Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/69/ea21ff7199417a10bacb1db911320d/1136_4GR-T1-TDNA-1_A23MHWWLT4_1/1136_4GR-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ff/e5d8f31fa5d9aac14b55f6de8f3b53/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ff/e5d8f31fa5d9aac14b55f6de8f3b53/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/69/ea21ff7199417a10bacb1db911320d/1136_4GR-T1-TDNA-1_A23MHWWLT4_1/1136_4GR-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz ==> STAGING COMPLETE (7 inputs) This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_4GR-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2615.191 CPU sec, 108.997 real sec [M::process] read 701216 sequences (100000019 bp) in 1.401 CPU sec, 1.407 real sec... [M::process] read 699474 sequences (100000079 bp) in 2.109 CPU sec, 2.128 real sec... [M::process] read 698764 sequences (100000094 bp) in 1.824 CPU sec, 1.842 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285246, 31, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 305, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (263.69, 112.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (258.14, 129.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 263, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392) [M::mem_pestat] mean and std.dev: (646.03, 951.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 331, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337) [M::mem_pestat] mean and std.dev: (307.38, 158.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698194 sequences (100000249 bp) in 1.594 CPU sec, 1.628 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 284078, 16, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 320, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346) [M::mem_pestat] mean and std.dev: (334.05, 243.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (266.24, 134.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 958, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3575) [M::mem_pestat] mean and std.dev: (722.53, 593.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4718) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 326, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771) [M::mem_pestat] mean and std.dev: (332.89, 200.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284427, 34, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 425, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852) [M::mem_pestat] mean and std.dev: (441.93, 292.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (263.46, 133.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (904, 1512, 2702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6298) [M::mem_pestat] mean and std.dev: (1369.41, 866.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8096) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 320, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2634) [M::mem_pestat] mean and std.dev: (650.32, 652.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701216 reads in 330.194 CPU sec, 10.626 real sec [M::mem_process_seqs] Processed 698764 reads in 223.686 CPU sec, 7.278 real sec [M::mem_process_seqs] Processed 699474 reads in 247.121 CPU sec, 8.020 real sec sorting block 0, #reads = 1078143 mem = 536878647 peak = 568868864 [M::process] read 697952 sequences (100000183 bp) in 3.340 CPU sec, 3.680 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283627, 29, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 271, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1501) [M::mem_pestat] mean and std.dev: (356.15, 290.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (269.13, 136.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (623, 776, 1520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3314) [M::mem_pestat] mean and std.dev: (1013.04, 730.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 674, 2577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7331) [M::mem_pestat] mean and std.dev: (1672.77, 2093.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697234 sequences (100000225 bp) in 2.265 CPU sec, 2.374 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283871, 19, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 246, 1964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5578) [M::mem_pestat] mean and std.dev: (905.66, 1164.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (270.25, 137.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 486, 3776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10926) [M::mem_pestat] mean and std.dev: (2484.74, 3100.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 533, 1591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4279) [M::mem_pestat] mean and std.dev: (875.42, 874.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698194 reads in 200.401 CPU sec, 6.383 real sec sorting block 1, #reads = 1077277 mem = 536881092 peak = 752381952 [M::mem_process_seqs] Processed 697952 reads in 131.622 CPU sec, 4.154 real sec [M::process] read 697118 sequences (100000056 bp) in 2.050 CPU sec, 2.137 real sec... sorting block 2, #reads = 1077295 mem = 536879813 peak = 752381952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283982, 23, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 382, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1520) [M::mem_pestat] mean and std.dev: (415.92, 262.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (272.54, 138.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (360, 1008, 1573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3999) [M::mem_pestat] mean and std.dev: (1047.10, 978.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 228, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (313.42, 240.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696362 sequences (100000099 bp) in 2.752 CPU sec, 2.975 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283649, 37, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 359, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3249) [M::mem_pestat] mean and std.dev: (357.13, 309.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (273.04, 139.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 476, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3581) [M::mem_pestat] mean and std.dev: (795.36, 807.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4722) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 337, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (497.59, 392.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696138 sequences (100000300 bp) in 2.003 CPU sec, 2.011 real sec... [M::mem_process_seqs] Processed 697234 reads in 209.033 CPU sec, 6.666 real sec [M::mem_process_seqs] Processed 697118 reads in 133.579 CPU sec, 4.245 real sec sorting block 3, #reads = 1077046 mem = 536882683 peak = 752381952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283851, 24, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 380, 2003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5493) [M::mem_pestat] mean and std.dev: (1088.09, 1510.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (275.02, 139.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (481, 697, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1951) [M::mem_pestat] mean and std.dev: (654.52, 386.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2441) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 350, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (442.91, 438.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695746 sequences (100000222 bp) in 3.498 CPU sec, 3.730 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 283394, 29, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 510, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2853) [M::mem_pestat] mean and std.dev: (598.98, 517.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.83, 140.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (154, 1049, 2260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6472) [M::mem_pestat] mean and std.dev: (1176.96, 1203.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 344, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (344.62, 228.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696362 reads in 197.027 CPU sec, 6.288 real sec [M::process] read 695842 sequences (100000206 bp) in 1.800 CPU sec, 1.800 real sec... [M::mem_process_seqs] Processed 696138 reads in 132.648 CPU sec, 4.551 real sec sorting block 4, #reads = 1077134 mem = 536878852 peak = 752381952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283319, 34, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 304, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207) [M::mem_pestat] mean and std.dev: (300.00, 207.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (275.68, 140.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (354, 706, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925) [M::mem_pestat] mean and std.dev: (625.04, 411.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3782) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 317, 1283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3389) [M::mem_pestat] mean and std.dev: (706.48, 599.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4442) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696188 sequences (100000021 bp) in 2.730 CPU sec, 2.943 real sec... [M::process] read 696462 sequences (100000254 bp) in 1.837 CPU sec, 1.871 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283025, 32, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 303, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3130) [M::mem_pestat] mean and std.dev: (551.82, 642.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (275.79, 140.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 912, 2106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6004) [M::mem_pestat] mean and std.dev: (1380.50, 1244.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 206, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146) [M::mem_pestat] mean and std.dev: (304.71, 236.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695746 reads in 214.375 CPU sec, 7.198 real sec sorting block 5, #reads = 1076860 mem = 536879207 peak = 752381952 [M::mem_process_seqs] Processed 695842 reads in 137.574 CPU sec, 4.401 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282931, 28, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (350, 720, 2480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6740) [M::mem_pestat] mean and std.dev: (1481.12, 1585.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (274.55, 139.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 294, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855) [M::mem_pestat] mean and std.dev: (411.89, 370.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (543, 725, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3018) [M::mem_pestat] mean and std.dev: (669.38, 358.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 6, #reads = 1074967 mem = 536877914 peak = 807546880 [M::process] read 696364 sequences (100000197 bp) in 3.214 CPU sec, 3.503 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283121, 23, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 360, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (498.88, 428.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.81, 138.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 349, 1249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3219) [M::mem_pestat] mean and std.dev: (838.13, 844.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 368, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2474) [M::mem_pestat] mean and std.dev: (499.68, 415.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696480 sequences (100000293 bp) in 1.969 CPU sec, 1.967 real sec... [M::mem_process_seqs] Processed 696188 reads in 207.245 CPU sec, 6.625 real sec [M::mem_process_seqs] Processed 696462 reads in 146.960 CPU sec, 4.704 real sec sorting block 7, #reads = 1075950 mem = 536880273 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 284003, 27, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1491) [M::mem_pestat] mean and std.dev: (412.40, 314.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.22, 137.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 423, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4004) [M::mem_pestat] mean and std.dev: (654.71, 743.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 427, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2853) [M::mem_pestat] mean and std.dev: (609.57, 516.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695966 sequences (100000089 bp) in 3.942 CPU sec, 4.302 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283580, 26, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 259, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2322) [M::mem_pestat] mean and std.dev: (563.05, 538.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3035) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 244, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.60, 138.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 637, 3137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9003) [M::mem_pestat] mean and std.dev: (1344.52, 1498.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 364, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2133) [M::mem_pestat] mean and std.dev: (488.21, 400.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696364 reads in 206.182 CPU sec, 6.592 real sec [M::mem_process_seqs] Processed 696480 reads in 135.245 CPU sec, 4.279 real sec [M::process] read 695838 sequences (100000123 bp) in 3.542 CPU sec, 3.788 real sec... sorting block 8, #reads = 1075870 mem = 536880244 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 283291, 18, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 313, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3225) [M::mem_pestat] mean and std.dev: (643.35, 706.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (273.40, 138.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 397, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1919) [M::mem_pestat] mean and std.dev: (437.12, 351.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 393, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3475) [M::mem_pestat] mean and std.dev: (831.44, 713.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695708 sequences (100000066 bp) in 3.348 CPU sec, 3.634 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283098, 31, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 569, 1573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4259) [M::mem_pestat] mean and std.dev: (980.61, 881.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (274.09, 138.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 591, 1704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4874) [M::mem_pestat] mean and std.dev: (925.87, 972.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 380, 1252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3334) [M::mem_pestat] mean and std.dev: (654.30, 561.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695966 reads in 207.788 CPU sec, 6.676 real sec [M::mem_process_seqs] Processed 695838 reads in 136.995 CPU sec, 4.348 real sec sorting block 9, #reads = 1075857 mem = 536879217 peak = 807546880 [M::process] read 694378 sequences (100000068 bp) in 2.662 CPU sec, 2.802 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283609, 19, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 755, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4064) [M::mem_pestat] mean and std.dev: (879.65, 785.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (274.30, 138.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (142, 524, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4981) [M::mem_pestat] mean and std.dev: (977.84, 1097.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 368, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527) [M::mem_pestat] mean and std.dev: (522.25, 487.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 10, #reads = 1076638 mem = 536876921 peak = 807546880 [M::process] read 695064 sequences (100000143 bp) in 3.051 CPU sec, 3.297 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282459, 22, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 393, 1416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3898) [M::mem_pestat] mean and std.dev: (700.29, 738.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (278.56, 139.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 350, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1722) [M::mem_pestat] mean and std.dev: (358.60, 301.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 360, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5222) [M::mem_pestat] mean and std.dev: (1090.89, 1301.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695708 reads in 199.227 CPU sec, 6.403 real sec [M::mem_process_seqs] Processed 694378 reads in 132.941 CPU sec, 4.227 real sec [M::process] read 694498 sequences (100000179 bp) in 2.762 CPU sec, 2.925 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282810, 38, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 405, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (432.15, 277.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.21, 138.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 419, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5425) [M::mem_pestat] mean and std.dev: (1033.32, 1093.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 455, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1641) [M::mem_pestat] mean and std.dev: (519.02, 381.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 11, #reads = 1077466 mem = 536877974 peak = 807546880 [M::process] read 694696 sequences (100000144 bp) in 3.178 CPU sec, 3.443 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282824, 35, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (439, 677, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2470) [M::mem_pestat] mean and std.dev: (727.74, 510.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (276.60, 138.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (232, 568, 1619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4393) [M::mem_pestat] mean and std.dev: (1004.76, 1088.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5780) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 375, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3059) [M::mem_pestat] mean and std.dev: (577.06, 508.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695064 reads in 203.298 CPU sec, 6.521 real sec [M::process] read 694556 sequences (100000256 bp) in 2.151 CPU sec, 2.220 real sec... [M::mem_process_seqs] Processed 694498 reads in 136.104 CPU sec, 4.424 real sec sorting block 12, #reads = 1075745 mem = 536879533 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282612, 17, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 290, 1610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4434) [M::mem_pestat] mean and std.dev: (752.18, 900.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (275.72, 136.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (556, 971, 2220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5548) [M::mem_pestat] mean and std.dev: (1155.41, 847.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 270, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505) [M::mem_pestat] mean and std.dev: (502.22, 461.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694390 sequences (100000280 bp) in 3.008 CPU sec, 3.405 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282122, 30, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 408, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2078) [M::mem_pestat] mean and std.dev: (618.03, 543.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (278.30, 139.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (341, 715, 1500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3818) [M::mem_pestat] mean and std.dev: (866.04, 822.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4977) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 317, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (332.43, 182.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694696 reads in 205.885 CPU sec, 6.705 real sec [M::mem_process_seqs] Processed 694556 reads in 137.612 CPU sec, 4.375 real sec [M::process] read 694018 sequences (100000138 bp) in 2.819 CPU sec, 2.914 real sec... sorting block 13, #reads = 1075804 mem = 536881669 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 282741, 19, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 428, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4104) [M::mem_pestat] mean and std.dev: (872.56, 979.