std.dev: (512.22, 470.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (199.37, 94.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 537, 1852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5446) [M::mem_pestat] mean and std.dev: (741.38, 826.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 456, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505) [M::mem_pestat] mean and std.dev: (583.62, 525.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 731436 reads in 236.037 CPU sec, 7.854 real sec [M::mem_process_seqs] Processed 731828 reads in 169.168 CPU sec, 5.594 real sec [M::process] read 730198 sequences (100000184 bp) in 2.953 CPU sec, 3.380 real sec... sorting block 120, #reads = 1096170 mem = 536879420 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 300086, 47, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 321, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2561) [M::mem_pestat] mean and std.dev: (471.89, 519.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (200.30, 95.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 777, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3693) [M::mem_pestat] mean and std.dev: (944.93, 762.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4809) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1872) [M::mem_pestat] mean and std.dev: (324.56, 267.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 729608 sequences (100000013 bp) in 3.201 CPU sec, 3.641 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 300033, 39, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 473, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2717) [M::mem_pestat] mean and std.dev: (486.52, 438.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (202.16, 96.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 600, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618) [M::mem_pestat] mean and std.dev: (504.81, 386.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 560, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3458) [M::mem_pestat] mean and std.dev: (812.07, 759.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4541) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 731046 reads in 236.179 CPU sec, 7.990 real sec [M::process] read 729270 sequences (100000205 bp) in 2.885 CPU sec, 3.206 real sec... [M::mem_process_seqs] Processed 730198 reads in 164.345 CPU sec, 5.717 real sec sorting block 121, #reads = 1093461 mem = 536880516 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 299856, 43, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 321, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749) [M::mem_pestat] mean and std.dev: (573.18, 582.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 182, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (203.90, 98.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 534, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2773) [M::mem_pestat] mean and std.dev: (660.51, 560.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3599) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 435, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2427) [M::mem_pestat] mean and std.dev: (627.19, 561.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 728470 sequences (100000268 bp) in 3.522 CPU sec, 4.336 real sec... sorting block 122, #reads = 1093713 mem = 536881824 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 300419, 32, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 592, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3430) [M::mem_pestat] mean and std.dev: (893.97, 819.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 183, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (204.58, 98.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 610, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827) [M::mem_pestat] mean and std.dev: (706.19, 680.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 287, 997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2567) [M::mem_pestat] mean and std.dev: (671.84, 688.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 729608 reads in 225.096 CPU sec, 7.724 real sec [M::mem_process_seqs] Processed 729270 reads in 159.262 CPU sec, 5.552 real sec [M::process] read 728306 sequences (100000225 bp) in 2.847 CPU sec, 3.140 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 299290, 29, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 391, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2282) [M::mem_pestat] mean and std.dev: (453.29, 366.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.13, 98.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (320, 663, 1419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3617) [M::mem_pestat] mean and std.dev: (648.00, 565.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 465, 1444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3852) [M::mem_pestat] mean and std.dev: (777.50, 760.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 123, #reads = 1094927 mem = 536881210 peak = 1137336320 [M::process] read 727268 sequences (100000005 bp) in 3.284 CPU sec, 3.884 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 299296, 53, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 383, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611) [M::mem_pestat] mean and std.dev: (413.05, 272.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.58, 99.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (170, 564, 1473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4079) [M::mem_pestat] mean and std.dev: (867.32, 952.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1730) [M::mem_pestat] mean and std.dev: (427.71, 366.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728470 reads in 227.927 CPU sec, 7.845 real sec [M::mem_process_seqs] Processed 728306 reads in 161.153 CPU sec, 5.587 real sec [M::process] read 726714 sequences (100000174 bp) in 2.958 CPU sec, 3.298 real sec... sorting block 124, #reads = 1094416 mem = 536884799 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 298662, 43, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 230, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1291) [M::mem_pestat] mean and std.dev: (291.00, 229.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 185, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.24, 100.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (490, 786, 1440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3340) [M::mem_pestat] mean and std.dev: (888.38, 587.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4290) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 404, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2385) [M::mem_pestat] mean and std.dev: (502.26, 411.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 726248 sequences (100000162 bp) in 3.423 CPU sec, 4.238 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 298374, 44, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 361, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1899) [M::mem_pestat] mean and std.dev: (440.67, 329.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 186, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (208.09, 100.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 604, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3240) [M::mem_pestat] mean and std.dev: (610.61, 660.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 389, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2250) [M::mem_pestat] mean and std.dev: (614.32, 579.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2937) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727268 reads in 233.159 CPU sec, 8.075 real sec [M::mem_process_seqs] Processed 726714 reads in 164.320 CPU sec, 5.927 real sec sorting block 125, #reads = 1093837 mem = 536877204 peak = 1137336320 [M::process] read 725716 sequences (100000190 bp) in 2.966 CPU sec, 3.191 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 298274, 31, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 266, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957) [M::mem_pestat] mean and std.dev: (387.56, 398.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 186, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (208.58, 101.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 448, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3592) [M::mem_pestat] mean and std.dev: (725.14, 808.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4715) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 401, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2245) [M::mem_pestat] mean and std.dev: (598.31, 532.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 126, #reads = 1091643 mem = 536880789 peak = 1137336320 [M::process] read 725580 sequences (100000189 bp) in 3.378 CPU sec, 3.526 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 297458, 47, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 464, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3126) [M::mem_pestat] mean and std.dev: (663.75, 689.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (208.84, 101.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (107, 435, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345) [M::mem_pestat] mean and std.dev: (529.26, 541.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3091) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 503, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3048) [M::mem_pestat] mean and std.dev: (799.27, 746.