.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L003;0003)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/27/5fb5aca8f0ba8d59b743a30f75edd6/.command.err
Size: 134.9 KB
Task: DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L003;0003)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1838.276 CPU sec, 548.243 real sec
[M::process] read 701958 sequences (100000242 bp) in 0.987 CPU sec, 0.990 real sec...
[M::process] read 701746 sequences (100000093 bp) in 1.279 CPU sec, 1.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291721, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (239.74, 113.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701436 sequences (100000046 bp) in 1.288 CPU sec, 1.293 real sec...
[M::process] read 701466 sequences (100000034 bp) in 1.239 CPU sec, 1.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292331, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 398, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193)
[M::mem_pestat] mean and std.dev: (370.36, 302.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1580)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (240.29, 113.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701958 reads in 150.888 CPU sec, 4.766 real sec
[M::mem_process_seqs] Processed 701746 reads in 84.309 CPU sec, 2.708 real sec
sorting block 0, #reads = 1095399 mem = 536880144 peak = 588005376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291889, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (241.65, 114.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (294, 343, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 717)
[M::mem_pestat] mean and std.dev: (351.70, 142.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RR
[M::process] read 701774 sequences (100000185 bp) in 1.716 CPU sec, 1.799 real sec...
[M::process] read 699958 sequences (100000031 bp) in 1.240 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292100, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.13, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701436 reads in 131.233 CPU sec, 4.180 real sec
[M::mem_process_seqs] Processed 701466 reads in 82.811 CPU sec, 2.653 real sec
sorting block 1, #reads = 1095620 mem = 536880550 peak = 715407360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292361, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 239, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092)
[M::mem_pestat] mean and std.dev: (272.44, 137.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1381)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.77, 114.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698826 sequences (100000288 bp) in 2.397 CPU sec, 2.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291432, 8, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 375, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309)
[M::mem_pestat] mean and std.dev: (361.83, 224.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1686)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.50, 119.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698170 sequences (100000180 bp) in 1.312 CPU sec, 1.325 real sec...
[M::mem_process_seqs] Processed 701774 reads in 137.583 CPU sec, 4.380 real sec
[M::mem_process_seqs] Processed 699958 reads in 88.209 CPU sec, 2.817 real sec
sorting block 2, #reads = 1095093 mem = 536880596 peak = 725237760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290850, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.47, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697748 sequences (100000164 bp) in 2.059 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290866, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (252.91, 121.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697922 sequences (100000046 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_process_seqs] Processed 698826 reads in 143.260 CPU sec, 4.606 real sec
sorting block 3, #reads = 1095163 mem = 536879816 peak = 778559488
[M::mem_process_seqs] Processed 698170 reads in 92.014 CPU sec, 2.944 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290928, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 331, 5228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15324)
[M::mem_pestat] mean and std.dev: (2081.60, 2319.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20372)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (252.94, 121.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 4, #reads = 1094478 mem = 536879772 peak = 840908800
[M::process] read 696330 sequences (100000164 bp) in 2.451 CPU sec, 2.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290860, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.04, 118.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697748 reads in 147.386 CPU sec, 4.769 real sec
[M::mem_process_seqs] Processed 697922 reads in 95.636 CPU sec, 3.067 real sec
[M::process] read 696122 sequences (100000190 bp) in 2.289 CPU sec, 2.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289794, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 233, 2092)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6006)
[M::mem_pestat] mean and std.dev: (916.50, 921.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7963)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.24, 121.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (290, 328, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (188, 443)
[M::mem_pestat] mean and std.dev: (311.75, 26.98)
[M::mem_pestat] low and high boundaries for proper pairs: (137, 494)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 5, #reads = 1093620 mem = 536877419 peak = 840908800
[M::process] read 695616 sequences (100000186 bp) in 2.218 CPU sec, 2.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290451, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (255.64, 121.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696330 reads in 146.343 CPU sec, 4.746 real sec
[M::mem_process_seqs] Processed 696122 reads in 94.764 CPU sec, 3.041 real sec
[M::process] read 696138 sequences (100000249 bp) in 2.144 CPU sec, 2.353 real sec...
sorting block 6, #reads = 1093500 mem = 536879045 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290515, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (251, 383, 1889)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5165)
[M::mem_pestat] mean and std.dev: (948.91, 844.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6803)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (256.41, 121.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695970 sequences (100000109 bp) in 1.902 CPU sec, 2.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290076, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.60, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695616 reads in 148.292 CPU sec, 4.763 real sec
[M::process] read 695620 sequences (100000271 bp) in 1.790 CPU sec, 1.861 real sec...
sorting block 7, #reads = 1092823 mem = 536879476 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289831, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.96, 119.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 285, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (272.69, 103.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RR
[M::process] read 695064 sequences (100000151 bp) in 1.579 CPU sec, 1.583 real sec...
[M::mem_process_seqs] Processed 695970 reads in 82.038 CPU sec, 2.615 real sec
[M::mem_process_seqs] Processed 696138 reads in 214.062 CPU sec, 6.850 real sec
sorting block 8, #reads = 1092479 mem = 536876804 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290089, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (255.04, 120.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 694894 sequences (100000088 bp) in 2.433 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289922, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 234, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (256.44, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695620 reads in 156.714 CPU sec, 5.019 real sec
[M::mem_process_seqs] Processed 695064 reads in 87.511 CPU sec, 2.788 real sec
sorting block 9, #reads = 1092064 mem = 536877332 peak = 840908800
[M::process] read 701418 sequences (100000268 bp) in 2.146 CPU sec, 2.369 real sec...