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (279.43, 140.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 719, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2339) [M::mem_pestat] mean and std.dev: (745.94, 431.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 288, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (332.64, 183.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696108 sequences (100000087 bp) in 2.892 CPU sec, 3.073 real sec... sorting block 14, #reads = 1076529 mem = 536880465 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 282829, 15, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 336, 1706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4662) [M::mem_pestat] mean and std.dev: (734.92, 764.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (282.20, 142.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (494, 591, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1997) [M::mem_pestat] mean and std.dev: (563.25, 345.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 241, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (304.95, 237.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694390 reads in 198.201 CPU sec, 6.354 real sec [M::process] read 699728 sequences (100000147 bp) in 2.131 CPU sec, 2.171 real sec... [M::mem_process_seqs] Processed 694018 reads in 134.731 CPU sec, 4.268 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 283040, 23, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 389, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2491) [M::mem_pestat] mean and std.dev: (563.49, 524.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (274.05, 137.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 636, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2161) [M::mem_pestat] mean and std.dev: (561.00, 373.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 664, 1607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4253) [M::mem_pestat] mean and std.dev: (876.57, 756.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5576) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 15, #reads = 1075399 mem = 536879034 peak = 807546880 [M::process] read 699262 sequences (100000280 bp) in 3.252 CPU sec, 3.483 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284378, 13, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 341, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (329.31, 147.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (263.38, 133.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (553, 832, 2161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5377) [M::mem_pestat] mean and std.dev: (1283.38, 1119.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6985) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 339, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1833) [M::mem_pestat] mean and std.dev: (445.35, 391.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2379) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698552 sequences (100000181 bp) in 1.751 CPU sec, 1.756 real sec... [M::mem_process_seqs] Processed 696108 reads in 205.247 CPU sec, 6.559 real sec [M::mem_process_seqs] Processed 699728 reads in 135.991 CPU sec, 4.360 real sec sorting block 16, #reads = 1074903 mem = 536878317 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284504, 28, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 390, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (320.89, 141.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (267.33, 136.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 359, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2301) [M::mem_pestat] mean and std.dev: (664.74, 675.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 298, 879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2255) [M::mem_pestat] mean and std.dev: (543.34, 563.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698242 sequences (100000111 bp) in 3.708 CPU sec, 4.009 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283723, 14, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 335, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2602) [M::mem_pestat] mean and std.dev: (574.61, 643.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (269.51, 138.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 778, 1586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4586) [M::mem_pestat] mean and std.dev: (987.64, 920.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3787) [M::mem_pestat] mean and std.dev: (610.33, 741.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699262 reads in 198.520 CPU sec, 6.385 real sec [M::mem_process_seqs] Processed 698552 reads in 131.719 CPU sec, 4.166 real sec [M::process] read 697512 sequences (100000038 bp) in 2.459 CPU sec, 2.567 real sec... sorting block 17, #reads = 1080573 mem = 536877608 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283897, 28, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 495, 1146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020) [M::mem_pestat] mean and std.dev: (649.10, 621.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (271.00, 139.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 1527, 1850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5068) [M::mem_pestat] mean and std.dev: (1306.78, 1214.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6677) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 701, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4779) [M::mem_pestat] mean and std.dev: (610.70, 529.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697238 sequences (100000145 bp) in 3.167 CPU sec, 3.441 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284055, 14, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 366, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (359.26, 208.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (273.54, 140.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (75, 705, 3169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9357) [M::mem_pestat] mean and std.dev: (1426.00, 1463.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 413, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4372) [M::mem_pestat] mean and std.dev: (831.42, 947.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698242 reads in 198.594 CPU sec, 6.362 real sec sorting block 18, #reads = 1079228 mem = 536878069 peak = 807546880 [M::mem_process_seqs] Processed 697512 reads in 133.128 CPU sec, 4.210 real sec [M::process] read 696606 sequences (100000251 bp) in 2.577 CPU sec, 2.699 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283916, 17, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 382, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2318) [M::mem_pestat] mean and std.dev: (514.42, 395.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (274.09, 140.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (455, 579, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2567) [M::mem_pestat] mean and std.dev: (760.41, 647.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 438, 2370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6580) [M::mem_pestat] mean and std.dev: (1206.64, 1116.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8685) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 19, #reads = 1079072 mem = 536883147 peak = 807546880 [M::process] read 696422 sequences (100000046 bp) in 3.139 CPU sec, 3.343 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283756, 21, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 885, 2148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5802) [M::mem_pestat] mean and std.dev: (1085.16, 867.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.57, 141.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (231, 524, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3102) [M::mem_pestat] mean and std.dev: (693.95, 541.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4059) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 374, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2960) [M::mem_pestat] mean and std.dev: (553.37, 495.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697238 reads in 199.682 CPU sec, 6.396 real sec [M::mem_process_seqs] Processed 696606 reads in 132.253 CPU sec, 4.183 real sec [M::process] read 696402 sequences (100000297 bp) in 2.501 CPU sec, 2.608 real sec... sorting block 20, #reads = 1078127 mem = 536879237 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283481, 12, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 422, 1630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4532) [M::mem_pestat] mean and std.dev: (911.12, 1136.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (276.76, 142.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (406, 845, 1635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4093) [M::mem_pestat] mean and std.dev: (1113.50, 912.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 427, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2094) [M::mem_pestat] mean and std.dev: (443.11, 333.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695728 sequences (100000113 bp) in 3.579 CPU sec, 3.931 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283547, 32, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 407, 2229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6257) [M::mem_pestat] mean and std.dev: (1030.38, 1294.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (277.31, 142.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (132, 884, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3375) [M::mem_pestat] mean and std.dev: (764.23, 737.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 644, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2998) [M::mem_pestat] mean and std.dev: (646.20, 527.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696422 reads in 194.768 CPU sec, 6.249 real sec [M::mem_process_seqs] Processed 696402 reads in 128.976 CPU sec, 4.088 real sec sorting block 21, #reads = 1077759 mem = 536880383 peak = 807546880 [M::process] read 695656 sequences (100000191 bp) in 3.674 CPU sec, 4.011 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283407, 33, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 334, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424) [M::mem_pestat] mean and std.dev: (373.33, 249.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (277.96, 142.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (569, 1009, 1573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3581) [M::mem_pestat] mean and std.dev: (1076.15, 746.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 369, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (498.47, 374.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283330, 19, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 269, 1757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4821) [M::mem_pestat] mean and std.dev: (790.53, 1042.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (277.64, 142.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 454, 1333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3615) [M::mem_pestat] mean and std.dev: (689.17, 595.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 326, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (307.74, 113.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695728 reads in 192.380 CPU sec, 6.258 real sec [M::mem_process_seqs] Processed 695656 reads in 128.045 CPU sec, 4.116 real sec [M::process] read 696238 sequences (100000015 bp) in 3.741 CPU sec, 4.156 real sec... sorting block 22, #reads = 1076917 mem = 536888708 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283311, 25, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 272, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461) [M::mem_pestat] mean and std.dev: (384.45, 368.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (275.06, 140.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (327, 912, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2751) [M::mem_pestat] mean and std.dev: (685.90, 568.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3559) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 330, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2132) [M::mem_pestat] mean and std.dev: (541.23, 495.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696562 sequences (100000162 bp) in 3.063 CPU sec, 3.410 real sec... [M::mem_process_seqs] Processed 696238 reads in 106.982 CPU sec, 3.983 real sec sorting block 23, #reads = 1076159 mem = 536878247 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283017, 25, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 424, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718) [M::mem_pestat] mean and std.dev: (472.79, 336.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (273.21, 139.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 1693, 2194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5968) [M::mem_pestat] mean and std.dev: (1222.00, 882.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 271, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1936) [M::mem_pestat] mean and std.dev: (380.44, 315.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696406 sequences (100000218 bp) in 3.811 CPU sec, 4.137 real sec... [M::mem_process_seqs] Processed 696562 reads in 111.542 CPU sec, 4.077 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 282950, 16, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 666, 1565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4205) [M::mem_pestat] mean and std.dev: (975.76, 939.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (273.26, 139.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 529, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3548) [M::mem_pestat] mean and std.dev: (713.80, 792.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (291, 536, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2277) [M::mem_pestat] mean and std.dev: (629.90, 512.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696510 sequences (100000246 bp) in 3.071 CPU sec, 3.394 real sec... [M::mem_process_seqs] Processed 696406 reads in 109.035 CPU sec, 3.787 real sec sorting block 24, #reads = 1076770 mem = 536880152 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283429, 33, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 376, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4096) [M::mem_pestat] mean and std.dev: (669.66, 742.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (273.83, 139.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (154, 620, 2473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7111) [M::mem_pestat] mean and std.dev: (1402.59, 1324.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9430) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 568, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5183) [M::mem_pestat] mean and std.dev: (904.00, 815.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696300 sequences (100000145 bp) in 3.279 CPU sec, 3.604 real sec... [M::mem_process_seqs] Processed 696510 reads in 109.087 CPU sec, 3.628 real sec sorting block 25, #reads = 1075227 mem = 536880318 peak = 807546880 [M::process] read 695804 sequences (100000044 bp) in 2.780 CPU sec, 2.979 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283464, 17, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 349, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3117) [M::mem_pestat] mean and std.dev: (617.08, 571.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (273.30, 138.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (452, 571, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020) [M::mem_pestat] mean and std.dev: (697.94, 444.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 640, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3385) [M::mem_pestat] mean and std.dev: (800.45, 765.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4418) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696316 sequences (100000251 bp) in 2.548 CPU sec, 2.753 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283580, 17, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 357, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3374) [M::mem_pestat] mean and std.dev: (723.94, 696.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (274.36, 138.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 582, 2164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6256) [M::mem_pestat] mean and std.dev: (668.60, 794.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 335, 726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1768) [M::mem_pestat] mean and std.dev: (512.66, 493.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696300 reads in 199.081 CPU sec, 6.404 real sec [M::mem_process_seqs] Processed 695804 reads in 127.646 CPU sec, 4.046 real sec sorting block 26, #reads = 1076399 mem = 536879752 peak = 807546880 [M::process] read 696008 sequences (100000033 bp) in 2.760 CPU sec, 2.980 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283921, 20, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 232, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2359) [M::mem_pestat] mean and std.dev: (408.74, 497.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (273.41, 138.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (413, 499, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1979) [M::mem_pestat] mean and std.dev: (615.15, 377.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 376, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1804) [M::mem_pestat] mean and std.dev: (390.38, 258.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696202 sequences (100000065 bp) in 2.623 CPU sec, 2.846 real sec... sorting block 27, #reads = 1077120 mem = 536878228 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283318, 17, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 298, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (216.27, 105.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (274.58, 139.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 554, 1643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4379) [M::mem_pestat] mean and std.dev: (1124.24, 1211.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 392, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2730) [M::mem_pestat] mean and std.dev: (497.67, 509.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696316 reads in 194.069 CPU sec, 6.222 real sec [M::mem_process_seqs] Processed 696008 reads in 131.134 CPU sec, 4.163 real sec [M::process] read 695530 sequences (100000160 bp) in 2.799 CPU sec, 2.938 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283812, 24, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 383, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322) [M::mem_pestat] mean and std.dev: (435.85, 249.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (275.45, 140.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 472, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3515) [M::mem_pestat] mean and std.dev: (572.86, 557.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4658) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 797, 1649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4507) [M::mem_pestat] mean and std.dev: (1005.34, 901.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 28, #reads = 1076892 mem = 536887406 peak = 807546880 [M::process] read 695512 sequences (100000217 bp) in 3.450 CPU sec, 3.745 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283236, 19, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 470, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (400.92, 256.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.49, 141.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1158, 1158, 1697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 2775) [M::mem_pestat] mean and std.dev: (1374.56, 505.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 402, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1711) [M::mem_pestat] mean and std.dev: (394.65, 255.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2203) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696202 reads in 200.914 CPU sec, 6.440 real sec [M::mem_process_seqs] Processed 695530 reads in 134.208 CPU sec, 4.259 real sec [M::process] read 694920 sequences (100000129 bp) in 2.661 CPU sec, 2.850 real sec... sorting block 29, #reads = 1075135 mem = 536876969 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283199, 20, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 294, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2934) [M::mem_pestat] mean and std.dev: (586.41, 692.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.46, 138.