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726248 reads in 217.269 CPU sec, 6.947 real sec [M::process] read 725342 sequences (100000237 bp) in 2.194 CPU sec, 2.208 real sec... [M::mem_process_seqs] Processed 725716 reads in 152.643 CPU sec, 5.070 real sec sorting block 127, #reads = 1091607 mem = 536878891 peak = 1137336320 [M::process] read 724814 sequences (100000269 bp) in 3.041 CPU sec, 3.222 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 297704, 49, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 306, 1824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5162) [M::mem_pestat] mean and std.dev: (733.50, 945.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (208.46, 100.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 537, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3458) [M::mem_pestat] mean and std.dev: (683.62, 779.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 318, 928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2304) [M::mem_pestat] mean and std.dev: (520.69, 475.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724032 sequences (100000074 bp) in 2.556 CPU sec, 2.565 real sec... [M::mem_process_seqs] Processed 725342 reads in 132.282 CPU sec, 4.212 real sec sorting block 128, #reads = 1091263 mem = 536880958 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 297733, 35, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 324, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (510.56, 486.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (208.80, 100.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 659, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1943) [M::mem_pestat] mean and std.dev: (691.61, 409.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 694, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3139) [M::mem_pestat] mean and std.dev: (789.82, 614.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 297154, 47, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 536, 1498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4000) [M::mem_pestat] mean and std.dev: (724.71, 685.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (209.40, 100.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 516, 1372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3598) [M::mem_pestat] mean and std.dev: (873.09, 967.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 423, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2391) [M::mem_pestat] mean and std.dev: (533.83, 419.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725580 reads in 341.704 CPU sec, 11.137 real sec [M::process] read 724130 sequences (100000038 bp) in 2.813 CPU sec, 2.875 real sec... [M::mem_process_seqs] Processed 724814 reads in 188.837 CPU sec, 6.086 real sec sorting block 129, #reads = 1090189 mem = 536883453 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 297383, 27, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 420, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005) [M::mem_pestat] mean and std.dev: (515.55, 383.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.40, 101.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 391, 2753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7963) [M::mem_pestat] mean and std.dev: (1232.31, 1682.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 467, 1059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2751) [M::mem_pestat] mean and std.dev: (603.32, 548.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723650 sequences (100000057 bp) in 3.016 CPU sec, 3.278 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 297544, 35, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 420, 1616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4400) [M::mem_pestat] mean and std.dev: (962.98, 1038.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.49, 101.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 220, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2724) [M::mem_pestat] mean and std.dev: (492.09, 580.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (306, 715, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3096) [M::mem_pestat] mean and std.dev: (795.44, 587.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4026) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724032 reads in 216.965 CPU sec, 7.103 real sec [M::process] read 724748 sequences (100000102 bp) in 2.542 CPU sec, 2.660 real sec... [M::mem_process_seqs] Processed 724130 reads in 152.851 CPU sec, 5.238 real sec sorting block 130, #reads = 1091335 mem = 536878280 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 297264, 44, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 275, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389) [M::mem_pestat] mean and std.dev: (382.76, 300.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.49, 101.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 568, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3341) [M::mem_pestat] mean and std.dev: (630.05, 590.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 500, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3236) [M::mem_pestat] mean and std.dev: (682.54, 636.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4253) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724252 sequences (100000201 bp) in 3.073 CPU sec, 3.532 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 297496, 44, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 384, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131) [M::mem_pestat] mean and std.dev: (476.10, 416.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (209.53, 101.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (136, 504, 1251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3481) [M::mem_pestat] mean and std.dev: (616.75, 719.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 420, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2020) [M::mem_pestat] mean and std.dev: (517.21, 390.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723982 sequences (100000259 bp) in 2.617 CPU sec, 2.629 real sec... [M::mem_process_seqs] Processed 723650 reads in 224.314 CPU sec, 7.591 real sec sorting block 131, #reads = 1091869 mem = 536878049 peak = 1137336320 [M::mem_process_seqs] Processed 724748 reads in 156.338 CPU sec, 5.125 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 297657, 40, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 594, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3034) [M::mem_pestat] mean and std.dev: (799.98, 676.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (210.00, 101.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 555, 1352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3684) [M::mem_pestat] mean and std.dev: (840.34, 967.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 426, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723) [M::mem_pestat] mean and std.dev: (472.61, 360.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723876 sequences (100000150 bp) in 3.415 CPU sec, 3.772 real sec... sorting block 132, #reads = 1091333 mem = 536878792 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 297191, 25, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 321, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2082) [M::mem_pestat] mean and std.dev: (456.28, 381.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (210.81, 101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (442, 1091, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4393) [M::mem_pestat] mean and std.dev: (816.62, 563.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 387, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2751) [M::mem_pestat] mean and std.dev: (651.78, 625.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724252 reads in 222.568 CPU sec, 7.281 real sec [M::mem_process_seqs] Processed 723982 reads in 159.623 CPU sec, 5.135 real sec [M::process] read 724358 sequences (100000002 bp) in 2.962 CPU sec, 3.179 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 297216, 42, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 455, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3005) [M::mem_pestat] mean and std.dev: (712.17, 646.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.21, 102.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 687, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2959) [M::mem_pestat] mean and std.dev: (711.75, 546.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3871) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 474, 1091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2709) [M::mem_pestat] mean and std.dev: (687.25, 616.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3518) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 133, #reads = 1091156 mem = 536879469 peak = 1137336320 [M::process] read 724668 sequences (100000246 bp) in 3.498 CPU sec, 3.895 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 297454, 52, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 660, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4263) [M::mem_pestat] mean and std.dev: (1108.88, 1064.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.19, 101.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (360, 512, 1496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3768) [M::mem_pestat] mean and std.dev: (714.15, 712.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4904) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (313, 922, 2024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5446) [M::mem_pestat] mean and std.dev: (1407.96, 1395.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723876 reads in 231.574 CPU sec, 7.677 real sec [M::mem_process_seqs] Processed 724358 reads in 165.604 CPU sec, 5.497 real sec [M::process] read 729146 sequences (100000121 bp) in 3.155 CPU sec, 3.408 real sec... sorting block 134, #reads = 1092067 mem = 536879112 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 296990, 24, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 351, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1899) [M::mem_pestat] mean and std.dev: (440.07, 343.