[M::process] read 701716 sequences (100000004 bp) in 2.110 CPU sec, 2.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291784, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.59, 115.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701418 reads in 77.825 CPU sec, 2.465 real sec
sorting block 10, #reads = 1091971 mem = 536878148 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289764, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 235, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (257.67, 121.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702014 sequences (100000169 bp) in 2.087 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291988, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.28, 115.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694894 reads in 214.422 CPU sec, 6.874 real sec
[M::mem_process_seqs] Processed 701716 reads in 100.907 CPU sec, 3.215 real sec
sorting block 11, #reads = 1094236 mem = 536877884 peak = 840908800
[M::process] read 701112 sequences (100000045 bp) in 2.162 CPU sec, 2.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292094, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 283, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500)
[M::mem_pestat] mean and std.dev: (218.70, 82.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 602)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.70, 116.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700638 sequences (100000122 bp) in 1.934 CPU sec, 2.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291743, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.74, 117.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 401, 966)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2434)
[M::mem_pestat] mean and std.dev: (686.14, 551.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3168)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702014 reads in 140.534 CPU sec, 4.507 real sec
[M::mem_process_seqs] Processed 701112 reads in 90.130 CPU sec, 2.867 real sec
sorting block 12, #reads = 1096379 mem = 536877806 peak = 840908800
[M::process] read 700240 sequences (100000271 bp) in 2.226 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291758, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.66, 118.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1097361 mem = 536876996 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291798, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.19, 120.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699086 sequences (100000077 bp) in 2.183 CPU sec, 2.350 real sec...
[M::mem_process_seqs] Processed 700638 reads in 143.244 CPU sec, 4.647 real sec
[M::mem_process_seqs] Processed 700240 reads in 92.300 CPU sec, 2.953 real sec
[M::process] read 698306 sequences (100000014 bp) in 2.268 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291123, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (251.83, 122.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1096497 mem = 536882104 peak = 840908800
[M::process] read 697796 sequences (100000026 bp) in 1.984 CPU sec, 2.111 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291226, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.77, 123.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699086 reads in 143.888 CPU sec, 4.616 real sec
[M::mem_process_seqs] Processed 698306 reads in 92.820 CPU sec, 2.958 real sec
[M::process] read 697502 sequences (100000148 bp) in 2.020 CPU sec, 2.146 real sec...
sorting block 15, #reads = 1095780 mem = 536877869 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290876, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 433, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852)
[M::mem_pestat] mean and std.dev: (495.90, 313.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2394)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (255.26, 123.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696582 sequences (100000174 bp) in 2.343 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291104, 10, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 199, 663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663)
[M::mem_pestat] mean and std.dev: (258.44, 166.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2163)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (254.81, 122.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (683, 717, 1254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2396)
[M::mem_pestat] mean and std.dev: (823.70, 407.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2967)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1800)
[M::mem_pestat] mean and std.dev: (392.71, 265.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2331)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697796 reads in 146.235 CPU sec, 4.682 real sec
[M::mem_process_seqs] Processed 697502 reads in 94.399 CPU sec, 3.010 real sec
[M::process] read 696226 sequences (100000000 bp) in 1.834 CPU sec, 1.919 real sec...
sorting block 16, #reads = 1094717 mem = 536880005 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290110, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (255.84, 122.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695854 sequences (100000088 bp) in 2.194 CPU sec, 2.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290264, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (256.75, 122.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696582 reads in 148.436 CPU sec, 4.767 real sec
sorting block 17, #reads = 1093908 mem = 536879803 peak = 840908800
[M::mem_process_seqs] Processed 696226 reads in 96.565 CPU sec, 3.084 real sec
[M::process] read 695716 sequences (100000098 bp) in 1.845 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290537, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 427, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106)
[M::mem_pestat] mean and std.dev: (353.15, 200.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1412)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (257.02, 122.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 18, #reads = 1092778 mem = 536879520 peak = 840908800
[M::process] read 696048 sequences (100000287 bp) in 2.206 CPU sec, 2.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 289936, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.47, 120.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695854 reads in 150.322 CPU sec, 4.848 real sec
[M::mem_process_seqs] Processed 695716 reads in 98.538 CPU sec, 3.142 real sec
[M::process] read 696158 sequences (100000045 bp) in 1.857 CPU sec, 1.933 real sec...
sorting block 19, #reads = 1091555 mem = 536878030 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290517, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 476, 969)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2615)
[M::mem_pestat] mean and std.dev: (482.42, 410.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3438)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.69, 120.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695710 sequences (100000280 bp) in 2.361 CPU sec, 2.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290056, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.97, 119.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696048 reads in 147.704 CPU sec, 4.743 real sec
[M::mem_process_seqs] Processed 696158 reads in 95.864 CPU sec, 3.067 real sec
[M::process] read 695988 sequences (100000222 bp) in 1.886 CPU sec, 1.980 real sec...
sorting block 20, #reads = 1092033 mem = 536881087 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 289659, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (254.80, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 574, 817)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071)
[M::mem_pestat] mean and std.dev: (394.89, 272.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2698)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290067, 14, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.31, 121.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 675, 865)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329)
[M::mem_pestat] mean and std.dev: (566.15, 451.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3061)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 700854 sequences (100000090 bp) in 3.176 CPU sec, 3.612 real sec...