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 775, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4107) [M::mem_pestat] mean and std.dev: (861.16, 796.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 285, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (449.67, 434.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694622 sequences (100000086 bp) in 3.250 CPU sec, 3.488 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 282807, 28, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 370, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3108) [M::mem_pestat] mean and std.dev: (614.84, 503.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (277.52, 140.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (183, 407, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3369) [M::mem_pestat] mean and std.dev: (439.42, 458.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 392, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2277) [M::mem_pestat] mean and std.dev: (498.28, 371.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695512 reads in 202.236 CPU sec, 6.483 real sec [M::mem_process_seqs] Processed 694920 reads in 136.024 CPU sec, 4.323 real sec [M::process] read 694744 sequences (100000101 bp) in 2.235 CPU sec, 2.328 real sec... sorting block 30, #reads = 1075945 mem = 536881309 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 283084, 21, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 358, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1758) [M::mem_pestat] mean and std.dev: (514.74, 391.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (279.14, 141.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 519, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1912) [M::mem_pestat] mean and std.dev: (505.81, 299.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2462) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 400, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2354) [M::mem_pestat] mean and std.dev: (461.47, 340.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699260 sequences (100000086 bp) in 3.483 CPU sec, 3.732 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282999, 12, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 387, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2169) [M::mem_pestat] mean and std.dev: (440.19, 420.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 254, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (281.71, 143.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (461, 739, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3389) [M::mem_pestat] mean and std.dev: (797.91, 757.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 331, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739) [M::mem_pestat] mean and std.dev: (471.05, 349.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2250) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694622 reads in 204.943 CPU sec, 6.555 real sec sorting block 31, #reads = 1075015 mem = 536879258 peak = 807546880 [M::mem_process_seqs] Processed 694744 reads in 136.521 CPU sec, 4.324 real sec [M::process] read 700266 sequences (100000047 bp) in 2.929 CPU sec, 3.114 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284202, 20, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 246, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691) [M::mem_pestat] mean and std.dev: (303.37, 209.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (266.76, 136.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 1388, 2777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7831) [M::mem_pestat] mean and std.dev: (1575.05, 1446.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (333, 361, 1059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2511) [M::mem_pestat] mean and std.dev: (623.40, 512.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 32, #reads = 1075183 mem = 536881171 peak = 807546880 [M::process] read 699010 sequences (100000105 bp) in 3.426 CPU sec, 3.695 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284449, 28, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 333, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2569) [M::mem_pestat] mean and std.dev: (603.94, 582.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 235, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (262.59, 133.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (472, 597, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1687) [M::mem_pestat] mean and std.dev: (519.33, 292.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 273, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2244) [M::mem_pestat] mean and std.dev: (441.26, 400.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699260 reads in 210.116 CPU sec, 6.731 real sec [M::mem_process_seqs] Processed 700266 reads in 139.929 CPU sec, 4.428 real sec [M::process] read 698570 sequences (100000020 bp) in 3.194 CPU sec, 3.414 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284324, 14, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 354, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2020) [M::mem_pestat] mean and std.dev: (445.12, 408.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (265.41, 133.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 695, 1783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5291) [M::mem_pestat] mean and std.dev: (759.00, 898.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 477, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2912) [M::mem_pestat] mean and std.dev: (668.50, 540.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 33, #reads = 1075376 mem = 536881113 peak = 807546880 [M::process] read 697514 sequences (100000301 bp) in 3.085 CPU sec, 3.310 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284183, 28, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 532, 1631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4595) [M::mem_pestat] mean and std.dev: (778.11, 804.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (268.30, 136.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (530, 1031, 1827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4421) [M::mem_pestat] mean and std.dev: (1024.96, 850.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5718) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (319, 423, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2590) [M::mem_pestat] mean and std.dev: (537.44, 462.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699010 reads in 206.233 CPU sec, 6.626 real sec [M::mem_process_seqs] Processed 698570 reads in 138.819 CPU sec, 4.401 real sec [M::process] read 697402 sequences (100000012 bp) in 2.869 CPU sec, 3.048 real sec... sorting block 34, #reads = 1079019 mem = 536880946 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283774, 18, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 353, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168) [M::mem_pestat] mean and std.dev: (333.30, 170.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (270.10, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (187, 589, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2698) [M::mem_pestat] mean and std.dev: (691.00, 590.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 283, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3657) [M::mem_pestat] mean and std.dev: (680.69, 829.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696776 sequences (100000010 bp) in 3.567 CPU sec, 3.849 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283977, 27, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 362, 2058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5752) [M::mem_pestat] mean and std.dev: (821.00, 996.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.93, 137.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (766, 1089, 2704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6580) [M::mem_pestat] mean and std.dev: (1379.79, 1174.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 200, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355) [M::mem_pestat] mean and std.dev: (348.70, 314.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1750) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697514 reads in 204.197 CPU sec, 6.542 real sec [M::mem_process_seqs] Processed 697402 reads in 136.735 CPU sec, 4.335 real sec sorting block 35, #reads = 1078114 mem = 536880230 peak = 807546880 [M::process] read 696688 sequences (100000038 bp) in 2.794 CPU sec, 2.944 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283497, 17, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 331, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (347.32, 264.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (272.59, 137.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (505, 1442, 3190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8560) [M::mem_pestat] mean and std.dev: (2066.65, 2357.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 275, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691) [M::mem_pestat] mean and std.dev: (374.04, 235.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2175) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 36, #reads = 1077385 mem = 536877255 peak = 807546880 [M::process] read 696186 sequences (100000024 bp) in 3.283 CPU sec, 3.553 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283929, 17, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 381, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (439.81, 266.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (275.10, 139.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 624, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3269) [M::mem_pestat] mean and std.dev: (774.53, 596.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 349, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916) [M::mem_pestat] mean and std.dev: (534.86, 507.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696776 reads in 205.014 CPU sec, 6.561 real sec [M::mem_process_seqs] Processed 696688 reads in 137.532 CPU sec, 4.353 real sec [M::process] read 695858 sequences (100000067 bp) in 2.262 CPU sec, 2.359 real sec... sorting block 37, #reads = 1077261 mem = 536879493 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283167, 24, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 239, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (238.22, 101.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.45, 140.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 526, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2084) [M::mem_pestat] mean and std.dev: (548.23, 401.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 399, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327) [M::mem_pestat] mean and std.dev: (374.75, 176.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1682) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695570 sequences (100000273 bp) in 3.207 CPU sec, 3.481 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 283577, 23, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 467, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974) [M::mem_pestat] mean and std.dev: (495.10, 385.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (277.31, 140.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 840, 2010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5564) [M::mem_pestat] mean and std.dev: (891.95, 769.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 385, 2302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6442) [M::mem_pestat] mean and std.dev: (907.53, 1015.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696186 reads in 202.752 CPU sec, 6.488 real sec [M::mem_process_seqs] Processed 695858 reads in 132.105 CPU sec, 4.189 real sec [M::process] read 695612 sequences (100000074 bp) in 2.731 CPU sec, 2.872 real sec... sorting block 38, #reads = 1076037 mem = 536880503 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283266, 22, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 370, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2915) [M::mem_pestat] mean and std.dev: (649.07, 672.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (277.75, 140.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (149, 338, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (303.16, 252.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 498, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1581) [M::mem_pestat] mean and std.dev: (532.82, 402.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282963, 19, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 386, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2627) [M::mem_pestat] mean and std.dev: (592.44, 602.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (276.73, 141.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (519, 974, 3946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10800) [M::mem_pestat] mean and std.dev: (2438.89, 2736.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 357, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4116) [M::mem_pestat] mean and std.dev: (908.57, 1023.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695720 sequences (100000197 bp) in 4.063 CPU sec, 4.373 real sec... [M::mem_process_seqs] Processed 695570 reads in 203.755 CPU sec, 6.541 real sec [M::mem_process_seqs] Processed 695612 reads in 137.599 CPU sec, 4.368 real sec sorting block 39, #reads = 1077353 mem = 536882156 peak = 807546880 [M::process] read 695736 sequences (100000208 bp) in 3.336 CPU sec, 3.581 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 282883, 30, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 287, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179) [M::mem_pestat] mean and std.dev: (382.16, 235.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.40, 140.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 708, 1358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3476) [M::mem_pestat] mean and std.dev: (711.46, 523.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 349, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (314.97, 173.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283209, 22, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 531, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1500) [M::mem_pestat] mean and std.dev: (501.00, 339.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (274.45, 139.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (510, 794, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4191) [M::mem_pestat] mean and std.dev: (872.89, 611.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5418) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 379, 1799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4909) [M::mem_pestat] mean and std.dev: (1009.37, 1131.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 40, #reads = 1076084 mem = 536880831 peak = 807546880 [M::process] read 695946 sequences (100000192 bp) in 3.776 CPU sec, 4.162 real sec... [M::mem_process_seqs] Processed 695720 reads in 203.811 CPU sec, 6.547 real sec [M::mem_process_seqs] Processed 695736 reads in 137.483 CPU sec, 4.382 real sec [M::process] read 695564 sequences (100000038 bp) in 3.132 CPU sec, 3.360 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 283218, 15, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 584, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2892) [M::mem_pestat] mean and std.dev: (685.95, 633.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (274.03, 139.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (189, 628, 2086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5880) [M::mem_pestat] mean and std.dev: (1061.71, 1070.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 344, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187) [M::mem_pestat] mean and std.dev: (336.21, 211.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 41, #reads = 1075202 mem = 536878266 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283290, 17, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 355, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2098) [M::mem_pestat] mean and std.dev: (521.65, 427.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (274.74, 139.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 456, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3988) [M::mem_pestat] mean and std.dev: (731.00, 801.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 321, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691) [M::mem_pestat] mean and std.dev: (411.68, 360.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695802 sequences (100000036 bp) in 3.502 CPU sec, 3.821 real sec... [M::mem_process_seqs] Processed 695946 reads in 217.497 CPU sec, 7.042 real sec [M::mem_process_seqs] Processed 695564 reads in 139.492 CPU sec, 4.500 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 282909, 19, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 316, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (359.43, 238.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.87, 138.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (350, 2182, 2505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6815) [M::mem_pestat] mean and std.dev: (1530.05, 1130.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8970) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 306, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1446) [M::mem_pestat] mean and std.dev: (391.96, 287.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695802 reads in 114.229 CPU sec, 3.808 real sec sorting block 42, #reads = 1074840 mem = 536886742 peak = 807546880 [M::process] read 695696 sequences (100000087 bp) in 4.171 CPU sec, 4.568 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283260, 23, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 309, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (384.84, 331.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.26, 140.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (559, 1063, 3200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8482) [M::mem_pestat] mean and std.dev: (1517.74, 1287.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11123) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 402, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357) [M::mem_pestat] mean and std.dev: (501.78, 382.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695772 sequences (100000253 bp) in 3.319 CPU sec, 3.627 real sec... [M::mem_process_seqs] Processed 695696 reads in 117.471 CPU sec, 3.908 real sec sorting block 43, #reads = 1076249 mem = 536882194 peak = 807546880 [M::process] read 695372 sequences (100000026 bp) in 3.086 CPU sec, 3.334 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 282852, 20, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 465, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353) [M::mem_pestat] mean and std.dev: (405.05, 243.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (275.18, 139.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 763, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4463) [M::mem_pestat] mean and std.dev: (1028.15, 921.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5847) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 237, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (216.17, 52.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695772 reads in 120.006 CPU sec, 4.067 real sec [M::process] read 695470 sequences (100000119 bp) in 3.151 CPU sec, 3.382 real sec... sorting block 44, #reads = 1076001 mem = 536879708 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283014, 17, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 443, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4057) [M::mem_pestat] mean and std.dev: (820.21, 847.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (275.64, 139.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (522, 807, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3528) [M::mem_pestat] mean and std.dev: (926.82, 700.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 346, 2481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6965) [M::mem_pestat] mean and std.dev: (906.50, 1308.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695028 sequences (100000089 bp) in 3.110 CPU sec, 3.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283497, 29, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 1116, 2208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6288) [M::mem_pestat] mean and std.dev: (1239.63, 967.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.38, 140.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 746, 1374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3532) [M::mem_pestat] mean and std.dev: (1014.41, 841.