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (208.94, 100.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 684, 1560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4010) [M::mem_pestat] mean and std.dev: (783.59, 623.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 382, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2529) [M::mem_pestat] mean and std.dev: (578.95, 612.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 734910 sequences (100000166 bp) in 3.477 CPU sec, 3.847 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 297664, 47, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 439, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2654) [M::mem_pestat] mean and std.dev: (671.23, 649.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (202.87, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 630, 2753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7791) [M::mem_pestat] mean and std.dev: (1545.98, 1814.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10310) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 433, 1476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3994) [M::mem_pestat] mean and std.dev: (754.78, 874.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5253) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 733342 sequences (100000091 bp) in 2.652 CPU sec, 2.673 real sec... [M::mem_process_seqs] Processed 724668 reads in 260.777 CPU sec, 8.404 real sec sorting block 135, #reads = 1091010 mem = 536879234 peak = 1137336320 [M::mem_process_seqs] Processed 729146 reads in 195.931 CPU sec, 6.359 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 301685, 45, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 580, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2597) [M::mem_pestat] mean and std.dev: (640.88, 584.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (194.20, 90.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 730, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4509) [M::mem_pestat] mean and std.dev: (1005.63, 933.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 396, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2372) [M::mem_pestat] mean and std.dev: (622.13, 574.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 136, #reads = 1082264 mem = 536881528 peak = 1137336320 [M::process] read 731662 sequences (100000109 bp) in 3.237 CPU sec, 3.538 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 301177, 33, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 766, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3575) [M::mem_pestat] mean and std.dev: (823.91, 563.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (197.19, 93.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 259, 1281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3699) [M::mem_pestat] mean and std.dev: (666.97, 805.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 374, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (518.66, 439.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 734910 reads in 228.654 CPU sec, 7.461 real sec [M::process] read 730850 sequences (100000047 bp) in 2.615 CPU sec, 2.664 real sec... [M::mem_process_seqs] Processed 733342 reads in 159.440 CPU sec, 5.363 real sec sorting block 137, #reads = 1095159 mem = 536887320 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 300519, 26, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 303, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2530) [M::mem_pestat] mean and std.dev: (475.69, 517.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (200.13, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 623, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4534) [M::mem_pestat] mean and std.dev: (855.67, 865.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5964) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (344, 617, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2672) [M::mem_pestat] mean and std.dev: (615.89, 403.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 730670 sequences (100000181 bp) in 3.633 CPU sec, 4.037 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 300577, 56, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 438, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3516) [M::mem_pestat] mean and std.dev: (687.80, 651.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (201.12, 95.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 585, 861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (503.90, 442.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 343, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2923) [M::mem_pestat] mean and std.dev: (636.00, 643.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 731662 reads in 224.344 CPU sec, 7.528 real sec [M::process] read 730166 sequences (100000109 bp) in 2.845 CPU sec, 2.902 real sec... [M::mem_process_seqs] Processed 730850 reads in 157.549 CPU sec, 5.191 real sec sorting block 138, #reads = 1098224 mem = 536879539 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 300877, 51, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 472, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3367) [M::mem_pestat] mean and std.dev: (653.46, 659.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 181, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (202.09, 96.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (161, 547, 1431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3971) [M::mem_pestat] mean and std.dev: (757.83, 740.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 371, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1390) [M::mem_pestat] mean and std.dev: (375.33, 214.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 729396 sequences (100000096 bp) in 3.352 CPU sec, 3.665 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 300179, 34, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 483, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1707) [M::mem_pestat] mean and std.dev: (441.10, 312.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (201.89, 96.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 438, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2513) [M::mem_pestat] mean and std.dev: (501.97, 406.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 320, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2194) [M::mem_pestat] mean and std.dev: (440.34, 377.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 730670 reads in 224.212 CPU sec, 7.445 real sec sorting block 139, #reads = 1098465 mem = 536883002 peak = 1137336320 [M::mem_process_seqs] Processed 730166 reads in 159.480 CPU sec, 5.116 real sec [M::process] read 729174 sequences (100000230 bp) in 3.084 CPU sec, 3.243 real sec... sorting block 140, #reads = 1097511 mem = 536881015 peak = 1137336320 [M::process] read 728542 sequences (100000249 bp) in 3.250 CPU sec, 3.572 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 300020, 43, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 455, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2565) [M::mem_pestat] mean and std.dev: (681.32, 589.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 182, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (203.87, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (161, 284, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976) [M::mem_pestat] mean and std.dev: (410.12, 365.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2581) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (290, 432, 2997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8411) [M::mem_pestat] mean and std.dev: (1438.57, 1829.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 729174 reads in 128.492 CPU sec, 4.075 real sec [M::process] read 728688 sequences (100000163 bp) in 2.834 CPU sec, 2.900 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 300057, 39, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 269, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1730) [M::mem_pestat] mean and std.dev: (351.58, 283.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 183, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.65, 97.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 450, 1572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4292) [M::mem_pestat] mean and std.dev: (887.41, 1020.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 387, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2281) [M::mem_pestat] mean and std.dev: (527.31, 475.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2964) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 299512, 29, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 429, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2347) [M::mem_pestat] mean and std.dev: (681.81, 584.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.24, 99.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 524, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2965) [M::mem_pestat] mean and std.dev: (564.40, 574.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3890) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 367, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152) [M::mem_pestat] mean and std.dev: (527.55, 476.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 729396 reads in 348.749 CPU sec, 11.232 real sec sorting block 141, #reads = 1095580 mem = 536881117 peak = 1137336320 [M::mem_process_seqs] Processed 728542 reads in 185.149 CPU sec, 5.934 real sec [M::process] read 728512 sequences (100000244 bp) in 3.226 CPU sec, 3.