[M::mem_process_seqs] Processed 695710 reads in 149.185 CPU sec, 4.819 real sec
[M::mem_process_seqs] Processed 695988 reads in 97.665 CPU sec, 3.147 real sec
sorting block 21, #reads = 1092670 mem = 536878924 peak = 840908800
[M::process] read 702350 sequences (100000107 bp) in 2.391 CPU sec, 2.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291482, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.50, 117.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1092334 mem = 536885411 peak = 840908800
[M::process] read 701518 sequences (100000004 bp) in 2.057 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292307, 1, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.39, 115.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 282, 896)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362)
[M::mem_pestat] mean and std.dev: (516.41, 433.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3095)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700854 reads in 153.161 CPU sec, 4.919 real sec
[M::mem_process_seqs] Processed 702350 reads in 96.787 CPU sec, 3.093 real sec
[M::process] read 700876 sequences (100000006 bp) in 2.217 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292244, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.38, 115.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 23, #reads = 1094603 mem = 536878524 peak = 840908800
[M::process] read 700656 sequences (100000058 bp) in 2.163 CPU sec, 2.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291567, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 508, 1152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3092)
[M::mem_pestat] mean and std.dev: (607.70, 487.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4062)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.94, 116.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701518 reads in 149.687 CPU sec, 4.830 real sec
[M::mem_process_seqs] Processed 700876 reads in 97.941 CPU sec, 3.155 real sec
[M::process] read 699790 sequences (100000174 bp) in 1.909 CPU sec, 2.005 real sec...
sorting block 24, #reads = 1096050 mem = 536881469 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292045, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (246.87, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699100 sequences (100000180 bp) in 2.278 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291127, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.72, 120.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700656 reads in 143.785 CPU sec, 4.610 real sec
[M::mem_process_seqs] Processed 699790 reads in 91.969 CPU sec, 2.926 real sec
[M::process] read 698262 sequences (100000105 bp) in 1.951 CPU sec, 2.055 real sec...
sorting block 25, #reads = 1094732 mem = 536882554 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291562, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.34, 121.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698278 sequences (100000128 bp) in 2.668 CPU sec, 2.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290992, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (253.07, 122.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699100 reads in 142.568 CPU sec, 4.574 real sec
sorting block 26, #reads = 1095201 mem = 536880705 peak = 840908800
[M::mem_process_seqs] Processed 698262 reads in 92.881 CPU sec, 2.973 real sec
[M::process] read 697756 sequences (100000132 bp) in 2.797 CPU sec, 3.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290856, 10, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 298, 1172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3144)
[M::mem_pestat] mean and std.dev: (435.56, 408.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4130)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.93, 122.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 664, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1890)
[M::mem_pestat] mean and std.dev: (410.62, 280.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2503)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698278 reads in 79.636 CPU sec, 2.689 real sec
sorting block 27, #reads = 1095663 mem = 536879516 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290792, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (247, 395, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (305.10, 113.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.73, 122.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697940 sequences (100000277 bp) in 2.427 CPU sec, 2.691 real sec...
[M::mem_process_seqs] Processed 697756 reads in 81.748 CPU sec, 2.743 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291233, 9, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.30, 121.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 282, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (302.08, 136.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RR
sorting block 28, #reads = 1093951 mem = 536877096 peak = 840908800
[M::process] read 697464 sequences (100000241 bp) in 2.511 CPU sec, 2.736 real sec...
[M::mem_process_seqs] Processed 697940 reads in 81.965 CPU sec, 2.730 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290900, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (253.18, 121.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697338 sequences (100000218 bp) in 2.572 CPU sec, 2.804 real sec...
[M::mem_process_seqs] Processed 697464 reads in 83.347 CPU sec, 2.776 real sec
sorting block 29, #reads = 1093187 mem = 536879474 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290604, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.35, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697338 reads in 82.581 CPU sec, 2.741 real sec
[M::process] read 697242 sequences (100000246 bp) in 3.013 CPU sec, 3.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290984, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 245, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425)
[M::mem_pestat] mean and std.dev: (203.20, 56.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.10, 119.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 217, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (243.23, 96.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697242 reads in 81.579 CPU sec, 2.722 real sec
sorting block 30, #reads = 1094106 mem = 536882307 peak = 840908800
[M::process] read 696896 sequences (100000172 bp) in 3.335 CPU sec, 3.750 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290576, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 201, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (180.33, 77.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.35, 119.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 380, 1111)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2965)
[M::mem_pestat] mean and std.dev: (428.44, 380.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3892)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696896 reads in 82.466 CPU sec, 2.741 real sec
[M::process] read 696502 sequences (100000025 bp) in 3.332 CPU sec, 3.747 real sec...
sorting block 31, #reads = 1093753 mem = 536879177 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290149, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.81, 120.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696502 reads in 82.596 CPU sec, 2.720 real sec
[M::process] read 695856 sequences (100000005 bp) in 3.304 CPU sec, 3.729 real sec...