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 274, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1439) [M::mem_pestat] mean and std.dev: (296.00, 192.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695372 reads in 210.839 CPU sec, 6.785 real sec [M::mem_process_seqs] Processed 695470 reads in 142.492 CPU sec, 4.537 real sec sorting block 45, #reads = 1075885 mem = 536879655 peak = 807546880 [M::process] read 694500 sequences (100000193 bp) in 3.185 CPU sec, 3.398 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283043, 15, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 473, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4217) [M::mem_pestat] mean and std.dev: (793.22, 658.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (278.04, 141.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 246, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3973) [M::mem_pestat] mean and std.dev: (783.20, 1048.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 358, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1599) [M::mem_pestat] mean and std.dev: (392.79, 291.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282506, 21, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 679, 1362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3688) [M::mem_pestat] mean and std.dev: (783.12, 718.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (278.98, 141.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 996, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3635) [M::mem_pestat] mean and std.dev: (833.58, 572.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (472, 630, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2734) [M::mem_pestat] mean and std.dev: (682.10, 400.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694448 sequences (100000163 bp) in 3.122 CPU sec, 3.400 real sec... [M::mem_process_seqs] Processed 695028 reads in 206.928 CPU sec, 6.661 real sec sorting block 46, #reads = 1075377 mem = 536879541 peak = 807546880 [M::mem_process_seqs] Processed 694500 reads in 139.252 CPU sec, 4.429 real sec [M::process] read 693910 sequences (100000140 bp) in 3.236 CPU sec, 3.485 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282665, 35, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 388, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4036) [M::mem_pestat] mean and std.dev: (739.85, 784.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (280.83, 142.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (337, 851, 2531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6919) [M::mem_pestat] mean and std.dev: (1191.44, 1262.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 375, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1786) [M::mem_pestat] mean and std.dev: (439.82, 291.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 47, #reads = 1075862 mem = 536877427 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 282282, 28, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 312, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1748) [M::mem_pestat] mean and std.dev: (496.22, 373.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (280.83, 141.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (441, 723, 2572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6834) [M::mem_pestat] mean and std.dev: (1881.14, 2153.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 355, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1988) [M::mem_pestat] mean and std.dev: (557.40, 475.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696318 sequences (100000112 bp) in 3.048 CPU sec, 3.293 real sec... [M::mem_process_seqs] Processed 694448 reads in 206.077 CPU sec, 6.638 real sec [M::mem_process_seqs] Processed 693910 reads in 139.270 CPU sec, 4.426 real sec [M::process] read 700424 sequences (100000249 bp) in 3.247 CPU sec, 3.504 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 283368, 16, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 428, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157) [M::mem_pestat] mean and std.dev: (361.07, 183.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (275.50, 140.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (467, 1007, 1406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3284) [M::mem_pestat] mean and std.dev: (859.56, 494.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 336, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937) [M::mem_pestat] mean and std.dev: (512.69, 437.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2505) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 48, #reads = 1074747 mem = 536876664 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285520, 16, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 337, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2136) [M::mem_pestat] mean and std.dev: (438.65, 340.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (262.32, 134.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 881, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4186) [M::mem_pestat] mean and std.dev: (797.67, 712.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 432, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723) [M::mem_pestat] mean and std.dev: (389.88, 236.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699902 sequences (100000197 bp) in 3.389 CPU sec, 3.682 real sec... [M::mem_process_seqs] Processed 696318 reads in 206.947 CPU sec, 6.658 real sec [M::mem_process_seqs] Processed 700424 reads in 138.897 CPU sec, 4.411 real sec sorting block 49, #reads = 1074310 mem = 536880738 peak = 807546880 [M::process] read 699166 sequences (100000265 bp) in 3.714 CPU sec, 4.032 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 284969, 28, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 307, 1246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3346) [M::mem_pestat] mean and std.dev: (567.27, 573.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (264.35, 135.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 912, 1527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4079) [M::mem_pestat] mean and std.dev: (918.58, 733.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 398, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (335.75, 192.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699902 reads in 116.008 CPU sec, 3.851 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284046, 23, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 215, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (279.41, 175.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (266.84, 137.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 765, 1756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4474) [M::mem_pestat] mean and std.dev: (1043.13, 862.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 542, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2222) [M::mem_pestat] mean and std.dev: (562.16, 430.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698666 sequences (100000247 bp) in 3.353 CPU sec, 3.626 real sec... [M::mem_process_seqs] Processed 699166 reads in 115.428 CPU sec, 3.826 real sec sorting block 50, #reads = 1080233 mem = 536879676 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284357, 24, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 321, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954) [M::mem_pestat] mean and std.dev: (422.19, 366.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (269.30, 138.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 833, 1671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4523) [M::mem_pestat] mean and std.dev: (1078.35, 999.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 322, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3199) [M::mem_pestat] mean and std.dev: (568.93, 457.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698446 sequences (100000166 bp) in 3.458 CPU sec, 3.791 real sec... [M::mem_process_seqs] Processed 698666 reads in 112.982 CPU sec, 3.761 real sec sorting block 51, #reads = 1079691 mem = 536883739 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 283721, 25, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 447, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2535) [M::mem_pestat] mean and std.dev: (578.37, 551.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (269.65, 138.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (337, 668, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3067) [M::mem_pestat] mean and std.dev: (699.36, 463.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3977) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 320, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2455) [M::mem_pestat] mean and std.dev: (492.00, 458.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697540 sequences (100000008 bp) in 3.339 CPU sec, 3.563 real sec... [M::mem_process_seqs] Processed 698446 reads in 114.920 CPU sec, 3.842 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 283465, 34, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 283, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357) [M::mem_pestat] mean and std.dev: (276.44, 216.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (272.72, 140.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (544, 1883, 2187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5473) [M::mem_pestat] mean and std.dev: (1331.67, 1184.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 376, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4106) [M::mem_pestat] mean and std.dev: (752.08, 777.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5418) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697466 sequences (100000045 bp) in 3.106 CPU sec, 3.364 real sec... [M::mem_process_seqs] Processed 697540 reads in 115.626 CPU sec, 3.829 real sec sorting block 52, #reads = 1080334 mem = 536878956 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283322, 25, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 334, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (380.68, 267.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (272.52, 139.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 336, 2692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7994) [M::mem_pestat] mean and std.dev: (1317.16, 1875.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 471, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1760) [M::mem_pestat] mean and std.dev: (563.41, 411.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2266) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697034 sequences (100000259 bp) in 3.406 CPU sec, 3.681 real sec... [M::mem_process_seqs] Processed 697466 reads in 116.134 CPU sec, 3.852 real sec sorting block 53, #reads = 1078242 mem = 536878033 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283447, 28, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 302, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326) [M::mem_pestat] mean and std.dev: (326.86, 219.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (273.87, 140.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 336, 1943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5233) [M::mem_pestat] mean and std.dev: (603.54, 716.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 352, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2821) [M::mem_pestat] mean and std.dev: (385.72, 269.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3685) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697920 sequences (100000006 bp) in 3.726 CPU sec, 4.032 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 283545, 24, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 273, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1890) [M::mem_pestat] mean and std.dev: (415.64, 412.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (270.73, 138.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (401, 747, 1892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4874) [M::mem_pestat] mean and std.dev: (1077.78, 916.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (320, 761, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3278) [M::mem_pestat] mean and std.dev: (862.88, 808.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696382 sequences (100000211 bp) in 3.232 CPU sec, 3.460 real sec... [M::mem_process_seqs] Processed 697920 reads in 116.461 CPU sec, 3.746 real sec sorting block 54, #reads = 1078103 mem = 536879974 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283435, 21, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 306, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2821) [M::mem_pestat] mean and std.dev: (626.43, 661.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (275.70, 140.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (359, 490, 1102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (679.38, 471.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3331) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 320, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528) [M::mem_pestat] mean and std.dev: (413.96, 366.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695972 sequences (100000219 bp) in 3.536 CPU sec, 3.798 real sec... [M::mem_process_seqs] Processed 697034 reads in 344.604 CPU sec, 11.203 real sec [M::mem_process_seqs] Processed 696382 reads in 115.170 CPU sec, 3.714 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283277, 29, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 293, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1584) [M::mem_pestat] mean and std.dev: (370.42, 239.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.14, 140.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (288, 523, 2546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7062) [M::mem_pestat] mean and std.dev: (1188.79, 1202.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 385, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4001) [M::mem_pestat] mean and std.dev: (601.93, 678.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696180 sequences (100000074 bp) in 3.290 CPU sec, 3.785 real sec... sorting block 55, #reads = 1078242 mem = 536879191 peak = 807546880 [M::mem_process_seqs] Processed 695972 reads in 115.282 CPU sec, 4.006 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 283599, 28, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 472, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483) [M::mem_pestat] mean and std.dev: (766.43, 692.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.92, 140.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 787, 2746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7874) [M::mem_pestat] mean and std.dev: (1353.04, 1492.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (347, 772, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909) [M::mem_pestat] mean and std.dev: (772.72, 583.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695934 sequences (100000267 bp) in 3.579 CPU sec, 3.896 real sec... [M::mem_process_seqs] Processed 696180 reads in 115.261 CPU sec, 4.323 real sec sorting block 56, #reads = 1077243 mem = 536877610 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282841, 25, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 234, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2388) [M::mem_pestat] mean and std.dev: (515.27, 571.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (274.57, 139.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 786, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4225) [M::mem_pestat] mean and std.dev: (822.78, 765.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 421, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2672) [M::mem_pestat] mean and std.dev: (525.52, 501.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696014 sequences (100000225 bp) in 3.511 CPU sec, 4.138 real sec... [M::mem_process_seqs] Processed 695934 reads in 118.146 CPU sec, 4.616 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283293, 19, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 449, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1697) [M::mem_pestat] mean and std.dev: (522.82, 334.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (274.70, 140.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (753, 1103, 2698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6588) [M::mem_pestat] mean and std.dev: (1646.33, 1664.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 353, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416) [M::mem_pestat] mean and std.dev: (397.93, 251.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695998 sequences (100000056 bp) in 3.456 CPU sec, 3.739 real sec... [M::mem_process_seqs] Processed 696014 reads in 115.112 CPU sec, 3.830 real sec sorting block 57, #reads = 1076851 mem = 536880360 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283407, 23, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 174, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (249.08, 177.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (274.46, 140.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (753, 1467, 3485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8949) [M::mem_pestat] mean and std.dev: (2158.04, 1558.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (316, 493, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3676) [M::mem_pestat] mean and std.dev: (828.72, 763.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695920 sequences (100000054 bp) in 3.692 CPU sec, 4.040 real sec... [M::mem_process_seqs] Processed 695998 reads in 115.834 CPU sec, 4.389 real sec sorting block 58, #reads = 1075198 mem = 536886636 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282719, 9, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 272, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2960) [M::mem_pestat] mean and std.dev: (617.55, 714.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (273.73, 138.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 339, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (314.45, 196.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695920 reads in 115.011 CPU sec, 3.844 real sec [M::process] read 695760 sequences (100000135 bp) in 3.786 CPU sec, 4.140 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283091, 25, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 375, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (312.52, 161.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (274.45, 139.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 399, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2575) [M::mem_pestat] mean and std.dev: (758.04, 759.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 675, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2880) [M::mem_pestat] mean and std.dev: (730.06, 414.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695760 reads in 115.359 CPU sec, 3.837 real sec [M::process] read 695640 sequences (100000003 bp) in 4.458 CPU sec, 4.902 real sec... sorting block 59, #reads = 1076113 mem = 536880022 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282818, 33, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 443, 2916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8232) [M::mem_pestat] mean and std.dev: (1573.36, 2116.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (274.74, 138.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (150, 370, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1560) [M::mem_pestat] mean and std.dev: (370.39, 296.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2030) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 467, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2882) [M::mem_pestat] mean and std.dev: (615.93, 526.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695296 sequences (100000084 bp) in 3.512 CPU sec, 3.809 real sec... [M::mem_process_seqs] Processed 695640 reads in 117.039 CPU sec, 4.466 real sec sorting block 60, #reads = 1075646 mem = 536880424 peak = 807546880 [M::process] read 695212 sequences (100000151 bp) in 2.788 CPU sec, 3.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 283711, 17, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 434, 911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2045) [M::mem_pestat] mean and std.dev: (595.00, 468.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (275.48, 138.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (254, 552, 3814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10934) [M::mem_pestat] mean and std.dev: (1609.06, 1857.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 420, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4406) [M::mem_pestat] mean and std.dev: (766.39, 863.