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 299750, 46, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 321, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2982) [M::mem_pestat] mean and std.dev: (629.98, 715.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 183, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.36, 98.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 906, 2123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6033) [M::mem_pestat] mean and std.dev: (1540.48, 1748.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 315, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5076) [M::mem_pestat] mean and std.dev: (879.28, 1128.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 142, #reads = 1097103 mem = 536878496 peak = 1137336320 [M::process] read 728152 sequences (100000069 bp) in 3.563 CPU sec, 3.847 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 300029, 37, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 446, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1733) [M::mem_pestat] mean and std.dev: (450.27, 319.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 183, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (205.09, 99.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 361, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1931) [M::mem_pestat] mean and std.dev: (476.31, 407.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2520) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 396, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2585) [M::mem_pestat] mean and std.dev: (625.30, 580.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3363) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728688 reads in 229.577 CPU sec, 7.425 real sec [M::process] read 727878 sequences (100000009 bp) in 2.781 CPU sec, 2.854 real sec... [M::mem_process_seqs] Processed 728512 reads in 165.350 CPU sec, 5.472 real sec sorting block 143, #reads = 1095595 mem = 536877137 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 299525, 44, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 388, 1298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3290) [M::mem_pestat] mean and std.dev: (675.38, 602.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.60, 99.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 482, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3708) [M::mem_pestat] mean and std.dev: (624.16, 612.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 258, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368) [M::mem_pestat] mean and std.dev: (585.10, 597.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3085) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 728216 sequences (100000258 bp) in 3.107 CPU sec, 3.347 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 299500, 39, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 357, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358) [M::mem_pestat] mean and std.dev: (400.96, 261.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 184, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.64, 100.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 576, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3171) [M::mem_pestat] mean and std.dev: (720.24, 633.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4136) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 294, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029) [M::mem_pestat] mean and std.dev: (451.53, 399.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 727476 sequences (100000258 bp) in 2.471 CPU sec, 2.482 real sec... [M::mem_process_seqs] Processed 728152 reads in 233.826 CPU sec, 7.672 real sec sorting block 144, #reads = 1094934 mem = 536881441 peak = 1137336320 [M::mem_process_seqs] Processed 727878 reads in 154.661 CPU sec, 5.042 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 299324, 32, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 225, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (220.67, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (205.93, 99.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 442, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4560) [M::mem_pestat] mean and std.dev: (1021.97, 1159.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (371, 462, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2393) [M::mem_pestat] mean and std.dev: (601.95, 423.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 726678 sequences (100000099 bp) in 3.117 CPU sec, 3.278 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 299514, 33, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 313, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828) [M::mem_pestat] mean and std.dev: (462.05, 371.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 185, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.09, 100.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 722, 2458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7016) [M::mem_pestat] mean and std.dev: (1616.85, 1891.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 392, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1661) [M::mem_pestat] mean and std.dev: (412.49, 261.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 725978 sequences (100000209 bp) in 1.980 CPU sec, 1.972 real sec... [M::mem_process_seqs] Processed 728216 reads in 199.223 CPU sec, 6.455 real sec sorting block 145, #reads = 1093224 mem = 536878653 peak = 1137336320 [M::mem_process_seqs] Processed 727476 reads in 137.531 CPU sec, 4.394 real sec sorting block 146, #reads = 1095465 mem = 536878770 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 298662, 52, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 471, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4296) [M::mem_pestat] mean and std.dev: (907.38, 798.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 186, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (208.22, 101.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 532, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2532) [M::mem_pestat] mean and std.dev: (613.06, 560.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 624, 1362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3570) [M::mem_pestat] mean and std.dev: (808.77, 659.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724856 sequences (100000101 bp) in 2.679 CPU sec, 2.752 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 298588, 45, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 401, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3263) [M::mem_pestat] mean and std.dev: (668.98, 702.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 186, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (208.60, 101.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 629, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2683) [M::mem_pestat] mean and std.dev: (600.69, 510.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 289, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1529) [M::mem_pestat] mean and std.dev: (377.92, 298.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724936 sequences (100000220 bp) in 2.398 CPU sec, 2.401 real sec... [M::mem_process_seqs] Processed 726678 reads in 187.729 CPU sec, 5.933 real sec [M::mem_process_seqs] Processed 725978 reads in 133.975 CPU sec, 4.322 real sec sorting block 147, #reads = 1094473 mem = 536878463 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 297344, 40, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 339, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954) [M::mem_pestat] mean and std.dev: (469.97, 387.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.61, 102.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (227, 410, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4088) [M::mem_pestat] mean and std.dev: (913.10, 1047.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5375) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 435, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1920) [M::mem_pestat] mean and std.dev: (462.82, 345.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724618 sequences (100000183 bp) in 2.595 CPU sec, 2.661 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 297337, 47, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 588, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (519.16, 404.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.14, 101.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 806, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3067) [M::mem_pestat] mean and std.dev: (766.80, 635.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4019) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 307, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551) [M::mem_pestat] mean and std.dev: (339.67, 249.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724764 sequences (100000034 bp) in 2.170 CPU sec, 2.174 real sec... [M::mem_process_seqs] Processed 724856 reads in 177.142 CPU sec, 5.686 real sec sorting block 148, #reads = 1090284 mem = 536880193 peak = 1137336320 [M::mem_process_seqs] Processed 724936 reads in 120.254 CPU sec, 3.886 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 297635, 36, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 406, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2334) [M::mem_pestat] mean and std.dev: (555.55, 471.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.30, 101.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (353, 566, 1912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5030) [M::mem_pestat] mean and std.dev: (1088.97, 1253.