sorting block 32, #reads = 1092145 mem = 536886441 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290122, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 323, 1425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3843)
[M::mem_pestat] mean and std.dev: (638.62, 522.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5052)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.30, 121.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 695856 reads in 81.962 CPU sec, 2.716 real sec
[M::process] read 695744 sequences (100000189 bp) in 2.521 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289700, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (254.80, 120.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695744 reads in 81.372 CPU sec, 2.670 real sec
sorting block 33, #reads = 1091969 mem = 536877925 peak = 840908800
[M::process] read 700526 sequences (100000078 bp) in 3.413 CPU sec, 3.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291536, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.76, 117.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700526 reads in 85.502 CPU sec, 2.794 real sec
[M::process] read 702142 sequences (100000074 bp) in 3.230 CPU sec, 3.642 real sec...
sorting block 34, #reads = 1090627 mem = 536878144 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292175, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 319, 974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2518)
[M::mem_pestat] mean and std.dev: (545.27, 383.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3290)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.44, 116.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702142 reads in 82.138 CPU sec, 2.683 real sec
[M::process] read 701400 sequences (100000207 bp) in 3.199 CPU sec, 3.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291705, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 341, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1291)
[M::mem_pestat] mean and std.dev: (353.80, 219.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.05, 117.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701400 reads in 81.355 CPU sec, 2.663 real sec
[M::process] read 700842 sequences (100000165 bp) in 2.447 CPU sec, 2.712 real sec...
sorting block 35, #reads = 1096794 mem = 536878086 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292262, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.52, 118.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700842 reads in 81.589 CPU sec, 2.668 real sec
[M::process] read 699798 sequences (100000212 bp) in 3.407 CPU sec, 3.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291406, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 507, 1335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3645)
[M::mem_pestat] mean and std.dev: (608.80, 518.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.84, 119.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699798 reads in 80.900 CPU sec, 2.650 real sec
sorting block 36, #reads = 1096802 mem = 536878035 peak = 840908800
[M::process] read 699564 sequences (100000031 bp) in 3.272 CPU sec, 3.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291280, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.29, 120.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699564 reads in 81.406 CPU sec, 2.821 real sec
[M::process] read 698870 sequences (100000075 bp) in 3.150 CPU sec, 3.572 real sec...
sorting block 37, #reads = 1095616 mem = 536878563 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290988, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.58, 121.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698870 reads in 80.451 CPU sec, 2.629 real sec
[M::process] read 698756 sequences (100000187 bp) in 2.626 CPU sec, 2.865 real sec...
sorting block 38, #reads = 1094663 mem = 536876992 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291139, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.77, 122.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698756 reads in 81.357 CPU sec, 2.648 real sec
[M::process] read 698008 sequences (100000199 bp) in 2.517 CPU sec, 2.707 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290977, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.28, 123.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698008 reads in 80.965 CPU sec, 2.643 real sec
sorting block 39, #reads = 1094489 mem = 536886300 peak = 840908800
[M::process] read 698040 sequences (100000074 bp) in 3.670 CPU sec, 4.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291131, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.39, 121.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698040 reads in 82.008 CPU sec, 2.671 real sec
[M::process] read 698416 sequences (100000072 bp) in 3.314 CPU sec, 3.627 real sec...
sorting block 40, #reads = 1093474 mem = 536878309 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290922, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.75, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698416 reads in 81.694 CPU sec, 2.663 real sec
[M::process] read 698322 sequences (100000204 bp) in 2.762 CPU sec, 3.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291063, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.66, 119.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698322 reads in 82.030 CPU sec, 2.682 real sec
[M::process] read 698598 sequences (100000081 bp) in 3.275 CPU sec, 3.723 real sec...
sorting block 41, #reads = 1094094 mem = 536878732 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290997, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.42, 118.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698598 reads in 80.241 CPU sec, 2.616 real sec
[M::process] read 698122 sequences (100000203 bp) in 2.628 CPU sec, 2.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291219, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 246, 1122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2898)
[M::mem_pestat] mean and std.dev: (488.50, 395.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3786)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (248.01, 117.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 309, 558)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (324.69, 118.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1425)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698122 reads in 80.931 CPU sec, 2.649 real sec
sorting block 42, #reads = 1094015 mem = 536878371 peak = 840908800
[M::process] read 697432 sequences (100000240 bp) in 3.419 CPU sec, 3.807 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290360, 11, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (249.52, 118.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 408, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1946)
[M::mem_pestat] mean and std.dev: (479.18, 348.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2534)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 267, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611)
[M::mem_pestat] mean and std.dev: (311.56, 195.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2095)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697054 sequences (100000044 bp) in 2.420 CPU sec, 2.592 real sec...