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695296 reads in 116.230 CPU sec, 3.909 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283331, 23, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 319, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (334.67, 170.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (275.61, 138.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 681, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3012) [M::mem_pestat] mean and std.dev: (693.50, 580.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3913) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 328, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1719) [M::mem_pestat] mean and std.dev: (408.57, 304.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695212 reads in 112.273 CPU sec, 3.758 real sec [M::process] read 695122 sequences (100000292 bp) in 3.893 CPU sec, 4.348 real sec... sorting block 61, #reads = 1076005 mem = 536879527 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283262, 42, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 308, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2077) [M::mem_pestat] mean and std.dev: (397.29, 329.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.85, 137.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 1002, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3745) [M::mem_pestat] mean and std.dev: (836.18, 663.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (318, 433, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1800) [M::mem_pestat] mean and std.dev: (616.50, 446.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695122 reads in 114.235 CPU sec, 4.027 real sec [M::process] read 695074 sequences (100000058 bp) in 4.382 CPU sec, 4.806 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283200, 11, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 693, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2797) [M::mem_pestat] mean and std.dev: (798.44, 732.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (276.00, 138.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (713, 874, 3491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9047) [M::mem_pestat] mean and std.dev: (1752.91, 1385.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 389, 1374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3736) [M::mem_pestat] mean and std.dev: (727.53, 694.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 62, #reads = 1076551 mem = 536878349 peak = 807546880 [M::mem_process_seqs] Processed 695074 reads in 111.680 CPU sec, 3.732 real sec [M::process] read 694552 sequences (100000055 bp) in 4.136 CPU sec, 4.551 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282823, 21, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 509, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (425.83, 247.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (278.21, 139.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (228, 716, 2483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6993) [M::mem_pestat] mean and std.dev: (1278.33, 1521.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 337, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2237) [M::mem_pestat] mean and std.dev: (523.97, 425.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 694264 sequences (100000186 bp) in 3.738 CPU sec, 4.085 real sec... [M::mem_process_seqs] Processed 694552 reads in 115.217 CPU sec, 4.161 real sec sorting block 63, #reads = 1075190 mem = 536879465 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283089, 15, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 859, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3633) [M::mem_pestat] mean and std.dev: (829.00, 732.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (280.13, 141.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 305, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2948) [M::mem_pestat] mean and std.dev: (666.53, 617.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 443, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3504) [M::mem_pestat] mean and std.dev: (727.47, 552.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694264 reads in 110.882 CPU sec, 3.738 real sec [M::process] read 694504 sequences (100000172 bp) in 3.895 CPU sec, 4.260 real sec... sorting block 64, #reads = 1075112 mem = 536879189 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 282428, 18, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 269, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1685) [M::mem_pestat] mean and std.dev: (399.22, 358.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (280.65, 142.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (96, 516, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3858) [M::mem_pestat] mean and std.dev: (647.94, 733.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (328, 402, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (375.66, 157.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1504) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704572 sequences (100000000 bp) in 3.320 CPU sec, 3.534 real sec... [M::mem_process_seqs] Processed 694504 reads in 118.164 CPU sec, 3.969 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 286059, 14, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 321, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1984) [M::mem_pestat] mean and std.dev: (442.79, 453.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (249.84, 129.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 898, 1722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4970) [M::mem_pestat] mean and std.dev: (1045.43, 1047.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 316, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2058) [M::mem_pestat] mean and std.dev: (428.21, 351.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2688) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704572 reads in 122.246 CPU sec, 4.053 real sec [M::process] read 702446 sequences (100000042 bp) in 4.304 CPU sec, 4.749 real sec... sorting block 65, #reads = 1075514 mem = 536883955 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285883, 31, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 285, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (312.62, 189.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 226, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (256.89, 133.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 1060, 2724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7650) [M::mem_pestat] mean and std.dev: (1515.14, 1874.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 368, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4371) [M::mem_pestat] mean and std.dev: (862.19, 1028.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702446 reads in 114.452 CPU sec, 4.023 real sec [M::process] read 700872 sequences (100000157 bp) in 4.257 CPU sec, 4.654 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285646, 32, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 275, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (273.69, 154.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 230, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (260.71, 135.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 898, 2222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5938) [M::mem_pestat] mean and std.dev: (1190.62, 1115.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7796) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 366, 2795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8009) [M::mem_pestat] mean and std.dev: (1454.48, 1952.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 66, #reads = 1074595 mem = 536879006 peak = 807546880 [M::mem_process_seqs] Processed 700872 reads in 110.407 CPU sec, 3.885 real sec [M::process] read 700664 sequences (100000213 bp) in 4.340 CPU sec, 4.809 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285184, 23, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 363, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1766) [M::mem_pestat] mean and std.dev: (376.88, 282.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 232, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (262.29, 135.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (367, 567, 1288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3130) [M::mem_pestat] mean and std.dev: (648.35, 622.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4051) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 411, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372) [M::mem_pestat] mean and std.dev: (463.56, 231.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700664 reads in 113.992 CPU sec, 3.986 real sec [M::process] read 699664 sequences (100000282 bp) in 4.169 CPU sec, 4.613 real sec... sorting block 67, #reads = 1079462 mem = 536881971 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285147, 37, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 750, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2949) [M::mem_pestat] mean and std.dev: (744.23, 626.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (266.73, 138.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (539, 1002, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3944) [M::mem_pestat] mean and std.dev: (1062.00, 864.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5079) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 360, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1713) [M::mem_pestat] mean and std.dev: (375.76, 287.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699222 sequences (100000188 bp) in 3.816 CPU sec, 4.167 real sec... [M::mem_process_seqs] Processed 699664 reads in 118.769 CPU sec, 4.212 real sec sorting block 68, #reads = 1078486 mem = 536880024 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 285329, 24, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 539, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3058) [M::mem_pestat] mean and std.dev: (646.80, 583.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (268.11, 139.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 579, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4658) [M::mem_pestat] mean and std.dev: (1150.42, 1222.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 329, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2461) [M::mem_pestat] mean and std.dev: (550.39, 494.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698618 sequences (100000031 bp) in 3.437 CPU sec, 3.732 real sec... [M::mem_process_seqs] Processed 699222 reads in 126.661 CPU sec, 4.207 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284774, 28, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 709, 1830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5022) [M::mem_pestat] mean and std.dev: (879.47, 800.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (270.69, 141.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 460, 2178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6290) [M::mem_pestat] mean and std.dev: (912.08, 1180.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 383, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2169) [M::mem_pestat] mean and std.dev: (476.95, 366.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698096 sequences (100000139 bp) in 3.883 CPU sec, 4.319 real sec... [M::mem_process_seqs] Processed 698618 reads in 115.794 CPU sec, 4.126 real sec sorting block 69, #reads = 1075316 mem = 536877957 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 285002, 24, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 399, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2325) [M::mem_pestat] mean and std.dev: (617.34, 543.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (272.16, 141.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (467, 753, 2157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5537) [M::mem_pestat] mean and std.dev: (1009.38, 737.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (364.78, 155.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698096 reads in 116.308 CPU sec, 3.884 real sec [M::process] read 697784 sequences (100000167 bp) in 4.383 CPU sec, 4.841 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284046, 33, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 314, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1760) [M::mem_pestat] mean and std.dev: (451.49, 385.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (272.94, 142.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (316, 366, 4110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11698) [M::mem_pestat] mean and std.dev: (1479.33, 1948.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 433, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3819) [M::mem_pestat] mean and std.dev: (626.05, 727.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 70, #reads = 1077059 mem = 536879816 peak = 807546880 [M::mem_process_seqs] Processed 697784 reads in 120.451 CPU sec, 4.003 real sec [M::process] read 697562 sequences (100000108 bp) in 4.406 CPU sec, 4.866 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283805, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 355, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (327.84, 143.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.98, 142.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 354, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533) [M::mem_pestat] mean and std.dev: (429.50, 417.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 387, 2131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5877) [M::mem_pestat] mean and std.dev: (937.27, 999.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7750) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697562 reads in 119.036 CPU sec, 4.124 real sec [M::process] read 697000 sequences (100000192 bp) in 4.144 CPU sec, 4.633 real sec... sorting block 71, #reads = 1077105 mem = 536878662 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283462, 30, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 324, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1491) [M::mem_pestat] mean and std.dev: (414.44, 317.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (275.11, 143.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 376, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336) [M::mem_pestat] mean and std.dev: (334.56, 199.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 337, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434) [M::mem_pestat] mean and std.dev: (375.17, 218.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696718 sequences (100000299 bp) in 3.600 CPU sec, 3.958 real sec... [M::mem_process_seqs] Processed 697000 reads in 119.231 CPU sec, 4.175 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283262, 27, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 333, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985) [M::mem_pestat] mean and std.dev: (354.25, 211.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (275.42, 142.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 891, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4021) [M::mem_pestat] mean and std.dev: (846.19, 738.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5308) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 383, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2261) [M::mem_pestat] mean and std.dev: (482.69, 360.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 72, #reads = 1075323 mem = 536877750 peak = 807546880 [M::mem_process_seqs] Processed 696718 reads in 117.119 CPU sec, 3.973 real sec [M::process] read 696638 sequences (100000238 bp) in 4.281 CPU sec, 4.759 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 283412, 21, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 399, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2285) [M::mem_pestat] mean and std.dev: (462.32, 347.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (274.69, 141.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 468, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2872) [M::mem_pestat] mean and std.dev: (406.53, 328.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 908, 2078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5624) [M::mem_pestat] mean and std.dev: (1292.30, 1316.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696624 sequences (100000229 bp) in 3.592 CPU sec, 4.227 real sec... [M::mem_process_seqs] Processed 696638 reads in 121.161 CPU sec, 4.530 real sec sorting block 73, #reads = 1073372 mem = 536880351 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283648, 24, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 213, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2657) [M::mem_pestat] mean and std.dev: (549.26, 632.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (274.95, 141.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 1274, 1569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3907) [M::mem_pestat] mean and std.dev: (1225.38, 1049.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 535, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769) [M::mem_pestat] mean and std.dev: (483.69, 309.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696556 sequences (100000167 bp) in 3.409 CPU sec, 3.739 real sec... [M::mem_process_seqs] Processed 696624 reads in 120.633 CPU sec, 4.026 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283087, 19, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 238, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (256.22, 176.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (273.73, 140.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 752, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (614.47, 514.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 373, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968) [M::mem_pestat] mean and std.dev: (344.39, 171.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 74, #reads = 1073403 mem = 536879510 peak = 807546880 [M::mem_process_seqs] Processed 696556 reads in 118.427 CPU sec, 4.094 real sec [M::process] read 697076 sequences (100000287 bp) in 4.078 CPU sec, 4.507 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284067, 21, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 294, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331) [M::mem_pestat] mean and std.dev: (342.00, 242.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (273.66, 141.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (196, 1340, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5104) [M::mem_pestat] mean and std.dev: (769.29, 653.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 379, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2535) [M::mem_pestat] mean and std.dev: (627.94, 621.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695916 sequences (100000134 bp) in 3.714 CPU sec, 4.028 real sec... [M::mem_process_seqs] Processed 697076 reads in 120.252 CPU sec, 4.211 real sec sorting block 75, #reads = 1073574 mem = 536880988 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 282765, 11, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 354, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2510) [M::mem_pestat] mean and std.dev: (473.40, 396.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (276.24, 141.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (424, 734, 2216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5800) [M::mem_pestat] mean and std.dev: (989.70, 1130.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7592) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 694, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3833) [M::mem_pestat] mean and std.dev: (717.71, 620.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695916 reads in 123.514 CPU sec, 4.155 real sec [M::process] read 696134 sequences (100000235 bp) in 4.436 CPU sec, 4.886 real sec... sorting block 76, #reads = 1073689 mem = 536879711 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283340, 24, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 485, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1387) [M::mem_pestat] mean and std.dev: (500.28, 276.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (273.71, 139.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 477, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3684) [M::mem_pestat] mean and std.dev: (641.30, 763.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 354, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (421.70, 306.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696134 reads in 118.247 CPU sec, 4.058 real sec [M::process] read 696360 sequences (100000184 bp) in 3.876 CPU sec, 4.189 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283495, 28, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 507, 2036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5636) [M::mem_pestat] mean and std.dev: (888.06, 873.