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 454, 1309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3509) [M::mem_pestat] mean and std.dev: (682.83, 670.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724228 sequences (100000227 bp) in 2.696 CPU sec, 2.739 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 297358, 27, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 356, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1566) [M::mem_pestat] mean and std.dev: (408.21, 315.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (209.82, 100.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (426, 529, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1947) [M::mem_pestat] mean and std.dev: (660.19, 441.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2454) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 376, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2706) [M::mem_pestat] mean and std.dev: (580.04, 521.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724476 sequences (100000170 bp) in 2.230 CPU sec, 2.230 real sec... [M::mem_process_seqs] Processed 724618 reads in 177.209 CPU sec, 5.659 real sec sorting block 149, #reads = 1089985 mem = 536879666 peak = 1137336320 [M::mem_process_seqs] Processed 724764 reads in 122.916 CPU sec, 3.892 real sec sorting block 150, #reads = 1091211 mem = 536880175 peak = 1137336320 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 297918, 45, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 380, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2448) [M::mem_pestat] mean and std.dev: (593.83, 544.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.37, 101.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (255, 623, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3015) [M::mem_pestat] mean and std.dev: (756.61, 662.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 364, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2471) [M::mem_pestat] mean and std.dev: (587.92, 547.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724676 sequences (100000024 bp) in 2.385 CPU sec, 2.432 real sec... [M::process] read 723964 sequences (100000122 bp) in 1.919 CPU sec, 1.917 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 297964, 38, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 385, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2863) [M::mem_pestat] mean and std.dev: (592.70, 625.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (209.67, 101.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (428, 657, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3176) [M::mem_pestat] mean and std.dev: (645.03, 479.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 368, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254) [M::mem_pestat] mean and std.dev: (379.32, 233.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724228 reads in 186.503 CPU sec, 5.959 real sec [M::mem_process_seqs] Processed 724476 reads in 130.314 CPU sec, 4.273 real sec sorting block 151, #reads = 1091649 mem = 536879815 peak = 1137336320 [M::process] read 723836 sequences (100000088 bp) in 2.339 CPU sec, 2.398 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 297603, 34, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 383, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (573.26, 526.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (209.06, 100.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 623, 1808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4834) [M::mem_pestat] mean and std.dev: (755.69, 756.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (302, 804, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4007) [M::mem_pestat] mean and std.dev: (956.57, 791.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723780 sequences (100000293 bp) in 1.810 CPU sec, 1.812 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 297084, 46, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 231, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1470) [M::mem_pestat] mean and std.dev: (367.20, 314.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.58, 101.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (85, 617, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3520) [M::mem_pestat] mean and std.dev: (603.71, 584.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 428, 1962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5434) [M::mem_pestat] mean and std.dev: (872.41, 956.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 297275, 52, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 676, 1726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4726) [M::mem_pestat] mean and std.dev: (803.82, 800.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.06, 101.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (253, 603, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (645.21, 540.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 375, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1302) [M::mem_pestat] mean and std.dev: (431.56, 292.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1660) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724676 reads in 274.008 CPU sec, 8.869 real sec sorting block 152, #reads = 1090423 mem = 536878609 peak = 1137336320 [M::mem_process_seqs] Processed 723836 reads in 212.219 CPU sec, 6.808 real sec [M::mem_process_seqs] Processed 723964 reads in 244.746 CPU sec, 7.856 real sec sorting block 153, #reads = 1090615 mem = 536878339 peak = 1137336320 [M::process] read 724074 sequences (100000191 bp) in 2.332 CPU sec, 2.441 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 297083, 53, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 397, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1462) [M::mem_pestat] mean and std.dev: (453.67, 279.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.04, 101.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (180, 545, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3570) [M::mem_pestat] mean and std.dev: (647.87, 556.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 305, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1537) [M::mem_pestat] mean and std.dev: (355.06, 277.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724168 sequences (100000261 bp) in 2.443 CPU sec, 2.475 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 296622, 22, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 250, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3316) [M::mem_pestat] mean and std.dev: (556.46, 638.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (210.04, 101.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 667, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2162) [M::mem_pestat] mean and std.dev: (556.84, 411.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2796) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 422, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2021) [M::mem_pestat] mean and std.dev: (531.44, 418.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723780 reads in 178.290 CPU sec, 5.645 real sec [M::mem_process_seqs] Processed 724074 reads in 127.345 CPU sec, 3.997 real sec sorting block 154, #reads = 1085368 mem = 536879616 peak = 1137336320 [M::process] read 724398 sequences (100000134 bp) in 2.474 CPU sec, 2.497 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 297084, 76, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 262, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (392.21, 355.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.18, 101.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 539, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4376) [M::mem_pestat] mean and std.dev: (871.45, 964.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 539, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2754) [M::mem_pestat] mean and std.dev: (616.29, 492.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 725538 sequences (100000013 bp) in 2.772 CPU sec, 2.810 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 296807, 49, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 364, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2243) [M::mem_pestat] mean and std.dev: (508.74, 392.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (209.63, 101.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 636, 1567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4441) [M::mem_pestat] mean and std.dev: (806.33, 798.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 363, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2880) [M::mem_pestat] mean and std.dev: (577.04, 534.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 736970 sequences (100000017 bp) in 2.170 CPU sec, 2.176 real sec... [M::mem_process_seqs] Processed 724168 reads in 175.938 CPU sec, 5.557 real sec sorting block 155, #reads = 1088627 mem = 536880011 peak = 1137336320 [M::mem_process_seqs] Processed 724398 reads in 123.831 CPU sec, 3.902 real sec sorting block 156, #reads = 1087931 mem = 536880333 peak = 1177673728 [M::process] read 737708 sequences (100000294 bp) in 2.518 CPU sec, 2.591 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 296315, 39, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 386, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1968) [M::mem_pestat] mean and std.dev: (469.43, 397.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 186, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (207.30, 99.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 180, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172) [M::mem_pestat] mean and std.dev: (346.62, 422.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 290, 1440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3938) [M::mem_pestat] mean and std.dev: (819.52, 894.