[M::mem_process_seqs] Processed 697432 reads in 81.261 CPU sec, 2.646 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290783, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 169, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.73, 131.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.31, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 43, #reads = 1093515 mem = 536877456 peak = 840908800
[M::mem_process_seqs] Processed 697054 reads in 79.960 CPU sec, 2.608 real sec
[M::process] read 696898 sequences (100000008 bp) in 3.596 CPU sec, 3.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290261, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.47, 120.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696898 reads in 80.988 CPU sec, 2.643 real sec
sorting block 44, #reads = 1092971 mem = 536880264 peak = 840908800
[M::process] read 696410 sequences (100000300 bp) in 2.912 CPU sec, 3.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290140, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 266, 932)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2358)
[M::mem_pestat] mean and std.dev: (430.08, 337.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3071)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.82, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696410 reads in 80.296 CPU sec, 2.615 real sec
[M::process] read 701382 sequences (100000124 bp) in 2.948 CPU sec, 3.298 real sec...
sorting block 45, #reads = 1092619 mem = 536880330 peak = 840908800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291935, 9, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 249, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (225.55, 84.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (241.90, 115.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701382 reads in 85.517 CPU sec, 2.776 real sec
[M::process] read 706328 sequences (100000174 bp) in 3.189 CPU sec, 3.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293574, 9, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 217, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (201.33, 85.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (228.63, 108.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 732)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 706328 reads in 84.319 CPU sec, 2.755 real sec
sorting block 46, #reads = 1092903 mem = 536876564 peak = 840908800
[M::process] read 705306 sequences (100000013 bp) in 3.193 CPU sec, 3.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293422, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (232.48, 111.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705306 reads in 83.742 CPU sec, 2.725 real sec
[M::process] read 704388 sequences (100000068 bp) in 2.647 CPU sec, 3.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293084, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.29, 113.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 210, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 355)
[M::mem_pestat] mean and std.dev: (184.25, 42.44)
[M::mem_pestat] low and high boundaries for proper pairs: (13, 412)
[M::mem_pestat] skip orientation RR
sorting block 47, #reads = 1096909 mem = 536881700 peak = 840908800
[M::mem_process_seqs] Processed 704388 reads in 83.782 CPU sec, 2.734 real sec
[M::process] read 704598 sequences (100000086 bp) in 3.749 CPU sec, 4.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 293517, 14, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 202, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (196.50, 48.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (232.64, 111.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (511, 2967, 5954)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16840)
[M::mem_pestat] mean and std.dev: (2850.21, 2553.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 703302 sequences (100000113 bp) in 2.429 CPU sec, 2.537 real sec...
[M::process] read 702658 sequences (100000128 bp) in 1.690 CPU sec, 1.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292586, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (237.68, 115.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703302 reads in 83.379 CPU sec, 2.635 real sec
sorting block 48, #reads = 1096706 mem = 536879878 peak = 846786560
[M::process] read 701598 sequences (100000046 bp) in 2.149 CPU sec, 2.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292594, 12, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.50, 117.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 524, 873)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2133)
[M::mem_pestat] mean and std.dev: (532.50, 368.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2763)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 704598 reads in 233.559 CPU sec, 7.532 real sec
[M::mem_process_seqs] Processed 702658 reads in 97.763 CPU sec, 3.201 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292145, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.88, 119.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701598 reads in 85.037 CPU sec, 2.856 real sec
[M::process] read 702610 sequences (100000081 bp) in 2.900 CPU sec, 3.328 real sec...
sorting block 49, #reads = 1096359 mem = 536882040 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292870, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.33, 115.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702610 reads in 84.977 CPU sec, 2.844 real sec
sorting block 50, #reads = 1095370 mem = 536880969 peak = 869097472
[M::process] read 700950 sequences (100000028 bp) in 3.162 CPU sec, 3.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291838, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (243.46, 117.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700950 reads in 85.589 CPU sec, 2.838 real sec
[M::process] read 700940 sequences (100000035 bp) in 3.228 CPU sec, 3.761 real sec...
sorting block 51, #reads = 1095345 mem = 536879715 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292283, 6, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (262, 304, 753)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1735)
[M::mem_pestat] mean and std.dev: (401.33, 224.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2226)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.48, 119.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700940 reads in 85.889 CPU sec, 2.834 real sec
[M::process] read 699344 sequences (100000264 bp) in 3.575 CPU sec, 4.000 real sec...
sorting block 52, #reads = 1093220 mem = 536883893 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291670, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (249.28, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (188.70, 47.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699344 reads in 86.222 CPU sec, 2.813 real sec
[M::process] read 698348 sequences (100000104 bp) in 3.528 CPU sec, 3.889 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291126, 9, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.47, 122.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 306, 832)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1990)
[M::mem_pestat] mean and std.dev: (441.17, 275.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2569)
[M::mem_pestat] skip orientation RR
[M::process] read 696722 sequences (100000207 bp) in 2.506 CPU sec, 2.698 real sec...
[M::mem_process_seqs] Processed 698348 reads in 86.618 CPU sec, 2.808 real sec
sorting block 53, #reads = 1092248 mem = 536878893 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290532, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.57, 122.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (314, 357, 946)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210)
[M::mem_pestat] mean and std.dev: (554.60, 419.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2842)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696722 reads in 87.361 CPU sec, 2.854 real sec
[M::process] read 696730 sequences (100000101 bp) in 3.192 CPU sec, 3.594 real sec...
sorting block 54, #reads = 1091917 mem = 536879679 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290323, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 222, 959)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2553)
[M::mem_pestat] mean and std.dev: (499.27, 637.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3350)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.57, 121.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696730 reads in 85.604 CPU sec, 2.800 real sec
[M::process] read 696604 sequences (100000157 bp) in 3.341 CPU sec, 3.728 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290481, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.52, 122.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696604 reads in 84.459 CPU sec, 2.747 real sec
[M::process] read 696608 sequences (100000029 bp) in 2.564 CPU sec, 2.795 real sec...
sorting block 55, #reads = 1090234 mem = 536882608 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290214, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (255.37, 123.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 350, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (270.18, 85.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696608 reads in 86.374 CPU sec, 2.820 real sec
[M::process] read 708072 sequences (100000215 bp) in 3.640 CPU sec, 4.056 real sec...
sorting block 56, #reads = 1089662 mem = 536880403 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 294035, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 202, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (224.64, 105.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708304 sequences (100000181 bp) in 2.337 CPU sec, 2.677 real sec...