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.94, 138.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 314, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2485) [M::mem_pestat] mean and std.dev: (487.07, 467.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 423, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2370) [M::mem_pestat] mean and std.dev: (460.89, 309.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696360 reads in 115.676 CPU sec, 3.842 real sec [M::process] read 696318 sequences (100000114 bp) in 4.869 CPU sec, 5.356 real sec... sorting block 77, #reads = 1071803 mem = 536880010 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283221, 27, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 274, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (344.72, 225.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.99, 136.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (95, 592, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3122) [M::mem_pestat] mean and std.dev: (609.04, 621.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 576, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (469.81, 257.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1605) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696318 reads in 119.099 CPU sec, 4.083 real sec [M::process] read 696368 sequences (100000006 bp) in 3.778 CPU sec, 4.220 real sec... sorting block 78, #reads = 1072602 mem = 536878536 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282894, 17, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 438, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279) [M::mem_pestat] mean and std.dev: (557.94, 456.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.74, 138.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (102, 359, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (582.29, 521.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 369, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (340.23, 183.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696684 sequences (100000064 bp) in 3.266 CPU sec, 3.593 real sec... [M::mem_process_seqs] Processed 696368 reads in 119.423 CPU sec, 4.098 real sec [M::process] read 696862 sequences (100000220 bp) in 3.202 CPU sec, 3.423 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283703, 32, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 340, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1506) [M::mem_pestat] mean and std.dev: (462.13, 385.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (272.87, 139.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (324, 606, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2646) [M::mem_pestat] mean and std.dev: (658.79, 496.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3420) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 399, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3578) [M::mem_pestat] mean and std.dev: (736.87, 737.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 79, #reads = 1071883 mem = 536877674 peak = 807546880 [M::process] read 703404 sequences (100000064 bp) in 2.920 CPU sec, 3.080 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283793, 28, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 499, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2856) [M::mem_pestat] mean and std.dev: (688.12, 572.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (274.17, 141.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (267, 654, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279) [M::mem_pestat] mean and std.dev: (815.14, 678.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4283) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 284, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1813) [M::mem_pestat] mean and std.dev: (454.00, 397.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696684 reads in 223.610 CPU sec, 7.165 real sec [M::mem_process_seqs] Processed 696862 reads in 153.884 CPU sec, 4.911 real sec [M::process] read 704036 sequences (100000111 bp) in 2.765 CPU sec, 2.919 real sec... sorting block 80, #reads = 1073715 mem = 536883777 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285999, 29, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 324, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2856) [M::mem_pestat] mean and std.dev: (639.27, 648.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (251.28, 128.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 839, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3741) [M::mem_pestat] mean and std.dev: (864.10, 718.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 308, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2621) [M::mem_pestat] mean and std.dev: (498.69, 503.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702508 sequences (100000199 bp) in 3.914 CPU sec, 4.155 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286275, 13, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 354, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (524.90, 494.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (250.50, 127.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 576, 2447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6943) [M::mem_pestat] mean and std.dev: (939.50, 933.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 374, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957) [M::mem_pestat] mean and std.dev: (488.13, 414.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2536) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703404 reads in 237.745 CPU sec, 7.627 real sec [M::mem_process_seqs] Processed 704036 reads in 158.272 CPU sec, 5.060 real sec [M::process] read 701626 sequences (100000048 bp) in 2.525 CPU sec, 2.610 real sec... sorting block 81, #reads = 1072446 mem = 536881034 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286101, 16, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 360, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1291) [M::mem_pestat] mean and std.dev: (384.61, 231.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 226, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (255.51, 130.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (326, 573, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3080) [M::mem_pestat] mean and std.dev: (626.07, 627.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3998) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 341, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2464) [M::mem_pestat] mean and std.dev: (597.20, 545.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701784 sequences (100000157 bp) in 3.189 CPU sec, 3.415 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285951, 20, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 202, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (219.23, 103.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (258.00, 132.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 419, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2724) [M::mem_pestat] mean and std.dev: (602.42, 739.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (279, 602, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3186) [M::mem_pestat] mean and std.dev: (758.62, 620.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701834 sequences (100000213 bp) in 1.894 CPU sec, 1.902 real sec... [M::mem_process_seqs] Processed 702508 reads in 234.581 CPU sec, 7.746 real sec sorting block 82, #reads = 1075526 mem = 536882270 peak = 807546880 [M::mem_process_seqs] Processed 701626 reads in 149.529 CPU sec, 4.763 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 286196, 20, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 385, 4070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11754) [M::mem_pestat] mean and std.dev: (1712.95, 2286.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 228, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (257.93, 132.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (531, 1238, 1976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4866) [M::mem_pestat] mean and std.dev: (1133.33, 769.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 297, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2058) [M::mem_pestat] mean and std.dev: (298.69, 154.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 83, #reads = 1075845 mem = 536880580 peak = 807546880 [M::process] read 700164 sequences (100000187 bp) in 3.820 CPU sec, 4.212 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285965, 13, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 451, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3031) [M::mem_pestat] mean and std.dev: (595.32, 603.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 227, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (257.23, 132.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 969, 2668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7380) [M::mem_pestat] mean and std.dev: (1270.62, 1005.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9736) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 617, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1927) [M::mem_pestat] mean and std.dev: (507.92, 301.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701784 reads in 230.619 CPU sec, 7.380 real sec [M::process] read 699712 sequences (100000278 bp) in 2.482 CPU sec, 2.551 real sec... [M::mem_process_seqs] Processed 701834 reads in 156.485 CPU sec, 5.304 real sec sorting block 84, #reads = 1075052 mem = 536889694 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286057, 20, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 465, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420) [M::mem_pestat] mean and std.dev: (602.98, 502.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (263.58, 136.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 318, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4250) [M::mem_pestat] mean and std.dev: (691.75, 730.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5606) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 259, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1274) [M::mem_pestat] mean and std.dev: (330.80, 180.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699022 sequences (100000186 bp) in 3.285 CPU sec, 3.530 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285095, 23, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 354, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (341.84, 174.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (266.12, 137.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 938, 2649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7055) [M::mem_pestat] mean and std.dev: (1619.13, 1821.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 382, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (393.52, 205.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698926 sequences (100000175 bp) in 1.962 CPU sec, 1.970 real sec... [M::mem_process_seqs] Processed 700164 reads in 226.379 CPU sec, 7.578 real sec [M::mem_process_seqs] Processed 699712 reads in 148.628 CPU sec, 4.724 real sec sorting block 85, #reads = 1074299 mem = 536885090 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285415, 25, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 393, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140) [M::mem_pestat] mean and std.dev: (328.30, 150.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (267.34, 137.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (584, 2514, 3578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9566) [M::mem_pestat] mean and std.dev: (2202.76, 1848.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12560) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 321, 1576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4260) [M::mem_pestat] mean and std.dev: (954.17, 1035.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698110 sequences (100000008 bp) in 3.317 CPU sec, 3.574 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 285157, 27, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 494, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3349) [M::mem_pestat] mean and std.dev: (698.96, 714.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (269.40, 139.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 536, 2220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6272) [M::mem_pestat] mean and std.dev: (1038.41, 1054.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 534, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1846) [M::mem_pestat] mean and std.dev: (535.66, 353.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699022 reads in 213.947 CPU sec, 6.813 real sec [M::process] read 697738 sequences (100000160 bp) in 2.675 CPU sec, 2.726 real sec... sorting block 86, #reads = 1075471 mem = 536877993 peak = 807546880 [M::mem_process_seqs] Processed 698926 reads in 143.729 CPU sec, 4.864 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283823, 15, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 257, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3644) [M::mem_pestat] mean and std.dev: (681.87, 871.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (271.42, 140.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 788, 1662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4210) [M::mem_pestat] mean and std.dev: (1068.29, 1015.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 489, 1500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4044) [M::mem_pestat] mean and std.dev: (793.58, 674.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5316) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 87, #reads = 1074621 mem = 536877609 peak = 807546880 [M::process] read 696864 sequences (100000213 bp) in 3.744 CPU sec, 4.206 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284125, 24, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 274, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1487) [M::mem_pestat] mean and std.dev: (251.63, 111.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (272.94, 141.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (396, 733, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2589) [M::mem_pestat] mean and std.dev: (637.90, 418.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 338, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144) [M::mem_pestat] mean and std.dev: (498.67, 421.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698110 reads in 215.084 CPU sec, 7.186 real sec [M::mem_process_seqs] Processed 697738 reads in 145.463 CPU sec, 4.595 real sec [M::process] read 697308 sequences (100000254 bp) in 2.970 CPU sec, 3.140 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283681, 30, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 316, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (319.25, 226.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (273.49, 141.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 568, 1523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4127) [M::mem_pestat] mean and std.dev: (802.21, 750.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 429, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2313) [M::mem_pestat] mean and std.dev: (681.09, 515.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 88, #reads = 1073391 mem = 536879679 peak = 807546880 [M::process] read 696580 sequences (100000119 bp) in 3.592 CPU sec, 3.847 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283922, 42, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 599, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3229) [M::mem_pestat] mean and std.dev: (717.68, 662.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (272.56, 140.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (676, 902, 2537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6259) [M::mem_pestat] mean and std.dev: (1400.92, 1361.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8120) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 328, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5133) [M::mem_pestat] mean and std.dev: (937.12, 1110.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696864 reads in 215.974 CPU sec, 6.905 real sec [M::mem_process_seqs] Processed 697308 reads in 145.223 CPU sec, 4.590 real sec [M::process] read 696292 sequences (100000100 bp) in 3.419 CPU sec, 3.673 real sec... sorting block 89, #reads = 1072408 mem = 536878515 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 283382, 36, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 417, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2937) [M::mem_pestat] mean and std.dev: (731.33, 747.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (274.59, 141.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 826, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (651.50, 444.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2955) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 590, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2987) [M::mem_pestat] mean and std.dev: (656.54, 661.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283004, 17, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 470, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3053) [M::mem_pestat] mean and std.dev: (604.87, 480.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (275.06, 141.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (318, 378, 2265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6159) [M::mem_pestat] mean and std.dev: (731.13, 788.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 341, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3484) [M::mem_pestat] mean and std.dev: (570.66, 683.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696812 sequences (100000066 bp) in 3.875 CPU sec, 4.220 real sec... [M::mem_process_seqs] Processed 696580 reads in 212.559 CPU sec, 6.816 real sec [M::mem_process_seqs] Processed 696292 reads in 143.990 CPU sec, 4.561 real sec sorting block 90, #reads = 1073418 mem = 536879135 peak = 807546880 [M::process] read 696432 sequences (100000025 bp) in 3.545 CPU sec, 3.883 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283769, 35, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 315, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2770) [M::mem_pestat] mean and std.dev: (592.35, 607.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (275.14, 142.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (387, 1359, 2982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8172) [M::mem_pestat] mean and std.dev: (1518.47, 1302.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 443, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2994) [M::mem_pestat] mean and std.dev: (690.36, 622.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3908) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283366, 15, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 361, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1918) [M::mem_pestat] mean and std.dev: (540.88, 441.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (274.82, 142.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (302, 601, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2519) [M::mem_pestat] mean and std.dev: (592.93, 436.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 764, 2925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8177) [M::mem_pestat] mean and std.dev: (1619.71, 1839.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 91, #reads = 1072629 mem = 536881078 peak = 807546880 [M::mem_process_seqs] Processed 696812 reads in 208.875 CPU sec, 6.716 real sec [M::process] read 696514 sequences (100000130 bp) in 4.043 CPU sec, 4.458 real sec... [M::mem_process_seqs] Processed 696432 reads in 136.939 CPU sec, 4.367 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283240, 26, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 383, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2686) [M::mem_pestat] mean and std.dev: (500.34, 419.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (276.47, 144.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (473, 980, 1976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4982) [M::mem_pestat] mean and std.dev: (1128.04, 855.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (328, 376, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2167) [M::mem_pestat] mean and std.dev: (489.82, 356.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696384 sequences (100000242 bp) in 3.411 CPU sec, 3.750 real sec... [M::mem_process_seqs] Processed 696514 reads in 115.500 CPU sec, 3.818 real sec sorting block 92, #reads = 1073169 mem = 536881917 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283775, 25, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 369, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2360) [M::mem_pestat] mean and std.dev: (644.59, 634.