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 735576 sequences (100000015 bp) in 1.720 CPU sec, 1.717 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 301363, 49, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 421, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278) [M::mem_pestat] mean and std.dev: (517.57, 438.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (191.35, 88.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (302, 710, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4193) [M::mem_pestat] mean and std.dev: (791.46, 637.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5490) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 385, 905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2159) [M::mem_pestat] mean and std.dev: (531.69, 426.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725538 reads in 192.658 CPU sec, 6.110 real sec [M::mem_process_seqs] Processed 736970 reads in 218.709 CPU sec, 6.959 real sec sorting block 157, #reads = 1081462 mem = 536882805 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 301946, 40, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 405, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2568) [M::mem_pestat] mean and std.dev: (596.44, 569.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (190.34, 87.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 627, 1372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3674) [M::mem_pestat] mean and std.dev: (866.16, 839.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 409, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2742) [M::mem_pestat] mean and std.dev: (639.91, 571.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 734662 sequences (100000015 bp) in 2.907 CPU sec, 2.949 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 302061, 24, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 262, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3331) [M::mem_pestat] mean and std.dev: (645.05, 759.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (194.16, 90.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 380, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2428) [M::mem_pestat] mean and std.dev: (566.92, 456.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 410, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2470) [M::mem_pestat] mean and std.dev: (628.33, 531.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 733702 sequences (100000151 bp) in 1.970 CPU sec, 1.963 real sec... [M::mem_process_seqs] Processed 737708 reads in 173.206 CPU sec, 5.475 real sec sorting block 158, #reads = 1090898 mem = 536880194 peak = 1177673728 [M::mem_process_seqs] Processed 735576 reads in 200.417 CPU sec, 6.364 real sec sorting block 159, #reads = 1095612 mem = 536879324 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 301385, 36, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 403, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3011) [M::mem_pestat] mean and std.dev: (715.38, 767.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 732540 sequences (100000050 bp) in 2.232 CPU sec, 2.281 real sec... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (194.84, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 628, 1858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5124) [M::mem_pestat] mean and std.dev: (829.00, 901.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6757) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 388, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2261) [M::mem_pestat] mean and std.dev: (537.84, 451.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 734474 sequences (100000246 bp) in 1.720 CPU sec, 1.721 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 301521, 44, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 550, 1881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5201) [M::mem_pestat] mean and std.dev: (958.31, 1092.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 177, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (196.57, 92.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 459, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2350) [M::mem_pestat] mean and std.dev: (554.31, 472.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 386, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016) [M::mem_pestat] mean and std.dev: (491.59, 412.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2632) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 734662 reads in 170.389 CPU sec, 5.399 real sec [M::mem_process_seqs] Processed 733702 reads in 115.565 CPU sec, 3.680 real sec sorting block 160, #reads = 1096140 mem = 536883909 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 301109, 40, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 352, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2476) [M::mem_pestat] mean and std.dev: (443.92, 400.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 178, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (197.96, 93.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 698, 1663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4753) [M::mem_pestat] mean and std.dev: (852.50, 848.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 339, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1991) [M::mem_pestat] mean and std.dev: (431.38, 353.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 734576 sequences (100000133 bp) in 2.853 CPU sec, 2.963 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 301810, 35, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 292, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (303.20, 230.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 177, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (195.55, 91.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 258, 1111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3079) [M::mem_pestat] mean and std.dev: (588.44, 692.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 461, 1314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3552) [M::mem_pestat] mean and std.dev: (706.24, 628.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 732804 sequences (100000215 bp) in 1.960 CPU sec, 1.957 real sec... [M::mem_process_seqs] Processed 732540 reads in 166.994 CPU sec, 5.276 real sec [M::mem_process_seqs] Processed 734474 reads in 116.986 CPU sec, 3.698 real sec sorting block 161, #reads = 1096512 mem = 536880082 peak = 1177673728 [M::process] read 731442 sequences (100000033 bp) in 2.372 CPU sec, 2.418 real sec... [M::process] read 732914 sequences (100000088 bp) in 1.740 CPU sec, 1.738 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 300333, 45, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 437, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3723) [M::mem_pestat] mean and std.dev: (678.10, 585.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 179, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (199.74, 94.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (290, 407, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (423.57, 261.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 435, 1705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4571) [M::mem_pestat] mean and std.dev: (799.87, 712.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 731442 reads in 91.198 CPU sec, 2.891 real sec sorting block 162, #reads = 1094716 mem = 536887102 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 301442, 28, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 346, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1999) [M::mem_pestat] mean and std.dev: (456.33, 410.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (194.55, 90.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (116, 773, 2862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8354) [M::mem_pestat] mean and std.dev: (1471.61, 1695.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11100) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 531, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2859) [M::mem_pestat] mean and std.dev: (730.63, 606.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 301115, 25, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 855, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3395) [M::mem_pestat] mean and std.dev: (816.15, 680.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (197.29, 93.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 610, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4234) [M::mem_pestat] mean and std.dev: (965.17, 763.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 318, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1756) [M::mem_pestat] mean and std.dev: (486.03, 388.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2283) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 730236 sequences (100000252 bp) in 2.143 CPU sec, 2.150 real sec... [M::mem_process_seqs] Processed 734576 reads in 266.902 CPU sec, 8.444 real sec sorting block 163, #reads = 1092061 mem = 536878804 peak = 1177673728 [M::mem_process_seqs] Processed 732804 reads in 207.044 CPU sec, 6.572 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 300080, 55, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 392, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2531) [M::mem_pestat] mean and std.dev: (612.85, 482.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (201.13, 95.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (402, 629, 1449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3543) [M::mem_pestat] mean and std.dev: (776.38, 660.