[M::mem_process_seqs] Processed 708072 reads in 90.805 CPU sec, 2.946 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 294238, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 202, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (225.19, 106.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705954 sequences (100000041 bp) in 2.854 CPU sec, 3.182 real sec...
[M::mem_process_seqs] Processed 708304 reads in 93.553 CPU sec, 3.030 real sec
sorting block 57, #reads = 1091159 mem = 536881854 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 293796, 9, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (230.82, 109.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 236, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (249.36, 75.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 705954 reads in 89.439 CPU sec, 2.916 real sec
[M::process] read 704776 sequences (100000301 bp) in 3.533 CPU sec, 3.915 real sec...
sorting block 58, #reads = 1091193 mem = 536878684 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293118, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.83, 112.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703226 sequences (100000147 bp) in 2.693 CPU sec, 2.842 real sec...
[M::mem_process_seqs] Processed 704776 reads in 90.406 CPU sec, 2.926 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293241, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.20, 114.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703226 reads in 87.218 CPU sec, 2.846 real sec
[M::process] read 702744 sequences (100000296 bp) in 3.531 CPU sec, 3.900 real sec...
sorting block 59, #reads = 1091447 mem = 536881390 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292506, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.79, 115.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702744 reads in 86.080 CPU sec, 2.804 real sec
[M::process] read 701888 sequences (100000103 bp) in 2.755 CPU sec, 3.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292268, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.43, 117.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1094283 mem = 536879892 peak = 869097472
[M::mem_process_seqs] Processed 701888 reads in 91.636 CPU sec, 2.975 real sec
[M::process] read 701426 sequences (100000068 bp) in 2.997 CPU sec, 3.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292237, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.28, 117.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701426 reads in 87.619 CPU sec, 2.858 real sec
sorting block 61, #reads = 1089186 mem = 536878753 peak = 869097472
[M::process] read 701036 sequences (100000106 bp) in 3.616 CPU sec, 4.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292285, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (334, 419, 2981)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8275)
[M::mem_pestat] mean and std.dev: (1634.55, 2166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10922)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.48, 118.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700946 sequences (100000183 bp) in 2.340 CPU sec, 2.534 real sec...
[M::mem_process_seqs] Processed 701036 reads in 85.728 CPU sec, 2.777 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292166, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.51, 119.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700946 reads in 86.994 CPU sec, 2.836 real sec
sorting block 62, #reads = 1092761 mem = 536879836 peak = 869097472
[M::process] read 700040 sequences (100000253 bp) in 3.227 CPU sec, 3.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291646, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (247.16, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700040 reads in 84.987 CPU sec, 2.761 real sec
[M::process] read 698702 sequences (100000121 bp) in 3.094 CPU sec, 3.454 real sec...
sorting block 63, #reads = 1091052 mem = 536879999 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291032, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.75, 123.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698702 reads in 84.401 CPU sec, 2.758 real sec
[M::process] read 697440 sequences (100000202 bp) in 2.522 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290902, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.91, 123.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 313, 1596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4408)
[M::mem_pestat] mean and std.dev: (1043.64, 1132.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5814)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697440 reads in 83.999 CPU sec, 2.741 real sec
[M::process] read 698190 sequences (100000255 bp) in 3.081 CPU sec, 3.476 real sec...
sorting block 64, #reads = 1091737 mem = 536877790 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291061, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.73, 122.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698190 reads in 85.437 CPU sec, 2.796 real sec
[M::process] read 698310 sequences (100000182 bp) in 2.515 CPU sec, 2.814 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291130, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.52, 120.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 228, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329)
[M::mem_pestat] mean and std.dev: (281.55, 152.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1703)
[M::mem_pestat] skip orientation RR
sorting block 65, #reads = 1090902 mem = 536879020 peak = 869097472
[M::mem_process_seqs] Processed 698310 reads in 85.719 CPU sec, 2.798 real sec
[M::process] read 699078 sequences (100000067 bp) in 3.236 CPU sec, 3.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290673, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 353, 997)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2633)
[M::mem_pestat] mean and std.dev: (362.82, 313.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3451)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.78, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699078 reads in 95.173 CPU sec, 3.089 real sec
[M::process] read 706956 sequences (100000069 bp) in 2.713 CPU sec, 3.120 real sec...
sorting block 66, #reads = 1090050 mem = 536878718 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293590, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.32, 108.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 1308, 1308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3404)
[M::mem_pestat] mean and std.dev: (1037.90, 802.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4452)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 706956 reads in 86.046 CPU sec, 2.817 real sec
[M::process] read 705228 sequences (100000185 bp) in 3.076 CPU sec, 3.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293127, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 291, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 503)
[M::mem_pestat] mean and std.dev: (226.62, 75.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 597)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (232.31, 110.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 67, #reads = 1087874 mem = 536881141 peak = 869097472
[M::mem_process_seqs] Processed 705228 reads in 84.867 CPU sec, 2.765 real sec
[M::process] read 703734 sequences (100000187 bp) in 3.055 CPU sec, 3.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292427, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (236.34, 113.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703734 reads in 83.850 CPU sec, 2.730 real sec
[M::process] read 703452 sequences (100000033 bp) in 2.558 CPU sec, 2.783 real sec...
sorting block 68, #reads = 1095240 mem = 536875336 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292756, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.76, 114.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702400 sequences (100000074 bp) in 2.384 CPU sec, 2.651 real sec...