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (277.14, 144.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 864, 1704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4774) [M::mem_pestat] mean and std.dev: (1097.88, 1011.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6309) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 308, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (351.50, 240.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696374 sequences (100000263 bp) in 3.842 CPU sec, 4.240 real sec... [M::mem_process_seqs] Processed 696384 reads in 116.537 CPU sec, 4.020 real sec sorting block 93, #reads = 1073667 mem = 536879956 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283033, 26, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 412, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3455) [M::mem_pestat] mean and std.dev: (702.38, 679.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (276.21, 144.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 500, 2065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5825) [M::mem_pestat] mean and std.dev: (1037.19, 1104.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 446, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3122) [M::mem_pestat] mean and std.dev: (596.56, 554.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699004 sequences (100000107 bp) in 3.084 CPU sec, 3.563 real sec... [M::mem_process_seqs] Processed 696374 reads in 120.496 CPU sec, 4.192 real sec [M::process] read 703480 sequences (100000086 bp) in 3.305 CPU sec, 3.565 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283828, 30, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 356, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134) [M::mem_pestat] mean and std.dev: (322.88, 163.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 236, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (268.68, 141.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (308, 763, 1467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3785) [M::mem_pestat] mean and std.dev: (768.70, 610.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 277, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (377.56, 313.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 94, #reads = 1072712 mem = 536878119 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286208, 18, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 376, 1369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3737) [M::mem_pestat] mean and std.dev: (529.73, 483.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 225, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (254.26, 131.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (196, 382, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2779) [M::mem_pestat] mean and std.dev: (690.00, 643.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 319, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1392) [M::mem_pestat] mean and std.dev: (374.29, 289.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699004 reads in 224.522 CPU sec, 7.208 real sec [M::process] read 701376 sequences (100000193 bp) in 4.299 CPU sec, 4.786 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 285344, 18, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 377, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (586.92, 548.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 230, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (259.05, 133.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 479, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1758) [M::mem_pestat] mean and std.dev: (563.41, 428.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 243, 1112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2936) [M::mem_pestat] mean and std.dev: (680.94, 834.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701376 reads in 105.783 CPU sec, 3.460 real sec sorting block 95, #reads = 1065300 mem = 536881144 peak = 807546880 [M::process] read 700300 sequences (100000000 bp) in 4.875 CPU sec, 5.380 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 285176, 20, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 506, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1523) [M::mem_pestat] mean and std.dev: (463.00, 218.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (262.73, 134.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 1058, 1653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4217) [M::mem_pestat] mean and std.dev: (1044.47, 956.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 251, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852) [M::mem_pestat] mean and std.dev: (395.48, 329.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703480 reads in 344.962 CPU sec, 12.768 real sec [M::mem_process_seqs] Processed 700300 reads in 107.087 CPU sec, 3.452 real sec [M::process] read 700180 sequences (100000196 bp) in 4.423 CPU sec, 4.847 real sec... sorting block 96, #reads = 1077976 mem = 536878739 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285286, 20, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 304, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (295.49, 123.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (263.33, 135.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (290, 447, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3548) [M::mem_pestat] mean and std.dev: (593.00, 443.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 306, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1440) [M::mem_pestat] mean and std.dev: (365.33, 290.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700180 reads in 113.885 CPU sec, 3.948 real sec [M::process] read 699598 sequences (100000201 bp) in 4.170 CPU sec, 4.605 real sec... sorting block 97, #reads = 1079022 mem = 536881700 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285057, 17, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 627, 1607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4493) [M::mem_pestat] mean and std.dev: (745.95, 683.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (264.90, 136.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (121, 869, 2417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7009) [M::mem_pestat] mean and std.dev: (1100.47, 1094.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 324, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (343.97, 209.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699598 reads in 114.286 CPU sec, 3.906 real sec [M::process] read 699058 sequences (100000036 bp) in 4.150 CPU sec, 4.617 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285022, 38, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 394, 834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (586.18, 553.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (267.43, 137.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 891, 1983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5525) [M::mem_pestat] mean and std.dev: (1207.68, 1248.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 320, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (365.42, 249.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699058 reads in 111.533 CPU sec, 3.829 real sec sorting block 98, #reads = 1077499 mem = 536878872 peak = 807546880 [M::process] read 698912 sequences (100000183 bp) in 4.507 CPU sec, 5.087 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284785, 20, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 452, 1927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5353) [M::mem_pestat] mean and std.dev: (746.78, 690.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7066) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (268.39, 139.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 686, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2270) [M::mem_pestat] mean and std.dev: (635.95, 613.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3089) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (369, 727, 2811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7695) [M::mem_pestat] mean and std.dev: (1072.11, 996.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698912 reads in 113.600 CPU sec, 3.883 real sec [M::process] read 698678 sequences (100000273 bp) in 4.212 CPU sec, 4.715 real sec... sorting block 99, #reads = 1077689 mem = 536878516 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284442, 29, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 602, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3169) [M::mem_pestat] mean and std.dev: (697.06, 579.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (269.09, 139.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 538, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3897) [M::mem_pestat] mean and std.dev: (864.11, 869.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 224, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (315.65, 176.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698892 sequences (100000230 bp) in 3.664 CPU sec, 4.091 real sec... [M::process] read 698444 sequences (100000290 bp) in 1.968 CPU sec, 2.006 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285032, 26, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 842, 2408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6450) [M::mem_pestat] mean and std.dev: (1133.58, 949.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (268.84, 139.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (417, 979, 1962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5052) [M::mem_pestat] mean and std.dev: (1358.32, 1417.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7027) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 244, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (225.33, 67.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697816 sequences (100000087 bp) in 1.950 CPU sec, 1.949 real sec... [M::mem_process_seqs] Processed 698892 reads in 126.721 CPU sec, 4.173 real sec sorting block 100, #reads = 1074934 mem = 536880880 peak = 807546880 [M::mem_process_seqs] Processed 698678 reads in 303.391 CPU sec, 10.043 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284685, 23, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 448, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1564) [M::mem_pestat] mean and std.dev: (376.38, 243.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (270.69, 140.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 335, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (374.95, 215.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1383) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (452, 476, 908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1820) [M::mem_pestat] mean and std.dev: (580.29, 361.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697236 sequences (100000151 bp) in 3.209 CPU sec, 3.800 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284409, 38, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 313, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2655) [M::mem_pestat] mean and std.dev: (558.33, 598.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (273.48, 141.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (503, 1007, 2370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6104) [M::mem_pestat] mean and std.dev: (1441.75, 1305.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7971) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 219, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2262) [M::mem_pestat] mean and std.dev: (466.80, 486.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698444 reads in 222.448 CPU sec, 7.076 real sec sorting block 101, #reads = 1075442 mem = 536880704 peak = 807546880 [M::mem_process_seqs] Processed 697816 reads in 129.924 CPU sec, 4.122 real sec [M::process] read 696544 sequences (100000115 bp) in 3.598 CPU sec, 3.995 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283764, 16, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 1096, 3791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10867) [M::mem_pestat] mean and std.dev: (2035.45, 2500.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (274.29, 141.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 400, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2019) [M::mem_pestat] mean and std.dev: (359.07, 334.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 459, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2283) [M::mem_pestat] mean and std.dev: (558.31, 515.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 102, #reads = 1076491 mem = 536879365 peak = 807546880 [M::process] read 696772 sequences (100000151 bp) in 2.987 CPU sec, 3.247 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283774, 34, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 394, 1353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3559) [M::mem_pestat] mean and std.dev: (833.09, 793.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (275.86, 142.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (301, 1074, 2636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7306) [M::mem_pestat] mean and std.dev: (1747.82, 1995.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 420, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2892) [M::mem_pestat] mean and std.dev: (675.27, 653.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697236 reads in 211.930 CPU sec, 6.773 real sec [M::mem_process_seqs] Processed 696544 reads in 142.311 CPU sec, 4.494 real sec [M::process] read 696326 sequences (100000171 bp) in 3.819 CPU sec, 4.176 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 283541, 27, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 324, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2447) [M::mem_pestat] mean and std.dev: (652.23, 636.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (274.07, 141.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 653, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472) [M::mem_pestat] mean and std.dev: (592.04, 485.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 332, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (282.07, 104.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696772 reads in 124.253 CPU sec, 4.106 real sec sorting block 103, #reads = 1074570 mem = 536878568 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283421, 16, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 255, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2695) [M::mem_pestat] mean and std.dev: (524.74, 499.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (274.80, 141.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (482, 721, 2278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5870) [M::mem_pestat] mean and std.dev: (1302.06, 1206.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 300, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1536) [M::mem_pestat] mean and std.dev: (450.50, 329.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696456 sequences (100000028 bp) in 3.975 CPU sec, 4.296 real sec... [M::mem_process_seqs] Processed 696326 reads in 143.355 CPU sec, 4.745 real sec sorting block 104, #reads = 1073210 mem = 536878602 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 283455, 28, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 419, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2033) [M::mem_pestat] mean and std.dev: (477.57, 375.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (273.63, 140.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (531, 891, 2857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7509) [M::mem_pestat] mean and std.dev: (1802.11, 2075.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10105) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 523, 997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2519) [M::mem_pestat] mean and std.dev: (593.41, 478.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696584 sequences (100000123 bp) in 4.109 CPU sec, 4.527 real sec... [M::mem_process_seqs] Processed 696456 reads in 117.850 CPU sec, 4.121 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283322, 30, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 511, 1561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4103) [M::mem_pestat] mean and std.dev: (927.28, 851.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (273.51, 141.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 442, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2093) [M::mem_pestat] mean and std.dev: (475.15, 279.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 265, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (375.73, 269.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 105, #reads = 1073201 mem = 536877335 peak = 807546880 [M::mem_process_seqs] Processed 696584 reads in 117.205 CPU sec, 4.113 real sec [M::process] read 696626 sequences (100000124 bp) in 4.719 CPU sec, 5.235 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283913, 27, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 346, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2307) [M::mem_pestat] mean and std.dev: (516.06, 480.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (273.48, 140.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 267, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (283.58, 189.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 411, 2654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7556) [M::mem_pestat] mean and std.dev: (1040.03, 1193.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696626 reads in 126.423 CPU sec, 4.331 real sec sorting block 106, #reads = 1071889 mem = 536881995 peak = 807546880 [M::process] read 696482 sequences (100000228 bp) in 4.756 CPU sec, 5.385 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282765, 22, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 387, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (445.70, 339.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (274.25, 141.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 823, 2882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8512) [M::mem_pestat] mean and std.dev: (1352.30, 1996.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 343, 1534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4228) [M::mem_pestat] mean and std.dev: (901.79, 958.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696482 reads in 113.635 CPU sec, 3.848 real sec [M::process] read 697074 sequences (100000259 bp) in 4.494 CPU sec, 5.064 real sec... sorting block 107, #reads = 1069259 mem = 536879182 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283573, 21, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 556, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2796) [M::mem_pestat] mean and std.dev: (630.92, 515.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (273.07, 141.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 375, 1951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5705) [M::mem_pestat] mean and std.dev: (1099.05, 1272.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 363, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2622) [M::mem_pestat] mean and std.dev: (579.42, 544.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697074 reads in 109.241 CPU sec, 3.530 real sec [M::process] read 697170 sequences (100000084 bp) in 3.333 CPU sec, 3.745 real sec... sorting block 108, #reads = 1071806 mem = 536879715 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283700, 15, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 290, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197) [M::mem_pestat] mean and std.dev: (289.19, 157.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (272.70, 141.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (365, 542, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (411.08, 193.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 397, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2099) [M::mem_pestat] mean and std.dev: (542.33, 455.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697900 sequences (100000106 bp) in 3.426 CPU sec, 3.659 real sec... [M::mem_process_seqs] Processed 697170 reads in 113.069 CPU sec, 4.362 real sec [M::process] read 705740 sequences (100000111 bp) in 2.518 CPU sec, 2.727 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 284100, 37, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 420, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2305) [M::mem_pestat] mean and std.dev: (483.82, 473.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (270.81, 140.