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 387, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876) [M::mem_pestat] mean and std.dev: (469.86, 322.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2422) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 730422 sequences (100000292 bp) in 2.438 CPU sec, 2.481 real sec... [M::process] read 730308 sequences (100000103 bp) in 1.710 CPU sec, 1.706 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 301141, 31, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 349, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2915) [M::mem_pestat] mean and std.dev: (569.67, 527.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 177, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (195.31, 90.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (155, 360, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2870) [M::mem_pestat] mean and std.dev: (556.46, 527.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 228, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215) [M::mem_pestat] mean and std.dev: (278.47, 250.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 730236 reads in 159.728 CPU sec, 5.064 real sec sorting block 164, #reads = 1094797 mem = 536885479 peak = 1177673728 [M::mem_process_seqs] Processed 732914 reads in 181.351 CPU sec, 5.768 real sec sorting block 165, #reads = 1093558 mem = 536878443 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 300592, 52, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 381, 854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2160) [M::mem_pestat] mean and std.dev: (541.84, 450.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (201.12, 96.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 571, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3830) [M::mem_pestat] mean and std.dev: (706.57, 682.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 360, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617) [M::mem_pestat] mean and std.dev: (611.51, 554.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 728624 sequences (100000150 bp) in 2.194 CPU sec, 2.240 real sec... [M::process] read 727928 sequences (100000172 bp) in 1.710 CPU sec, 1.709 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 299969, 38, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 261, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2930) [M::mem_pestat] mean and std.dev: (660.51, 781.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (201.45, 96.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 873, 1799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4917) [M::mem_pestat] mean and std.dev: (1197.19, 1202.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 380, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2231) [M::mem_pestat] mean and std.dev: (566.62, 439.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2892) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 730422 reads in 158.118 CPU sec, 5.006 real sec [M::mem_process_seqs] Processed 730308 reads in 108.678 CPU sec, 3.457 real sec sorting block 166, #reads = 1094624 mem = 536878641 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 299245, 45, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 784, 2538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6808) [M::mem_pestat] mean and std.dev: (1290.03, 1327.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 183, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (203.93, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 593, 1383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3669) [M::mem_pestat] mean and std.dev: (671.18, 627.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 367, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2198) [M::mem_pestat] mean and std.dev: (486.24, 345.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 727250 sequences (100000180 bp) in 2.469 CPU sec, 2.505 real sec... [M::process] read 725954 sequences (100000100 bp) in 1.670 CPU sec, 1.670 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 298747, 42, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 330, 804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1982) [M::mem_pestat] mean and std.dev: (474.94, 377.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 184, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (205.10, 98.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (197, 760, 1988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5570) [M::mem_pestat] mean and std.dev: (832.22, 805.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 322, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2276) [M::mem_pestat] mean and std.dev: (430.14, 360.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728624 reads in 154.810 CPU sec, 4.892 real sec [M::mem_process_seqs] Processed 727928 reads in 174.436 CPU sec, 5.533 real sec sorting block 167, #reads = 1092690 mem = 536882915 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 298426, 42, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 373, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1896) [M::mem_pestat] mean and std.dev: (416.79, 333.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 185, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (205.59, 98.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 596, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301) [M::mem_pestat] mean and std.dev: (763.50, 739.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 315, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767) [M::mem_pestat] mean and std.dev: (436.37, 359.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2291) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 725684 sequences (100000255 bp) in 2.248 CPU sec, 2.311 real sec... [M::process] read 725536 sequences (100000257 bp) in 1.660 CPU sec, 1.655 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 297703, 51, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 573, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2443) [M::mem_pestat] mean and std.dev: (655.36, 489.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 186, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (207.34, 99.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (308, 842, 2286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6242) [M::mem_pestat] mean and std.dev: (1167.12, 1127.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 492, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1881) [M::mem_pestat] mean and std.dev: (514.29, 363.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727250 reads in 157.604 CPU sec, 4.994 real sec sorting block 168, #reads = 1092831 mem = 536880740 peak = 1177673728 [M::mem_process_seqs] Processed 725954 reads in 111.086 CPU sec, 3.526 real sec sorting block 169, #reads = 1088786 mem = 536882556 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 298077, 29, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 310, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1773) [M::mem_pestat] mean and std.dev: (468.96, 416.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (208.46, 100.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 500, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4798) [M::mem_pestat] mean and std.dev: (1075.46, 1132.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 444, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3194) [M::mem_pestat] mean and std.dev: (756.52, 685.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 725092 sequences (100000195 bp) in 2.215 CPU sec, 2.264 real sec... [M::process] read 724972 sequences (100000148 bp) in 1.740 CPU sec, 1.742 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 298206, 49, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 360, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2862) [M::mem_pestat] mean and std.dev: (656.30, 624.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 186, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (207.38, 99.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (253, 498, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2752) [M::mem_pestat] mean and std.dev: (623.26, 496.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 352, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3720) [M::mem_pestat] mean and std.dev: (770.48, 877.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725684 reads in 153.959 CPU sec, 4.870 real sec [M::mem_process_seqs] Processed 725536 reads in 103.953 CPU sec, 3.292 real sec sorting block 170, #reads = 1091495 mem = 536877511 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 297757, 43, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 758, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2972) [M::mem_pestat] mean and std.dev: (723.78, 611.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (208.50, 100.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (337, 633, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3595) [M::mem_pestat] mean and std.dev: (900.38, 847.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412) [M::mem_pestat] mean and std.dev: (370.52, 282.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 724698 sequences (100000073 bp) in 2.248 CPU sec, 2.297 real sec... [M::process] read 723268 sequences (100000264 bp) in 1.640 CPU sec, 1.640 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 297164, 28, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 432, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3419) [M::mem_pestat] mean and std.dev: (743.53, 733.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (208.04, 99.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (136, 289, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2518) [M::mem_pestat] mean and std.dev: (454.04, 495.