[M::mem_process_seqs] Processed 703452 reads in 81.908 CPU sec, 2.666 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292522, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.69, 116.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 283, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378)
[M::mem_pestat] mean and std.dev: (282.09, 126.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1765)
[M::mem_pestat] skip orientation RR
sorting block 69, #reads = 1094433 mem = 536881804 peak = 869097472
[M::mem_process_seqs] Processed 702400 reads in 82.083 CPU sec, 2.675 real sec
[M::process] read 701870 sequences (100000214 bp) in 2.515 CPU sec, 2.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292196, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 232, 755)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1889)
[M::mem_pestat] mean and std.dev: (401.80, 273.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2456)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.75, 117.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701870 reads in 82.812 CPU sec, 2.712 real sec
[M::process] read 701390 sequences (100000016 bp) in 2.831 CPU sec, 3.143 real sec...
sorting block 70, #reads = 1096194 mem = 536879618 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291857, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.63, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701390 reads in 85.113 CPU sec, 2.772 real sec
[M::process] read 701238 sequences (100000022 bp) in 2.678 CPU sec, 2.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292488, 9, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (302, 334, 877)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027)
[M::mem_pestat] mean and std.dev: (551.10, 399.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2602)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.85, 119.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701238 reads in 84.448 CPU sec, 2.765 real sec
sorting block 71, #reads = 1093713 mem = 536878199 peak = 869097472
[M::process] read 700250 sequences (100000002 bp) in 3.330 CPU sec, 3.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291771, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 316, 2228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6052)
[M::mem_pestat] mean and std.dev: (777.50, 786.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7964)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.88, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (293, 293, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (197, 437)
[M::mem_pestat] mean and std.dev: (305.50, 20.57)
[M::mem_pestat] low and high boundaries for proper pairs: (149, 485)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700250 reads in 84.827 CPU sec, 2.765 real sec
[M::process] read 699810 sequences (100000059 bp) in 2.610 CPU sec, 2.872 real sec...
sorting block 72, #reads = 1093529 mem = 536878265 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291599, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (249.16, 121.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699810 reads in 84.610 CPU sec, 2.756 real sec
[M::process] read 699406 sequences (100000192 bp) in 2.661 CPU sec, 2.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291220, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 436, 751)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741)
[M::mem_pestat] mean and std.dev: (484.80, 241.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2236)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.98, 120.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698490 sequences (100000061 bp) in 2.510 CPU sec, 2.780 real sec...
[M::mem_process_seqs] Processed 699406 reads in 86.198 CPU sec, 2.817 real sec
sorting block 73, #reads = 1092992 mem = 536880600 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290893, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 209, 2263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6437)
[M::mem_pestat] mean and std.dev: (1150.30, 1435.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8524)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.36, 120.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698490 reads in 86.139 CPU sec, 2.805 real sec
[M::process] read 698560 sequences (100000100 bp) in 3.226 CPU sec, 3.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291155, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 327, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488)
[M::mem_pestat] mean and std.dev: (287.62, 155.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1939)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.62, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 74, #reads = 1090378 mem = 536879130 peak = 869097472
[M::mem_process_seqs] Processed 698560 reads in 87.046 CPU sec, 2.843 real sec
[M::process] read 698960 sequences (100000017 bp) in 3.127 CPU sec, 3.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291129, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.24, 119.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698820 sequences (100000036 bp) in 2.330 CPU sec, 2.506 real sec...
[M::mem_process_seqs] Processed 698960 reads in 88.587 CPU sec, 2.875 real sec
sorting block 75, #reads = 1091083 mem = 536876979 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291295, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 192, 755)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1941)
[M::mem_pestat] mean and std.dev: (445.27, 525.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2547)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.11, 119.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698820 reads in 87.478 CPU sec, 2.853 real sec
[M::process] read 698702 sequences (100000156 bp) in 2.992 CPU sec, 3.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290969, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.81, 119.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 247, 957)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593)
[M::mem_pestat] mean and std.dev: (389.25, 471.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3411)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698702 reads in 87.671 CPU sec, 2.856 real sec
sorting block 76, #reads = 1091074 mem = 536879605 peak = 869097472
[M::process] read 701446 sequences (100000076 bp) in 3.194 CPU sec, 3.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291128, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.74, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701446 reads in 98.761 CPU sec, 3.224 real sec
[M::process] read 709552 sequences (100000001 bp) in 3.209 CPU sec, 3.710 real sec...
sorting block 77, #reads = 1088761 mem = 536877478 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 294254, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 200, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (221.50, 103.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 179, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (210.27, 113.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 709552 reads in 92.572 CPU sec, 3.007 real sec
[M::process] read 708730 sequences (100000097 bp) in 3.261 CPU sec, 3.619 real sec...
sorting block 78, #reads = 1086508 mem = 536882925 peak = 869097472
[M::process] read 706094 sequences (100000137 bp) in 2.320 CPU sec, 2.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 294146, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (224.43, 105.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 253, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (298.40, 170.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RR
[M::process] read 704866 sequences (100000097 bp) in 1.530 CPU sec, 1.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293578, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.65, 110.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 708730 reads in 166.536 CPU sec, 5.295 real sec
[M::process] read 703498 sequences (100000084 bp) in 1.740 CPU sec, 1.785 real sec...