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (552, 746, 1943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4725) [M::mem_pestat] mean and std.dev: (1105.41, 835.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 458, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2959) [M::mem_pestat] mean and std.dev: (663.62, 560.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705136 sequences (100000278 bp) in 1.643 CPU sec, 1.649 real sec... [M::process] read 704030 sequences (100000218 bp) in 1.660 CPU sec, 1.664 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 286534, 18, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 247, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (266.94, 173.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (244.29, 124.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 706, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3058) [M::mem_pestat] mean and std.dev: (709.17, 514.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3997) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 394, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2946) [M::mem_pestat] mean and std.dev: (517.77, 524.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697900 reads in 209.885 CPU sec, 6.639 real sec sorting block 109, #reads = 1070382 mem = 536882321 peak = 807546880 [M::mem_process_seqs] Processed 705740 reads in 137.967 CPU sec, 4.386 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 286948, 17, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 505, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3423) [M::mem_pestat] mean and std.dev: (600.53, 545.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (247.59, 126.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (257, 674, 1549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4133) [M::mem_pestat] mean and std.dev: (900.94, 885.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 269, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (275.61, 125.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 110, #reads = 1065880 mem = 536878416 peak = 807546880 [M::process] read 702562 sequences (100000047 bp) in 3.026 CPU sec, 3.358 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 286650, 18, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 270, 1982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5576) [M::mem_pestat] mean and std.dev: (934.14, 1254.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (251.38, 128.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 438, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2216) [M::mem_pestat] mean and std.dev: (426.00, 407.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 381, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2520) [M::mem_pestat] mean and std.dev: (434.80, 381.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3295) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704426 sequences (100000054 bp) in 1.860 CPU sec, 1.858 real sec... [M::mem_process_seqs] Processed 705136 reads in 182.087 CPU sec, 5.782 real sec [M::mem_process_seqs] Processed 704030 reads in 118.171 CPU sec, 3.998 real sec sorting block 111, #reads = 1077330 mem = 536879000 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286135, 12, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 634, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2625) [M::mem_pestat] mean and std.dev: (636.90, 448.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 703532 sequences (100000296 bp) in 2.394 CPU sec, 2.568 real sec... [M::mem_pestat] (25, 50, 75) percentile: (153, 226, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (255.20, 130.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 707, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3170) [M::mem_pestat] mean and std.dev: (631.18, 500.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 537, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2706) [M::mem_pestat] mean and std.dev: (714.73, 563.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3518) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702462 sequences (100000002 bp) in 1.840 CPU sec, 1.841 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 287042, 15, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 480, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2140) [M::mem_pestat] mean and std.dev: (440.28, 300.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (249.11, 127.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 572, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2087) [M::mem_pestat] mean and std.dev: (547.07, 369.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2694) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 328, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442) [M::mem_pestat] mean and std.dev: (343.04, 191.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702562 reads in 200.271 CPU sec, 6.604 real sec [M::mem_process_seqs] Processed 704426 reads in 134.884 CPU sec, 4.303 real sec sorting block 112, #reads = 1075877 mem = 536882229 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 286416, 25, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 272, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3160) [M::mem_pestat] mean and std.dev: (459.03, 577.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (252.06, 129.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 289, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4408) [M::mem_pestat] mean and std.dev: (779.17, 903.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 397, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3146) [M::mem_pestat] mean and std.dev: (639.78, 641.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701170 sequences (100000135 bp) in 2.929 CPU sec, 3.123 real sec... [M::process] read 701314 sequences (100000003 bp) in 1.877 CPU sec, 1.878 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285941, 15, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 261, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3814) [M::mem_pestat] mean and std.dev: (601.04, 723.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (254.87, 131.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (395, 726, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4787) [M::mem_pestat] mean and std.dev: (1144.87, 1227.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 401, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2517) [M::mem_pestat] mean and std.dev: (511.90, 419.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703532 reads in 194.421 CPU sec, 6.296 real sec sorting block 113, #reads = 1073011 mem = 536879413 peak = 807546880 [M::mem_process_seqs] Processed 702462 reads in 121.496 CPU sec, 3.967 real sec sorting block 114, #reads = 1074008 mem = 536879783 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285333, 22, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 382, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1755) [M::mem_pestat] mean and std.dev: (360.81, 262.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (259.55, 134.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 463, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071) [M::mem_pestat] mean and std.dev: (505.68, 455.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2687) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 713, 1286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3440) [M::mem_pestat] mean and std.dev: (635.83, 495.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699958 sequences (100000039 bp) in 2.933 CPU sec, 3.133 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 285488, 17, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 395, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3881) [M::mem_pestat] mean and std.dev: (690.74, 698.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (260.33, 135.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 442, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2511) [M::mem_pestat] mean and std.dev: (616.94, 582.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 386, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168) [M::mem_pestat] mean and std.dev: (348.28, 214.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698930 sequences (100000086 bp) in 2.740 CPU sec, 2.737 real sec... [M::mem_process_seqs] Processed 701170 reads in 197.441 CPU sec, 6.373 real sec [M::mem_process_seqs] Processed 701314 reads in 129.301 CPU sec, 4.205 real sec sorting block 115, #reads = 1073259 mem = 536880365 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285256, 22, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 334, 1440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3830) [M::mem_pestat] mean and std.dev: (630.00, 618.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (265.88, 138.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 490, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2468) [M::mem_pestat] mean and std.dev: (434.83, 293.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3200) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (323, 624, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3014) [M::mem_pestat] mean and std.dev: (639.29, 449.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698158 sequences (100000197 bp) in 2.823 CPU sec, 3.014 real sec... [M::process] read 697712 sequences (100000040 bp) in 1.760 CPU sec, 1.756 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284290, 17, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 418, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614) [M::mem_pestat] mean and std.dev: (456.48, 336.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 236, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (268.59, 140.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (970, 1447, 1558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2734) [M::mem_pestat] mean and std.dev: (1109.64, 493.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 454, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2079) [M::mem_pestat] mean and std.dev: (551.93, 460.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699958 reads in 188.547 CPU sec, 6.090 real sec [M::mem_process_seqs] Processed 698930 reads in 121.927 CPU sec, 3.875 real sec sorting block 116, #reads = 1073187 mem = 536878826 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284263, 14, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 368, 2191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6091) [M::mem_pestat] mean and std.dev: (1152.93, 1402.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (269.65, 139.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 623, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2395) [M::mem_pestat] mean and std.dev: (682.86, 460.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 293, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (372.10, 272.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1660) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697286 sequences (100000024 bp) in 3.113 CPU sec, 3.306 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 283063, 16, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 345, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435) [M::mem_pestat] mean and std.dev: (444.05, 335.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (271.36, 139.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (448, 693, 2273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5923) [M::mem_pestat] mean and std.dev: (1033.64, 1136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 340, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3225) [M::mem_pestat] mean and std.dev: (528.75, 642.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4239) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697154 sequences (100000119 bp) in 2.010 CPU sec, 2.017 real sec... [M::mem_process_seqs] Processed 698158 reads in 190.383 CPU sec, 6.133 real sec sorting block 117, #reads = 1072145 mem = 536881641 peak = 807546880 [M::mem_process_seqs] Processed 697712 reads in 124.213 CPU sec, 3.969 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 283487, 18, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 574, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329) [M::mem_pestat] mean and std.dev: (701.39, 575.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (272.49, 140.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (437, 995, 1460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3506) [M::mem_pestat] mean and std.dev: (911.25, 637.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 427, 928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2224) [M::mem_pestat] mean and std.dev: (572.58, 445.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 118, #reads = 1070913 mem = 536883884 peak = 807546880 [M::process] read 696798 sequences (100000174 bp) in 2.895 CPU sec, 3.090 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 283850, 39, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 422, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2304) [M::mem_pestat] mean and std.dev: (588.97, 437.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (272.20, 140.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 236, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378) [M::mem_pestat] mean and std.dev: (257.52, 188.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 432, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215) [M::mem_pestat] mean and std.dev: (449.18, 264.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696400 sequences (100000233 bp) in 1.680 CPU sec, 1.681 real sec... [M::mem_process_seqs] Processed 697286 reads in 190.288 CPU sec, 6.101 real sec [M::mem_process_seqs] Processed 697154 reads in 124.424 CPU sec, 3.956 real sec sorting block 119, #reads = 1072230 mem = 536879332 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 283771, 24, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 315, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (419.24, 305.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (273.28, 140.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 614, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3402) [M::mem_pestat] mean and std.dev: (731.83, 702.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 320, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475) [M::mem_pestat] mean and std.dev: (426.21, 284.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695958 sequences (100000012 bp) in 2.891 CPU sec, 3.091 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 283211, 28, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 439, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264) [M::mem_pestat] mean and std.dev: (685.00, 640.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (273.61, 139.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 318, 1844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5228) [M::mem_pestat] mean and std.dev: (1135.37, 1582.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 331, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3073) [M::mem_pestat] mean and std.dev: (581.89, 707.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695546 sequences (100000175 bp) in 1.660 CPU sec, 1.664 real sec... [M::mem_process_seqs] Processed 696798 reads in 186.459 CPU sec, 5.940 real sec [M::mem_process_seqs] Processed 696400 reads in 119.825 CPU sec, 3.892 real sec sorting block 120, #reads = 1072139 mem = 536882357 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282964, 15, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 321, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452) [M::mem_pestat] mean and std.dev: (362.44, 257.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (274.98, 141.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 491, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3097) [M::mem_pestat] mean and std.dev: (798.07, 780.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 313, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368) [M::mem_pestat] mean and std.dev: (415.79, 317.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695752 sequences (100000160 bp) in 3.096 CPU sec, 3.278 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 282810, 35, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 320, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (314.26, 183.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.72, 141.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 479, 2141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5973) [M::mem_pestat] mean and std.dev: (785.39, 877.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 442, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1342) [M::mem_pestat] mean and std.dev: (412.57, 247.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1716) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695958 reads in 177.400 CPU sec, 5.721 real sec [M::process] read 696310 sequences (100000132 bp) in 2.282 CPU sec, 2.334 real sec... [M::mem_process_seqs] Processed 695546 reads in 123.244 CPU sec, 3.900 real sec sorting block 121, #reads = 1073698 mem = 536879862 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 283300, 26, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 394, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2663) [M::mem_pestat] mean and std.dev: (584.73, 449.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.35, 142.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 547, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3977) [M::mem_pestat] mean and std.dev: (758.46, 629.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 433, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2126) [M::mem_pestat] mean and std.dev: (498.34, 395.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 518604 sequences (74389778 bp) in 2.624 CPU sec, 2.862 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 283691, 26, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 295, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2190) [M::mem_pestat] mean and std.dev: (538.34, 488.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (276.76, 143.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 272, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (284.18, 267.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 423, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2671) [M::mem_pestat] mean and std.dev: (606.86, 517.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695752 reads in 172.302 CPU sec, 5.557 real sec sorting block 122, #reads = 1073202 mem = 536882301 peak = 807546880 [M::mem_process_seqs] Processed 696310 reads in 113.347 CPU sec, 3.665 real sec sorting block 123, #reads = 1073445 mem = 536881435 peak = 807546880 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 210882, 12, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 323, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (335.38, 179.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.95, 140.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 979, 3372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9658) [M::mem_pestat] mean and std.dev: (1422.25, 1654.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12801) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (290, 547, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3938) [M::mem_pestat] mean and std.dev: (904.19, 816.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 518604 reads in 75.195 CPU sec, 2.623 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 133045182 overall: 69540229120 mem 23341.824 user 1118.420 sys 860.571 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_4GR-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1136_4GR-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_4GR-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_4GR-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_4GR-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 860.602 sec; CPU: 24460.247 sec sorting block 124, #reads = 813935 mem = 407517158 peak = 807546880 uniq header count 1 merging 125 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7435771289 output reads: 134158744 bam_mem_sort: 125 calls 85.444 user 2.825 sys 88.421 real bam_write: 125 calls 42.839 user 3.609 sys 48.415 real execute: 1 calls 38.942 user 1.700 sys 908.116 real merge_files: 1 calls 38.905 user 1.673 sys 46.957 real parse_chunk: 1997 calls 93.596 user 23.484 sys 119.947 real read_chunk: 5400 calls 65.144 user 52.604 sys 760.342 real sort_block: 1 calls 52.196 user 51.882 sys 740.106 real write_chunk: 3401 calls 38.522 user 1.306 sys 45.927 real overall: 3118432256 mem 1884.910 user 104.949 sys 908.137 real ls: cannot access '*.cram': No such file or directory