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 475, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4147) [M::mem_pestat] mean and std.dev: (1004.10, 1090.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725092 reads in 150.670 CPU sec, 4.769 real sec sorting block 171, #reads = 1091212 mem = 536876452 peak = 1177673728 [M::mem_process_seqs] Processed 724972 reads in 102.434 CPU sec, 3.243 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 297505, 42, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 644, 1855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5123) [M::mem_pestat] mean and std.dev: (831.03, 795.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (209.62, 100.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (430, 975, 1836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4648) [M::mem_pestat] mean and std.dev: (1091.88, 766.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 354, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2238) [M::mem_pestat] mean and std.dev: (482.07, 449.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723188 sequences (100000227 bp) in 2.300 CPU sec, 2.324 real sec... [M::process] read 723248 sequences (100000093 bp) in 1.590 CPU sec, 1.596 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 296877, 53, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 339, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2849) [M::mem_pestat] mean and std.dev: (433.54, 400.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.24, 101.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (116, 792, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4001) [M::mem_pestat] mean and std.dev: (818.40, 828.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 284, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2349) [M::mem_pestat] mean and std.dev: (537.51, 513.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724698 reads in 145.708 CPU sec, 4.608 real sec sorting block 172, #reads = 1092540 mem = 536878692 peak = 1177673728 [M::mem_process_seqs] Processed 723268 reads in 99.907 CPU sec, 3.171 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 296942, 45, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 425, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2055) [M::mem_pestat] mean and std.dev: (443.79, 341.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.43, 102.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (308, 770, 1249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3131) [M::mem_pestat] mean and std.dev: (697.64, 560.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 281, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4182) [M::mem_pestat] mean and std.dev: (763.80, 989.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5504) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 173, #reads = 1090904 mem = 536878185 peak = 1177673728 [M::process] read 722906 sequences (100000052 bp) in 3.325 CPU sec, 3.448 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 297290, 25, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 341, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2148) [M::mem_pestat] mean and std.dev: (466.23, 451.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (210.91, 101.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (166, 373, 1329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3655) [M::mem_pestat] mean and std.dev: (733.12, 792.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4818) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 360, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2764) [M::mem_pestat] mean and std.dev: (686.39, 701.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723248 reads in 155.035 CPU sec, 4.899 real sec [M::mem_process_seqs] Processed 723188 reads in 174.525 CPU sec, 5.522 real sec [M::process] read 722826 sequences (100000157 bp) in 2.265 CPU sec, 2.320 real sec... sorting block 174, #reads = 1091391 mem = 536880841 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 296792, 36, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 382, 1020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2736) [M::mem_pestat] mean and std.dev: (562.90, 611.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.76, 102.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (476, 949, 2904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7760) [M::mem_pestat] mean and std.dev: (1699.61, 1758.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10188) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 334, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1664) [M::mem_pestat] mean and std.dev: (448.29, 359.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723186 sequences (100000254 bp) in 2.634 CPU sec, 2.751 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 296988, 50, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 255, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2065) [M::mem_pestat] mean and std.dev: (420.57, 377.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.47, 102.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (346, 661, 1471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3721) [M::mem_pestat] mean and std.dev: (867.06, 734.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 534, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (557.74, 390.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 722906 reads in 148.526 CPU sec, 4.711 real sec [M::mem_process_seqs] Processed 722826 reads in 101.503 CPU sec, 3.192 real sec [M::process] read 723336 sequences (100000179 bp) in 2.101 CPU sec, 2.135 real sec... sorting block 175, #reads = 1090344 mem = 536878332 peak = 1177673728 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 297220, 45, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 558, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4458) [M::mem_pestat] mean and std.dev: (777.25, 791.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (212.04, 102.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 416, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3285) [M::mem_pestat] mean and std.dev: (603.67, 631.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 341, 1796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4884) [M::mem_pestat] mean and std.dev: (801.97, 986.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 723924 sequences (100000199 bp) in 2.524 CPU sec, 2.592 real sec... [M::process] read 19176 sequences (2617730 bp) in 0.250 CPU sec, 0.248 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 296987, 48, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 628, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2868) [M::mem_pestat] mean and std.dev: (748.53, 605.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.25, 101.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 299, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2230) [M::mem_pestat] mean and std.dev: (528.75, 522.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 294, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729) [M::mem_pestat] mean and std.dev: (462.86, 423.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723186 reads in 145.015 CPU sec, 4.649 real sec sorting block 176, #reads = 1091696 mem = 536880934 peak = 1177673728 [M::mem_process_seqs] Processed 723336 reads in 99.303 CPU sec, 3.166 real sec sorting block 177, #reads = 1091397 mem = 536878460 peak = 1236750336 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 297164, 51, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 323, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3987) [M::mem_pestat] mean and std.dev: (873.90, 926.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.36, 101.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (366, 747, 1713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4407) [M::mem_pestat] mean and std.dev: (901.59, 783.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 443, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2976) [M::mem_pestat] mean and std.dev: (743.59, 686.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 7747, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 183, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (204.52, 99.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 723924 reads in 87.949 CPU sec, 2.854 real sec [M::mem_process_seqs] Processed 19176 reads in 21.847 CPU sec, 0.703 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 193850810 overall: 70743777280 mem 32894.255 user 672.930 sys 1119.942 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_4Z5-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1136_4Z5-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_4Z5-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_4Z5-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_4Z5-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 1119.987 sec; CPU: 33567.192 sec sorting block 178, #reads = 959394 mem = 473395725 peak = 1236750336 uniq header count 1 merging 179 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 9976142761 output reads: 195628829 bam_mem_sort: 179 calls 121.032 user 3.156 sys 126.719 real bam_write: 179 calls 116.990 user 7.487 sys 218.410 real execute: 1 calls 41.161 user 1.639 sys 1179.071 real merge_files: 1 calls 41.141 user 1.598 sys 57.791 real parse_chunk: 2863 calls 170.865 user 42.712 sys 252.527 real read_chunk: 7762 calls 87.604 user 51.567 sys 1067.362 real sort_block: 1 calls 71.637 user 51.183 sys 1043.310 real write_chunk: 4897 calls 40.822 user 1.258 sys 56.855 real overall: 4129386496 mem 2503.035 user 131.370 sys 1179.095 real ls: cannot access '*.cram': No such file or directory