[M::mem_process_seqs] Processed 706094 reads in 105.840 CPU sec, 3.354 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293448, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (234.76, 112.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 79, #reads = 1090744 mem = 536882079 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292546, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.57, 114.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702452 sequences (100000182 bp) in 3.029 CPU sec, 3.327 real sec...
[M::mem_process_seqs] Processed 704866 reads in 159.988 CPU sec, 5.170 real sec
[M::mem_process_seqs] Processed 703498 reads in 104.420 CPU sec, 3.346 real sec
sorting block 80, #reads = 1092131 mem = 536882860 peak = 869097472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292563, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.92, 117.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701352 sequences (100000249 bp) in 3.216 CPU sec, 3.620 real sec...
[M::mem_process_seqs] Processed 702452 reads in 89.982 CPU sec, 2.973 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292204, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.94, 119.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 275, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (271.56, 82.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RR
sorting block 81, #reads = 1091341 mem = 536880293 peak = 876138496
[M::mem_process_seqs] Processed 701352 reads in 88.776 CPU sec, 2.922 real sec
[M::process] read 701004 sequences (100000027 bp) in 3.465 CPU sec, 3.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292165, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.71, 118.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 314, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580)
[M::mem_pestat] mean and std.dev: (257.20, 118.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701004 reads in 88.460 CPU sec, 2.906 real sec
sorting block 82, #reads = 1091560 mem = 536876660 peak = 876138496
[M::process] read 700088 sequences (100000134 bp) in 3.197 CPU sec, 3.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291422, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.89, 117.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700050 sequences (100000150 bp) in 2.575 CPU sec, 2.833 real sec...
[M::mem_process_seqs] Processed 700088 reads in 88.098 CPU sec, 2.858 real sec
sorting block 83, #reads = 1091672 mem = 536878716 peak = 876138496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291286, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.53, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 169, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (171.62, 43.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700050 reads in 86.755 CPU sec, 2.827 real sec
[M::process] read 700232 sequences (100000001 bp) in 3.614 CPU sec, 4.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291386, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (243.80, 117.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700232 reads in 87.180 CPU sec, 2.833 real sec
sorting block 84, #reads = 1090638 mem = 536879655 peak = 876138496
[M::process] read 699314 sequences (100000199 bp) in 2.982 CPU sec, 3.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291538, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.72, 119.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699314 reads in 85.738 CPU sec, 2.794 real sec
[M::process] read 698930 sequences (100000099 bp) in 3.543 CPU sec, 3.924 real sec...
sorting block 85, #reads = 1090720 mem = 536876316 peak = 876138496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291090, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (246.93, 118.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698930 reads in 84.557 CPU sec, 2.747 real sec
[M::process] read 698926 sequences (100000021 bp) in 2.764 CPU sec, 3.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291307, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 427, 716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1806)
[M::mem_pestat] mean and std.dev: (358.90, 224.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2351)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.99, 119.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (313, 532, 2115)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5719)
[M::mem_pestat] mean and std.dev: (1012.58, 956.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7521)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698926 reads in 83.933 CPU sec, 2.731 real sec
sorting block 86, #reads = 1092819 mem = 536877699 peak = 876138496
[M::process] read 698280 sequences (100000148 bp) in 3.478 CPU sec, 3.832 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290864, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.65, 120.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698280 reads in 84.586 CPU sec, 2.761 real sec
[M::process] read 647616 sequences (92693920 bp) in 2.630 CPU sec, 2.982 real sec...
sorting block 87, #reads = 1092068 mem = 536879680 peak = 876138496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 270027, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.58, 120.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 647616 reads in 77.652 CPU sec, 2.581 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96538732
overall: 69591060480 mem 12627.216 user 788.358 sys 983.580 real
sorting block 88, #reads = 832901 mem = 410183338 peak = 876138496
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 983.629 sec; CPU: 13415.594 sec
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 5180783496
output reads: 97030513
bam_mem_sort: 89 calls 35.035 user 0.593 sys 35.775 real
bam_write: 89 calls 77.966 user 2.261 sys 80.752 real
execute: 1 calls 22.342 user 1.867 sys 1013.181 real
merge_files: 1 calls 22.322 user 1.837 sys 28.389 real
parse_chunk: 1421 calls 44.239 user 14.368 sys 60.258 real
read_chunk: 3837 calls 51.292 user 46.054 sys 444.466 real
sort_block: 1 calls 42.120 user 45.223 sys 430.762 real
write_chunk: 2414 calls 21.856 user 1.267 sys 27.084 real
overall: 2814865408 mem 1091.097 user 77.416 sys 1013.203 real
ls: cannot access '*.cram': No such file or directory